pycom10g24850

Sphingolipid delta(4)-desaturase DES1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0015507
Physical Location & Seq
Reverse (-)
26582898 .. 26584670
1773 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 987 bp
ATGGGAAGAGGGGGTGAGAGAGAGGCAGCAGAAGAGGAGGGTGTGATGGCTACAGACTTCTTCTGGTCCTACACGGATGAGCCTCACGCTTCTAGGCGCCGTCAGATCCTCTCTCAGTACCCTCAGATTAAGGAGCTCTTCGGTCCCGACCCATGGGCTTTCCTCAAGATTACGGTGGTTGTGTTACTTCAGCTCGGGACTGCTACAGCCCTCCACAATGCAGGCTGGCTGAAGATAGTAGCGATAGCCTACTTTTTTGGCTCATTTCTCAACCACAACCTCTTCTTGGCCATCCATGAGCTCAGTCACAATCTCGCCTTCTCGACCCCAGTCTACAACCGTTGGCTTGGTATTTTTGCTAACCTCCCGATTGGTGTTCCCATGTCTGTCACCTTTCAAAAATATCACCTTGAGCACCACCGCTTCCAGGGAGTAGACGGCATTGATATGGACATCCCGAGCAAGGTTGAGGCTCATGTGGTGACAAATGTGGTCACGAAATCCATTTGGGTTCTCCTTCAACTCTTCTTCTATGCCCTTCGTCCTGTGTTTCTCAAACCGAAACCCCCTGGTTGTTGGGAGTTCCTCAACCTCTTCATTCAGATAGCCCTCGATGTTGCAATGGTCTGCTTTTGGGGCTGGAAATCTTTTGCTTACTTGATCCTCTCTACATTTGTCGGCGGTGGGATGCACCCAATGGCGGGTCACTTCATTTCGGAGCATTATGTCTTCAACCCTGATCAAGAGACGTATTCTTACTATGGCCCTCTCAATCTTCTAGCTTGGCACGTAGGCTACCACAACGAGCACCATGATTTCCCCAGAATTCCCGGGAGCAAGCTTCATAAGGTGAAGGACATTGCACCGGAATACTACGAGGGTTTGGAGTCGTATAAATCTTGGAGCCAAGTGATCTACATGTACATAGCGGATCGAACAGTTGGACCGTTCAGTCGAATGAAGAGAAAGGCAACAAAATCTGAATAG

Protein Analysis

329

Amino Acids

37.75

Weight (kDa)

7.0

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipid_DES PF08557 17 - 52 3.3e-20 Sphingolipid Delta4-desaturase (DES)
FA_desaturase PF00487 75 - 293 1.9e-23 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015507)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G04930
fragaria_vesca FvH4_3g04930
malus_domestica MD10G1295900.v1.1
prunus_persica Prupe.4G044600_v2.0.a1
pyrus_communis pycom10g24850
rosa_chinensis RchiOBHm_Chr5g0008091
rosa_laevigata RLG00000031535
rosa_multiflora Rmu_sc0017465.1_g000005 Rmu_sc0039673.1_g000001
rosa_roxburghii Rroxscaffold_1G00068390
rosa_rugosa Rorug04G0433300
rosa_samantha Rh5AG061400 Rh5BG058200 Rh5CG068300 Rh5DG057500
rosa_wichuraiana Rw5G005920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 951
AccB1I GGYRCC 1 cut(s) 96
AccI GTMKAC 2 cut(s) 333, 435
AciI CCGC 4 cut(s) 421, 681, 701, 929
AclWI GGATC 3 cut(s) 100, 655, 939
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 1 cut(s) 825
AcuI CTGAAG 2 cut(s) 173, 251
AcyI GRCGYC 1 cut(s) 97
AfaI GTAC 2 cut(s) 119, 923
AfiI CCNNNNNNNGG 6 cut(s) 153, 286, 427, 463, 700, 701
AflIII ACRYGT 1 cut(s) 918
AgsI TTSAA 3 cut(s) 398, 521, 733
AjnI CCWGG 2 cut(s) 426, 568
AluBI AGCT 5 cut(s) 136, 193, 301, 782, 841
AluI AGCT 5 cut(s) 136, 193, 301, 782, 841
Alw21I GWGCWC 4 cut(s) 138, 303, 417, 810
Alw26I GTCTC 1 cut(s) 740
AlwI GGATC 3 cut(s) 100, 655, 939
Ama87I CYCGRG 3 cut(s) 194, 457, 830
AoxI GGCC 2 cut(s) 288, 763
ApeKI GCWGC 1 cut(s) 26
ApoI RAATTY 1 cut(s) 825
AspLEI GCGC 1 cut(s) 99
AspS9I GGNCC 4 cut(s) 66, 143, 764, 944
AsuC2I CCSGG 2 cut(s) 831, 832
AsuHPI GGTGA 5 cut(s) 26, 382, 398, 493, 862
AvaI CYCGRG 3 cut(s) 194, 457, 830
AvaII GGWCC 3 cut(s) 66, 143, 944
BalI TGGCCA 1 cut(s) 290
BanI GGYRCC 1 cut(s) 96
BanII GRGCYC 2 cut(s) 138, 303
BbsI GAAGAC 1 cut(s) 721
Bbv12I GWGCWC 4 cut(s) 138, 303, 417, 810
BbvI GCAGC 1 cut(s) 38
BccI CCATC 2 cut(s) 40, 299
BceAI ACGGC 2 cut(s) 84, 454
BciT130I CCWGG 2 cut(s) 428, 570
BclI TGATCA 1 cut(s) 739
BcnI CCSGG 2 cut(s) 831, 832
BcoDI GTCTC 1 cut(s) 740
BfaI CTAG 2 cut(s) 93, 779
BfmI CTRYAG 2 cut(s) 51, 204
BfoI RGCGCY 1 cut(s) 100
BisI GCNGC 1 cut(s) 27
BlsI GCNGC 1 cut(s) 28
Bme1390I CCNGG 4 cut(s) 428, 570, 831, 832
Bme18I GGWCC 3 cut(s) 66, 143, 944
BmeT110I CYCGRG 3 cut(s) 194, 457, 830
BmgT120I GGNCC 4 cut(s) 66, 143, 764, 944
BmiI GGNNCC 3 cut(s) 98, 145, 905
BmrFI CCNGG 4 cut(s) 428, 570, 831, 832
BmrI ACTGGG 1 cut(s) 323
BmsI GCATC 1 cut(s) 678
BmuI ACTGGG 1 cut(s) 323
BoxI GACNNNNGTC 1 cut(s) 329
BpiI GAAGAC 1 cut(s) 721
BpuEI CTTGAG 2 cut(s) 149, 431
BpuMI CCSGG 2 cut(s) 831, 832
BsaAI YACGTR 1 cut(s) 790
BsaHI GRCGYC 1 cut(s) 97
BsaJI CCNNGG 4 cut(s) 152, 427, 568, 830
BsaWI WCCGGW 1 cut(s) 865
Bsc4I CCNNNNNNNGG 6 cut(s) 153, 286, 427, 463, 700, 701
Bse1I ACTGG 1 cut(s) 329
Bse3DI GCAATG 2 cut(s) 627, 858
BseBI CCWGG 2 cut(s) 428, 570
BseDI CCNNGG 4 cut(s) 152, 427, 568, 830
BseGI GGATG 4 cut(s) 82, 291, 453, 693
BseLI CCNNNNNNNGG 6 cut(s) 153, 286, 427, 463, 700, 701
BseMI GCAATG 2 cut(s) 627, 858
BseMII CTCAG 3 cut(s) 128, 137, 316
BseNI ACTGG 1 cut(s) 329
BseRI GAGGAG 1 cut(s) 50
BseXI GCAGC 1 cut(s) 38
BshFI GGCC 2 cut(s) 290, 765
BshNI GGYRCC 1 cut(s) 96
BsiHKAI GWGCWC 4 cut(s) 138, 303, 417, 810
BsiHKCI CYCGRG 3 cut(s) 194, 457, 830
BsiSI CCGG 2 cut(s) 831, 866
BslFI GGGAC 2 cut(s) 129, 211
BslI CCNNNNNNNGG 6 cut(s) 153, 286, 427, 463, 700, 701
BsmAI GTCTC 1 cut(s) 740
BsmBI CGTCTC 1 cut(s) 740
BsmFI GGGAC 2 cut(s) 129, 211
BsnI GGCC 2 cut(s) 290, 765
BsoBI CYCGRG 3 cut(s) 194, 457, 830
Bsp1286I GDGCHC 4 cut(s) 138, 303, 417, 810
Bsp1407I TGTACA 1 cut(s) 921
Bsp143I GATC 5 cut(s) 105, 660, 739, 912, 931
Bsp19I CCATGG 1 cut(s) 152
BspACI CCGC 4 cut(s) 421, 681, 701, 929
BspANI GGCC 2 cut(s) 290, 765
BspCNI CTCAG 3 cut(s) 127, 136, 315
BspLI GGNNCC 3 cut(s) 98, 145, 905
BspPI GGATC 3 cut(s) 100, 655, 939
BspQI GCTCTTC 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 96
BsrDI GCAATG 2 cut(s) 627, 858
BsrGI TGTACA 1 cut(s) 921
BsrI ACTGG 1 cut(s) 329
BssECI CCNNGG 4 cut(s) 152, 427, 568, 830
BssMI GATC 5 cut(s) 105, 660, 739, 912, 931
BssNI GRCGYC 1 cut(s) 97
BssT1I CCWWGG 1 cut(s) 152
Bst2UI CCWGG 2 cut(s) 428, 570
Bst4CI ACNGT 4 cut(s) 175, 341, 940, 948
Bst6I CTCTTC 6 cut(s) 27, 143, 287, 530, 599, 956
BstACI GRCGYC 1 cut(s) 97
BstAUI TGTACA 1 cut(s) 921
BstBAI YACGTR 1 cut(s) 790
BstC8I GCNNGC 3 cut(s) 223, 227, 839
BstDEI CTNAG 3 cut(s) 114, 123, 302
BstDSI CCRYGG 1 cut(s) 152
BstF5I GGATG 4 cut(s) 82, 291, 453, 693
BstH2I RGCGCY 1 cut(s) 100
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 5 cut(s) 108, 663, 742, 915, 934
BstMAI GTCTC 1 cut(s) 740
BstMBI GATC 5 cut(s) 105, 660, 739, 912, 931
BstMWI GCNNNNNNNGC 1 cut(s) 636
BstNI CCWGG 2 cut(s) 428, 570
BstNSI RCATGY 1 cut(s) 922
BstPAI GACNNNNGTC 1 cut(s) 329
BstSCI CCNGG 4 cut(s) 426, 568, 829, 830
BstSFI CTRYAG 2 cut(s) 51, 204
BstV1I GCAGC 1 cut(s) 38
BstV2I GAAGAC 1 cut(s) 721
BstX2I RGATCY 1 cut(s) 105
BstYI RGATCY 1 cut(s) 105
BsuRI GGCC 2 cut(s) 290, 765
BtgI CCRYGG 1 cut(s) 152
BtsCI GGATG 4 cut(s) 82, 291, 453, 693
Cac8I GCNNGC 3 cut(s) 223, 227, 839
CfoI GCGC 1 cut(s) 99
Cfr13I GGNCC 4 cut(s) 66, 143, 764, 944
Cfr9I CCCGGG 1 cut(s) 830
Csp6I GTAC 2 cut(s) 118, 922
CviAII CATG 6 cut(s) 153, 296, 382, 476, 812, 919
CviQI GTAC 2 cut(s) 118, 922
DdeI CTNAG 3 cut(s) 114, 123, 302
DinI GGCGCC 1 cut(s) 98
DpnI GATC 5 cut(s) 107, 662, 741, 914, 933
DpnII GATC 5 cut(s) 105, 660, 739, 912, 931
DrdI GACNNNNNNGTC 1 cut(s) 951
DseDI GACNNNNNNGTC 1 cut(s) 951
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 6 cut(s) 27, 143, 287, 530, 599, 956
EarI CTCTTC 6 cut(s) 27, 143, 287, 530, 599, 956
Ecl136II GAGCTC 2 cut(s) 136, 301
Eco130I CCWWGG 1 cut(s) 152
Eco24I GRGCYC 2 cut(s) 138, 303
Eco47I GGWCC 3 cut(s) 66, 143, 944
Eco53kI GAGCTC 2 cut(s) 136, 301
Eco57I CTGAAG 2 cut(s) 173, 251
Eco88I CYCGRG 3 cut(s) 194, 457, 830
EcoICRI GAGCTC 2 cut(s) 136, 301
EcoRI GAATTC 1 cut(s) 825
EcoRII CCWGG 2 cut(s) 426, 568
EcoT14I CCWWGG 1 cut(s) 152
EcoT38I GRGCYC 2 cut(s) 138, 303
EgeI GGCGCC 1 cut(s) 98
EheI GGCGCC 1 cut(s) 98
ErhI CCWWGG 1 cut(s) 152
Esp3I CGTCTC 1 cut(s) 740
FaeI CATG 6 cut(s) 156, 299, 385, 479, 815, 922
FalI AAGNNNNNCTT 2 cut(s) 122, 154
FaqI GGGAC 2 cut(s) 129, 211
FatI CATG 6 cut(s) 152, 295, 381, 475, 811, 918
FauI CCCGC 1 cut(s) 694
FbaI TGATCA 1 cut(s) 739
FblI GTMKAC 2 cut(s) 333, 435
Fnu4HI GCNGC 1 cut(s) 27
FokI GGATG 4 cut(s) 89, 278, 440, 700
FriOI GRGCYC 2 cut(s) 138, 303
Fsp4HI GCNGC 1 cut(s) 27
FspBI CTAG 2 cut(s) 93, 779
GlaI GCGC 1 cut(s) 98
GluI GCNGC 1 cut(s) 27
HaeII RGCGCY 1 cut(s) 100
HaeIII GGCC 2 cut(s) 290, 765
HapII CCGG 2 cut(s) 831, 866
HhaI GCGC 1 cut(s) 99
Hin1I GRCGYC 1 cut(s) 97
Hin1II CATG 6 cut(s) 156, 299, 385, 479, 815, 922
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HindIII AAGCTT 1 cut(s) 839
HinfI GANTC 1 cut(s) 887
HpaII CCGG 2 cut(s) 831, 866
HphI GGTGA 5 cut(s) 26, 382, 398, 493, 862
Hpy166II GTNNAC 2 cut(s) 334, 436
Hpy188I TCNGA 5 cut(s) 105, 126, 603, 718, 982
Hpy188III TCNNGA 8 cut(s) 146, 166, 196, 322, 367, 457, 496, 743
Hpy8I GTNNAC 2 cut(s) 334, 436
HpyAV CCTTC 4 cut(s) 328, 527, 548, 847
HpyCH4III ACNGT 4 cut(s) 175, 341, 940, 948
HpyCH4IV ACGT 2 cut(s) 749, 789
HpyCH4V TGCA 4 cut(s) 221, 620, 691, 863
HpyF10VI GCNNNNNNNGC 1 cut(s) 636
HpyF3I CTNAG 3 cut(s) 114, 123, 302
HpySE526I ACGT 2 cut(s) 749, 789
Hsp92I GRCGYC 1 cut(s) 97
Hsp92II CATG 6 cut(s) 156, 299, 385, 479, 815, 922
HspAI GCGC 1 cut(s) 97
KasI GGCGCC 1 cut(s) 96
Ksp22I TGATCA 1 cut(s) 739
Kzo9I GATC 5 cut(s) 105, 660, 739, 912, 931
LguI GCTCTTC 1 cut(s) 143
LmnI GCTCC 4 cut(s) 133, 718, 834, 903
Lsp1109I GCAGC 1 cut(s) 38
LweI GCATC 1 cut(s) 678
MaeI CTAG 2 cut(s) 93, 779
MaeII ACGT 2 cut(s) 749, 789
MaeIII GTNAC 6 cut(s) 183, 305, 388, 481, 493, 704
MalI GATC 5 cut(s) 107, 662, 741, 914, 933
MboI GATC 5 cut(s) 105, 660, 739, 912, 931
MflI RGATCY 1 cut(s) 105
MhlI GDGCHC 4 cut(s) 138, 303, 417, 810
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 1 cut(s) 825
MluNI TGGCCA 1 cut(s) 290
Mly113I GGCGCC 1 cut(s) 97
MlyI GAGTC 1 cut(s) 896
MmeI TCCRAC 1 cut(s) 922
Mox20I TGGCCA 1 cut(s) 290
MscI TGGCCA 1 cut(s) 290
MseI TTAA 1 cut(s) 129
MslI CAYNNNNRTG 1 cut(s) 446
Msp20I TGGCCA 1 cut(s) 290
MspI CCGG 2 cut(s) 831, 866
MspR9I CCNGG 4 cut(s) 428, 570, 831, 832
MvaI CCWGG 2 cut(s) 428, 570
MwoI GCNNNNNNNGC 1 cut(s) 636
NarI GGCGCC 1 cut(s) 97
NciI CCSGG 2 cut(s) 831, 832
NcoI CCATGG 1 cut(s) 152
NdeII GATC 5 cut(s) 105, 660, 739, 912, 931
NlaIII CATG 6 cut(s) 156, 299, 385, 479, 815, 922
NlaIV GGNNCC 3 cut(s) 98, 145, 905
NmuCI GTSAC 5 cut(s) 305, 388, 481, 493, 704
NspI RCATGY 1 cut(s) 922
PciI ACATGT 1 cut(s) 918
PciSI GCTCTTC 1 cut(s) 143
PkrI GCNGC 1 cut(s) 28
PleI GAGTC 1 cut(s) 895
PluTI GGCGCC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 895
Ppu21I YACGTR 1 cut(s) 790
PscI ACATGT 1 cut(s) 918
PshAI GACNNNNGTC 1 cut(s) 329
Psp124BI GAGCTC 2 cut(s) 138, 303
Psp6I CCWGG 2 cut(s) 426, 568
PspGI CCWGG 2 cut(s) 426, 568
PspN4I GGNNCC 3 cut(s) 98, 145, 905
PspPI GGNCC 4 cut(s) 66, 143, 764, 944
PsuI RGATCY 1 cut(s) 105
RsaI GTAC 2 cut(s) 119, 923
RsaNI GTAC 2 cut(s) 118, 922
RseI CAYNNNNRTG 1 cut(s) 446
SacI GAGCTC 2 cut(s) 138, 303
SapI GCTCTTC 1 cut(s) 143
SaqAI TTAA 1 cut(s) 129
SatI GCNGC 1 cut(s) 27
Sau3AI GATC 5 cut(s) 105, 660, 739, 912, 931
Sau96I GGNCC 4 cut(s) 66, 143, 764, 944
SchI GAGTC 1 cut(s) 896
ScrFI CCNGG 4 cut(s) 428, 570, 831, 832
SduI GDGCHC 4 cut(s) 138, 303, 417, 810
SfaNI GCATC 1 cut(s) 678
SfcI CTRYAG 2 cut(s) 51, 204
SfoI GGCGCC 1 cut(s) 98
SinI GGWCC 3 cut(s) 66, 143, 944
SmaI CCCGGG 1 cut(s) 832
SmiMI CAYNNNNRTG 1 cut(s) 446
SmlI CTYRAG 2 cut(s) 164, 410
SmoI CTYRAG 2 cut(s) 164, 410
Sse9I AATT 1 cut(s) 825
SsiI CCGC 4 cut(s) 421, 681, 701, 929
SspDI GGCGCC 1 cut(s) 96
SspMI CTAG 2 cut(s) 93, 779
SstI GAGCTC 2 cut(s) 138, 303
StyD4I CCNGG 4 cut(s) 426, 568, 829, 830
StyI CCWWGG 1 cut(s) 152
TaaI ACNGT 4 cut(s) 175, 341, 940, 948
TaiI ACGT 2 cut(s) 752, 792
TaqI TCGA 4 cut(s) 323, 612, 934, 955
TaqII GACCGA 1 cut(s) 131
TasI AATT 1 cut(s) 825
TatI WGTACW 1 cut(s) 921
Tru1I TTAA 1 cut(s) 129
Tru9I TTAA 1 cut(s) 129
TseFI GTSAC 5 cut(s) 305, 388, 481, 493, 704
TseI GCWGC 1 cut(s) 26
Tsp45I GTSAC 5 cut(s) 305, 388, 481, 493, 704
TspDTI ATGAA 4 cut(s) 586, 700, 833, 974
TspGWI ACGGA 1 cut(s) 89
TspMI CCCGGG 1 cut(s) 830
VpaK11BI GGWCC 3 cut(s) 66, 143, 944
XapI RAATTY 1 cut(s) 825
XceI RCATGY 1 cut(s) 922
XmaI CCCGGG 1 cut(s) 830
XmiI GTMKAC 2 cut(s) 333, 435
XspI CTAG 2 cut(s) 93, 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.