pycom10g25390

glycine-rich cell wall structural protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010073
Physical Location & Seq
Reverse (-)
27048943 .. 27049407
465 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 465 bp
ATGATCCCAGCAACAAGTCTTTTCCTAGCCATTTTGCTCCTCACTGCTTCACTTGGCATCGCAGCCCGACCGGGATCCGATGGAGCCTTCTCAGCTGAGCTCAAGAACTCAAAGGACAAAGGTGGTGCTGCTAATGGTGGTGGCGGCGGGGGTGGCAACAGTGGTGGCATCGGGGGGTTTTTCCCGCCCGGGTTTGACATACCCGGGTTCGGAAAGGGTTTTGGAAGCGGTGTAGGTGGCGGGTATGGAGGCGGTTATGGGGGTCCGAATGGAGGGTACTCCAAGGGTGGAACTGTGAGGACTTCTCTTGTGTGTAAAGAAAAGGGTCCGTGCTACAAGAAGAAGCTGACGTGCCCTGCTAAGTGCTTCACATCCTACAGCCGGTCAGGGAAGGGTTATGGCGGCGGCGGCGGCGGCGGTGGGTGCACTATCGACTGCCAGAAGAAGTGTACTGCTTACTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

14.83

Weight (kDa)

9.41

Isoelectric Point (pI)

29.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61255 AT2G19000 AT4G21620 AT4G21620 AT5G60530
fragaria_vesca FvH4_3g04450
malus_domestica MD05G1323200.v1.1 MD10G1301000.v1.1
prunus_persica Prupe.4G039400_v2.0.a1
pyrus_communis pycom05g29750 pycom10g25390
rosa_chinensis RchiOBHm_Chr5g0006831
rosa_laevigata RLG00000031432
rosa_multiflora Rmu_co8368257.1_g000001 Rmu_sc0001044.1_g000036
rosa_roxburghii Rroxscaffold_1G00069220
rosa_rugosa Rorug04G0424800
rosa_samantha Rh5AG052500 Rh5BG051600 Rh5CG060100
rosa_wichuraiana Rw5G005210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 69, 82
AfaI GTAC 2 cut(s) 278, 451
AfiI CCNNNNNNNGG 3 cut(s) 209, 272, 381
AjiI CACGTC 1 cut(s) 351
AluBI AGCT 3 cut(s) 95, 100, 346
AluI AGCT 3 cut(s) 95, 100, 346
Alw21I GWGCWC 2 cut(s) 102, 428
Alw44I GTGCAC 1 cut(s) 424
AlwI GGATC 2 cut(s) 69, 82
Ama87I CYCGRG 2 cut(s) 188, 203
ApaLI GTGCAC 1 cut(s) 424
ApeKI GCWGC 2 cut(s) 62, 128
AspS9I GGNCC 2 cut(s) 263, 326
AsuC2I CCSGG 5 cut(s) 72, 189, 190, 204, 205
AvaI CYCGRG 2 cut(s) 188, 203
AvaII GGWCC 2 cut(s) 263, 326
BaeGI GKGCMC 2 cut(s) 356, 428
BamHI GGATCC 1 cut(s) 74
BanII GRGCYC 1 cut(s) 102
Bbv12I GWGCWC 2 cut(s) 102, 428
BbvI GCAGC 2 cut(s) 74, 115
BccI CCATC 1 cut(s) 74
BcnI CCSGG 5 cut(s) 72, 189, 190, 204, 205
BfaI CTAG 2 cut(s) 26, 463
BfmI CTRYAG 1 cut(s) 376
BisI GCNGC 8 cut(s) 63, 129, 145, 403, 406, 409, 412, 415
BlpI GCTNAGC 1 cut(s) 96
BlsI GCNGC 8 cut(s) 64, 130, 146, 404, 407, 410, 413, 416
Bme1390I CCNGG 5 cut(s) 72, 189, 190, 204, 205
Bme18I GGWCC 2 cut(s) 263, 326
BmeT110I CYCGRG 2 cut(s) 188, 203
BmgBI CACGTC 1 cut(s) 351
BmgT120I GGNCC 2 cut(s) 263, 326
BmiI GGNNCC 4 cut(s) 76, 85, 264, 327
BmrFI CCNGG 5 cut(s) 72, 189, 190, 204, 205
BmsI GCATC 2 cut(s) 66, 177
BplI GAGNNNNNCTC 2 cut(s) 289, 321
Bpu1102I GCTNAGC 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 5 cut(s) 72, 189, 190, 204, 205
BsaJI CCNNGG 3 cut(s) 188, 203, 282
Bsc4I CCNNNNNNNGG 3 cut(s) 209, 272, 381
Bse118I RCCGGY 1 cut(s) 381
BseDI CCNNGG 3 cut(s) 188, 203, 282
BseGI GGATG 1 cut(s) 371
BseLI CCNNNNNNNGG 3 cut(s) 209, 272, 381
BseMII CTCAG 2 cut(s) 87, 105
BseRI GAGGAG 1 cut(s) 29
BseSI GKGCMC 2 cut(s) 356, 428
BseXI GCAGC 2 cut(s) 74, 115
BseYI CCCAGC 1 cut(s) 7
Bsh1285I CGRYCG 1 cut(s) 71
BsiEI CGRYCG 1 cut(s) 71
BsiHKAI GWGCWC 2 cut(s) 102, 428
BsiHKCI CYCGRG 2 cut(s) 188, 203
BsiSI CCGG 4 cut(s) 71, 189, 204, 382
BslI CCNNNNNNNGG 3 cut(s) 209, 272, 381
BsoBI CYCGRG 2 cut(s) 188, 203
Bsp1286I GDGCHC 3 cut(s) 102, 356, 428
Bsp143I GATC 2 cut(s) 3, 74
Bsp1720I GCTNAGC 1 cut(s) 96
BspCNI CTCAG 2 cut(s) 88, 104
BspLI GGNNCC 4 cut(s) 76, 85, 264, 327
BspPI GGATC 2 cut(s) 69, 82
BsrFI RCCGGY 1 cut(s) 381
BssAI RCCGGY 1 cut(s) 381
BssECI CCNNGG 3 cut(s) 188, 203, 282
BssMI GATC 2 cut(s) 3, 74
BssT1I CCWWGG 1 cut(s) 282
Bst4CI ACNGT 2 cut(s) 161, 295
BstDEI CTNAG 3 cut(s) 91, 96, 360
BstF5I GGATG 1 cut(s) 371
BstKTI GATC 2 cut(s) 6, 77
BstMBI GATC 2 cut(s) 3, 74
BstMCI CGRYCG 1 cut(s) 71
BstMWI GCNNNNNNNGC 6 cut(s) 92, 153, 408, 411, 414, 423
BstSCI CCNGG 5 cut(s) 70, 187, 188, 202, 203
BstSFI CTRYAG 1 cut(s) 376
BstSLI GKGCMC 2 cut(s) 356, 428
BstV1I GCAGC 2 cut(s) 74, 115
BstX2I RGATCY 1 cut(s) 74
BstYI RGATCY 1 cut(s) 74
BtgZI GCGATG 1 cut(s) 43
BtrI CACGTC 1 cut(s) 351
BtsCI GGATG 1 cut(s) 371
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 2 cut(s) 42, 166
Cfr10I RCCGGY 1 cut(s) 381
Cfr13I GGNCC 2 cut(s) 263, 326
Cfr9I CCCGGG 2 cut(s) 188, 203
Csp6I GTAC 2 cut(s) 277, 450
CspCI CAANNNNNGTGG 2 cut(s) 145, 180
CviJI RGCY 7 cut(s) 29, 65, 86, 95, 100, 346, 381
CviKI_1 RGCY 7 cut(s) 29, 65, 86, 95, 100, 346, 381
CviQI GTAC 2 cut(s) 277, 450
DdeI CTNAG 3 cut(s) 91, 96, 360
DpnI GATC 2 cut(s) 5, 76
DpnII GATC 2 cut(s) 3, 74
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 282
Eco24I GRGCYC 1 cut(s) 102
Eco47I GGWCC 2 cut(s) 263, 326
Eco53kI GAGCTC 1 cut(s) 100
Eco88I CYCGRG 2 cut(s) 188, 203
EcoICRI GAGCTC 1 cut(s) 100
EcoT14I CCWWGG 1 cut(s) 282
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 282
FaiI YATR 4 cut(s) 200, 246, 258, 399
FauI CCCGC 3 cut(s) 140, 192, 233
Fnu4HI GCNGC 8 cut(s) 63, 129, 145, 403, 406, 409, 412, 415
FokI GGATG 1 cut(s) 358
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 8 cut(s) 63, 129, 145, 403, 406, 409, 412, 415
FspBI CTAG 2 cut(s) 26, 463
GluI GCNGC 8 cut(s) 63, 129, 145, 403, 406, 409, 412, 415
GsaI CCCAGC 1 cut(s) 11
HapII CCGG 4 cut(s) 71, 189, 204, 382
HpaII CCGG 4 cut(s) 71, 189, 204, 382
Hpy166II GTNNAC 2 cut(s) 426, 450
Hpy188I TCNGA 3 cut(s) 79, 212, 267
Hpy188III TCNNGA 1 cut(s) 103
Hpy8I GTNNAC 2 cut(s) 426, 450
HpyAV CCTTC 2 cut(s) 97, 385
HpyCH4III ACNGT 2 cut(s) 161, 295
HpyCH4IV ACGT 1 cut(s) 350
HpyCH4V TGCA 1 cut(s) 426
HpyF10VI GCNNNNNNNGC 6 cut(s) 92, 153, 408, 411, 414, 423
HpyF3I CTNAG 3 cut(s) 91, 96, 360
HpySE526I ACGT 1 cut(s) 350
Kzo9I GATC 2 cut(s) 3, 74
LmnI GCTCC 2 cut(s) 42, 83
LpnPI CCDG 8 cut(s) 21, 84, 202, 217, 369, 372, 395, 452
Lsp1109I GCAGC 2 cut(s) 74, 115
LweI GCATC 2 cut(s) 66, 177
MaeI CTAG 2 cut(s) 26, 463
MaeII ACGT 1 cut(s) 350
MalI GATC 2 cut(s) 5, 76
MboI GATC 2 cut(s) 3, 74
MboII GAAGA 2 cut(s) 352, 454
MflI RGATCY 1 cut(s) 74
MhlI GDGCHC 3 cut(s) 102, 356, 428
MnlI CCTC 4 cut(s) 50, 242, 266, 291
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 4 cut(s) 71, 189, 204, 382
MspR9I CCNGG 5 cut(s) 72, 189, 190, 204, 205
MwoI GCNNNNNNNGC 6 cut(s) 92, 153, 408, 411, 414, 423
NciI CCSGG 5 cut(s) 72, 189, 190, 204, 205
NdeII GATC 2 cut(s) 3, 74
NlaIV GGNNCC 4 cut(s) 76, 85, 264, 327
PkrI GCNGC 8 cut(s) 64, 130, 146, 404, 407, 410, 413, 416
Psp124BI GAGCTC 1 cut(s) 102
PspFI CCCAGC 1 cut(s) 7
PspN4I GGNNCC 4 cut(s) 76, 85, 264, 327
PspPI GGNCC 2 cut(s) 263, 326
PsuI RGATCY 1 cut(s) 74
PvuII CAGCTG 1 cut(s) 95
RsaI GTAC 2 cut(s) 278, 451
RsaNI GTAC 2 cut(s) 277, 450
SacI GAGCTC 1 cut(s) 102
SatI GCNGC 8 cut(s) 63, 129, 145, 403, 406, 409, 412, 415
Sau3AI GATC 2 cut(s) 3, 74
Sau96I GGNCC 2 cut(s) 263, 326
ScrFI CCNGG 5 cut(s) 72, 189, 190, 204, 205
SduI GDGCHC 3 cut(s) 102, 356, 428
SetI ASST 6 cut(s) 97, 102, 124, 238, 348, 353
SfaNI GCATC 2 cut(s) 66, 177
SfcI CTRYAG 1 cut(s) 376
SinI GGWCC 2 cut(s) 263, 326
SmaI CCCGGG 2 cut(s) 190, 205
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
SspMI CTAG 2 cut(s) 26, 463
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 5 cut(s) 70, 187, 188, 202, 203
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 2 cut(s) 161, 295
TaiI ACGT 1 cut(s) 353
TaqI TCGA 1 cut(s) 432
TatI WGTACW 1 cut(s) 449
TauI GCSGC 6 cut(s) 147, 405, 408, 411, 414, 417
TscAI CASTG 2 cut(s) 49, 166
TseI GCWGC 2 cut(s) 62, 128
TspGWI ACGGA 1 cut(s) 318
TspMI CCCGGG 2 cut(s) 188, 203
TspRI CASTG 2 cut(s) 49, 166
VneI GTGCAC 1 cut(s) 424
VpaK11BI GGWCC 2 cut(s) 263, 326
XmaI CCCGGG 2 cut(s) 188, 203
XspI CTAG 2 cut(s) 26, 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.