Rorug04G0424800

glycine-rich cell wall structural protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010073
Physical Location & Seq
Forward (+)
58409946 .. 58410851
906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0424800.1

Sequence Viewer

Length: 906 bp
ATGTCTTCAGTCCCTGTACTAAATTTCGTCGAGGATGATGAGTTAGACCGCCACAACCTTAATAGTAGCGATTATCTACGTCAACACAACATTTTCATCAACTTCTTGCAAGGGTATGTAGAATATCGTGACAACACAAAGCTCATAGACAAACTAAGGCTTCACATGAGGATGTTCACGTGTCAAGATGCTGCAACCATTGATAGTTGGTTAGGACTTGCTATAGAGGGAGATGTGAAAGAGTTAGATATCAGCCCTAAAATGAGTGTTGACCCGAAACAATATAGAGAGGATGCGCTCAGTGAGCGCTGGGTGGAGAGATACGACCACTACTGCTGGTACCGCATACCTTGGGTGATCATTGTTAATGCCAAATCTTTAACTAGTCTAAATCTGGAGAGTATGAGAGTTCCGGACATTCGAGCTGAGGTAGACATTGAACGTGACCACCCCCTATTCCCCTTTTTGAAAATCATGTCCTTCACAGCTGTGACGTTCCATGGCTATAGTTTCTTCCCTGTACTATGGGAGTCCTCTTCCATCGAGTACTTGTCATTAACTGCATGTTCTTTTGAGAACCATGACCCTATGTGTTACGTTAAAAGTTCGAGTCTCAAGTGCTTGGAAGTTAAGTATTGTCATGGGCTTAAGTTTATTATTGTTCACGAAGCTATGAATCTCGAATCTCTTACGTTTGTTTCACCGCCACCACCATATTCAAAATGTATAAGGGTGCCGGTGAATAATACACCCAACTTGAGAAAGATCGATATTTGTGTTGATGAACTCGGGGAATTATATTTATGGCAATGCCATCGTGCACTAAAGGCCACCATCAATACCCAAAATCTCAATCAATTCAGATTCTATGGTTCATTGGAAGCCCAAGTTTCTGTTAAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

301

Amino Acids

35.18

Weight (kDa)

5.7

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 121 - 264 1.7e-09 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61255 AT2G19000 AT4G21620 AT4G21620 AT5G60530
fragaria_vesca FvH4_3g04450
malus_domestica MD05G1323200.v1.1 MD10G1301000.v1.1
prunus_persica Prupe.4G039400_v2.0.a1
pyrus_communis pycom05g29750 pycom10g25390
rosa_chinensis RchiOBHm_Chr5g0006831
rosa_laevigata RLG00000031432
rosa_multiflora Rmu_co8368257.1_g000001 Rmu_sc0001044.1_g000036
rosa_roxburghii Rroxscaffold_1G00069220
rosa_rugosa Rorug04G0424800
rosa_samantha Rh5AG052500 Rh5BG051600 Rh5CG060100
rosa_wichuraiana Rw5G005210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 339
AccB1I GGYRCC 2 cut(s) 339, 733
AccI GTMKAC 1 cut(s) 432
AccIII TCCGGA 1 cut(s) 412
AciI CCGC 3 cut(s) 49, 343, 704
AcsI RAATTY 1 cut(s) 22
AcvI CACGTG 1 cut(s) 180
AfaI GTAC 4 cut(s) 18, 341, 522, 548
AfeI AGCGCT 1 cut(s) 308
AflII CTTAAG 1 cut(s) 647
AflIII ACRYGT 1 cut(s) 179
AgsI TTSAA 3 cut(s) 440, 469, 720
AhlI ACTAGT 1 cut(s) 383
AleI CACNNNNGTG 1 cut(s) 488
AluBI AGCT 4 cut(s) 142, 425, 488, 671
AluI AGCT 4 cut(s) 142, 425, 488, 671
Alw21I GWGCWC 1 cut(s) 823
Alw26I GTCTC 1 cut(s) 617
Alw44I GTGCAC 1 cut(s) 819
AlwNI CAGNNNCTG 1 cut(s) 14
Ama87I CYCGRG 1 cut(s) 788
Aor13HI TCCGGA 1 cut(s) 412
Aor51HI AGCGCT 1 cut(s) 308
AoxI GGCC 1 cut(s) 828
ApaLI GTGCAC 1 cut(s) 819
ApeKI GCWGC 1 cut(s) 191
ApoI RAATTY 1 cut(s) 22
Asp718I GGTACC 1 cut(s) 339
AspLEI GCGC 2 cut(s) 298, 309
AsuHPI GGTGA 3 cut(s) 367, 693, 751
AvaI CYCGRG 1 cut(s) 788
BaeGI GKGCMC 1 cut(s) 823
BanI GGYRCC 2 cut(s) 339, 733
BbrPI CACGTG 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 823
BbvCI CCTCAGC 1 cut(s) 426
BbvI GCAGC 1 cut(s) 178
BccI CCATC 3 cut(s) 548, 822, 842
BcgI CGANNNNNNTGC 2 cut(s) 797, 831
BclI TGATCA 1 cut(s) 357
BcoDI GTCTC 1 cut(s) 617
BcuI ACTAGT 1 cut(s) 383
BfaI CTAG 1 cut(s) 384
BfmI CTRYAG 2 cut(s) 222, 505
BfoI RGCGCY 1 cut(s) 310
BfrI CTTAAG 1 cut(s) 647
BisI GCNGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 193
BmcAI AGTACT 1 cut(s) 548
BmeT110I CYCGRG 1 cut(s) 788
BmiI GGNNCC 2 cut(s) 341, 735
BmsI GCATC 2 cut(s) 178, 283
BpmI CTGGAG 1 cut(s) 416
Bpu10I CCTNAGC 1 cut(s) 426
BpuEI CTTGAG 2 cut(s) 599, 778
Bsa29I ATCGAT 1 cut(s) 768
BsaAI YACGTR 1 cut(s) 180
BsaJI CCNNGG 2 cut(s) 350, 499
BsaWI WCCGGW 1 cut(s) 412
Bse118I RCCGGY 1 cut(s) 736
Bse3DI GCAATG 1 cut(s) 815
BseAI TCCGGA 1 cut(s) 412
BseCI ATCGAT 1 cut(s) 768
BseDI CCNNGG 2 cut(s) 350, 499
BseGI GGATG 3 cut(s) 40, 177, 298
BseMI GCAATG 1 cut(s) 815
BseMII CTCAG 2 cut(s) 313, 417
BseSI GKGCMC 1 cut(s) 823
BseXI GCAGC 1 cut(s) 178
BseYI CCCAGC 1 cut(s) 309
BshFI GGCC 1 cut(s) 830
BshNI GGYRCC 2 cut(s) 339, 733
BshVI ATCGAT 1 cut(s) 768
BsiHKAI GWGCWC 1 cut(s) 823
BsiHKCI CYCGRG 1 cut(s) 788
BsiSI CCGG 2 cut(s) 413, 737
BsmAI GTCTC 1 cut(s) 617
BsnI GGCC 1 cut(s) 830
BsoBI CYCGRG 1 cut(s) 788
Bsp1286I GDGCHC 1 cut(s) 823
Bsp13I TCCGGA 1 cut(s) 412
Bsp143I GATC 2 cut(s) 357, 765
Bsp19I CCATGG 1 cut(s) 499
BspACI CCGC 3 cut(s) 49, 343, 704
BspANI GGCC 1 cut(s) 830
BspCNI CTCAG 2 cut(s) 312, 418
BspDI ATCGAT 1 cut(s) 768
BspEI TCCGGA 1 cut(s) 412
BspLI GGNNCC 2 cut(s) 341, 735
BspT107I GGYRCC 2 cut(s) 339, 733
BspTI CTTAAG 1 cut(s) 647
BsrDI GCAATG 1 cut(s) 815
BsrFI RCCGGY 1 cut(s) 736
BssAI RCCGGY 1 cut(s) 736
BssECI CCNNGG 2 cut(s) 350, 499
BssMI GATC 2 cut(s) 357, 765
BssT1I CCWWGG 2 cut(s) 350, 499
Bst4CI ACNGT 1 cut(s) 903
Bst6I CTCTTC 1 cut(s) 541
BstAFI CTTAAG 1 cut(s) 647
BstBAI YACGTR 1 cut(s) 180
BstDEI CTNAG 3 cut(s) 155, 299, 426
BstDSI CCRYGG 1 cut(s) 499
BstF5I GGATG 3 cut(s) 40, 177, 298
BstH2I RGCGCY 1 cut(s) 310
BstHHI GCGC 2 cut(s) 298, 309
BstKTI GATC 2 cut(s) 360, 768
BstMAI GTCTC 1 cut(s) 617
BstMBI GATC 2 cut(s) 357, 765
BstMWI GCNNNNNNNGC 3 cut(s) 304, 342, 827
BstNSI RCATGY 1 cut(s) 567
BstSFI CTRYAG 2 cut(s) 222, 505
BstSLI GKGCMC 1 cut(s) 823
BstV1I GCAGC 1 cut(s) 178
Bsu15I ATCGAT 1 cut(s) 768
BsuRI GGCC 1 cut(s) 830
BsuTUI ATCGAT 1 cut(s) 768
BtgI CCRYGG 1 cut(s) 499
BtsCI GGATG 3 cut(s) 40, 177, 298
BtsIMutI CAGTG 1 cut(s) 307
CaiI CAGNNNCTG 1 cut(s) 14
CfoI GCGC 2 cut(s) 298, 309
Cfr10I RCCGGY 1 cut(s) 736
ClaI ATCGAT 1 cut(s) 768
Csp6I GTAC 4 cut(s) 17, 340, 521, 547
CviAII CATG 6 cut(s) 166, 475, 500, 564, 581, 641
CviQI GTAC 4 cut(s) 17, 340, 521, 547
DdeI CTNAG 3 cut(s) 155, 299, 426
DpnI GATC 2 cut(s) 359, 767
DpnII GATC 2 cut(s) 357, 765
Eam1104I CTCTTC 1 cut(s) 541
EarI CTCTTC 1 cut(s) 541
Eco130I CCWWGG 2 cut(s) 350, 499
Eco32I GATATC 1 cut(s) 250
Eco47III AGCGCT 1 cut(s) 308
Eco72I CACGTG 1 cut(s) 180
Eco88I CYCGRG 1 cut(s) 788
EcoRV GATATC 1 cut(s) 250
EcoT14I CCWWGG 2 cut(s) 350, 499
ErhI CCWWGG 2 cut(s) 350, 499
FaeI CATG 6 cut(s) 169, 478, 503, 567, 584, 644
FatI CATG 6 cut(s) 165, 474, 499, 563, 580, 640
FbaI TGATCA 1 cut(s) 357
FblI GTMKAC 1 cut(s) 432
Fnu4HI GCNGC 1 cut(s) 192
FokI GGATG 3 cut(s) 47, 184, 305
Fsp4HI GCNGC 1 cut(s) 192
FspBI CTAG 1 cut(s) 384
GlaI GCGC 2 cut(s) 297, 308
GluI GCNGC 1 cut(s) 192
GsaI CCCAGC 1 cut(s) 313
GsuI CTGGAG 1 cut(s) 416
HaeII RGCGCY 1 cut(s) 310
HaeIII GGCC 1 cut(s) 830
HapII CCGG 2 cut(s) 413, 737
HhaI GCGC 2 cut(s) 298, 309
Hin1II CATG 6 cut(s) 169, 478, 503, 567, 584, 644
Hin6I GCGC 2 cut(s) 296, 307
HinP1I GCGC 2 cut(s) 296, 307
HincII GTYRAC 2 cut(s) 83, 271
HindII GTYRAC 2 cut(s) 83, 271
HinfI GANTC 5 cut(s) 530, 610, 676, 683, 864
HpaII CCGG 2 cut(s) 413, 737
HphI GGTGA 3 cut(s) 367, 693, 751
Hpy166II GTNNAC 6 cut(s) 83, 177, 271, 433, 664, 821
Hpy188I TCNGA 1 cut(s) 863
Hpy188III TCNNGA 6 cut(s) 128, 185, 395, 413, 665, 680
Hpy8I GTNNAC 6 cut(s) 83, 177, 271, 433, 664, 821
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 1 cut(s) 490
HpyCH4III ACNGT 1 cut(s) 903
HpyCH4IV ACGT 6 cut(s) 79, 179, 442, 494, 597, 692
HpyCH4V TGCA 4 cut(s) 109, 194, 563, 821
HpyF10VI GCNNNNNNNGC 3 cut(s) 304, 342, 827
HpyF3I CTNAG 3 cut(s) 155, 299, 426
HpySE526I ACGT 6 cut(s) 79, 179, 442, 494, 597, 692
Hsp92II CATG 6 cut(s) 169, 478, 503, 567, 584, 644
HspAI GCGC 2 cut(s) 296, 307
Kpn2I TCCGGA 1 cut(s) 412
KpnI GGTACC 1 cut(s) 343
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 2 cut(s) 357, 765
LpnPI CCDG 7 cut(s) 27, 295, 322, 380, 426, 531, 750
Lsp1109I GCAGC 1 cut(s) 178
LweI GCATC 2 cut(s) 178, 283
MaeI CTAG 1 cut(s) 384
MaeII ACGT 6 cut(s) 79, 179, 442, 494, 597, 692
MaeIII GTNAC 4 cut(s) 128, 443, 490, 593
MalI GATC 2 cut(s) 359, 767
MboI GATC 2 cut(s) 357, 765
MboII GAAGA 2 cut(s) 505, 528
MhlI GDGCHC 1 cut(s) 823
MluCI AATT 3 cut(s) 22, 794, 857
MlyI GAGTC 2 cut(s) 539, 619
MnlI CCTC 6 cut(s) 25, 162, 220, 283, 421, 544
MroI TCCGGA 1 cut(s) 412
MseI TTAA 8 cut(s) 60, 366, 380, 557, 600, 630, 648, 897
MslI CAYNNNNRTG 2 cut(s) 170, 488
MspA1I CMGCKG 1 cut(s) 488
MspCI CTTAAG 1 cut(s) 647
MspI CCGG 2 cut(s) 413, 737
MwoI GCNNNNNNNGC 3 cut(s) 304, 342, 827
NcoI CCATGG 1 cut(s) 499
NdeII GATC 2 cut(s) 357, 765
NlaIII CATG 6 cut(s) 169, 478, 503, 567, 584, 644
NlaIV GGNNCC 2 cut(s) 341, 735
NmuCI GTSAC 3 cut(s) 128, 443, 490
NspI RCATGY 1 cut(s) 567
OliI CACNNNNGTG 1 cut(s) 488
PfeI GAWTC 3 cut(s) 676, 683, 864
PkrI GCNGC 1 cut(s) 193
PleI GAGTC 2 cut(s) 538, 618
PmaCI CACGTG 1 cut(s) 180
PmlI CACGTG 1 cut(s) 180
PpsI GAGTC 2 cut(s) 538, 618
Ppu21I YACGTR 1 cut(s) 180
PspCI CACGTG 1 cut(s) 180
PspFI CCCAGC 1 cut(s) 309
PspN4I GGNNCC 2 cut(s) 341, 735
PstNI CAGNNNCTG 1 cut(s) 14
PvuII CAGCTG 1 cut(s) 488
RsaI GTAC 4 cut(s) 18, 341, 522, 548
RsaNI GTAC 4 cut(s) 17, 340, 521, 547
RseI CAYNNNNRTG 2 cut(s) 170, 488
SaqAI TTAA 8 cut(s) 60, 366, 380, 557, 600, 630, 648, 897
SatI GCNGC 1 cut(s) 192
Sau3AI GATC 2 cut(s) 357, 765
ScaI AGTACT 1 cut(s) 548
SchI GAGTC 2 cut(s) 539, 619
SduI GDGCHC 1 cut(s) 823
SfaNI GCATC 2 cut(s) 178, 283
SfcI CTRYAG 2 cut(s) 222, 505
SmiMI CAYNNNNRTG 2 cut(s) 170, 488
SmlI CTYRAG 3 cut(s) 614, 647, 757
SmoI CTYRAG 3 cut(s) 614, 647, 757
SpeI ACTAGT 1 cut(s) 383
Sse9I AATT 3 cut(s) 22, 794, 857
SsiI CCGC 3 cut(s) 49, 343, 704
SspMI CTAG 1 cut(s) 384
StyI CCWWGG 2 cut(s) 350, 499
TaaI ACNGT 1 cut(s) 903
TaiI ACGT 6 cut(s) 82, 182, 445, 497, 600, 695
TaqI TCGA 6 cut(s) 30, 421, 543, 608, 681, 768
TasI AATT 3 cut(s) 22, 794, 857
TatI WGTACW 3 cut(s) 16, 520, 546
TfiI GAWTC 3 cut(s) 676, 683, 864
Tru1I TTAA 8 cut(s) 60, 366, 380, 557, 600, 630, 648, 897
Tru9I TTAA 8 cut(s) 60, 366, 380, 557, 600, 630, 648, 897
TscAI CASTG 1 cut(s) 307
TseFI GTSAC 3 cut(s) 128, 443, 490
TseI GCWGC 1 cut(s) 191
Tsp45I GTSAC 3 cut(s) 128, 443, 490
TspDTI ATGAA 4 cut(s) 85, 689, 798, 864
TspRI CASTG 1 cut(s) 307
Vha464I CTTAAG 1 cut(s) 647
VneI GTGCAC 1 cut(s) 819
XapI RAATTY 1 cut(s) 22
XceI RCATGY 1 cut(s) 567
XmiI GTMKAC 1 cut(s) 432
XspI CTAG 1 cut(s) 384
ZrmI AGTACT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.