Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0007972

Physical Location & Seq

Genomic Coordinates Forward (+)

27154099 .. 27158106

Gene Structure 4008 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1296 bp

ATGGGAGAAGAAGAAGAGCCCCAGCAGCAGCAGCAGCAGCACCAACCCAATTCGCCTTCCCCAGACGCAGAAATTACAGAAAACCCAACCCAGCAACAGCAAGAATACTCTTGGCCGGCGATTCGGTTCGATGTCCATCCGCAGAGGGCCTTCCACTTCCGCCGCCAGTTCCGAACTTCCCCAATCCCCAACAACTTTCTCAAGGGCGTCAAATGGTCGCCGGATGGGTCGTGCTTTCTTACCAGTTCCGAGGACAACACGCTTCGGGTTTTTGCATTGCCAGATTATGGGAGTGAGAATGCTAATGGGTGTTCTGTAGCTGCTGATGAAGATTCGTATGCTGCAAACCTTGTTGTGAATGAGGGAGAGTCTGTATACGACTTCTGTTGGTACCCGTACATGTCTGCTTCAGACCCGGTTACCTGTGTTTTTGCAACTACATCTCGTGACCATCCAATTCATCTTTGGGATGCTTCTTCTGGACAGCTACGTTGCACTTATCGTGCTTATGATGCCGTGGATGAAATCACAGCTGCTTTTTCAATTGCCTTTAATCCTACTGGAACAAAGATTTTTGCCGGATACAACAAATCTGTTCGGGTGTTTGATTTACATCGCCCTGGTAGGGATTTTGATCAATATTCTACACTTCAAGGAAACAAAGAAGGCCAAACTGGTATAATGTCTGCAATGGCATTTTGTCCAACCCATAGTGGAATGCTAGCTATAGGTTCTTATAGCAAGACTGCTGCAATATATAGAGAAGATAATATGGAACCCTTGTATGTATTGCATGGTCATGAAGGTGGGATTACACATGTCCAGTTTTCGAAAGATGGAAATTATTTATATACTGGAGGCCGAAAGGATCCTTATATATTATGCTGGGATATACGCAAAGCGGTTGATGTTGTGTATAAGTTATATAGGTCATCGGAAGATACCAACCAACGGATTTTGTTTGATATTGAGCCACTTGGACGCCATCTTTGTACAGGCGGTCAGGATGGTGTTGTGCATATCTATGATCTGCAAACTGGACAATGGGTAACAGGCTTCCAAGCGGCATTGGATACCGTTAACGGATTCTCCTTTCATCCCTTTCTGCCATTGGCTACCACTTCTTCAGGGCATCGAAGATTTGTTGGTCCTGATGATGGCAATGAGGAATTGCATCTGAGTGGTGACGAAAACTGCGCTTCTGTGTGGAGTTTCTCTGTTGCTTCAATGGTGGAGAATGATGCTGCAATGGACACCGATGATTATAACAGCCACAACCTCGACCAGCATCCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005697 GO:0006996 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0015030 GO:0016043 GO:0016604 GO:0019219 GO:0019222 GO:0022607 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032200 GO:0032202 GO:0032203 GO:0032991 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051276 GO:0051338 GO:0051347 GO:0051972 GO:0051973 GO:0060255 GO:0065007 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0097159 GO:1901363 GO:1990904 GO:2000112 GO:2000278 GO:2000573

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

432

Amino Acids

48.28

Weight (kDa)

4.85

Isoelectric Point (pI)

45.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	68 - 367	3.3e-25	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	69 - 205	3.6e-06	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	137 - 301	6.7e-14	Echinoderm microtubule-associated protein second beta-propeller
EIF3I	PF24805	139 - 298	8.6e-06	EIF3I
WD40_Prp19	PF24814	142 - 365	1.1e-14	Prp19 WD40 domain
Beta-prop_EML	PF23409	143 - 298	6.5e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40_WDHD1_1st	PF24817	145 - 304	3.6e-08	WDHD1 first WD40 domain
Beta-prop_TEP1_2nd	PF25047	211 - 356	1.3e-11	TEP-1 second beta-propeller
Beta-prop_WDR3_1st	PF25173	214 - 367	6.7e-13	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	223 - 376	1.5e-14	WDR5 beta-propeller domain
WDR55	PF24796	230 - 365	2e-08	WDR55
WD40	PF00400	259 - 297	3.8e-07	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21520 AT4G21520
fragaria_vesca	FvH4_3g04030 FvH4_3g04030 FvH4_3g04030 FvH4_3g04030 FvH4_3g04030 FvH4_3g04030
malus_domestica	MD05G1325300.v1.1 MD10G1303200.v1.1
prunus_persica	Prupe.4G036600_v2.0.a1
pyrus_communis	pycom05g29930 pycom10g25570
rosa_chinensis	RchiOBHm_Chr5g0006371
rosa_laevigata	RLG00000031362
rosa_multiflora	Rmu_sc0037704.1_g000001
rosa_roxburghii	Rroxscaffold_1G00069560
rosa_rugosa	Rorug04G0421200 Rorug04G0421200
rosa_samantha	Rh5AG049200 Rh5BG048500 Rh5CG056700 Rh5DG047800
rosa_wichuraiana	Rw5G004900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1266
Acc65I	GGTACC	1 cut(s)	390
AccB1I	GGYRCC	1 cut(s)	390
AccB7I	CCANNNNNTGG	1 cut(s)	287
AccI	GTMKAC	1 cut(s)	375
AciI	CCGC	6 cut(s)	140, 160, 163, 902, 999, 1064
AclWI	GGATC	2 cut(s)	863, 876
AcoI	YGGCCR	1 cut(s)	113
AcuI	CTGAAG	2 cut(s)	393, 1110
AcyI	GRCGYC	2 cut(s)	207, 982
AfaI	GTAC	3 cut(s)	392, 398, 994
AfiI	CCNNNNNNNGG	4 cut(s)	287, 625, 951, 1157
AflIII	ACRYGT	2 cut(s)	399, 817
AgsI	TTSAA	3 cut(s)	543, 653, 1227
AjnI	CCWGG	1 cut(s)	619
AluBI	AGCT	4 cut(s)	320, 487, 533, 725
AluI	AGCT	4 cut(s)	320, 487, 533, 725
AlwI	GGATC	2 cut(s)	863, 876
AoxI	GGCC	4 cut(s)	113, 147, 667, 859
ArsI	GACNNNNNNTTYG	2 cut(s)	972, 1004
Asp718I	GGTACC	1 cut(s)	390
AspLEI	GCGC	1 cut(s)	1199
AspS9I	GGNCC	2 cut(s)	147, 1148
AsuC2I	CCSGG	1 cut(s)	416
AsuHPI	GGTGA	1 cut(s)	1196
AsuII	TTCGAA	1 cut(s)	830
AsuNHI	GCTAGC	1 cut(s)	721
AvaII	GGWCC	1 cut(s)	1148
BamHI	GGATCC	1 cut(s)	868
BanI	GGYRCC	1 cut(s)	390
BanII	GRGCYC	1 cut(s)	21
BauI	CACGAG	1 cut(s)	444
BccI	CCATC	7 cut(s)	144, 218, 459, 830, 993, 1001, 1151
BceAI	ACGGC	1 cut(s)	500
BciT130I	CCWGG	1 cut(s)	621
BciVI	GTATCC	2 cut(s)	575, 1066
BclI	TGATCA	1 cut(s)	634
BcnI	CCSGG	1 cut(s)	416
BfaI	CTAG	2 cut(s)	722, 1294
BfmI	CTRYAG	2 cut(s)	315, 726
BfuI	GTATCC	2 cut(s)	575, 1066
Bme1390I	CCNGG	2 cut(s)	416, 621
Bme18I	GGWCC	1 cut(s)	1148
BmgT120I	GGNCC	2 cut(s)	147, 1148
BmiI	GGNNCC	3 cut(s)	392, 777, 870
BmrFI	CCNGG	2 cut(s)	416, 621
BmsI	GCATC	5 cut(s)	460, 502, 1141, 1183, 1231
BmtI	GCTAGC	1 cut(s)	725
BpmI	CTGGAG	1 cut(s)	876
Bpu14I	TTCGAA	1 cut(s)	830
BpuEI	CTTGAG	1 cut(s)	185
BpuMI	CCSGG	1 cut(s)	416
BsaBI	GATNNNNATC	3 cut(s)	135, 612, 633
BsaHI	GRCGYC	2 cut(s)	207, 982
BsaJI	CCNNGG	3 cut(s)	249, 516, 619
BsaXI	ACNNNNNCTCC	4 cut(s)	357, 387, 1073, 1103
Bsc4I	CCNNNNNNNGG	4 cut(s)	287, 625, 951, 1157
Bse118I	RCCGGY	1 cut(s)	115
Bse1I	ACTGG	7 cut(s)	166, 243, 565, 679, 823, 859, 1042
Bse3DI	GCAATG	4 cut(s)	275, 696, 1168, 1254
Bse8I	GATNNNNATC	3 cut(s)	135, 612, 633
BseBI	CCWGG	1 cut(s)	621
BseDI	CCNNGG	3 cut(s)	249, 516, 619
BseGI	GGATG	8 cut(s)	136, 229, 451, 475, 526, 1012, 1096, 1288
BseJI	GATNNNNATC	3 cut(s)	135, 612, 633
BseLI	CCNNNNNNNGG	4 cut(s)	287, 625, 951, 1157
BseMI	GCAATG	4 cut(s)	275, 696, 1168, 1254
BseMII	CTCAG	1 cut(s)	1169
BseNI	ACTGG	7 cut(s)	166, 243, 565, 679, 823, 859, 1042
BseYI	CCCAGC	3 cut(s)	21, 90, 885
BshFI	GGCC	4 cut(s)	115, 149, 669, 861
BshNI	GGYRCC	1 cut(s)	390
BsiSI	CCGG	4 cut(s)	116, 221, 416, 579
BslI	CCNNNNNNNGG	4 cut(s)	287, 625, 951, 1157
BsmI	GAATGC	2 cut(s)	304, 723
BsnI	GGCC	4 cut(s)	115, 149, 669, 861
Bsp119I	TTCGAA	1 cut(s)	830
Bsp1286I	GDGCHC	1 cut(s)	21
Bsp1407I	TGTACA	1 cut(s)	992
Bsp143I	GATC	3 cut(s)	634, 868, 1027
BspACI	CCGC	6 cut(s)	140, 160, 163, 902, 999, 1064
BspANI	GGCC	4 cut(s)	115, 149, 669, 861
BspCNI	CTCAG	1 cut(s)	1170
BspHI	TCATGA	1 cut(s)	799
BspLI	GGNNCC	3 cut(s)	392, 777, 870
BspOI	GCTAGC	1 cut(s)	725
BspPI	GGATC	2 cut(s)	863, 876
BspQI	GCTCTTC	1 cut(s)	9
BspT104I	TTCGAA	1 cut(s)	830
BspT107I	GGYRCC	1 cut(s)	390
BsrDI	GCAATG	4 cut(s)	275, 696, 1168, 1254
BsrFI	RCCGGY	1 cut(s)	115
BsrGI	TGTACA	1 cut(s)	992
BsrI	ACTGG	7 cut(s)	166, 243, 565, 679, 823, 859, 1042
BssAI	RCCGGY	1 cut(s)	115
BssECI	CCNNGG	3 cut(s)	249, 516, 619
BssMI	GATC	3 cut(s)	634, 868, 1027
BssNAI	GTATAC	1 cut(s)	376
BssNI	GRCGYC	2 cut(s)	207, 982
BssSI	CACGAG	1 cut(s)	444
Bst1107I	GTATAC	1 cut(s)	376
Bst2BI	CACGAG	1 cut(s)	444
Bst2UI	CCWGG	1 cut(s)	621
Bst4CI	ACNGT	1 cut(s)	1078
Bst6I	CTCTTC	1 cut(s)	9
BstACI	GRCGYC	2 cut(s)	207, 982
BstAUI	TGTACA	1 cut(s)	992
BstBI	TTCGAA	1 cut(s)	830
BstC8I	GCNNGC	2 cut(s)	117, 723
BstDEI	CTNAG	1 cut(s)	1178
BstDSI	CCRYGG	1 cut(s)	516
BstEII	GGTNACC	1 cut(s)	418
BstF5I	GGATG	8 cut(s)	136, 229, 451, 475, 526, 1012, 1096, 1288
BstHHI	GCGC	1 cut(s)	1199
BstKTI	GATC	3 cut(s)	637, 871, 1030
BstMBI	GATC	3 cut(s)	634, 868, 1027
BstMWI	GCNNNNNNNGC	5 cut(s)	25, 31, 34, 37, 512
BstNI	CCWGG	1 cut(s)	621
BstNSI	RCATGY	2 cut(s)	403, 821
BstPI	GGTNACC	1 cut(s)	418
BstSCI	CCNGG	2 cut(s)	414, 619
BstSFI	CTRYAG	2 cut(s)	315, 726
BstX2I	RGATCY	1 cut(s)	868
BstYI	RGATCY	1 cut(s)	868
BstZ17I	GTATAC	1 cut(s)	376
BsuI	GTATCC	2 cut(s)	575, 1066
BsuRI	GGCC	4 cut(s)	115, 149, 669, 861
BtgI	CCRYGG	1 cut(s)	516
BtgZI	GCGATG	1 cut(s)	599
BtsCI	GGATG	8 cut(s)	136, 229, 451, 475, 526, 1012, 1096, 1288
Cac8I	GCNNGC	2 cut(s)	117, 723
CciI	TCATGA	1 cut(s)	799
CfoI	GCGC	1 cut(s)	1199
Cfr10I	RCCGGY	1 cut(s)	115
Cfr13I	GGNCC	2 cut(s)	147, 1148
CseI	GACGC	3 cut(s)	74, 196, 990
Csp6I	GTAC	3 cut(s)	391, 397, 993
CviAII	CATG	4 cut(s)	400, 794, 800, 818
CviQI	GTAC	3 cut(s)	391, 397, 993
DdeI	CTNAG	1 cut(s)	1178
DpnI	GATC	3 cut(s)	636, 870, 1029
DpnII	GATC	3 cut(s)	634, 868, 1027
EaeI	YGGCCR	1 cut(s)	113
Eam1104I	CTCTTC	1 cut(s)	9
EarI	CTCTTC	1 cut(s)	9
EciI	GGCGGA	1 cut(s)	149
Eco24I	GRGCYC	1 cut(s)	21
Eco47I	GGWCC	1 cut(s)	1148
Eco57I	CTGAAG	2 cut(s)	393, 1110
Eco91I	GGTNACC	1 cut(s)	418
EcoO109I	RGGNCCY	1 cut(s)	147
EcoO65I	GGTNACC	1 cut(s)	418
EcoRII	CCWGG	1 cut(s)	619
EcoT38I	GRGCYC	1 cut(s)	21
FaeI	CATG	4 cut(s)	403, 797, 803, 821
FatI	CATG	4 cut(s)	399, 793, 799, 817
FbaI	TGATCA	1 cut(s)	634
FblI	GTMKAC	1 cut(s)	375
FokI	GGATG	8 cut(s)	123, 236, 438, 482, 533, 1019, 1083, 1275
FriOI	GRGCYC	1 cut(s)	21
FspBI	CTAG	2 cut(s)	722, 1294
GlaI	GCGC	1 cut(s)	1198
GsaI	CCCAGC	3 cut(s)	25, 94, 889
GsuI	CTGGAG	1 cut(s)	876
HaeIII	GGCC	4 cut(s)	115, 149, 669, 861
HapII	CCGG	4 cut(s)	116, 221, 416, 579
HgaI	GACGC	3 cut(s)	74, 196, 990
HhaI	GCGC	1 cut(s)	1199
Hin1I	GRCGYC	2 cut(s)	207, 982
Hin1II	CATG	4 cut(s)	403, 797, 803, 821
Hin6I	GCGC	1 cut(s)	1197
HinP1I	GCGC	1 cut(s)	1197
HincII	GTYRAC	1 cut(s)	1081
HindII	GTYRAC	1 cut(s)	1081
HinfI	GANTC	4 cut(s)	121, 332, 368, 1086
HpaI	GTTAAC	1 cut(s)	1081
HpaII	CCGG	4 cut(s)	116, 221, 416, 579
HphI	GGTGA	1 cut(s)	1196
Hpy166II	GTNNAC	2 cut(s)	376, 1081
Hpy188I	TCNGA	5 cut(s)	173, 250, 412, 937, 1179
Hpy188III	TCNNGA	5 cut(s)	446, 480, 800, 1004, 1151
Hpy8I	GTNNAC	2 cut(s)	376, 1081
HpyAV	CCTTC	4 cut(s)	66, 160, 659, 797
HpyCH4III	ACNGT	1 cut(s)	1078
HpyCH4IV	ACGT	1 cut(s)	490
HpyF10VI	GCNNNNNNNGC	5 cut(s)	25, 31, 34, 37, 512
HpyF3I	CTNAG	1 cut(s)	1178
HpySE526I	ACGT	1 cut(s)	490
Hsp92I	GRCGYC	2 cut(s)	207, 982
Hsp92II	CATG	4 cut(s)	403, 797, 803, 821
HspAI	GCGC	1 cut(s)	1197
KpnI	GGTACC	1 cut(s)	394
KroI	GCCGGC	1 cut(s)	115
KroNI	GCCGGC	1 cut(s)	117
Ksp22I	TGATCA	1 cut(s)	634
KspAI	GTTAAC	1 cut(s)	1081
Kzo9I	GATC	3 cut(s)	634, 868, 1027
LguI	GCTCTTC	1 cut(s)	9
LweI	GCATC	5 cut(s)	460, 502, 1141, 1183, 1231
MaeI	CTAG	2 cut(s)	722, 1294
MaeII	ACGT	1 cut(s)	490
MaeIII	GTNAC	4 cut(s)	418, 446, 1048, 1184
MalI	GATC	3 cut(s)	636, 870, 1029
MboI	GATC	3 cut(s)	634, 868, 1027
MboII	GAAGA	9 cut(s)	20, 23, 26, 341, 468, 776, 950, 1116, 1149
MfeI	CAATTG	1 cut(s)	543
MflI	RGATCY	1 cut(s)	868
MhlI	GDGCHC	1 cut(s)	21
MluCI	AATT	6 cut(s)	49, 72, 456, 543, 841, 1169
MlyI	GAGTC	1 cut(s)	377
MmeI	TCCRAC	1 cut(s)	728
MnlI	CCTC	6 cut(s)	138, 244, 355, 851, 1159, 1289
MroNI	GCCGGC	1 cut(s)	115
MseI	TTAA	2 cut(s)	552, 1080
MslI	CAYNNNNRTG	4 cut(s)	798, 804, 1023, 1179
MspA1I	CMGCKG	1 cut(s)	533
MspI	CCGG	4 cut(s)	116, 221, 416, 579
MspR9I	CCNGG	2 cut(s)	416, 621
MunI	CAATTG	1 cut(s)	543
Mva1269I	GAATGC	2 cut(s)	304, 723
MvaI	CCWGG	1 cut(s)	621
MwoI	GCNNNNNNNGC	5 cut(s)	25, 31, 34, 37, 512
NaeI	GCCGGC	1 cut(s)	117
NciI	CCSGG	1 cut(s)	416
NdeII	GATC	3 cut(s)	634, 868, 1027
NgoMIV	GCCGGC	1 cut(s)	115
NheI	GCTAGC	1 cut(s)	721
NlaIII	CATG	4 cut(s)	403, 797, 803, 821
NlaIV	GGNNCC	3 cut(s)	392, 777, 870
NmuCI	GTSAC	2 cut(s)	446, 1184
NspI	RCATGY	2 cut(s)	403, 821
NspV	TTCGAA	1 cut(s)	830
PagI	TCATGA	1 cut(s)	799
PciI	ACATGT	2 cut(s)	399, 817
PciSI	GCTCTTC	1 cut(s)	9
PctI	GAATGC	2 cut(s)	304, 723
PdiI	GCCGGC	1 cut(s)	117
PfeI	GAWTC	3 cut(s)	121, 332, 1086
PflMI	CCANNNNNTGG	1 cut(s)	287
PleI	GAGTC	1 cut(s)	376
PpsI	GAGTC	1 cut(s)	376
PscI	ACATGT	2 cut(s)	399, 817
PsiI	TTATAA	1 cut(s)	1266
Psp6I	CCWGG	1 cut(s)	619
PspEI	GGTNACC	1 cut(s)	418
PspFI	CCCAGC	3 cut(s)	21, 90, 885
PspGI	CCWGG	1 cut(s)	619
PspN4I	GGNNCC	3 cut(s)	392, 777, 870
PspPI	GGNCC	2 cut(s)	147, 1148
PsuI	RGATCY	1 cut(s)	868
PvuII	CAGCTG	1 cut(s)	533
RsaI	GTAC	3 cut(s)	392, 398, 994
RsaNI	GTAC	3 cut(s)	391, 397, 993
RseI	CAYNNNNRTG	4 cut(s)	798, 804, 1023, 1179
SapI	GCTCTTC	1 cut(s)	9
SaqAI	TTAA	2 cut(s)	552, 1080
Sau3AI	GATC	3 cut(s)	634, 868, 1027
Sau96I	GGNCC	2 cut(s)	147, 1148
SchI	GAGTC	1 cut(s)	377
ScrFI	CCNGG	2 cut(s)	416, 621
SduI	GDGCHC	1 cut(s)	21
SfaNI	GCATC	5 cut(s)	460, 502, 1141, 1183, 1231
SfcI	CTRYAG	2 cut(s)	315, 726
SfuI	TTCGAA	1 cut(s)	830
SinI	GGWCC	1 cut(s)	1148
SmiMI	CAYNNNNRTG	4 cut(s)	798, 804, 1023, 1179
SmlI	CTYRAG	1 cut(s)	200
SmoI	CTYRAG	1 cut(s)	200
Sse9I	AATT	6 cut(s)	49, 72, 456, 543, 841, 1169
SsiI	CCGC	6 cut(s)	140, 160, 163, 902, 999, 1064
SspI	AATATT	1 cut(s)	641
SspMI	CTAG	2 cut(s)	722, 1294
StyD4I	CCNGG	2 cut(s)	414, 619
TaaI	ACNGT	1 cut(s)	1078
TaiI	ACGT	1 cut(s)	493
TaqI	TCGA	4 cut(s)	129, 830, 1135, 1281
TasI	AATT	6 cut(s)	49, 72, 456, 543, 841, 1169
TatI	WGTACW	1 cut(s)	992
TauI	GCSGC	2 cut(s)	165, 1067
TfiI	GAWTC	3 cut(s)	121, 332, 1086
Tru1I	TTAA	2 cut(s)	552, 1080
Tru9I	TTAA	2 cut(s)	552, 1080
TseFI	GTSAC	2 cut(s)	446, 1184
Tsp45I	GTSAC	2 cut(s)	446, 1184
TspDTI	ATGAA	5 cut(s)	342, 449, 537, 816, 1085
TspGWI	ACGGA	2 cut(s)	967, 1098
Van91I	CCANNNNNTGG	1 cut(s)	287
VpaK11BI	GGWCC	1 cut(s)	1148
XceI	RCATGY	2 cut(s)	403, 821
XcmI	CCANNNNNNNNNTGG	1 cut(s)	462
XmiI	GTMKAC	1 cut(s)	375
XspI	CTAG	2 cut(s)	722, 1294

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.