pycom10g27030

15.4 kDa class V heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0014398
Physical Location & Seq
Reverse (-)
28137927 .. 28138681
755 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 411 bp
ATGGAGATCCCTGCCTTCCACCCTTACCAATCTGTCTTCCCTTCTCATCTCATCTATCCATACCATTTGGTCCCAGAAAACCATGTGCACTGGACTGAGACCCCTGAATCTCACATTTTCTCGGCTGACCTCCCTGGTGTGAGGAAAGAGGACATAAAAGTAGAAGTGGAGGACTCCGTGTACCTCATAATTCGAACAGAGAGGATCGAGGAGGAGTCCACAGAGCAGCCTCGCAGCAGGACCTTCATGAGGAAGTTCCGGCTTCCGGGTCGGGTCGACATCGAACGGATTTCAGCCGGATATGAAGACGGAGTGTTGACAGTTACGGTGCCGAGATATCTAAGGAGGGTTTTCTTCATTGAGCCAGCTGATGTGCCAGAAAGGCTTGAAGTTCTTGCAAGGGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

137

Amino Acids

15.96

Weight (kDa)

5.51

Isoelectric Point (pI)

68.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 31 - 112 2.4e-18 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21870
fragaria_vesca FvH4_3g02330
malus_domestica MD05G1343700.v1.1 MD10G1319400.v1.1
prunus_persica Prupe.4G023100_v2.0.a1
pyrus_communis pycom05g31310 pycom10g27030
rosa_chinensis RchiOBHm_Chr5g0003551
rosa_laevigata RLG00000031151
rosa_multiflora Rmu_sc0004279.1_g000008 Rmu_sc0009787.1_g000003
rosa_roxburghii Rroxscaffold_1G00072410
rosa_rugosa Rorug04G0404100
rosa_samantha Rh5AG031200 Rh5CG033700 Rh5DG030900
rosa_wichuraiana Rw5G002910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 328
AccI GTMKAC 1 cut(s) 276
AclWI GGATC 1 cut(s) 212
AfaI GTAC 1 cut(s) 182
AfiI CCNNNNNNNGG 2 cut(s) 249, 265
AgsI TTSAA 1 cut(s) 389
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 1 cut(s) 368
AluI AGCT 1 cut(s) 368
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 1 cut(s) 92
Alw44I GTGCAC 1 cut(s) 86
AlwI GGATC 1 cut(s) 212
ApaLI GTGCAC 1 cut(s) 86
ApeKI GCWGC 3 cut(s) 226, 234, 404
AspS9I GGNCC 2 cut(s) 70, 240
AsuC2I CCSGG 1 cut(s) 267
AsuII TTCGAA 1 cut(s) 193
AvaII GGWCC 2 cut(s) 70, 240
BaeGI GKGCMC 1 cut(s) 90
BanI GGYRCC 1 cut(s) 328
BbsI GAAGAC 2 cut(s) 28, 312
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 3 cut(s) 238, 246, 391
BciT130I CCWGG 1 cut(s) 135
BcnI CCSGG 1 cut(s) 267
BcoDI GTCTC 1 cut(s) 92
BglI GCCNNNNNGGC 1 cut(s) 382
BisI GCNGC 3 cut(s) 227, 235, 405
BlsI GCNGC 3 cut(s) 228, 236, 406
Bme1390I CCNGG 2 cut(s) 135, 267
Bme18I GGWCC 2 cut(s) 70, 240
BmgT120I GGNCC 2 cut(s) 70, 240
BmiI GGNNCC 2 cut(s) 72, 330
BmrFI CCNGG 2 cut(s) 135, 267
BpiI GAAGAC 2 cut(s) 28, 312
Bpu14I TTCGAA 1 cut(s) 193
BpuMI CCSGG 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 133
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 265
Bse1I ACTGG 1 cut(s) 95
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 1 cut(s) 133
BseLI CCNNNNNNNGG 2 cut(s) 249, 265
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 1 cut(s) 95
BseRI GAGGAG 2 cut(s) 224, 227
BseSI GKGCMC 1 cut(s) 90
BseXI GCAGC 3 cut(s) 238, 246, 391
BshNI GGYRCC 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 3 cut(s) 259, 266, 297
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 2 cut(s) 249, 265
BsmAI GTCTC 1 cut(s) 92
BsmFI GGGAC 1 cut(s) 56
Bso31I GGTCTC 1 cut(s) 92
Bsp119I TTCGAA 1 cut(s) 193
Bsp1286I GDGCHC 1 cut(s) 90
Bsp143I GATC 2 cut(s) 6, 204
BspCNI CTCAG 1 cut(s) 88
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 2 cut(s) 72, 330
BspPI GGATC 1 cut(s) 212
BspT104I TTCGAA 1 cut(s) 193
BspT107I GGYRCC 1 cut(s) 328
BspTNI GGTCTC 1 cut(s) 92
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 1 cut(s) 133
BssMI GATC 2 cut(s) 6, 204
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 2 cut(s) 322, 328
BstAPI GCANNNNNTGC 1 cut(s) 404
BstBI TTCGAA 1 cut(s) 193
BstC8I GCNNGC 1 cut(s) 366
BstDEI CTNAG 3 cut(s) 96, 341, 408
BstENI CCTNNNNNAGG 1 cut(s) 247
BstKTI GATC 2 cut(s) 9, 207
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 2 cut(s) 6, 204
BstMWI GCNNNNNNNGC 2 cut(s) 382, 404
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 2 cut(s) 133, 265
BstSLI GKGCMC 1 cut(s) 90
BstV1I GCAGC 3 cut(s) 238, 246, 391
BstV2I GAAGAC 2 cut(s) 28, 312
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
BtsIMutI CAGTG 1 cut(s) 88
Cac8I GCNNGC 1 cut(s) 366
CciI TCATGA 1 cut(s) 246
Cfr13I GGNCC 2 cut(s) 70, 240
Csp6I GTAC 1 cut(s) 181
CviAII CATG 2 cut(s) 83, 247
CviJI RGCY 8 cut(s) 125, 229, 262, 296, 364, 368, 385, 404
CviKI_1 RGCY 8 cut(s) 125, 229, 262, 296, 364, 368, 385, 404
CviQI GTAC 1 cut(s) 181
DdeI CTNAG 3 cut(s) 96, 341, 408
DpnI GATC 2 cut(s) 8, 206
DpnII GATC 2 cut(s) 6, 204
Eco31I GGTCTC 1 cut(s) 92
Eco32I GATATC 1 cut(s) 338
Eco47I GGWCC 2 cut(s) 70, 240
EcoNI CCTNNNNNAGG 1 cut(s) 247
EcoO109I RGGNCCY 1 cut(s) 240
EcoRII CCWGG 1 cut(s) 133
EcoRV GATATC 1 cut(s) 338
FaeI CATG 2 cut(s) 86, 250
FaiI YATR 6 cut(s) 61, 84, 155, 188, 248, 303
FalI AAGNNNNNCTT 1 cut(s) 391
FaqI GGGAC 1 cut(s) 56
FatI CATG 2 cut(s) 82, 246
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 3 cut(s) 227, 235, 405
Fsp4HI GCNGC 3 cut(s) 227, 235, 405
GluI GCNGC 3 cut(s) 227, 235, 405
HapII CCGG 3 cut(s) 259, 266, 297
Hin1II CATG 2 cut(s) 86, 250
HincII GTYRAC 2 cut(s) 277, 318
HindII GTYRAC 2 cut(s) 277, 318
HinfI GANTC 3 cut(s) 107, 173, 215
HpaII CCGG 3 cut(s) 259, 266, 297
Hpy166II GTNNAC 5 cut(s) 88, 181, 219, 277, 318
Hpy188III TCNNGA 1 cut(s) 247
Hpy8I GTNNAC 5 cut(s) 88, 181, 219, 277, 318
HpyAV CCTTC 3 cut(s) 25, 51, 253
HpyCH4III ACNGT 2 cut(s) 322, 328
HpyCH4V TGCA 2 cut(s) 88, 398
HpyF10VI GCNNNNNNNGC 2 cut(s) 382, 404
HpyF3I CTNAG 3 cut(s) 96, 341, 408
Hsp92II CATG 2 cut(s) 86, 250
Kzo9I GATC 2 cut(s) 6, 204
Lsp1109I GCAGC 3 cut(s) 238, 246, 391
MaeIII GTNAC 1 cut(s) 322
MalI GATC 2 cut(s) 8, 206
MboI GATC 2 cut(s) 6, 204
MboII GAAGA 3 cut(s) 28, 317, 346
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 1 cut(s) 90
MluCI AATT 1 cut(s) 189
MlyI GAGTC 2 cut(s) 167, 224
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 3 cut(s) 259, 266, 297
MspR9I CCNGG 2 cut(s) 135, 267
MvaI CCWGG 1 cut(s) 135
MwoI GCNNNNNNNGC 2 cut(s) 382, 404
NciI CCSGG 1 cut(s) 267
NdeII GATC 2 cut(s) 6, 204
NlaIII CATG 2 cut(s) 86, 250
NlaIV GGNNCC 2 cut(s) 72, 330
NmeAIII GCCGAG 2 cut(s) 101, 357
NspV TTCGAA 1 cut(s) 193
PagI TCATGA 1 cut(s) 246
PfeI GAWTC 1 cut(s) 107
PkrI GCNGC 3 cut(s) 228, 236, 406
PleI GAGTC 2 cut(s) 167, 223
PpsI GAGTC 2 cut(s) 167, 223
PpuMI RGGWCCY 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 2 cut(s) 72, 330
PspPI GGNCC 2 cut(s) 70, 240
PspPPI RGGWCCY 1 cut(s) 240
PsuI RGATCY 1 cut(s) 6
PvuII CAGCTG 1 cut(s) 368
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
SalI GTCGAC 1 cut(s) 275
SatI GCNGC 3 cut(s) 227, 235, 405
Sau3AI GATC 2 cut(s) 6, 204
Sau96I GGNCC 2 cut(s) 70, 240
SchI GAGTC 2 cut(s) 167, 224
ScrFI CCNGG 2 cut(s) 135, 267
SduI GDGCHC 1 cut(s) 90
SetI ASST 4 cut(s) 132, 186, 245, 370
SfuI TTCGAA 1 cut(s) 193
SinI GGWCC 2 cut(s) 70, 240
Sse9I AATT 1 cut(s) 189
StyD4I CCNGG 2 cut(s) 133, 265
TaaI ACNGT 2 cut(s) 322, 328
TaqI TCGA 4 cut(s) 193, 207, 276, 282
TasI AATT 1 cut(s) 189
TfiI GAWTC 1 cut(s) 107
TscAI CASTG 1 cut(s) 95
TseI GCWGC 3 cut(s) 226, 234, 404
TspDTI ATGAA 3 cut(s) 235, 318, 346
TspGWI ACGGA 3 cut(s) 166, 301, 324
TspRI CASTG 1 cut(s) 95
VneI GTGCAC 1 cut(s) 86
VpaK11BI GGWCC 2 cut(s) 70, 240
XagI CCTNNNNNAGG 1 cut(s) 247
XmiI GTMKAC 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.