pycom10g27190

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0015992
Physical Location & Seq
Reverse (-)
28234633 .. 28236382
1750 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g27190.3

Sequence Viewer

Length: 900 bp
ATGGACTTCTATAGGGACACATGTCCTCAGGCTGAAGAGGTCATCAGAGAACAAGTCAAGCTTCTCTACAAGCGCCACAAGAACACCGCATTCTCTTGGCTCAGGAACATCTTTCATGACTGTGCTGTCCAGTCATGTGATGCATCTCTGCTGCTGGACTCAACAAGGAGATCATTGTCTGAGAAGGAGATGGACAGAAGCTTTGGGATGAGAAACTTTAGGTACATTGAAGAGATTAAAGAAGCTTTGGAGAGAGAGTGCCCTGGTGTTGTTTCCTGCTCAGATATTCTCGTCTTGTCTGCCAGAGAGGGTGTTGTTAGGCTTGGAGGCCCATTCATTCCTCTTAAAACTGGAAGAAGAGATGGAAGGAGAAGCAGAGCTGAAATCCTTGAGCAATACCTTCCTGACCACAATGAGAGCATGTCCGTTGTCCTTGAGAAGTTTGCTGACATGGGCATTGACACCCCCGGTTTGGTTGCCCTTCTAGGAGCTCACAGTGTTGGAAGAACACATTGTGTGAAGCTGGTGCACCGGTTGTACCCAGAAGTTGACCCTCAGCTCAACCCTGACCATGTCCCTCACATGCTCAAGAAGTGCCCTGATGCAATCCCCGACCCCAAAGCCGTTCAATACGTGAGAAATGACCGTGGTACCCCCATGATCTTCGATAATAACTACTACAGAAACATCTTGGACAACAAGGGTTTGATGATGGTGGATCACCAGCTAGCCACAGACAAGAGGACAAAACCCTATGTCAAGAAAATGGCCAAGAGCCAAGACTACTTCTTCAAGGAGTTTTCGAGAGCCTTCACCATTCTCTCCGAGAACAACCCTCTCACCGGAAACAAGGGCGAGATCCGACAGCAGTGCAATGTTGCCAATAAAATCCGGGACTAG

Protein Analysis

300

Amino Acids

34.44

Weight (kDa)

8.69

Isoelectric Point (pI)

50.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015992)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21960
fragaria_vesca FvH4_3g02090
malus_domestica MD05G1345800.v1.1 MD10G1321200.v1.1
prunus_persica Prupe.4G021100_v2.0.a1
pyrus_communis pycom10g27190
rosa_chinensis RchiOBHm_Chr5g0003181
rosa_laevigata RLG00000031083
rosa_multiflora Rmu_sc0000146.1_g000034
rosa_roxburghii Rroxscaffold_1G00072830
rosa_rugosa Rorug04G0401100
rosa_samantha Rh5AG026000 Rh5BG027200 Rh5CG028800
rosa_wichuraiana Rw5G002410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
Acc65I GGTACC 1 cut(s) 650
AccB1I GGYRCC 1 cut(s) 650
AciI CCGC 1 cut(s) 87
AclWI GGATC 2 cut(s) 726, 853
AcoI YGGCCR 1 cut(s) 768
AcuI CTGAAG 1 cut(s) 54
AdeI CACNNNGTG 1 cut(s) 515
AfaI GTAC 3 cut(s) 224, 539, 652
AfiI CCNNNNNNNGG 2 cut(s) 472, 842
AflIII ACRYGT 1 cut(s) 20
AgeI ACCGGT 1 cut(s) 531
AgsI TTSAA 3 cut(s) 230, 629, 793
AjnI CCWGG 1 cut(s) 262
AluBI AGCT 8 cut(s) 61, 201, 245, 380, 491, 523, 559, 727
AluI AGCT 8 cut(s) 61, 201, 245, 380, 491, 523, 559, 727
Alw21I GWGCWC 2 cut(s) 493, 531
Alw44I GTGCAC 1 cut(s) 527
AlwI GGATC 2 cut(s) 726, 853
AoxI GGCC 2 cut(s) 328, 768
ApaLI GTGCAC 1 cut(s) 527
ApeKI GCWGC 1 cut(s) 151
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AsiGI ACCGGT 1 cut(s) 531
Asp718I GGTACC 1 cut(s) 650
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 1 cut(s) 329
AsuC2I CCSGG 2 cut(s) 468, 893
AsuHPI GGTGA 3 cut(s) 713, 805, 832
AsuNHI GCTAGC 1 cut(s) 727
AxyI CCTNAGG 1 cut(s) 27
BaeGI GKGCMC 3 cut(s) 263, 531, 599
BaeI ACNNNNGTAYC 2 cut(s) 634, 667
BalI TGGCCA 1 cut(s) 770
BanI GGYRCC 1 cut(s) 650
BanII GRGCYC 1 cut(s) 493
Bbv12I GWGCWC 2 cut(s) 493, 531
BbvCI CCTCAGC 1 cut(s) 555
BbvI GCAGC 1 cut(s) 138
BccI CCATC 3 cut(s) 184, 356, 706
BceAI ACGGC 1 cut(s) 608
BcgI CGANNNNNNTGC 2 cut(s) 852, 886
BciT130I CCWGG 1 cut(s) 264
BcnI CCSGG 2 cut(s) 468, 893
BfaI CTAG 3 cut(s) 485, 728, 898
BfmI CTRYAG 2 cut(s) 10, 679
BfoI RGCGCY 1 cut(s) 76
BisI GCNGC 1 cut(s) 152
BlsI GCNGC 1 cut(s) 153
Bme1390I CCNGG 3 cut(s) 264, 468, 893
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 1 cut(s) 652
BmrFI CCNGG 3 cut(s) 264, 468, 893
BmsI GCATC 3 cut(s) 130, 152, 592
BmtI GCTAGC 1 cut(s) 731
BoxI GACNNNNGTC 1 cut(s) 21
Bpu10I CCTNAGC 2 cut(s) 101, 555
BpuEI CTTGAG 3 cut(s) 410, 455, 572
BpuMI CCSGG 2 cut(s) 468, 893
BsaAI YACGTR 1 cut(s) 634
BsaJI CCNNGG 3 cut(s) 262, 466, 646
BsaWI WCCGGW 2 cut(s) 531, 842
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 2 cut(s) 472, 842
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 2 cut(s) 130, 355
Bse21I CCTNAGG 1 cut(s) 27
Bse3DI GCAATG 1 cut(s) 880
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 3 cut(s) 262, 466, 646
BseGI GGATG 1 cut(s) 213
BseLI CCNNNNNNNGG 2 cut(s) 472, 842
BseMI GCAATG 1 cut(s) 880
BseMII CTCAG 5 cut(s) 41, 115, 171, 294, 569
BseNI ACTGG 2 cut(s) 130, 355
BseSI GKGCMC 3 cut(s) 263, 531, 599
BseXI GCAGC 1 cut(s) 138
BshFI GGCC 2 cut(s) 330, 770
BshNI GGYRCC 1 cut(s) 650
BshTI ACCGGT 1 cut(s) 531
BsiHKAI GWGCWC 2 cut(s) 493, 531
BsiSI CCGG 4 cut(s) 468, 532, 843, 892
BslFI GGGAC 2 cut(s) 29, 560
BslI CCNNNNNNNGG 2 cut(s) 472, 842
BsmFI GGGAC 2 cut(s) 29, 560
BsmI GAATGC 1 cut(s) 89
BsnI GGCC 2 cut(s) 330, 770
Bsp1286I GDGCHC 4 cut(s) 263, 493, 531, 599
Bsp143I GATC 4 cut(s) 170, 660, 718, 858
BspACI CCGC 1 cut(s) 87
BspANI GGCC 2 cut(s) 330, 770
BspCNI CTCAG 5 cut(s) 40, 114, 172, 293, 568
BspHI TCATGA 1 cut(s) 115
BspLI GGNNCC 1 cut(s) 652
BspOI GCTAGC 1 cut(s) 731
BspPI GGATC 2 cut(s) 726, 853
BspT107I GGYRCC 1 cut(s) 650
BsrDI GCAATG 1 cut(s) 880
BsrFI RCCGGY 1 cut(s) 531
BsrI ACTGG 2 cut(s) 130, 355
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 3 cut(s) 262, 466, 646
BssMI GATC 4 cut(s) 170, 660, 718, 858
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 3 cut(s) 122, 497, 647
Bst6I CTCTTC 3 cut(s) 30, 225, 352
BstBAI YACGTR 1 cut(s) 634
BstC8I GCNNGC 1 cut(s) 729
BstDEI CTNAG 5 cut(s) 27, 101, 180, 280, 555
BstDSI CCRYGG 1 cut(s) 646
BstF5I GGATG 1 cut(s) 213
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 4 cut(s) 173, 663, 721, 861
BstMBI GATC 4 cut(s) 170, 660, 718, 858
BstNI CCWGG 1 cut(s) 264
BstNSI RCATGY 3 cut(s) 24, 424, 586
BstPAI GACNNNNGTC 1 cut(s) 21
BstSCI CCNGG 3 cut(s) 262, 466, 891
BstSFI CTRYAG 2 cut(s) 10, 679
BstSLI GKGCMC 3 cut(s) 263, 531, 599
BstV1I GCAGC 1 cut(s) 138
BstX2I RGATCY 1 cut(s) 858
BstYI RGATCY 1 cut(s) 858
Bsu36I CCTNAGG 1 cut(s) 27
BsuRI GGCC 2 cut(s) 330, 770
BtgI CCRYGG 1 cut(s) 646
BtsCI GGATG 1 cut(s) 213
BtsI GCAGTG 1 cut(s) 875
BtsIMutI CAGTG 2 cut(s) 502, 875
Cac8I GCNNGC 1 cut(s) 729
CciI TCATGA 1 cut(s) 115
CfoI GCGC 1 cut(s) 75
Cfr10I RCCGGY 1 cut(s) 531
Cfr13I GGNCC 1 cut(s) 329
Csp6I GTAC 3 cut(s) 223, 538, 651
CspAI ACCGGT 1 cut(s) 531
CviAII CATG 8 cut(s) 21, 116, 135, 421, 451, 572, 583, 658
CviQI GTAC 3 cut(s) 223, 538, 651
DdeI CTNAG 5 cut(s) 27, 101, 180, 280, 555
DpnI GATC 4 cut(s) 172, 662, 720, 860
DpnII GATC 4 cut(s) 170, 660, 718, 858
DraIII CACNNNGTG 1 cut(s) 515
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 1 cut(s) 768
Eam1104I CTCTTC 3 cut(s) 30, 225, 352
EarI CTCTTC 3 cut(s) 30, 225, 352
Ecl136II GAGCTC 1 cut(s) 491
Eco24I GRGCYC 1 cut(s) 493
Eco53kI GAGCTC 1 cut(s) 491
Eco57I CTGAAG 1 cut(s) 54
Eco81I CCTNAGG 1 cut(s) 27
EcoICRI GAGCTC 1 cut(s) 491
EcoRII CCWGG 1 cut(s) 262
EcoT22I ATGCAT 1 cut(s) 145
EcoT38I GRGCYC 1 cut(s) 493
FaeI CATG 8 cut(s) 24, 119, 138, 424, 454, 575, 586, 661
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FaqI GGGAC 2 cut(s) 29, 560
FatI CATG 8 cut(s) 20, 115, 134, 420, 450, 571, 582, 657
Fnu4HI GCNGC 1 cut(s) 152
FokI GGATG 1 cut(s) 220
FriOI GRGCYC 1 cut(s) 493
Fsp4HI GCNGC 1 cut(s) 152
FspBI CTAG 3 cut(s) 485, 728, 898
GlaI GCGC 1 cut(s) 74
GluI GCNGC 1 cut(s) 152
HaeII RGCGCY 1 cut(s) 76
HaeIII GGCC 2 cut(s) 330, 770
HapII CCGG 4 cut(s) 468, 532, 843, 892
HhaI GCGC 1 cut(s) 75
Hin1II CATG 8 cut(s) 24, 119, 138, 424, 454, 575, 586, 661
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HindIII AAGCTT 3 cut(s) 59, 199, 243
HinfI GANTC 1 cut(s) 158
HpaII CCGG 4 cut(s) 468, 532, 843, 892
HphI GGTGA 3 cut(s) 713, 805, 832
Hpy166II GTNNAC 2 cut(s) 529, 550
Hpy188I TCNGA 5 cut(s) 47, 181, 283, 826, 863
Hpy188III TCNNGA 6 cut(s) 103, 116, 404, 589, 760, 804
Hpy8I GTNNAC 2 cut(s) 529, 550
HpyAV CCTTC 5 cut(s) 178, 360, 410, 491, 820
HpyCH4III ACNGT 3 cut(s) 122, 497, 647
HpyCH4IV ACGT 1 cut(s) 633
HpyCH4V TGCA 4 cut(s) 143, 529, 605, 873
HpyF3I CTNAG 5 cut(s) 27, 101, 180, 280, 555
HpySE526I ACGT 1 cut(s) 633
Hsp92II CATG 8 cut(s) 24, 119, 138, 424, 454, 575, 586, 661
HspAI GCGC 1 cut(s) 73
KpnI GGTACC 1 cut(s) 654
Kzo9I GATC 4 cut(s) 170, 660, 718, 858
LmnI GCTCC 1 cut(s) 488
Lsp1109I GCAGC 1 cut(s) 138
LweI GCATC 3 cut(s) 130, 152, 592
MaeI CTAG 3 cut(s) 485, 728, 898
MaeII ACGT 1 cut(s) 633
MalI GATC 4 cut(s) 172, 662, 720, 860
MboI GATC 4 cut(s) 170, 660, 718, 858
MboII GAAGA 7 cut(s) 47, 242, 366, 369, 516, 655, 781
MflI RGATCY 1 cut(s) 858
MhlI GDGCHC 4 cut(s) 263, 493, 531, 599
MlsI TGGCCA 1 cut(s) 770
MluNI TGGCCA 1 cut(s) 770
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 2 cut(s) 481, 886
MnlI CCTC 9 cut(s) 31, 36, 301, 320, 351, 564, 588, 735, 846
Mox20I TGGCCA 1 cut(s) 770
Mph1103I ATGCAT 1 cut(s) 145
MscI TGGCCA 1 cut(s) 770
MseI TTAA 2 cut(s) 237, 345
MslI CAYNNNNRTG 1 cut(s) 120
Msp20I TGGCCA 1 cut(s) 770
MspI CCGG 4 cut(s) 468, 532, 843, 892
MspR9I CCNGG 3 cut(s) 264, 468, 893
Mva1269I GAATGC 1 cut(s) 89
MvaI CCWGG 1 cut(s) 264
NciI CCSGG 2 cut(s) 468, 893
NdeII GATC 4 cut(s) 170, 660, 718, 858
NheI GCTAGC 1 cut(s) 727
NlaIII CATG 8 cut(s) 24, 119, 138, 424, 454, 575, 586, 661
NlaIV GGNNCC 1 cut(s) 652
NsiI ATGCAT 1 cut(s) 145
NspI RCATGY 3 cut(s) 24, 424, 586
PagI TCATGA 1 cut(s) 115
PciI ACATGT 1 cut(s) 20
PctI GAATGC 1 cut(s) 89
PflFI GACNNNGTC 1 cut(s) 572
PfoI TCCNGGA 1 cut(s) 891
PinAI ACCGGT 1 cut(s) 531
PkrI GCNGC 1 cut(s) 153
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
Ppu21I YACGTR 1 cut(s) 634
PscI ACATGT 1 cut(s) 20
PshAI GACNNNNGTC 1 cut(s) 21
Psp124BI GAGCTC 1 cut(s) 493
Psp6I CCWGG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 262
PspN4I GGNNCC 1 cut(s) 652
PspPI GGNCC 1 cut(s) 329
PsuI RGATCY 1 cut(s) 858
PsyI GACNNNGTC 1 cut(s) 572
RsaI GTAC 3 cut(s) 224, 539, 652
RsaNI GTAC 3 cut(s) 223, 538, 651
RseI CAYNNNNRTG 1 cut(s) 120
SacI GAGCTC 1 cut(s) 493
SaqAI TTAA 2 cut(s) 237, 345
SatI GCNGC 1 cut(s) 152
Sau3AI GATC 4 cut(s) 170, 660, 718, 858
Sau96I GGNCC 1 cut(s) 329
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 3 cut(s) 264, 468, 893
SduI GDGCHC 4 cut(s) 263, 493, 531, 599
SfaNI GCATC 3 cut(s) 130, 152, 592
SfcI CTRYAG 2 cut(s) 10, 679
SmiMI CAYNNNNRTG 1 cut(s) 120
SmlI CTYRAG 3 cut(s) 389, 434, 587
SmoI CTYRAG 3 cut(s) 389, 434, 587
SsiI CCGC 1 cut(s) 87
SspMI CTAG 3 cut(s) 485, 728, 898
SstI GAGCTC 1 cut(s) 493
StyD4I CCNGG 3 cut(s) 262, 466, 891
TaaI ACNGT 3 cut(s) 122, 497, 647
TaiI ACGT 1 cut(s) 636
TaqI TCGA 2 cut(s) 666, 803
Tru1I TTAA 2 cut(s) 237, 345
Tru9I TTAA 2 cut(s) 237, 345
TscAI CASTG 2 cut(s) 502, 875
TseI GCWGC 1 cut(s) 151
TspDTI ATGAA 2 cut(s) 104, 325
TspGWI ACGGA 1 cut(s) 415
TspRI CASTG 2 cut(s) 502, 875
Tth111I GACNNNGTC 1 cut(s) 572
VneI GTGCAC 1 cut(s) 527
XceI RCATGY 3 cut(s) 24, 424, 586
XspI CTAG 3 cut(s) 485, 728, 898
Zsp2I ATGCAT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.