Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0015053

Physical Location & Seq

Genomic Coordinates Forward (+)

28457317 .. 28459197

Gene Structure 1881 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1881 bp

ATGCCCCAGAGAAATGTTGTTCCTTGGACTTCCATTATTGGATGTTATTCGCGTGCCGGAAATGTTGGAGTTGCGTTTGAGATGTTTAGGGAGATGCGGCGCGAAGGAGTTCAGCCCAGTTCGGTTACTCTGTTAAGCTTGACCTCCGGAGTTTCGGAGCTGCCTTATTTGCAGTGTTTGCATGGTTGTGCAGTATTATATGGTTTTGAGTCTGACATTACTTTGGTGAATTCAATGTTGAATGTGTATGGTAAATGTGGAAGAGTTGAAGATGCTCGAAACTTGTTTGAGTGTATGAATGCAAGAGACATAGTTTCATGGAATTCCTTGATTTCAAGCTATTCCCAGGCTGGAAATATCAGAGAAGTATTCCGGTTTTTGTATACAATGAGGGTTGAAGGAATGGAGCCTGATAAACAGACGTATGCATCTGCTGTGTCCGTGGCTGCCATGCAGAGTAGTTTGAAATTGGGAAAGTCAGTGCACGGACAGATTTTAAGAACTGGATATGAATTGGATTCCCATGTCGAAACAGCGCTCATAGTTATGTACTTGAAATGCAGGAACATCGACACTGCATTTCATATCTTCAAAGGGACGAGGAATAAGGATGTGGTTCTGTGGACGGCCATGATGTCAGGGCTTGTGCAAAATGATTCTGCGGACAGCGCACTAATTGTTTTCTCTCAAATGTTAGAATCAAGAACAGTGCCATCTAGTGCGACCGTAGCTAGTGCTCTTGCAGCTTGTGCGCAACTGGGTTCTTTTGATATGGGAACCTCGGTTCATGGTTATGTGCTGAGGCAAGGAATGAGGCTAGATATCCCTGCCCAGAACTCCCTTGTAACAATGTATGCAAAGTGTGCGCGTTTGGAGCAGAGTCGTGCTGTTTTCGAAAGAATGGGCAAAAGAGATTTGGTTTCTTGGAATGCCATGATTGCTGGATATGCTCAAAACGGCCATTTACGCGAGGCTCTGCTTCTCTTCTCCGAGATGAGAGCAACCCTTCAGAAGCCCGATTCTTTAACTGTGGTCTCCCTTCTCCAAGCTTGTGCTTCCATTGGGGCACTCCATCAAGGAAAGTGGATCCACAACTTTACCATTAGAAGTTGCCTTAGACCATGCATTTTGATAGACACAGCTCTGGTTGACATGTACTCAAAATGCGGTGACTTAGATAGAGCTCACAGGTGCTTCGTTGAGATGTCAGACCCGGATTTGATCTCATGGAGCACAATTATTTCCGGTTATGGTTGCCATGGAAAAGCAAAGACCGCGTTGAGGATGTATTCAGAGTTTCTGCTCACTGGAATTAAACCAAACCATGTCGTCTTCCTGTCAATTCTTTCTGCTTGTAGTCACAATGGGCTTGTTGACACAGGCTTAAGCATATACCAGTCAATGATTGCAGACTTTGGCATTGCCCCGAGCCTTGAACACCGCGCTTGCATTGTTGATCTTCTCAGTCGAGCGGGACGGGTAGAAGAGGCATATGACTTCTACAAGAGGGTCTTTCAAGAACCGGCAGTCGATGTCTTGGGCATACTTCTTGACGCTTGTCGAACGAAGGGTAATGAGGAACTGGGGAACATTATTGCCGGAGAAATTCTGATGATGCGACCTGGGGATGCTGGAAACTACGTGCAATTGGCACATAGCTACGCTTCCATGAACAGATGGGATGGCGTTGGCGAGGCATGGAGCCAAATGAGGTATCTTGGCTTGAAGAAACTCCCAGGCTGGAGTTGTATTGAGTTGCACGGGACGATCACTACATTCTACACAGATCACAACACAAATCCTCAGTTTGATGATATCGCGTCGATGTTGAAAATGTTAAGCGAGGGAATGAAGAAACCGAGCACTAACAACGAGAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1068 PPR PPR_2

Protein Analysis

627

Amino Acids

69.31

Weight (kDa)

6.76

Isoelectric Point (pI)

42.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	4 - 49	1e-09	PPR repeat family
PPR	PF01535	8 - 37	4.4e-07	PPR repeat
PPR_2	PF13041	102 - 143	3.7e-09	PPR repeat family
PPR	PF01535	105 - 134	6.6e-06	PPR repeat
PPR_2	PF13041	203 - 251	8e-06	PPR repeat family
PPR_1	PF12854	303 - 333	5e-06	PPR repeat
PPR_2	PF13041	305 - 352	2.6e-11	PPR repeat family
PPR	PF01535	307 - 333	3e-08	PPR repeat
PPR_2	PF13041	405 - 453	4.3e-06	PPR repeat family
E_motif	PF20431	525 - 585	4.7e-07	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0015053)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04370
fragaria_vesca	FvH4_3g01610
malus_domestica	MD10G1325500.v1.1
prunus_persica	Prupe.4G016600_v2.0.a1
pyrus_communis	pycom10g27520
rosa_chinensis	RchiOBHm_Chr5g0002401
rosa_laevigata	RLG00000031029
rosa_multiflora	Rmu_co8285719.1_g000001 Rmu_sc0001717.1_g000003
rosa_roxburghii	Rroxscaffold_1G00073450
rosa_rugosa	Rorug04G0396000
rosa_samantha	Rh5AG018800 Rh5BG021600 Rh5CG020700 Rh5DG019800
rosa_wichuraiana	Rw5G001760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	753
AccBSI	CCGCTC	1 cut(s)	1472
AccI	GTMKAC	1 cut(s)	383
AccII	CGCG	7 cut(s)	52, 102, 868, 969, 1277, 1443, 1820
AccIII	TCCGGA	1 cut(s)	146
AciI	CCGC	6 cut(s)	97, 662, 1167, 1275, 1441, 1472
AclWI	GGATC	2 cut(s)	1081, 1094
AcoI	YGGCCR	2 cut(s)	627, 958
AcsI	RAATTY	3 cut(s)	229, 322, 1605
AcuI	CTGAAG	1 cut(s)	992
AfaI	GTAC	2 cut(s)	551, 1157
AfeI	AGCGCT	1 cut(s)	537
AfiI	CCNNNNNNNGG	1 cut(s)	1281
AflII	CTTAAG	1 cut(s)	1384
AflIII	ACRYGT	1 cut(s)	1152
AjnI	CCWGG	3 cut(s)	345, 1621, 1735
AloI	GAACNNNNNNTCC	2 cut(s)	768, 800
AluBI	AGCT	9 cut(s)	138, 160, 339, 731, 746, 1049, 1142, 1184, 1659
AluI	AGCT	9 cut(s)	138, 160, 339, 731, 746, 1049, 1142, 1184, 1659
Alw21I	GWGCWC	5 cut(s)	486, 739, 1186, 1235, 1865
Alw26I	GTCTC	2 cut(s)	300, 1039
Alw44I	GTGCAC	1 cut(s)	482
AlwI	GGATC	2 cut(s)	1081, 1094
Ama87I	CYCGRG	1 cut(s)	1426
Aor13HI	TCCGGA	1 cut(s)	146
Aor51HI	AGCGCT	1 cut(s)	537
AoxI	GGCC	2 cut(s)	627, 958
ApaLI	GTGCAC	1 cut(s)	482
ApeKI	GCWGC	3 cut(s)	160, 446, 743
ApoI	RAATTY	3 cut(s)	229, 322, 1605
Asp700I	GAANNNNTTC	1 cut(s)	108
AspLEI	GCGC	6 cut(s)	102, 538, 671, 754, 868, 1445
AsuC2I	CCSGG	1 cut(s)	1214
AsuHPI	GGTGA	2 cut(s)	238, 1181
AsuII	TTCGAA	1 cut(s)	894
AvaI	CYCGRG	1 cut(s)	1426
BaeGI	GKGCMC	2 cut(s)	486, 1069
BamHI	GGATCC	1 cut(s)	1086
BanII	GRGCYC	1 cut(s)	1186
BbsI	GAAGAC	1 cut(s)	1324
Bbv12I	GWGCWC	5 cut(s)	486, 739, 1186, 1235, 1865
BbvCI	CCTCAGC	1 cut(s)	800
BbvI	GCAGC	3 cut(s)	147, 433, 755
BccI	CCATC	4 cut(s)	721, 1080, 1671, 1676
BceAI	ACGGC	2 cut(s)	642, 973
BcgI	CGANNNNNNTGC	2 cut(s)	550, 584
BciT130I	CCWGG	3 cut(s)	347, 1623, 1737
BcnI	CCSGG	1 cut(s)	1214
BcoDI	GTCTC	2 cut(s)	300, 1039
BfaI	CTAG	3 cut(s)	717, 732, 818
BfoI	RGCGCY	1 cut(s)	539
BfrI	CTTAAG	1 cut(s)	1384
BisI	GCNGC	4 cut(s)	98, 161, 447, 744
BlsI	GCNGC	4 cut(s)	99, 162, 448, 745
Bme1390I	CCNGG	4 cut(s)	347, 1214, 1623, 1737
BmeT110I	CYCGRG	1 cut(s)	1426
BmiI	GGNNCC	4 cut(s)	408, 778, 1088, 1703
BmrFI	CCNGG	4 cut(s)	347, 1214, 1623, 1737
BmrI	ACTGGG	3 cut(s)	111, 767, 1592
BmsI	GCATC	5 cut(s)	84, 262, 437, 1605, 1618
BmuI	ACTGGG	3 cut(s)	111, 767, 1592
BoxI	GACNNNNGTC	1 cut(s)	1557
BpiI	GAAGAC	1 cut(s)	1324
BpmI	CTGGAG	1 cut(s)	1762
Bpu10I	CCTNAGC	1 cut(s)	800
Bpu14I	TTCGAA	1 cut(s)	894
BpuMI	CCSGG	1 cut(s)	1214
BsaAI	YACGTR	1 cut(s)	1642
BsaI	GGTCTC	1 cut(s)	1039
BsaJI	CCNNGG	7 cut(s)	23, 345, 441, 780, 1258, 1622, 1735
BsaWI	WCCGGW	3 cut(s)	146, 372, 1244
Bsc4I	CCNNNNNNNGG	1 cut(s)	1281
Bse118I	RCCGGY	1 cut(s)	1522
Bse1I	ACTGG	6 cut(s)	117, 508, 762, 1312, 1396, 1587
Bse3DI	GCAATG	1 cut(s)	1419
BseAI	TCCGGA	1 cut(s)	146
BseBI	CCWGG	3 cut(s)	347, 1623, 1737
BseDI	CCNNGG	7 cut(s)	23, 345, 441, 780, 1258, 1622, 1735
BseGI	GGATG	5 cut(s)	47, 616, 1290, 1633, 1687
BseLI	CCNNNNNNNGG	1 cut(s)	1281
BseMI	GCAATG	1 cut(s)	1419
BseMII	CTCAG	3 cut(s)	791, 1477, 1817
BseNI	ACTGG	6 cut(s)	117, 508, 762, 1312, 1396, 1587
BseSI	GKGCMC	2 cut(s)	486, 1069
BseXI	GCAGC	3 cut(s)	147, 433, 755
BsgI	GTGCAG	1 cut(s)	210
Bsh1236I	CGCG	7 cut(s)	52, 102, 868, 969, 1277, 1443, 1820
Bsh1285I	CGRYCG	1 cut(s)	726
BshFI	GGCC	2 cut(s)	629, 960
BsiEI	CGRYCG	1 cut(s)	726
BsiHKAI	GWGCWC	5 cut(s)	486, 739, 1186, 1235, 1865
BsiHKCI	CYCGRG	1 cut(s)	1426
BsiSI	CCGG	7 cut(s)	57, 147, 373, 1214, 1245, 1523, 1599
BslFI	GGGAC	3 cut(s)	610, 1488, 1777
BslI	CCNNNNNNNGG	1 cut(s)	1281
BsmAI	GTCTC	2 cut(s)	300, 1039
BsmFI	GGGAC	3 cut(s)	610, 1488, 1777
BsmI	GAATGC	2 cut(s)	304, 934
BsnI	GGCC	2 cut(s)	629, 960
Bso31I	GGTCTC	1 cut(s)	1039
BsoBI	CYCGRG	1 cut(s)	1426
Bsp119I	TTCGAA	1 cut(s)	894
Bsp1286I	GDGCHC	6 cut(s)	486, 739, 1069, 1186, 1235, 1865
Bsp13I	TCCGGA	1 cut(s)	146
Bsp143I	GATC	5 cut(s)	1086, 1221, 1456, 1767, 1786
Bsp19I	CCATGG	1 cut(s)	1258
BspACI	CCGC	6 cut(s)	97, 662, 1167, 1275, 1441, 1472
BspANI	GGCC	2 cut(s)	629, 960
BspCNI	CTCAG	3 cut(s)	792, 1476, 1816
BspEI	TCCGGA	1 cut(s)	146
BspFNI	CGCG	7 cut(s)	52, 102, 868, 969, 1277, 1443, 1820
BspLI	GGNNCC	4 cut(s)	408, 778, 1088, 1703
BspPI	GGATC	2 cut(s)	1081, 1094
BspT104I	TTCGAA	1 cut(s)	894
BspTI	CTTAAG	1 cut(s)	1384
BspTNI	GGTCTC	1 cut(s)	1039
BsrBI	CCGCTC	1 cut(s)	1472
BsrDI	GCAATG	1 cut(s)	1419
BsrFI	RCCGGY	1 cut(s)	1522
BsrI	ACTGG	6 cut(s)	117, 508, 762, 1312, 1396, 1587
BssAI	RCCGGY	1 cut(s)	1522
BssECI	CCNNGG	7 cut(s)	23, 345, 441, 780, 1258, 1622, 1735
BssMI	GATC	5 cut(s)	1086, 1221, 1456, 1767, 1786
BssNAI	GTATAC	1 cut(s)	384
BssT1I	CCWWGG	2 cut(s)	23, 1258
Bst1107I	GTATAC	1 cut(s)	384
Bst2UI	CCWGG	3 cut(s)	347, 1623, 1737
Bst4CI	ACNGT	3 cut(s)	709, 727, 1030
Bst6I	CTCTTC	3 cut(s)	256, 989, 1479
BstAFI	CTTAAG	1 cut(s)	1384
BstAPI	GCANNNNNTGC	3 cut(s)	178, 749, 863
BstBAI	YACGTR	1 cut(s)	1642
BstBI	TTCGAA	1 cut(s)	894
BstC8I	GCNNGC	2 cut(s)	54, 1447
BstDEI	CTNAG	5 cut(s)	800, 1115, 1174, 1463, 1803
BstDSI	CCRYGG	2 cut(s)	441, 1258
BstF5I	GGATG	5 cut(s)	47, 616, 1290, 1633, 1687
BstFNI	CGCG	7 cut(s)	52, 102, 868, 969, 1277, 1443, 1820
BstH2I	RGCGCY	1 cut(s)	539
BstHHI	GCGC	6 cut(s)	102, 538, 671, 754, 868, 1445
BstKTI	GATC	5 cut(s)	1089, 1224, 1459, 1770, 1789
BstMAI	GTCTC	2 cut(s)	300, 1039
BstMBI	GATC	5 cut(s)	1086, 1221, 1456, 1767, 1786
BstMCI	CGRYCG	1 cut(s)	726
BstNI	CCWGG	3 cut(s)	347, 1623, 1737
BstNSI	RCATGY	1 cut(s)	1156
BstPAI	GACNNNNGTC	1 cut(s)	1557
BstSCI	CCNGG	4 cut(s)	345, 1212, 1621, 1735
BstSLI	GKGCMC	2 cut(s)	486, 1069
BstUI	CGCG	7 cut(s)	52, 102, 868, 969, 1277, 1443, 1820
BstV1I	GCAGC	3 cut(s)	147, 433, 755
BstV2I	GAAGAC	1 cut(s)	1324
BstX2I	RGATCY	1 cut(s)	1086
BstYI	RGATCY	1 cut(s)	1086
BstZ17I	GTATAC	1 cut(s)	384
BsuRI	GGCC	2 cut(s)	629, 960
BtgI	CCRYGG	2 cut(s)	441, 1258
BtsCI	GGATG	5 cut(s)	47, 616, 1290, 1633, 1687
BtsI	GCAGTG	2 cut(s)	179, 573
BtsIMutI	CAGTG	5 cut(s)	179, 486, 573, 714, 1305
Cac8I	GCNNGC	2 cut(s)	54, 1447
CfoI	GCGC	6 cut(s)	102, 538, 671, 754, 868, 1445
Cfr10I	RCCGGY	1 cut(s)	1522
CseI	GACGC	2 cut(s)	1562, 1809
Csp6I	GTAC	2 cut(s)	550, 1156
CspCI	CAANNNNNGTGG	2 cut(s)	1064, 1099
CviQI	GTAC	2 cut(s)	550, 1156
DdeI	CTNAG	5 cut(s)	800, 1115, 1174, 1463, 1803
DpnI	GATC	5 cut(s)	1088, 1223, 1458, 1769, 1788
DpnII	GATC	5 cut(s)	1086, 1221, 1456, 1767, 1786
EaeI	YGGCCR	2 cut(s)	627, 958
Eam1104I	CTCTTC	3 cut(s)	256, 989, 1479
EarI	CTCTTC	3 cut(s)	256, 989, 1479
Ecl136II	GAGCTC	1 cut(s)	1184
Eco130I	CCWWGG	2 cut(s)	23, 1258
Eco24I	GRGCYC	1 cut(s)	1186
Eco31I	GGTCTC	1 cut(s)	1039
Eco32I	GATATC	2 cut(s)	823, 1816
Eco47III	AGCGCT	1 cut(s)	537
Eco53kI	GAGCTC	1 cut(s)	1184
Eco57I	CTGAAG	1 cut(s)	992
Eco88I	CYCGRG	1 cut(s)	1426
EcoICRI	GAGCTC	1 cut(s)	1184
EcoRI	GAATTC	2 cut(s)	229, 322
EcoRII	CCWGG	3 cut(s)	345, 1621, 1735
EcoRV	GATATC	2 cut(s)	823, 1816
EcoT14I	CCWWGG	2 cut(s)	23, 1258
EcoT22I	ATGCAT	2 cut(s)	430, 1127
EcoT38I	GRGCYC	1 cut(s)	1186
ErhI	CCWWGG	2 cut(s)	23, 1258
FalI	AAGNNNNNCTT	2 cut(s)	1496, 1528
FaqI	GGGAC	3 cut(s)	610, 1488, 1777
FauI	CCCGC	1 cut(s)	1465
FauNDI	CATATG	1 cut(s)	1492
FblI	GTMKAC	1 cut(s)	383
Fnu4HI	GCNGC	4 cut(s)	98, 161, 447, 744
FokI	GGATG	5 cut(s)	54, 623, 1297, 1640, 1694
FriOI	GRGCYC	1 cut(s)	1186
Fsp4HI	GCNGC	4 cut(s)	98, 161, 447, 744
FspBI	CTAG	3 cut(s)	717, 732, 818
FspI	TGCGCA	1 cut(s)	753
GlaI	GCGC	6 cut(s)	101, 537, 670, 753, 867, 1444
GluI	GCNGC	4 cut(s)	98, 161, 447, 744
GsuI	CTGGAG	1 cut(s)	1762
HaeII	RGCGCY	1 cut(s)	539
HaeIII	GGCC	2 cut(s)	629, 960
HapII	CCGG	7 cut(s)	57, 147, 373, 1214, 1245, 1523, 1599
HgaI	GACGC	2 cut(s)	1562, 1809
HhaI	GCGC	6 cut(s)	102, 538, 671, 754, 868, 1445
Hin6I	GCGC	6 cut(s)	100, 536, 669, 752, 866, 1443
HinP1I	GCGC	6 cut(s)	100, 536, 669, 752, 866, 1443
HincII	GTYRAC	2 cut(s)	1150, 1375
HindII	GTYRAC	2 cut(s)	1150, 1375
HindIII	AAGCTT	2 cut(s)	136, 1047
HinfI	GANTC	6 cut(s)	209, 518, 656, 698, 880, 1019
HpaII	CCGG	7 cut(s)	57, 147, 373, 1214, 1245, 1523, 1599
HphI	GGTGA	2 cut(s)	238, 1181
Hpy166II	GTNNAC	5 cut(s)	384, 484, 624, 1150, 1375
Hpy188I	TCNGA	8 cut(s)	157, 214, 362, 991, 1011, 1210, 1294, 1611
Hpy188III	TCNNGA	4 cut(s)	147, 702, 1517, 1550
Hpy8I	GTNNAC	5 cut(s)	384, 484, 624, 1150, 1375
Hpy99I	CGWCG	1 cut(s)	1825
HpyAV	CCTTC	5 cut(s)	98, 392, 1016, 1049, 1561
HpyCH4III	ACNGT	3 cut(s)	709, 727, 1030
HpyCH4IV	ACGT	2 cut(s)	422, 1641
HpyF3I	CTNAG	5 cut(s)	800, 1115, 1174, 1463, 1803
HpySE526I	ACGT	2 cut(s)	422, 1641
HspAI	GCGC	6 cut(s)	100, 536, 669, 752, 866, 1443
Kpn2I	TCCGGA	1 cut(s)	146
Kzo9I	GATC	5 cut(s)	1086, 1221, 1456, 1767, 1786
LmnI	GCTCC	5 cut(s)	157, 406, 874, 1230, 1701
Lsp1109I	GCAGC	3 cut(s)	147, 433, 755
LweI	GCATC	5 cut(s)	84, 262, 437, 1605, 1618
MaeI	CTAG	3 cut(s)	717, 732, 818
MaeII	ACGT	2 cut(s)	422, 1641
MaeIII	GTNAC	4 cut(s)	124, 844, 1169, 1358
MalI	GATC	5 cut(s)	1088, 1223, 1458, 1769, 1788
MbiI	CCGCTC	1 cut(s)	1472
MboI	GATC	5 cut(s)	1086, 1221, 1456, 1767, 1786
MboII	GAAGA	9 cut(s)	273, 281, 580, 976, 1324, 1451, 1496, 1738, 1864
MfeI	CAATTG	1 cut(s)	1646
MflI	RGATCY	1 cut(s)	1086
MhlI	GDGCHC	6 cut(s)	486, 739, 1069, 1186, 1235, 1865
MlyI	GAGTC	2 cut(s)	218, 889
MmeI	TCCRAC	1 cut(s)	46
Mph1103I	ATGCAT	2 cut(s)	430, 1127
MroI	TCCGGA	1 cut(s)	146
MroXI	GAANNNNTTC	1 cut(s)	108
MseI	TTAA	6 cut(s)	134, 497, 1025, 1314, 1385, 1838
MslI	CAYNNNNRTG	3 cut(s)	186, 545, 792
MspCI	CTTAAG	1 cut(s)	1384
MspI	CCGG	7 cut(s)	57, 147, 373, 1214, 1245, 1523, 1599
MspR9I	CCNGG	4 cut(s)	347, 1214, 1623, 1737
MunI	CAATTG	1 cut(s)	1646
Mva1269I	GAATGC	2 cut(s)	304, 934
MvaI	CCWGG	3 cut(s)	347, 1623, 1737
MvnI	CGCG	7 cut(s)	52, 102, 868, 969, 1277, 1443, 1820
NciI	CCSGG	1 cut(s)	1214
NcoI	CCATGG	1 cut(s)	1258
NdeI	CATATG	1 cut(s)	1492
NdeII	GATC	5 cut(s)	1086, 1221, 1456, 1767, 1786
NlaIV	GGNNCC	4 cut(s)	408, 778, 1088, 1703
NmuCI	GTSAC	2 cut(s)	1169, 1358
NsbI	TGCGCA	1 cut(s)	753
NsiI	ATGCAT	2 cut(s)	430, 1127
NspI	RCATGY	1 cut(s)	1156
NspV	TTCGAA	1 cut(s)	894
PciI	ACATGT	1 cut(s)	1152
PctI	GAATGC	2 cut(s)	304, 934
PdmI	GAANNNNTTC	1 cut(s)	108
PfeI	GAWTC	4 cut(s)	518, 656, 698, 1019
PkrI	GCNGC	4 cut(s)	99, 162, 448, 745
PleI	GAGTC	2 cut(s)	217, 888
PpsI	GAGTC	2 cut(s)	217, 888
Ppu21I	YACGTR	1 cut(s)	1642
PscI	ACATGT	1 cut(s)	1152
PshAI	GACNNNNGTC	1 cut(s)	1557
Psp124BI	GAGCTC	1 cut(s)	1186
Psp6I	CCWGG	3 cut(s)	345, 1621, 1735
PspGI	CCWGG	3 cut(s)	345, 1621, 1735
PspN4I	GGNNCC	4 cut(s)	408, 778, 1088, 1703
PsuI	RGATCY	1 cut(s)	1086
RsaI	GTAC	2 cut(s)	551, 1157
RsaNI	GTAC	2 cut(s)	550, 1156
RseI	CAYNNNNRTG	3 cut(s)	186, 545, 792
SacI	GAGCTC	1 cut(s)	1186
SaqAI	TTAA	6 cut(s)	134, 497, 1025, 1314, 1385, 1838
SatI	GCNGC	4 cut(s)	98, 161, 447, 744
Sau3AI	GATC	5 cut(s)	1086, 1221, 1456, 1767, 1786
SchI	GAGTC	2 cut(s)	218, 889
ScrFI	CCNGG	4 cut(s)	347, 1214, 1623, 1737
SduI	GDGCHC	6 cut(s)	486, 739, 1069, 1186, 1235, 1865
SfaNI	GCATC	5 cut(s)	84, 262, 437, 1605, 1618
SfuI	TTCGAA	1 cut(s)	894
SmiMI	CAYNNNNRTG	3 cut(s)	186, 545, 792
SmlI	CTYRAG	1 cut(s)	1384
SmoI	CTYRAG	1 cut(s)	1384
SsiI	CCGC	6 cut(s)	97, 662, 1167, 1275, 1441, 1472
SspMI	CTAG	3 cut(s)	717, 732, 818
SstI	GAGCTC	1 cut(s)	1186
StyD4I	CCNGG	4 cut(s)	345, 1212, 1621, 1735
StyI	CCWWGG	2 cut(s)	23, 1258
TaaI	ACNGT	3 cut(s)	709, 727, 1030
TaiI	ACGT	2 cut(s)	425, 1644
TaqI	TCGA	8 cut(s)	277, 528, 570, 894, 1468, 1530, 1561, 1823
TatI	WGTACW	2 cut(s)	549, 1155
TauI	GCSGC	1 cut(s)	100
TfiI	GAWTC	4 cut(s)	518, 656, 698, 1019
Tru1I	TTAA	6 cut(s)	134, 497, 1025, 1314, 1385, 1838
Tru9I	TTAA	6 cut(s)	134, 497, 1025, 1314, 1385, 1838
TscAI	CASTG	5 cut(s)	179, 486, 580, 714, 1312
TseFI	GTSAC	2 cut(s)	1169, 1358
TseI	GCWGC	3 cut(s)	160, 446, 743
Tsp45I	GTSAC	2 cut(s)	1169, 1358
TspDTI	ATGAA	7 cut(s)	306, 311, 525, 572, 776, 1685, 1865
TspGWI	ACGGA	2 cut(s)	430, 501
TspRI	CASTG	5 cut(s)	179, 486, 580, 714, 1312
Vha464I	CTTAAG	1 cut(s)	1384
VneI	GTGCAC	1 cut(s)	482
XapI	RAATTY	3 cut(s)	229, 322, 1605
XceI	RCATGY	1 cut(s)	1156
XmiI	GTMKAC	1 cut(s)	383
XmnI	GAANNNNTTC	1 cut(s)	108
XspI	CTAG	3 cut(s)	717, 732, 818
Zsp2I	ATGCAT	2 cut(s)	430, 1127

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.