pycom111g01520

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0006519
Physical Location & Seq
Reverse (-)
957026 .. 959103
2078 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g01520.6

Sequence Viewer

Length: 816 bp
ATGAAATGGCTTGGTCTTCTGTACCTGTTCTTATCGGTTTCTCTGCAATTTGGTGGTGTTTCTTCAAAAAATCAGGGTCATGAGGATTCTGCAGCCACAAGAACTATAAAGAAAGAGCTGGGAGATCCACCGGAGGTACATTGTTCGAGAGAAAGGAGTAAGGCGGCGTGGAAAATAATTCAGGAGTACTTGATGCCCTTTGTAGAGAAAGAGCAGTATCAGATTTCAAGAACATGTAAGCTGCACCCTGACAATGATCTGTACAGGGATCAGGAAGAGCACAAAATCCAAGTAGATTTAAACGAATGGCAATGTGGATATTGTAAAAAGAGATTTTATGACGACAAATTTCTTGATAAGCATTTTGACAACAGGCATTACAATTTGCTGAATGTGAGTCATACCAGGTGCTTGGCAGATGTTTGTGGTGCGTTGCATTGTGACCTTGAGATGGGTTCTGTACCACACAAAAAGACTAAATGCAATCCTGCTGCTGCTGCACGGAATCGACATCTGTGTGAGGGTCTAGCTGATAGTTGTTTTCCAGTCAAACAGGGTCCAGCAGCAAGCCGTCTTCACGAGTTCTTCTTGCGCCAATTCTGTGATGCCCACACTTGTACTGGACGTCAAAAGCCCTTCTCTCAAGGCCGCAGGAAGAAGAGGGCGAGTATATCCTACCTTGTTATTTCCATTCTGACTCTGGCGTTGCTCTTCCTTTTCTACTCTTACATTTACATGTACAGCAGGGGAGTGCAAAGAGGAACTCAAGAACTGAAGCGTGTCACACCAATCGGAAGGAAGAAAAAACCGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

272

Amino Acids

31.4

Weight (kDa)

9.35

Isoelectric Point (pI)

46.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 628
AciI CCGC 2 cut(s) 164, 649
AclWI GGATC 2 cut(s) 119, 276
AcsI RAATTY 1 cut(s) 347
AcuI CTGAAG 1 cut(s) 794
AcyI GRCGYC 1 cut(s) 625
AfaI GTAC 7 cut(s) 23, 138, 188, 263, 462, 619, 740
AfiI CCNNNNNNNGG 1 cut(s) 451
AflIII ACRYGT 2 cut(s) 233, 735
AgsI TTSAA 2 cut(s) 66, 228
AjnI CCWGG 1 cut(s) 404
AluBI AGCT 3 cut(s) 118, 241, 530
AluI AGCT 3 cut(s) 118, 241, 530
Alw21I GWGCWC 1 cut(s) 282
AlwI GGATC 2 cut(s) 119, 276
AoxI GGCC 1 cut(s) 646
ApeKI GCWGC 6 cut(s) 92, 241, 491, 494, 497, 563
ApoI RAATTY 1 cut(s) 347
AspLEI GCGC 1 cut(s) 594
AspS9I GGNCC 1 cut(s) 557
AvaII GGWCC 1 cut(s) 557
BauI CACGAG 1 cut(s) 578
BbsI GAAGAC 2 cut(s) 8, 566
Bbv12I GWGCWC 1 cut(s) 282
BbvI GCAGC 6 cut(s) 104, 228, 478, 481, 484, 575
BccI CCATC 1 cut(s) 445
BceAI ACGGC 1 cut(s) 555
BciT130I CCWGG 1 cut(s) 406
BfaI CTAG 2 cut(s) 527, 814
BfmI CTRYAG 1 cut(s) 90
BisI GCNGC 8 cut(s) 93, 165, 242, 492, 495, 498, 564, 649
BlsI GCNGC 8 cut(s) 94, 166, 243, 493, 496, 499, 565, 650
BmcAI AGTACT 1 cut(s) 188
Bme1390I CCNGG 1 cut(s) 406
Bme18I GGWCC 1 cut(s) 557
BmgT120I GGNCC 1 cut(s) 557
BmiI GGNNCC 1 cut(s) 558
BmrFI CCNGG 1 cut(s) 406
BmsI GCATC 2 cut(s) 183, 595
BpiI GAAGAC 2 cut(s) 8, 566
BpuEI CTTGAG 3 cut(s) 467, 627, 750
BsaHI GRCGYC 1 cut(s) 625
BsaWI WCCGGW 1 cut(s) 130
Bsc4I CCNNNNNNNGG 1 cut(s) 451
Bse1I ACTGG 2 cut(s) 545, 625
Bse3DI GCAATG 1 cut(s) 317
BseBI CCWGG 1 cut(s) 406
BseLI CCNNNNNNNGG 1 cut(s) 451
BseMI GCAATG 1 cut(s) 317
BseNI ACTGG 2 cut(s) 545, 625
BseXI GCAGC 6 cut(s) 104, 228, 478, 481, 484, 575
BseYI CCCAGC 1 cut(s) 118
BsgI GTGCAG 2 cut(s) 227, 483
BshFI GGCC 1 cut(s) 648
BsiHKAI GWGCWC 1 cut(s) 282
BsiSI CCGG 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 451
BsnI GGCC 1 cut(s) 648
Bsp1286I GDGCHC 1 cut(s) 282
Bsp1407I TGTACA 2 cut(s) 261, 738
Bsp143I GATC 3 cut(s) 124, 256, 268
BspACI CCGC 2 cut(s) 164, 649
BspANI GGCC 1 cut(s) 648
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 558
BspMAI CTGCAG 1 cut(s) 94
BspPI GGATC 2 cut(s) 119, 276
BspQI GCTCTTC 2 cut(s) 270, 716
BsrDI GCAATG 1 cut(s) 317
BsrGI TGTACA 2 cut(s) 261, 738
BsrI ACTGG 2 cut(s) 545, 625
BssMI GATC 3 cut(s) 124, 256, 268
BssNI GRCGYC 1 cut(s) 625
BssSI CACGAG 1 cut(s) 578
Bst2BI CACGAG 1 cut(s) 578
Bst2UI CCWGG 1 cut(s) 406
Bst4CI ACNGT 1 cut(s) 810
Bst6I CTCTTC 3 cut(s) 270, 653, 716
BstACI GRCGYC 1 cut(s) 625
BstAUI TGTACA 2 cut(s) 261, 738
BstC8I GCNNGC 1 cut(s) 568
BstHHI GCGC 1 cut(s) 594
BstKTI GATC 3 cut(s) 127, 259, 271
BstMBI GATC 3 cut(s) 124, 256, 268
BstMWI GCNNNNNNNGC 1 cut(s) 497
BstNI CCWGG 1 cut(s) 406
BstNSI RCATGY 2 cut(s) 237, 739
BstSCI CCNGG 1 cut(s) 404
BstSFI CTRYAG 1 cut(s) 90
BstV1I GCAGC 6 cut(s) 104, 228, 478, 481, 484, 575
BstV2I GAAGAC 2 cut(s) 8, 566
BstX2I RGATCY 1 cut(s) 124
BstXI CCANNNNNNTGG 1 cut(s) 412
BstYI RGATCY 1 cut(s) 124
BsuRI GGCC 1 cut(s) 648
Cac8I GCNNGC 1 cut(s) 568
CciI TCATGA 1 cut(s) 79
CfoI GCGC 1 cut(s) 594
Cfr13I GGNCC 1 cut(s) 557
CsiI ACCWGGT 1 cut(s) 404
Csp6I GTAC 7 cut(s) 22, 137, 187, 262, 461, 618, 739
CviAII CATG 3 cut(s) 80, 234, 736
CviJI RGCY 8 cut(s) 10, 95, 118, 241, 530, 570, 634, 648
CviKI_1 RGCY 8 cut(s) 10, 95, 118, 241, 530, 570, 634, 648
CviQI GTAC 7 cut(s) 22, 137, 187, 262, 461, 618, 739
DpnI GATC 3 cut(s) 126, 258, 270
DpnII GATC 3 cut(s) 124, 256, 268
DraI TTTAAA 1 cut(s) 300
Eam1104I CTCTTC 3 cut(s) 270, 653, 716
EarI CTCTTC 3 cut(s) 270, 653, 716
Eco47I GGWCC 1 cut(s) 557
Eco57I CTGAAG 1 cut(s) 794
EcoRII CCWGG 1 cut(s) 404
FaeI CATG 3 cut(s) 83, 237, 739
FaiI YATR 7 cut(s) 81, 107, 235, 339, 402, 671, 737
FatI CATG 3 cut(s) 79, 233, 735
Fnu4HI GCNGC 8 cut(s) 93, 165, 242, 492, 495, 498, 564, 649
Fsp4HI GCNGC 8 cut(s) 93, 165, 242, 492, 495, 498, 564, 649
FspBI CTAG 2 cut(s) 527, 814
GlaI GCGC 1 cut(s) 593
GluI GCNGC 8 cut(s) 93, 165, 242, 492, 495, 498, 564, 649
GsaI CCCAGC 1 cut(s) 122
HaeIII GGCC 1 cut(s) 648
HapII CCGG 1 cut(s) 131
HhaI GCGC 1 cut(s) 594
Hin1I GRCGYC 1 cut(s) 625
Hin1II CATG 3 cut(s) 83, 237, 739
Hin6I GCGC 1 cut(s) 592
HinP1I GCGC 1 cut(s) 592
HinfI GANTC 4 cut(s) 86, 397, 505, 697
HpaII CCGG 1 cut(s) 131
Hpy188I TCNGA 3 cut(s) 222, 696, 794
Hpy188III TCNNGA 8 cut(s) 80, 147, 182, 228, 272, 353, 578, 767
HpyAV CCTTC 2 cut(s) 646, 789
HpyCH4III ACNGT 1 cut(s) 810
HpyCH4IV ACGT 1 cut(s) 625
HpyCH4V TGCA 7 cut(s) 46, 92, 244, 436, 483, 500, 754
HpyF10VI GCNNNNNNNGC 1 cut(s) 497
HpySE526I ACGT 1 cut(s) 625
Hsp92I GRCGYC 1 cut(s) 625
Hsp92II CATG 3 cut(s) 83, 237, 739
HspAI GCGC 1 cut(s) 592
Kzo9I GATC 3 cut(s) 124, 256, 268
LguI GCTCTTC 2 cut(s) 270, 716
Lsp1109I GCAGC 6 cut(s) 104, 228, 478, 481, 484, 575
LweI GCATC 2 cut(s) 183, 595
MabI ACCWGGT 1 cut(s) 404
MaeI CTAG 2 cut(s) 527, 814
MaeII ACGT 1 cut(s) 625
MaeIII GTNAC 2 cut(s) 440, 781
MalI GATC 3 cut(s) 126, 258, 270
MboI GATC 3 cut(s) 124, 256, 268
MboII GAAGA 9 cut(s) 8, 54, 287, 566, 577, 667, 670, 703, 811
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 5 cut(s) 47, 177, 347, 382, 596
MlyI GAGTC 2 cut(s) 406, 691
MnlI CCTC 5 cut(s) 76, 127, 514, 654, 752
MseI TTAA 1 cut(s) 299
MslI CAYNNNNRTG 2 cut(s) 516, 734
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 1 cut(s) 406
MvaI CCWGG 1 cut(s) 406
MwoI GCNNNNNNNGC 1 cut(s) 497
NdeII GATC 3 cut(s) 124, 256, 268
NlaIII CATG 3 cut(s) 83, 237, 739
NlaIV GGNNCC 1 cut(s) 558
NmuCI GTSAC 2 cut(s) 440, 781
NspI RCATGY 2 cut(s) 237, 739
PagI TCATGA 1 cut(s) 79
PciI ACATGT 2 cut(s) 233, 735
PciSI GCTCTTC 2 cut(s) 270, 716
PfeI GAWTC 2 cut(s) 86, 505
PkrI GCNGC 8 cut(s) 94, 166, 243, 493, 496, 499, 565, 650
PleI GAGTC 2 cut(s) 405, 691
PpsI GAGTC 2 cut(s) 405, 691
PscI ACATGT 2 cut(s) 233, 735
Psp6I CCWGG 1 cut(s) 404
PspFI CCCAGC 1 cut(s) 118
PspGI CCWGG 1 cut(s) 404
PspN4I GGNNCC 1 cut(s) 558
PspPI GGNCC 1 cut(s) 557
PstI CTGCAG 1 cut(s) 94
PsuI RGATCY 1 cut(s) 124
RsaI GTAC 7 cut(s) 23, 138, 188, 263, 462, 619, 740
RsaNI GTAC 7 cut(s) 22, 137, 187, 262, 461, 618, 739
RseI CAYNNNNRTG 2 cut(s) 516, 734
SapI GCTCTTC 2 cut(s) 270, 716
SaqAI TTAA 1 cut(s) 299
SatI GCNGC 8 cut(s) 93, 165, 242, 492, 495, 498, 564, 649
Sau3AI GATC 3 cut(s) 124, 256, 268
Sau96I GGNCC 1 cut(s) 557
ScaI AGTACT 1 cut(s) 188
SchI GAGTC 2 cut(s) 406, 691
ScrFI CCNGG 1 cut(s) 406
SduI GDGCHC 1 cut(s) 282
SetI ASST 9 cut(s) 27, 120, 138, 243, 410, 447, 532, 628, 681
SexAI ACCWGGT 1 cut(s) 404
SfaNI GCATC 2 cut(s) 183, 595
SfcI CTRYAG 1 cut(s) 90
SinI GGWCC 1 cut(s) 557
SmiMI CAYNNNNRTG 2 cut(s) 516, 734
SmlI CTYRAG 3 cut(s) 446, 642, 765
SmoI CTYRAG 3 cut(s) 446, 642, 765
Sse9I AATT 5 cut(s) 47, 177, 347, 382, 596
SsiI CCGC 2 cut(s) 164, 649
SspMI CTAG 2 cut(s) 527, 814
StyD4I CCNGG 1 cut(s) 404
TaaI ACNGT 1 cut(s) 810
TaiI ACGT 1 cut(s) 628
TaqI TCGA 2 cut(s) 146, 508
TasI AATT 5 cut(s) 47, 177, 347, 382, 596
TatI WGTACW 4 cut(s) 186, 261, 617, 738
TauI GCSGC 2 cut(s) 167, 651
TfiI GAWTC 2 cut(s) 86, 505
Tru1I TTAA 1 cut(s) 299
Tru9I TTAA 1 cut(s) 299
TseFI GTSAC 2 cut(s) 440, 781
TseI GCWGC 6 cut(s) 92, 241, 491, 494, 497, 563
Tsp45I GTSAC 2 cut(s) 440, 781
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 517
VpaK11BI GGWCC 1 cut(s) 557
XapI RAATTY 1 cut(s) 347
XceI RCATGY 2 cut(s) 237, 739
XcmI CCANNNNNNNNNTGG 2 cut(s) 617, 697
XspI CTAG 2 cut(s) 527, 814
ZraI GACGTC 1 cut(s) 626
ZrmI AGTACT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.