Rorug02G0574500

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006519
Physical Location & Seq
Forward (+)
69979039 .. 69981112
2074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0574500.1

Sequence Viewer

Length: 582 bp
ATGCCTTCTACTTGTCCTTGTCCACCTCCTGGTTTCAGGTTTCAGCCCTCTGATGCTGAGATTATTGGGTTTTACTTGATCAACAAGATACTGGGCAGACCGAACCCTTTTGATGTCATCAGCACCGTTGATGTCTACTCCAACAGCCCTGATCAGCTTCCACTCGGTAATGCTGGGCACTTTGAAGAGAATGAATGGTATTTCTACACAACTAGGCCAAACAGCAGCCATTTTATAACGAAAGATGGCTACTTTGCCACCTCTATGGATGACCAAATCTTTCATAACAATCAAGTGGTTGGTTTCAAGCAGATTCTGAGATTTTACCTTGGTAGACCACCAACAGGGATGAAAACCAATTGGATGGTCCATGAATTTCGTGTTAACCCTAATATATTTCCAGCAGGGAAACTTGATAATGTTCTTCAGGTGAAGCTATCCAGTTTTGTAATGTGCAAAATTCTTCATACACAAGAACTTTTTATCAAGTTGTCAGAGAAAAGAGTCAGGGATGAACGCGATTCTTTGATGATTGATACGGATGAGACGCAGAGTAAAAGGCTGAATCTACAAATTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

22.35

Weight (kDa)

6.96

Isoelectric Point (pI)

48.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 9 - 128 2.8e-28 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 236
AccB7I CCANNNNNTGG 1 cut(s) 29
AccI GTMKAC 2 cut(s) 135, 334
AccII CGCG 1 cut(s) 519
AcsI RAATTY 2 cut(s) 374, 459
AcuI CTGAAG 1 cut(s) 410
AfiI CCNNNNNNNGG 2 cut(s) 29, 344
AgsI TTSAA 2 cut(s) 185, 307
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 2 cut(s) 157, 436
AluI AGCT 2 cut(s) 157, 436
Alw26I GTCTC 1 cut(s) 539
AlwNI CAGNNNCTG 1 cut(s) 316
AoxI GGCC 1 cut(s) 215
ApeKI GCWGC 1 cut(s) 225
ApoI RAATTY 2 cut(s) 374, 459
AspS9I GGNCC 1 cut(s) 367
AsuHPI GGTGA 1 cut(s) 442
AvaII GGWCC 1 cut(s) 367
BaeGI GKGCMC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 237
BccI CCATC 2 cut(s) 239, 358
BciT130I CCWGG 1 cut(s) 30
BclI TGATCA 2 cut(s) 78, 151
BcoDI GTCTC 1 cut(s) 539
BfaI CTAG 1 cut(s) 213
BisI GCNGC 1 cut(s) 226
BlsI GCNGC 1 cut(s) 227
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 367
BmrFI CCNGG 1 cut(s) 30
BmrI ACTGGG 1 cut(s) 101
BmsI GCATC 1 cut(s) 43
BmuI ACTGGG 1 cut(s) 101
BsaJI CCNNGG 1 cut(s) 328
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 344
Bse1I ACTGG 2 cut(s) 96, 441
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 1 cut(s) 328
BseGI GGATG 5 cut(s) 274, 354, 369, 517, 547
BseLI CCNNNNNNNGG 2 cut(s) 29, 344
BseMII CTCAG 2 cut(s) 48, 308
BseNI ACTGG 2 cut(s) 96, 441
BseSI GKGCMC 1 cut(s) 180
BseXI GCAGC 1 cut(s) 237
BseYI CCCAGC 1 cut(s) 173
Bsh1236I CGCG 1 cut(s) 519
BshFI GGCC 1 cut(s) 217
BslI CCNNNNNNNGG 2 cut(s) 29, 344
BsmAI GTCTC 1 cut(s) 539
BsmBI CGTCTC 1 cut(s) 539
BsnI GGCC 1 cut(s) 217
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 2 cut(s) 78, 151
BspANI GGCC 1 cut(s) 217
BspCNI CTCAG 2 cut(s) 49, 309
BspFNI CGCG 1 cut(s) 519
BsrI ACTGG 2 cut(s) 96, 441
BssECI CCNNGG 1 cut(s) 328
BssMI GATC 2 cut(s) 78, 151
BssT1I CCWWGG 1 cut(s) 328
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 127
Bst6I CTCTTC 1 cut(s) 180
BstDEI CTNAG 2 cut(s) 57, 317
BstF5I GGATG 5 cut(s) 274, 354, 369, 517, 547
BstFNI CGCG 1 cut(s) 519
BstKTI GATC 2 cut(s) 81, 154
BstMAI GTCTC 1 cut(s) 539
BstMBI GATC 2 cut(s) 78, 151
BstNI CCWGG 1 cut(s) 30
BstSCI CCNGG 1 cut(s) 28
BstSLI GKGCMC 1 cut(s) 180
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 1 cut(s) 237
BstXI CCANNNNNNTGG 2 cut(s) 265, 364
BsuRI GGCC 1 cut(s) 217
BtsCI GGATG 5 cut(s) 274, 354, 369, 517, 547
CaiI CAGNNNCTG 1 cut(s) 316
Cfr13I GGNCC 1 cut(s) 367
CseI GACGC 1 cut(s) 556
CviAII CATG 1 cut(s) 371
CviJI RGCY 8 cut(s) 46, 147, 157, 217, 228, 249, 436, 562
CviKI_1 RGCY 8 cut(s) 46, 147, 157, 217, 228, 249, 436, 562
DdeI CTNAG 2 cut(s) 57, 317
DpnI GATC 2 cut(s) 80, 153
DpnII GATC 2 cut(s) 78, 151
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 328
Eco47I GGWCC 1 cut(s) 367
Eco57I CTGAAG 1 cut(s) 410
EcoRII CCWGG 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 328
ErhI CCWWGG 1 cut(s) 328
Esp3I CGTCTC 1 cut(s) 539
FaeI CATG 1 cut(s) 374
FaiI YATR 6 cut(s) 236, 266, 285, 372, 395, 468
FatI CATG 1 cut(s) 370
FbaI TGATCA 2 cut(s) 78, 151
FblI GTMKAC 2 cut(s) 135, 334
Fnu4HI GCNGC 1 cut(s) 226
FokI GGATG 5 cut(s) 281, 361, 376, 524, 554
Fsp4HI GCNGC 1 cut(s) 226
FspBI CTAG 1 cut(s) 213
GluI GCNGC 1 cut(s) 226
GsaI CCCAGC 1 cut(s) 177
HaeIII GGCC 1 cut(s) 217
HgaI GACGC 1 cut(s) 556
Hin1II CATG 1 cut(s) 374
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HinfI GANTC 4 cut(s) 313, 504, 521, 565
HpaI GTTAAC 1 cut(s) 385
HphI GGTGA 1 cut(s) 442
Hpy166II GTNNAC 4 cut(s) 23, 136, 335, 385
Hpy188I TCNGA 3 cut(s) 52, 318, 496
Hpy8I GTNNAC 4 cut(s) 23, 136, 335, 385
HpyAV CCTTC 1 cut(s) 15
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4V TGCA 1 cut(s) 456
HpyF3I CTNAG 2 cut(s) 57, 317
Hsp92II CATG 1 cut(s) 374
Ksp22I TGATCA 2 cut(s) 78, 151
KspAI GTTAAC 1 cut(s) 385
Kzo9I GATC 2 cut(s) 78, 151
Lsp1109I GCAGC 1 cut(s) 237
LweI GCATC 1 cut(s) 43
MaeI CTAG 1 cut(s) 213
MalI GATC 2 cut(s) 80, 153
MboI GATC 2 cut(s) 78, 151
MboII GAAGA 3 cut(s) 197, 416, 455
MfeI CAATTG 1 cut(s) 358
MhlI GDGCHC 1 cut(s) 180
MluCI AATT 4 cut(s) 358, 374, 459, 573
MlyI GAGTC 1 cut(s) 513
MmeI TCCRAC 1 cut(s) 165
MnlI CCTC 3 cut(s) 36, 58, 271
MseI TTAA 1 cut(s) 384
MslI CAYNNNNRTG 1 cut(s) 263
MspR9I CCNGG 1 cut(s) 30
MunI CAATTG 1 cut(s) 358
MvaI CCWGG 1 cut(s) 30
MvnI CGCG 1 cut(s) 519
NdeII GATC 2 cut(s) 78, 151
NlaIII CATG 1 cut(s) 374
PfeI GAWTC 3 cut(s) 313, 521, 565
PflMI CCANNNNNTGG 1 cut(s) 29
PkrI GCNGC 1 cut(s) 227
PleI GAGTC 1 cut(s) 512
PpsI GAGTC 1 cut(s) 512
PsiI TTATAA 1 cut(s) 236
Psp6I CCWGG 1 cut(s) 28
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 1 cut(s) 28
PspPI GGNCC 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 316
RseI CAYNNNNRTG 1 cut(s) 263
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 1 cut(s) 226
Sau3AI GATC 2 cut(s) 78, 151
Sau96I GGNCC 1 cut(s) 367
SchI GAGTC 1 cut(s) 513
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 1 cut(s) 180
SetI ASST 7 cut(s) 28, 41, 159, 263, 330, 432, 438
SfaNI GCATC 1 cut(s) 43
SinI GGWCC 1 cut(s) 367
SmiMI CAYNNNNRTG 1 cut(s) 263
Sse9I AATT 4 cut(s) 358, 374, 459, 573
SspMI CTAG 1 cut(s) 213
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 1 cut(s) 328
TaaI ACNGT 1 cut(s) 127
TaqII GACCGA 1 cut(s) 115
TasI AATT 4 cut(s) 358, 374, 459, 573
TfiI GAWTC 3 cut(s) 313, 521, 565
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TseI GCWGC 1 cut(s) 225
TspDTI ATGAA 6 cut(s) 207, 272, 365, 387, 455, 528
TspGWI ACGGA 1 cut(s) 554
Van91I CCANNNNNTGG 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 367
XapI RAATTY 2 cut(s) 374, 459
XmiI GTMKAC 2 cut(s) 135, 334
XspI CTAG 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.