pycom111g02420

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0009157
Physical Location & Seq
Forward (+)
1441839 .. 1444137
2299 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1695 bp
ATGAGGACGTTTAACCTAAAAACCCTCTTGTTATTTGTTCTCATTGCGTTTATTATCTCGTTTTCGAGTTTTTTCAAACTATGTGATGCTCGAGAAGGAGGCAATAAGCAGCACTGGAGGAAGAAACTAAGGGGTTATGGTGGTGATCATCGCCGCGCTGCTTCAAGGGGAGAGAGTTACATCACACCGGAGGAAACTGCGGTGACACTTGGAAAAAAGAGCGGTGGGGGTTCGGACGTGTTTAATGTGTTGGACTATGGTGCTAAAGGTGATGGAAAAGCTGATGACACCAAGGCATTCGAGGCGGCGTGGGCAGCAGCTTGCAAAGTGCAGGCATCAACTATAAGTGTTCCATCCGGGTCTGTATTCCTTGTCAAACCAATCTCTTTCTCTGGCCCTAACTGCGAAGCAAATATCGTGTTTCAGTTAGATGGTAAAATCATAGCTCCAACAAGCTCAGGCGATTGGGGTTCAGGTCTTTTACAATGGATTGAATTCACAAAACTAAAAGGGAGATCGATCAAAGGCACCGGCGTCATTGATGGACAAGGCTCGGTTTGGTGGAATGATTCGCCGAAAGACAATCCTGCTAACGAAACAACGGATAGCAGTCTCGATGATTCGGTAACTAACTGTCCCTCCATATGCATCTCTCGAAATAGTCAAGTAAAGCAGTCCGGCCAAATGCTTTTATTCAGCTTAAAAAAATCATCATGTTCACTTGTGTTAATTTATAGTGAAACTAATCGAGCATGCGTGCTTTTCTCATGTCAACTTCATCAGCAGGCCACAGAAAGTTACAGTGGGAAAATGCCAAGCACCAAACCAACGGCTCTTAGGTTCTATGGAAGCGACCTCGTTAGTGTCACTGGCATAACACTTCAAAACAGCCCTCAGGCGCACCTCAAGTTCGACGCCTGCACAAATGTTCAGGTTTCCGGTATAAGTATTTCATCCCCGGGCAACAGCCCCAACACGGATGGAATCCACCTCCAAAACTCCCAAAATGCCGTCATTTCCGCCAGCAGTATCGCTTGTGAAAGAAAGTTGACACAATATCGACAACAATATAATGAACTGCAACATATAATGACTGCAGGAGATGACTGTGTATCGATACAAACTGGATGCTCGAACGTATACATACACAACGTAAATTGTGGACCTGGACATGGAGTCAGCATTGGAGGGCTAGGGAAGGACAACACGAAAGCTTGCGTTTCGAACGTCACTGTCCGAGACGTTAAAATGCACAGTACACTCACGGGTGTCAGAATAAAGACCTGGCAGGTACGTACTGGAGGATCGGGGTCTGTCCAAAACATAATGTTCTCAAACATTCAACTAACAGAAGTTGAAACGCCGATCATGATTGACCAGTTCTACTGCGACAAGAGCAAGTGCCAAAATCAGAGCTCCGCTGTGGCGATTTCGGGTGTGAATTACGTAAACATACAAGGAACCTACACGTCGAAGGCCGTGCATTTCGCATGCAGTGACAGCTCTCCGTGCACCGGTGTGTCTCTGGATACCATAGAGTTGAAATCAGTTGATGAAGGGAAGCACCTGTACGACCCTTTCTGTTGGCAGGCGTACGGAGTGCTCAAAACAAGCACCGTTCCTCGAATCGACTGCTTGAAGAAGGGGAAGCCGAGCACAATAACTCAATCCGGAAGTAGTGGTTCTTCGTGCTAA

Protein Analysis

565

Amino Acids

60.62

Weight (kDa)

8.61

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 81 - 128 9.7e-07 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 110 - 206 1.9e-11 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 272 - 345 3.8e-14 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 363 - 516 5.5e-45 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1279
AccB1I GGYRCC 1 cut(s) 527
AccBSI CCGCTC 1 cut(s) 222
AccI GTMKAC 1 cut(s) 1140
AccII CGCG 1 cut(s) 156
AccIII TCCGGA 1 cut(s) 1670
AciI CCGC 6 cut(s) 154, 200, 222, 305, 1020, 1419
AclWI GGATC 1 cut(s) 1312
AcoI YGGCCR 1 cut(s) 679
AcsI RAATTY 1 cut(s) 494
AcyI GRCGYC 2 cut(s) 534, 915
AfaI GTAC 5 cut(s) 1258, 1293, 1297, 1571, 1595
AfiI CCNNNNNNNGG 3 cut(s) 976, 1172, 1514
AflIII ACRYGT 2 cut(s) 237, 1467
AgeI ACCGGT 1 cut(s) 1514
AgsI TTSAA 8 cut(s) 76, 165, 494, 884, 1343, 1358, 1543, 1639
AhdI GACNNNNNGTC 1 cut(s) 1175
AjiI CACGTC 2 cut(s) 238, 1470
AjnI CCWGG 2 cut(s) 1165, 1283
AleI CACNNNNGTG 1 cut(s) 1517
AluBI AGCT 8 cut(s) 281, 320, 446, 456, 699, 1214, 1416, 1503
AluI AGCT 8 cut(s) 281, 320, 446, 456, 699, 1214, 1416, 1503
Alw21I GWGCWC 4 cut(s) 1418, 1514, 1605, 1658
Alw26I GTCTC 3 cut(s) 617, 1233, 1527
Alw44I GTGCAC 1 cut(s) 1510
AlwI GGATC 1 cut(s) 1312
Ama87I CYCGRG 2 cut(s) 90, 958
Aor13HI TCCGGA 1 cut(s) 1670
AoxI GGCC 4 cut(s) 394, 679, 786, 1476
ApaLI GTGCAC 1 cut(s) 1510
ApeKI GCWGC 4 cut(s) 109, 158, 314, 317
ApoI RAATTY 1 cut(s) 494
AsiGI ACCGGT 1 cut(s) 1514
AspLEI GCGC 2 cut(s) 158, 901
AspS9I GGNCC 2 cut(s) 395, 1163
AsuC2I CCSGG 3 cut(s) 358, 959, 960
AsuHPI GGTGA 3 cut(s) 155, 214, 281
AsuII TTCGAA 1 cut(s) 1223
AvaI CYCGRG 2 cut(s) 90, 958
AvaII GGWCC 1 cut(s) 1163
AxyI CCTNAGG 1 cut(s) 894
BaeGI GKGCMC 1 cut(s) 1514
BanI GGYRCC 1 cut(s) 527
BanII GRGCYC 1 cut(s) 1418
BarI GAAGNNNNNNTAC 3 cut(s) 1553, 1585, 1669
Bbv12I GWGCWC 4 cut(s) 1418, 1514, 1605, 1658
BbvI GCAGC 4 cut(s) 121, 145, 326, 329
BccI CCATC 5 cut(s) 266, 361, 425, 536, 974
BceAI ACGGC 3 cut(s) 846, 995, 1463
BcgI CGANNNNNNTGC 4 cut(s) 1462, 1496, 1614, 1648
BciT130I CCWGG 2 cut(s) 1167, 1285
BciVI GTATCC 1 cut(s) 1522
BclI TGATCA 1 cut(s) 145
BcnI CCSGG 3 cut(s) 358, 959, 960
BcoDI GTCTC 3 cut(s) 617, 1233, 1527
BfaI CTAG 1 cut(s) 1193
BfmI CTRYAG 1 cut(s) 1095
BfuAI ACCTGC 1 cut(s) 1279
BfuI GTATCC 1 cut(s) 1522
BisI GCNGC 6 cut(s) 110, 154, 159, 306, 315, 318
BlsI GCNGC 6 cut(s) 111, 155, 160, 307, 316, 319
Bme1390I CCNGG 5 cut(s) 358, 959, 960, 1167, 1285
Bme18I GGWCC 1 cut(s) 1163
BmeRI GACNNNNNGTC 1 cut(s) 1175
BmeT110I CYCGRG 2 cut(s) 90, 958
BmgBI CACGTC 2 cut(s) 238, 1470
BmgT120I GGNCC 2 cut(s) 395, 1163
BmiI GGNNCC 2 cut(s) 529, 1462
BmrFI CCNGG 5 cut(s) 358, 959, 960, 1167, 1285
BmsI GCATC 4 cut(s) 76, 344, 657, 1118
BpmI CTGGAG 2 cut(s) 136, 1320
Bpu10I CCTNAGC 1 cut(s) 457
Bpu14I TTCGAA 1 cut(s) 1223
BpuEI CTTGAG 1 cut(s) 890
BpuMI CCSGG 3 cut(s) 358, 959, 960
Bsa29I ATCGAT 2 cut(s) 518, 1115
BsaAI YACGTR 2 cut(s) 1295, 1447
BsaHI GRCGYC 2 cut(s) 534, 915
BsaJI CCNNGG 3 cut(s) 291, 957, 958
BsaWI WCCGGW 4 cut(s) 187, 938, 1514, 1670
BsaXI ACNNNNNCTCC 2 cut(s) 623, 653
Bsc4I CCNNNNNNNGG 3 cut(s) 976, 1172, 1514
Bse118I RCCGGY 2 cut(s) 530, 1514
Bse1I ACTGG 5 cut(s) 119, 874, 1129, 1303, 1378
Bse21I CCTNAGG 1 cut(s) 894
Bse3DI GCAATG 1 cut(s) 42
BseAI TCCGGA 1 cut(s) 1670
BseBI CCWGG 2 cut(s) 1167, 1285
BseCI ATCGAT 2 cut(s) 518, 1115
BseDI CCNNGG 3 cut(s) 291, 957, 958
BseGI GGATG 4 cut(s) 353, 953, 985, 1133
BseLI CCNNNNNNNGG 3 cut(s) 976, 1172, 1514
BseMI GCAATG 1 cut(s) 42
BseMII CTCAG 2 cut(s) 471, 908
BseNI ACTGG 5 cut(s) 119, 874, 1129, 1303, 1378
BseSI GKGCMC 1 cut(s) 1514
BseXI GCAGC 4 cut(s) 121, 145, 326, 329
BsgI GTGCAG 2 cut(s) 350, 904
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 4 cut(s) 396, 681, 788, 1478
BshNI GGYRCC 1 cut(s) 527
BshTI ACCGGT 1 cut(s) 1514
BshVI ATCGAT 2 cut(s) 518, 1115
BsiHKAI GWGCWC 4 cut(s) 1418, 1514, 1605, 1658
BsiHKCI CYCGRG 2 cut(s) 90, 958
BsiSI CCGG 8 cut(s) 188, 357, 531, 678, 939, 959, 1515, 1671
BsiWI CGTACG 1 cut(s) 1593
BslFI GGGAC 1 cut(s) 621
BslI CCNNNNNNNGG 3 cut(s) 976, 1172, 1514
BsmAI GTCTC 3 cut(s) 617, 1233, 1527
BsmBI CGTCTC 1 cut(s) 1233
BsmFI GGGAC 1 cut(s) 621
BsmI GAATGC 1 cut(s) 296
BsnI GGCC 4 cut(s) 396, 681, 788, 1478
BsoBI CYCGRG 2 cut(s) 90, 958
Bsp119I TTCGAA 1 cut(s) 1223
Bsp1286I GDGCHC 4 cut(s) 1418, 1514, 1605, 1658
Bsp13I TCCGGA 1 cut(s) 1670
Bsp143I GATC 5 cut(s) 145, 515, 519, 1304, 1365
BspACI CCGC 6 cut(s) 154, 200, 222, 305, 1020, 1419
BspANI GGCC 4 cut(s) 396, 681, 788, 1478
BspCNI CTCAG 2 cut(s) 470, 907
BspDI ATCGAT 2 cut(s) 518, 1115
BspEI TCCGGA 1 cut(s) 1670
BspFNI CGCG 1 cut(s) 156
BspHI TCATGA 1 cut(s) 1368
BspLI GGNNCC 2 cut(s) 529, 1462
BspMAI CTGCAG 1 cut(s) 1099
BspMI ACCTGC 1 cut(s) 1279
BspPI GGATC 1 cut(s) 1312
BspT104I TTCGAA 1 cut(s) 1223
BspT107I GGYRCC 1 cut(s) 527
BsrBI CCGCTC 1 cut(s) 222
BsrDI GCAATG 1 cut(s) 42
BsrFI RCCGGY 2 cut(s) 530, 1514
BsrI ACTGG 5 cut(s) 119, 874, 1129, 1303, 1378
BssAI RCCGGY 2 cut(s) 530, 1514
BssECI CCNNGG 3 cut(s) 291, 957, 958
BssMI GATC 5 cut(s) 145, 515, 519, 1304, 1365
BssNAI GTATAC 1 cut(s) 1141
BssNI GRCGYC 2 cut(s) 534, 915
BssT1I CCWWGG 1 cut(s) 291
Bst1107I GTATAC 1 cut(s) 1141
Bst2UI CCWGG 2 cut(s) 1167, 1285
Bst4CI ACNGT 6 cut(s) 635, 803, 1109, 1234, 1256, 1618
BstACI GRCGYC 2 cut(s) 534, 915
BstBAI YACGTR 2 cut(s) 1295, 1447
BstBI TTCGAA 1 cut(s) 1223
BstDEI CTNAG 4 cut(s) 128, 457, 836, 894
BstF5I GGATG 4 cut(s) 353, 953, 985, 1133
BstFNI CGCG 1 cut(s) 156
BstHHI GCGC 2 cut(s) 158, 901
BstKTI GATC 5 cut(s) 148, 518, 522, 1307, 1368
BstMAI GTCTC 3 cut(s) 617, 1233, 1527
BstMBI GATC 5 cut(s) 145, 515, 519, 1304, 1365
BstMWI GCNNNNNNNGC 7 cut(s) 302, 311, 314, 402, 1395, 1500, 1509
BstNI CCWGG 2 cut(s) 1167, 1285
BstNSI RCATGY 2 cut(s) 756, 1494
BstSCI CCNGG 5 cut(s) 356, 957, 958, 1165, 1283
BstSFI CTRYAG 1 cut(s) 1095
BstSLI GKGCMC 1 cut(s) 1514
BstSNI TACGTA 2 cut(s) 1295, 1447
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 4 cut(s) 121, 145, 326, 329
BstZ17I GTATAC 1 cut(s) 1141
Bsu15I ATCGAT 2 cut(s) 518, 1115
Bsu36I CCTNAGG 1 cut(s) 894
BsuI GTATCC 1 cut(s) 1522
BsuRI GGCC 4 cut(s) 396, 681, 788, 1478
BsuTUI ATCGAT 2 cut(s) 518, 1115
BtgZI GCGATG 1 cut(s) 134
BtrI CACGTC 2 cut(s) 238, 1470
BtsCI GGATG 4 cut(s) 353, 953, 985, 1133
BtsI GCAGTG 1 cut(s) 1501
BtsIMutI CAGTG 5 cut(s) 112, 808, 867, 1230, 1501
BveI ACCTGC 1 cut(s) 1279
CciI TCATGA 1 cut(s) 1368
CfoI GCGC 2 cut(s) 158, 901
Cfr10I RCCGGY 2 cut(s) 530, 1514
Cfr13I GGNCC 2 cut(s) 395, 1163
Cfr9I CCCGGG 1 cut(s) 958
ClaI ATCGAT 2 cut(s) 518, 1115
CseI GACGC 2 cut(s) 523, 923
Csp6I GTAC 5 cut(s) 1257, 1292, 1296, 1570, 1594
CspAI ACCGGT 1 cut(s) 1514
CviAII CATG 6 cut(s) 714, 753, 768, 1172, 1369, 1491
CviQI GTAC 5 cut(s) 1257, 1292, 1296, 1570, 1594
DdeI CTNAG 4 cut(s) 128, 457, 836, 894
DpnI GATC 5 cut(s) 147, 517, 521, 1306, 1367
DpnII GATC 5 cut(s) 145, 515, 519, 1304, 1365
DriI GACNNNNNGTC 1 cut(s) 1175
EaeI YGGCCR 1 cut(s) 679
Eam1105I GACNNNNNGTC 1 cut(s) 1175
EciI GGCGGA 1 cut(s) 1009
Ecl136II GAGCTC 1 cut(s) 1416
Eco105I TACGTA 2 cut(s) 1295, 1447
Eco130I CCWWGG 1 cut(s) 291
Eco24I GRGCYC 1 cut(s) 1418
Eco47I GGWCC 1 cut(s) 1163
Eco53kI GAGCTC 1 cut(s) 1416
Eco81I CCTNAGG 1 cut(s) 894
Eco88I CYCGRG 2 cut(s) 90, 958
EcoICRI GAGCTC 1 cut(s) 1416
EcoRI GAATTC 1 cut(s) 494
EcoRII CCWGG 2 cut(s) 1165, 1283
EcoT14I CCWWGG 1 cut(s) 291
EcoT22I ATGCAT 1 cut(s) 650
EcoT38I GRGCYC 1 cut(s) 1418
ErhI CCWWGG 1 cut(s) 291
Esp3I CGTCTC 1 cut(s) 1233
FaeI CATG 6 cut(s) 717, 756, 771, 1175, 1372, 1494
FaqI GGGAC 1 cut(s) 621
FatI CATG 6 cut(s) 713, 752, 767, 1171, 1368, 1490
FauNDI CATATG 1 cut(s) 644
FbaI TGATCA 1 cut(s) 145
FblI GTMKAC 1 cut(s) 1140
Fnu4HI GCNGC 6 cut(s) 110, 154, 159, 306, 315, 318
FokI GGATG 4 cut(s) 340, 940, 992, 1140
FriOI GRGCYC 1 cut(s) 1418
Fsp4HI GCNGC 6 cut(s) 110, 154, 159, 306, 315, 318
FspBI CTAG 1 cut(s) 1193
GlaI GCGC 2 cut(s) 157, 900
GluI GCNGC 6 cut(s) 110, 154, 159, 306, 315, 318
GsuI CTGGAG 2 cut(s) 136, 1320
HaeIII GGCC 4 cut(s) 396, 681, 788, 1478
HapII CCGG 8 cut(s) 188, 357, 531, 678, 939, 959, 1515, 1671
HgaI GACGC 2 cut(s) 523, 923
HhaI GCGC 2 cut(s) 158, 901
Hin1I GRCGYC 2 cut(s) 534, 915
Hin1II CATG 6 cut(s) 717, 756, 771, 1175, 1372, 1494
Hin6I GCGC 2 cut(s) 156, 899
HinP1I GCGC 2 cut(s) 156, 899
HincII GTYRAC 2 cut(s) 773, 1050
HindII GTYRAC 2 cut(s) 773, 1050
HindIII AAGCTT 1 cut(s) 1212
HinfI GANTC 5 cut(s) 569, 620, 984, 1176, 1626
HpaII CCGG 8 cut(s) 188, 357, 531, 678, 939, 959, 1515, 1671
HphI GGTGA 3 cut(s) 155, 214, 281
Hpy166II GTNNAC 8 cut(s) 719, 773, 1050, 1141, 1163, 1259, 1450, 1512
Hpy188I TCNGA 4 cut(s) 235, 1238, 1274, 1413
Hpy188III TCNNGA 6 cut(s) 92, 614, 654, 1369, 1526, 1671
Hpy8I GTNNAC 8 cut(s) 719, 773, 1050, 1141, 1163, 1259, 1450, 1512
Hpy99I CGWCG 2 cut(s) 917, 1474
HpyAV CCTTC 5 cut(s) 89, 1192, 1468, 1550, 1636
HpyCH4III ACNGT 6 cut(s) 635, 803, 1109, 1234, 1256, 1618
HpyCH4IV ACGT 9 cut(s) 8, 237, 1137, 1152, 1227, 1242, 1294, 1446, 1469
HpyF10VI GCNNNNNNNGC 7 cut(s) 302, 311, 314, 402, 1395, 1500, 1509
HpyF3I CTNAG 4 cut(s) 128, 457, 836, 894
HpySE526I ACGT 9 cut(s) 8, 237, 1137, 1152, 1227, 1242, 1294, 1446, 1469
Hsp92I GRCGYC 2 cut(s) 534, 915
Hsp92II CATG 6 cut(s) 717, 756, 771, 1175, 1372, 1494
HspAI GCGC 2 cut(s) 156, 899
Kpn2I TCCGGA 1 cut(s) 1670
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 5 cut(s) 145, 515, 519, 1304, 1365
LmnI GCTCC 2 cut(s) 451, 1421
Lsp1109I GCAGC 4 cut(s) 121, 145, 326, 329
LweI GCATC 4 cut(s) 76, 344, 657, 1118
MaeI CTAG 1 cut(s) 1193
MaeII ACGT 9 cut(s) 8, 237, 1137, 1152, 1227, 1242, 1294, 1446, 1469
MaeIII GTNAC 7 cut(s) 176, 202, 625, 797, 865, 1228, 1496
MalI GATC 5 cut(s) 147, 517, 521, 1306, 1367
MbiI CCGCTC 1 cut(s) 222
MboI GATC 5 cut(s) 145, 515, 519, 1304, 1365
MboII GAAGA 3 cut(s) 133, 1651, 1677
MhlI GDGCHC 4 cut(s) 1418, 1514, 1605, 1658
MluCI AATT 4 cut(s) 494, 729, 1156, 1441
MlyI GAGTC 1 cut(s) 1185
MmeI TCCRAC 2 cut(s) 231, 473
Mph1103I ATGCAT 1 cut(s) 650
MroI TCCGGA 1 cut(s) 1670
MseI TTAA 5 cut(s) 12, 243, 701, 728, 1245
MslI CAYNNNNRTG 1 cut(s) 1517
MspA1I CMGCKG 1 cut(s) 1421
MspI CCGG 8 cut(s) 188, 357, 531, 678, 939, 959, 1515, 1671
MspR9I CCNGG 5 cut(s) 358, 959, 960, 1167, 1285
Mva1269I GAATGC 1 cut(s) 296
MvaI CCWGG 2 cut(s) 1167, 1285
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 7 cut(s) 302, 311, 314, 402, 1395, 1500, 1509
NciI CCSGG 3 cut(s) 358, 959, 960
NdeI CATATG 1 cut(s) 644
NdeII GATC 5 cut(s) 145, 515, 519, 1304, 1365
NlaIII CATG 6 cut(s) 717, 756, 771, 1175, 1372, 1494
NlaIV GGNNCC 2 cut(s) 529, 1462
NmeAIII GCCGAG 1 cut(s) 1677
NmuCI GTSAC 4 cut(s) 202, 865, 1228, 1496
NsiI ATGCAT 1 cut(s) 650
NspI RCATGY 2 cut(s) 756, 1494
NspV TTCGAA 1 cut(s) 1223
OliI CACNNNNGTG 1 cut(s) 1517
PaeI GCATGC 2 cut(s) 756, 1494
PaeR7I CTCGAG 1 cut(s) 90
PagI TCATGA 1 cut(s) 1368
PctI GAATGC 1 cut(s) 296
PfeI GAWTC 4 cut(s) 569, 620, 984, 1626
Pfl23II CGTACG 1 cut(s) 1593
PinAI ACCGGT 1 cut(s) 1514
PkrI GCNGC 6 cut(s) 111, 155, 160, 307, 316, 319
PleI GAGTC 1 cut(s) 1184
PpsI GAGTC 1 cut(s) 1184
Ppu21I YACGTR 2 cut(s) 1295, 1447
Psp124BI GAGCTC 1 cut(s) 1418
Psp6I CCWGG 2 cut(s) 1165, 1283
PspGI CCWGG 2 cut(s) 1165, 1283
PspLI CGTACG 1 cut(s) 1593
PspN4I GGNNCC 2 cut(s) 529, 1462
PspPI GGNCC 2 cut(s) 395, 1163
PstI CTGCAG 1 cut(s) 1099
RsaI GTAC 5 cut(s) 1258, 1293, 1297, 1571, 1595
RsaNI GTAC 5 cut(s) 1257, 1292, 1296, 1570, 1594
RseI CAYNNNNRTG 1 cut(s) 1517
SacI GAGCTC 1 cut(s) 1418
SaqAI TTAA 5 cut(s) 12, 243, 701, 728, 1245
SatI GCNGC 6 cut(s) 110, 154, 159, 306, 315, 318
Sau3AI GATC 5 cut(s) 145, 515, 519, 1304, 1365
Sau96I GGNCC 2 cut(s) 395, 1163
SchI GAGTC 1 cut(s) 1185
ScrFI CCNGG 5 cut(s) 358, 959, 960, 1167, 1285
SduI GDGCHC 4 cut(s) 1418, 1514, 1605, 1658
SfaNI GCATC 4 cut(s) 76, 344, 657, 1118
SfcI CTRYAG 1 cut(s) 1095
Sfr274I CTCGAG 1 cut(s) 90
SfuI TTCGAA 1 cut(s) 1223
SgrAI CRCCGGYG 2 cut(s) 530, 1514
SinI GGWCC 1 cut(s) 1163
SlaI CTCGAG 1 cut(s) 90
SmaI CCCGGG 1 cut(s) 960
SmiMI CAYNNNNRTG 1 cut(s) 1517
SmlI CTYRAG 2 cut(s) 90, 905
SmoI CTYRAG 2 cut(s) 90, 905
SnaBI TACGTA 2 cut(s) 1295, 1447
SphI GCATGC 2 cut(s) 756, 1494
Sse9I AATT 4 cut(s) 494, 729, 1156, 1441
SsiI CCGC 6 cut(s) 154, 200, 222, 305, 1020, 1419
SspMI CTAG 1 cut(s) 1193
SstI GAGCTC 1 cut(s) 1418
StyD4I CCNGG 5 cut(s) 356, 957, 958, 1165, 1283
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 6 cut(s) 635, 803, 1109, 1234, 1256, 1618
TaiI ACGT 9 cut(s) 11, 240, 1140, 1155, 1230, 1245, 1297, 1449, 1472
TasI AATT 4 cut(s) 494, 729, 1156, 1441
TatI WGTACW 1 cut(s) 1256
TauI GCSGC 2 cut(s) 156, 308
TfiI GAWTC 4 cut(s) 569, 620, 984, 1626
Tru1I TTAA 5 cut(s) 12, 243, 701, 728, 1245
Tru9I TTAA 5 cut(s) 12, 243, 701, 728, 1245
TscAI CASTG 5 cut(s) 119, 808, 874, 1237, 1501
TseFI GTSAC 4 cut(s) 202, 865, 1228, 1496
TseI GCWGC 4 cut(s) 109, 158, 314, 317
Tsp45I GTSAC 4 cut(s) 202, 865, 1228, 1496
TspDTI ATGAA 4 cut(s) 767, 942, 1089, 1569
TspGWI ACGGA 4 cut(s) 617, 992, 1497, 1611
TspMI CCCGGG 1 cut(s) 958
TspRI CASTG 5 cut(s) 119, 808, 874, 1237, 1501
VneI GTGCAC 1 cut(s) 1510
VpaK11BI GGWCC 1 cut(s) 1163
XapI RAATTY 1 cut(s) 494
XceI RCATGY 2 cut(s) 756, 1494
XhoI CTCGAG 1 cut(s) 90
XmaI CCCGGG 1 cut(s) 958
XmiI GTMKAC 1 cut(s) 1140
XspI CTAG 1 cut(s) 1193
Zsp2I ATGCAT 1 cut(s) 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.