pycom111g02900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0017084
Physical Location & Seq
Forward (+)
1736407 .. 1736886
480 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g02900.1

Sequence Viewer

Length: 480 bp
ATGGCATCAAAAATCACGTACGATGCCGTTCCGAATCCGAAAACGGACTCCGCCGCGGAATCCGATGACGGGGAAGAATTGATGCTCGCGAGGAGAAGCGGGTGCTGTTTCTGGATCCCCTGCTTTGGATCCGAACCCTCCTCTCCCGACGGGTCGTGGGAGCGGATCCGTTCCACCGGTGACTGCGAGGGTCTGTGGTGGAACCGCGGCTGGAAGAAGATCCGGGAGTGGTCGGAGCTCCTTGCGGGTCCCAAGTGGAAGACCTTCATCCGGCGGTTCAACAAAAACAACCCCAGGCGGTCCGTGAAATTCCAATACGACCCCATGAGTTACGCCCTCAACTTCGACGAAGGACCGGGGCAGAACGGTAATTTGAGTGATGATTATTTGTACAGGGATTTCTCGTGCCGGTACGCGTCGATTCCGGCGTCGGCCAAGTCGTCGATGGATTTGAGTAACGACTCCCCCTCCTTGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

18.09

Weight (kDa)

5.56

Isoelectric Point (pI)

57.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017084)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01430 AT5G14895
fragaria_vesca FvH4_6g50470
malus_domestica MD09G1036100.v1.1 MD17G1037700.v1.1
prunus_persica Prupe.3G281600_v2.0.a1
pyrus_communis pycom111g02900 pycom17g03390
rosa_laevigata RLG00000021989
rosa_multiflora Rmu_sc0009938.1_g000004
rosa_rugosa Rorug02G0552000
rosa_samantha Rh2BG635400 Rh2DG647000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 163
AccII CGCG 4 cut(s) 56, 89, 207, 416
AclWI GGATC 7 cut(s) 109, 122, 123, 136, 160, 173, 214
AcoI YGGCCR 1 cut(s) 432
AcsI RAATTY 1 cut(s) 308
AcyI GRCGYC 1 cut(s) 428
AfaI GTAC 3 cut(s) 20, 392, 413
AfiI CCNNNNNNNGG 3 cut(s) 69, 125, 270
AflIII ACRYGT 1 cut(s) 414
AgeI ACCGGT 1 cut(s) 176
AgsI TTSAA 1 cut(s) 280
AjiI CACGTC 1 cut(s) 477
AjnI CCWGG 1 cut(s) 293
AluBI AGCT 1 cut(s) 238
AluI AGCT 1 cut(s) 238
Alw21I GWGCWC 1 cut(s) 240
AlwI GGATC 7 cut(s) 109, 122, 123, 136, 160, 173, 214
AoxI GGCC 1 cut(s) 432
ApoI RAATTY 1 cut(s) 308
AsiGI ACCGGT 1 cut(s) 176
Asp700I GAANNNNTTC 1 cut(s) 263
AspS9I GGNCC 3 cut(s) 248, 300, 353
AsuC2I CCSGG 2 cut(s) 224, 357
AsuHPI GGTGA 1 cut(s) 191
AvaII GGWCC 3 cut(s) 248, 300, 353
BamHI GGATCC 3 cut(s) 114, 128, 165
BanII GRGCYC 1 cut(s) 240
BauI CACGAG 1 cut(s) 403
BbsI GAAGAC 1 cut(s) 266
Bbv12I GWGCWC 1 cut(s) 240
BccI CCATC 1 cut(s) 439
BceAI ACGGC 1 cut(s) 11
BciT130I CCWGG 1 cut(s) 295
BcnI CCSGG 2 cut(s) 224, 357
BisI GCNGC 2 cut(s) 54, 208
BlsI GCNGC 2 cut(s) 55, 209
Bme1390I CCNGG 3 cut(s) 224, 295, 357
Bme18I GGWCC 3 cut(s) 248, 300, 353
BmgBI CACGTC 1 cut(s) 477
BmgT120I GGNCC 3 cut(s) 248, 300, 353
BmiI GGNNCC 6 cut(s) 116, 130, 167, 203, 249, 250
BmrFI CCNGG 3 cut(s) 224, 295, 357
BmsI GCATC 3 cut(s) 13, 14, 72
BpiI GAAGAC 1 cut(s) 266
BpuMI CCSGG 2 cut(s) 224, 357
BsaAI YACGTR 1 cut(s) 18
BsaHI GRCGYC 1 cut(s) 428
BsaJI CCNNGG 4 cut(s) 54, 205, 293, 356
BsaWI WCCGGW 1 cut(s) 176
BsaXI ACNNNNNCTCC 1 cut(s) 452
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 125, 270
Bse118I RCCGGY 2 cut(s) 176, 408
BseBI CCWGG 1 cut(s) 295
BseDI CCNNGG 4 cut(s) 54, 205, 293, 356
BseGI GGATG 1 cut(s) 267
BseLI CCNNNNNNNGG 3 cut(s) 69, 125, 270
BseRI GAGGAG 2 cut(s) 106, 130
Bsh1236I CGCG 4 cut(s) 56, 89, 207, 416
BshFI GGCC 1 cut(s) 434
BshTI ACCGGT 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 240
BsiSI CCGG 6 cut(s) 177, 223, 271, 356, 409, 425
BsiWI CGTACG 1 cut(s) 18
BslFI GGGAC 1 cut(s) 234
BslI CCNNNNNNNGG 3 cut(s) 69, 125, 270
BsmFI GGGAC 1 cut(s) 234
BsnI GGCC 1 cut(s) 434
Bsp1286I GDGCHC 1 cut(s) 240
Bsp1407I TGTACA 1 cut(s) 390
Bsp143I GATC 4 cut(s) 114, 128, 165, 219
Bsp68I TCGCGA 1 cut(s) 89
BspANI GGCC 1 cut(s) 434
BspFNI CGCG 4 cut(s) 56, 89, 207, 416
BspLI GGNNCC 6 cut(s) 116, 130, 167, 203, 249, 250
BspPI GGATC 7 cut(s) 109, 122, 123, 136, 160, 173, 214
BsrBI CCGCTC 1 cut(s) 163
BsrFI RCCGGY 2 cut(s) 176, 408
BsrGI TGTACA 1 cut(s) 390
BssAI RCCGGY 2 cut(s) 176, 408
BssECI CCNNGG 4 cut(s) 54, 205, 293, 356
BssMI GATC 4 cut(s) 114, 128, 165, 219
BssNI GRCGYC 1 cut(s) 428
BssSI CACGAG 1 cut(s) 403
Bst2BI CACGAG 1 cut(s) 403
Bst2UI CCWGG 1 cut(s) 295
Bst4CI ACNGT 1 cut(s) 368
BstACI GRCGYC 1 cut(s) 428
BstAUI TGTACA 1 cut(s) 390
BstBAI YACGTR 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 87
BstDSI CCRYGG 2 cut(s) 54, 205
BstF5I GGATG 1 cut(s) 267
BstFNI CGCG 4 cut(s) 56, 89, 207, 416
BstKTI GATC 4 cut(s) 117, 131, 168, 222
BstMBI GATC 4 cut(s) 114, 128, 165, 219
BstNI CCWGG 1 cut(s) 295
BstSCI CCNGG 3 cut(s) 222, 293, 355
BstUI CGCG 4 cut(s) 56, 89, 207, 416
BstV2I GAAGAC 1 cut(s) 266
BstX2I RGATCY 4 cut(s) 114, 128, 165, 219
BstYI RGATCY 4 cut(s) 114, 128, 165, 219
BsuRI GGCC 1 cut(s) 434
BtgI CCRYGG 2 cut(s) 54, 205
BtrI CACGTC 1 cut(s) 477
BtsCI GGATG 1 cut(s) 267
BtuMI TCGCGA 1 cut(s) 89
Cac8I GCNNGC 1 cut(s) 87
Cfr10I RCCGGY 2 cut(s) 176, 408
Cfr13I GGNCC 3 cut(s) 248, 300, 353
Cfr42I CCGCGG 2 cut(s) 57, 208
CpoI CGGWCCG 1 cut(s) 300
CseI GACGC 2 cut(s) 405, 417
Csp6I GTAC 3 cut(s) 19, 391, 412
CspAI ACCGGT 1 cut(s) 176
CspI CGGWCCG 1 cut(s) 300
CviAII CATG 1 cut(s) 325
CviJI RGCY 3 cut(s) 210, 238, 434
CviKI_1 RGCY 3 cut(s) 210, 238, 434
CviQI GTAC 3 cut(s) 19, 391, 412
DpnI GATC 4 cut(s) 116, 130, 167, 221
DpnII GATC 4 cut(s) 114, 128, 165, 219
EaeI YGGCCR 1 cut(s) 432
EciI GGCGGA 1 cut(s) 40
Ecl136II GAGCTC 1 cut(s) 238
Eco24I GRGCYC 1 cut(s) 240
Eco47I GGWCC 3 cut(s) 248, 300, 353
Eco53kI GAGCTC 1 cut(s) 238
EcoICRI GAGCTC 1 cut(s) 238
EcoO109I RGGNCCY 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 293
EcoT38I GRGCYC 1 cut(s) 240
FaeI CATG 1 cut(s) 328
FaiI YATR 1 cut(s) 326
FaqI GGGAC 1 cut(s) 234
FatI CATG 1 cut(s) 324
FauI CCCGC 2 cut(s) 92, 238
Fnu4HI GCNGC 2 cut(s) 54, 208
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 2 cut(s) 54, 208
GluI GCNGC 2 cut(s) 54, 208
HaeIII GGCC 1 cut(s) 434
HapII CCGG 6 cut(s) 177, 223, 271, 356, 409, 425
HgaI GACGC 2 cut(s) 405, 417
Hin1I GRCGYC 1 cut(s) 428
Hin1II CATG 1 cut(s) 328
HinfI GANTC 5 cut(s) 34, 47, 59, 421, 461
HpaII CCGG 6 cut(s) 177, 223, 271, 356, 409, 425
HphI GGTGA 1 cut(s) 191
Hpy188I TCNGA 5 cut(s) 33, 39, 64, 133, 235
Hpy188III TCNNGA 3 cut(s) 88, 112, 146
Hpy99I CGWCG 5 cut(s) 152, 350, 421, 433, 445
HpyAV CCTTC 2 cut(s) 274, 344
HpyCH4III ACNGT 1 cut(s) 368
HpyCH4IV ACGT 2 cut(s) 17, 476
HpySE526I ACGT 2 cut(s) 17, 476
Hsp92I GRCGYC 1 cut(s) 428
Hsp92II CATG 1 cut(s) 328
KflI GGGWCCC 1 cut(s) 248
KspI CCGCGG 2 cut(s) 57, 208
Kzo9I GATC 4 cut(s) 114, 128, 165, 219
LmnI GCTCC 3 cut(s) 160, 235, 243
LweI GCATC 3 cut(s) 13, 14, 72
MaeII ACGT 2 cut(s) 17, 476
MaeIII GTNAC 3 cut(s) 179, 329, 455
MalI GATC 4 cut(s) 116, 130, 167, 221
MbiI CCGCTC 1 cut(s) 163
MboI GATC 4 cut(s) 114, 128, 165, 219
MboII GAAGA 4 cut(s) 86, 226, 229, 271
MflI RGATCY 4 cut(s) 114, 128, 165, 219
MhlI GDGCHC 1 cut(s) 240
MluCI AATT 3 cut(s) 77, 308, 370
MluI ACGCGT 1 cut(s) 414
MlyI GAGTC 2 cut(s) 41, 455
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 6 cut(s) 84, 148, 151, 181, 347, 478
MroXI GAANNNNTTC 1 cut(s) 263
MspA1I CMGCKG 2 cut(s) 56, 207
MspI CCGG 6 cut(s) 177, 223, 271, 356, 409, 425
MspR9I CCNGG 3 cut(s) 224, 295, 357
MvaI CCWGG 1 cut(s) 295
MvnI CGCG 4 cut(s) 56, 89, 207, 416
NciI CCSGG 2 cut(s) 224, 357
NdeII GATC 4 cut(s) 114, 128, 165, 219
NlaIII CATG 1 cut(s) 328
NlaIV GGNNCC 6 cut(s) 116, 130, 167, 203, 249, 250
NmuCI GTSAC 1 cut(s) 179
NruI TCGCGA 1 cut(s) 89
PcsI WCGNNNNNNNCGW 2 cut(s) 425, 437
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 3 cut(s) 34, 59, 421
Pfl23II CGTACG 1 cut(s) 18
PfoI TCCNGGA 1 cut(s) 222
PinAI ACCGGT 1 cut(s) 176
PkrI GCNGC 2 cut(s) 55, 209
PleI GAGTC 2 cut(s) 41, 455
PpsI GAGTC 2 cut(s) 41, 455
Ppu21I YACGTR 1 cut(s) 18
PpuMI RGGWCCY 1 cut(s) 248
Psp124BI GAGCTC 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 248
Psp6I CCWGG 1 cut(s) 293
PspGI CCWGG 1 cut(s) 293
PspLI CGTACG 1 cut(s) 18
PspN4I GGNNCC 6 cut(s) 116, 130, 167, 203, 249, 250
PspPI GGNCC 3 cut(s) 248, 300, 353
PspPPI RGGWCCY 1 cut(s) 248
PsuI RGATCY 4 cut(s) 114, 128, 165, 219
RruI TCGCGA 1 cut(s) 89
RsaI GTAC 3 cut(s) 20, 392, 413
RsaNI GTAC 3 cut(s) 19, 391, 412
Rsr2I CGGWCCG 1 cut(s) 300
RsrII CGGWCCG 1 cut(s) 300
SacI GAGCTC 1 cut(s) 240
SacII CCGCGG 2 cut(s) 57, 208
SatI GCNGC 2 cut(s) 54, 208
Sau3AI GATC 4 cut(s) 114, 128, 165, 219
Sau96I GGNCC 3 cut(s) 248, 300, 353
SchI GAGTC 2 cut(s) 41, 455
ScrFI CCNGG 3 cut(s) 224, 295, 357
SduI GDGCHC 1 cut(s) 240
SetI ASST 4 cut(s) 20, 240, 266, 479
SfaNI GCATC 3 cut(s) 13, 14, 72
Sfr303I CCGCGG 2 cut(s) 57, 208
SgrAI CRCCGGYG 1 cut(s) 176
SgrBI CCGCGG 2 cut(s) 57, 208
SinI GGWCC 3 cut(s) 248, 300, 353
Sse9I AATT 3 cut(s) 77, 308, 370
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 3 cut(s) 222, 293, 355
TaaI ACNGT 1 cut(s) 368
TaiI ACGT 2 cut(s) 20, 479
TaqI TCGA 3 cut(s) 345, 419, 443
TasI AATT 3 cut(s) 77, 308, 370
TatI WGTACW 1 cut(s) 390
TauI GCSGC 2 cut(s) 56, 210
TfiI GAWTC 3 cut(s) 34, 59, 421
TseFI GTSAC 1 cut(s) 179
Tsp45I GTSAC 1 cut(s) 179
TspDTI ATGAA 1 cut(s) 256
TspGWI ACGGA 3 cut(s) 59, 158, 292
VpaK11BI GGWCC 3 cut(s) 248, 300, 353
XapI RAATTY 1 cut(s) 308
XcmI CCANNNNNNNNNTGG 1 cut(s) 442
XmnI GAANNNNTTC 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.