Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

SuperScaffold_111

Orthogroup ID

OG0013505

Physical Location & Seq

Genomic Coordinates Reverse (-)

2115511 .. 2117544

Gene Structure 2034 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom111g03450.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2034 bp

ATGCAACTCGGCACAGCGTCTTCTTCCTTCTCTATTCCAGTTTTAACCCCCAACAAATCCCATAATTACACCGCGGTACCCTTCACCTTCAACCACCGCGAACCAAAACCCAGAACCTGTTTCCGACCCATTGGATTTTCATCTTCTTCGCCAACCAGAAATGTCTGGAGGAGAACCCCGCAGCTCAAAACGGCGCCGTCTCCCTCCTCCACGCCGTCCCACCAGAACCGCCGCCCCAGAAACCAGCAAGGTCCGTCGCATTTGGACCATAGCGTCGACATGGACGAGCTCTTGTCATCAATCGGACAGACCCAGAACGAGCTGGAACTGTACTCTCTAATGTCGGCCTACAAAGGCCGGCAGCTGTCGATTCGGTTCATGGTTTCGCTGCTCTCGCGGGAGTCTGATTGGCAGCGGTCACTCGCTATCCTCGATTGGATTAACGAGGAAGCTCTGTACACTCCCTCCGTGTTCGCTTACAATGTTGTGATACGAAACGTGCTTCGCGCCAAGCAGTGGGAGATTGCACACGGACTGTTCGACGAAATGCGCCAGCGAGCGCTGGCACCTGATAGGTACACTTATTCGACGCTGATTACTTCTTTCGGGAAGGCGGGTATGTTTGATTCGGCGCTCTCTTGGCTCCAGAAGATGGAGCAGGACCGTGTGCCCGGCGATCTTGTTCTGTACAGCAACTTGATTGAGCTTTCGCGTAAGTTGTGTGATTATTCCAAGGCAATTTCGATTTTTTCGAGGTTGAAGAGGTCGGGGATTATGCCGGACCTTGTGGCTTACAATTCGATGATCAATGTGTTTGGGAAAGCTAGGTTGTTTAGGGAAGCTCGGCTCCTTATTAAGGAGATGAGGGCGGTTGGTGTTTTGCCAGATACAGTTAGTTATTCGACGCTTTTGAGCATGTACATTGAGAACCAAAAGTTTGTTGAGGCGTTGTCTGTGTTCTCTGAGATGAACGAGGTTAAGTGTCCGCTTGATCTCACGACGTGCAATGTTATGATTGATGTTTACGGGCAGCTCGATATGGCTAAGGAAGCTGACAGGTTGTTTTGGGGCATGAGGAAAATGGGACTTGAACCCAATGTTGTTAGTTACAATACTCTTTTGAGGGTTTATGGCGATGCTGAGCTTTTCGGGGAAGCCATCCATCTTTTTCGATTGATGCAGAGAATGGATATTGAGCAGAATGTTGTCACATACAATACGATGATCAAGATTTATGGGAAGTCACTTGAACACGAAAAAGCTACGAATCTTGTGCAAGAAATGCAGCAGAGAGGGGTTCAGCCAAATGCAATTACATACTCAACGATTATATCTATATGGGGTAAGGCTGGGAAACTGGATCGGGCAGCAATGCTGTTTCAAAAGCTGAGGAGCTCTGGGGTTGAGATTGATCAGGTTCTTTATCAGACCATGATTGTGGCTTATGAGAGAGTAGGCTTGGTTGCTCATGCTAAGCGTTTACTTCATGAGCTCAAGCGCCCAGACAATATCCCTAGGGAGACTGCAATTACGATTCTTGCTAGAGCCGGTCGCATAGAAGAGGCTACATGGGTTTTCCGTCAAGCTTTTGAGGCTGGGGAGGTGAAGGATATATCGGTCTTTGGTTGCATGATTGATCTTTTTTCAAGAAACCGGAAGTACGCGAACTGCATTGAGGTGTTTGAAAAGATGAGAGTGGCAGGATACTTTCCTGCTTCCAATGTGATTGATCTAGTTCTCAATGCTTATGGAAAGTTACGGGAATTTGAGAAGGCAGATGCTGTATACAGTGAGATGCAGGAAGAAGGGTGTGTTTTCTCCGACGAAGTTCACTTCCAGATGCTTAGTCTTTACGGTGCAAGAAAAGATTTTAAGATGGTAGAGTCACTATTTGAGAGGCTGATCTCTGATCCCAACATCAACAAGAAAGAGTTACATCTCGTTGTTGCCAGCATCTACGATCGATCAAATAGACTTAACGATGCATCCCGGGTCATGAACAAGATGAATGAAAGGCGAATTATGATGTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016787 GO:0016788 GO:0031425 GO:0031967 GO:0031975 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

678

Amino Acids

77.71

Weight (kDa)

9.0

Isoelectric Point (pI)

39.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	155 - 203	2.4e-13	PPR repeat family
PPR_1	PF12854	188 - 219	2.8e-06	PPR repeat
PPR	PF01535	193 - 222	2.8e-06	PPR repeat
PPR_3	PF13812	215 - 274	2.7e-08	Pentatricopeptide repeat domain
PPR_long	PF17177	245 - 327	8e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	260 - 306	2.8e-10	PPR repeat family
PPR	PF01535	263 - 292	4.8e-06	PPR repeat
PPR_3	PF13812	284 - 344	6.2e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	295 - 342	5.7e-10	PPR repeat family
PPR_3	PF13812	330 - 379	5e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	331 - 376	2.4e-09	PPR repeat family
PPR_long	PF17177	357 - 515	8e-12	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	365 - 405	4.9e-08	PPR repeat family
TPR_24	PF23276	378 - 492	5.1e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	389 - 449	1.2e-11	Pentatricopeptide repeat domain
PPR_2	PF13041	401 - 445	1.6e-14	PPR repeat family
PPR	PF01535	403 - 433	1.4e-06	PPR repeat
PPR_1	PF12854	431 - 462	3.5e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0013505)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G39980
fragaria_vesca	FvH4_6g49520
malus_domestica	MD09G1044200.v1.1
prunus_persica	Prupe.3G273600_v2.0.a1
pyrus_communis	pycom111g03450
rosa_chinensis	RchiOBHm_Chr2g0169431
rosa_laevigata	RLG00000021863
rosa_multiflora	Rmu_co8342305.1_g000001 Rmu_sc0004665.1_g000014 Rmu_sc0004665.1_g000015 Rmu_sc0006806.1_g000040
rosa_roxburghii	Rroxscaffold_2G00082130 Rroxscaffold_3G00240790
rosa_samantha	Rh2AG611300 Rh2BG624900 Rh2CG593300 Rh2DG635500
rosa_wichuraiana	Rw2G050730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	272
Acc65I	GGTACC	1 cut(s)	76
AccB1I	GGYRCC	3 cut(s)	76, 193, 565
AccB7I	CCANNNNNTGG	2 cut(s)	516, 652
AccI	GTMKAC	2 cut(s)	276, 1785
AccII	CGCG	6 cut(s)	74, 99, 397, 507, 712, 1664
AclWI	GGATC	2 cut(s)	1368, 1904
AcsI	RAATTY	1 cut(s)	1763
AcyI	GRCGYC	1 cut(s)	194
AdeI	CACNNNGTG	1 cut(s)	1002
AfaI	GTAC	7 cut(s)	78, 332, 458, 578, 689, 920, 1661
AfeI	AGCGCT	1 cut(s)	561
AfiI	CCNNNNNNNGG	3 cut(s)	516, 652, 856
AgsI	TTSAA	7 cut(s)	91, 760, 1091, 1250, 1382, 1647, 1685
AjiI	CACGTC	1 cut(s)	1002
Alw21I	GWGCWC	3 cut(s)	291, 1397, 1494
Alw26I	GTCTC	2 cut(s)	204, 1514
AlwI	GGATC	2 cut(s)	1368, 1904
AlwNI	CAGNNNCTG	2 cut(s)	117, 1781
Ama87I	CYCGRG	1 cut(s)	1989
Aor51HI	AGCGCT	1 cut(s)	561
AoxI	GGCC	2 cut(s)	345, 355
ApeKI	GCWGC	7 cut(s)	181, 361, 388, 412, 1030, 1285, 1367
ApoI	RAATTY	1 cut(s)	1763
Asp718I	GGTACC	1 cut(s)	76
AspA2I	CCTAGG	1 cut(s)	1514
AspLEI	GCGC	6 cut(s)	196, 509, 552, 562, 634, 1500
AspS9I	GGNCC	4 cut(s)	251, 265, 661, 781
AsuC2I	CCSGG	3 cut(s)	672, 1990, 1991
AsuHPI	GGTGA	2 cut(s)	76, 1615
AvaI	CYCGRG	1 cut(s)	1989
AvaII	GGWCC	4 cut(s)	251, 265, 661, 781
AvrII	CCTAGG	1 cut(s)	1514
BaeGI	GKGCMC	1 cut(s)	672
BaeI	ACNNNNGTAYC	2 cut(s)	60, 93
BanI	GGYRCC	3 cut(s)	76, 193, 565
BanII	GRGCYC	3 cut(s)	291, 1397, 1494
BbsI	GAAGAC	1 cut(s)	12
Bbv12I	GWGCWC	3 cut(s)	291, 1397, 1494
BbvCI	CCTCAGC	1 cut(s)	1388
BbvI	GCAGC	7 cut(s)	193, 373, 375, 424, 1042, 1297, 1379
BccI	CCATC	4 cut(s)	646, 1166, 1170, 1870
BceAI	ACGGC	3 cut(s)	181, 199, 207
BcgI	CGANNNNNNTGC	2 cut(s)	1255, 1289
BciVI	GTATCC	1 cut(s)	1697
BclI	TGATCA	3 cut(s)	804, 1224, 1411
BcnI	CCSGG	3 cut(s)	672, 1990, 1991
BcoDI	GTCTC	2 cut(s)	204, 1514
BfaI	CTAG	4 cut(s)	825, 1515, 1542, 1733
BfoI	RGCGCY	4 cut(s)	197, 563, 635, 1501
BfuI	GTATCC	1 cut(s)	1697
BisI	GCNGC	8 cut(s)	182, 232, 362, 389, 413, 1031, 1286, 1368
BlnI	CCTAGG	1 cut(s)	1514
BlpI	GCTNAGC	2 cut(s)	1140, 1473
BlsI	GCNGC	8 cut(s)	183, 233, 363, 390, 414, 1032, 1287, 1369
Bme1390I	CCNGG	3 cut(s)	672, 1990, 1991
Bme18I	GGWCC	4 cut(s)	251, 265, 661, 781
BmeT110I	CYCGRG	1 cut(s)	1989
BmgBI	CACGTC	1 cut(s)	1002
BmgT120I	GGNCC	4 cut(s)	251, 265, 661, 781
BmiI	GGNNCC	5 cut(s)	78, 195, 567, 644, 848
BmrFI	CCNGG	3 cut(s)	672, 1990, 1991
BmsI	GCATC	8 cut(s)	1126, 1167, 1768, 1785, 1830, 1962, 1972, 1994
BpiI	GAAGAC	1 cut(s)	12
BpmI	CTGGAG	2 cut(s)	187, 629
Bpu10I	CCTNAGC	2 cut(s)	1044, 1388
Bpu1102I	GCTNAGC	2 cut(s)	1140, 1473
BpuEI	CTTGAG	1 cut(s)	1478
BpuMI	CCSGG	3 cut(s)	672, 1990, 1991
Bsa29I	ATCGAT	1 cut(s)	1963
BsaBI	GATNNNNATC	1 cut(s)	139
BsaHI	GRCGYC	1 cut(s)	194
BsaJI	CCNNGG	4 cut(s)	72, 732, 1514, 1989
BsaWI	WCCGGW	1 cut(s)	1653
BsaXI	ACNNNNNCTCC	2 cut(s)	449, 479
Bsc4I	CCNNNNNNNGG	3 cut(s)	516, 652, 856
Bse118I	RCCGGY	2 cut(s)	357, 1547
Bse1I	ACTGG	2 cut(s)	38, 1362
Bse3DI	GCAATG	2 cut(s)	1012, 1377
Bse8I	GATNNNNATC	1 cut(s)	139
BseCI	ATCGAT	1 cut(s)	1963
BseDI	CCNNGG	4 cut(s)	72, 732, 1514, 1989
BseGI	GGATG	2 cut(s)	1158, 1985
BseJI	GATNNNNATC	1 cut(s)	139
BseLI	CCNNNNNNNGG	3 cut(s)	516, 652, 856
BseMI	GCAATG	2 cut(s)	1012, 1377
BseMII	CTCAG	3 cut(s)	954, 1131, 1379
BseNI	ACTGG	2 cut(s)	38, 1362
BseRI	GAGGAG	3 cut(s)	184, 196, 1405
BseSI	GKGCMC	1 cut(s)	672
BseXI	GCAGC	7 cut(s)	193, 373, 375, 424, 1042, 1297, 1379
BseYI	CCCAGC	2 cut(s)	1349, 1595
Bsh1236I	CGCG	6 cut(s)	74, 99, 397, 507, 712, 1664
Bsh1285I	CGRYCG	2 cut(s)	1552, 1963
BshFI	GGCC	2 cut(s)	347, 357
BshNI	GGYRCC	3 cut(s)	76, 193, 565
BshVI	ATCGAT	1 cut(s)	1963
BsiEI	CGRYCG	2 cut(s)	1552, 1963
BsiHKAI	GWGCWC	3 cut(s)	291, 1397, 1494
BsiHKCI	CYCGRG	1 cut(s)	1989
BsiSI	CCGG	6 cut(s)	358, 672, 779, 1548, 1654, 1990
BslFI	GGGAC	2 cut(s)	202, 1098
BslI	CCNNNNNNNGG	3 cut(s)	516, 652, 856
BsmAI	GTCTC	2 cut(s)	204, 1514
BsmBI	CGTCTC	1 cut(s)	204
BsmFI	GGGAC	2 cut(s)	202, 1098
BsnI	GGCC	2 cut(s)	347, 357
BsoBI	CYCGRG	1 cut(s)	1989
Bsp1286I	GDGCHC	4 cut(s)	291, 672, 1397, 1494
Bsp1407I	TGTACA	3 cut(s)	456, 687, 918
Bsp1720I	GCTNAGC	2 cut(s)	1140, 1473
BspANI	GGCC	2 cut(s)	347, 357
BspCNI	CTCAG	3 cut(s)	955, 1132, 1380
BspDI	ATCGAT	1 cut(s)	1963
BspFNI	CGCG	6 cut(s)	74, 99, 397, 507, 712, 1664
BspHI	TCATGA	2 cut(s)	1486, 1995
BspLI	GGNNCC	5 cut(s)	78, 195, 567, 644, 848
BspPI	GGATC	2 cut(s)	1368, 1904
BspT107I	GGYRCC	3 cut(s)	76, 193, 565
BsrDI	GCAATG	2 cut(s)	1012, 1377
BsrFI	RCCGGY	2 cut(s)	357, 1547
BsrGI	TGTACA	3 cut(s)	456, 687, 918
BsrI	ACTGG	2 cut(s)	38, 1362
BssAI	RCCGGY	2 cut(s)	357, 1547
BssECI	CCNNGG	4 cut(s)	72, 732, 1514, 1989
BssNAI	GTATAC	1 cut(s)	1786
BssNI	GRCGYC	1 cut(s)	194
BssT1I	CCWWGG	2 cut(s)	732, 1514
Bst1107I	GTATAC	1 cut(s)	1786
Bst4CI	ACNGT	6 cut(s)	330, 537, 665, 892, 1790, 1856
Bst6I	CTCTTC	2 cut(s)	755, 1554
BstACI	GRCGYC	1 cut(s)	194
BstAPI	GCANNNNNTGC	1 cut(s)	1282
BstAUI	TGTACA	3 cut(s)	456, 687, 918
BstC8I	GCNNGC	5 cut(s)	359, 554, 558, 564, 1951
BstDEI	CTNAG	6 cut(s)	963, 1044, 1140, 1388, 1473, 1844
BstDSI	CCRYGG	1 cut(s)	72
BstENI	CCTNNNNNAGG	1 cut(s)	854
BstF5I	GGATG	2 cut(s)	1158, 1985
BstFNI	CGCG	6 cut(s)	74, 99, 397, 507, 712, 1664
BstH2I	RGCGCY	4 cut(s)	197, 563, 635, 1501
BstHHI	GCGC	6 cut(s)	196, 509, 552, 562, 634, 1500
BstMAI	GTCTC	2 cut(s)	204, 1514
BstMCI	CGRYCG	2 cut(s)	1552, 1963
BstMWI	GCNNNNNNNGC	5 cut(s)	394, 640, 1049, 1282, 1592
BstNSI	RCATGY	1 cut(s)	919
BstSCI	CCNGG	3 cut(s)	670, 1988, 1989
BstSLI	GKGCMC	1 cut(s)	672
BstUI	CGCG	6 cut(s)	74, 99, 397, 507, 712, 1664
BstV1I	GCAGC	7 cut(s)	193, 373, 375, 424, 1042, 1297, 1379
BstV2I	GAAGAC	1 cut(s)	12
BstXI	CCANNNNNNTGG	1 cut(s)	1438
BstZ17I	GTATAC	1 cut(s)	1786
Bsu15I	ATCGAT	1 cut(s)	1963
BsuI	GTATCC	1 cut(s)	1697
BsuRI	GGCC	2 cut(s)	347, 357
BsuTUI	ATCGAT	1 cut(s)	1963
BtgI	CCRYGG	1 cut(s)	72
BtgZI	GCGATG	1 cut(s)	1149
BtrI	CACGTC	1 cut(s)	1002
BtsCI	GGATG	2 cut(s)	1158, 1985
BtsI	GCAGTG	1 cut(s)	521
BtsIMutI	CAGTG	2 cut(s)	521, 1795
Cac8I	GCNNGC	5 cut(s)	359, 554, 558, 564, 1951
CaiI	CAGNNNCTG	2 cut(s)	117, 1781
CciI	TCATGA	2 cut(s)	1486, 1995
CfoI	GCGC	6 cut(s)	196, 509, 552, 562, 634, 1500
Cfr10I	RCCGGY	2 cut(s)	357, 1547
Cfr13I	GGNCC	4 cut(s)	251, 265, 661, 781
Cfr42I	CCGCGG	1 cut(s)	75
Cfr9I	CCCGGG	1 cut(s)	1989
ClaI	ATCGAT	1 cut(s)	1963
CseI	GACGC	4 cut(s)	6, 262, 598, 913
Csp6I	GTAC	7 cut(s)	77, 331, 457, 577, 688, 919, 1660
CviQI	GTAC	7 cut(s)	77, 331, 457, 577, 688, 919, 1660
DdeI	CTNAG	6 cut(s)	963, 1044, 1140, 1388, 1473, 1844
DinI	GGCGCC	1 cut(s)	195
DraIII	CACNNNGTG	1 cut(s)	1002
DrdI	GACNNNNNNGTC	1 cut(s)	272
DseDI	GACNNNNNNGTC	1 cut(s)	272
Eam1104I	CTCTTC	2 cut(s)	755, 1554
EarI	CTCTTC	2 cut(s)	755, 1554
Ecl136II	GAGCTC	3 cut(s)	289, 1395, 1492
Eco130I	CCWWGG	2 cut(s)	732, 1514
Eco24I	GRGCYC	3 cut(s)	291, 1397, 1494
Eco47I	GGWCC	4 cut(s)	251, 265, 661, 781
Eco47III	AGCGCT	1 cut(s)	561
Eco53kI	GAGCTC	3 cut(s)	289, 1395, 1492
Eco88I	CYCGRG	1 cut(s)	1989
EcoICRI	GAGCTC	3 cut(s)	289, 1395, 1492
EcoNI	CCTNNNNNAGG	1 cut(s)	854
EcoT14I	CCWWGG	2 cut(s)	732, 1514
EcoT22I	ATGCAT	1 cut(s)	1987
EcoT38I	GRGCYC	3 cut(s)	291, 1397, 1494
EgeI	GGCGCC	1 cut(s)	195
EheI	GGCGCC	1 cut(s)	195
ErhI	CCWWGG	2 cut(s)	732, 1514
Esp3I	CGTCTC	1 cut(s)	204
FaqI	GGGAC	2 cut(s)	202, 1098
FauI	CCCGC	3 cut(s)	186, 390, 607
FbaI	TGATCA	3 cut(s)	804, 1224, 1411
FblI	GTMKAC	2 cut(s)	276, 1785
Fnu4HI	GCNGC	8 cut(s)	182, 232, 362, 389, 413, 1031, 1286, 1368
FokI	GGATG	2 cut(s)	1145, 1972
FriOI	GRGCYC	3 cut(s)	291, 1397, 1494
Fsp4HI	GCNGC	8 cut(s)	182, 232, 362, 389, 413, 1031, 1286, 1368
FspBI	CTAG	4 cut(s)	825, 1515, 1542, 1733
GlaI	GCGC	6 cut(s)	195, 508, 551, 561, 633, 1499
GluI	GCNGC	8 cut(s)	182, 232, 362, 389, 413, 1031, 1286, 1368
GsaI	CCCAGC	2 cut(s)	1353, 1599
GsuI	CTGGAG	2 cut(s)	187, 629
HaeII	RGCGCY	4 cut(s)	197, 563, 635, 1501
HaeIII	GGCC	2 cut(s)	347, 357
HapII	CCGG	6 cut(s)	358, 672, 779, 1548, 1654, 1990
HgaI	GACGC	4 cut(s)	6, 262, 598, 913
HhaI	GCGC	6 cut(s)	196, 509, 552, 562, 634, 1500
Hin1I	GRCGYC	1 cut(s)	194
Hin6I	GCGC	6 cut(s)	194, 507, 550, 560, 632, 1498
HinP1I	GCGC	6 cut(s)	194, 507, 550, 560, 632, 1498
HincII	GTYRAC	1 cut(s)	277
HindII	GTYRAC	1 cut(s)	277
HindIII	AAGCTT	1 cut(s)	1584
HinfI	GANTC	6 cut(s)	370, 401, 626, 1267, 1534, 1883
HpaII	CCGG	6 cut(s)	358, 672, 779, 1548, 1654, 1990
HphI	GGTGA	2 cut(s)	76, 1615
Hpy166II	GTNNAC	7 cut(s)	277, 459, 579, 1024, 1481, 1786, 1831
Hpy188I	TCNGA	7 cut(s)	125, 305, 406, 964, 1428, 1822, 1909
Hpy8I	GTNNAC	7 cut(s)	277, 459, 579, 1024, 1481, 1786, 1831
Hpy99I	CGWCG	7 cut(s)	259, 278, 545, 592, 907, 1003, 1826
HpyAV	CCTTC	7 cut(s)	37, 91, 97, 604, 1600, 1765, 1799
HpyCH4III	ACNGT	6 cut(s)	330, 537, 665, 892, 1790, 1856
HpyCH4IV	ACGT	2 cut(s)	498, 1001
HpyF10VI	GCNNNNNNNGC	5 cut(s)	394, 640, 1049, 1282, 1592
HpyF3I	CTNAG	6 cut(s)	963, 1044, 1140, 1388, 1473, 1844
HpySE526I	ACGT	2 cut(s)	498, 1001
Hsp92I	GRCGYC	1 cut(s)	194
HspAI	GCGC	6 cut(s)	194, 507, 550, 560, 632, 1498
KasI	GGCGCC	1 cut(s)	193
KpnI	GGTACC	1 cut(s)	80
KroI	GCCGGC	1 cut(s)	357
KroNI	GCCGGC	1 cut(s)	359
Ksp22I	TGATCA	3 cut(s)	804, 1224, 1411
KspI	CCGCGG	1 cut(s)	75
LmnI	GCTCC	4 cut(s)	648, 655, 852, 1392
Lsp1109I	GCAGC	7 cut(s)	193, 373, 375, 424, 1042, 1297, 1379
LweI	GCATC	8 cut(s)	1126, 1167, 1768, 1785, 1830, 1962, 1972, 1994
MaeI	CTAG	4 cut(s)	825, 1515, 1542, 1733
MaeII	ACGT	2 cut(s)	498, 1001
MaeIII	GTNAC	7 cut(s)	417, 1106, 1207, 1242, 1755, 1884, 1932
MboII	GAAGA	8 cut(s)	12, 15, 135, 138, 661, 772, 1571, 1814
MhlI	GDGCHC	4 cut(s)	291, 672, 1397, 1494
MluCI	AATT	7 cut(s)	64, 738, 796, 1311, 1527, 1763, 2019
Mly113I	GGCGCC	1 cut(s)	194
MlyI	GAGTC	2 cut(s)	410, 1892
MmeI	TCCRAC	2 cut(s)	148, 1845
Mph1103I	ATGCAT	1 cut(s)	1987
MroNI	GCCGGC	1 cut(s)	357
MseI	TTAA	6 cut(s)	44, 441, 855, 978, 1872, 1977
MslI	CAYNNNNRTG	2 cut(s)	1436, 1676
MspA1I	CMGCKG	3 cut(s)	74, 364, 415
MspI	CCGG	6 cut(s)	358, 672, 779, 1548, 1654, 1990
MspR9I	CCNGG	3 cut(s)	672, 1990, 1991
MvnI	CGCG	6 cut(s)	74, 99, 397, 507, 712, 1664
MwoI	GCNNNNNNNGC	5 cut(s)	394, 640, 1049, 1282, 1592
NaeI	GCCGGC	1 cut(s)	359
NarI	GGCGCC	1 cut(s)	194
NciI	CCSGG	3 cut(s)	672, 1990, 1991
NgoMIV	GCCGGC	1 cut(s)	357
NlaIV	GGNNCC	5 cut(s)	78, 195, 567, 644, 848
NmeAIII	GCCGAG	1 cut(s)	823
NmuCI	GTSAC	4 cut(s)	417, 1207, 1242, 1884
NsiI	ATGCAT	1 cut(s)	1987
NspI	RCATGY	1 cut(s)	919
PagI	TCATGA	2 cut(s)	1486, 1995
PcsI	WCGNNNNNNNCGW	5 cut(s)	282, 429, 537, 749, 1032
PdiI	GCCGGC	1 cut(s)	359
PfeI	GAWTC	4 cut(s)	370, 626, 1267, 1534
PflMI	CCANNNNNTGG	2 cut(s)	516, 652
PkrI	GCNGC	8 cut(s)	183, 233, 363, 390, 414, 1032, 1287, 1369
Ple19I	CGATCG	1 cut(s)	1963
PleI	GAGTC	2 cut(s)	409, 1891
PluTI	GGCGCC	1 cut(s)	197
PpsI	GAGTC	2 cut(s)	409, 1891
Psp124BI	GAGCTC	3 cut(s)	291, 1397, 1494
PspFI	CCCAGC	2 cut(s)	1349, 1595
PspN4I	GGNNCC	5 cut(s)	78, 195, 567, 644, 848
PspPI	GGNCC	4 cut(s)	251, 265, 661, 781
PstNI	CAGNNNCTG	2 cut(s)	117, 1781
PvuI	CGATCG	1 cut(s)	1963
PvuII	CAGCTG	1 cut(s)	364
RsaI	GTAC	7 cut(s)	78, 332, 458, 578, 689, 920, 1661
RsaNI	GTAC	7 cut(s)	77, 331, 457, 577, 688, 919, 1660
RseI	CAYNNNNRTG	2 cut(s)	1436, 1676
SacI	GAGCTC	3 cut(s)	291, 1397, 1494
SacII	CCGCGG	1 cut(s)	75
SalI	GTCGAC	1 cut(s)	275
SaqAI	TTAA	6 cut(s)	44, 441, 855, 978, 1872, 1977
SatI	GCNGC	8 cut(s)	182, 232, 362, 389, 413, 1031, 1286, 1368
Sau96I	GGNCC	4 cut(s)	251, 265, 661, 781
SchI	GAGTC	2 cut(s)	410, 1892
ScrFI	CCNGG	3 cut(s)	672, 1990, 1991
SduI	GDGCHC	4 cut(s)	291, 672, 1397, 1494
SfaNI	GCATC	8 cut(s)	1126, 1167, 1768, 1785, 1830, 1962, 1972, 1994
SfoI	GGCGCC	1 cut(s)	195
Sfr303I	CCGCGG	1 cut(s)	75
SgrBI	CCGCGG	1 cut(s)	75
SinI	GGWCC	4 cut(s)	251, 265, 661, 781
SmaI	CCCGGG	1 cut(s)	1991
SmiMI	CAYNNNNRTG	2 cut(s)	1436, 1676
SmlI	CTYRAG	1 cut(s)	1493
SmoI	CTYRAG	1 cut(s)	1493
Sse9I	AATT	7 cut(s)	64, 738, 796, 1311, 1527, 1763, 2019
SspDI	GGCGCC	1 cut(s)	193
SspMI	CTAG	4 cut(s)	825, 1515, 1542, 1733
SstI	GAGCTC	3 cut(s)	291, 1397, 1494
StyD4I	CCNGG	3 cut(s)	670, 1988, 1989
StyI	CCWWGG	2 cut(s)	732, 1514
TaaI	ACNGT	6 cut(s)	330, 537, 665, 892, 1790, 1856
TaiI	ACGT	2 cut(s)	501, 1004
TaqII	GACCGA	1 cut(s)	1606
TasI	AATT	7 cut(s)	64, 738, 796, 1311, 1527, 1763, 2019
TatI	WGTACW	4 cut(s)	330, 456, 687, 918
TauI	GCSGC	1 cut(s)	234
TfiI	GAWTC	4 cut(s)	370, 626, 1267, 1534
Tru1I	TTAA	6 cut(s)	44, 441, 855, 978, 1872, 1977
Tru9I	TTAA	6 cut(s)	44, 441, 855, 978, 1872, 1977
TscAI	CASTG	2 cut(s)	521, 1795
TseFI	GTSAC	4 cut(s)	417, 1207, 1242, 1884
TseI	GCWGC	7 cut(s)	181, 361, 388, 412, 1030, 1285, 1367
Tsp45I	GTSAC	4 cut(s)	417, 1207, 1242, 1884
TspDTI	ATGAA	7 cut(s)	129, 367, 983, 1475, 2012, 2021, 2025
TspGWI	ACGGA	4 cut(s)	243, 457, 546, 1568
TspMI	CCCGGG	1 cut(s)	1989
TspRI	CASTG	2 cut(s)	521, 1795
Van91I	CCANNNNNTGG	2 cut(s)	516, 652
VpaK11BI	GGWCC	4 cut(s)	251, 265, 661, 781
XagI	CCTNNNNNAGG	1 cut(s)	854
XapI	RAATTY	1 cut(s)	1763
XceI	RCATGY	1 cut(s)	919
XmaI	CCCGGG	1 cut(s)	1989
XmaJI	CCTAGG	1 cut(s)	1514
XmiI	GTMKAC	2 cut(s)	276, 1785
XspI	CTAG	4 cut(s)	825, 1515, 1542, 1733
Zsp2I	ATGCAT	1 cut(s)	1987

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.