Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0013505

Physical Location & Seq

Genomic Coordinates Forward (+)

78348898 .. 78351647

Gene Structure 2750 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G050730.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2052 bp

ATGTGTACATTCTCGCTTTCTGCAACTTCAATGCAAGTATCTCCATGTCCAAGTCCTTCTTCTTCCTTCTCATTTCCATCACTACCCCCCACCAAACCCCACAATTACACCGCCACACCCTTCACCTTCACCACCCACAAACCCAGACTCCGCTCCCCCCTTATCTCCTCCGCCTCCAAAGATGTCTGGAGAAGAACACCACCCCACCCCAAAACGACGCCGTCCCATCCCTCCCAACCCTCCCCCCGCCACCGCCGCAAGCTCCAAGGCACCTCCTTCCTGGACCACAGCGTCGACATGGACGACCTCACCGCCTCAATCCGCGCCACCCAAAACGAGCACGACCTCAACTCCCTCCTCTCCCCCTACAAGTCCCGCAACCTCTCCATCCGCTTCATGGTCTCCCTCCTCTCCCGCGAGCCCGACTGGCGCCGCTCCCTCGCCATCCTCGACTGGATCAACGACGTCGCCCTATACTCTCCCTCCGTCTTCGCCTACAACGTCGTCATCCGCAACGTCCTCCGCGCCAAGCAGTGGCAAGTCGCCCACGGCCTGTTCGACGAAATGCGCCACAGAGCTCTGGCGCCGGACCGCTACACTTACTCCACTCTCATCACCGCGTTTGGGAAGTCCGGCATGTTCGACGCCGCGCTCTCGTGGCTGCAGAAGATGGAGGAGGACCGGGTGTCCGGGGACCTGGTGCTGTATAGCAACTTGATTGAGCTGTCTAGGAAACTGTGTGATTATAGCAAAGCAATTTCGATATTTTCGAGGCTGAAGAGGTTGGGGATAATGCCGGATTTGGTGGCTTATAACTCGATGATCAATGTGTTTGGTAAGGCAAAGTTGTTTAGGGAGGCCAGGGTTTTGCTGAAGGAGATGAGGGCGGTGGGGGTTTTGCCGGACACTGTTAGCTACTCGACGCTTTTGACTATGTACATTGAGAATGAGAAGTTTGTGGAGGCGTTGGCTGTGTTCTCGGAGATGAATGAGGTCAAGTGCCCGCTTGATCTCACCACGTGTAATATTATGATTGATGTTTATGGGCAGCTGGATATGGCGAAGGAGGCTGATAGGTTGTTTTGGAGCATGAGGAAGATGGGGCTTGAGCCGAATGTTGTTAGTTACAATACTCTTTTGAGGGTTTATGGCGATGCTGAGCTTTTCGGGGAAGCCATTCATCTTTTTCGAATGATGCAGAGAATGGATGTGGAGCAGAATGTTGTCACGTACAATACGATGATTAGGATTTATGGGAAGTCGCTTGAGCATGAAAAGGCTACAAATCTTGTGCAGGAAATGCAGAAGAGGGGGATTCAGCCGAATGCCATTACATACTCGACGATTATATCTATATGGGGTAGGGCTGGGAAATTGGACAGGGCGGCAATGCTGTTTCAAAAGCTGAGGAGCTCTGGGGTTGAGATTGATCAGGTTCTTTATCAGACAATGATTGTATCTTATGAGAGAGTAGGTTTGGTTGCTCATGCTAAGCGCTTGCTTCATGAGCTTAAGCGCCCAGACAATATCCCGAGGGAGACTGCAATTACCATTCTTGCTGGAGCCGGTCGTGTAGAAGAGGCGACATGGGTTTTCCGTCAGGCTTTTGAGGCTGGGGAGGTGAAGGATATAGCTGTGTTTGGTTGTATGATTGAGCTTTTCTCAAGAAACCGGAAGTATGCAAATTGCATTGAAGTGTTTGAAAATATGCGAGTGGCAGGATACTTTCCTGCTTCCCATGTGATTGGTCTAGTTCTCAACGCTTATGGAAAGTTACGGGAGTTTGAGAAGGCTGATGCTGTATATAGGGAGATGCAGGAAGAAGGGTGTGTTTTCTCTGATGAAATTCACTTCCAAATGCTCAGTCTTTATGGTGCAAGAAGGGACTTTAAGACAGTAGAGGCAATGTTTGAGAGGCTGGTCTCCGATCCCAACATCAACAAAAAGGAGTTGCATCTTGTTGTTGCAGGCATCTATGAAAGATCAAACAGACTTAATGATTCATACCGGATCATGAACAGGATGAATGAAAGTCGAGCTTTGAAATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016787 GO:0016788 GO:0031425 GO:0031967 GO:0031975 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

683

Amino Acids

78.22

Weight (kDa)

9.14

Isoelectric Point (pI)

38.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	161 - 210	2.8e-13	PPR repeat family
PPR	PF01535	199 - 228	1.2e-06	PPR repeat
PPR_3	PF13812	221 - 280	5e-08	Pentatricopeptide repeat domain
PPR_long	PF17177	251 - 334	5.6e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	263 - 295	5.3e-06	PPR repeat
PPR_2	PF13041	266 - 312	4.5e-11	PPR repeat family
PPR	PF01535	269 - 298	2.7e-06	PPR repeat
PPR_3	PF13812	290 - 350	5.3e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	301 - 347	4.1e-10	PPR repeat family
PPR_3	PF13812	336 - 385	2.9e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	337 - 383	2.4e-10	PPR repeat family
PPR_long	PF17177	360 - 520	1.2e-11	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	371 - 416	2.3e-11	PPR repeat family
PPR_3	PF13812	395 - 454	6e-12	Pentatricopeptide repeat domain
PPR_2	PF13041	407 - 451	6.4e-15	PPR repeat family
PPR	PF01535	409 - 439	5.1e-07	PPR repeat
PPR_1	PF12854	437 - 468	3e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013505)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G39980
fragaria_vesca	FvH4_6g49520
malus_domestica	MD09G1044200.v1.1
prunus_persica	Prupe.3G273600_v2.0.a1
pyrus_communis	pycom111g03450
rosa_chinensis	RchiOBHm_Chr2g0169431
rosa_laevigata	RLG00000021863
rosa_multiflora	Rmu_co8342305.1_g000001 Rmu_sc0004665.1_g000014 Rmu_sc0004665.1_g000015 Rmu_sc0006806.1_g000040
rosa_roxburghii	Rroxscaffold_2G00082130 Rroxscaffold_3G00240790
rosa_samantha	Rh2AG611300 Rh2BG624900 Rh2CG593300 Rh2DG635500
rosa_wichuraiana	Rw2G050730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	813
AasI	GACNNNNNNGTC	1 cut(s)	290
AatII	GACGTC	1 cut(s)	468
AccB1I	GGYRCC	3 cut(s)	269, 429, 583
AccB7I	CCANNNNNTGG	1 cut(s)	534
AccBSI	CCGCTC	2 cut(s)	153, 435
AccI	GTMKAC	1 cut(s)	294
AccII	CGCG	5 cut(s)	324, 417, 525, 620, 650
AclWI	GGATC	3 cut(s)	464, 1922, 2018
AcsI	RAATTY	1 cut(s)	1845
AcuI	CTGAAG	2 cut(s)	797, 893
AcvI	CACGTG	1 cut(s)	1020
AcyI	GRCGYC	5 cut(s)	218, 430, 465, 584, 645
AdeI	CACNNNGTG	1 cut(s)	1020
AfaI	GTAC	3 cut(s)	7, 938, 1232
AfeI	AGCGCT	1 cut(s)	1496
AfiI	CCNNNNNNNGG	3 cut(s)	397, 534, 624
AflII	CTTAAG	1 cut(s)	1511
AflIII	ACRYGT	1 cut(s)	1019
AgsI	TTSAA	5 cut(s)	30, 1400, 1694, 1703, 2044
AhdI	GACNNNNNGTC	1 cut(s)	685
AjnI	CCWGG	3 cut(s)	279, 696, 860
AjuI	GAANNNNNNNTTGG	2 cut(s)	43, 75
Alw21I	GWGCWC	3 cut(s)	342, 580, 1415
Alw26I	GTCTC	3 cut(s)	406, 1532, 1927
AlwI	GGATC	3 cut(s)	464, 1922, 2018
Ama87I	CYCGRG	1 cut(s)	1531
Aor51HI	AGCGCT	1 cut(s)	1496
AoxI	GGCC	2 cut(s)	550, 858
ApeKI	GCWGC	2 cut(s)	661, 1048
ApoI	RAATTY	1 cut(s)	1845
ArsI	GACNNNNNNTTYG	2 cut(s)	1849, 1881
Asp700I	GAANNNNTTC	1 cut(s)	1176
AspLEI	GCGC	8 cut(s)	326, 432, 527, 570, 586, 652, 1497, 1518
AspS9I	GGNCC	4 cut(s)	283, 589, 679, 694
AsuC2I	CCSGG	2 cut(s)	683, 691
AsuHPI	GGTGA	6 cut(s)	115, 121, 301, 607, 1006, 1633
AsuII	TTCGAA	1 cut(s)	1189
AvaI	CYCGRG	1 cut(s)	1531
AvaII	GGWCC	4 cut(s)	283, 589, 679, 694
BaeGI	GKGCMC	1 cut(s)	1004
BanI	GGYRCC	3 cut(s)	269, 429, 583
BanII	GRGCYC	3 cut(s)	423, 580, 1415
BauI	CACGAG	1 cut(s)	655
BbrPI	CACGTG	1 cut(s)	1020
BbsI	GAAGAC	1 cut(s)	481
Bbv12I	GWGCWC	3 cut(s)	342, 580, 1415
BbvCI	CCTCAGC	1 cut(s)	1406
BbvI	GCAGC	2 cut(s)	648, 1060
BccI	CCATC	6 cut(s)	85, 234, 395, 452, 664, 1093
BceAI	ACGGC	2 cut(s)	205, 565
BciT130I	CCWGG	3 cut(s)	281, 698, 862
BciVI	GTATCC	1 cut(s)	1715
BclI	TGATCA	2 cut(s)	822, 1429
BcnI	CCSGG	2 cut(s)	683, 691
BcoDI	GTCTC	3 cut(s)	406, 1532, 1927
BfaI	CTAG	2 cut(s)	729, 1751
BfmI	CTRYAG	1 cut(s)	662
BfoI	RGCGCY	4 cut(s)	433, 587, 1498, 1519
BfrI	CTTAAG	1 cut(s)	1511
BfuI	GTATCC	1 cut(s)	1715
BglI	GCCNNNNNGGC	1 cut(s)	427
BisI	GCNGC	6 cut(s)	256, 433, 648, 662, 1049, 1386
BlpI	GCTNAGC	2 cut(s)	1158, 1491
BlsI	GCNGC	6 cut(s)	257, 434, 649, 663, 1050, 1387
Bme1390I	CCNGG	5 cut(s)	281, 683, 691, 698, 862
Bme18I	GGWCC	4 cut(s)	283, 589, 679, 694
BmeRI	GACNNNNNGTC	1 cut(s)	685
BmeT110I	CYCGRG	1 cut(s)	1531
BmgT120I	GGNCC	4 cut(s)	283, 589, 679, 694
BmiI	GGNNCC	5 cut(s)	271, 431, 585, 695, 1564
BmrFI	CCNGG	5 cut(s)	281, 683, 691, 698, 862
BmsI	GCATC	6 cut(s)	1144, 1185, 1786, 1803, 1963, 1980
BpiI	GAAGAC	1 cut(s)	481
BplI	GAGNNNNNCTC	2 cut(s)	1646, 1678
BpmI	CTGGAG	2 cut(s)	208, 1581
Bpu10I	CCTNAGC	1 cut(s)	1406
Bpu1102I	GCTNAGC	2 cut(s)	1158, 1491
Bpu14I	TTCGAA	1 cut(s)	1189
BpuEI	CTTGAG	3 cut(s)	1127, 1286, 1648
BpuMI	CCSGG	2 cut(s)	683, 691
BsaAI	YACGTR	2 cut(s)	1020, 1230
BsaHI	GRCGYC	5 cut(s)	218, 430, 465, 584, 645
BsaI	GGTCTC	2 cut(s)	406, 1927
BsaJI	CCNNGG	5 cut(s)	265, 547, 690, 861, 1532
BsaWI	WCCGGW	2 cut(s)	1671, 2007
BsaXI	ACNNNNNCTCC	8 cut(s)	132, 162, 335, 365, 467, 497, 587, 617
Bsc4I	CCNNNNNNNGG	3 cut(s)	397, 534, 624
Bse118I	RCCGGY	1 cut(s)	1565
Bse1I	ACTGG	2 cut(s)	431, 458
Bse3DI	GCAATG	2 cut(s)	1395, 1911
BseBI	CCWGG	3 cut(s)	281, 698, 862
BseDI	CCNNGG	5 cut(s)	265, 547, 690, 861, 1532
BseGI	GGATG	6 cut(s)	226, 387, 444, 507, 1213, 2028
BseLI	CCNNNNNNNGG	3 cut(s)	397, 534, 624
BseMI	GCAATG	2 cut(s)	1395, 1911
BseMII	CTCAG	3 cut(s)	1149, 1397, 1876
BseNI	ACTGG	2 cut(s)	431, 458
BseRI	GAGGAG	5 cut(s)	157, 347, 398, 689, 1423
BseSI	GKGCMC	1 cut(s)	1004
BseXI	GCAGC	2 cut(s)	648, 1060
BseYI	CCCAGC	2 cut(s)	1367, 1613
BsgI	GTGCAG	1 cut(s)	1313
Bsh1236I	CGCG	5 cut(s)	324, 417, 525, 620, 650
Bsh1285I	CGRYCG	1 cut(s)	1570
BshFI	GGCC	2 cut(s)	552, 860
BshNI	GGYRCC	3 cut(s)	269, 429, 583
BsiEI	CGRYCG	1 cut(s)	1570
BsiHKAI	GWGCWC	3 cut(s)	342, 580, 1415
BsiHKCI	CYCGRG	1 cut(s)	1531
BsiSI	CCGG	9 cut(s)	587, 633, 682, 690, 797, 902, 1566, 1672, 2008
BslFI	GGGAC	4 cut(s)	208, 358, 707, 1898
BslI	CCNNNNNNNGG	3 cut(s)	397, 534, 624
BsmAI	GTCTC	3 cut(s)	406, 1532, 1927
BsmFI	GGGAC	4 cut(s)	208, 358, 707, 1898
BsmI	GAATGC	1 cut(s)	1330
BsnI	GGCC	2 cut(s)	552, 860
Bso31I	GGTCTC	2 cut(s)	406, 1927
BsoBI	CYCGRG	1 cut(s)	1531
Bsp119I	TTCGAA	1 cut(s)	1189
Bsp1286I	GDGCHC	5 cut(s)	342, 423, 580, 1004, 1415
Bsp1407I	TGTACA	2 cut(s)	5, 936
Bsp143I	GATC	7 cut(s)	456, 822, 1009, 1429, 1927, 1982, 2010
Bsp1720I	GCTNAGC	2 cut(s)	1158, 1491
BspANI	GGCC	2 cut(s)	552, 860
BspCNI	CTCAG	3 cut(s)	1150, 1398, 1875
BspFNI	CGCG	5 cut(s)	324, 417, 525, 620, 650
BspHI	TCATGA	3 cut(s)	1504, 2013, 2048
BspLI	GGNNCC	5 cut(s)	271, 431, 585, 695, 1564
BspMAI	CTGCAG	1 cut(s)	666
BspPI	GGATC	3 cut(s)	464, 1922, 2018
BspT104I	TTCGAA	1 cut(s)	1189
BspT107I	GGYRCC	3 cut(s)	269, 429, 583
BspTI	CTTAAG	1 cut(s)	1511
BspTNI	GGTCTC	2 cut(s)	406, 1927
BsrBI	CCGCTC	2 cut(s)	153, 435
BsrDI	GCAATG	2 cut(s)	1395, 1911
BsrFI	RCCGGY	1 cut(s)	1565
BsrGI	TGTACA	2 cut(s)	5, 936
BsrI	ACTGG	2 cut(s)	431, 458
BssAI	RCCGGY	1 cut(s)	1565
BssECI	CCNNGG	5 cut(s)	265, 547, 690, 861, 1532
BssMI	GATC	7 cut(s)	456, 822, 1009, 1429, 1927, 1982, 2010
BssNI	GRCGYC	5 cut(s)	218, 430, 465, 584, 645
BssSI	CACGAG	1 cut(s)	655
BssT1I	CCWWGG	1 cut(s)	265
Bst2BI	CACGAG	1 cut(s)	655
Bst2UI	CCWGG	3 cut(s)	281, 698, 862
Bst4CI	ACNGT	3 cut(s)	738, 910, 1897
Bst6I	CTCTTC	3 cut(s)	773, 1301, 1572
BstACI	GRCGYC	5 cut(s)	218, 430, 465, 584, 645
BstAFI	CTTAAG	1 cut(s)	1511
BstAPI	GCANNNNNTGC	1 cut(s)	1300
BstAUI	TGTACA	2 cut(s)	5, 936
BstBAI	YACGTR	2 cut(s)	1020, 1230
BstBI	TTCGAA	1 cut(s)	1189
BstC8I	GCNNGC	5 cut(s)	260, 419, 1004, 1499, 1969
BstDEI	CTNAG	4 cut(s)	1158, 1406, 1491, 1862
BstDSI	CCRYGG	1 cut(s)	547
BstF5I	GGATG	6 cut(s)	226, 387, 444, 507, 1213, 2028
BstFNI	CGCG	5 cut(s)	324, 417, 525, 620, 650
BstH2I	RGCGCY	4 cut(s)	433, 587, 1498, 1519
BstHHI	GCGC	8 cut(s)	326, 432, 527, 570, 586, 652, 1497, 1518
BstKTI	GATC	7 cut(s)	459, 825, 1012, 1432, 1930, 1985, 2013
BstMAI	GTCTC	3 cut(s)	406, 1532, 1927
BstMBI	GATC	7 cut(s)	456, 822, 1009, 1429, 1927, 1982, 2010
BstMCI	CGRYCG	1 cut(s)	1570
BstMWI	GCNNNNNNNGC	7 cut(s)	255, 427, 658, 1067, 1300, 1507, 1610
BstNI	CCWGG	3 cut(s)	281, 698, 862
BstNSI	RCATGY	1 cut(s)	640
BstSCI	CCNGG	5 cut(s)	279, 681, 689, 696, 860
BstSFI	CTRYAG	1 cut(s)	662
BstSLI	GKGCMC	1 cut(s)	1004
BstUI	CGCG	5 cut(s)	324, 417, 525, 620, 650
BstV1I	GCAGC	2 cut(s)	648, 1060
BstV2I	GAAGAC	1 cut(s)	481
BstXI	CCANNNNNNTGG	1 cut(s)	1745
BsuI	GTATCC	1 cut(s)	1715
BsuRI	GGCC	2 cut(s)	552, 860
BtgI	CCRYGG	1 cut(s)	547
BtgZI	GCGATG	1 cut(s)	1167
BtsCI	GGATG	6 cut(s)	226, 387, 444, 507, 1213, 2028
BtsI	GCAGTG	1 cut(s)	539
BtsIMutI	CAGTG	2 cut(s)	539, 906
Cac8I	GCNNGC	5 cut(s)	260, 419, 1004, 1499, 1969
CciI	TCATGA	3 cut(s)	1504, 2013, 2048
CfoI	GCGC	8 cut(s)	326, 432, 527, 570, 586, 652, 1497, 1518
Cfr10I	RCCGGY	1 cut(s)	1565
Cfr13I	GGNCC	4 cut(s)	283, 589, 679, 694
CpoI	CGGWCCG	1 cut(s)	589
CseI	GACGC	4 cut(s)	226, 280, 653, 931
CsiI	ACCWGGT	1 cut(s)	696
Csp6I	GTAC	3 cut(s)	6, 937, 1231
CspI	CGGWCCG	1 cut(s)	589
CviQI	GTAC	3 cut(s)	6, 937, 1231
DdeI	CTNAG	4 cut(s)	1158, 1406, 1491, 1862
DinI	GGCGCC	2 cut(s)	431, 585
DpnI	GATC	7 cut(s)	458, 824, 1011, 1431, 1929, 1984, 2012
DpnII	GATC	7 cut(s)	456, 822, 1009, 1429, 1927, 1982, 2010
DraIII	CACNNNGTG	1 cut(s)	1020
DrdI	GACNNNNNNGTC	1 cut(s)	290
DriI	GACNNNNNGTC	1 cut(s)	685
DseDI	GACNNNNNNGTC	1 cut(s)	290
Eam1104I	CTCTTC	3 cut(s)	773, 1301, 1572
Eam1105I	GACNNNNNGTC	1 cut(s)	685
EarI	CTCTTC	3 cut(s)	773, 1301, 1572
EciI	GGCGGA	1 cut(s)	160
Ecl136II	GAGCTC	2 cut(s)	578, 1413
Eco130I	CCWWGG	1 cut(s)	265
Eco24I	GRGCYC	3 cut(s)	423, 580, 1415
Eco31I	GGTCTC	2 cut(s)	406, 1927
Eco47I	GGWCC	4 cut(s)	283, 589, 679, 694
Eco47III	AGCGCT	1 cut(s)	1496
Eco53kI	GAGCTC	2 cut(s)	578, 1413
Eco57I	CTGAAG	2 cut(s)	797, 893
Eco72I	CACGTG	1 cut(s)	1020
Eco88I	CYCGRG	1 cut(s)	1531
EcoICRI	GAGCTC	2 cut(s)	578, 1413
EcoO109I	RGGNCCY	1 cut(s)	694
EcoRII	CCWGG	3 cut(s)	279, 696, 860
EcoT14I	CCWWGG	1 cut(s)	265
EcoT38I	GRGCYC	3 cut(s)	423, 580, 1415
EgeI	GGCGCC	2 cut(s)	431, 585
EheI	GGCGCC	2 cut(s)	431, 585
ErhI	CCWWGG	1 cut(s)	265
FalI	AAGNNNNNCTT	3 cut(s)	43, 75, 2023
FaqI	GGGAC	4 cut(s)	208, 358, 707, 1898
FauI	CCCGC	4 cut(s)	254, 383, 422, 1011
FbaI	TGATCA	2 cut(s)	822, 1429
FblI	GTMKAC	1 cut(s)	294
Fnu4HI	GCNGC	6 cut(s)	256, 433, 648, 662, 1049, 1386
FokI	GGATG	6 cut(s)	213, 374, 431, 494, 1220, 2035
FriOI	GRGCYC	3 cut(s)	423, 580, 1415
Fsp4HI	GCNGC	6 cut(s)	256, 433, 648, 662, 1049, 1386
FspBI	CTAG	2 cut(s)	729, 1751
GlaI	GCGC	8 cut(s)	325, 431, 526, 569, 585, 651, 1496, 1517
GluI	GCNGC	6 cut(s)	256, 433, 648, 662, 1049, 1386
GsaI	CCCAGC	2 cut(s)	1371, 1617
GsuI	CTGGAG	2 cut(s)	208, 1581
HaeII	RGCGCY	4 cut(s)	433, 587, 1498, 1519
HaeIII	GGCC	2 cut(s)	552, 860
HapII	CCGG	9 cut(s)	587, 633, 682, 690, 797, 902, 1566, 1672, 2008
HgaI	GACGC	4 cut(s)	226, 280, 653, 931
HhaI	GCGC	8 cut(s)	326, 432, 527, 570, 586, 652, 1497, 1518
Hin1I	GRCGYC	5 cut(s)	218, 430, 465, 584, 645
Hin6I	GCGC	8 cut(s)	324, 430, 525, 568, 584, 650, 1495, 1516
HinP1I	GCGC	8 cut(s)	324, 430, 525, 568, 584, 650, 1495, 1516
HincII	GTYRAC	1 cut(s)	295
HindII	GTYRAC	1 cut(s)	295
HinfI	GANTC	3 cut(s)	147, 1315, 2000
HpaII	CCGG	9 cut(s)	587, 633, 682, 690, 797, 902, 1566, 1672, 2008
HphI	GGTGA	6 cut(s)	115, 121, 301, 607, 1006, 1633
Hpy166II	GTNNAC	2 cut(s)	6, 295
Hpy188I	TCNGA	4 cut(s)	982, 1446, 1840, 1927
Hpy188III	TCNNGA	6 cut(s)	187, 1505, 1531, 1665, 2014, 2049
Hpy8I	GTNNAC	2 cut(s)	6, 295
Hpy99I	CGWCG	9 cut(s)	220, 296, 467, 470, 506, 563, 647, 925, 1345
HpyCH4III	ACNGT	3 cut(s)	738, 910, 1897
HpyCH4IV	ACGT	5 cut(s)	465, 501, 516, 1019, 1229
HpyF10VI	GCNNNNNNNGC	7 cut(s)	255, 427, 658, 1067, 1300, 1507, 1610
HpyF3I	CTNAG	4 cut(s)	1158, 1406, 1491, 1862
HpySE526I	ACGT	5 cut(s)	465, 501, 516, 1019, 1229
Hsp92I	GRCGYC	5 cut(s)	218, 430, 465, 584, 645
HspAI	GCGC	8 cut(s)	324, 430, 525, 568, 584, 650, 1495, 1516
KasI	GGCGCC	2 cut(s)	429, 583
Ksp22I	TGATCA	2 cut(s)	822, 1429
Kzo9I	GATC	7 cut(s)	456, 822, 1009, 1429, 1927, 1982, 2010
LmnI	GCTCC	7 cut(s)	158, 267, 440, 1086, 1213, 1410, 1562
Lsp1109I	GCAGC	2 cut(s)	648, 1060
LweI	GCATC	6 cut(s)	1144, 1185, 1786, 1803, 1963, 1980
MabI	ACCWGGT	1 cut(s)	696
MaeI	CTAG	2 cut(s)	729, 1751
MaeII	ACGT	5 cut(s)	465, 501, 516, 1019, 1229
MaeIII	GTNAC	3 cut(s)	1124, 1225, 1773
MalI	GATC	7 cut(s)	458, 824, 1011, 1431, 1929, 1984, 2012
MbiI	CCGCTC	2 cut(s)	153, 435
MboI	GATC	7 cut(s)	456, 822, 1009, 1429, 1927, 1982, 2010
MhlI	GDGCHC	5 cut(s)	342, 423, 580, 1004, 1415
MluCI	AATT	6 cut(s)	103, 756, 1373, 1545, 1684, 1845
Mly113I	GGCGCC	2 cut(s)	430, 584
MlyI	GAGTC	1 cut(s)	141
MroXI	GAANNNNTTC	1 cut(s)	1176
MseI	TTAA	3 cut(s)	1512, 1890, 1995
MslI	CAYNNNNRTG	1 cut(s)	1694
MspA1I	CMGCKG	1 cut(s)	1051
MspCI	CTTAAG	1 cut(s)	1511
MspI	CCGG	9 cut(s)	587, 633, 682, 690, 797, 902, 1566, 1672, 2008
MspR9I	CCNGG	5 cut(s)	281, 683, 691, 698, 862
Mva1269I	GAATGC	1 cut(s)	1330
MvaI	CCWGG	3 cut(s)	281, 698, 862
MvnI	CGCG	5 cut(s)	324, 417, 525, 620, 650
MwoI	GCNNNNNNNGC	7 cut(s)	255, 427, 658, 1067, 1300, 1507, 1610
NarI	GGCGCC	2 cut(s)	430, 584
NciI	CCSGG	2 cut(s)	683, 691
NdeII	GATC	7 cut(s)	456, 822, 1009, 1429, 1927, 1982, 2010
NlaIV	GGNNCC	5 cut(s)	271, 431, 585, 695, 1564
NmuCI	GTSAC	1 cut(s)	1225
NspI	RCATGY	1 cut(s)	640
NspV	TTCGAA	1 cut(s)	1189
PagI	TCATGA	3 cut(s)	1504, 2013, 2048
PcsI	WCGNNNNNNNCGW	6 cut(s)	300, 447, 498, 555, 767, 1235
PctI	GAATGC	1 cut(s)	1330
PdmI	GAANNNNTTC	1 cut(s)	1176
PfeI	GAWTC	2 cut(s)	1315, 2000
PflFI	GACNNNGTC	1 cut(s)	220
PflMI	CCANNNNNTGG	1 cut(s)	534
PfoI	TCCNGGA	1 cut(s)	279
PkrI	GCNGC	6 cut(s)	257, 434, 649, 663, 1050, 1387
PleI	GAGTC	1 cut(s)	141
PluTI	GGCGCC	2 cut(s)	433, 587
PmaCI	CACGTG	1 cut(s)	1020
PmlI	CACGTG	1 cut(s)	1020
PpsI	GAGTC	1 cut(s)	141
Ppu21I	YACGTR	2 cut(s)	1020, 1230
PpuMI	RGGWCCY	1 cut(s)	694
PsiI	TTATAA	1 cut(s)	813
Psp124BI	GAGCTC	2 cut(s)	580, 1415
Psp5II	RGGWCCY	1 cut(s)	694
Psp6I	CCWGG	3 cut(s)	279, 696, 860
PspCI	CACGTG	1 cut(s)	1020
PspFI	CCCAGC	2 cut(s)	1367, 1613
PspGI	CCWGG	3 cut(s)	279, 696, 860
PspN4I	GGNNCC	5 cut(s)	271, 431, 585, 695, 1564
PspPI	GGNCC	4 cut(s)	283, 589, 679, 694
PspPPI	RGGWCCY	1 cut(s)	694
PstI	CTGCAG	1 cut(s)	666
PsyI	GACNNNGTC	1 cut(s)	220
PvuII	CAGCTG	1 cut(s)	1051
RsaI	GTAC	3 cut(s)	7, 938, 1232
RsaNI	GTAC	3 cut(s)	6, 937, 1231
RseI	CAYNNNNRTG	1 cut(s)	1694
Rsr2I	CGGWCCG	1 cut(s)	589
RsrII	CGGWCCG	1 cut(s)	589
SacI	GAGCTC	2 cut(s)	580, 1415
SalI	GTCGAC	1 cut(s)	293
SaqAI	TTAA	3 cut(s)	1512, 1890, 1995
SatI	GCNGC	6 cut(s)	256, 433, 648, 662, 1049, 1386
Sau3AI	GATC	7 cut(s)	456, 822, 1009, 1429, 1927, 1982, 2010
Sau96I	GGNCC	4 cut(s)	283, 589, 679, 694
SchI	GAGTC	1 cut(s)	141
ScrFI	CCNGG	5 cut(s)	281, 683, 691, 698, 862
SduI	GDGCHC	5 cut(s)	342, 423, 580, 1004, 1415
SexAI	ACCWGGT	1 cut(s)	696
SfaNI	GCATC	6 cut(s)	1144, 1185, 1786, 1803, 1963, 1980
SfcI	CTRYAG	1 cut(s)	662
SfoI	GGCGCC	2 cut(s)	431, 585
SfuI	TTCGAA	1 cut(s)	1189
SinI	GGWCC	4 cut(s)	283, 589, 679, 694
SmiMI	CAYNNNNRTG	1 cut(s)	1694
SmlI	CTYRAG	4 cut(s)	1106, 1265, 1511, 1663
SmoI	CTYRAG	4 cut(s)	1106, 1265, 1511, 1663
Sse9I	AATT	6 cut(s)	103, 756, 1373, 1545, 1684, 1845
SspDI	GGCGCC	2 cut(s)	429, 583
SspI	AATATT	1 cut(s)	1027
SspMI	CTAG	2 cut(s)	729, 1751
SstI	GAGCTC	2 cut(s)	580, 1415
StyD4I	CCNGG	5 cut(s)	279, 681, 689, 696, 860
StyI	CCWWGG	1 cut(s)	265
TaaI	ACNGT	3 cut(s)	738, 910, 1897
TaiI	ACGT	5 cut(s)	468, 504, 519, 1022, 1232
TasI	AATT	6 cut(s)	103, 756, 1373, 1545, 1684, 1845
TatI	WGTACW	2 cut(s)	5, 936
TauI	GCSGC	4 cut(s)	258, 435, 650, 1388
TfiI	GAWTC	2 cut(s)	1315, 2000
Tru1I	TTAA	3 cut(s)	1512, 1890, 1995
Tru9I	TTAA	3 cut(s)	1512, 1890, 1995
TscAI	CASTG	2 cut(s)	539, 913
TseFI	GTSAC	1 cut(s)	1225
TseI	GCWGC	2 cut(s)	661, 1048
Tsp45I	GTSAC	1 cut(s)	1225
TspGWI	ACGGA	2 cut(s)	475, 1586
TspRI	CASTG	2 cut(s)	539, 913
Tth111I	GACNNNGTC	1 cut(s)	220
Van91I	CCANNNNNTGG	1 cut(s)	534
Vha464I	CTTAAG	1 cut(s)	1511
VpaK11BI	GGWCC	4 cut(s)	283, 589, 679, 694
XapI	RAATTY	1 cut(s)	1845
XceI	RCATGY	1 cut(s)	640
XcmI	CCANNNNNNNNNTGG	1 cut(s)	394
XmiI	GTMKAC	1 cut(s)	294
XmnI	GAANNNNTTC	1 cut(s)	1176
XspI	CTAG	2 cut(s)	729, 1751
ZraI	GACGTC	1 cut(s)	466

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.