pycom11g00170

Glucosidase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0013050
Physical Location & Seq
Reverse (-)
153181 .. 156565
3385 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 912 bp
ATGATCGATAGTTATACTCCTGAACATAAAGGATCATTGGTTGAGAGTGTAGAACAGCATGAAGAAAAGCAGAAAGAAGAGCCTGTTGCTATATCTGAAAATGAATTTGATTCTGGAAGCAAGGTGCCTCCTGATGAGGGCGAGGATGAATCTGAAAAGACCGAGTCATTGTCCAGGGAAGAGTTAGGCCGCCGTGTTGCTTCTCGTTGGACTGGTGAAGATACCACAAAGGAAGGAGAAGAAGTTGATGCTGTAAAAGAGGACACTCATGAGGAGTATGATGGCTATGCTTCAGAAACTGATGATGACAACCAAAAATATGACGACGAAGATGTGGAGGAGGCGGCAGATAATGATTTTGGAGAGGAGGATCACGGCGATTCTGATTCTTCTTACAAATATGAGTCAGACACTGATTCAGACTTTTCAGATGTAACAACTTCAAGTACTCCATCTTGGTTGGAGAAGATACAAAAAACTGTGCGGAACCTTCTACAGGCTGTTAATTTATTCCAAACTCCAGTGAACCAATCAGAGGCTGCTAGTATACGCAAGGAGTATGACGAATCCAGTGCCAAGTATGTTTACAAAGTATGCCCATATAAACAAGCTTCCCAGGTGGAGGGCCACTCTACAACCCGTTTAGGGAGCTGGGACAAATTTGAGGACTCGTACAAAGTCATGGTCTTTGCAAATGGTGATAAGTGCTGGAATGGGCCTGATAGAAGTTTGAAGGTCAGACTTAGATGTGGATTGAAAAATGAGGTTGCTGATGTTGATGAACCAAGTCGTTGCGAGTACGTAGCGTTGTTTTCTACACCAGCGCTTTGCTTAGAGGAAAAACTTAAGGAACTGCAACACAAATTAGTCGTATTGAACAAAGAAGAACCACAAGGCCACGATGAACTTTAA

Protein Analysis

304

Amino Acids

34.4

Weight (kDa)

4.38

Isoelectric Point (pI)

53.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRKCSH_1 PF13015 187 - 285 3.7e-20 Glucosidase II beta subunit-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013050)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56360
fragaria_vesca FvH4_3g45911
malus_domestica MD03G1001800.v1.1 MD11G1001700.v1.1
prunus_persica Prupe.6G001700_v2.0.a1 Prupe.6G001700_v2.0.a1
pyrus_communis pycom03g00180 pycom11g00170
rosa_chinensis RchiOBHm_Chr5g0084021
rosa_laevigata RLG00000037073
rosa_multiflora Rmu_ssc0000372.1_g000082
rosa_roxburghii Rroxscaffold_1G00000640
rosa_rugosa Rorug05G0488400
rosa_samantha Rh5AG541000 Rh5BG566300 Rh5CG588300 Rh5DG574600
rosa_wichuraiana Rw5G050310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 124
AccI GTMKAC 1 cut(s) 547
AciI CCGC 3 cut(s) 190, 344, 484
AclWI GGATC 2 cut(s) 40, 378
AcsI RAATTY 2 cut(s) 104, 659
AcuI CTGAAG 1 cut(s) 276
AfaI GTAC 3 cut(s) 448, 674, 800
AfeI AGCGCT 1 cut(s) 825
AfiI CCNNNNNNNGG 5 cut(s) 137, 496, 535, 622, 645
AflII CTTAAG 1 cut(s) 845
AgsI TTSAA 4 cut(s) 444, 733, 757, 877
AjnI CCWGG 2 cut(s) 173, 615
AluBI AGCT 2 cut(s) 611, 651
AluI AGCT 2 cut(s) 611, 651
AlwI GGATC 2 cut(s) 40, 378
AlwNI CAGNNNCTG 3 cut(s) 299, 413, 539
Aor51HI AGCGCT 1 cut(s) 825
AoxI GGCC 4 cut(s) 187, 625, 716, 895
ApeKI GCWGC 1 cut(s) 539
ApoI RAATTY 2 cut(s) 104, 659
ArsI GACNNNNNNTTYG 2 cut(s) 669, 701
AspLEI GCGC 1 cut(s) 826
AspS9I GGNCC 2 cut(s) 625, 716
AsuHPI GGTGA 2 cut(s) 227, 710
BanI GGYRCC 1 cut(s) 124
BbvI GCAGC 1 cut(s) 526
BccI CCATC 2 cut(s) 275, 460
BceAI ACGGC 2 cut(s) 177, 391
BcgI CGANNNNNNTGC 2 cut(s) 554, 588
BciT130I CCWGG 2 cut(s) 175, 617
BfaI CTAG 1 cut(s) 543
BfmI CTRYAG 1 cut(s) 494
BfoI RGCGCY 1 cut(s) 827
BfrI CTTAAG 1 cut(s) 845
BisI GCNGC 3 cut(s) 190, 345, 540
BlsI GCNGC 3 cut(s) 191, 346, 541
BmcAI AGTACT 1 cut(s) 448
Bme1390I CCNGG 2 cut(s) 175, 617
BmgT120I GGNCC 2 cut(s) 625, 716
BmiI GGNNCC 2 cut(s) 126, 488
BmrFI CCNGG 2 cut(s) 175, 617
BmsI GCATC 1 cut(s) 238
BplI GAGNNNNNCTC 2 cut(s) 614, 646
BpmI CTGGAG 1 cut(s) 504
Bsa29I ATCGAT 1 cut(s) 6
BsaAI YACGTR 1 cut(s) 802
BsaJI CCNNGG 2 cut(s) 174, 615
BsaXI ACNNNNNCTCC 2 cut(s) 228, 258
Bsc4I CCNNNNNNNGG 5 cut(s) 137, 496, 535, 622, 645
Bse1I ACTGG 3 cut(s) 217, 521, 570
BseBI CCWGG 2 cut(s) 175, 617
BseCI ATCGAT 1 cut(s) 6
BseDI CCNNGG 2 cut(s) 174, 615
BseGI GGATG 1 cut(s) 151
BseLI CCNNNNNNNGG 5 cut(s) 137, 496, 535, 622, 645
BseNI ACTGG 3 cut(s) 217, 521, 570
BseRI GAGGAG 3 cut(s) 287, 353, 380
BseXI GCAGC 1 cut(s) 526
BseYI CCCAGC 1 cut(s) 651
BshFI GGCC 4 cut(s) 189, 627, 718, 897
BshNI GGYRCC 1 cut(s) 124
BshVI ATCGAT 1 cut(s) 6
BslFI GGGAC 1 cut(s) 668
BslI CCNNNNNNNGG 5 cut(s) 137, 496, 535, 622, 645
BsmFI GGGAC 1 cut(s) 668
BsnI GGCC 4 cut(s) 189, 627, 718, 897
Bsp143I GATC 3 cut(s) 3, 32, 370
BspACI CCGC 3 cut(s) 190, 344, 484
BspANI GGCC 4 cut(s) 189, 627, 718, 897
BspDI ATCGAT 1 cut(s) 6
BspHI TCATGA 1 cut(s) 268
BspLI GGNNCC 2 cut(s) 126, 488
BspPI GGATC 2 cut(s) 40, 378
BspQI GCTCTTC 1 cut(s) 72
BspT107I GGYRCC 1 cut(s) 124
BspTI CTTAAG 1 cut(s) 845
BsrI ACTGG 3 cut(s) 217, 521, 570
BssECI CCNNGG 2 cut(s) 174, 615
BssMI GATC 3 cut(s) 3, 32, 370
BssNAI GTATAC 1 cut(s) 548
Bst1107I GTATAC 1 cut(s) 548
Bst2UI CCWGG 2 cut(s) 175, 617
Bst4CI ACNGT 1 cut(s) 481
Bst6I CTCTTC 2 cut(s) 72, 174
BstAFI CTTAAG 1 cut(s) 845
BstBAI YACGTR 1 cut(s) 802
BstDEI CTNAG 2 cut(s) 743, 832
BstENI CCTNNNNNAGG 1 cut(s) 494
BstF5I GGATG 1 cut(s) 151
BstH2I RGCGCY 1 cut(s) 827
BstHHI GCGC 1 cut(s) 826
BstKTI GATC 3 cut(s) 6, 35, 373
BstMBI GATC 3 cut(s) 3, 32, 370
BstNI CCWGG 2 cut(s) 175, 617
BstSCI CCNGG 2 cut(s) 173, 615
BstSFI CTRYAG 1 cut(s) 494
BstSNI TACGTA 1 cut(s) 802
BstV1I GCAGC 1 cut(s) 526
BstZ17I GTATAC 1 cut(s) 548
Bsu15I ATCGAT 1 cut(s) 6
BsuRI GGCC 4 cut(s) 189, 627, 718, 897
BsuTUI ATCGAT 1 cut(s) 6
BtsCI GGATG 1 cut(s) 151
BtsIMutI CAGTG 3 cut(s) 411, 528, 577
CaiI CAGNNNCTG 3 cut(s) 299, 413, 539
CciI TCATGA 1 cut(s) 268
CfoI GCGC 1 cut(s) 826
Cfr13I GGNCC 2 cut(s) 625, 716
ClaI ATCGAT 1 cut(s) 6
Csp6I GTAC 3 cut(s) 447, 673, 799
CviAII CATG 3 cut(s) 59, 269, 682
CviQI GTAC 3 cut(s) 447, 673, 799
DdeI CTNAG 2 cut(s) 743, 832
DpnI GATC 3 cut(s) 5, 34, 372
DpnII GATC 3 cut(s) 3, 32, 370
Eam1104I CTCTTC 2 cut(s) 72, 174
EarI CTCTTC 2 cut(s) 72, 174
Eco105I TACGTA 1 cut(s) 802
Eco47III AGCGCT 1 cut(s) 825
Eco57I CTGAAG 1 cut(s) 276
EcoNI CCTNNNNNAGG 1 cut(s) 494
EcoRII CCWGG 2 cut(s) 173, 615
FaeI CATG 3 cut(s) 62, 272, 685
FaqI GGGAC 1 cut(s) 668
FatI CATG 3 cut(s) 58, 268, 681
FblI GTMKAC 1 cut(s) 547
Fnu4HI GCNGC 3 cut(s) 190, 345, 540
FokI GGATG 1 cut(s) 158
Fsp4HI GCNGC 3 cut(s) 190, 345, 540
FspBI CTAG 1 cut(s) 543
GlaI GCGC 1 cut(s) 825
GluI GCNGC 3 cut(s) 190, 345, 540
GsaI CCCAGC 1 cut(s) 655
GsuI CTGGAG 1 cut(s) 504
HaeII RGCGCY 1 cut(s) 827
HaeIII GGCC 4 cut(s) 189, 627, 718, 897
HhaI GCGC 1 cut(s) 826
Hin1II CATG 3 cut(s) 62, 272, 685
Hin6I GCGC 1 cut(s) 824
HinP1I GCGC 1 cut(s) 824
HindIII AAGCTT 1 cut(s) 609
HinfI GANTC 9 cut(s) 110, 149, 164, 380, 386, 404, 416, 566, 668
HphI GGTGA 2 cut(s) 227, 710
Hpy166II GTNNAC 3 cut(s) 526, 548, 586
Hpy188I TCNGA 9 cut(s) 97, 154, 295, 385, 409, 421, 430, 535, 740
Hpy188III TCNNGA 4 cut(s) 20, 114, 131, 269
Hpy8I GTNNAC 3 cut(s) 526, 548, 586
Hpy99I CGWCG 1 cut(s) 329
HpyAV CCTTC 3 cut(s) 227, 500, 727
HpyCH4III ACNGT 1 cut(s) 481
HpyCH4IV ACGT 1 cut(s) 801
HpyCH4V TGCA 2 cut(s) 692, 856
HpyF3I CTNAG 2 cut(s) 743, 832
HpySE526I ACGT 1 cut(s) 801
Hsp92II CATG 3 cut(s) 62, 272, 685
HspAI GCGC 1 cut(s) 824
Kzo9I GATC 3 cut(s) 3, 32, 370
LguI GCTCTTC 1 cut(s) 72
LmnI GCTCC 1 cut(s) 648
Lsp1109I GCAGC 1 cut(s) 526
LweI GCATC 1 cut(s) 238
MaeI CTAG 1 cut(s) 543
MaeII ACGT 1 cut(s) 801
MaeIII GTNAC 1 cut(s) 433
MalI GATC 3 cut(s) 5, 34, 372
MboI GATC 3 cut(s) 3, 32, 370
MboII GAAGA 9 cut(s) 74, 89, 191, 230, 251, 341, 381, 478, 896
MluCI AATT 4 cut(s) 104, 505, 659, 863
MlyI GAGTC 3 cut(s) 173, 413, 662
MmeI TCCRAC 2 cut(s) 188, 441
MseI TTAA 3 cut(s) 504, 846, 910
MspCI CTTAAG 1 cut(s) 845
MspR9I CCNGG 2 cut(s) 175, 617
MvaI CCWGG 2 cut(s) 175, 617
NdeII GATC 3 cut(s) 3, 32, 370
NlaIII CATG 3 cut(s) 62, 272, 685
NlaIV GGNNCC 2 cut(s) 126, 488
PagI TCATGA 1 cut(s) 268
PciSI GCTCTTC 1 cut(s) 72
PfeI GAWTC 6 cut(s) 110, 149, 380, 386, 416, 566
PflFI GACNNNGTC 1 cut(s) 163
PkrI GCNGC 3 cut(s) 191, 346, 541
PleI GAGTC 3 cut(s) 172, 412, 662
PpsI GAGTC 3 cut(s) 172, 412, 662
Ppu21I YACGTR 1 cut(s) 802
Psp6I CCWGG 2 cut(s) 173, 615
PspFI CCCAGC 1 cut(s) 651
PspGI CCWGG 2 cut(s) 173, 615
PspN4I GGNNCC 2 cut(s) 126, 488
PspPI GGNCC 2 cut(s) 625, 716
PstNI CAGNNNCTG 3 cut(s) 299, 413, 539
PsyI GACNNNGTC 1 cut(s) 163
RsaI GTAC 3 cut(s) 448, 674, 800
RsaNI GTAC 3 cut(s) 447, 673, 799
SapI GCTCTTC 1 cut(s) 72
SaqAI TTAA 3 cut(s) 504, 846, 910
SatI GCNGC 3 cut(s) 190, 345, 540
Sau3AI GATC 3 cut(s) 3, 32, 370
Sau96I GGNCC 2 cut(s) 625, 716
ScaI AGTACT 1 cut(s) 448
SchI GAGTC 3 cut(s) 173, 413, 662
ScrFI CCNGG 2 cut(s) 175, 617
SetI ASST 8 cut(s) 126, 492, 613, 621, 653, 738, 768, 804
SfaNI GCATC 1 cut(s) 238
SfcI CTRYAG 1 cut(s) 494
SmlI CTYRAG 1 cut(s) 845
SmoI CTYRAG 1 cut(s) 845
SnaBI TACGTA 1 cut(s) 802
Sse9I AATT 4 cut(s) 104, 505, 659, 863
SsiI CCGC 3 cut(s) 190, 344, 484
SspMI CTAG 1 cut(s) 543
StyD4I CCNGG 2 cut(s) 173, 615
TaaI ACNGT 1 cut(s) 481
TaiI ACGT 1 cut(s) 804
TaqI TCGA 1 cut(s) 6
TaqII GACCGA 1 cut(s) 176
TasI AATT 4 cut(s) 104, 505, 659, 863
TatI WGTACW 1 cut(s) 446
TauI GCSGC 2 cut(s) 192, 347
TfiI GAWTC 6 cut(s) 110, 149, 380, 386, 416, 566
Tru1I TTAA 3 cut(s) 504, 846, 910
Tru9I TTAA 3 cut(s) 504, 846, 910
TscAI CASTG 3 cut(s) 418, 528, 577
TseI GCWGC 1 cut(s) 539
TspDTI ATGAA 4 cut(s) 75, 117, 162, 795
TspRI CASTG 3 cut(s) 418, 528, 577
Tth111I GACNNNGTC 1 cut(s) 163
Vha464I CTTAAG 1 cut(s) 845
XagI CCTNNNNNAGG 1 cut(s) 494
XapI RAATTY 2 cut(s) 104, 659
XmiI GTMKAC 1 cut(s) 547
XspI CTAG 1 cut(s) 543
ZrmI AGTACT 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.