pycom11g08040

Serine arginine repetitive matrix protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007711
Physical Location & Seq
Reverse (-)
6171613 .. 6174986
3374 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1701 bp
ATGTCTGGCGGGTTTTTCAGGGGTACGTCCGCTGATCAGGACACTAGGTTTTCCAATAAGCAAGCGAAGCTGATGAAATCTCAGAAATTTGCACCTGAATTGGAACATTTGGTCGATATAACGAAGGTCAGTATGGATGTTATGCGCCCGTGGATTGCTAATCGAGTAACGGAGCTTCTCGGATTCGAAGATGAAGTTCTTATCAACTTTATCTATGGTCTTTTGGAAGGAAAGGTGGTGAATGGGAAGGAAATTCAAATCTCGCTTACTGGATTTATGGAGAAAAACACTGTGAAGTTTATGAAAGAGCTTTGGACACTGCTTATCAGTGCACAGAACAATGCTAGTGGTGTTCCTCAACAGTTTTTAGATGCCAAACAAGAAGAAGCACGGAAAAAGAAGGAGGAGGCAGACAAGTTTGCAAGTGACATTCAGAGAAAGAAAGAGAAGGAGAGGATAGATCAACAAGAGATATTGAAGAAGATGGAAGAAGGGGCTGACAAGAAAGCAAACAGTGCTGCATTGGAGCCAAGCACAAAGCGTATGTCGCCAAGGGATTCAAATGATCATTATGAGGATGAAAGAGGAGCTGAAAAGAGGATTGGTGTGAAGGCAAGGAGCAAGGTTTCCAGATCTCCAAATTCTGAAAATTCATCTTTGTCTCCTCGTGAAAGACACCGATCAAGAAGTATTTCCAAGTCACCAAAAGCGTTGAGACGATCCATTTCTTCTGATAGGAGTGATCGCTCTTCACCAAGACGGCCTATTACAACTGTTCGGAGGCATTCGCCAGAGGGATCAACCTCCCCAAGAGGAAGATCACGTTATTCCAGGCAAAGATCTAGATCAAATTTCCAACAAAGATCACCCTCTCCGTATCGGCGTAGAGTACATTCTCCATATTGGCGTAGACCCTCACCTGTCAGGCGCCGCAGATCACCATCCCCAGTCCGTCGCCGCAGATCACCATCGCCAGTCCGGCGCCGCAGATCACCTTCGCCAGTCCGGCGCCACAGATCACCTTCACCAGTCCGGCGCCGCAGATCACCTTCACCAGTCCGGCGCCGCAGATCACCCTCGCCTATTCGACGTCCCAGATCACCCTCGCCTGTGCGACGTCCCAGATCACCCTCGCCTGTGCGACGTCCCAGATCACCCTCGCCTGTGCGGCGTCGCAGGTCACCCTCTCCTTTGCGACGTCGCAGGTCGCCCTCTCCTTTGCGACGTCGCAGATCTCGCTCACCTGTGCGTCGCAGATCACCCTCACCTGTACGACGGGCGTATTGTAGATCCCCATTGAATACACGGAACAGGTATGTGTCTCCCTTGCAACATAGGTCACCAGTTCGTAGCAGGAGGTCACCAACTCCTCCGCATAGGTCTCCGTCTCCGCATGATCGAAGTTCATCTCCTATTCAGCATGGTTCCCCTTCGCCAATCAGGAGAACATCAAAGCTTCAGAGATCTCCCTCACAATCTCCTCAAGAAAGAACCAGGTCCCGTGAAAAAATTTCTCCTGTGCCACACCGGTCTAGCTCGTATGGGTCATTGCAGAGGGAATTAAAGAATGGGAATGATTCGCGTAAAAGAGCACCAGATTTGTCGCCATCACCAGTGAGGTCTCGTTTACTCTCAGAATCTCCACCAGGCGTAAGAAGTGATAGTGAAGAAAGGAGGTATTCATCTTGGCTGCATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
PWI

Protein Analysis

567

Amino Acids

65.69

Weight (kDa)

11.91

Isoelectric Point (pI)

153.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWI PF01480 46 - 115 5.1e-28 PWI domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 5 cut(s) 1093, 1120, 1147, 1201, 1228
Acc36I ACCTGC 2 cut(s) 1167, 1194
AccB1I GGYRCC 5 cut(s) 927, 981, 1008, 1035, 1062
AccI GTMKAC 1 cut(s) 910
AccII CGCG 1 cut(s) 1582
AclWI GGATC 3 cut(s) 714, 805, 1284
AcsI RAATTY 6 cut(s) 86, 252, 640, 649, 850, 1509
AcuI CTGAAG 1 cut(s) 1442
AfaI GTAC 3 cut(s) 25, 891, 1272
AgeI ACCGGT 1 cut(s) 1527
AgsI TTSAA 4 cut(s) 257, 478, 561, 1300
AjnI CCWGG 3 cut(s) 830, 1493, 1645
AjuI GAANNNNNNNTTGG 2 cut(s) 585, 617
AluBI AGCT 6 cut(s) 70, 175, 310, 590, 1456, 1536
AluI AGCT 6 cut(s) 70, 175, 310, 590, 1456, 1536
Alw21I GWGCWC 2 cut(s) 334, 1594
Alw26I GTCTC 6 cut(s) 666, 709, 1326, 1386, 1392, 1626
Alw44I GTGCAC 1 cut(s) 330
AlwI GGATC 3 cut(s) 714, 805, 1284
AoxI GGCC 1 cut(s) 761
ApaLI GTGCAC 1 cut(s) 330
ApeKI GCWGC 2 cut(s) 518, 1690
ApoI RAATTY 6 cut(s) 86, 252, 640, 649, 850, 1509
ArsI GACNNNNNNTTYG 2 cut(s) 530, 562
AsiGI ACCGGT 1 cut(s) 1527
Asp700I GAANNNNTTC 1 cut(s) 691
AspLEI GCGC 6 cut(s) 147, 930, 984, 1011, 1038, 1065
AspS9I GGNCC 1 cut(s) 1497
AsuII TTCGAA 1 cut(s) 186
AvaII GGWCC 1 cut(s) 1497
BaeGI GKGCMC 1 cut(s) 334
BanI GGYRCC 5 cut(s) 927, 981, 1008, 1035, 1062
BarI GAAGNNNNNNTAC 2 cut(s) 159, 191
BauI CACGAG 1 cut(s) 666
Bbv12I GWGCWC 2 cut(s) 334, 1594
BbvI GCAGC 2 cut(s) 505, 1677
BccI CCATC 4 cut(s) 478, 949, 976, 1615
BceAI ACGGC 1 cut(s) 776
BciT130I CCWGG 3 cut(s) 832, 1495, 1647
BclI TGATCA 2 cut(s) 34, 565
BcoDI GTCTC 6 cut(s) 666, 709, 1326, 1386, 1392, 1626
BfaI CTAG 4 cut(s) 45, 345, 843, 1533
BfoI RGCGCY 5 cut(s) 931, 985, 1012, 1039, 1066
BfuAI ACCTGC 2 cut(s) 1167, 1194
BglI GCCNNNNNGGC 3 cut(s) 979, 1006, 1168
BglII AGATCT 4 cut(s) 632, 839, 1232, 1463
BisI GCNGC 8 cut(s) 519, 931, 958, 985, 1039, 1066, 1169, 1691
BlsI GCNGC 8 cut(s) 520, 932, 959, 986, 1040, 1067, 1170, 1692
Bme1390I CCNGG 3 cut(s) 832, 1495, 1647
Bme18I GGWCC 1 cut(s) 1497
BmgT120I GGNCC 1 cut(s) 1497
BmiI GGNNCC 8 cut(s) 528, 929, 983, 1010, 1037, 1064, 1426, 1499
BmrFI CCNGG 3 cut(s) 832, 1495, 1647
BmrI ACTGGG 1 cut(s) 941
BmsI GCATC 1 cut(s) 361
BmuI ACTGGG 1 cut(s) 941
Bpu14I TTCGAA 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 1467
BsaBI GATNNNNATC 3 cut(s) 844, 940, 967
BsaI GGTCTC 2 cut(s) 1386, 1626
BsaJI CCNNGG 2 cut(s) 149, 551
BsaWI WCCGGW 1 cut(s) 1527
Bse118I RCCGGY 1 cut(s) 1527
Bse1I ACTGG 8 cut(s) 274, 947, 974, 1001, 1028, 1055, 1343, 1613
Bse3DI GCAATG 1 cut(s) 1547
Bse8I GATNNNNATC 3 cut(s) 844, 940, 967
BseBI CCWGG 3 cut(s) 832, 1495, 1647
BseDI CCNNGG 2 cut(s) 149, 551
BseGI GGATG 3 cut(s) 142, 583, 941
BseJI GATNNNNATC 3 cut(s) 844, 940, 967
BseMI GCAATG 1 cut(s) 1547
BseMII CTCAG 2 cut(s) 95, 1647
BseNI ACTGG 8 cut(s) 274, 947, 974, 1001, 1028, 1055, 1343, 1613
BseRI GAGGAG 5 cut(s) 419, 600, 654, 1359, 1470
BseSI GKGCMC 1 cut(s) 334
BseXI GCAGC 2 cut(s) 505, 1677
Bsh1236I CGCG 1 cut(s) 1582
BshFI GGCC 1 cut(s) 763
BshNI GGYRCC 5 cut(s) 927, 981, 1008, 1035, 1062
BshTI ACCGGT 1 cut(s) 1527
BsiHKAI GWGCWC 2 cut(s) 334, 1594
BsiSI CCGG 5 cut(s) 979, 1006, 1033, 1060, 1528
BslFI GGGAC 4 cut(s) 1077, 1104, 1131, 1483
BsmAI GTCTC 6 cut(s) 666, 709, 1326, 1386, 1392, 1626
BsmBI CGTCTC 2 cut(s) 709, 1392
BsmFI GGGAC 4 cut(s) 1077, 1104, 1131, 1483
BsmI GAATGC 1 cut(s) 784
BsnI GGCC 1 cut(s) 763
Bso31I GGTCTC 2 cut(s) 1386, 1626
Bsp119I TTCGAA 1 cut(s) 186
Bsp1286I GDGCHC 2 cut(s) 334, 1594
BspANI GGCC 1 cut(s) 763
BspCNI CTCAG 2 cut(s) 94, 1646
BspFNI CGCG 1 cut(s) 1582
BspLI GGNNCC 8 cut(s) 528, 929, 983, 1010, 1037, 1064, 1426, 1499
BspMI ACCTGC 2 cut(s) 1167, 1194
BspPI GGATC 3 cut(s) 714, 805, 1284
BspQI GCTCTTC 1 cut(s) 754
BspT104I TTCGAA 1 cut(s) 186
BspT107I GGYRCC 5 cut(s) 927, 981, 1008, 1035, 1062
BspTNI GGTCTC 2 cut(s) 1386, 1626
BsrDI GCAATG 1 cut(s) 1547
BsrFI RCCGGY 1 cut(s) 1527
BsrI ACTGG 8 cut(s) 274, 947, 974, 1001, 1028, 1055, 1343, 1613
BssAI RCCGGY 1 cut(s) 1527
BssECI CCNNGG 2 cut(s) 149, 551
BssSI CACGAG 1 cut(s) 666
BssT1I CCWWGG 1 cut(s) 551
Bst2BI CACGAG 1 cut(s) 666
Bst2UI CCWGG 3 cut(s) 832, 1495, 1647
Bst4CI ACNGT 4 cut(s) 292, 363, 515, 775
Bst6I CTCTTC 1 cut(s) 754
BstAPI GCANNNNNTGC 1 cut(s) 515
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 63
BstDEI CTNAG 2 cut(s) 81, 1633
BstDSI CCRYGG 1 cut(s) 149
BstEII GGTNACC 3 cut(s) 1179, 1338, 1359
BstF5I GGATG 3 cut(s) 142, 583, 941
BstFNI CGCG 1 cut(s) 1582
BstH2I RGCGCY 5 cut(s) 931, 985, 1012, 1039, 1066
BstHHI GCGC 6 cut(s) 147, 930, 984, 1011, 1038, 1065
BstMAI GTCTC 6 cut(s) 666, 709, 1326, 1386, 1392, 1626
BstMWI GCNNNNNNNGC 7 cut(s) 67, 515, 547, 979, 1006, 1168, 1236
BstNI CCWGG 3 cut(s) 832, 1495, 1647
BstPI GGTNACC 3 cut(s) 1179, 1338, 1359
BstSCI CCNGG 3 cut(s) 830, 1493, 1645
BstSLI GKGCMC 1 cut(s) 334
BstUI CGCG 1 cut(s) 1582
BstV1I GCAGC 2 cut(s) 505, 1677
BstX2I RGATCY 5 cut(s) 632, 839, 1232, 1289, 1463
BstYI RGATCY 5 cut(s) 632, 839, 1232, 1289, 1463
BsuRI GGCC 1 cut(s) 763
BtgI CCRYGG 1 cut(s) 149
BtgZI GCGATG 1 cut(s) 954
BtsCI GGATG 3 cut(s) 142, 583, 941
BtsI GCAGTG 1 cut(s) 317
BtsIMutI CAGTG 5 cut(s) 288, 317, 334, 520, 1620
BveI ACCTGC 2 cut(s) 1167, 1194
Cac8I GCNNGC 1 cut(s) 63
CfoI GCGC 6 cut(s) 147, 930, 984, 1011, 1038, 1065
Cfr10I RCCGGY 1 cut(s) 1527
Cfr13I GGNCC 1 cut(s) 1497
CseI GACGC 2 cut(s) 1160, 1238
CsiI ACCWGGT 1 cut(s) 1493
Csp6I GTAC 3 cut(s) 24, 890, 1271
CspAI ACCGGT 1 cut(s) 1527
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviAII CATG 3 cut(s) 1394, 1421, 1694
CviQI GTAC 3 cut(s) 24, 890, 1271
DdeI CTNAG 2 cut(s) 81, 1633
DinI GGCGCC 5 cut(s) 929, 983, 1010, 1037, 1064
Eam1104I CTCTTC 1 cut(s) 754
EarI CTCTTC 1 cut(s) 754
Eco130I CCWWGG 1 cut(s) 551
Eco31I GGTCTC 2 cut(s) 1386, 1626
Eco47I GGWCC 1 cut(s) 1497
Eco57I CTGAAG 1 cut(s) 1442
Eco91I GGTNACC 3 cut(s) 1179, 1338, 1359
EcoO109I RGGNCCY 1 cut(s) 1497
EcoO65I GGTNACC 3 cut(s) 1179, 1338, 1359
EcoRII CCWGG 3 cut(s) 830, 1493, 1645
EcoT14I CCWWGG 1 cut(s) 551
EgeI GGCGCC 5 cut(s) 929, 983, 1010, 1037, 1064
EheI GGCGCC 5 cut(s) 929, 983, 1010, 1037, 1064
ErhI CCWWGG 1 cut(s) 551
Esp3I CGTCTC 2 cut(s) 709, 1392
FaeI CATG 3 cut(s) 1397, 1424, 1697
FaqI GGGAC 4 cut(s) 1077, 1104, 1131, 1483
FatI CATG 3 cut(s) 1393, 1420, 1693
FauI CCCGC 1 cut(s) 2
FbaI TGATCA 2 cut(s) 34, 565
FblI GTMKAC 1 cut(s) 910
Fnu4HI GCNGC 8 cut(s) 519, 931, 958, 985, 1039, 1066, 1169, 1691
FokI GGATG 3 cut(s) 149, 590, 928
Fsp4HI GCNGC 8 cut(s) 519, 931, 958, 985, 1039, 1066, 1169, 1691
FspBI CTAG 4 cut(s) 45, 345, 843, 1533
GlaI GCGC 6 cut(s) 146, 929, 983, 1010, 1037, 1064
GluI GCNGC 8 cut(s) 519, 931, 958, 985, 1039, 1066, 1169, 1691
HaeII RGCGCY 5 cut(s) 931, 985, 1012, 1039, 1066
HaeIII GGCC 1 cut(s) 763
HapII CCGG 5 cut(s) 979, 1006, 1033, 1060, 1528
HgaI GACGC 2 cut(s) 1160, 1238
HhaI GCGC 6 cut(s) 147, 930, 984, 1011, 1038, 1065
Hin1II CATG 3 cut(s) 1397, 1424, 1697
Hin6I GCGC 6 cut(s) 145, 928, 982, 1009, 1036, 1063
HinP1I GCGC 6 cut(s) 145, 928, 982, 1009, 1036, 1063
HindIII AAGCTT 1 cut(s) 1454
HinfI GANTC 4 cut(s) 183, 557, 1577, 1637
HpaII CCGG 5 cut(s) 979, 1006, 1033, 1060, 1528
Hpy166II GTNNAC 3 cut(s) 332, 911, 1628
Hpy188I TCNGA 8 cut(s) 84, 182, 435, 646, 733, 780, 1461, 1636
Hpy188III TCNNGA 7 cut(s) 38, 630, 668, 684, 843, 1441, 1484
Hpy8I GTNNAC 3 cut(s) 332, 911, 1628
HpyCH4III ACNGT 4 cut(s) 292, 363, 515, 775
HpyCH4IV ACGT 7 cut(s) 26, 823, 1090, 1117, 1144, 1198, 1225
HpyCH4V TGCA 7 cut(s) 92, 332, 422, 521, 1330, 1552, 1693
HpyF10VI GCNNNNNNNGC 7 cut(s) 67, 515, 547, 979, 1006, 1168, 1236
HpyF3I CTNAG 2 cut(s) 81, 1633
HpySE526I ACGT 7 cut(s) 26, 823, 1090, 1117, 1144, 1198, 1225
Hsp92II CATG 3 cut(s) 1397, 1424, 1697
HspAI GCGC 6 cut(s) 145, 928, 982, 1009, 1036, 1063
KasI GGCGCC 5 cut(s) 927, 981, 1008, 1035, 1062
Ksp22I TGATCA 2 cut(s) 34, 565
LguI GCTCTTC 1 cut(s) 754
LmnI GCTCC 4 cut(s) 172, 526, 587, 618
Lsp1109I GCAGC 2 cut(s) 505, 1677
LweI GCATC 1 cut(s) 361
MabI ACCWGGT 1 cut(s) 1493
MaeI CTAG 4 cut(s) 45, 345, 843, 1533
MaeII ACGT 7 cut(s) 26, 823, 1090, 1117, 1144, 1198, 1225
MaeIII GTNAC 6 cut(s) 166, 425, 699, 1179, 1338, 1359
MboII GAAGA 9 cut(s) 200, 395, 490, 493, 500, 720, 741, 828, 1679
MflI RGATCY 5 cut(s) 632, 839, 1232, 1289, 1463
MhlI GDGCHC 2 cut(s) 334, 1594
MluCI AATT 8 cut(s) 86, 98, 252, 640, 649, 850, 1509, 1559
Mly113I GGCGCC 5 cut(s) 928, 982, 1009, 1036, 1063
MmeI TCCRAC 1 cut(s) 880
MroXI GAANNNNTTC 1 cut(s) 691
MseI TTAA 1 cut(s) 1562
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 5 cut(s) 979, 1006, 1033, 1060, 1528
MspR9I CCNGG 3 cut(s) 832, 1495, 1647
Mva1269I GAATGC 1 cut(s) 784
MvaI CCWGG 3 cut(s) 832, 1495, 1647
MvnI CGCG 1 cut(s) 1582
MwoI GCNNNNNNNGC 7 cut(s) 67, 515, 547, 979, 1006, 1168, 1236
NarI GGCGCC 5 cut(s) 928, 982, 1009, 1036, 1063
NlaIII CATG 3 cut(s) 1397, 1424, 1697
NlaIV GGNNCC 8 cut(s) 528, 929, 983, 1010, 1037, 1064, 1426, 1499
NmuCI GTSAC 5 cut(s) 425, 699, 1179, 1338, 1359
NspV TTCGAA 1 cut(s) 186
PciSI GCTCTTC 1 cut(s) 754
PctI GAATGC 1 cut(s) 784
PdmI GAANNNNTTC 1 cut(s) 691
PfeI GAWTC 4 cut(s) 183, 557, 1577, 1637
PinAI ACCGGT 1 cut(s) 1527
PkrI GCNGC 8 cut(s) 520, 932, 959, 986, 1040, 1067, 1170, 1692
PluTI GGCGCC 5 cut(s) 931, 985, 1012, 1039, 1066
PpuMI RGGWCCY 1 cut(s) 1497
Psp5II RGGWCCY 1 cut(s) 1497
Psp6I CCWGG 3 cut(s) 830, 1493, 1645
PspEI GGTNACC 3 cut(s) 1179, 1338, 1359
PspGI CCWGG 3 cut(s) 830, 1493, 1645
PspN4I GGNNCC 8 cut(s) 528, 929, 983, 1010, 1037, 1064, 1426, 1499
PspPI GGNCC 1 cut(s) 1497
PspPPI RGGWCCY 1 cut(s) 1497
PsuI RGATCY 5 cut(s) 632, 839, 1232, 1289, 1463
RsaI GTAC 3 cut(s) 25, 891, 1272
RsaNI GTAC 3 cut(s) 24, 890, 1271
SapI GCTCTTC 1 cut(s) 754
SaqAI TTAA 1 cut(s) 1562
SatI GCNGC 8 cut(s) 519, 931, 958, 985, 1039, 1066, 1169, 1691
Sau96I GGNCC 1 cut(s) 1497
ScrFI CCNGG 3 cut(s) 832, 1495, 1647
SduI GDGCHC 2 cut(s) 334, 1594
SexAI ACCWGGT 1 cut(s) 1493
SfaNI GCATC 1 cut(s) 361
SfoI GGCGCC 5 cut(s) 929, 983, 1010, 1037, 1064
SfuI TTCGAA 1 cut(s) 186
SinI GGWCC 1 cut(s) 1497
SmlI CTYRAG 1 cut(s) 1482
SmoI CTYRAG 1 cut(s) 1482
Sse9I AATT 8 cut(s) 86, 98, 252, 640, 649, 850, 1509, 1559
SspDI GGCGCC 5 cut(s) 927, 981, 1008, 1035, 1062
SspMI CTAG 4 cut(s) 45, 345, 843, 1533
StyD4I CCNGG 3 cut(s) 830, 1493, 1645
StyI CCWWGG 1 cut(s) 551
TaaI ACNGT 4 cut(s) 292, 363, 515, 775
TaiI ACGT 7 cut(s) 29, 826, 1093, 1120, 1147, 1201, 1228
TaqI TCGA 5 cut(s) 114, 163, 186, 1087, 1399
TasI AATT 8 cut(s) 86, 98, 252, 640, 649, 850, 1509, 1559
TatI WGTACW 1 cut(s) 889
TauI GCSGC 6 cut(s) 933, 960, 987, 1041, 1068, 1171
TfiI GAWTC 4 cut(s) 183, 557, 1577, 1637
Tru1I TTAA 1 cut(s) 1562
Tru9I TTAA 1 cut(s) 1562
TscAI CASTG 5 cut(s) 295, 324, 334, 520, 1620
TseFI GTSAC 5 cut(s) 425, 699, 1179, 1338, 1359
TseI GCWGC 2 cut(s) 518, 1690
Tsp45I GTSAC 5 cut(s) 425, 699, 1179, 1338, 1359
TspDTI ATGAA 7 cut(s) 89, 207, 317, 594, 642, 1395, 1671
TspGWI ACGGA 6 cut(s) 185, 406, 864, 941, 1321, 1374
TspRI CASTG 5 cut(s) 295, 324, 334, 520, 1620
VneI GTGCAC 1 cut(s) 330
VpaK11BI GGWCC 1 cut(s) 1497
XapI RAATTY 6 cut(s) 86, 252, 640, 649, 850, 1509
XbaI TCTAGA 1 cut(s) 842
XmiI GTMKAC 1 cut(s) 910
XmnI GAANNNNTTC 1 cut(s) 691
XspI CTAG 4 cut(s) 45, 345, 843, 1533
ZraI GACGTC 5 cut(s) 1091, 1118, 1145, 1199, 1226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.