pycom11g12100

Protein UPSTREAM OF FLC isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0006878
Physical Location & Seq
Forward (+)
11007359 .. 11010742
3384 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1593 bp
ATGGAGGGTAGGATGAAGAAGTACAGCCAAGTGAGTCCGGAAAGGGCGAAAGTGTGGACTGAGAAGTCGCCCAAGTACCACCAGAACCGCAAAGTTCCTGTGGTTTACTATCTCTCTCGAAATCGGCAGCTTGAACACCCCCATTTCATGGAGGTTCCTGTAACCTCTCCTCAAGGGCTCTACCTCAGAGATGTGATTAATAGGCTTAATGCTCTGAGGGGCAGAGGCATGGCTTCCATGTATTCATGGTCTTCTAAGAGAAGCTATAAGGGTGGATATGTTTGGCATGATCTCTCTGAAGATGATCTGATTCCTCCGGCCCATGGAAATGAGTACGTACTCAAAGGTTCTGAGCTTTTTGAAGAATCGAATTCAGGTCGGTTTAGCCCGTTGGCAGAAATAAAAATCCAGAGCTTGAAACAATTACCGGAACCTGCATCTTCTAGAAGCCAAGATGATTCTTCATCGTCGTCAAGTTTGAATGGGAAGGAAACAAAGCATTCTCAAGAAGATGAGCTCTCTCCTCCGGTTCAACGTCTCGAAGGCTCTTCCAGTGCATCTCCAGAGTCTACGGTTGGAAAGAATTCTTCGTGGGGCGGTTCACTGAGCTTGACAGAGTACAAGGTTTACAAGAGTGATGGTTTGGCTGATGCTTCTACGCAGACTGAAGAAAATGGGAACAAAACTAAAGCATCAGAAACATGTACAAGGGGTGTTTCCACAGATGATGGGTCGTTGGAACCGGATTGCAGTTATTATCATAATCGAGTGCTGTGTGTGAAAGAGAACTCTGAGATTTGCAGTGATTCAGTTTCCCCTCCTCGGTTTTCCCCCAGCCCATCTTCATCAGGTGGGAAGACTGAAACTTTGGAATCCCTTATTAGGGCTGATGTTAGTAAACTCAATAGTTTTAGAATAGTTGAAGGGGAAGACGTACGGATGCAAAACAATCCAAGGGTTAAGGCTACTAATGTACTTATGCAACTCATCTCGTGTGGTTCAATATCAGTGAAGGATCACAGTTTTGGTCTTATTCCGACCTACAAGGCCCAGTTTTCCCATTCCAATTTTCGCTCCCCATTATTCTCCACTAAAGTAATGCTGGGAGAGCTGGACTGCCTCTCAGAGAATCCAAGGCTAATGGGTTTGAGATTGGAAGATAAAGAATATTTTAGTGGGAGCTTGATTGAGACTAAAATGCTCAAGGAGGAAGATGGATGTGCAGCTTTAAAACGATCGTCTTCATACAATGCTGACAGGAGTTGTAAGAAGGTGGATTCAGTAGAGGACAAAGAGGAGTCTACATCAGGACGTTCGAAATGCATTCCAAGATCAGTCAAAGCTTCGCTGAACAAGCATCCACGAAGTGAATCTATGAGATTCCCCATTTCCGATAAGCCAAGGAACTCCTCAGATGTAGTTGATGGTGCACATATCAATACCCATAGCGTATCCAATGGATGTAGCAAAAGAATCACAGACCCTTCATCTCTTAAAAAGCAATCGAAAAGGTTAGATTCGTTTAGAGAAGAGAAGGAGAAGGAGAAGGTGATCAAAATCGAAGAAAGCTTGCTTCAGGAGCTCGGGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

531

Amino Acids

59.07

Weight (kDa)

8.34

Isoelectric Point (pI)

59.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOK PF06136 32 - 120 1.6e-49 SOSEKI protein DIX-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1591
AasI GACNNNNNNGTC 1 cut(s) 64
Acc36I ACCTGC 1 cut(s) 442
AccB7I CCANNNNNTGG 1 cut(s) 148
AccI GTMKAC 2 cut(s) 569, 1301
AccIII TCCGGA 1 cut(s) 37
AciI CCGC 2 cut(s) 88, 597
AclWI GGATC 1 cut(s) 1023
AcsI RAATTY 2 cut(s) 370, 583
AcuI CTGAAG 3 cut(s) 318, 687, 1559
AdeI CACNNNGTG 1 cut(s) 1367
AfaI GTAC 8 cut(s) 23, 77, 335, 339, 620, 706, 936, 975
AfiI CCNNNNNNNGG 5 cut(s) 148, 323, 822, 882, 883
AflIII ACRYGT 1 cut(s) 701
AgsI TTSAA 7 cut(s) 134, 362, 418, 481, 533, 923, 1002
Alw21I GWGCWC 3 cut(s) 519, 1432, 1584
Alw26I GTCTC 2 cut(s) 542, 1184
Alw44I GTGCAC 1 cut(s) 1428
AlwI GGATC 1 cut(s) 1023
Ama87I CYCGRG 1 cut(s) 1583
Aor13HI TCCGGA 1 cut(s) 37
AoxI GGCC 2 cut(s) 318, 1047
ApaLI GTGCAC 1 cut(s) 1428
ApeKI GCWGC 2 cut(s) 127, 1223
ApoI RAATTY 2 cut(s) 370, 583
ArsI GACNNNNNNTTYG 2 cut(s) 850, 882
AseI ATTAAT 1 cut(s) 198
Asp700I GAANNNNTTC 1 cut(s) 583
AspS9I GGNCC 2 cut(s) 319, 1048
AsuHPI GGTGA 1 cut(s) 1561
AsuII TTCGAA 1 cut(s) 1316
AvaI CYCGRG 1 cut(s) 1583
BaeGI GKGCMC 1 cut(s) 1432
BanII GRGCYC 3 cut(s) 180, 519, 1584
BauI CACGAG 1 cut(s) 991
BbsI GAAGAC 4 cut(s) 243, 863, 936, 1233
Bbv12I GWGCWC 3 cut(s) 519, 1432, 1584
BbvI GCAGC 2 cut(s) 139, 1235
BccI CCATC 5 cut(s) 632, 722, 847, 1208, 1418
BciVI GTATCC 1 cut(s) 1462
BclI TGATCA 1 cut(s) 1551
BcoDI GTCTC 2 cut(s) 542, 1184
BfaI CTAG 1 cut(s) 444
BfuAI ACCTGC 1 cut(s) 442
BfuI GTATCC 1 cut(s) 1462
BisI GCNGC 2 cut(s) 128, 1224
BlsI GCNGC 2 cut(s) 129, 1225
BmeT110I CYCGRG 1 cut(s) 1583
BmgT120I GGNCC 2 cut(s) 319, 1048
BmiI GGNNCC 3 cut(s) 156, 432, 741
BmrI ACTGGG 1 cut(s) 1045
BmsI GCATC 6 cut(s) 446, 566, 640, 701, 930, 1366
BmuI ACTGGG 1 cut(s) 1045
BpiI GAAGAC 4 cut(s) 243, 863, 936, 1233
BpmI CTGGAG 1 cut(s) 546
Bpu14I TTCGAA 1 cut(s) 1316
BpuEI CTTGAG 3 cut(s) 156, 489, 1187
BsaAI YACGTR 1 cut(s) 337
BsaBI GATNNNNATC 1 cut(s) 1556
BsaJI CCNNGG 5 cut(s) 322, 821, 953, 1133, 1400
BsaWI WCCGGW 4 cut(s) 37, 427, 526, 742
BsaXI ACNNNNNCTCC 1 cut(s) 1571
Bsc4I CCNNNNNNNGG 5 cut(s) 148, 323, 822, 882, 883
Bse1I ACTGG 2 cut(s) 552, 1051
Bse8I GATNNNNATC 1 cut(s) 1556
BseAI TCCGGA 1 cut(s) 37
BseDI CCNNGG 5 cut(s) 322, 821, 953, 1133, 1400
BseGI GGATG 5 cut(s) 18, 945, 1223, 1357, 1466
BseJI GATNNNNATC 1 cut(s) 1556
BseLI CCNNNNNNNGG 5 cut(s) 148, 323, 822, 882, 883
BseMII CTCAG 8 cut(s) 51, 199, 206, 342, 596, 783, 1137, 1425
BseNI ACTGG 2 cut(s) 552, 1051
BseRI GAGGAG 5 cut(s) 159, 513, 810, 1310, 1399
BseSI GKGCMC 1 cut(s) 1432
BseXI GCAGC 2 cut(s) 139, 1235
BseYI CCCAGC 2 cut(s) 833, 1102
BsgI GTGCAG 1 cut(s) 1242
Bsh1285I CGRYCG 1 cut(s) 1238
BshFI GGCC 2 cut(s) 320, 1049
BsiEI CGRYCG 1 cut(s) 1238
BsiHKAI GWGCWC 3 cut(s) 519, 1432, 1584
BsiHKCI CYCGRG 1 cut(s) 1583
BsiSI CCGG 5 cut(s) 38, 317, 428, 527, 743
BsiWI CGTACG 1 cut(s) 934
BslI CCNNNNNNNGG 5 cut(s) 148, 323, 822, 882, 883
BsmAI GTCTC 2 cut(s) 542, 1184
BsmBI CGTCTC 1 cut(s) 542
BsmI GAATGC 2 cut(s) 499, 1323
BsnI GGCC 2 cut(s) 320, 1049
BsoBI CYCGRG 1 cut(s) 1583
Bsp119I TTCGAA 1 cut(s) 1316
Bsp1286I GDGCHC 4 cut(s) 180, 519, 1432, 1584
Bsp13I TCCGGA 1 cut(s) 37
Bsp1407I TGTACA 1 cut(s) 704
Bsp143I GATC 6 cut(s) 289, 304, 1015, 1235, 1331, 1551
Bsp19I CCATGG 1 cut(s) 322
BspACI CCGC 2 cut(s) 88, 597
BspANI GGCC 2 cut(s) 320, 1049
BspCNI CTCAG 8 cut(s) 52, 198, 207, 343, 597, 784, 1136, 1424
BspEI TCCGGA 1 cut(s) 37
BspLI GGNNCC 3 cut(s) 156, 432, 741
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 1 cut(s) 1023
BspQI GCTCTTC 1 cut(s) 553
BspT104I TTCGAA 1 cut(s) 1316
BsrGI TGTACA 1 cut(s) 704
BsrI ACTGG 2 cut(s) 552, 1051
BssECI CCNNGG 5 cut(s) 322, 821, 953, 1133, 1400
BssMI GATC 6 cut(s) 289, 304, 1015, 1235, 1331, 1551
BssSI CACGAG 1 cut(s) 991
BssT1I CCWWGG 4 cut(s) 322, 953, 1133, 1400
Bst2BI CACGAG 1 cut(s) 991
Bst4CI ACNGT 2 cut(s) 574, 1022
Bst6I CTCTTC 2 cut(s) 553, 1524
BstAUI TGTACA 1 cut(s) 704
BstBAI YACGTR 1 cut(s) 337
BstBI TTCGAA 1 cut(s) 1316
BstC8I GCNNGC 1 cut(s) 1571
BstDEI CTNAG 9 cut(s) 60, 185, 215, 255, 351, 605, 792, 1123, 1411
BstDSI CCRYGG 1 cut(s) 322
BstF5I GGATG 5 cut(s) 18, 945, 1223, 1357, 1466
BstKTI GATC 6 cut(s) 292, 307, 1018, 1238, 1334, 1554
BstMAI GTCTC 2 cut(s) 542, 1184
BstMBI GATC 6 cut(s) 289, 304, 1015, 1235, 1331, 1551
BstMCI CGRYCG 1 cut(s) 1238
BstMWI GCNNNNNNNGC 3 cut(s) 1108, 1354, 1579
BstNSI RCATGY 1 cut(s) 705
BstSLI GKGCMC 1 cut(s) 1432
BstSNI TACGTA 1 cut(s) 337
BstV1I GCAGC 2 cut(s) 139, 1235
BstV2I GAAGAC 4 cut(s) 243, 863, 936, 1233
BsuI GTATCC 1 cut(s) 1462
BsuRI GGCC 2 cut(s) 320, 1049
BtgI CCRYGG 1 cut(s) 322
BtsCI GGATG 5 cut(s) 18, 945, 1223, 1357, 1466
BtsI GCAGTG 1 cut(s) 808
BtsIMutI CAGTG 4 cut(s) 559, 602, 808, 1014
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 1 cut(s) 1571
Cfr13I GGNCC 2 cut(s) 319, 1048
Csp6I GTAC 8 cut(s) 22, 76, 334, 338, 619, 705, 935, 974
CviAII CATG 7 cut(s) 148, 229, 238, 246, 287, 323, 702
CviQI GTAC 8 cut(s) 22, 76, 334, 338, 619, 705, 935, 974
DdeI CTNAG 9 cut(s) 60, 185, 215, 255, 351, 605, 792, 1123, 1411
DpnI GATC 6 cut(s) 291, 306, 1017, 1237, 1333, 1553
DpnII GATC 6 cut(s) 289, 304, 1015, 1235, 1331, 1551
DraI TTTAAA 1 cut(s) 1230
DraIII CACNNNGTG 1 cut(s) 1367
DrdI GACNNNNNNGTC 1 cut(s) 64
DseDI GACNNNNNNGTC 1 cut(s) 64
Eam1104I CTCTTC 2 cut(s) 553, 1524
EarI CTCTTC 2 cut(s) 553, 1524
Ecl136II GAGCTC 2 cut(s) 517, 1582
Eco105I TACGTA 1 cut(s) 337
Eco130I CCWWGG 4 cut(s) 322, 953, 1133, 1400
Eco24I GRGCYC 3 cut(s) 180, 519, 1584
Eco53kI GAGCTC 2 cut(s) 517, 1582
Eco57I CTGAAG 3 cut(s) 318, 687, 1559
Eco88I CYCGRG 1 cut(s) 1583
EcoICRI GAGCTC 2 cut(s) 517, 1582
EcoRI GAATTC 2 cut(s) 370, 583
EcoT14I CCWWGG 4 cut(s) 322, 953, 1133, 1400
EcoT22I ATGCAT 1 cut(s) 1325
EcoT38I GRGCYC 3 cut(s) 180, 519, 1584
ErhI CCWWGG 4 cut(s) 322, 953, 1133, 1400
Esp3I CGTCTC 1 cut(s) 542
FaeI CATG 7 cut(s) 151, 232, 241, 249, 290, 326, 705
FatI CATG 7 cut(s) 147, 228, 237, 245, 286, 322, 701
FbaI TGATCA 1 cut(s) 1551
FblI GTMKAC 2 cut(s) 569, 1301
Fnu4HI GCNGC 2 cut(s) 128, 1224
FokI GGATG 5 cut(s) 25, 952, 1230, 1344, 1473
FriOI GRGCYC 3 cut(s) 180, 519, 1584
Fsp4HI GCNGC 2 cut(s) 128, 1224
FspBI CTAG 1 cut(s) 444
GluI GCNGC 2 cut(s) 128, 1224
GsaI CCCAGC 2 cut(s) 837, 1106
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 2 cut(s) 320, 1049
HapII CCGG 5 cut(s) 38, 317, 428, 527, 743
Hin1II CATG 7 cut(s) 151, 232, 241, 249, 290, 326, 705
HindIII AAGCTT 2 cut(s) 1341, 1567
HpaII CCGG 5 cut(s) 38, 317, 428, 527, 743
HphI GGTGA 1 cut(s) 1561
Hpy166II GTNNAC 8 cut(s) 57, 106, 570, 602, 628, 899, 1302, 1430
Hpy188III TCNNGA 9 cut(s) 38, 117, 409, 444, 506, 539, 563, 1308, 1577
Hpy8I GTNNAC 8 cut(s) 57, 106, 570, 602, 628, 899, 1302, 1430
Hpy99I CGWCG 1 cut(s) 472
HpyAV CCTTC 9 cut(s) 481, 536, 917, 1006, 1264, 1494, 1528, 1534, 1540
HpyCH4III ACNGT 2 cut(s) 574, 1022
HpyCH4IV ACGT 4 cut(s) 336, 535, 933, 1312
HpyCH4V TGCA 9 cut(s) 437, 557, 750, 801, 943, 982, 1223, 1323, 1430
HpyF10VI GCNNNNNNNGC 3 cut(s) 1108, 1354, 1579
HpyF3I CTNAG 9 cut(s) 60, 185, 215, 255, 351, 605, 792, 1123, 1411
HpySE526I ACGT 4 cut(s) 336, 535, 933, 1312
Hsp92II CATG 7 cut(s) 151, 232, 241, 249, 290, 326, 705
Kpn2I TCCGGA 1 cut(s) 37
Ksp22I TGATCA 1 cut(s) 1551
Kzo9I GATC 6 cut(s) 289, 304, 1015, 1235, 1331, 1551
LguI GCTCTTC 1 cut(s) 553
LmnI GCTCC 3 cut(s) 1079, 1179, 1579
Lsp1109I GCAGC 2 cut(s) 139, 1235
LweI GCATC 6 cut(s) 446, 566, 640, 701, 930, 1366
MaeI CTAG 1 cut(s) 444
MaeII ACGT 4 cut(s) 336, 535, 933, 1312
MaeIII GTNAC 1 cut(s) 160
MalI GATC 6 cut(s) 291, 306, 1017, 1237, 1333, 1553
MboI GATC 6 cut(s) 289, 304, 1015, 1235, 1331, 1551
MhlI GDGCHC 4 cut(s) 180, 519, 1432, 1584
MluCI AATT 4 cut(s) 370, 422, 583, 1066
MlyI GAGTC 3 cut(s) 43, 575, 1307
MmeI TCCRAC 3 cut(s) 556, 717, 1061
Mph1103I ATGCAT 1 cut(s) 1325
MroI TCCGGA 1 cut(s) 37
MroXI GAANNNNTTC 1 cut(s) 583
MseI TTAA 5 cut(s) 198, 207, 960, 1229, 1494
MslI CAYNNNNRTG 1 cut(s) 327
MspI CCGG 5 cut(s) 38, 317, 428, 527, 743
Mva1269I GAATGC 2 cut(s) 499, 1323
MwoI GCNNNNNNNGC 3 cut(s) 1108, 1354, 1579
NcoI CCATGG 1 cut(s) 322
NdeII GATC 6 cut(s) 289, 304, 1015, 1235, 1331, 1551
NlaIII CATG 7 cut(s) 151, 232, 241, 249, 290, 326, 705
NlaIV GGNNCC 3 cut(s) 156, 432, 741
NsiI ATGCAT 1 cut(s) 1325
NspI RCATGY 1 cut(s) 705
NspV TTCGAA 1 cut(s) 1316
PciI ACATGT 1 cut(s) 701
PciSI GCTCTTC 1 cut(s) 553
PctI GAATGC 2 cut(s) 499, 1323
PdmI GAANNNNTTC 1 cut(s) 583
Pfl23II CGTACG 1 cut(s) 934
PflMI CCANNNNNTGG 1 cut(s) 148
PkrI GCNGC 2 cut(s) 129, 1225
Ple19I CGATCG 1 cut(s) 1238
PleI GAGTC 3 cut(s) 42, 574, 1306
PpsI GAGTC 3 cut(s) 42, 574, 1306
Ppu21I YACGTR 1 cut(s) 337
PscI ACATGT 1 cut(s) 701
PshBI ATTAAT 1 cut(s) 198
PsiI TTATAA 1 cut(s) 1591
Psp124BI GAGCTC 2 cut(s) 519, 1584
PspFI CCCAGC 2 cut(s) 833, 1102
PspLI CGTACG 1 cut(s) 934
PspN4I GGNNCC 3 cut(s) 156, 432, 741
PspPI GGNCC 2 cut(s) 319, 1048
PvuI CGATCG 1 cut(s) 1238
RsaI GTAC 8 cut(s) 23, 77, 335, 339, 620, 706, 936, 975
RsaNI GTAC 8 cut(s) 22, 76, 334, 338, 619, 705, 935, 974
RseI CAYNNNNRTG 1 cut(s) 327
SacI GAGCTC 2 cut(s) 519, 1584
SapI GCTCTTC 1 cut(s) 553
SaqAI TTAA 5 cut(s) 198, 207, 960, 1229, 1494
SatI GCNGC 2 cut(s) 128, 1224
Sau3AI GATC 6 cut(s) 289, 304, 1015, 1235, 1331, 1551
Sau96I GGNCC 2 cut(s) 319, 1048
SchI GAGTC 3 cut(s) 43, 575, 1307
SduI GDGCHC 4 cut(s) 180, 519, 1432, 1584
SfaNI GCATC 6 cut(s) 446, 566, 640, 701, 930, 1366
SfuI TTCGAA 1 cut(s) 1316
SmiMI CAYNNNNRTG 1 cut(s) 327
SmlI CTYRAG 3 cut(s) 171, 504, 1202
SmoI CTYRAG 3 cut(s) 171, 504, 1202
SnaBI TACGTA 1 cut(s) 337
Sse9I AATT 4 cut(s) 370, 422, 583, 1066
SsiI CCGC 2 cut(s) 88, 597
SspI AATATT 1 cut(s) 1169
SspMI CTAG 1 cut(s) 444
SstI GAGCTC 2 cut(s) 519, 1584
StyI CCWWGG 4 cut(s) 322, 953, 1133, 1400
TaaI ACNGT 2 cut(s) 574, 1022
TaiI ACGT 4 cut(s) 339, 538, 936, 1315
TaqI TCGA 7 cut(s) 118, 368, 540, 766, 1316, 1505, 1560
TasI AATT 4 cut(s) 370, 422, 583, 1066
TatI WGTACW 4 cut(s) 21, 618, 704, 973
Tru1I TTAA 5 cut(s) 198, 207, 960, 1229, 1494
Tru9I TTAA 5 cut(s) 198, 207, 960, 1229, 1494
TscAI CASTG 4 cut(s) 559, 609, 808, 1014
TseI GCWGC 2 cut(s) 127, 1223
TspDTI ATGAA 7 cut(s) 29, 136, 234, 453, 834, 1233, 1476
TspGWI ACGGA 1 cut(s) 952
TspRI CASTG 4 cut(s) 559, 609, 808, 1014
Van91I CCANNNNNTGG 1 cut(s) 148
VneI GTGCAC 1 cut(s) 1428
VspI ATTAAT 1 cut(s) 198
XapI RAATTY 2 cut(s) 370, 583
XbaI TCTAGA 1 cut(s) 443
XceI RCATGY 1 cut(s) 705
XmiI GTMKAC 2 cut(s) 569, 1301
XmnI GAANNNNTTC 1 cut(s) 583
XspI CTAG 1 cut(s) 444
Zsp2I ATGCAT 1 cut(s) 1325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.