pycom11g12550

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0016527
Physical Location & Seq
Reverse (-)
11468238 .. 11471501
3264 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1053 bp
ATGGGATCATTAGTTTCTTTTCTTCTTGTGATTATAGCACTTTCTCTGCAGGCAAATGGGCAGCCTTTGGTTCCTGCAATGTTCATATTTGGGGACTCAGTTGTGGATGCTGGTAACAACAACCACCTCTACACCCTGATCAAAGCCAACTTCCCTCCTTATGGAAGAGACTTTGTCAATCACAAGCCAACTGGGAGGTTCTGCAATGGAAAGCTTGCTTCAGACTTCACTGCTGAAAACCTTGGATTTACTTCTTACCCTCCAGCTTATCTTAGCAAAGAAGCCAATGGAAAAAAACTCTTGATTGGTGCCAATTTTGGCTCAGCTGGTTCTGGCTATTATGAGTATACAGCAAAATTATATAATGCAATTTCACTAAATCAGCAGCTGAAGTACTACAAGGAATACCAAAACAAGGTGGTGCGAATTGCAGCAGGAAATAAAGCCAATTTTACTTCAATAATATCTGATGCAATCTACCTTGTTGGTGCTGGGAGCAGTGACTTTCTTCAGAACTACTACATTGATCCTCTTCTTTACAAGGTCTATACGCCCAACCAGTTCTCTGACATTCTCATGCAATCTTATGCAACATTCATTCAGCATCTGTATGCATTGGGAGCAAGGAGAATCGGAGTGACCACATTGCCGCCACTTGGCTGCCTGCCGGCGGCCATCACTATATTTGGGTCGCACAGCAATGAGTGCGTGGATAAACTCAACAATAATGCAATCTCATTCAATAACAAGCTCAACGCCACATCTCAGAGCTTGCAAAATGGCCTCTCTGGTCTCAAGTTGGTCGTCTTAGATATCTACCAACCGCTCCATGACCTTGTCACAAAGCCTGCCGAAAATGGGTTTGCGGAGGCAAGGAGGGCTTGTTGTGGAACAGGGCTGGTAGAAACAGCAATACTGTGCAATGCAGACTCAGTAGGGACCTGCGCCAATGCGTCTGAGTATGTCTTTTGGGATGGCTTTCATCCTTCTGAGGCTGCAAACCAAATTTTGGCCAATGATTTGCTCAGCAGTGGCCTCTCCCTCATCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

351

Amino Acids

37.86

Weight (kDa)

6.58

Isoelectric Point (pI)

26.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 340 6.3e-37 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22810
fragaria_vesca FvH4_3g30030
malus_domestica MD11G1153800.v1.1
prunus_persica Prupe.6G116500_v2.0.a1 Prupe.6G116500_v2.0.a1
pyrus_communis pycom11g12550
rosa_chinensis RchiOBHm_Chr5g0055911
rosa_laevigata RLG00000035021
rosa_multiflora Rmu_sc0016879.1_g000001
rosa_roxburghii Rroxscaffold_1G00024440
rosa_samantha Rh5BG377000 Rh5CG400300 Rh5DG390500
rosa_wichuraiana Rw5G034390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 950
AccB1I GGYRCC 1 cut(s) 308
AccB7I CCANNNNNTGG 1 cut(s) 1009
AccBSI CCGCTC 1 cut(s) 826
AccI GTMKAC 1 cut(s) 347
AciI CCGC 4 cut(s) 650, 671, 824, 866
AclWI GGATC 2 cut(s) 13, 521
AcoI YGGCCR 2 cut(s) 672, 1011
AcsI RAATTY 1 cut(s) 1005
AcuI CTGAAG 3 cut(s) 204, 410, 494
AfaI GTAC 1 cut(s) 395
AfiI CCNNNNNNNGG 6 cut(s) 161, 415, 656, 670, 858, 1009
AgsI TTSAA 2 cut(s) 459, 742
AluBI AGCT 6 cut(s) 214, 266, 326, 388, 751, 771
AluI AGCT 6 cut(s) 214, 266, 326, 388, 751, 771
Alw26I GTCTC 2 cut(s) 162, 797
AlwI GGATC 2 cut(s) 13, 521
AlwNI CAGNNNCTG 2 cut(s) 388, 607
AoxI GGCC 4 cut(s) 672, 781, 1011, 1033
ApeKI GCWGC 5 cut(s) 61, 385, 431, 660, 995
ApoI RAATTY 1 cut(s) 1005
AspLEI GCGC 1 cut(s) 947
AspS9I GGNCC 1 cut(s) 939
AvaII GGWCC 1 cut(s) 939
BalI TGGCCA 1 cut(s) 1013
BanI GGYRCC 1 cut(s) 308
BbvI GCAGC 5 cut(s) 73, 397, 443, 647, 982
BccI CCATC 2 cut(s) 683, 968
BclI TGATCA 1 cut(s) 138
BcoDI GTCTC 2 cut(s) 162, 797
BfmI CTRYAG 1 cut(s) 47
BfuAI ACCTGC 1 cut(s) 950
BisI GCNGC 7 cut(s) 62, 386, 432, 650, 661, 672, 996
BlpI GCTNAGC 2 cut(s) 322, 1025
BlsI GCNGC 7 cut(s) 63, 387, 433, 651, 662, 673, 997
BmcAI AGTACT 1 cut(s) 395
Bme18I GGWCC 1 cut(s) 939
BmgT120I GGNCC 1 cut(s) 939
BmiI GGNNCC 3 cut(s) 72, 310, 940
BmrI ACTGGG 1 cut(s) 201
BmsI GCATC 3 cut(s) 97, 460, 613
BmuI ACTGGG 1 cut(s) 201
BpmI CTGGAG 1 cut(s) 246
Bpu1102I GCTNAGC 2 cut(s) 322, 1025
BpuEI CTTGAG 1 cut(s) 779
BsaI GGTCTC 1 cut(s) 797
BsaJI CCNNGG 1 cut(s) 241
BsaXI ACNNNNNCTCC 2 cut(s) 868, 898
Bsc4I CCNNNNNNNGG 6 cut(s) 161, 415, 656, 670, 858, 1009
Bse118I RCCGGY 1 cut(s) 667
Bse1I ACTGG 2 cut(s) 196, 559
Bse3DI GCAATG 5 cut(s) 84, 211, 644, 706, 928
BseDI CCNNGG 1 cut(s) 241
BseGI GGATG 3 cut(s) 112, 979, 982
BseLI CCNNNNNNNGG 6 cut(s) 161, 415, 656, 670, 858, 1009
BseMI GCAATG 5 cut(s) 84, 211, 644, 706, 928
BseMII CTCAG 7 cut(s) 111, 336, 779, 945, 948, 981, 1039
BseNI ACTGG 2 cut(s) 196, 559
BseXI GCAGC 5 cut(s) 73, 397, 443, 647, 982
BseYI CCCAGC 1 cut(s) 491
BshFI GGCC 4 cut(s) 674, 783, 1013, 1035
BshNI GGYRCC 1 cut(s) 308
BsiSI CCGG 1 cut(s) 668
BslFI GGGAC 2 cut(s) 107, 952
BslI CCNNNNNNNGG 6 cut(s) 161, 415, 656, 670, 858, 1009
BsmAI GTCTC 2 cut(s) 162, 797
BsmFI GGGAC 2 cut(s) 107, 952
BsnI GGCC 4 cut(s) 674, 783, 1013, 1035
Bso31I GGTCTC 1 cut(s) 797
Bsp143I GATC 3 cut(s) 5, 138, 526
Bsp1720I GCTNAGC 2 cut(s) 322, 1025
BspACI CCGC 4 cut(s) 650, 671, 824, 866
BspANI GGCC 4 cut(s) 674, 783, 1013, 1035
BspCNI CTCAG 7 cut(s) 110, 335, 778, 944, 949, 982, 1038
BspLI GGNNCC 3 cut(s) 72, 310, 940
BspMAI CTGCAG 1 cut(s) 51
BspMI ACCTGC 1 cut(s) 950
BspPI GGATC 2 cut(s) 13, 521
BspT107I GGYRCC 1 cut(s) 308
BspTNI GGTCTC 1 cut(s) 797
BsrBI CCGCTC 1 cut(s) 826
BsrDI GCAATG 5 cut(s) 84, 211, 644, 706, 928
BsrFI RCCGGY 1 cut(s) 667
BsrI ACTGG 2 cut(s) 196, 559
BssAI RCCGGY 1 cut(s) 667
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 3 cut(s) 5, 138, 526
BssNAI GTATAC 1 cut(s) 348
BssT1I CCWWGG 1 cut(s) 241
Bst1107I GTATAC 1 cut(s) 348
Bst4CI ACNGT 1 cut(s) 918
Bst6I CTCTTC 2 cut(s) 160, 537
BstAPI GCANNNNNTGC 1 cut(s) 705
BstC8I GCNNGC 6 cut(s) 51, 216, 665, 669, 773, 849
BstDEI CTNAG 9 cut(s) 97, 272, 322, 765, 808, 931, 957, 990, 1025
BstF5I GGATG 3 cut(s) 112, 979, 982
BstHHI GCGC 1 cut(s) 947
BstKTI GATC 3 cut(s) 8, 141, 529
BstMAI GTCTC 2 cut(s) 162, 797
BstMBI GATC 3 cut(s) 5, 138, 526
BstMWI GCNNNNNNNGC 3 cut(s) 620, 705, 878
BstSFI CTRYAG 1 cut(s) 47
BstV1I GCAGC 5 cut(s) 73, 397, 443, 647, 982
BstZ17I GTATAC 1 cut(s) 348
BsuRI GGCC 4 cut(s) 674, 783, 1013, 1035
BtsCI GGATG 3 cut(s) 112, 979, 982
BtsI GCAGTG 3 cut(s) 228, 505, 1036
BtsIMutI CAGTG 3 cut(s) 228, 505, 1036
BveI ACCTGC 1 cut(s) 950
Cac8I GCNNGC 6 cut(s) 51, 216, 665, 669, 773, 849
CaiI CAGNNNCTG 2 cut(s) 388, 607
CfoI GCGC 1 cut(s) 947
Cfr10I RCCGGY 1 cut(s) 667
Cfr13I GGNCC 1 cut(s) 939
CseI GACGC 1 cut(s) 942
Csp6I GTAC 1 cut(s) 394
CviAII CATG 2 cut(s) 577, 830
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 9 cut(s) 97, 272, 322, 765, 808, 931, 957, 990, 1025
DpnI GATC 3 cut(s) 7, 140, 528
DpnII GATC 3 cut(s) 5, 138, 526
EaeI YGGCCR 2 cut(s) 672, 1011
Eam1104I CTCTTC 2 cut(s) 160, 537
EarI CTCTTC 2 cut(s) 160, 537
Eco130I CCWWGG 1 cut(s) 241
Eco31I GGTCTC 1 cut(s) 797
Eco32I GATATC 1 cut(s) 814
Eco47I GGWCC 1 cut(s) 939
Eco57I CTGAAG 3 cut(s) 204, 410, 494
EcoO109I RGGNCCY 1 cut(s) 939
EcoRV GATATC 1 cut(s) 814
EcoT14I CCWWGG 1 cut(s) 241
EcoT22I ATGCAT 1 cut(s) 616
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 2 cut(s) 580, 833
FalI AAGNNNNNCTT 2 cut(s) 865, 897
FaqI GGGAC 2 cut(s) 107, 952
FatI CATG 2 cut(s) 576, 829
FbaI TGATCA 1 cut(s) 138
FblI GTMKAC 1 cut(s) 347
Fnu4HI GCNGC 7 cut(s) 62, 386, 432, 650, 661, 672, 996
FokI GGATG 3 cut(s) 119, 969, 986
Fsp4HI GCNGC 7 cut(s) 62, 386, 432, 650, 661, 672, 996
GlaI GCGC 1 cut(s) 946
GluI GCNGC 7 cut(s) 62, 386, 432, 650, 661, 672, 996
GsaI CCCAGC 1 cut(s) 495
GsuI CTGGAG 1 cut(s) 246
HaeIII GGCC 4 cut(s) 674, 783, 1013, 1035
HapII CCGG 1 cut(s) 668
HgaI GACGC 1 cut(s) 942
HhaI GCGC 1 cut(s) 947
Hin1II CATG 2 cut(s) 580, 833
Hin6I GCGC 1 cut(s) 945
HinP1I GCGC 1 cut(s) 945
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 3 cut(s) 95, 630, 929
HpaII CCGG 1 cut(s) 668
Hpy166II GTNNAC 1 cut(s) 348
Hpy188I TCNGA 8 cut(s) 223, 469, 513, 568, 635, 768, 958, 991
Hpy188III TCNNGA 1 cut(s) 301
Hpy8I GTNNAC 1 cut(s) 348
HpyAV CCTTC 1 cut(s) 996
HpyCH4III ACNGT 1 cut(s) 918
HpyF10VI GCNNNNNNNGC 3 cut(s) 620, 705, 878
HpyF3I CTNAG 9 cut(s) 97, 272, 322, 765, 808, 931, 957, 990, 1025
Hsp92II CATG 2 cut(s) 580, 833
HspAI GCGC 1 cut(s) 945
KroI GCCGGC 1 cut(s) 667
KroNI GCCGGC 1 cut(s) 669
Ksp22I TGATCA 1 cut(s) 138
Kzo9I GATC 3 cut(s) 5, 138, 526
LmnI GCTCC 3 cut(s) 495, 620, 831
Lsp1109I GCAGC 5 cut(s) 73, 397, 443, 647, 982
LweI GCATC 3 cut(s) 97, 460, 613
MaeIII GTNAC 4 cut(s) 113, 500, 637, 838
MalI GATC 3 cut(s) 7, 140, 528
MbiI CCGCTC 1 cut(s) 826
MboI GATC 3 cut(s) 5, 138, 526
MboII GAAGA 4 cut(s) 14, 177, 500, 524
MlsI TGGCCA 1 cut(s) 1013
MluCI AATT 6 cut(s) 313, 356, 369, 426, 448, 1005
MluNI TGGCCA 1 cut(s) 1013
MlyI GAGTC 2 cut(s) 89, 923
Mox20I TGGCCA 1 cut(s) 1013
Mph1103I ATGCAT 1 cut(s) 616
MroNI GCCGGC 1 cut(s) 667
MscI TGGCCA 1 cut(s) 1013
MslI CAYNNNNRTG 3 cut(s) 575, 609, 699
Msp20I TGGCCA 1 cut(s) 1013
MspA1I CMGCKG 2 cut(s) 326, 388
MspI CCGG 1 cut(s) 668
MwoI GCNNNNNNNGC 3 cut(s) 620, 705, 878
NaeI GCCGGC 1 cut(s) 669
NdeII GATC 3 cut(s) 5, 138, 526
NgoMIV GCCGGC 1 cut(s) 667
NlaIII CATG 2 cut(s) 580, 833
NlaIV GGNNCC 3 cut(s) 72, 310, 940
NmuCI GTSAC 3 cut(s) 500, 637, 838
NsiI ATGCAT 1 cut(s) 616
PdiI GCCGGC 1 cut(s) 669
PfeI GAWTC 1 cut(s) 630
PflFI GACNNNGTC 2 cut(s) 173, 836
PflMI CCANNNNNTGG 1 cut(s) 1009
PkrI GCNGC 7 cut(s) 63, 387, 433, 651, 662, 673, 997
PleI GAGTC 2 cut(s) 89, 923
PpsI GAGTC 2 cut(s) 89, 923
PpuMI RGGWCCY 1 cut(s) 939
Psp5II RGGWCCY 1 cut(s) 939
PspFI CCCAGC 1 cut(s) 491
PspN4I GGNNCC 3 cut(s) 72, 310, 940
PspPI GGNCC 1 cut(s) 939
PspPPI RGGWCCY 1 cut(s) 939
PstI CTGCAG 1 cut(s) 51
PstNI CAGNNNCTG 2 cut(s) 388, 607
PsyI GACNNNGTC 2 cut(s) 173, 836
PvuII CAGCTG 2 cut(s) 326, 388
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
RseI CAYNNNNRTG 3 cut(s) 575, 609, 699
SatI GCNGC 7 cut(s) 62, 386, 432, 650, 661, 672, 996
Sau3AI GATC 3 cut(s) 5, 138, 526
Sau96I GGNCC 1 cut(s) 939
ScaI AGTACT 1 cut(s) 395
SchI GAGTC 2 cut(s) 89, 923
SfaNI GCATC 3 cut(s) 97, 460, 613
SfcI CTRYAG 1 cut(s) 47
SinI GGWCC 1 cut(s) 939
SmiMI CAYNNNNRTG 3 cut(s) 575, 609, 699
SmlI CTYRAG 1 cut(s) 794
SmoI CTYRAG 1 cut(s) 794
Sse9I AATT 6 cut(s) 313, 356, 369, 426, 448, 1005
SsiI CCGC 4 cut(s) 650, 671, 824, 866
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 1 cut(s) 918
TasI AATT 6 cut(s) 313, 356, 369, 426, 448, 1005
TatI WGTACW 1 cut(s) 393
TauI GCSGC 2 cut(s) 652, 674
TfiI GAWTC 1 cut(s) 630
TscAI CASTG 3 cut(s) 235, 505, 1036
TseFI GTSAC 3 cut(s) 500, 637, 838
TseI GCWGC 5 cut(s) 61, 385, 431, 660, 995
Tsp45I GTSAC 3 cut(s) 500, 637, 838
TspDTI ATGAA 3 cut(s) 73, 586, 971
TspRI CASTG 3 cut(s) 235, 505, 1036
Tth111I GACNNNGTC 2 cut(s) 173, 836
Van91I CCANNNNNTGG 1 cut(s) 1009
VpaK11BI GGWCC 1 cut(s) 939
XapI RAATTY 1 cut(s) 1005
XmiI GTMKAC 1 cut(s) 347
ZrmI AGTACT 1 cut(s) 395
Zsp2I ATGCAT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.