pycom11g12720

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010479
Physical Location & Seq
Reverse (-)
11661911 .. 11662620
710 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 594 bp
ATGCCCAGCCCGAAGAGTTCTAGCCGGAGGGGGCAGCCGGAAGAGAAGAACCGCAGGCCGAGACCGCTCTCCGACAGATCATCGTCGTTCCACGGCCAGTTTCCCGAGGTGGCGACGCAGCAGCTTCGGAGGCCGAAAACGGTGCCGGACATGGCGTCGTTCAGGAACGCGGTGGGAACGATGGCGTCGTCGATGGAATTACGGCCAAAGCTGACGAAGCTGCTCCTCAACGTTACGATCCAGGGGAGCGTCGGGGCCATACAGGTCGTGATGTCGCCGGAACTGACGGTAGGAGATCTGGTCGCGGCGGCCGTGCGGCAATACGGGAAGGAAGGTCGCCGTCCGATCTTGCCTTCCGTCGAGCCGTCGTTGTTCGACCTCCATTACTCGCAGTTCAGCTTAGAAAGTTTGGATAGGGAGGAGAAGCTGATGGAGTTGGGATCCAGGAACTTTTTTCTGTGCCCTAACGCAAAAGCCGTGGGCGGTTGCGGTGGCGGTGGAGTGACGGCGTCGTCCGCGTCATGTTCAAAAGAGGCGGAGAAAGTTTCAAGGAATGGGTTTGCGTGGCTGAAGTTCATGAATTTCATGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

21.56

Weight (kDa)

9.83

Isoelectric Point (pI)

65.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 70 - 154 8.5e-38 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 511
AccB1I GGYRCC 1 cut(s) 142
AccBSI CCGCTC 1 cut(s) 67
AccII CGCG 3 cut(s) 170, 305, 518
AclI AACGTT 1 cut(s) 231
AclWI GGATC 3 cut(s) 232, 435, 448
AcoI YGGCCR 3 cut(s) 94, 203, 309
AcsI RAATTY 1 cut(s) 580
AcuI CTGAAG 1 cut(s) 590
AcyI GRCGYC 3 cut(s) 155, 185, 509
AgsI TTSAA 2 cut(s) 528, 549
AhdI GACNNNNNGTC 1 cut(s) 154
AjnI CCWGG 2 cut(s) 240, 443
AluBI AGCT 5 cut(s) 124, 211, 220, 399, 427
AluI AGCT 5 cut(s) 124, 211, 220, 399, 427
Alw26I GTCTC 1 cut(s) 55
AlwI GGATC 3 cut(s) 232, 435, 448
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 6 cut(s) 56, 94, 131, 203, 255, 309
ApeKI GCWGC 4 cut(s) 34, 118, 121, 220
ApoI RAATTY 1 cut(s) 580
AspS9I GGNCC 1 cut(s) 255
AvaI CYCGRG 1 cut(s) 104
BaeGI GKGCMC 1 cut(s) 464
BamHI GGATCC 1 cut(s) 440
BanI GGYRCC 1 cut(s) 142
BbvI GCAGC 4 cut(s) 46, 130, 133, 207
BccI CCATC 3 cut(s) 175, 187, 424
BceAI ACGGC 7 cut(s) 109, 218, 296, 324, 349, 461, 522
BcgI CGANNNNNNTGC 4 cut(s) 107, 124, 141, 158
BciT130I CCWGG 2 cut(s) 242, 445
BcoDI GTCTC 1 cut(s) 55
BfaI CTAG 1 cut(s) 21
BglII AGATCT 1 cut(s) 295
BisI GCNGC 7 cut(s) 35, 119, 122, 221, 306, 309, 317
BlsI GCNGC 7 cut(s) 36, 120, 123, 222, 307, 310, 318
Bme1390I CCNGG 2 cut(s) 242, 445
BmeRI GACNNNNNGTC 1 cut(s) 154
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 1 cut(s) 255
BmiI GGNNCC 3 cut(s) 144, 256, 442
BmrFI CCNGG 2 cut(s) 242, 445
BsaHI GRCGYC 3 cut(s) 155, 185, 509
BsaI GGTCTC 1 cut(s) 55
BsaJI CCNNGG 4 cut(s) 91, 105, 241, 477
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bse1I ACTGG 1 cut(s) 97
BseBI CCWGG 2 cut(s) 242, 445
BseDI CCNNGG 4 cut(s) 91, 105, 241, 477
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 2 cut(s) 215, 434
BseSI GKGCMC 1 cut(s) 464
BseX3I CGGCCG 1 cut(s) 309
BseXI GCAGC 4 cut(s) 46, 130, 133, 207
BseYI CCCAGC 1 cut(s) 5
Bsh1236I CGCG 3 cut(s) 170, 305, 518
Bsh1285I CGRYCG 1 cut(s) 312
BshFI GGCC 6 cut(s) 58, 96, 133, 205, 257, 311
BshNI GGYRCC 1 cut(s) 142
BsiEI CGRYCG 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 4 cut(s) 25, 38, 146, 278
BsmAI GTCTC 1 cut(s) 55
BsnI GGCC 6 cut(s) 58, 96, 133, 205, 257, 311
Bso31I GGTCTC 1 cut(s) 55
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 464
Bsp143I GATC 5 cut(s) 77, 237, 295, 345, 440
BspANI GGCC 6 cut(s) 58, 96, 133, 205, 257, 311
BspFNI CGCG 3 cut(s) 170, 305, 518
BspHI TCATGA 1 cut(s) 576
BspLI GGNNCC 3 cut(s) 144, 256, 442
BspPI GGATC 3 cut(s) 232, 435, 448
BspT107I GGYRCC 1 cut(s) 142
BspTNI GGTCTC 1 cut(s) 55
BsrBI CCGCTC 1 cut(s) 67
BsrI ACTGG 1 cut(s) 97
BssECI CCNNGG 4 cut(s) 91, 105, 241, 477
BssMI GATC 5 cut(s) 77, 237, 295, 345, 440
BssNI GRCGYC 3 cut(s) 155, 185, 509
Bst2UI CCWGG 2 cut(s) 242, 445
Bst4CI ACNGT 2 cut(s) 142, 289
Bst6I CTCTTC 2 cut(s) 8, 36
BstACI GRCGYC 3 cut(s) 155, 185, 509
BstC8I GCNNGC 1 cut(s) 56
BstDEI CTNAG 1 cut(s) 400
BstDSI CCRYGG 2 cut(s) 91, 477
BstFNI CGCG 3 cut(s) 170, 305, 518
BstKTI GATC 5 cut(s) 80, 240, 298, 348, 443
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 5 cut(s) 77, 237, 295, 345, 440
BstMCI CGRYCG 1 cut(s) 312
BstMWI GCNNNNNNNGC 4 cut(s) 64, 130, 217, 515
BstNI CCWGG 2 cut(s) 242, 445
BstSCI CCNGG 2 cut(s) 240, 443
BstSLI GKGCMC 1 cut(s) 464
BstUI CGCG 3 cut(s) 170, 305, 518
BstV1I GCAGC 4 cut(s) 46, 130, 133, 207
BstX2I RGATCY 2 cut(s) 295, 440
BstYI RGATCY 2 cut(s) 295, 440
BstZI CGGCCG 1 cut(s) 309
BsuRI GGCC 6 cut(s) 58, 96, 133, 205, 257, 311
BtgI CCRYGG 2 cut(s) 91, 477
Cac8I GCNNGC 1 cut(s) 56
CciI TCATGA 1 cut(s) 576
Cfr13I GGNCC 1 cut(s) 255
CseI GACGC 6 cut(s) 124, 144, 174, 238, 498, 507
CspCI CAANNNNNGTGG 2 cut(s) 459, 494
CviAII CATG 4 cut(s) 151, 522, 577, 586
DdeI CTNAG 1 cut(s) 400
DpnI GATC 5 cut(s) 79, 239, 297, 347, 442
DpnII GATC 5 cut(s) 77, 237, 295, 345, 440
DrdI GACNNNNNNGTC 1 cut(s) 511
DriI GACNNNNNGTC 1 cut(s) 154
DseDI GACNNNNNNGTC 1 cut(s) 511
EaeI YGGCCR 3 cut(s) 94, 203, 309
EagI CGGCCG 1 cut(s) 309
Eam1104I CTCTTC 2 cut(s) 8, 36
Eam1105I GACNNNNNGTC 1 cut(s) 154
EarI CTCTTC 2 cut(s) 8, 36
EciI GGCGGA 1 cut(s) 551
EclXI CGGCCG 1 cut(s) 309
Eco31I GGTCTC 1 cut(s) 55
Eco52I CGGCCG 1 cut(s) 309
Eco57I CTGAAG 1 cut(s) 590
Eco88I CYCGRG 1 cut(s) 104
EcoRII CCWGG 2 cut(s) 240, 443
FaeI CATG 4 cut(s) 154, 525, 580, 589
FaiI YATR 5 cut(s) 152, 260, 523, 578, 587
FatI CATG 4 cut(s) 150, 521, 576, 585
Fnu4HI GCNGC 7 cut(s) 35, 119, 122, 221, 306, 309, 317
Fsp4HI GCNGC 7 cut(s) 35, 119, 122, 221, 306, 309, 317
FspBI CTAG 1 cut(s) 21
GluI GCNGC 7 cut(s) 35, 119, 122, 221, 306, 309, 317
GsaI CCCAGC 1 cut(s) 9
HaeIII GGCC 6 cut(s) 58, 96, 133, 205, 257, 311
HapII CCGG 4 cut(s) 25, 38, 146, 278
HgaI GACGC 6 cut(s) 124, 144, 174, 238, 498, 507
Hin1I GRCGYC 3 cut(s) 155, 185, 509
Hin1II CATG 4 cut(s) 154, 525, 580, 589
HpaII CCGG 4 cut(s) 25, 38, 146, 278
Hpy188I TCNGA 3 cut(s) 73, 129, 345
Hpy188III TCNNGA 4 cut(s) 104, 163, 268, 577
Hpy99I CGWCG 9 cut(s) 88, 118, 160, 190, 193, 254, 362, 370, 514
HpyAV CCTTC 3 cut(s) 322, 326, 363
HpyCH4III ACNGT 2 cut(s) 142, 289
HpyCH4IV ACGT 1 cut(s) 231
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 130, 217, 515
HpyF3I CTNAG 1 cut(s) 400
HpySE526I ACGT 1 cut(s) 231
Hsp92I GRCGYC 3 cut(s) 155, 185, 509
Hsp92II CATG 4 cut(s) 154, 525, 580, 589
Kzo9I GATC 5 cut(s) 77, 237, 295, 345, 440
LmnI GCTCC 2 cut(s) 228, 246
Lsp1109I GCAGC 4 cut(s) 46, 130, 133, 207
MaeI CTAG 1 cut(s) 21
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 2 cut(s) 232, 502
MalI GATC 5 cut(s) 79, 239, 297, 347, 442
MbiI CCGCTC 1 cut(s) 67
MboI GATC 5 cut(s) 77, 237, 295, 345, 440
MboII GAAGA 3 cut(s) 25, 53, 58
MflI RGATCY 2 cut(s) 295, 440
MhlI GDGCHC 1 cut(s) 464
MluCI AATT 2 cut(s) 197, 580
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 7 cut(s) 21, 100, 123, 236, 389, 412, 526
MspI CCGG 4 cut(s) 25, 38, 146, 278
MspR9I CCNGG 2 cut(s) 242, 445
MvaI CCWGG 2 cut(s) 242, 445
MvnI CGCG 3 cut(s) 170, 305, 518
MwoI GCNNNNNNNGC 4 cut(s) 64, 130, 217, 515
NdeII GATC 5 cut(s) 77, 237, 295, 345, 440
NlaIII CATG 4 cut(s) 154, 525, 580, 589
NlaIV GGNNCC 3 cut(s) 144, 256, 442
NmeAIII GCCGAG 1 cut(s) 84
NmuCI GTSAC 1 cut(s) 502
PagI TCATGA 1 cut(s) 576
PcsI WCGNNNNNNNCGW 3 cut(s) 185, 309, 474
PflFI GACNNNGTC 1 cut(s) 508
PfoI TCCNGGA 1 cut(s) 443
PkrI GCNGC 7 cut(s) 36, 120, 123, 222, 307, 310, 318
Psp1406I AACGTT 1 cut(s) 231
Psp6I CCWGG 2 cut(s) 240, 443
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 2 cut(s) 240, 443
PspN4I GGNNCC 3 cut(s) 144, 256, 442
PspPI GGNCC 1 cut(s) 255
PsuI RGATCY 2 cut(s) 295, 440
PsyI GACNNNGTC 1 cut(s) 508
SatI GCNGC 7 cut(s) 35, 119, 122, 221, 306, 309, 317
Sau3AI GATC 5 cut(s) 77, 237, 295, 345, 440
Sau96I GGNCC 1 cut(s) 255
ScrFI CCNGG 2 cut(s) 242, 445
SduI GDGCHC 1 cut(s) 464
Sse9I AATT 2 cut(s) 197, 580
SspMI CTAG 1 cut(s) 21
StyD4I CCNGG 2 cut(s) 240, 443
TaaI ACNGT 2 cut(s) 142, 289
TaiI ACGT 1 cut(s) 234
TaqI TCGA 3 cut(s) 191, 360, 375
TasI AATT 2 cut(s) 197, 580
TauI GCSGC 3 cut(s) 308, 311, 319
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 4 cut(s) 34, 118, 121, 220
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 3 cut(s) 565, 574, 593
TspGWI ACGGA 1 cut(s) 346
Tth111I GACNNNGTC 1 cut(s) 508
XapI RAATTY 1 cut(s) 580
XspI CTAG 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.