pycom11g16820

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007213
Physical Location & Seq
Reverse (-)
18640608 .. 18645340
4733 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1047 bp
ATGCAGCCAAAAAACGAAGCTGAAAATCGACTACATGCTCATCCCATCCCACCAAGCGGTGTTTATTCGGAACCGTGGTGGTGCAGTGCTGGGCATAATCCCATCTCCCCAACGGGGACAGGGAGGAATGCAACCAACTCATCTTTGTTAGAATGCCCAAATGGTGGGTCAGAGTCTAATGACGGTCAATCTTTGTCCAATGATGAGACAAATGAAGATGATGATGATGCTACCAAAGATTCACATATTACATCTCCAAGATCAGCTGGAAATTATGGGCAAGATCACCGCAATGTAAAGCATGTTGGATCGGGTGTGCCAACAGTGCGCGATGATTGCCTTGCGCAACCACCACAGCTTGAACTTGTGGGTCACTCGATTGCTTGTGCTTCAAATCCTTACCAGGATCCTTATTATGGTGGAATGATGGCAGCTTATGGACATCAACCTTTGGGCTATCCCCCTTTTCTTGGAATGCCACATGCTAGAATGCCATTGCCTCTTGAGATGGCACAGGAGCCTGTGTATGTAAATGCAAAACAATACCAAGGGATTCTAAGGCGAAGACAGGCACGTGCAAAAGCAGAACTAGAAAGGAAGCTAATAAAAGTCAGGAAGCCATATCTTCATGAATCACGACATCAACATGCTATGAGAAGGGCGAGAGGTACTGGAGGACGTTTTGCAAAGAAAACTAATGGTGATAATTCAAACAGCACTAGACAAGAAAAGGGCAGAGGCTCTGGTCCAGCGCACTCATCACAATCCGGCAGTTCATCGGGCTCAGAACCATTTCCCTCGGATTCTGCTGAAACATGGAATTCCCCCAATAGTCAACGGGAAGCAAGAGGTTCCCAAGTGCACGATGCGTATGCAGCTCACAATTATGCAAATGGTAGTGGCTGCTACCAGCCCCATGGTGGTCTGCAGGCTTCAATGTATCCTTCATACTCGGGCAAGAGAGGGGAAGGAGGAGATTGGAAAGGCCAGCAACAGGGCAACATATCTTCGAACCAGGCCTCACAGAGGCGTCTTGCCATTCAGTGA

Protein Analysis

349

Amino Acids

37.81

Weight (kDa)

8.71

Isoelectric Point (pI)

58.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFB_NFYA PF02045 173 - 228 8.2e-27 CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 345
AccB7I CCANNNNNTGG 1 cut(s) 164
AccII CGCG 1 cut(s) 330
AciI CCGC 2 cut(s) 57, 289
AclWI GGATC 3 cut(s) 316, 401, 414
AcsI RAATTY 1 cut(s) 820
AcvI CACGTG 1 cut(s) 575
AcyI GRCGYC 1 cut(s) 1030
AfaI GTAC 1 cut(s) 670
AfiI CCNNNNNNNGG 8 cut(s) 56, 114, 164, 416, 470, 920, 994, 1026
AgsI TTSAA 4 cut(s) 362, 393, 711, 936
AjnI CCWGG 2 cut(s) 402, 1014
AluBI AGCT 6 cut(s) 20, 266, 358, 434, 601, 878
AluI AGCT 6 cut(s) 20, 266, 358, 434, 601, 878
Alw21I GWGCWC 1 cut(s) 864
Alw26I GTCTC 1 cut(s) 200
Alw44I GTGCAC 1 cut(s) 860
AlwI GGATC 3 cut(s) 316, 401, 414
Ama87I CYCGRG 1 cut(s) 952
AoxI GGCC 2 cut(s) 985, 1017
ApaLI GTGCAC 1 cut(s) 860
ApeKI GCWGC 4 cut(s) 4, 431, 875, 903
ApoI RAATTY 1 cut(s) 820
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
Asp700I GAANNNNTTC 1 cut(s) 792
AspLEI GCGC 3 cut(s) 330, 346, 754
AspS9I GGNCC 1 cut(s) 746
AsuHPI GGTGA 2 cut(s) 278, 713
AsuII TTCGAA 1 cut(s) 1010
AvaI CYCGRG 1 cut(s) 952
AvaII GGWCC 1 cut(s) 746
BaeGI GKGCMC 1 cut(s) 864
BamHI GGATCC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 785
BbrPI CACGTG 1 cut(s) 575
BbsI GAAGAC 1 cut(s) 571
Bbv12I GWGCWC 1 cut(s) 864
BbvI GCAGC 4 cut(s) 16, 443, 887, 890
BccI CCATC 4 cut(s) 53, 110, 421, 502
BcgI CGANNNNNNTGC 2 cut(s) 854, 888
BciT130I CCWGG 2 cut(s) 404, 1016
BciVI GTATCC 1 cut(s) 951
BcoDI GTCTC 1 cut(s) 200
BfaI CTAG 3 cut(s) 486, 590, 720
BfmI CTRYAG 1 cut(s) 926
BfuI GTATCC 1 cut(s) 951
BisI GCNGC 4 cut(s) 5, 432, 876, 904
BlsI GCNGC 4 cut(s) 6, 433, 877, 905
Bme1390I CCNGG 2 cut(s) 404, 1016
Bme18I GGWCC 1 cut(s) 746
BmeT110I CYCGRG 1 cut(s) 952
BmgT120I GGNCC 1 cut(s) 746
BmiI GGNNCC 4 cut(s) 72, 408, 519, 853
BmrFI CCNGG 2 cut(s) 404, 1016
BmsI GCATC 2 cut(s) 217, 856
BpiI GAAGAC 1 cut(s) 571
BpmI CTGGAG 1 cut(s) 693
Bpu14I TTCGAA 1 cut(s) 1010
BpuEI CTTGAG 1 cut(s) 524
BsaAI YACGTR 1 cut(s) 575
BsaHI GRCGYC 1 cut(s) 1030
BsaJI CCNNGG 4 cut(s) 74, 547, 798, 916
BsaXI ACNNNNNCTCC 2 cut(s) 509, 539
Bsc4I CCNNNNNNNGG 8 cut(s) 56, 114, 164, 416, 470, 920, 994, 1026
Bse1I ACTGG 1 cut(s) 676
Bse3DI GCAATG 2 cut(s) 298, 494
BseBI CCWGG 2 cut(s) 404, 1016
BseDI CCNNGG 4 cut(s) 74, 547, 798, 916
BseGI GGATG 2 cut(s) 40, 45
BseLI CCNNNNNNNGG 8 cut(s) 56, 114, 164, 416, 470, 920, 994, 1026
BseMI GCAATG 2 cut(s) 298, 494
BseMII CTCAG 1 cut(s) 798
BseNI ACTGG 1 cut(s) 676
BseRI GAGGAG 1 cut(s) 987
BseSI GKGCMC 1 cut(s) 864
BseXI GCAGC 4 cut(s) 16, 443, 887, 890
BseYI CCCAGC 1 cut(s) 89
BsgI GTGCAG 1 cut(s) 103
Bsh1236I CGCG 1 cut(s) 330
BshFI GGCC 2 cut(s) 987, 1019
BsiHKAI GWGCWC 1 cut(s) 864
BsiHKCI CYCGRG 1 cut(s) 952
BsiSI CCGG 1 cut(s) 768
BslFI GGGAC 1 cut(s) 130
BslI CCNNNNNNNGG 8 cut(s) 56, 114, 164, 416, 470, 920, 994, 1026
BsmAI GTCTC 1 cut(s) 200
BsmFI GGGAC 1 cut(s) 130
BsmI GAATGC 4 cut(s) 133, 158, 480, 495
BsnI GGCC 2 cut(s) 987, 1019
BsoBI CYCGRG 1 cut(s) 952
Bsp119I TTCGAA 1 cut(s) 1010
Bsp1286I GDGCHC 2 cut(s) 785, 864
Bsp143I GATC 4 cut(s) 260, 283, 308, 406
Bsp19I CCATGG 1 cut(s) 916
BspACI CCGC 2 cut(s) 57, 289
BspANI GGCC 2 cut(s) 987, 1019
BspCNI CTCAG 1 cut(s) 797
BspFNI CGCG 1 cut(s) 330
BspHI TCATGA 1 cut(s) 628
BspLI GGNNCC 4 cut(s) 72, 408, 519, 853
BspMAI CTGCAG 1 cut(s) 930
BspPI GGATC 3 cut(s) 316, 401, 414
BspT104I TTCGAA 1 cut(s) 1010
BsrDI GCAATG 2 cut(s) 298, 494
BsrI ACTGG 1 cut(s) 676
BssECI CCNNGG 4 cut(s) 74, 547, 798, 916
BssMI GATC 4 cut(s) 260, 283, 308, 406
BssNI GRCGYC 1 cut(s) 1030
BssT1I CCWWGG 2 cut(s) 547, 916
Bst2UI CCWGG 2 cut(s) 404, 1016
Bst4CI ACNGT 3 cut(s) 75, 185, 325
BstACI GRCGYC 1 cut(s) 1030
BstBAI YACGTR 1 cut(s) 575
BstBI TTCGAA 1 cut(s) 1010
BstC8I GCNNGC 2 cut(s) 930, 989
BstDEI CTNAG 2 cut(s) 557, 784
BstDSI CCRYGG 2 cut(s) 74, 916
BstENI CCTNNNNNAGG 1 cut(s) 1024
BstF5I GGATG 2 cut(s) 40, 45
BstFNI CGCG 1 cut(s) 330
BstHHI GCGC 3 cut(s) 330, 346, 754
BstKTI GATC 4 cut(s) 263, 286, 311, 409
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 4 cut(s) 260, 283, 308, 406
BstMWI GCNNNNNNNGC 3 cut(s) 325, 336, 875
BstNI CCWGG 2 cut(s) 404, 1016
BstNSI RCATGY 4 cut(s) 38, 305, 485, 650
BstSCI CCNGG 2 cut(s) 402, 1014
BstSFI CTRYAG 1 cut(s) 926
BstSLI GKGCMC 1 cut(s) 864
BstUI CGCG 1 cut(s) 330
BstV1I GCAGC 4 cut(s) 16, 443, 887, 890
BstV2I GAAGAC 1 cut(s) 571
BstX2I RGATCY 1 cut(s) 406
BstXI CCANNNNNNTGG 1 cut(s) 917
BstYI RGATCY 1 cut(s) 406
BsuI GTATCC 1 cut(s) 951
BsuRI GGCC 2 cut(s) 987, 1019
BtgI CCRYGG 2 cut(s) 74, 916
BtgZI GCGATG 1 cut(s) 345
BtsCI GGATG 2 cut(s) 40, 45
BtsI GCAGTG 1 cut(s) 91
BtsIMutI CAGTG 2 cut(s) 91, 330
Cac8I GCNNGC 2 cut(s) 930, 989
CciI TCATGA 1 cut(s) 628
CfoI GCGC 3 cut(s) 330, 346, 754
Cfr13I GGNCC 1 cut(s) 746
CseI GACGC 1 cut(s) 1019
Csp6I GTAC 1 cut(s) 669
CviAII CATG 7 cut(s) 35, 302, 482, 629, 647, 816, 917
CviQI GTAC 1 cut(s) 669
DdeI CTNAG 2 cut(s) 557, 784
DpnI GATC 4 cut(s) 262, 285, 310, 408
DpnII GATC 4 cut(s) 260, 283, 308, 406
Eco130I CCWWGG 2 cut(s) 547, 916
Eco147I AGGCCT 1 cut(s) 1019
Eco24I GRGCYC 1 cut(s) 785
Eco47I GGWCC 1 cut(s) 746
Eco72I CACGTG 1 cut(s) 575
Eco88I CYCGRG 1 cut(s) 952
EcoNI CCTNNNNNAGG 1 cut(s) 1024
EcoRI GAATTC 1 cut(s) 820
EcoRII CCWGG 2 cut(s) 402, 1014
EcoT14I CCWWGG 2 cut(s) 547, 916
EcoT38I GRGCYC 1 cut(s) 785
ErhI CCWWGG 2 cut(s) 547, 916
FaeI CATG 7 cut(s) 38, 305, 485, 632, 650, 819, 920
FaqI GGGAC 1 cut(s) 130
FatI CATG 7 cut(s) 34, 301, 481, 628, 646, 815, 916
Fnu4HI GCNGC 4 cut(s) 5, 432, 876, 904
FokI GGATG 2 cut(s) 27, 32
FriOI GRGCYC 1 cut(s) 785
Fsp4HI GCNGC 4 cut(s) 5, 432, 876, 904
FspBI CTAG 3 cut(s) 486, 590, 720
FspI TGCGCA 1 cut(s) 345
GlaI GCGC 3 cut(s) 329, 345, 753
GluI GCNGC 4 cut(s) 5, 432, 876, 904
GsaI CCCAGC 1 cut(s) 93
GsuI CTGGAG 1 cut(s) 693
HaeIII GGCC 2 cut(s) 987, 1019
HapII CCGG 1 cut(s) 768
HgaI GACGC 1 cut(s) 1019
HhaI GCGC 3 cut(s) 330, 346, 754
Hin1I GRCGYC 1 cut(s) 1030
Hin1II CATG 7 cut(s) 38, 305, 485, 632, 650, 819, 920
Hin6I GCGC 3 cut(s) 328, 344, 752
HinP1I GCGC 3 cut(s) 328, 344, 752
HincII GTYRAC 1 cut(s) 836
HindII GTYRAC 1 cut(s) 836
HinfI GANTC 5 cut(s) 173, 239, 553, 632, 803
HpaII CCGG 1 cut(s) 768
HphI GGTGA 2 cut(s) 278, 713
Hpy166II GTNNAC 2 cut(s) 836, 862
Hpy188I TCNGA 4 cut(s) 70, 172, 787, 802
Hpy188III TCNNGA 4 cut(s) 503, 613, 629, 636
Hpy8I GTNNAC 2 cut(s) 836, 862
HpyAV CCTTC 3 cut(s) 651, 954, 962
HpyCH4III ACNGT 3 cut(s) 75, 185, 325
HpyCH4IV ACGT 2 cut(s) 574, 679
HpyF10VI GCNNNNNNNGC 3 cut(s) 325, 336, 875
HpyF3I CTNAG 2 cut(s) 557, 784
HpySE526I ACGT 2 cut(s) 574, 679
Hsp92I GRCGYC 1 cut(s) 1030
Hsp92II CATG 7 cut(s) 38, 305, 485, 632, 650, 819, 920
HspAI GCGC 3 cut(s) 328, 344, 752
Kzo9I GATC 4 cut(s) 260, 283, 308, 406
LmnI GCTCC 1 cut(s) 517
Lsp1109I GCAGC 4 cut(s) 16, 443, 887, 890
LweI GCATC 2 cut(s) 217, 856
MaeI CTAG 3 cut(s) 486, 590, 720
MaeII ACGT 2 cut(s) 574, 679
MaeIII GTNAC 1 cut(s) 371
MalI GATC 4 cut(s) 262, 285, 310, 408
MboI GATC 4 cut(s) 260, 283, 308, 406
MboII GAAGA 4 cut(s) 227, 576, 617, 999
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 2 cut(s) 785, 864
MluCI AATT 4 cut(s) 271, 706, 820, 883
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 1 cut(s) 286
MroXI GAANNNNTTC 1 cut(s) 792
MslI CAYNNNNRTG 3 cut(s) 291, 645, 885
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 1 cut(s) 768
MspR9I CCNGG 2 cut(s) 404, 1016
Mva1269I GAATGC 4 cut(s) 133, 158, 480, 495
MvaI CCWGG 2 cut(s) 404, 1016
MvnI CGCG 1 cut(s) 330
MwoI GCNNNNNNNGC 3 cut(s) 325, 336, 875
NcoI CCATGG 1 cut(s) 916
NdeII GATC 4 cut(s) 260, 283, 308, 406
NlaIII CATG 7 cut(s) 38, 305, 485, 632, 650, 819, 920
NlaIV GGNNCC 4 cut(s) 72, 408, 519, 853
NmuCI GTSAC 1 cut(s) 371
NsbI TGCGCA 1 cut(s) 345
NspI RCATGY 4 cut(s) 38, 305, 485, 650
NspV TTCGAA 1 cut(s) 1010
PagI TCATGA 1 cut(s) 628
PceI AGGCCT 1 cut(s) 1019
PctI GAATGC 4 cut(s) 133, 158, 480, 495
PdmI GAANNNNTTC 1 cut(s) 792
PfeI GAWTC 4 cut(s) 239, 553, 632, 803
PflMI CCANNNNNTGG 1 cut(s) 164
PkrI GCNGC 4 cut(s) 6, 433, 877, 905
PleI GAGTC 1 cut(s) 181
PmaCI CACGTG 1 cut(s) 575
PmlI CACGTG 1 cut(s) 575
PpsI GAGTC 1 cut(s) 181
Ppu21I YACGTR 1 cut(s) 575
Psp6I CCWGG 2 cut(s) 402, 1014
PspCI CACGTG 1 cut(s) 575
PspFI CCCAGC 1 cut(s) 89
PspGI CCWGG 2 cut(s) 402, 1014
PspN4I GGNNCC 4 cut(s) 72, 408, 519, 853
PspPI GGNCC 1 cut(s) 746
PstI CTGCAG 1 cut(s) 930
PsuI RGATCY 1 cut(s) 406
PvuII CAGCTG 1 cut(s) 266
RsaI GTAC 1 cut(s) 670
RsaNI GTAC 1 cut(s) 669
RseI CAYNNNNRTG 3 cut(s) 291, 645, 885
SatI GCNGC 4 cut(s) 5, 432, 876, 904
Sau3AI GATC 4 cut(s) 260, 283, 308, 406
Sau96I GGNCC 1 cut(s) 746
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 404, 1016
SduI GDGCHC 2 cut(s) 785, 864
SfaNI GCATC 2 cut(s) 217, 856
SfcI CTRYAG 1 cut(s) 926
SfuI TTCGAA 1 cut(s) 1010
SinI GGWCC 1 cut(s) 746
SmiMI CAYNNNNRTG 3 cut(s) 291, 645, 885
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
Sse9I AATT 4 cut(s) 271, 706, 820, 883
SseBI AGGCCT 1 cut(s) 1019
SsiI CCGC 2 cut(s) 57, 289
SspMI CTAG 3 cut(s) 486, 590, 720
StuI AGGCCT 1 cut(s) 1019
StyD4I CCNGG 2 cut(s) 402, 1014
StyI CCWWGG 2 cut(s) 547, 916
TaaI ACNGT 3 cut(s) 75, 185, 325
TaiI ACGT 2 cut(s) 577, 682
TaqI TCGA 3 cut(s) 28, 377, 1010
TasI AATT 4 cut(s) 271, 706, 820, 883
TfiI GAWTC 4 cut(s) 239, 553, 632, 803
TscAI CASTG 2 cut(s) 91, 330
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 4 cut(s) 4, 431, 875, 903
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 5 cut(s) 228, 617, 645, 765, 936
TspRI CASTG 2 cut(s) 91, 330
Van91I CCANNNNNTGG 1 cut(s) 164
VneI GTGCAC 1 cut(s) 860
VpaK11BI GGWCC 1 cut(s) 746
XagI CCTNNNNNAGG 1 cut(s) 1024
XapI RAATTY 1 cut(s) 820
XceI RCATGY 4 cut(s) 38, 305, 485, 650
XcmI CCANNNNNNNNNTGG 1 cut(s) 917
XmnI GAANNNNTTC 1 cut(s) 792
XspI CTAG 3 cut(s) 486, 590, 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.