pycom11g16970

tRNA-specific 2-thiouridylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0009292
Physical Location & Seq
Forward (+)
18961274 .. 18966385
5112 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1755 bp
ATGAGAAACGCAGCTGCCATGGTCGTATGGCAATTGCTTCGCACCGTCTTATCTGGAAGCTCCTCCTCGTCATCCAGTGGTACAGCCATTTCCTCCCCTCCAAAACCAAAAACCCCAATTTCAAATCAGCACTCTTCCCCCAACCTCCACCTTCCGAAACGCTGTCGTTTACTGTATAATGTGTATGCTACGAAACCAATGGCGAGATTAATACCAATGGCCATGGCAGCCTCTCTACTCCCCCATATCACCTCCCACACTCTTCCTCTCAAACCCTATCTCTGGCCTTTTCCTCGCTCTCCAACCCCGACAACTCTCCTCTTCCTCAGACCCTTCTCTCTCTCCGCCCGCTCCGTCAACTGCTTCTCCGTCCCCCAAACTCCGCGCTCTCCAAACAGCTTCAGTGGAGATGTTGGCCCTTACTTGTCCTGTTCTATGCCCCATCAGAGCCTTAAAGTCGCCGTCTTGCTTAGCGGTGGCGTCGATAGCAGTGTCGCCCTCCGCCTCCTCCATGCCGCCGGTCACTCCTGCACCGCCTTCTACCTCAAAATTTGGTTCCAAGAAGACTTTGAGAACTTTTGGTCAGAATGCCCATGGGAAGAAGACTTGAAGTATGCAAAAGCTGTCTGCAATCAGGTTGATGTCCCACTAGAAGTTGTGCATTTGACGGATGAATATTGGAAGAATGTGGTTTCCTACATTATTGAAGAGTATCAATGTGGCAGAACTCCTAACCCCGATGTTCTTTGCAATACAAGAATAAAATTTGGTGCATTCATGGATTCCATCAACAGCATGAAGTTTGACTATGTGGCATCTGGGCATTACGCAAATGTTGTCCACCCACCTGCAAGTCAAACGGACAAAGATTCTGTTCTAGAACTCTCACAAGATATGGTGAAGGATCAAACATACTTCCTTTCAAATCTTTCTCAGGCCCAGCTTAGGAGATTAATATTCCCACTTGGTTGTCTGTCAAAGGATGAAGTTCGCAAACTTGCAACAAAGTTTGATCTGCCTAACAAAGACAGAAAGGATTCACAAGGAATATGCTTTCTAGGGAAGATTAAGTTCAGTGAATTTATAGCGAGACACATAGGGGAGAAGGAAGGTGTCATATTGGAAGCTGAGTCAGGAGATTTCCTAGGAAATCATCGGGGGTTTTGGTTCTACACAATTGGTCAACGACAAGGTCTACGCCTCCCTGGAGGACCCTGGTATGTTGTTGAGAAGGATATAAAAAACAATGTCGTTTTTGTGTCAAGAAATTACTTTTCAGTTGACAAAAGAAGGCGCCTGTTTCGTGTAGGCTCCTTGAAATGGCTAAATGGTTTGTCCCCAAACCAGATCAGTCAGCTAGAGTGCAAGGTTAGGCATGGGCCTGCCTTTTGTAAGTGCAGCTTTATAATGGAACCTGGTGAAGATGGTCATGAAGATGTTGCAGTTGTCCACTTGTCTCAAGATGATCAAGGCCTGGCAGCTGGGCAGTTTGCGGCCTTTTACCAAGGAAGAACCTGCTTGGGCTCCGGTGTAATTTTGGAGTCTTGGGATGATCAAGGTTTTCCCGTGTGTGCAAGAGCCCATGAAATTGCAAGAATGGAAGATAAATCACTTCTCGGTAAACCAGTTAAGATAAAAGTAGAGCCAGAGAATCTTTTAAGAGAGTCTGGTGAGGTAGATGGCGTGGAACTTTGCGGAGGACTGAAAAATCCTGGAATTGCTGCTACTAAACAACCAATGCAGGTATCTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko00564 ko01100 ko01110 ko04072 map00561 map00564 map01100 map01110 map04072
Pfam Domains
Protein Families
tRNA_Me_trans

Protein Analysis

585

Amino Acids

65.13

Weight (kDa)

8.56

Isoelectric Point (pI)

48.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_Me_trans PF03054 152 - 355 2.2e-78 tRNA methyl transferase HUP domain
tRNA_Me_trans_M PF20259 360 - 422 6.8e-26 tRNA methyl transferase PRC-barrel domain
tRNA_Me_trans_C PF20258 432 - 512 6.9e-17 Aminomethyltransferase beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1406
AarI CACCTGC 1 cut(s) 856
Acc36I ACCTGC 3 cut(s) 856, 1523, 1732
AccB1I GGYRCC 1 cut(s) 1293
AccBSI CCGCTC 1 cut(s) 351
AccI GTMKAC 1 cut(s) 1195
AccII CGCG 1 cut(s) 385
AciI CCGC 9 cut(s) 345, 349, 383, 474, 502, 516, 534, 1493, 1695
AclWI GGATC 1 cut(s) 912
AcoI YGGCCR 1 cut(s) 219
AcsI RAATTY 3 cut(s) 549, 764, 1079
AcuI CTGAAG 1 cut(s) 385
AcyI GRCGYC 2 cut(s) 480, 1294
AfaI GTAC 1 cut(s) 82
AfiI CCNNNNNNNGG 3 cut(s) 282, 945, 1320
AgsI TTSAA 5 cut(s) 123, 610, 707, 924, 1318
AjnI CCWGG 5 cut(s) 1204, 1214, 1414, 1473, 1711
AluBI AGCT 9 cut(s) 14, 60, 399, 623, 943, 1127, 1357, 1401, 1481
AluI AGCT 9 cut(s) 14, 60, 399, 623, 943, 1127, 1357, 1401, 1481
Alw26I GTCTC 2 cut(s) 1084, 1461
AlwI GGATC 1 cut(s) 912
AoxI GGCC 7 cut(s) 219, 284, 415, 936, 1379, 1471, 1494
ApeKI GCWGC 6 cut(s) 11, 14, 227, 1398, 1478, 1721
ApoI RAATTY 3 cut(s) 549, 764, 1079
AseI ATTAAT 2 cut(s) 209, 953
Asp700I GAANNNNTTC 1 cut(s) 1036
AspA2I CCTAGG 1 cut(s) 1144
AspLEI GCGC 2 cut(s) 387, 1296
AspS9I GGNCC 4 cut(s) 416, 937, 1211, 1379
AsuHPI GGTGA 4 cut(s) 241, 910, 1430, 1682
AvaII GGWCC 1 cut(s) 1211
AvrII CCTAGG 1 cut(s) 1144
BalI TGGCCA 1 cut(s) 221
BanI GGYRCC 1 cut(s) 1293
BanII GRGCYC 2 cut(s) 1526, 1582
BbsI GAAGAC 2 cut(s) 570, 609
BbvI GCAGC 5 cut(s) 23, 239, 1410, 1490, 1708
BccI CCATC 4 cut(s) 450, 794, 1418, 1673
BceAI ACGGC 1 cut(s) 446
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 5 cut(s) 1206, 1216, 1416, 1475, 1713
BclI TGATCA 2 cut(s) 1465, 1552
BcoDI GTCTC 2 cut(s) 1084, 1461
BfaI CTAG 5 cut(s) 650, 878, 1058, 1145, 1358
BfoI RGCGCY 1 cut(s) 1297
BfuAI ACCTGC 3 cut(s) 856, 1523, 1732
BisI GCNGC 8 cut(s) 12, 15, 228, 516, 1399, 1479, 1494, 1722
BlnI CCTAGG 1 cut(s) 1144
BlpI GCTNAGC 1 cut(s) 470
BlsI GCNGC 8 cut(s) 13, 16, 229, 517, 1400, 1480, 1495, 1723
Bme1390I CCNGG 5 cut(s) 1206, 1216, 1416, 1475, 1713
Bme18I GGWCC 1 cut(s) 1211
BmgT120I GGNCC 4 cut(s) 416, 937, 1211, 1379
BmiI GGNNCC 6 cut(s) 557, 1213, 1295, 1312, 1413, 1525
BmrFI CCNGG 5 cut(s) 1206, 1216, 1416, 1475, 1713
BmsI GCATC 1 cut(s) 824
BpiI GAAGAC 2 cut(s) 570, 609
BpmI CTGGAG 1 cut(s) 1227
Bpu10I CCTNAGC 1 cut(s) 944
Bpu1102I GCTNAGC 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 1443
BsaHI GRCGYC 2 cut(s) 480, 1294
BsaJI CCNNGG 7 cut(s) 18, 222, 593, 1144, 1204, 1214, 1504
BsaWI WCCGGW 1 cut(s) 1526
BsaXI ACNNNNNCTCC 2 cut(s) 350, 380
Bsc4I CCNNNNNNNGG 3 cut(s) 282, 945, 1320
Bse118I RCCGGY 1 cut(s) 518
Bse1I ACTGG 2 cut(s) 75, 1625
BseBI CCWGG 5 cut(s) 1206, 1216, 1416, 1475, 1713
BseDI CCNNGG 7 cut(s) 18, 222, 593, 1144, 1204, 1214, 1504
BseGI GGATG 4 cut(s) 71, 676, 988, 1555
BseLI CCNNNNNNNGG 3 cut(s) 282, 945, 1320
BseMII CTCAG 3 cut(s) 340, 947, 1119
BseNI ACTGG 2 cut(s) 75, 1625
BseRI GAGGAG 4 cut(s) 52, 55, 308, 497
BseXI GCAGC 5 cut(s) 23, 239, 1410, 1490, 1708
BseYI CCCAGC 2 cut(s) 939, 1481
BsgI GTGCAG 2 cut(s) 514, 1417
Bsh1236I CGCG 1 cut(s) 385
BshFI GGCC 7 cut(s) 221, 286, 417, 938, 1381, 1473, 1496
BshNI GGYRCC 1 cut(s) 1293
BsiSI CCGG 2 cut(s) 519, 1527
BslFI GGGAC 3 cut(s) 356, 629, 1321
BslI CCNNNNNNNGG 3 cut(s) 282, 945, 1320
BsmAI GTCTC 2 cut(s) 1084, 1461
BsmFI GGGAC 3 cut(s) 356, 629, 1321
BsmI GAATGC 2 cut(s) 593, 773
BsnI GGCC 7 cut(s) 221, 286, 417, 938, 1381, 1473, 1496
Bsp1286I GDGCHC 2 cut(s) 1526, 1582
Bsp143I GATC 5 cut(s) 904, 1012, 1347, 1465, 1552
Bsp1720I GCTNAGC 1 cut(s) 470
Bsp19I CCATGG 3 cut(s) 18, 222, 593
BspACI CCGC 9 cut(s) 345, 349, 383, 474, 502, 516, 534, 1493, 1695
BspANI GGCC 7 cut(s) 221, 286, 417, 938, 1381, 1473, 1496
BspCNI CTCAG 3 cut(s) 339, 946, 1120
BspFNI CGCG 1 cut(s) 385
BspHI TCATGA 1 cut(s) 1429
BspLI GGNNCC 6 cut(s) 557, 1213, 1295, 1312, 1413, 1525
BspMI ACCTGC 3 cut(s) 856, 1523, 1732
BspPI GGATC 1 cut(s) 912
BspT107I GGYRCC 1 cut(s) 1293
BsrBI CCGCTC 1 cut(s) 351
BsrFI RCCGGY 1 cut(s) 518
BsrI ACTGG 2 cut(s) 75, 1625
BssAI RCCGGY 1 cut(s) 518
BssECI CCNNGG 7 cut(s) 18, 222, 593, 1144, 1204, 1214, 1504
BssMI GATC 5 cut(s) 904, 1012, 1347, 1465, 1552
BssNI GRCGYC 2 cut(s) 480, 1294
BssT1I CCWWGG 5 cut(s) 18, 222, 593, 1144, 1504
Bst2UI CCWGG 5 cut(s) 1206, 1216, 1416, 1475, 1713
Bst4CI ACNGT 2 cut(s) 46, 174
Bst6I CTCTTC 4 cut(s) 139, 267, 326, 702
BstACI GRCGYC 2 cut(s) 480, 1294
BstC8I GCNNGC 2 cut(s) 349, 1383
BstDEI CTNAG 5 cut(s) 326, 470, 933, 944, 1128
BstDSI CCRYGG 3 cut(s) 18, 222, 593
BstF5I GGATG 4 cut(s) 71, 676, 988, 1555
BstFNI CGCG 1 cut(s) 385
BstH2I RGCGCY 1 cut(s) 1297
BstHHI GCGC 2 cut(s) 387, 1296
BstKTI GATC 5 cut(s) 907, 1015, 1350, 1468, 1555
BstMAI GTCTC 2 cut(s) 1084, 1461
BstMBI GATC 5 cut(s) 904, 1012, 1347, 1465, 1552
BstMWI GCNNNNNNNGC 2 cut(s) 227, 486
BstNI CCWGG 5 cut(s) 1206, 1216, 1416, 1475, 1713
BstSCI CCNGG 5 cut(s) 1204, 1214, 1414, 1473, 1711
BstUI CGCG 1 cut(s) 385
BstV1I GCAGC 5 cut(s) 23, 239, 1410, 1490, 1708
BstV2I GAAGAC 2 cut(s) 570, 609
BsuRI GGCC 7 cut(s) 221, 286, 417, 938, 1381, 1473, 1496
BtgI CCRYGG 3 cut(s) 18, 222, 593
BtsCI GGATG 4 cut(s) 71, 676, 988, 1555
BtsI GCAGTG 1 cut(s) 496
BtsIMutI CAGTG 4 cut(s) 82, 409, 496, 1081
BveI ACCTGC 3 cut(s) 856, 1523, 1732
Cac8I GCNNGC 2 cut(s) 349, 1383
CciI TCATGA 1 cut(s) 1429
CfoI GCGC 2 cut(s) 387, 1296
Cfr10I RCCGGY 1 cut(s) 518
Cfr13I GGNCC 4 cut(s) 416, 937, 1211, 1379
CseI GACGC 1 cut(s) 469
CsiI ACCWGGT 1 cut(s) 1414
Csp6I GTAC 1 cut(s) 81
CviAII CATG 9 cut(s) 19, 223, 512, 594, 778, 796, 1376, 1430, 1583
CviQI GTAC 1 cut(s) 81
DdeI CTNAG 5 cut(s) 326, 470, 933, 944, 1128
DinI GGCGCC 1 cut(s) 1295
DpnI GATC 5 cut(s) 906, 1014, 1349, 1467, 1554
DpnII GATC 5 cut(s) 904, 1012, 1347, 1465, 1552
EaeI YGGCCR 1 cut(s) 219
Eam1104I CTCTTC 4 cut(s) 139, 267, 326, 702
EarI CTCTTC 4 cut(s) 139, 267, 326, 702
EciI GGCGGA 2 cut(s) 334, 491
Eco130I CCWWGG 5 cut(s) 18, 222, 593, 1144, 1504
Eco147I AGGCCT 1 cut(s) 1473
Eco24I GRGCYC 2 cut(s) 1526, 1582
Eco47I GGWCC 1 cut(s) 1211
Eco57I CTGAAG 1 cut(s) 385
EcoO109I RGGNCCY 1 cut(s) 1211
EcoRII CCWGG 5 cut(s) 1204, 1214, 1414, 1473, 1711
EcoT14I CCWWGG 5 cut(s) 18, 222, 593, 1144, 1504
EcoT38I GRGCYC 2 cut(s) 1526, 1582
EgeI GGCGCC 1 cut(s) 1295
EheI GGCGCC 1 cut(s) 1295
ErhI CCWWGG 5 cut(s) 18, 222, 593, 1144, 1504
FaeI CATG 9 cut(s) 22, 226, 515, 597, 781, 799, 1379, 1433, 1586
FalI AAGNNNNNCTT 2 cut(s) 1385, 1417
FaqI GGGAC 3 cut(s) 356, 629, 1321
FatI CATG 9 cut(s) 18, 222, 511, 593, 777, 795, 1375, 1429, 1582
FauI CCCGC 1 cut(s) 356
FbaI TGATCA 2 cut(s) 1465, 1552
FblI GTMKAC 1 cut(s) 1195
Fnu4HI GCNGC 8 cut(s) 12, 15, 228, 516, 1399, 1479, 1494, 1722
FokI GGATG 4 cut(s) 58, 683, 995, 1562
FriOI GRGCYC 2 cut(s) 1526, 1582
Fsp4HI GCNGC 8 cut(s) 12, 15, 228, 516, 1399, 1479, 1494, 1722
FspBI CTAG 5 cut(s) 650, 878, 1058, 1145, 1358
GlaI GCGC 2 cut(s) 386, 1295
GluI GCNGC 8 cut(s) 12, 15, 228, 516, 1399, 1479, 1494, 1722
GsaI CCCAGC 2 cut(s) 943, 1485
GsuI CTGGAG 1 cut(s) 1227
HaeII RGCGCY 1 cut(s) 1297
HaeIII GGCC 7 cut(s) 221, 286, 417, 938, 1381, 1473, 1496
HapII CCGG 2 cut(s) 519, 1527
HgaI GACGC 1 cut(s) 469
HhaI GCGC 2 cut(s) 387, 1296
Hin1I GRCGYC 2 cut(s) 480, 1294
Hin1II CATG 9 cut(s) 22, 226, 515, 597, 781, 799, 1379, 1433, 1586
Hin6I GCGC 2 cut(s) 385, 1294
HinP1I GCGC 2 cut(s) 385, 1294
HincII GTYRAC 3 cut(s) 358, 1184, 1282
HindII GTYRAC 3 cut(s) 358, 1184, 1282
HinfI GANTC 7 cut(s) 782, 869, 1037, 1130, 1541, 1651, 1664
HpaII CCGG 2 cut(s) 519, 1527
HphI GGTGA 4 cut(s) 241, 910, 1430, 1682
Hpy166II GTNNAC 8 cut(s) 170, 358, 841, 1184, 1196, 1282, 1450, 1622
Hpy188I TCNGA 4 cut(s) 156, 329, 447, 586
Hpy188III TCNNGA 6 cut(s) 54, 878, 1134, 1263, 1430, 1460
Hpy8I GTNNAC 8 cut(s) 170, 358, 841, 1184, 1196, 1282, 1450, 1622
Hpy99I CGWCG 1 cut(s) 485
HpyAV CCTTC 8 cut(s) 161, 343, 547, 895, 1099, 1103, 1225, 1284
HpyCH4III ACNGT 2 cut(s) 46, 174
HpyF10VI GCNNNNNNNGC 2 cut(s) 227, 486
HpyF3I CTNAG 5 cut(s) 326, 470, 933, 944, 1128
Hsp92I GRCGYC 2 cut(s) 480, 1294
Hsp92II CATG 9 cut(s) 22, 226, 515, 597, 781, 799, 1379, 1433, 1586
HspAI GCGC 2 cut(s) 385, 1294
KasI GGCGCC 1 cut(s) 1293
Ksp22I TGATCA 2 cut(s) 1465, 1552
Kzo9I GATC 5 cut(s) 904, 1012, 1347, 1465, 1552
LmnI GCTCC 4 cut(s) 65, 356, 1316, 1529
Lsp1109I GCAGC 5 cut(s) 23, 239, 1410, 1490, 1708
LweI GCATC 1 cut(s) 824
MabI ACCWGGT 1 cut(s) 1414
MaeI CTAG 5 cut(s) 650, 878, 1058, 1145, 1358
MaeIII GTNAC 1 cut(s) 521
MalI GATC 5 cut(s) 906, 1014, 1349, 1467, 1554
MbiI CCGCTC 1 cut(s) 351
MboI GATC 5 cut(s) 904, 1012, 1347, 1465, 1552
MfeI CAATTG 2 cut(s) 32, 1176
MhlI GDGCHC 2 cut(s) 1526, 1582
MlsI TGGCCA 1 cut(s) 221
MluNI TGGCCA 1 cut(s) 221
Mly113I GGCGCC 1 cut(s) 1294
MlyI GAGTC 3 cut(s) 1139, 1550, 1673
MmeI TCCRAC 1 cut(s) 326
Mox20I TGGCCA 1 cut(s) 221
MroXI GAANNNNTTC 1 cut(s) 1036
MscI TGGCCA 1 cut(s) 221
MseI TTAA 6 cut(s) 209, 453, 953, 1068, 1629, 1658
MslI CAYNNNNRTG 1 cut(s) 1434
Msp20I TGGCCA 1 cut(s) 221
MspA1I CMGCKG 2 cut(s) 14, 1481
MspI CCGG 2 cut(s) 519, 1527
MspR9I CCNGG 5 cut(s) 1206, 1216, 1416, 1475, 1713
MunI CAATTG 2 cut(s) 32, 1176
Mva1269I GAATGC 2 cut(s) 593, 773
MvaI CCWGG 5 cut(s) 1206, 1216, 1416, 1475, 1713
MvnI CGCG 1 cut(s) 385
MwoI GCNNNNNNNGC 2 cut(s) 227, 486
NarI GGCGCC 1 cut(s) 1294
NcoI CCATGG 3 cut(s) 18, 222, 593
NdeII GATC 5 cut(s) 904, 1012, 1347, 1465, 1552
NlaIII CATG 9 cut(s) 22, 226, 515, 597, 781, 799, 1379, 1433, 1586
NlaIV GGNNCC 6 cut(s) 557, 1213, 1295, 1312, 1413, 1525
NmuCI GTSAC 1 cut(s) 521
PagI TCATGA 1 cut(s) 1429
PaqCI CACCTGC 1 cut(s) 856
PceI AGGCCT 1 cut(s) 1473
PctI GAATGC 2 cut(s) 593, 773
PdmI GAANNNNTTC 1 cut(s) 1036
PfeI GAWTC 4 cut(s) 782, 869, 1037, 1651
PflFI GACNNNGTC 1 cut(s) 1191
PfoI TCCNGGA 1 cut(s) 1711
PkrI GCNGC 8 cut(s) 13, 16, 229, 517, 1400, 1480, 1495, 1723
PleI GAGTC 3 cut(s) 1138, 1549, 1672
PluTI GGCGCC 1 cut(s) 1297
PpsI GAGTC 3 cut(s) 1138, 1549, 1672
PpuMI RGGWCCY 1 cut(s) 1211
PshBI ATTAAT 2 cut(s) 209, 953
PsiI TTATAA 1 cut(s) 1406
Psp5II RGGWCCY 1 cut(s) 1211
Psp6I CCWGG 5 cut(s) 1204, 1214, 1414, 1473, 1711
PspFI CCCAGC 2 cut(s) 939, 1481
PspGI CCWGG 5 cut(s) 1204, 1214, 1414, 1473, 1711
PspN4I GGNNCC 6 cut(s) 557, 1213, 1295, 1312, 1413, 1525
PspPI GGNCC 4 cut(s) 416, 937, 1211, 1379
PspPPI RGGWCCY 1 cut(s) 1211
PsyI GACNNNGTC 1 cut(s) 1191
PvuII CAGCTG 2 cut(s) 14, 1481
RsaI GTAC 1 cut(s) 82
RsaNI GTAC 1 cut(s) 81
RseI CAYNNNNRTG 1 cut(s) 1434
SaqAI TTAA 6 cut(s) 209, 453, 953, 1068, 1629, 1658
SatI GCNGC 8 cut(s) 12, 15, 228, 516, 1399, 1479, 1494, 1722
Sau3AI GATC 5 cut(s) 904, 1012, 1347, 1465, 1552
Sau96I GGNCC 4 cut(s) 416, 937, 1211, 1379
SchI GAGTC 3 cut(s) 1139, 1550, 1673
ScrFI CCNGG 5 cut(s) 1206, 1216, 1416, 1475, 1713
SduI GDGCHC 2 cut(s) 1526, 1582
SexAI ACCWGGT 1 cut(s) 1414
SfaNI GCATC 1 cut(s) 824
SfoI GGCGCC 1 cut(s) 1295
SinI GGWCC 1 cut(s) 1211
SmiMI CAYNNNNRTG 1 cut(s) 1434
SmlI CTYRAG 1 cut(s) 1458
SmoI CTYRAG 1 cut(s) 1458
SseBI AGGCCT 1 cut(s) 1473
SsiI CCGC 9 cut(s) 345, 349, 383, 474, 502, 516, 534, 1493, 1695
SspDI GGCGCC 1 cut(s) 1293
SspI AATATT 2 cut(s) 677, 957
SspMI CTAG 5 cut(s) 650, 878, 1058, 1145, 1358
StuI AGGCCT 1 cut(s) 1473
StyD4I CCNGG 5 cut(s) 1204, 1214, 1414, 1473, 1711
StyI CCWWGG 5 cut(s) 18, 222, 593, 1144, 1504
TaaI ACNGT 2 cut(s) 46, 174
TaqI TCGA 1 cut(s) 483
TauI GCSGC 2 cut(s) 518, 1496
TfiI GAWTC 4 cut(s) 782, 869, 1037, 1651
Tru1I TTAA 6 cut(s) 209, 453, 953, 1068, 1629, 1658
Tru9I TTAA 6 cut(s) 209, 453, 953, 1068, 1629, 1658
TscAI CASTG 4 cut(s) 82, 409, 496, 1081
TseFI GTSAC 1 cut(s) 521
TseI GCWGC 6 cut(s) 11, 14, 227, 1398, 1478, 1721
Tsp45I GTSAC 1 cut(s) 521
TspDTI ATGAA 6 cut(s) 687, 766, 812, 999, 1446, 1599
TspGWI ACGGA 4 cut(s) 343, 358, 683, 875
TspRI CASTG 4 cut(s) 82, 409, 496, 1081
Tth111I GACNNNGTC 1 cut(s) 1191
VpaK11BI GGWCC 1 cut(s) 1211
VspI ATTAAT 2 cut(s) 209, 953
XapI RAATTY 3 cut(s) 549, 764, 1079
XbaI TCTAGA 1 cut(s) 877
XmaJI CCTAGG 1 cut(s) 1144
XmiI GTMKAC 1 cut(s) 1195
XmnI GAANNNNTTC 1 cut(s) 1036
XspI CTAG 5 cut(s) 650, 878, 1058, 1145, 1358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.