pycom11g17530

Pentatricopeptide repeat-containing protein At1g66345

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0011738
Physical Location & Seq
Reverse (-)
19780363 .. 19781961
1599 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1599 bp
ATGGCTTCTCTGCTGCGTCGAATTGCACAATCTTTAATTCCCAAACCCATCAATCAGAAAACCCAACTGATCCACACAAGCTCAGGGCCGCCAAATGACGTCGTCAAATCCATCCGCGACTCGTTCAGAAGCAGCTGCAATTGGGACACTCTGTCCACAAAGTTCGAGTCCGTTAAATTGGACGGAGAGCTCGTCGAGAGTGTGCTGCTAGAACTGAAGGAACCCATCGATGCGAAACGTGCTCTGGGTTTCTTCCACTGGGCAGCTCACAGCAAAGATTTCGAACATGGGGTTTGGTCCTACTCCATAACCATTCATATTTTAGCCAGAGCCAGGCTGCTCATGGATGCAAGAGCATTGCTCGAGTCGGTTTTGAAGAAAAATGTTGGAAATGCTTCAAAGTTTTCGGTTGTGGATTCGTTGCTTAGTAGTTACGAGGTCGCGGCTTCAAGTCCGTTTGTGTTTGATTTGCTGGTGCAGGCTTACGCTAAGCTGAGAATGTTTGAGACTGGTTTTGATATTTGTTGTTATTTGGGGGAACGTGGGTTGCCTTTGAGTCTCACAAGTTACAACACATTGCTTCGTGTCGTCGAAAAATCTGACCGGACTGCTCTGGTTTGGAAGATTTATGAGCATATGATCGAAAACAGAAGGTATCCGAACGAAGAGACGATTAAAATCTTGGTTGATGCTTTGTGCAAGGAAGGGAAGTTGAGGAAATGTGTTGACATGCTGGACAGGATTCATGGGAAGAGATGCTCGCCTTCCGTGGTTGCGAATACTAGTTTAGTTTTCGGGATTTTGGAGGAGGGTAGGGTTGAGCAGGGAATGGTGGTATTGAAGAAAATGTTGCAAAGAAATATGGTTCTTGATACAATTGCTTACTCATTGACTGTATATGCTAAGGTTAAACTAGGAGATGTAAACTCCGCACGGGAGGTGTATGAAGAAATGCTTAAGAGAGGTTTTCGACCAAACTCTTTCATCTGTACCTTGTTTATAGGAGCTCATTGTGAAGGAGGACGAATTGACGAAGCACAATGCATCATGCACGAGATGCAAACTATGGATTTGAAGCCGTATGACGAGACTTACAATTTTCTTATTGAAGGATGTTGCAAAGCCGGAAGAGTGGAAGCAAGCACGAGTTACTTGAAGAAGATGATGGAGTGCGGGTTCATCCCTAGTTGTTCTTCCTTCAACGAGATGGTTGGAAAGTTGTGTGAAACTGGAGATGCAGAGCATGCCAATGAAATGTTAACTACCCTGTTAGATAAGGGGTTTGTACCCGACGAGATTACGTACTCTCATCTGATCAACGGGTTTGAGAGAAAAGGTGACAATCAGGAAGTTGTCAAGCTCTACTATGAGATGGAGTCGAGGTCGCTGTTTCCTGGAATTTCGGTTTTTACATCCTTGATAAAGAGCTTCTGCCAAACAGGGAAACCGGAGGAAGCAGAAAAGTATATTGGATTCATGAAAGGTCGTTCGATAGCCCCGAGTTTATGTACATATGAGCTCCTGATTTCCAGCCACTTGGAGAAGGGCAATTTAGACAGGGCCCTTCACCTTCGGAAAGAAATGGCCAAAAAGAATTAA

Protein Analysis

533

Amino Acids

60.17

Weight (kDa)

7.5

Isoelectric Point (pI)

32.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 188 - 234 1.8e-07 PPR repeat family
PPR_long PF17177 303 - 451 2.9e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 325 - 374 2.1e-09 PPR repeat family
PPR_1 PF12854 357 - 388 5.8e-06 PPR repeat
PPR_2 PF13041 364 - 408 2.8e-06 PPR repeat family
PPR PF01535 364 - 393 3.3e-06 PPR repeat
PPR_2 PF13041 397 - 442 4.5e-07 PPR repeat family
PPR_3 PF13812 454 - 510 2.9e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 465 - 510 5.7e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 102
AccII CGCG 2 cut(s) 117, 443
AciI CCGC 5 cut(s) 89, 115, 443, 930, 1173
AclWI GGATC 1 cut(s) 64
AcoI YGGCCR 1 cut(s) 1584
AcsI RAATTY 1 cut(s) 1398
AcuI CTGAAG 1 cut(s) 236
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 4 cut(s) 991, 1287, 1304, 1510
AfiI CCNNNNNNNGG 1 cut(s) 333
AflII CTTAAG 1 cut(s) 956
AgsI TTSAA 8 cut(s) 376, 399, 450, 841, 1075, 1109, 1156, 1201
AhdI GACNNNNNGTC 1 cut(s) 151
AhlI ACTAGT 1 cut(s) 782
AjnI CCWGG 2 cut(s) 332, 1393
AjuI GAANNNNNNNTTGG 2 cut(s) 1452, 1484
AloI GAACNNNNNNTCC 2 cut(s) 1160, 1192
AluBI AGCT 9 cut(s) 81, 135, 190, 266, 493, 1007, 1360, 1428, 1519
AluI AGCT 9 cut(s) 81, 135, 190, 266, 493, 1007, 1360, 1428, 1519
Alw21I GWGCWC 4 cut(s) 192, 244, 1009, 1521
Alw26I GTCTC 4 cut(s) 500, 563, 662, 1082
AlwI GGATC 1 cut(s) 64
Ama87I CYCGRG 2 cut(s) 362, 1498
AoxI GGCC 3 cut(s) 86, 1560, 1584
ApaI GGGCCC 1 cut(s) 1564
ApeKI GCWGC 6 cut(s) 13, 132, 135, 205, 263, 337
ApoI RAATTY 1 cut(s) 1398
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
Asp700I GAANNNNTTC 1 cut(s) 394
AspS9I GGNCC 4 cut(s) 86, 297, 1560, 1561
AsuHPI GGTGA 2 cut(s) 1349, 1559
AsuII TTCGAA 1 cut(s) 282
AvaI CYCGRG 2 cut(s) 362, 1498
AvaII GGWCC 1 cut(s) 297
BaeGI GKGCMC 1 cut(s) 1564
BalI TGGCCA 1 cut(s) 1586
BanII GRGCYC 4 cut(s) 192, 1009, 1521, 1564
BauI CACGAG 2 cut(s) 1052, 1144
Bbv12I GWGCWC 4 cut(s) 192, 244, 1009, 1521
BbvI GCAGC 5 cut(s) 122, 144, 192, 275, 324
BccI CCATC 6 cut(s) 56, 119, 233, 1159, 1201, 1366
BceAI ACGGC 1 cut(s) 1063
BcgI CGANNNNNNTGC 2 cut(s) 262, 296
BciT130I CCWGG 2 cut(s) 334, 1395
BciVI GTATCC 1 cut(s) 666
BclI TGATCA 1 cut(s) 1314
BcoDI GTCTC 4 cut(s) 500, 563, 662, 1082
BcuI ACTAGT 1 cut(s) 782
BfaI CTAG 4 cut(s) 209, 783, 914, 1185
BfrI CTTAAG 1 cut(s) 956
BfuI GTATCC 1 cut(s) 666
BisI GCNGC 8 cut(s) 14, 89, 133, 136, 206, 264, 338, 444
BlpI GCTNAGC 1 cut(s) 489
BlsI GCNGC 8 cut(s) 15, 90, 134, 137, 207, 265, 339, 445
Bme1390I CCNGG 2 cut(s) 334, 1395
Bme18I GGWCC 1 cut(s) 297
BmeRI GACNNNNNGTC 1 cut(s) 151
BmeT110I CYCGRG 2 cut(s) 362, 1498
BmgT120I GGNCC 4 cut(s) 86, 297, 1560, 1561
BmiI GGNNCC 2 cut(s) 222, 1562
BmrFI CCNGG 2 cut(s) 334, 1395
BmrI ACTGGG 1 cut(s) 268
BmsI GCATC 7 cut(s) 220, 337, 679, 746, 1047, 1053, 1225
BmuI ACTGGG 1 cut(s) 268
BplI GAGNNNNNCTC 2 cut(s) 345, 377
BpmI CTGGAG 1 cut(s) 1251
Bpu10I CCTNAGC 2 cut(s) 82, 903
Bpu1102I GCTNAGC 1 cut(s) 489
Bpu14I TTCGAA 1 cut(s) 282
Bsa29I ATCGAT 1 cut(s) 228
BsaAI YACGTR 1 cut(s) 1302
BsaBI GATNNNNATC 1 cut(s) 677
BsaHI GRCGYC 1 cut(s) 99
BsaJI CCNNGG 1 cut(s) 768
BsaWI WCCGGW 2 cut(s) 603, 1447
BsaXI ACNNNNNCTCC 6 cut(s) 177, 207, 1160, 1190, 1367, 1397
Bsc4I CCNNNNNNNGG 1 cut(s) 333
Bse1I ACTGG 3 cut(s) 263, 514, 1234
Bse3DI GCAATG 2 cut(s) 356, 575
Bse8I GATNNNNATC 1 cut(s) 677
BseBI CCWGG 2 cut(s) 334, 1395
BseCI ATCGAT 1 cut(s) 228
BseDI CCNNGG 1 cut(s) 768
BseGI GGATG 5 cut(s) 111, 352, 1118, 1179, 1412
BseJI GATNNNNATC 1 cut(s) 677
BseLI CCNNNNNNNGG 1 cut(s) 333
BseMI GCAATG 2 cut(s) 356, 575
BseMII CTCAG 2 cut(s) 96, 485
BseNI ACTGG 3 cut(s) 263, 514, 1234
BseRI GAGGAG 1 cut(s) 821
BseSI GKGCMC 1 cut(s) 1564
BseXI GCAGC 5 cut(s) 122, 144, 192, 275, 324
BsgI GTGCAG 1 cut(s) 497
Bsh1236I CGCG 2 cut(s) 117, 443
BshFI GGCC 3 cut(s) 88, 1562, 1586
BshVI ATCGAT 1 cut(s) 228
BsiHKAI GWGCWC 4 cut(s) 192, 244, 1009, 1521
BsiHKCI CYCGRG 2 cut(s) 362, 1498
BsiSI CCGG 3 cut(s) 604, 1125, 1448
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 1 cut(s) 333
BsmAI GTCTC 4 cut(s) 500, 563, 662, 1082
BsmBI CGTCTC 1 cut(s) 662
BsmFI GGGAC 1 cut(s) 158
BsnI GGCC 3 cut(s) 88, 1562, 1586
BsoBI CYCGRG 2 cut(s) 362, 1498
Bsp119I TTCGAA 1 cut(s) 282
Bsp120I GGGCCC 1 cut(s) 1560
Bsp1286I GDGCHC 5 cut(s) 192, 244, 1009, 1521, 1564
Bsp1407I TGTACA 1 cut(s) 1508
Bsp143I GATC 3 cut(s) 69, 639, 1314
Bsp1720I GCTNAGC 1 cut(s) 489
BspACI CCGC 5 cut(s) 89, 115, 443, 930, 1173
BspANI GGCC 3 cut(s) 88, 1562, 1586
BspCNI CTCAG 2 cut(s) 95, 486
BspDI ATCGAT 1 cut(s) 228
BspFNI CGCG 2 cut(s) 117, 443
BspHI TCATGA 1 cut(s) 1476
BspLI GGNNCC 2 cut(s) 222, 1562
BspPI GGATC 1 cut(s) 64
BspT104I TTCGAA 1 cut(s) 282
BspTI CTTAAG 1 cut(s) 956
BsrDI GCAATG 2 cut(s) 356, 575
BsrGI TGTACA 1 cut(s) 1508
BsrI ACTGG 3 cut(s) 263, 514, 1234
BssECI CCNNGG 1 cut(s) 768
BssMI GATC 3 cut(s) 69, 639, 1314
BssNI GRCGYC 1 cut(s) 99
BssSI CACGAG 2 cut(s) 1052, 1144
Bst2BI CACGAG 2 cut(s) 1052, 1144
Bst2UI CCWGG 2 cut(s) 334, 1395
Bst4CI ACNGT 1 cut(s) 895
Bst6I CTCTTC 3 cut(s) 660, 746, 1123
BstACI GRCGYC 1 cut(s) 99
BstAFI CTTAAG 1 cut(s) 956
BstAPI GCANNNNNTGC 2 cut(s) 1057, 1244
BstAUI TGTACA 1 cut(s) 1508
BstBAI YACGTR 1 cut(s) 1302
BstBI TTCGAA 1 cut(s) 282
BstC8I GCNNGC 4 cut(s) 480, 761, 1141, 1245
BstDEI CTNAG 5 cut(s) 82, 425, 489, 494, 903
BstDSI CCRYGG 1 cut(s) 768
BstF5I GGATG 5 cut(s) 111, 352, 1118, 1179, 1412
BstFNI CGCG 2 cut(s) 117, 443
BstKTI GATC 3 cut(s) 72, 642, 1317
BstMAI GTCTC 4 cut(s) 500, 563, 662, 1082
BstMBI GATC 3 cut(s) 69, 639, 1314
BstMWI GCNNNNNNNGC 3 cut(s) 239, 1057, 1244
BstNI CCWGG 2 cut(s) 334, 1395
BstNSI RCATGY 2 cut(s) 733, 1247
BstSCI CCNGG 2 cut(s) 332, 1393
BstSLI GKGCMC 1 cut(s) 1564
BstSNI TACGTA 1 cut(s) 1302
BstUI CGCG 2 cut(s) 117, 443
BstV1I GCAGC 5 cut(s) 122, 144, 192, 275, 324
BstXI CCANNNNNNTGG 1 cut(s) 1537
Bsu15I ATCGAT 1 cut(s) 228
BsuI GTATCC 1 cut(s) 666
BsuRI GGCC 3 cut(s) 88, 1562, 1586
BsuTUI ATCGAT 1 cut(s) 228
BtgI CCRYGG 1 cut(s) 768
BtsCI GGATG 5 cut(s) 111, 352, 1118, 1179, 1412
BtsIMutI CAGTG 1 cut(s) 256
Cac8I GCNNGC 4 cut(s) 480, 761, 1141, 1245
CciI TCATGA 1 cut(s) 1476
Cfr13I GGNCC 4 cut(s) 86, 297, 1560, 1561
ClaI ATCGAT 1 cut(s) 228
CseI GACGC 1 cut(s) 5
Csp6I GTAC 4 cut(s) 990, 1286, 1303, 1509
CviAII CATG 7 cut(s) 287, 343, 730, 746, 1048, 1244, 1477
CviQI GTAC 4 cut(s) 990, 1286, 1303, 1509
DdeI CTNAG 5 cut(s) 82, 425, 489, 494, 903
DpnI GATC 3 cut(s) 71, 641, 1316
DpnII GATC 3 cut(s) 69, 639, 1314
DriI GACNNNNNGTC 1 cut(s) 151
EaeI YGGCCR 1 cut(s) 1584
Eam1104I CTCTTC 3 cut(s) 660, 746, 1123
Eam1105I GACNNNNNGTC 1 cut(s) 151
EarI CTCTTC 3 cut(s) 660, 746, 1123
Ecl136II GAGCTC 3 cut(s) 190, 1007, 1519
Eco105I TACGTA 1 cut(s) 1302
Eco24I GRGCYC 4 cut(s) 192, 1009, 1521, 1564
Eco47I GGWCC 1 cut(s) 297
Eco53kI GAGCTC 3 cut(s) 190, 1007, 1519
Eco57I CTGAAG 1 cut(s) 236
Eco88I CYCGRG 2 cut(s) 362, 1498
EcoICRI GAGCTC 3 cut(s) 190, 1007, 1519
EcoO109I RGGNCCY 2 cut(s) 1560, 1561
EcoRII CCWGG 2 cut(s) 332, 1393
EcoT22I ATGCAT 1 cut(s) 1046
EcoT38I GRGCYC 4 cut(s) 192, 1009, 1521, 1564
Esp3I CGTCTC 1 cut(s) 662
FaeI CATG 7 cut(s) 290, 346, 733, 749, 1051, 1247, 1480
FalI AAGNNNNNCTT 2 cut(s) 939, 971
FaqI GGGAC 1 cut(s) 158
FatI CATG 7 cut(s) 286, 342, 729, 745, 1047, 1243, 1476
FauI CCCGC 1 cut(s) 1166
FauNDI CATATG 2 cut(s) 636, 1513
FbaI TGATCA 1 cut(s) 1314
Fnu4HI GCNGC 8 cut(s) 14, 89, 133, 136, 206, 264, 338, 444
FokI GGATG 5 cut(s) 98, 359, 1125, 1166, 1399
FriOI GRGCYC 4 cut(s) 192, 1009, 1521, 1564
Fsp4HI GCNGC 8 cut(s) 14, 89, 133, 136, 206, 264, 338, 444
FspBI CTAG 4 cut(s) 209, 783, 914, 1185
GluI GCNGC 8 cut(s) 14, 89, 133, 136, 206, 264, 338, 444
GsuI CTGGAG 1 cut(s) 1251
HaeIII GGCC 3 cut(s) 88, 1562, 1586
HapII CCGG 3 cut(s) 604, 1125, 1448
HgaI GACGC 1 cut(s) 5
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 7 cut(s) 290, 346, 733, 749, 1051, 1247, 1480
HincII GTYRAC 2 cut(s) 727, 1260
HindII GTYRAC 2 cut(s) 727, 1260
HinfI GANTC 8 cut(s) 119, 167, 365, 416, 556, 742, 1376, 1473
HpaI GTTAAC 1 cut(s) 1260
HpaII CCGG 3 cut(s) 604, 1125, 1448
HphI GGTGA 2 cut(s) 1349, 1559
Hpy166II GTNNAC 4 cut(s) 156, 727, 925, 1260
Hpy188I TCNGA 6 cut(s) 57, 128, 601, 660, 1314, 1575
Hpy188III TCNNGA 6 cut(s) 196, 796, 869, 1346, 1477, 1522
Hpy8I GTNNAC 4 cut(s) 156, 727, 925, 1260
Hpy99I CGWCG 5 cut(s) 21, 104, 197, 593, 1295
HpyCH4III ACNGT 1 cut(s) 895
HpyCH4IV ACGT 4 cut(s) 99, 238, 541, 1301
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 1057, 1244
HpyF3I CTNAG 5 cut(s) 82, 425, 489, 494, 903
HpySE526I ACGT 4 cut(s) 99, 238, 541, 1301
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 7 cut(s) 290, 346, 733, 749, 1051, 1247, 1480
Ksp22I TGATCA 1 cut(s) 1314
KspAI GTTAAC 1 cut(s) 1260
Kzo9I GATC 3 cut(s) 69, 639, 1314
LmnI GCTCC 2 cut(s) 1004, 1524
Lsp1109I GCAGC 5 cut(s) 122, 144, 192, 275, 324
LweI GCATC 7 cut(s) 220, 337, 679, 746, 1047, 1053, 1225
MaeI CTAG 4 cut(s) 209, 783, 914, 1185
MaeII ACGT 4 cut(s) 99, 238, 541, 1301
MaeIII GTNAC 4 cut(s) 431, 566, 1148, 1337
MalI GATC 3 cut(s) 71, 641, 1316
MboI GATC 3 cut(s) 69, 639, 1314
MfeI CAATTG 2 cut(s) 139, 876
MhlI GDGCHC 5 cut(s) 192, 244, 1009, 1521, 1564
MlsI TGGCCA 1 cut(s) 1586
MluNI TGGCCA 1 cut(s) 1586
MlyI GAGTC 5 cut(s) 113, 176, 374, 565, 1385
MmeI TCCRAC 2 cut(s) 367, 1192
MnlI CCTC 9 cut(s) 430, 708, 799, 802, 931, 956, 1013, 1374, 1444
Mox20I TGGCCA 1 cut(s) 1586
Mph1103I ATGCAT 1 cut(s) 1046
MroXI GAANNNNTTC 1 cut(s) 394
MscI TGGCCA 1 cut(s) 1586
MseI TTAA 7 cut(s) 35, 174, 675, 909, 957, 1259, 1597
MslI CAYNNNNRTG 1 cut(s) 1248
Msp20I TGGCCA 1 cut(s) 1586
MspA1I CMGCKG 1 cut(s) 135
MspCI CTTAAG 1 cut(s) 956
MspI CCGG 3 cut(s) 604, 1125, 1448
MspR9I CCNGG 2 cut(s) 334, 1395
MunI CAATTG 2 cut(s) 139, 876
MvaI CCWGG 2 cut(s) 334, 1395
MvnI CGCG 2 cut(s) 117, 443
MwoI GCNNNNNNNGC 3 cut(s) 239, 1057, 1244
NdeI CATATG 2 cut(s) 636, 1513
NdeII GATC 3 cut(s) 69, 639, 1314
NlaIII CATG 7 cut(s) 290, 346, 733, 749, 1051, 1247, 1480
NlaIV GGNNCC 2 cut(s) 222, 1562
NmuCI GTSAC 1 cut(s) 1337
NsiI ATGCAT 1 cut(s) 1046
NspI RCATGY 2 cut(s) 733, 1247
NspV TTCGAA 1 cut(s) 282
PaeI GCATGC 1 cut(s) 1247
PaeR7I CTCGAG 1 cut(s) 362
PagI TCATGA 1 cut(s) 1476
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 1496
PdmI GAANNNNTTC 1 cut(s) 394
PfeI GAWTC 3 cut(s) 416, 742, 1473
PflFI GACNNNGTC 1 cut(s) 101
PfoI TCCNGGA 1 cut(s) 1393
PkrI GCNGC 8 cut(s) 15, 90, 134, 137, 207, 265, 339, 445
PleI GAGTC 5 cut(s) 113, 175, 373, 564, 1384
PpsI GAGTC 5 cut(s) 113, 175, 373, 564, 1384
Ppu21I YACGTR 1 cut(s) 1302
Psp124BI GAGCTC 3 cut(s) 192, 1009, 1521
Psp6I CCWGG 2 cut(s) 332, 1393
PspGI CCWGG 2 cut(s) 332, 1393
PspN4I GGNNCC 2 cut(s) 222, 1562
PspOMI GGGCCC 1 cut(s) 1560
PspPI GGNCC 4 cut(s) 86, 297, 1560, 1561
PspXI VCTCGAGB 1 cut(s) 362
PsyI GACNNNGTC 1 cut(s) 101
PvuII CAGCTG 1 cut(s) 135
RsaI GTAC 4 cut(s) 991, 1287, 1304, 1510
RsaNI GTAC 4 cut(s) 990, 1286, 1303, 1509
RseI CAYNNNNRTG 1 cut(s) 1248
SacI GAGCTC 3 cut(s) 192, 1009, 1521
SaqAI TTAA 7 cut(s) 35, 174, 675, 909, 957, 1259, 1597
SatI GCNGC 8 cut(s) 14, 89, 133, 136, 206, 264, 338, 444
Sau3AI GATC 3 cut(s) 69, 639, 1314
Sau96I GGNCC 4 cut(s) 86, 297, 1560, 1561
SchI GAGTC 5 cut(s) 113, 176, 374, 565, 1385
ScrFI CCNGG 2 cut(s) 334, 1395
SduI GDGCHC 5 cut(s) 192, 244, 1009, 1521, 1564
SfaNI GCATC 7 cut(s) 220, 337, 679, 746, 1047, 1053, 1225
Sfr274I CTCGAG 1 cut(s) 362
SfuI TTCGAA 1 cut(s) 282
SinI GGWCC 1 cut(s) 297
SlaI CTCGAG 1 cut(s) 362
SmiMI CAYNNNNRTG 1 cut(s) 1248
SmlI CTYRAG 2 cut(s) 362, 956
SmoI CTYRAG 2 cut(s) 362, 956
SnaBI TACGTA 1 cut(s) 1302
SpeI ACTAGT 1 cut(s) 782
SphI GCATGC 1 cut(s) 1247
SsiI CCGC 5 cut(s) 89, 115, 443, 930, 1173
SspMI CTAG 4 cut(s) 209, 783, 914, 1185
SstI GAGCTC 3 cut(s) 192, 1009, 1521
StyD4I CCNGG 2 cut(s) 332, 1393
TaaI ACNGT 1 cut(s) 895
TaiI ACGT 4 cut(s) 102, 241, 544, 1304
TatI WGTACW 1 cut(s) 1508
TauI GCSGC 2 cut(s) 91, 446
TfiI GAWTC 3 cut(s) 416, 742, 1473
Tru1I TTAA 7 cut(s) 35, 174, 675, 909, 957, 1259, 1597
Tru9I TTAA 7 cut(s) 35, 174, 675, 909, 957, 1259, 1597
TscAI CASTG 1 cut(s) 263
TseFI GTSAC 1 cut(s) 1337
TseI GCWGC 6 cut(s) 13, 132, 135, 205, 263, 337
Tsp45I GTSAC 1 cut(s) 1337
TspDTI ATGAA 8 cut(s) 305, 734, 960, 973, 1168, 1266, 1465, 1493
TspGWI ACGGA 4 cut(s) 160, 198, 444, 757
TspRI CASTG 1 cut(s) 263
Tth111I GACNNNGTC 1 cut(s) 101
Vha464I CTTAAG 1 cut(s) 956
VpaK11BI GGWCC 1 cut(s) 297
XapI RAATTY 1 cut(s) 1398
XceI RCATGY 2 cut(s) 733, 1247
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XhoI CTCGAG 1 cut(s) 362
XmnI GAANNNNTTC 1 cut(s) 394
XspI CTAG 4 cut(s) 209, 783, 914, 1185
ZraI GACGTC 1 cut(s) 100
Zsp2I ATGCAT 1 cut(s) 1046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.