Rw7G034670

Pentatricopeptide repeat-containing protein At1g66345

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011738
Physical Location & Seq
Forward (+)
51494417 .. 51496858
2442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G034670.1

Sequence Viewer

Length: 1602 bp
ATGGGTTTTTTCCTGCGTCGAATTACACATTCTTTAATTTTCTTGAAACCCATCAATCACAGTACTCAACTGATCCACACACAATCAGCACCAAACAATGTTGTCAGAGCCATCTGTGATTCTTTCAGAACCGGTTGGAACTGGGATGCTCTGACATCAAGATTTGATTCTCTCAAATTAGATGGAAAGCTTGTTGAGAGTGTGTTGTTAGAACTGAAGGAACCAGTTGATGCAAAACGTGCTCTGGGTTTCTTCCACTGGGCATCAAACAGGAAAGGGTTTGATCATGAGGTCTGGTCCTACTCCATAGCCATTCATATTTTGGTCAGAGCCAAGATGGTCATGGATGCTAGAGCTTTGATCGAATCGGTTTTGAAGAAGAATGTTGGAAATGAGTTGAAGTTTTCGGTGGTGGATTCGTTACTTAGTAGTTACGAGGTCACTGCTTCGAACCCTTTTGTGTTTGATTTGCTTGTACAGGCTTATGCTAAAATGAGAATGTTTGAGACTGGTTTTGATGTTTGTTGCTATTTGAGGGATCGTGGGTTGCCTTTAAGTCTTACAAGTTACAATAGTTTGCTTCGAGTTGTTCAAAGATCGGACCGAAATGGTCTGGTTTGGAAGATTTATGAGCATATTGTGGAAAGGAGGAGTTATCCGAATGATGAGACGGTGAGGATCTTGATTGATGCTTTGTGCAAGGAAGGGGAGTTGCGGAAATGTGCTGACATGTTGGAGAGGATTCATGGGAAGAAATGTTCTCCTTCTGTGATTGTGAATGCTAGTTTGGTTTTTAGGATTTTGGAGGAGGGTAGAGTTGAAGAAGGTGTGGTATTGTTGAAGAGAATGTTGCAGAAGAATATGGTTCTTGATACGATTGCTTACTCATTGATTGTTCATGCTAAAGTTAAACTAGGAAATTTAAGCTCTGCACAGCAGGTGTATGAAGAAATGCTTAAGAGAGGTTTTCGAGCAAACTCTTTTGTTTATACCTTGTTCATTGAGGCTTATTGTAAAGCTGAAAGAATTGATGAAGCACAATCCATGATGAATGAGATGGGAAATATGGATTTGAAGCCATATGCTGAGAGCTATAATTTTCTTATTGAGGGATGTGCTAGAGCTGGTAGAGTGGAAGAAAGTGTGAGTTACTTGAAGCAGATGGTGGAGAGCAGGTTCATTCCTAGTCTTGGGGCTTTCAATGAGATGGTTGGAAAGCTATGCGAAATTGGGGATGTGGACCAAGCCAATGTGATGTTAACGATACTGTTAGATAAAGGGTTCTTACCGAATGACATTACATACTCTCTTCTGATTGACGGGTATGCGAGAAAAGGGGAGAATGATGAAGTTCTGAAGCTCTATTATGAGATGGAGTCCAGGTCACTTCCTCCTGGAATATTGGTTTTTACATCATTGATAAAAAGCTTTTGCCAATGCGGGAAATTGGAGGAAGCAAAAAAGTATTTCAGAATCATGAAAGATCGATCAATAGCCCCAAGTTTATCTACATATGAGACATTGATTGCGAACCACTTTGAGCAGGGAAATACAGAGAAGGCTGATTGCTTGCCTTGCAATGAAATGGTTTTACAGGACTGA

Protein Analysis

533

Amino Acids

60.85

Weight (kDa)

6.81

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 291 - 339 3.1e-08 PPR repeat family
PPR_1 PF12854 321 - 353 8.3e-07 PPR repeat
PPR_2 PF13041 326 - 374 3.6e-09 PPR repeat family
PPR PF01535 364 - 392 6.2e-06 PPR repeat
PPR_2 PF13041 396 - 444 2.6e-07 PPR repeat family
PPR_1 PF12854 426 - 459 5.4e-06 PPR repeat
PPR PF01535 433 - 461 1.2e-06 PPR repeat
PPR_2 PF13041 438 - 478 6.2e-07 PPR repeat family
PPR_3 PF13812 454 - 509 6.4e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 463 - 494 5e-06 PPR repeat
PPR_2 PF13041 465 - 509 1.4e-09 PPR repeat family
PPR PF01535 469 - 497 1.1e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 928
Acc36I ACCTGC 2 cut(s) 928, 1164
AciI CCGC 2 cut(s) 715, 1440
AclWI GGATC 3 cut(s) 67, 546, 686
AcsI RAATTY 1 cut(s) 919
AcuI CTGAAG 2 cut(s) 236, 1376
AfaI GTAC 2 cut(s) 64, 477
AfiI CCNNNNNNNGG 1 cut(s) 1190
AflII CTTAAG 1 cut(s) 956
AflIII ACRYGT 1 cut(s) 729
AgeI ACCGGT 1 cut(s) 131
AgsI TTSAA 9 cut(s) 46, 376, 400, 593, 821, 841, 1075, 1156, 1201
AjnI CCWGG 2 cut(s) 1379, 1393
AjuI GAANNNNNNNTTGG 2 cut(s) 770, 802
AloI GAACNNNNNNTCC 4 cut(s) 742, 774, 1160, 1192
AluBI AGCT 9 cut(s) 190, 356, 927, 1019, 1092, 1124, 1219, 1360, 1428
AluI AGCT 9 cut(s) 190, 356, 927, 1019, 1092, 1124, 1219, 1360, 1428
Alw21I GWGCWC 1 cut(s) 244
Alw26I GTCTC 3 cut(s) 500, 662, 1511
AlwI GGATC 3 cut(s) 67, 546, 686
ApoI RAATTY 1 cut(s) 919
AsiGI ACCGGT 1 cut(s) 131
AspS9I GGNCC 3 cut(s) 297, 601, 1240
AsuHPI GGTGA 1 cut(s) 685
AsuII TTCGAA 1 cut(s) 449
AvaII GGWCC 3 cut(s) 297, 601, 1240
Bbv12I GWGCWC 1 cut(s) 244
BccI CCATC 8 cut(s) 59, 119, 176, 331, 1051, 1156, 1201, 1366
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BciT130I CCWGG 2 cut(s) 1381, 1395
BclI TGATCA 1 cut(s) 283
BcoDI GTCTC 3 cut(s) 500, 662, 1511
BfaI CTAG 5 cut(s) 351, 783, 914, 1119, 1185
BfrI CTTAAG 1 cut(s) 956
BfuAI ACCTGC 2 cut(s) 928, 1164
BmcAI AGTACT 1 cut(s) 64
Bme1390I CCNGG 2 cut(s) 1381, 1395
Bme18I GGWCC 3 cut(s) 297, 601, 1240
BmgT120I GGNCC 3 cut(s) 297, 601, 1240
BmiI GGNNCC 1 cut(s) 222
BmrFI CCNGG 2 cut(s) 1381, 1395
BmrI ACTGGG 2 cut(s) 151, 268
BmsI GCATC 5 cut(s) 136, 220, 272, 337, 679
BmuI ACTGGG 2 cut(s) 151, 268
Bpu14I TTCGAA 1 cut(s) 449
Bsa29I ATCGAT 1 cut(s) 1486
BsaWI WCCGGW 1 cut(s) 131
BsaXI ACNNNNNCTCC 4 cut(s) 1160, 1190, 1367, 1397
Bsc4I CCNNNNNNNGG 1 cut(s) 1190
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 4 cut(s) 146, 224, 263, 514
Bse3DI GCAATG 1 cut(s) 1585
BseBI CCWGG 2 cut(s) 1381, 1395
BseCI ATCGAT 1 cut(s) 1486
BseGI GGATG 4 cut(s) 151, 352, 1118, 1240
BseLI CCNNNNNNNGG 1 cut(s) 1190
BseMI GCAATG 1 cut(s) 1585
BseMII CTCAG 1 cut(s) 1077
BseNI ACTGG 4 cut(s) 146, 224, 263, 514
BseRI GAGGAG 2 cut(s) 664, 821
BsgI GTGCAG 1 cut(s) 915
BshTI ACCGGT 1 cut(s) 131
BshVI ATCGAT 1 cut(s) 1486
BsiHKAI GWGCWC 1 cut(s) 244
BsiSI CCGG 1 cut(s) 132
BslI CCNNNNNNNGG 1 cut(s) 1190
BsmAI GTCTC 3 cut(s) 500, 662, 1511
BsmBI CGTCTC 1 cut(s) 662
BsmI GAATGC 1 cut(s) 784
Bsp119I TTCGAA 1 cut(s) 449
Bsp1286I GDGCHC 1 cut(s) 244
Bsp1407I TGTACA 1 cut(s) 475
Bsp143I GATC 8 cut(s) 72, 283, 360, 538, 596, 678, 1483, 1487
BspACI CCGC 2 cut(s) 715, 1440
BspCNI CTCAG 1 cut(s) 1078
BspDI ATCGAT 1 cut(s) 1486
BspHI TCATGA 2 cut(s) 286, 1476
BspLI GGNNCC 1 cut(s) 222
BspMI ACCTGC 2 cut(s) 928, 1164
BspPI GGATC 3 cut(s) 67, 546, 686
BspT104I TTCGAA 1 cut(s) 449
BspTI CTTAAG 1 cut(s) 956
BsrDI GCAATG 1 cut(s) 1585
BsrFI RCCGGY 1 cut(s) 131
BsrGI TGTACA 1 cut(s) 475
BsrI ACTGG 4 cut(s) 146, 224, 263, 514
BssAI RCCGGY 1 cut(s) 131
BssMI GATC 8 cut(s) 72, 283, 360, 538, 596, 678, 1483, 1487
Bst2UI CCWGG 2 cut(s) 1381, 1395
Bst4CI ACNGT 3 cut(s) 62, 673, 1269
Bst6I CTCTTC 2 cut(s) 836, 1314
BstAFI CTTAAG 1 cut(s) 956
BstAPI GCANNNNNTGC 1 cut(s) 239
BstAUI TGTACA 1 cut(s) 475
BstBI TTCGAA 1 cut(s) 449
BstC8I GCNNGC 1 cut(s) 1571
BstDEI CTNAG 2 cut(s) 425, 1086
BstF5I GGATG 4 cut(s) 151, 352, 1118, 1240
BstKTI GATC 8 cut(s) 75, 286, 363, 541, 599, 681, 1486, 1490
BstMAI GTCTC 3 cut(s) 500, 662, 1511
BstMBI GATC 8 cut(s) 72, 283, 360, 538, 596, 678, 1483, 1487
BstMWI GCNNNNNNNGC 2 cut(s) 239, 1575
BstNI CCWGG 2 cut(s) 1381, 1395
BstNSI RCATGY 1 cut(s) 733
BstSCI CCNGG 2 cut(s) 1379, 1393
BstX2I RGATCY 1 cut(s) 678
BstYI RGATCY 1 cut(s) 678
Bsu15I ATCGAT 1 cut(s) 1486
BsuTUI ATCGAT 1 cut(s) 1486
BtsCI GGATG 4 cut(s) 151, 352, 1118, 1240
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 2 cut(s) 256, 441
BveI ACCTGC 2 cut(s) 928, 1164
Cac8I GCNNGC 1 cut(s) 1571
CciI TCATGA 2 cut(s) 286, 1476
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 3 cut(s) 297, 601, 1240
ClaI ATCGAT 1 cut(s) 1486
CpoI CGGWCCG 1 cut(s) 601
CseI GACGC 1 cut(s) 5
Csp6I GTAC 2 cut(s) 63, 476
CspAI ACCGGT 1 cut(s) 131
CspI CGGWCCG 1 cut(s) 601
CviAII CATG 7 cut(s) 287, 343, 730, 746, 899, 1045, 1477
CviQI GTAC 2 cut(s) 63, 476
DdeI CTNAG 2 cut(s) 425, 1086
DpnI GATC 8 cut(s) 74, 285, 362, 540, 598, 680, 1485, 1489
DpnII GATC 8 cut(s) 72, 283, 360, 538, 596, 678, 1483, 1487
Eam1104I CTCTTC 2 cut(s) 836, 1314
EarI CTCTTC 2 cut(s) 836, 1314
Eco47I GGWCC 3 cut(s) 297, 601, 1240
Eco57I CTGAAG 2 cut(s) 236, 1376
EcoRII CCWGG 2 cut(s) 1379, 1393
Esp3I CGTCTC 1 cut(s) 662
FaeI CATG 7 cut(s) 290, 346, 733, 749, 902, 1048, 1480
FalI AAGNNNNNCTT 2 cut(s) 939, 971
FatI CATG 7 cut(s) 286, 342, 729, 745, 898, 1044, 1476
FauI CCCGC 1 cut(s) 1433
FauNDI CATATG 2 cut(s) 1081, 1513
FbaI TGATCA 1 cut(s) 283
FokI GGATG 4 cut(s) 158, 359, 1125, 1247
FspBI CTAG 5 cut(s) 351, 783, 914, 1119, 1185
HapII CCGG 1 cut(s) 132
HgaI GACGC 1 cut(s) 5
Hin1II CATG 7 cut(s) 290, 346, 733, 749, 902, 1048, 1480
HincII GTYRAC 1 cut(s) 1260
HindII GTYRAC 1 cut(s) 1260
HindIII AAGCTT 2 cut(s) 188, 1426
HinfI GANTC 7 cut(s) 119, 167, 365, 416, 742, 1376, 1473
HpaI GTTAAC 1 cut(s) 1260
HpaII CCGG 1 cut(s) 132
HphI GGTGA 1 cut(s) 685
Hpy166II GTNNAC 2 cut(s) 1240, 1260
Hpy188I TCNGA 9 cut(s) 107, 128, 153, 329, 601, 660, 1314, 1356, 1472
Hpy188III TCNNGA 6 cut(s) 43, 159, 287, 682, 869, 1477
Hpy8I GTNNAC 2 cut(s) 1240, 1260
Hpy99I CGWCG 1 cut(s) 21
HpyAV CCTTC 5 cut(s) 211, 698, 774, 818, 1552
HpyCH4III ACNGT 3 cut(s) 62, 673, 1269
HpyCH4IV ACGT 1 cut(s) 238
HpyCH4V TGCA 5 cut(s) 233, 699, 853, 932, 1578
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 1575
HpyF3I CTNAG 2 cut(s) 425, 1086
HpySE526I ACGT 1 cut(s) 238
Hsp92II CATG 7 cut(s) 290, 346, 733, 749, 902, 1048, 1480
Ksp22I TGATCA 1 cut(s) 283
KspAI GTTAAC 1 cut(s) 1260
Kzo9I GATC 8 cut(s) 72, 283, 360, 538, 596, 678, 1483, 1487
LweI GCATC 5 cut(s) 136, 220, 272, 337, 679
MaeI CTAG 5 cut(s) 351, 783, 914, 1119, 1185
MaeII ACGT 1 cut(s) 238
MaeIII GTNAC 6 cut(s) 420, 431, 439, 566, 1148, 1383
MalI GATC 8 cut(s) 74, 285, 362, 540, 598, 680, 1485, 1489
MboI GATC 8 cut(s) 72, 283, 360, 538, 596, 678, 1483, 1487
MflI RGATCY 1 cut(s) 678
MhlI GDGCHC 1 cut(s) 244
MluCI AATT 8 cut(s) 21, 36, 176, 919, 1026, 1096, 1227, 1445
MlyI GAGTC 1 cut(s) 1385
MmeI TCCRAC 4 cut(s) 116, 367, 714, 1192
MseI TTAA 6 cut(s) 35, 554, 909, 923, 957, 1259
MspCI CTTAAG 1 cut(s) 956
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 2 cut(s) 1381, 1395
Mva1269I GAATGC 1 cut(s) 784
MvaI CCWGG 2 cut(s) 1381, 1395
MwoI GCNNNNNNNGC 2 cut(s) 239, 1575
NdeI CATATG 2 cut(s) 1081, 1513
NdeII GATC 8 cut(s) 72, 283, 360, 538, 596, 678, 1483, 1487
NlaIII CATG 7 cut(s) 290, 346, 733, 749, 902, 1048, 1480
NlaIV GGNNCC 1 cut(s) 222
NmuCI GTSAC 2 cut(s) 439, 1383
NspI RCATGY 1 cut(s) 733
NspV TTCGAA 1 cut(s) 449
PagI TCATGA 2 cut(s) 286, 1476
PaqCI CACCTGC 1 cut(s) 928
PciI ACATGT 1 cut(s) 729
PctI GAATGC 1 cut(s) 784
PfeI GAWTC 6 cut(s) 119, 167, 365, 416, 742, 1473
PfoI TCCNGGA 1 cut(s) 1393
PinAI ACCGGT 1 cut(s) 131
PleI GAGTC 1 cut(s) 1384
PpsI GAGTC 1 cut(s) 1384
PscI ACATGT 1 cut(s) 729
Psp6I CCWGG 2 cut(s) 1379, 1393
PspGI CCWGG 2 cut(s) 1379, 1393
PspN4I GGNNCC 1 cut(s) 222
PspPI GGNCC 3 cut(s) 297, 601, 1240
PsuI RGATCY 1 cut(s) 678
RsaI GTAC 2 cut(s) 64, 477
RsaNI GTAC 2 cut(s) 63, 476
Rsr2I CGGWCCG 1 cut(s) 601
RsrII CGGWCCG 1 cut(s) 601
SaqAI TTAA 6 cut(s) 35, 554, 909, 923, 957, 1259
Sau3AI GATC 8 cut(s) 72, 283, 360, 538, 596, 678, 1483, 1487
Sau96I GGNCC 3 cut(s) 297, 601, 1240
ScaI AGTACT 1 cut(s) 64
SchI GAGTC 1 cut(s) 1385
ScrFI CCNGG 2 cut(s) 1381, 1395
SduI GDGCHC 1 cut(s) 244
SfaNI GCATC 5 cut(s) 136, 220, 272, 337, 679
SfuI TTCGAA 1 cut(s) 449
SinI GGWCC 3 cut(s) 297, 601, 1240
SmlI CTYRAG 1 cut(s) 956
SmoI CTYRAG 1 cut(s) 956
Sse9I AATT 8 cut(s) 21, 36, 176, 919, 1026, 1096, 1227, 1445
SsiI CCGC 2 cut(s) 715, 1440
SspI AATATT 1 cut(s) 1401
SspMI CTAG 5 cut(s) 351, 783, 914, 1119, 1185
StyD4I CCNGG 2 cut(s) 1379, 1393
TaaI ACNGT 3 cut(s) 62, 673, 1269
TaiI ACGT 1 cut(s) 241
TaqI TCGA 6 cut(s) 19, 363, 449, 583, 970, 1486
TaqII GACCGA 1 cut(s) 618
TasI AATT 8 cut(s) 21, 36, 176, 919, 1026, 1096, 1227, 1445
TatI WGTACW 2 cut(s) 62, 475
TfiI GAWTC 6 cut(s) 119, 167, 365, 416, 742, 1473
Tru1I TTAA 6 cut(s) 35, 554, 909, 923, 957, 1259
Tru9I TTAA 6 cut(s) 35, 554, 909, 923, 957, 1259
TscAI CASTG 2 cut(s) 263, 448
TseFI GTSAC 2 cut(s) 439, 1383
Tsp45I GTSAC 2 cut(s) 439, 1383
TspRI CASTG 2 cut(s) 263, 448
Vha464I CTTAAG 1 cut(s) 956
VpaK11BI GGWCC 3 cut(s) 297, 601, 1240
XapI RAATTY 1 cut(s) 919
XceI RCATGY 1 cut(s) 733
XcmI CCANNNNNNNNNTGG 2 cut(s) 319, 340
XspI CTAG 5 cut(s) 351, 783, 914, 1119, 1185
ZrmI AGTACT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.