RLG00000001422

Pentatricopeptide repeat-containing protein At1g66345

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011738
Physical Location & Seq
Reverse (-)
15419913 .. 15421514
1602 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001422

Sequence Viewer

Length: 1602 bp
ATGGGTTTTTTGCTGCGTCGAATTACACAATCTTTAATTTCTTTGAAACCCATCAACCACAGAACTCGACTGATCCACACACACTCAGAACCAGACAATGTTGTCAGAGCCATCTCTGATTCTTTCAGAACCGGGTGGAACTGGGATGCTCTGACATCAAGATTTGATTCTCTCAAATTAGATGGAAAGCTTGTTGAGAGTGTGTTGTTAGAACTGAAGGAACCAGTTGATGCAAAACGTGCTCTGGGTTTCTTCCACTGGGCATCAAACAGGAAAGAGTTTGATCATGGGGTCTGGTCCTACTCCATAGCCATTCATATTTTGGTCAGAGCCAAGATGGTGATGGATGCTAGAGCTTTGATCGAATCGGTTTTGAAGAAGAATGTTGGAAATGAGTTGAAGTTTTCGGTTGTGGATTCGTTACTTAGTAGTTACGAGGTTACTGCTTCGAACCCTTTTGTGTTTGATTTGCTTGTACAGGCTTATGCTAAATTGAGAATGTTTGAGACTGGTTTTGATGTTTGTTGCTATTTGAGGGAACGTGGGTTGCCTTTAGGTCTTACAAGTTACAATAGTTTGCTTCGAGTTGTTCAAAGATCGGACCGAAATGGTCTGGTTTGGAAGATTTATGAGCATATGGTGGAAAAAAGAAGTTATCCGAATGATGAGACGGTGAGGATCTTGATTGATGCTTTGTGCAAGGAAGGGGAGTTGCGGAAATATGCCGACATGTTGGAGAGGATTCATGGGAAGAAATGTTCTCCTTCTGTGATTGTGAATGCTAGTTTGGTGTTTAGGATTTTGGAGGAGGGTAGAGTTGAAGTAGGTGTGGTATTGTTGAAGAGAATGTTGCAAAAGAATATGGTTCTTGATACGATTGCTTACTCATTGATTGTTTATGCTAAAGTTAAACTAGGAAATTTAGGCTCTGCACAGGAGGTGTATGAAGAAATGCTTAAGAGAGGTTTTCGAGCAAACTCTTTTGTTTATACCTTGTTCATTGAGGCTCATTGTAAAGCTGGAAGAATTGATGAAGCACAATCCATGATGAATGAGATGGGAAATATGGATTTGAAGCCATATGATGAGAGCTATAATTTTCTTATTGAGGGATGTGTTAGAGCTGGTAGAGTGGAAGAAAGTGTGAGTTACTTGAAGCAGATGATGGAGAGCAGGTTCATTCCTAGTCTTGGGGCTTTCAATGGGATGGTTGGAAAGCTATGTGAAATTGGGGATGCGGACCAAGCCAATGCGATGTTAACTATACTGTTAGATAAAGGGTTCTTACCGAATGACCTTACGTACTCTCTTCTGATTGACGGGTATGCGAGAAAAGGGGAGAATGATGAAGTTCTGAAGCTATATTATGAGATGGAGTCCAGGTCACTTTCTCCTGGAATATTGGTTTTTACATCATTGATAAAGAGCTTTTGCCAATGCGGGAAATTGGAGGAAGCAAAGAAGTATTTCAGAGTCATGAAAGATCGATCAATAGCCCCAAGTTTATCTACATATGAGACATTGATTGCGAACCACTTTGAGCAGGGAAATACAGAAAGGGCTGATTGCTTGCCTTGCAATGAAATGGTTGTACAAGACTGA

Protein Analysis

534

Amino Acids

60.75

Weight (kDa)

7.12

Isoelectric Point (pI)

33.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 189 - 234 4.3e-07 PPR repeat family
PPR_1 PF12854 321 - 353 1.7e-07 PPR repeat
PPR_2 PF13041 326 - 374 4.7e-09 PPR repeat family
PPR_2 PF13041 396 - 444 1.7e-06 PPR repeat family
PPR_2 PF13041 434 - 478 2.6e-08 PPR repeat family
PPR PF01535 434 - 461 2.4e-06 PPR repeat
PPR_3 PF13812 454 - 509 2.2e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 463 - 494 1.1e-06 PPR repeat
PPR_2 PF13041 465 - 509 1.5e-09 PPR repeat family
PPR PF01535 469 - 497 1e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 101
Acc36I ACCTGC 1 cut(s) 1164
AciI CCGC 3 cut(s) 715, 1238, 1440
AclWI GGATC 2 cut(s) 67, 686
AcsI RAATTY 1 cut(s) 919
AcuI CTGAAG 2 cut(s) 236, 1376
AfaI GTAC 3 cut(s) 477, 1304, 1593
AfiI CCNNNNNNNGG 1 cut(s) 1190
AflII CTTAAG 1 cut(s) 956
AflIII ACRYGT 1 cut(s) 729
AgsI TTSAA 9 cut(s) 46, 376, 400, 593, 821, 841, 1075, 1156, 1201
AjnI CCWGG 2 cut(s) 1379, 1393
AjuI GAANNNNNNNTTGG 2 cut(s) 770, 802
AloI GAACNNNNNNTCC 4 cut(s) 742, 774, 1160, 1192
AluBI AGCT 8 cut(s) 190, 356, 1019, 1092, 1124, 1219, 1360, 1428
AluI AGCT 8 cut(s) 190, 356, 1019, 1092, 1124, 1219, 1360, 1428
Alw21I GWGCWC 1 cut(s) 244
Alw26I GTCTC 3 cut(s) 500, 662, 1511
AlwI GGATC 2 cut(s) 67, 686
ApeKI GCWGC 1 cut(s) 13
ApoI RAATTY 1 cut(s) 919
Asp700I GAANNNNTTC 1 cut(s) 1466
AspS9I GGNCC 3 cut(s) 297, 601, 1240
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 2 cut(s) 352, 685
AsuII TTCGAA 1 cut(s) 449
AvaII GGWCC 3 cut(s) 297, 601, 1240
Bbv12I GWGCWC 1 cut(s) 244
BccI CCATC 9 cut(s) 59, 119, 176, 331, 337, 1051, 1159, 1201, 1366
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BciT130I CCWGG 2 cut(s) 1381, 1395
BclI TGATCA 1 cut(s) 283
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 3 cut(s) 500, 662, 1511
BfaI CTAG 4 cut(s) 351, 783, 914, 1185
BfrI CTTAAG 1 cut(s) 956
BfuAI ACCTGC 1 cut(s) 1164
BisI GCNGC 1 cut(s) 14
BlsI GCNGC 1 cut(s) 15
Bme1390I CCNGG 3 cut(s) 133, 1381, 1395
Bme18I GGWCC 3 cut(s) 297, 601, 1240
BmgT120I GGNCC 3 cut(s) 297, 601, 1240
BmiI GGNNCC 1 cut(s) 222
BmrFI CCNGG 3 cut(s) 133, 1381, 1395
BmrI ACTGGG 2 cut(s) 151, 268
BmsI GCATC 6 cut(s) 136, 220, 272, 337, 679, 1225
BmuI ACTGGG 2 cut(s) 151, 268
Bpu14I TTCGAA 1 cut(s) 449
BpuMI CCSGG 1 cut(s) 133
Bsa29I ATCGAT 1 cut(s) 1486
BsaAI YACGTR 1 cut(s) 1302
BsaXI ACNNNNNCTCC 4 cut(s) 1160, 1190, 1367, 1397
Bsc4I CCNNNNNNNGG 1 cut(s) 1190
Bse1I ACTGG 4 cut(s) 146, 224, 263, 514
Bse3DI GCAATG 1 cut(s) 1585
BseBI CCWGG 2 cut(s) 1381, 1395
BseCI ATCGAT 1 cut(s) 1486
BseGI GGATG 5 cut(s) 151, 352, 1118, 1212, 1240
BseLI CCNNNNNNNGG 1 cut(s) 1190
BseMI GCAATG 1 cut(s) 1585
BseMII CTCAG 1 cut(s) 99
BseNI ACTGG 4 cut(s) 146, 224, 263, 514
BseRI GAGGAG 1 cut(s) 821
BsgI GTGCAG 1 cut(s) 915
BshVI ATCGAT 1 cut(s) 1486
BsiHKAI GWGCWC 1 cut(s) 244
BsiSI CCGG 1 cut(s) 132
BslI CCNNNNNNNGG 1 cut(s) 1190
BsmAI GTCTC 3 cut(s) 500, 662, 1511
BsmBI CGTCTC 1 cut(s) 662
BsmI GAATGC 1 cut(s) 784
Bsp119I TTCGAA 1 cut(s) 449
Bsp1286I GDGCHC 1 cut(s) 244
Bsp1407I TGTACA 2 cut(s) 475, 1591
Bsp143I GATC 7 cut(s) 72, 283, 360, 596, 678, 1483, 1487
BspACI CCGC 3 cut(s) 715, 1238, 1440
BspCNI CTCAG 1 cut(s) 98
BspDI ATCGAT 1 cut(s) 1486
BspHI TCATGA 1 cut(s) 1476
BspLI GGNNCC 1 cut(s) 222
BspMI ACCTGC 1 cut(s) 1164
BspPI GGATC 2 cut(s) 67, 686
BspT104I TTCGAA 1 cut(s) 449
BspTI CTTAAG 1 cut(s) 956
BsrDI GCAATG 1 cut(s) 1585
BsrGI TGTACA 2 cut(s) 475, 1591
BsrI ACTGG 4 cut(s) 146, 224, 263, 514
BssMI GATC 7 cut(s) 72, 283, 360, 596, 678, 1483, 1487
Bst2UI CCWGG 2 cut(s) 1381, 1395
Bst4CI ACNGT 2 cut(s) 673, 1269
Bst6I CTCTTC 2 cut(s) 836, 1314
BstAFI CTTAAG 1 cut(s) 956
BstAPI GCANNNNNTGC 1 cut(s) 239
BstAUI TGTACA 2 cut(s) 475, 1591
BstBAI YACGTR 1 cut(s) 1302
BstBI TTCGAA 1 cut(s) 449
BstC8I GCNNGC 1 cut(s) 1571
BstDEI CTNAG 2 cut(s) 85, 425
BstF5I GGATG 5 cut(s) 151, 352, 1118, 1212, 1240
BstKTI GATC 7 cut(s) 75, 286, 363, 599, 681, 1486, 1490
BstMAI GTCTC 3 cut(s) 500, 662, 1511
BstMBI GATC 7 cut(s) 72, 283, 360, 596, 678, 1483, 1487
BstMWI GCNNNNNNNGC 3 cut(s) 239, 1244, 1575
BstNI CCWGG 2 cut(s) 1381, 1395
BstNSI RCATGY 1 cut(s) 733
BstSCI CCNGG 3 cut(s) 131, 1379, 1393
BstSNI TACGTA 1 cut(s) 1302
BstX2I RGATCY 1 cut(s) 678
BstYI RGATCY 1 cut(s) 678
Bsu15I ATCGAT 1 cut(s) 1486
BsuTUI ATCGAT 1 cut(s) 1486
BtgZI GCGATG 1 cut(s) 1268
BtsCI GGATG 5 cut(s) 151, 352, 1118, 1212, 1240
BtsIMutI CAGTG 1 cut(s) 256
BveI ACCTGC 1 cut(s) 1164
Cac8I GCNNGC 1 cut(s) 1571
CciI TCATGA 1 cut(s) 1476
Cfr13I GGNCC 3 cut(s) 297, 601, 1240
ClaI ATCGAT 1 cut(s) 1486
CpoI CGGWCCG 1 cut(s) 601
CseI GACGC 1 cut(s) 5
Csp6I GTAC 3 cut(s) 476, 1303, 1592
CspI CGGWCCG 1 cut(s) 601
CviAII CATG 5 cut(s) 287, 730, 746, 1045, 1477
CviQI GTAC 3 cut(s) 476, 1303, 1592
DdeI CTNAG 2 cut(s) 85, 425
DpnI GATC 7 cut(s) 74, 285, 362, 598, 680, 1485, 1489
DpnII GATC 7 cut(s) 72, 283, 360, 596, 678, 1483, 1487
DrdI GACNNNNNNGTC 1 cut(s) 101
DseDI GACNNNNNNGTC 1 cut(s) 101
Eam1104I CTCTTC 2 cut(s) 836, 1314
EarI CTCTTC 2 cut(s) 836, 1314
Eco105I TACGTA 1 cut(s) 1302
Eco47I GGWCC 3 cut(s) 297, 601, 1240
Eco57I CTGAAG 2 cut(s) 236, 1376
EcoRII CCWGG 2 cut(s) 1379, 1393
Esp3I CGTCTC 1 cut(s) 662
FaeI CATG 5 cut(s) 290, 733, 749, 1048, 1480
FalI AAGNNNNNCTT 2 cut(s) 939, 971
FatI CATG 5 cut(s) 286, 729, 745, 1044, 1476
FauI CCCGC 1 cut(s) 1433
FauNDI CATATG 3 cut(s) 636, 1081, 1513
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 1 cut(s) 14
FokI GGATG 5 cut(s) 158, 359, 1125, 1219, 1247
Fsp4HI GCNGC 1 cut(s) 14
FspBI CTAG 4 cut(s) 351, 783, 914, 1185
GluI GCNGC 1 cut(s) 14
HapII CCGG 1 cut(s) 132
HgaI GACGC 1 cut(s) 5
Hin1II CATG 5 cut(s) 290, 733, 749, 1048, 1480
HincII GTYRAC 1 cut(s) 1260
HindII GTYRAC 1 cut(s) 1260
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 7 cut(s) 119, 167, 365, 416, 742, 1376, 1473
HpaI GTTAAC 1 cut(s) 1260
HpaII CCGG 1 cut(s) 132
HphI GGTGA 2 cut(s) 352, 685
Hpy166II GTNNAC 1 cut(s) 1260
Hpy188III TCNNGA 4 cut(s) 159, 682, 869, 1477
Hpy8I GTNNAC 1 cut(s) 1260
Hpy99I CGWCG 1 cut(s) 21
HpyAV CCTTC 3 cut(s) 211, 698, 774
HpyCH4III ACNGT 2 cut(s) 673, 1269
HpyCH4IV ACGT 3 cut(s) 238, 541, 1301
HpyCH4V TGCA 5 cut(s) 233, 699, 853, 932, 1578
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 1244, 1575
HpyF3I CTNAG 2 cut(s) 85, 425
HpySE526I ACGT 3 cut(s) 238, 541, 1301
Hsp92II CATG 5 cut(s) 290, 733, 749, 1048, 1480
Ksp22I TGATCA 1 cut(s) 283
KspAI GTTAAC 1 cut(s) 1260
Kzo9I GATC 7 cut(s) 72, 283, 360, 596, 678, 1483, 1487
LweI GCATC 6 cut(s) 136, 220, 272, 337, 679, 1225
MaeI CTAG 4 cut(s) 351, 783, 914, 1185
MaeII ACGT 3 cut(s) 238, 541, 1301
MaeIII GTNAC 6 cut(s) 420, 431, 439, 566, 1148, 1383
MalI GATC 7 cut(s) 74, 285, 362, 598, 680, 1485, 1489
MboI GATC 7 cut(s) 72, 283, 360, 596, 678, 1483, 1487
MflI RGATCY 1 cut(s) 678
MhlI GDGCHC 1 cut(s) 244
MluCI AATT 9 cut(s) 21, 36, 176, 491, 919, 1026, 1096, 1227, 1445
MlyI GAGTC 2 cut(s) 1385, 1482
MmeI TCCRAC 3 cut(s) 367, 714, 1192
MroXI GAANNNNTTC 1 cut(s) 1466
MseI TTAA 4 cut(s) 35, 909, 957, 1259
MspCI CTTAAG 1 cut(s) 956
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 3 cut(s) 133, 1381, 1395
Mva1269I GAATGC 1 cut(s) 784
MvaI CCWGG 2 cut(s) 1381, 1395
MwoI GCNNNNNNNGC 3 cut(s) 239, 1244, 1575
NciI CCSGG 1 cut(s) 133
NdeI CATATG 3 cut(s) 636, 1081, 1513
NdeII GATC 7 cut(s) 72, 283, 360, 596, 678, 1483, 1487
NlaIII CATG 5 cut(s) 290, 733, 749, 1048, 1480
NlaIV GGNNCC 1 cut(s) 222
NmuCI GTSAC 1 cut(s) 1383
NspI RCATGY 1 cut(s) 733
NspV TTCGAA 1 cut(s) 449
PagI TCATGA 1 cut(s) 1476
PciI ACATGT 1 cut(s) 729
PctI GAATGC 1 cut(s) 784
PdmI GAANNNNTTC 1 cut(s) 1466
PfeI GAWTC 5 cut(s) 119, 167, 365, 416, 742
PfoI TCCNGGA 1 cut(s) 1393
PkrI GCNGC 1 cut(s) 15
PleI GAGTC 2 cut(s) 1384, 1481
PpsI GAGTC 2 cut(s) 1384, 1481
Ppu21I YACGTR 1 cut(s) 1302
PscI ACATGT 1 cut(s) 729
Psp6I CCWGG 2 cut(s) 1379, 1393
PspGI CCWGG 2 cut(s) 1379, 1393
PspN4I GGNNCC 1 cut(s) 222
PspPI GGNCC 3 cut(s) 297, 601, 1240
PsuI RGATCY 1 cut(s) 678
RsaI GTAC 3 cut(s) 477, 1304, 1593
RsaNI GTAC 3 cut(s) 476, 1303, 1592
Rsr2I CGGWCCG 1 cut(s) 601
RsrII CGGWCCG 1 cut(s) 601
SaqAI TTAA 4 cut(s) 35, 909, 957, 1259
SatI GCNGC 1 cut(s) 14
Sau3AI GATC 7 cut(s) 72, 283, 360, 596, 678, 1483, 1487
Sau96I GGNCC 3 cut(s) 297, 601, 1240
SchI GAGTC 2 cut(s) 1385, 1482
ScrFI CCNGG 3 cut(s) 133, 1381, 1395
SduI GDGCHC 1 cut(s) 244
SfaNI GCATC 6 cut(s) 136, 220, 272, 337, 679, 1225
SfuI TTCGAA 1 cut(s) 449
SinI GGWCC 3 cut(s) 297, 601, 1240
SmlI CTYRAG 1 cut(s) 956
SmoI CTYRAG 1 cut(s) 956
SnaBI TACGTA 1 cut(s) 1302
Sse9I AATT 9 cut(s) 21, 36, 176, 491, 919, 1026, 1096, 1227, 1445
SsiI CCGC 3 cut(s) 715, 1238, 1440
SspI AATATT 1 cut(s) 1401
SspMI CTAG 4 cut(s) 351, 783, 914, 1185
StyD4I CCNGG 3 cut(s) 131, 1379, 1393
TaaI ACNGT 2 cut(s) 673, 1269
TaiI ACGT 3 cut(s) 241, 544, 1304
TaqI TCGA 7 cut(s) 19, 67, 363, 449, 583, 970, 1486
TaqII GACCGA 1 cut(s) 618
TasI AATT 9 cut(s) 21, 36, 176, 491, 919, 1026, 1096, 1227, 1445
TatI WGTACW 2 cut(s) 475, 1591
TfiI GAWTC 5 cut(s) 119, 167, 365, 416, 742
Tru1I TTAA 4 cut(s) 35, 909, 957, 1259
Tru9I TTAA 4 cut(s) 35, 909, 957, 1259
TscAI CASTG 1 cut(s) 263
TseFI GTSAC 1 cut(s) 1383
TseI GCWGC 1 cut(s) 13
Tsp45I GTSAC 1 cut(s) 1383
TspRI CASTG 1 cut(s) 263
Vha464I CTTAAG 1 cut(s) 956
VpaK11BI GGWCC 3 cut(s) 297, 601, 1240
XapI RAATTY 1 cut(s) 919
XceI RCATGY 1 cut(s) 733
XcmI CCANNNNNNNNNTGG 2 cut(s) 319, 340
XmnI GAANNNNTTC 1 cut(s) 1466
XspI CTAG 4 cut(s) 351, 783, 914, 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.