pycom11g19480

CRS2-associated factor 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0003621
Physical Location & Seq
Reverse (-)
22026236 .. 22027178
943 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 738 bp
ATGCTGAAGCAACTCTCATGGCGTCACTCACCTCACAAAACCCTAACCCTAACCCTAGACCCAATCAAACCATTCCTCTCCATCTCCCGCCTTCTGACCCAGTCTCTCTCTGACGACGCATACGAATACGACCCTCCGTTCTCTCCGGTTTCCAAACACTCAAAGCCCAAAAAGAACCTGACTCAGTCTAAAGCTCCGGACCCGAAGAATGACCCGTCCCGGCCTCTCAAATCGGACCTGCCCTTCGACTTCAGCTACTCGTATTCGGAGACCAACCCAGCGGTCGAACCTATTGCGTACCGAGAGTCCCGCAAGTTCTCTCCTTTTGGGCCCGGTCGGCTTGATCGGAAATGGACGGGCACTGTTGCACCGGCCCAGCAAGAAGTGGACTTGGACCGGGTCGCGGAGGAGAGAAAACGGGTTCTCGGAGACCCACTAACGGAGGAGGAAGCGGCGGAGCTGGTGGAACGCTACAGTCATAGTGATTGTTCTCGGCAAATCAATATGGGAAAGGGAGGAGTTACACATAACATGTTAGATGATATCCACAATCATTGGAAGCGAGCCGAGGCGGTGAGGATCAAGTGTTTGGGAGTGCCAACTATTGACATGGACAATGTTTGCTTCCACCTTGGGTGTATATGCAGGACAAGTGCGGTGGGAAGATTATTTATCGGCACATTAATGTCCTTATTTTATATCGGGGTGGAAACTATGATCCGTAGAATCGACCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

246

Amino Acids

27.83

Weight (kDa)

8.23

Isoelectric Point (pI)

48.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 146 - 211 2.4e-10 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 246
AccII CGCG 1 cut(s) 404
AccIII TCCGGA 1 cut(s) 196
AciI CCGC 8 cut(s) 88, 281, 310, 404, 452, 455, 572, 656
AclWI GGATC 2 cut(s) 587, 712
AcuI CTGAAG 2 cut(s) 26, 235
AcyI GRCGYC 1 cut(s) 22
AfaI GTAC 1 cut(s) 299
AfiI CCNNNNNNNGG 2 cut(s) 403, 439
AflIII ACRYGT 1 cut(s) 531
AluBI AGCT 3 cut(s) 194, 255, 460
AluI AGCT 3 cut(s) 194, 255, 460
Alw26I GTCTC 3 cut(s) 108, 263, 423
AlwI GGATC 2 cut(s) 587, 712
Aor13HI TCCGGA 1 cut(s) 196
AoxI GGCC 3 cut(s) 221, 329, 372
ApaI GGGCCC 1 cut(s) 333
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
AseI ATTAAT 1 cut(s) 683
AspS9I GGNCC 6 cut(s) 199, 235, 329, 330, 373, 394
AsuC2I CCSGG 3 cut(s) 220, 333, 398
AsuHPI GGTGA 2 cut(s) 21, 586
AvaII GGWCC 3 cut(s) 199, 235, 394
BaeGI GKGCMC 2 cut(s) 333, 362
BanII GRGCYC 1 cut(s) 333
BccI CCATC 1 cut(s) 89
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BcnI CCSGG 3 cut(s) 220, 333, 398
BcoDI GTCTC 3 cut(s) 108, 263, 423
BfaI CTAG 1 cut(s) 56
BfmI CTRYAG 1 cut(s) 472
BfuAI ACCTGC 1 cut(s) 246
BglI GCCNNNNNGGC 1 cut(s) 337
BisI GCNGC 1 cut(s) 453
BlsI GCNGC 1 cut(s) 454
Bme1390I CCNGG 3 cut(s) 220, 333, 398
Bme18I GGWCC 3 cut(s) 199, 235, 394
BmgT120I GGNCC 6 cut(s) 199, 235, 329, 330, 373, 394
BmiI GGNNCC 2 cut(s) 201, 331
BmrFI CCNGG 3 cut(s) 220, 333, 398
BmrI ACTGGG 1 cut(s) 94
BmuI ACTGGG 1 cut(s) 94
BpuMI CCSGG 3 cut(s) 220, 333, 398
BsaHI GRCGYC 1 cut(s) 22
BsaI GGTCTC 2 cut(s) 263, 423
BsaJI CCNNGG 2 cut(s) 567, 631
BsaWI WCCGGW 2 cut(s) 145, 196
Bsc4I CCNNNNNNNGG 2 cut(s) 403, 439
Bse118I RCCGGY 1 cut(s) 370
Bse1I ACTGG 1 cut(s) 100
BseAI TCCGGA 1 cut(s) 196
BseDI CCNNGG 2 cut(s) 567, 631
BseLI CCNNNNNNNGG 2 cut(s) 403, 439
BseMII CTCAG 1 cut(s) 197
BseNI ACTGG 1 cut(s) 100
BseRI GAGGAG 3 cut(s) 422, 458, 531
BseSI GKGCMC 2 cut(s) 333, 362
BseYI CCCAGC 2 cut(s) 277, 375
Bsh1236I CGCG 1 cut(s) 404
Bsh1285I CGRYCG 2 cut(s) 285, 337
BshFI GGCC 3 cut(s) 223, 331, 374
BsiEI CGRYCG 2 cut(s) 285, 337
BsiSI CCGG 6 cut(s) 146, 197, 220, 333, 371, 397
BslFI GGGAC 2 cut(s) 202, 292
BslI CCNNNNNNNGG 2 cut(s) 403, 439
BsmAI GTCTC 3 cut(s) 108, 263, 423
BsmFI GGGAC 2 cut(s) 202, 292
BsnI GGCC 3 cut(s) 223, 331, 374
Bso31I GGTCTC 2 cut(s) 263, 423
Bsp120I GGGCCC 1 cut(s) 329
Bsp1286I GDGCHC 2 cut(s) 333, 362
Bsp13I TCCGGA 1 cut(s) 196
Bsp143I GATC 3 cut(s) 343, 579, 717
BspACI CCGC 8 cut(s) 88, 281, 310, 404, 452, 455, 572, 656
BspANI GGCC 3 cut(s) 223, 331, 374
BspCNI CTCAG 1 cut(s) 196
BspEI TCCGGA 1 cut(s) 196
BspFNI CGCG 1 cut(s) 404
BspLI GGNNCC 2 cut(s) 201, 331
BspMI ACCTGC 1 cut(s) 246
BspPI GGATC 2 cut(s) 587, 712
BspTNI GGTCTC 2 cut(s) 263, 423
BsrFI RCCGGY 1 cut(s) 370
BsrI ACTGG 1 cut(s) 100
BssAI RCCGGY 1 cut(s) 370
BssECI CCNNGG 2 cut(s) 567, 631
BssMI GATC 3 cut(s) 343, 579, 717
BssNI GRCGYC 1 cut(s) 22
BssT1I CCWWGG 1 cut(s) 631
Bst4CI ACNGT 2 cut(s) 364, 476
BstACI GRCGYC 1 cut(s) 22
BstC8I GCNNGC 1 cut(s) 564
BstDEI CTNAG 1 cut(s) 183
BstFNI CGCG 1 cut(s) 404
BstKTI GATC 3 cut(s) 346, 582, 720
BstMAI GTCTC 3 cut(s) 108, 263, 423
BstMBI GATC 3 cut(s) 343, 579, 717
BstMCI CGRYCG 2 cut(s) 285, 337
BstMWI GCNNNNNNNGC 1 cut(s) 337
BstNSI RCATGY 1 cut(s) 535
BstSCI CCNGG 3 cut(s) 218, 331, 396
BstSFI CTRYAG 1 cut(s) 472
BstSLI GKGCMC 2 cut(s) 333, 362
BstUI CGCG 1 cut(s) 404
BsuRI GGCC 3 cut(s) 223, 331, 374
BtsIMutI CAGTG 1 cut(s) 360
BveI ACCTGC 1 cut(s) 246
Cac8I GCNNGC 1 cut(s) 564
Cfr10I RCCGGY 1 cut(s) 370
Cfr13I GGNCC 6 cut(s) 199, 235, 329, 330, 373, 394
CseI GACGC 2 cut(s) 11, 125
Csp6I GTAC 1 cut(s) 298
CspCI CAANNNNNGTGG 4 cut(s) 536, 571, 639, 674
CviAII CATG 3 cut(s) 18, 532, 610
CviJI RGCY 9 cut(s) 166, 194, 223, 255, 331, 340, 374, 460, 566
CviKI_1 RGCY 9 cut(s) 166, 194, 223, 255, 331, 340, 374, 460, 566
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 1 cut(s) 183
DpnI GATC 3 cut(s) 345, 581, 719
DpnII GATC 3 cut(s) 343, 579, 717
EciI GGCGGA 1 cut(s) 470
Eco130I CCWWGG 1 cut(s) 631
Eco24I GRGCYC 1 cut(s) 333
Eco31I GGTCTC 2 cut(s) 263, 423
Eco32I GATATC 1 cut(s) 544
Eco47I GGWCC 3 cut(s) 199, 235, 394
Eco57I CTGAAG 2 cut(s) 26, 235
EcoRV GATATC 1 cut(s) 544
EcoT14I CCWWGG 1 cut(s) 631
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 631
FaeI CATG 3 cut(s) 21, 535, 613
FaqI GGGAC 2 cut(s) 202, 292
FatI CATG 3 cut(s) 17, 531, 609
FauI CCCGC 2 cut(s) 95, 317
Fnu4HI GCNGC 1 cut(s) 453
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 1 cut(s) 453
FspBI CTAG 1 cut(s) 56
GluI GCNGC 1 cut(s) 453
GsaI CCCAGC 2 cut(s) 281, 379
HaeIII GGCC 3 cut(s) 223, 331, 374
HapII CCGG 6 cut(s) 146, 197, 220, 333, 371, 397
HgaI GACGC 2 cut(s) 11, 125
Hin1I GRCGYC 1 cut(s) 22
Hin1II CATG 3 cut(s) 21, 535, 613
HinfI GANTC 3 cut(s) 181, 305, 726
HpaII CCGG 6 cut(s) 146, 197, 220, 333, 371, 397
HphI GGTGA 2 cut(s) 21, 586
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 6 cut(s) 96, 112, 235, 268, 348, 428
Hpy188III TCNNGA 1 cut(s) 197
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 2 cut(s) 101, 253
HpyCH4III ACNGT 2 cut(s) 364, 476
HpyCH4V TGCA 2 cut(s) 368, 645
HpyF10VI GCNNNNNNNGC 1 cut(s) 337
HpyF3I CTNAG 1 cut(s) 183
Hsp92I GRCGYC 1 cut(s) 22
Hsp92II CATG 3 cut(s) 21, 535, 613
Kpn2I TCCGGA 1 cut(s) 196
Kzo9I GATC 3 cut(s) 343, 579, 717
LmnI GCTCC 2 cut(s) 199, 457
MaeI CTAG 1 cut(s) 56
MaeIII GTNAC 2 cut(s) 23, 520
MalI GATC 3 cut(s) 345, 581, 719
MboI GATC 3 cut(s) 343, 579, 717
MboII GAAGA 2 cut(s) 217, 675
MhlI GDGCHC 2 cut(s) 333, 362
MlyI GAGTC 2 cut(s) 175, 314
MroI TCCGGA 1 cut(s) 196
MseI TTAA 1 cut(s) 683
MslI CAYNNNNRTG 1 cut(s) 683
MspA1I CMGCKG 1 cut(s) 281
MspI CCGG 6 cut(s) 146, 197, 220, 333, 371, 397
MspR9I CCNGG 3 cut(s) 220, 333, 398
MvnI CGCG 1 cut(s) 404
MwoI GCNNNNNNNGC 1 cut(s) 337
NciI CCSGG 3 cut(s) 220, 333, 398
NdeII GATC 3 cut(s) 343, 579, 717
NlaIII CATG 3 cut(s) 21, 535, 613
NlaIV GGNNCC 2 cut(s) 201, 331
NmeAIII GCCGAG 2 cut(s) 472, 592
NmuCI GTSAC 1 cut(s) 23
NspI RCATGY 1 cut(s) 535
PciI ACATGT 1 cut(s) 531
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PfeI GAWTC 1 cut(s) 726
PflFI GACNNNGTC 3 cut(s) 100, 184, 398
PkrI GCNGC 1 cut(s) 454
PleI GAGTC 2 cut(s) 175, 313
PpsI GAGTC 2 cut(s) 175, 313
PscI ACATGT 1 cut(s) 531
PshBI ATTAAT 1 cut(s) 683
PspFI CCCAGC 2 cut(s) 277, 375
PspN4I GGNNCC 2 cut(s) 201, 331
PspOMI GGGCCC 1 cut(s) 329
PspPI GGNCC 6 cut(s) 199, 235, 329, 330, 373, 394
PsyI GACNNNGTC 3 cut(s) 100, 184, 398
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
RseI CAYNNNNRTG 1 cut(s) 683
SaqAI TTAA 1 cut(s) 683
SatI GCNGC 1 cut(s) 453
Sau3AI GATC 3 cut(s) 343, 579, 717
Sau96I GGNCC 6 cut(s) 199, 235, 329, 330, 373, 394
SchI GAGTC 2 cut(s) 175, 314
ScrFI CCNGG 3 cut(s) 220, 333, 398
SduI GDGCHC 2 cut(s) 333, 362
SetI ASST 9 cut(s) 34, 180, 196, 240, 257, 292, 462, 633, 735
SfcI CTRYAG 1 cut(s) 472
SinI GGWCC 3 cut(s) 199, 235, 394
SmiMI CAYNNNNRTG 1 cut(s) 683
SsiI CCGC 8 cut(s) 88, 281, 310, 404, 452, 455, 572, 656
SspMI CTAG 1 cut(s) 56
StyD4I CCNGG 3 cut(s) 218, 331, 396
StyI CCWWGG 1 cut(s) 631
TaaI ACNGT 2 cut(s) 364, 476
TaqI TCGA 3 cut(s) 246, 285, 729
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 1 cut(s) 726
Tru1I TTAA 1 cut(s) 683
Tru9I TTAA 1 cut(s) 683
TscAI CASTG 1 cut(s) 367
TseFI GTSAC 1 cut(s) 23
Tsp45I GTSAC 1 cut(s) 23
TspGWI ACGGA 3 cut(s) 126, 455, 710
TspRI CASTG 1 cut(s) 367
Tth111I GACNNNGTC 3 cut(s) 100, 184, 398
VpaK11BI GGWCC 3 cut(s) 199, 235, 394
VspI ATTAAT 1 cut(s) 683
XceI RCATGY 1 cut(s) 535
XspI CTAG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.