pycom11g19980

N-terminal region of Chorein or VPS13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007959
Physical Location & Seq
Reverse (-)
22523982 .. 22533373
9392 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3477 bp
ATGGAGTCGATATTGGCTCGGGCGCTGGAGTACACTCTCAAGTACTGGTTGAAATCCTTCTCCAGAGACCAGTTCAAGTTGCAGGGACGGACGGTTCAGCTTTCTAATTTGGATATCAATGGCGATGCTTTGCACGCGAGCATGGGATTGCCGCCGGCACTGAATGTAGCCACGGCCAAAGTTGGAAAATTGGAGATAGTGCTCCCATCTGTGGGTAATGTGCAAATAGAGCCGATTGTTGTGCAAATTGATAGGCTTGATTTGGTTTTGGAGGAGAAGTCAGATTTGGATGAGAGGAGTCCAAGAAGTTCCCCATCTTCCTCTAGCTCAGCAAAGGGTAGCGGTTATGGATTTGCTGATAAGATTGCGGATGGAATGACTTTGGAGATTCTCACAGTTAATCTTCTACTTGAGACTCGTGGGGGTGGTCGAAGCCAAGGAGGCGCTAGTTGGGCATCACCTCTGGCCTCTATAACCATACACAACCTTTTGCTGTATACTACCAATGAAAATTGGCAGGTTGTAAATCTTAAGGAGGCACGGGAATTCTCCAGCGACAAAAATTTCATATATTTGTTCAAAAAACTTGAGTGGGAATCTTTGTCTATTGATCTCCTTCCCCACCCTGATATGTTCATGGATGCTAACATAGCACGTACTGAAGATGGAAGAAACCAGAGAGACGACGATGGTGCAAAGCGAGTTTTCTTTGGTGGGGAGCGTTTTATAGAAGGAATTTCAGGACAAGCTAATATCACACTGCAAAGGACTGAATTAAACAGTCCACTTGGACTTGAGGTTCAGTTACATATCACAGAGGCCATTTGTCCTGCTATAAGTGAACCAGGACTACGTGCTCTTCTCCGCTTTATGACAGGATTGTATGTCTGTCTGAATAGAGGAGATGTTGATTCAAATACTCAGCAGCGATCCACAGAAGCAGCAGGACGTTCTGTAGTCTCAATTGTTGTGGACCACATATTTCTCTGCATTAAAGATGCTGAGTTCAAGCTCGAACTTTTAATGCAATCACTCTTCTTCTCACGGGCCAGTGTTTCAGATGGAGAAATTGACAACAACTTGTCGAGGGTTATGATTGGTGGACTGTTTTTAAGGGATACCTTTTCGCGGCCTCCATGCACTTTGGTTCAACCATCAATGCATGCAGTTTCTGAAGAACCTCTGCATATTGATCTCCAAGAAGGATCGTGTTTGAGGATCGCTTCCTTCCTGGCTGATGGAATTGTGGTCAATCCTGGTGCTGTTGTACCAGATTCCTCAGTAAATTCCTTGATTTTCACCCTCAAGGAGTTAGATGTGACTGTTCCTTTGGACATAGATAAGTTGTGTGACTCTGCTAACAACAGGGACAATATCTACCAGAGTGCCTTTTCTGGAGCAAGGCTTCATATCAAGAACTTGTTATTTTCGGAGTCACCATCACTAAAACTAAGGTTACTGAATCTGGAGAAGGACCCTGCTTGCTTTTGTCTTTGGGAAGGTCAACCAATTGATGCCAGCCAGAAGAAATGGAGTGCTAGAGCATCACACATTAGTTTGTCATTAGAAAAGTGCACTAAGTCAGCTGGCCTTCAGAGTTCTATTGATTGGAATTCAGGCATGTGGAGATGTGTTGAGCTGAAAGATGCTTGTGTTGAAGTAGCTATGGTAACTGCTGATGGGAGCCCATTAACAAATGTTCCTCCTCCCGGTGGTATTGTCAGGGTGGGGGTAGCTTGGCAAAACTATCTCTCCAACACATCTGTTGAACAGTTATTTTTTGTTATGGATCTTTATTCGTACTTTGGCAGAGTTAGTGAAAAAATTGTTCTCGTTGGAAAAAATACTGGAAAAAAGAAGAAGAGGGATCATTCTGTTGAATTAAAGCTGATTGACAAGGTTCCCAATGATACAGCGGTAAGCTTGGCAGTCAAAAATCTCCAGATTAAATTTCTGGAGTCATCTTCCATGAATATTGAAGGAATGCCTCTGGTTCAGTTTATTGGTGATGATCTCTTCATAAAAGTTACTCACAGAACTCTTGGTGGTGCAATTGCTGTTTCATCCACTATTCATTGGGATAGTGTTGAGGTGGACTGTGTAGACACTGAGGGAAACTTGGGACATGAGAATGGCTCAGGTTTAACTTATGTCGAAAATGGTCTTTCAACCAGTGGAAATGGATACCCTCAGCTAAGACCTGTATTTTGGATACATAACCAAACAAAACATCAATCAAATGGCAAGGCTTTTGTGGATCCATTTCTGGACATAAGTATGGTACACGTAATTCCTTTAAATGAACGTGATGCAGAGTGTCATAGTCTGAATGTGTGTGCGTCTATCTCTGGCATACGCCTAGGTGGGGGAATGAACTATGCTGAATCCTTGCTGCATCGCTTTGGAATACTTGGCCCTGATGGTGGTCCTGGAAAGGGGCTCTCTAAGGAGTTAGAAAAATTGCAAGCTGGACCACTGTCGGAACTTTTCAAACCGTCGCATCTCATTATTGATCTGAAAGAGGATAGAAGTTCACGAGACGGGAAAGAAAGTGGGGTTTTGCACTTGGGAAAACCAGATGATGTCGACGTGTCCATAGAGTTCAAAAATTGGTTATTTGCTCTTGAAGGTGAACGGGAGATTGCAGAAAGATGGTGGTTTGATAATCATGAGGATGTGCAAAGAGAAGAGAGGTGTTGGCACACAACGTTCCATAATCTGCATGTAAAAGCAAAAAGTAGTCCAAAGCATAGGCTGAGTGGCAATGGACAGTCTTACAGGGCCCAAAAATATCCTGTGGAATTGGTTATAGTTGGTGTGCAAGGCTTGCAGATGCTAAAGCCCCATGCTCAGAAGTCTAACAATGCTGCTATCTTACCTGCGAATGGGATCAAGGAAACTACTGAGACATCTGGAGGTATAGATCTTGAAATTCGCATGGTGATACCAGAGGACCCAGTTGATCATGAAATGGTGGTATGGGCTGTGGAAAATGTGAAATTCTCTGTTAAGCAACCGATTGAGGCGGTTGTAACAAAGGATGAGTTGCAACATCTTACTTTTCTGTGCAAGTCGGAAGTTGAATCAATGGGTCGAGTCACTGCTGGAATTCTGAGGCTACTCAAGCTGGAGGGTTCCATTGGCGAGGCTGCAATGGAACAACTGAGCAACCTCGGAACAGAGGGCATTGACAAGATATTCTCCCCTGGAAAGCTCACCAGGGGTGGCAGCTTTTCCAGTACTGGTCTCTCTCAATCAAACTTGGTTAATGGAACCCCAAGTACAACCGCCACCTTGGAATCGACAGTGGCTTCACTCGAGGAGGCATTTACAGAATCACAGGCCAAGTGCACTGCTCTTCTTGCCGACCTAGATAGTTCAGAACAAGCCGTGCATCATCTTGCAACTGTAAAACAACTCAATGAGAAACTTCAGAGTATGGAGAGTTTATTGATGCAATTACGGTTGCAGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chorein_N

Protein Analysis

1159

Amino Acids

127.29

Weight (kDa)

5.45

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BLTP3A_B PF24917 19 - 204 2.8e-07 Bridge-like lipid transfer protein family member 3A/B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 508, 2887
AccI GTMKAC 3 cut(s) 497, 2103, 2586
AccII CGCG 2 cut(s) 137, 1129
AciI CCGC 8 cut(s) 152, 342, 368, 865, 1129, 1916, 3026, 3288
AclI AACGTT 1 cut(s) 2708
AclWI GGATC 8 cut(s) 924, 1213, 1226, 1797, 1875, 2252, 2265, 2897
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 9 cut(s) 545, 562, 735, 1285, 1612, 1949, 2931, 2999, 3108
AcuI CTGAAG 4 cut(s) 681, 1194, 1577, 3416
AfaI GTAC 8 cut(s) 32, 44, 658, 1269, 1802, 2283, 3241, 3283
AfiI CCNNNNNNNGG 9 cut(s) 211, 212, 1128, 1709, 1712, 2365, 2423, 2435, 2885
AflII CTTAAG 1 cut(s) 530
AflIII ACRYGT 2 cut(s) 2284, 2589
AjiI CACGTC 1 cut(s) 2590
AjnI CCWGG 6 cut(s) 844, 1230, 1255, 2428, 3205, 3218
AjuI GAANNNNNNNTTGG 2 cut(s) 269, 301
AloI GAACNNNNNNTCC 4 cut(s) 78, 110, 783, 815
Alw21I GWGCWC 4 cut(s) 204, 859, 1577, 3353
Alw26I GTCTC 7 cut(s) 60, 407, 675, 964, 2532, 2900, 3251
Alw44I GTGCAC 2 cut(s) 1573, 3349
AlwI GGATC 8 cut(s) 924, 1213, 1226, 1797, 1875, 2252, 2265, 2897
AlwNI CAGNNNCTG 1 cut(s) 1172
Ama87I CYCGRG 2 cut(s) 18, 3317
AoxI GGCC 9 cut(s) 174, 465, 819, 1047, 1130, 1588, 2413, 2781, 3342
ApaI GGGCCC 1 cut(s) 2785
ApaLI GTGCAC 2 cut(s) 1573, 3349
ApeKI GCWGC 6 cut(s) 925, 941, 2392, 2867, 3149, 3228
ApoI RAATTY 9 cut(s) 545, 562, 735, 1285, 1612, 1949, 2931, 2999, 3108
ArsI GACNNNNNNTTYG 4 cut(s) 1312, 1344, 2190, 2222
Asp700I GAANNNNTTC 1 cut(s) 56
AspA2I CCTAGG 1 cut(s) 2359
AspLEI GCGC 2 cut(s) 25, 446
AspS9I GGNCC 9 cut(s) 973, 1047, 1474, 2414, 2426, 2471, 2781, 2782, 2953
AsuC2I CCSGG 1 cut(s) 1710
AsuHPI GGTGA 7 cut(s) 450, 1291, 1428, 2018, 2642, 2953, 3208
AvaI CYCGRG 2 cut(s) 18, 3317
AvaII GGWCC 5 cut(s) 973, 1474, 2426, 2471, 2953
AvrII CCTAGG 1 cut(s) 2359
BaeGI GKGCMC 3 cut(s) 1577, 2785, 3353
BaeI ACNNNNGTAYC 2 cut(s) 1902, 1935
BamHI GGATCC 1 cut(s) 2257
BanII GRGCYC 3 cut(s) 1688, 2442, 2785
BauI CACGAG 2 cut(s) 417, 2535
Bbv12I GWGCWC 4 cut(s) 204, 859, 1577, 3353
BbvCI CCTCAGC 1 cut(s) 2190
BbvI GCAGC 6 cut(s) 937, 953, 2379, 2854, 3136, 3240
BceAI ACGGC 2 cut(s) 189, 3374
BcgI CGANNNNNNTGC 4 cut(s) 223, 257, 674, 708
BciT130I CCWGG 6 cut(s) 846, 1232, 1257, 2430, 3207, 3220
BciVI GTATCC 3 cut(s) 1111, 2177, 2205
BclI TGATCA 1 cut(s) 2962
BcnI CCSGG 1 cut(s) 1710
BcoDI GTCTC 7 cut(s) 60, 407, 675, 964, 2532, 2900, 3251
BfaI CTAG 5 cut(s) 324, 447, 1539, 2360, 3371
BfmI CTRYAG 1 cut(s) 954
BfoI RGCGCY 2 cut(s) 26, 447
BfrI CTTAAG 1 cut(s) 530
BfuAI ACCTGC 2 cut(s) 508, 2887
BfuI GTATCC 3 cut(s) 1111, 2177, 2205
BglI GCCNNNNNGGC 1 cut(s) 441
BglII AGATCT 1 cut(s) 2923
BisI GCNGC 8 cut(s) 152, 926, 942, 1130, 2393, 2868, 3150, 3229
BlnI CCTAGG 1 cut(s) 2359
BlpI GCTNAGC 1 cut(s) 328
BlsI GCNGC 8 cut(s) 153, 927, 943, 1131, 2394, 2869, 3151, 3230
BmcAI AGTACT 2 cut(s) 44, 3241
Bme1390I CCNGG 7 cut(s) 846, 1232, 1257, 1710, 2430, 3207, 3220
Bme18I GGWCC 5 cut(s) 973, 1474, 2426, 2471, 2953
BmeT110I CYCGRG 2 cut(s) 18, 3317
BmgBI CACGTC 1 cut(s) 2590
BmgT120I GGNCC 9 cut(s) 973, 1047, 1474, 2414, 2426, 2471, 2781, 2782, 2953
BmiI GGNNCC 8 cut(s) 1476, 1685, 1902, 2259, 2783, 2955, 3136, 3274
BmrFI CCNGG 7 cut(s) 846, 1232, 1257, 1710, 2430, 3207, 3220
BmrI ACTGGG 1 cut(s) 2951
BmuI ACTGGG 1 cut(s) 2951
BoxI GACNNNNGTC 1 cut(s) 2476
BplI GAGNNNNNCTC 2 cut(s) 2120, 2152
BpmI CTGGAG 9 cut(s) 46, 47, 535, 1416, 1487, 1925, 1976, 2934, 3149
Bpu10I CCTNAGC 2 cut(s) 2137, 2190
Bpu1102I GCTNAGC 1 cut(s) 328
BpuEI CTTGAG 6 cut(s) 23, 431, 608, 815, 1289, 3107
BpuMI CCSGG 1 cut(s) 1710
BsaAI YACGTR 3 cut(s) 656, 854, 2287
BsaI GGTCTC 2 cut(s) 60, 3251
BsaJI CCNNGG 7 cut(s) 171, 436, 2359, 3172, 3205, 3219, 3294
BsaXI ACNNNNNCTCC 4 cut(s) 263, 293, 1736, 1766
Bsc4I CCNNNNNNNGG 9 cut(s) 211, 212, 1128, 1709, 1712, 2365, 2423, 2435, 2885
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 8 cut(s) 50, 70, 1050, 1852, 2172, 2957, 3237, 3247
Bse3DI GCAATG 2 cut(s) 2770, 3159
BseBI CCWGG 6 cut(s) 846, 1232, 1257, 2430, 3207, 3220
BseDI CCNNGG 7 cut(s) 171, 436, 2359, 3172, 3205, 3219, 3294
BseGI GGATG 6 cut(s) 295, 376, 646, 2063, 2680, 3046
BseLI CCNNNNNNNGG 9 cut(s) 211, 212, 1128, 1709, 1712, 2365, 2423, 2435, 2885
BseMI GCAATG 2 cut(s) 2770, 3159
BseNI ACTGG 8 cut(s) 50, 70, 1050, 1852, 2172, 2957, 3237, 3247
BseRI GAGGAG 5 cut(s) 287, 310, 915, 1695, 3335
BseSI GKGCMC 3 cut(s) 1577, 2785, 3353
BseXI GCAGC 6 cut(s) 937, 953, 2379, 2854, 3136, 3240
Bsh1236I CGCG 2 cut(s) 137, 1129
BshFI GGCC 9 cut(s) 176, 467, 821, 1049, 1132, 1590, 2415, 2783, 3344
BsiHKAI GWGCWC 4 cut(s) 204, 859, 1577, 3353
BsiHKCI CYCGRG 2 cut(s) 18, 3317
BsiSI CCGG 2 cut(s) 155, 1710
BslFI GGGAC 3 cut(s) 99, 1382, 2136
BslI CCNNNNNNNGG 9 cut(s) 211, 212, 1128, 1709, 1712, 2365, 2423, 2435, 2885
BsmAI GTCTC 7 cut(s) 60, 407, 675, 964, 2532, 2900, 3251
BsmBI CGTCTC 2 cut(s) 675, 2532
BsmFI GGGAC 3 cut(s) 99, 1382, 2136
BsmI GAATGC 1 cut(s) 1989
BsnI GGCC 9 cut(s) 176, 467, 821, 1049, 1132, 1590, 2415, 2783, 3344
Bso31I GGTCTC 2 cut(s) 60, 3251
BsoBI CYCGRG 2 cut(s) 18, 3317
Bsp120I GGGCCC 1 cut(s) 2781
Bsp1286I GDGCHC 7 cut(s) 204, 859, 1577, 1688, 2442, 2785, 3353
Bsp1720I GCTNAGC 1 cut(s) 328
BspACI CCGC 8 cut(s) 152, 342, 368, 865, 1129, 1916, 3026, 3288
BspANI GGCC 9 cut(s) 176, 467, 821, 1049, 1132, 1590, 2415, 2783, 3344
BspFNI CGCG 2 cut(s) 137, 1129
BspHI TCATGA 2 cut(s) 2668, 2965
BspLI GGNNCC 8 cut(s) 1476, 1685, 1902, 2259, 2783, 2955, 3136, 3274
BspMI ACCTGC 2 cut(s) 508, 2887
BspPI GGATC 8 cut(s) 924, 1213, 1226, 1797, 1875, 2252, 2265, 2897
BspQI GCTCTTC 2 cut(s) 864, 3363
BspTI CTTAAG 1 cut(s) 530
BspTNI GGTCTC 2 cut(s) 60, 3251
BsrDI GCAATG 2 cut(s) 2770, 3159
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 8 cut(s) 50, 70, 1050, 1852, 2172, 2957, 3237, 3247
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 7 cut(s) 171, 436, 2359, 3172, 3205, 3219, 3294
BssNAI GTATAC 1 cut(s) 498
BssSI CACGAG 2 cut(s) 417, 2535
BssT1I CCWWGG 3 cut(s) 436, 2359, 3294
Bst1107I GTATAC 1 cut(s) 498
Bst2BI CACGAG 2 cut(s) 417, 2535
Bst2UI CCWGG 6 cut(s) 846, 1232, 1257, 2430, 3207, 3220
Bst6I CTCTTC 6 cut(s) 864, 1040, 1856, 2021, 2682, 3363
BstAFI CTTAAG 1 cut(s) 530
BstAPI GCANNNNNTGC 1 cut(s) 2827
BstBAI YACGTR 3 cut(s) 656, 854, 2287
BstC8I GCNNGC 9 cut(s) 135, 139, 156, 1164, 1483, 1519, 1588, 2466, 2828
BstDSI CCRYGG 1 cut(s) 171
BstF5I GGATG 6 cut(s) 295, 376, 646, 2063, 2680, 3046
BstFNI CGCG 2 cut(s) 137, 1129
BstH2I RGCGCY 2 cut(s) 26, 447
BstHHI GCGC 2 cut(s) 25, 446
BstMAI GTCTC 7 cut(s) 60, 407, 675, 964, 2532, 2900, 3251
BstMWI GCNNNNNNNGC 8 cut(s) 134, 229, 441, 452, 650, 2827, 3124, 3362
BstNI CCWGG 6 cut(s) 846, 1232, 1257, 2430, 3207, 3220
BstNSI RCATGY 3 cut(s) 1166, 1624, 2726
BstPAI GACNNNNGTC 1 cut(s) 2476
BstSCI CCNGG 7 cut(s) 844, 1230, 1255, 1708, 2428, 3205, 3218
BstSFI CTRYAG 1 cut(s) 954
BstSLI GKGCMC 3 cut(s) 1577, 2785, 3353
BstUI CGCG 2 cut(s) 137, 1129
BstV1I GCAGC 6 cut(s) 937, 953, 2379, 2854, 3136, 3240
BstX2I RGATCY 3 cut(s) 1789, 2257, 2923
BstYI RGATCY 3 cut(s) 1789, 2257, 2923
BstZ17I GTATAC 1 cut(s) 498
BsuI GTATCC 3 cut(s) 1111, 2177, 2205
BsuRI GGCC 9 cut(s) 176, 467, 821, 1049, 1132, 1590, 2415, 2783, 3344
BtgI CCRYGG 1 cut(s) 171
BtgZI GCGATG 2 cut(s) 138, 2381
BtrI CACGTC 1 cut(s) 2590
BtsCI GGATG 6 cut(s) 295, 376, 646, 2063, 2680, 3046
BtsI GCAGTG 3 cut(s) 758, 3099, 3351
BtsIMutI CAGTG 9 cut(s) 158, 758, 1057, 2106, 2179, 2474, 3099, 3312, 3351
BveI ACCTGC 2 cut(s) 508, 2887
Cac8I GCNNGC 9 cut(s) 135, 139, 156, 1164, 1483, 1519, 1588, 2466, 2828
CaiI CAGNNNCTG 1 cut(s) 1172
CciI TCATGA 2 cut(s) 2668, 2965
CfoI GCGC 2 cut(s) 25, 446
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 9 cut(s) 973, 1047, 1474, 2414, 2426, 2471, 2781, 2782, 2953
CseI GACGC 1 cut(s) 2328
Csp6I GTAC 8 cut(s) 31, 43, 657, 1268, 1801, 2282, 3240, 3282
CspCI CAANNNNNGTGG 2 cut(s) 951, 986
CviQI GTAC 8 cut(s) 31, 43, 657, 1268, 1801, 2282, 3240, 3282
DraI TTTAAA 1 cut(s) 2298
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 6 cut(s) 864, 1040, 1856, 2021, 2682, 3363
EarI CTCTTC 6 cut(s) 864, 1040, 1856, 2021, 2682, 3363
Eco130I CCWWGG 3 cut(s) 436, 2359, 3294
Eco24I GRGCYC 3 cut(s) 1688, 2442, 2785
Eco31I GGTCTC 2 cut(s) 60, 3251
Eco32I GATATC 1 cut(s) 115
Eco47I GGWCC 5 cut(s) 973, 1474, 2426, 2471, 2953
Eco57I CTGAAG 4 cut(s) 681, 1194, 1577, 3416
Eco88I CYCGRG 2 cut(s) 18, 3317
EcoO109I RGGNCCY 3 cut(s) 1474, 2781, 2953
EcoRI GAATTC 3 cut(s) 545, 1612, 3108
EcoRII CCWGG 6 cut(s) 844, 1230, 1255, 2428, 3205, 3218
EcoRV GATATC 1 cut(s) 115
EcoT14I CCWWGG 3 cut(s) 436, 2359, 3294
EcoT22I ATGCAT 1 cut(s) 1164
EcoT38I GRGCYC 3 cut(s) 1688, 2442, 2785
ErhI CCWWGG 3 cut(s) 436, 2359, 3294
Esp3I CGTCTC 2 cut(s) 675, 2532
FaqI GGGAC 3 cut(s) 99, 1382, 2136
FbaI TGATCA 1 cut(s) 2962
FblI GTMKAC 3 cut(s) 497, 2103, 2586
Fnu4HI GCNGC 8 cut(s) 152, 926, 942, 1130, 2393, 2868, 3150, 3229
FokI GGATG 6 cut(s) 302, 383, 653, 2050, 2687, 3053
FriOI GRGCYC 3 cut(s) 1688, 2442, 2785
Fsp4HI GCNGC 8 cut(s) 152, 926, 942, 1130, 2393, 2868, 3150, 3229
FspBI CTAG 5 cut(s) 324, 447, 1539, 2360, 3371
GlaI GCGC 2 cut(s) 24, 445
GluI GCNGC 8 cut(s) 152, 926, 942, 1130, 2393, 2868, 3150, 3229
GsuI CTGGAG 9 cut(s) 46, 47, 535, 1416, 1487, 1925, 1976, 2934, 3149
HaeII RGCGCY 2 cut(s) 26, 447
HaeIII GGCC 9 cut(s) 176, 467, 821, 1049, 1132, 1590, 2415, 2783, 3344
HapII CCGG 2 cut(s) 155, 1710
HgaI GACGC 1 cut(s) 2328
HhaI GCGC 2 cut(s) 25, 446
Hin6I GCGC 2 cut(s) 23, 444
HinP1I GCGC 2 cut(s) 23, 444
HincII GTYRAC 2 cut(s) 1505, 2587
HindII GTYRAC 2 cut(s) 1505, 2587
HindIII AAGCTT 1 cut(s) 1921
HpaII CCGG 2 cut(s) 155, 1710
HphI GGTGA 7 cut(s) 450, 1291, 1428, 2018, 2642, 2953, 3208
Hpy99I CGWCG 3 cut(s) 689, 2500, 2591
HpyCH4IV ACGT 7 cut(s) 655, 853, 949, 2286, 2305, 2589, 2708
HpyF10VI GCNNNNNNNGC 8 cut(s) 134, 229, 441, 452, 650, 2827, 3124, 3362
HpySE526I ACGT 7 cut(s) 655, 853, 949, 2286, 2305, 2589, 2708
HspAI GCGC 2 cut(s) 23, 444
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
Ksp22I TGATCA 1 cut(s) 2962
LguI GCTCTTC 2 cut(s) 864, 3363
LmnI GCTCC 4 cut(s) 207, 718, 1397, 1683
Lsp1109I GCAGC 6 cut(s) 937, 953, 2379, 2854, 3136, 3240
MaeI CTAG 5 cut(s) 324, 447, 1539, 2360, 3371
MaeII ACGT 7 cut(s) 655, 853, 949, 2286, 2305, 2589, 2708
MaeIII GTNAC 9 cut(s) 804, 1318, 1349, 1434, 1455, 1669, 2026, 3031, 3097
MfeI CAATTG 3 cut(s) 963, 1509, 2052
MflI RGATCY 3 cut(s) 1789, 2257, 2923
MhlI GDGCHC 7 cut(s) 204, 859, 1577, 1688, 2442, 2785, 3353
MlyI GAGTC 7 cut(s) 14, 307, 409, 1346, 1442, 1967, 3105
MmeI TCCRAC 5 cut(s) 163, 1779, 1816, 2460, 3054
Mph1103I ATGCAT 1 cut(s) 1164
MroNI GCCGGC 1 cut(s) 154
MroXI GAANNNNTTC 1 cut(s) 56
MslI CAYNNNNRTG 3 cut(s) 2130, 2276, 2673
MspA1I CMGCKG 2 cut(s) 1586, 1916
MspCI CTTAAG 1 cut(s) 530
MspI CCGG 2 cut(s) 155, 1710
MspR9I CCNGG 7 cut(s) 846, 1232, 1257, 1710, 2430, 3207, 3220
MunI CAATTG 3 cut(s) 963, 1509, 2052
Mva1269I GAATGC 1 cut(s) 1989
MvaI CCWGG 6 cut(s) 846, 1232, 1257, 2430, 3207, 3220
MvnI CGCG 2 cut(s) 137, 1129
MwoI GCNNNNNNNGC 8 cut(s) 134, 229, 441, 452, 650, 2827, 3124, 3362
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 1 cut(s) 1710
NgoMIV GCCGGC 1 cut(s) 154
NlaIV GGNNCC 8 cut(s) 1476, 1685, 1902, 2259, 2783, 2955, 3136, 3274
NmuCI GTSAC 4 cut(s) 1318, 1349, 1434, 3097
NsiI ATGCAT 1 cut(s) 1164
NspI RCATGY 3 cut(s) 1166, 1624, 2726
PaeI GCATGC 1 cut(s) 1166
PaeR7I CTCGAG 1 cut(s) 3317
PagI TCATGA 2 cut(s) 2668, 2965
PciSI GCTCTTC 2 cut(s) 864, 3363
PctI GAATGC 1 cut(s) 1989
PdiI GCCGGC 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 9 cut(s) 388, 596, 911, 1274, 1462, 2384, 3083, 3299, 3335
PfoI TCCNGGA 1 cut(s) 2428
PkrI GCNGC 8 cut(s) 153, 927, 943, 1131, 2394, 2869, 3151, 3230
PleI GAGTC 7 cut(s) 13, 306, 409, 1346, 1441, 1966, 3104
PpsI GAGTC 7 cut(s) 13, 306, 409, 1346, 1441, 1966, 3104
Ppu21I YACGTR 3 cut(s) 656, 854, 2287
PpuMI RGGWCCY 2 cut(s) 1474, 2953
PshAI GACNNNNGTC 1 cut(s) 2476
Psp1406I AACGTT 1 cut(s) 2708
Psp5II RGGWCCY 2 cut(s) 1474, 2953
Psp6I CCWGG 6 cut(s) 844, 1230, 1255, 2428, 3205, 3218
PspGI CCWGG 6 cut(s) 844, 1230, 1255, 2428, 3205, 3218
PspN4I GGNNCC 8 cut(s) 1476, 1685, 1902, 2259, 2783, 2955, 3136, 3274
PspOMI GGGCCC 1 cut(s) 2781
PspPI GGNCC 9 cut(s) 973, 1047, 1474, 2414, 2426, 2471, 2781, 2782, 2953
PspPPI RGGWCCY 2 cut(s) 1474, 2953
PspXI VCTCGAGB 1 cut(s) 3317
PsrI GAACNNNNNNTAC 4 cut(s) 834, 866, 3265, 3297
PstNI CAGNNNCTG 1 cut(s) 1172
PsuI RGATCY 3 cut(s) 1789, 2257, 2923
PvuII CAGCTG 1 cut(s) 1586
RsaI GTAC 8 cut(s) 32, 44, 658, 1269, 1802, 2283, 3241, 3283
RsaNI GTAC 8 cut(s) 31, 43, 657, 1268, 1801, 2282, 3240, 3282
RseI CAYNNNNRTG 3 cut(s) 2130, 2276, 2673
SalI GTCGAC 1 cut(s) 2585
SapI GCTCTTC 2 cut(s) 864, 3363
SatI GCNGC 8 cut(s) 152, 926, 942, 1130, 2393, 2868, 3150, 3229
Sau96I GGNCC 9 cut(s) 973, 1047, 1474, 2414, 2426, 2471, 2781, 2782, 2953
ScaI AGTACT 2 cut(s) 44, 3241
SchI GAGTC 7 cut(s) 14, 307, 409, 1346, 1442, 1967, 3105
ScrFI CCNGG 7 cut(s) 846, 1232, 1257, 1710, 2430, 3207, 3220
SduI GDGCHC 7 cut(s) 204, 859, 1577, 1688, 2442, 2785, 3353
SfcI CTRYAG 1 cut(s) 954
Sfr274I CTCGAG 1 cut(s) 3317
SinI GGWCC 5 cut(s) 973, 1474, 2426, 2471, 2953
SlaI CTCGAG 1 cut(s) 3317
SmiMI CAYNNNNRTG 3 cut(s) 2130, 2276, 2673
SmlI CTYRAG 8 cut(s) 38, 410, 530, 587, 794, 1304, 3122, 3317
SmoI CTYRAG 8 cut(s) 38, 410, 530, 587, 794, 1304, 3122, 3317
SphI GCATGC 1 cut(s) 1166
SsiI CCGC 8 cut(s) 152, 342, 368, 865, 1129, 1916, 3026, 3288
SspI AATATT 1 cut(s) 1975
SspMI CTAG 5 cut(s) 324, 447, 1539, 2360, 3371
StyD4I CCNGG 7 cut(s) 844, 1230, 1255, 1708, 2428, 3205, 3218
StyI CCWWGG 3 cut(s) 436, 2359, 3294
TaiI ACGT 7 cut(s) 658, 856, 952, 2289, 2308, 2592, 2711
TaqI TCGA 9 cut(s) 8, 430, 1014, 1085, 2154, 2586, 3094, 3302, 3318
TatI WGTACW 4 cut(s) 30, 42, 3239, 3281
TauI GCSGC 2 cut(s) 154, 1132
TfiI GAWTC 9 cut(s) 388, 596, 911, 1274, 1462, 2384, 3083, 3299, 3335
TscAI CASTG 9 cut(s) 165, 765, 1057, 2113, 2179, 2481, 3106, 3312, 3358
TseFI GTSAC 4 cut(s) 1318, 1349, 1434, 3097
TseI GCWGC 6 cut(s) 925, 941, 2392, 2867, 3149, 3228
Tsp45I GTSAC 4 cut(s) 1318, 1349, 1434, 3097
TspGWI ACGGA 1 cut(s) 103
TspRI CASTG 9 cut(s) 165, 765, 1057, 2113, 2179, 2481, 3106, 3312, 3358
Vha464I CTTAAG 1 cut(s) 530
VneI GTGCAC 2 cut(s) 1573, 3349
VpaK11BI GGWCC 5 cut(s) 973, 1474, 2426, 2471, 2953
XapI RAATTY 9 cut(s) 545, 562, 735, 1285, 1612, 1949, 2931, 2999, 3108
XceI RCATGY 3 cut(s) 1166, 1624, 2726
XhoI CTCGAG 1 cut(s) 3317
XmaJI CCTAGG 1 cut(s) 2359
XmiI GTMKAC 3 cut(s) 497, 2103, 2586
XmnI GAANNNNTTC 1 cut(s) 56
XspI CTAG 5 cut(s) 324, 447, 1539, 2360, 3371
ZrmI AGTACT 2 cut(s) 44, 3241
Zsp2I ATGCAT 1 cut(s) 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.