pycom11g24760

Auxin canalisation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0009825
Physical Location & Seq
Reverse (-)
27633621 .. 27637348
3728 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1278 bp
ATGTATGGAAAAATCCAACAAATATATGAACATAACAATATTATAATATATGAATCGGTTATACTAACCCAAAACCCACAAACCCCTTTAACAAATTCAAACCCACCTCTCTGTCTCACTCTCACTCTCTCTCTCCACAACATTTCCACTTCCATGGAAGAAACACTACCAATGTTAACCACTCGCCGGACTGACCAAAGCCCCGTCAACCACCGTGACCACCTCCCCAAAAGCCCAAGAGTTCCAATGGAGTTTCTCTCAAGGTCATGGAGTGCCTCAGCTCTTGAAGTCTCCAAAGCTCTGTCTCAGTCTCACCCTCCTCCGCTGCCTCCTCCTCCTCCTTCATGCATGGCTAATTCCAAGTCCTCAAACAGCTCTTCCTCCTGCACCACCACCGCAACCACTACCACTCCTTCCATTCCTGAAGATATCGCAGGAGAAGCAGAGGAGCTGCCGTCCCTCCCCGGAAACCAATTTTACTTTGCTTCCTCTGTCACTTCCCAACTTGTCCTTGACCGCATTATGTCACAGTCCATGAGAGAGGAAGTGTCTCCATTGACATCAGGGAGGCTCTCACACAGCAGCGGACCTCTGAACAATGGTGGTTCTCTAACAGAGACAGATAGCCCTCCAGTCTCACCCTCCGATGAGTTCGACGACGTCGTTAAGTATTTTCGAACCCACAACAGCATACAAAACCTGTTCAACGGCAGCCGGAGCAGCGCTGGAACTGGCAACAATGCCCCTAGCGGCGGAGCCAAGACGGTGGGGAGGTGGCTGAAAGAGAGAAAAGAGAAGAAAAAGGAGGAGACGAGAGTGCACAATGCGCAGGTCCACGCGGCGGTTTCAGTTGCTGCGGTGGCTGCTGCAGTGGCCGCCATTGCTGCAGCCACAGCGGCTTCTTCGGGGTCGAGAAAAAACGATCAAGCAGGGAAGACTGACATGGCAGTGGCGTCTGCCGCCACGTTGGTAGCTGCTCAATGTGCCGAGGCTGCGGAAGCTATGGGAGCTGAGCATGACCATCTTACTTCAGTGGTCAGCTCCGCCGTTAATGTTCATTCACATGATGATATTACCACCCTCACTGCAGCTGCTGCGACAGCTTTACGAGGGGCAGCGACCCTAAAGGCAAGGGCAATGAAGGAGGTGTGGAACATATCAGCAGTGATTCCAGTGGAAAAGGGAATGGGAATCGGAGTCTGCGGTGGTAAAACTAACAACAACGGTGGTGATCGCAGCAGCGGCAGCAGCTTTAGTGATCGATCTTGTCCCTGTTGA

Protein Analysis

426

Amino Acids

44.63

Weight (kDa)

6.5

Isoelectric Point (pI)

56.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_canalis PF05703 76 - 395 2.1e-110 Auxin canalisation
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 44
AatII GACGTC 1 cut(s) 663
Acc16I TGCGCA 1 cut(s) 828
Acc36I ACCTGC 1 cut(s) 820
AccII CGCG 1 cut(s) 839
AcoI YGGCCR 1 cut(s) 873
AcsI RAATTY 1 cut(s) 94
AcuI CTGAAG 2 cut(s) 444, 1014
AcyI GRCGYC 2 cut(s) 660, 953
AfeI AGCGCT 1 cut(s) 724
AfiI CCNNNNNNNGG 3 cut(s) 186, 752, 841
AgsI TTSAA 3 cut(s) 99, 287, 706
Alw21I GWGCWC 1 cut(s) 822
Alw26I GTCTC 8 cut(s) 119, 295, 309, 315, 555, 611, 640, 803
Alw44I GTGCAC 1 cut(s) 818
AlwNI CAGNNNCTG 2 cut(s) 854, 1094
Aor51HI AGCGCT 1 cut(s) 724
AoxI GGCC 1 cut(s) 873
ApaLI GTGCAC 1 cut(s) 818
ApoI RAATTY 1 cut(s) 94
AspLEI GCGC 2 cut(s) 725, 829
AspS9I GGNCC 2 cut(s) 587, 832
AsuC2I CCSGG 1 cut(s) 465
AsuHPI GGTGA 3 cut(s) 305, 630, 1241
AsuII TTCGAA 1 cut(s) 676
AvaII GGWCC 2 cut(s) 587, 832
BaeGI GKGCMC 1 cut(s) 822
BarI GAAGNNNNNNTAC 2 cut(s) 150, 182
BbsI GAAGAC 1 cut(s) 941
Bbv12I GWGCWC 1 cut(s) 822
BbvCI CCTCAGC 1 cut(s) 277
BccI CCATC 1 cut(s) 1029
BceAI ACGGC 3 cut(s) 439, 724, 1031
BcnI CCSGG 1 cut(s) 465
BcoDI GTCTC 8 cut(s) 119, 295, 309, 315, 555, 611, 640, 803
BfaI CTAG 1 cut(s) 747
BfmI CTRYAG 3 cut(s) 867, 885, 1086
BfoI RGCGCY 1 cut(s) 726
BfuAI ACCTGC 1 cut(s) 820
BglI GCCNNNNNGGC 1 cut(s) 896
BlpI GCTNAGC 1 cut(s) 1011
Bme1390I CCNGG 1 cut(s) 465
Bme18I GGWCC 2 cut(s) 587, 832
BmgT120I GGNCC 2 cut(s) 587, 832
BmiI GGNNCC 1 cut(s) 757
BmrFI CCNGG 1 cut(s) 465
BpiI GAAGAC 1 cut(s) 941
BplI GAGNNNNNCTC 2 cut(s) 242, 274
BpmI CTGGAG 1 cut(s) 615
Bpu10I CCTNAGC 1 cut(s) 277
Bpu1102I GCTNAGC 1 cut(s) 1011
Bpu14I TTCGAA 1 cut(s) 676
BpuEI CTTGAG 1 cut(s) 244
BpuMI CCSGG 1 cut(s) 465
Bsa29I ATCGAT 1 cut(s) 1261
BsaHI GRCGYC 2 cut(s) 660, 953
BsaJI CCNNGG 3 cut(s) 153, 463, 987
BsaXI ACNNNNNCTCC 4 cut(s) 394, 424, 440, 470
Bsc4I CCNNNNNNNGG 3 cut(s) 186, 752, 841
Bse1I ACTGG 3 cut(s) 632, 736, 1172
Bse3DI GCAATG 2 cut(s) 879, 1143
BseCI ATCGAT 1 cut(s) 1261
BseDI CCNNGG 3 cut(s) 153, 463, 987
BseLI CCNNNNNNNGG 3 cut(s) 186, 752, 841
BseMI GCAATG 2 cut(s) 879, 1143
BseMII CTCAG 3 cut(s) 291, 320, 1002
BseNI ACTGG 3 cut(s) 632, 736, 1172
BseRI GAGGAG 6 cut(s) 309, 321, 324, 327, 461, 821
BseSI GKGCMC 1 cut(s) 822
BsgI GTGCAG 1 cut(s) 370
Bsh1236I CGCG 1 cut(s) 839
BshFI GGCC 1 cut(s) 875
BshVI ATCGAT 1 cut(s) 1261
BsiHKAI GWGCWC 1 cut(s) 822
BsiSI CCGG 3 cut(s) 187, 465, 715
BslFI GGGAC 2 cut(s) 442, 1254
BslI CCNNNNNNNGG 3 cut(s) 186, 752, 841
BsmAI GTCTC 8 cut(s) 119, 295, 309, 315, 555, 611, 640, 803
BsmBI CGTCTC 1 cut(s) 803
BsmFI GGGAC 2 cut(s) 442, 1254
BsnI GGCC 1 cut(s) 875
Bsp119I TTCGAA 1 cut(s) 676
Bsp1286I GDGCHC 1 cut(s) 822
Bsp143I GATC 4 cut(s) 922, 1231, 1258, 1262
Bsp1720I GCTNAGC 1 cut(s) 1011
Bsp19I CCATGG 1 cut(s) 153
BspANI GGCC 1 cut(s) 875
BspCNI CTCAG 3 cut(s) 290, 319, 1003
BspDI ATCGAT 1 cut(s) 1261
BspFNI CGCG 1 cut(s) 839
BspLI GGNNCC 1 cut(s) 757
BspMAI CTGCAG 3 cut(s) 871, 889, 1090
BspMI ACCTGC 1 cut(s) 820
BspQI GCTCTTC 1 cut(s) 382
BspT104I TTCGAA 1 cut(s) 676
BsrDI GCAATG 2 cut(s) 879, 1143
BsrI ACTGG 3 cut(s) 632, 736, 1172
BssECI CCNNGG 3 cut(s) 153, 463, 987
BssMI GATC 4 cut(s) 922, 1231, 1258, 1262
BssNI GRCGYC 2 cut(s) 660, 953
BssT1I CCWWGG 1 cut(s) 153
Bst4CI ACNGT 4 cut(s) 215, 531, 766, 1226
Bst6I CTCTTC 1 cut(s) 382
BstACI GRCGYC 2 cut(s) 660, 953
BstAPI GCANNNNNTGC 1 cut(s) 1094
BstBI TTCGAA 1 cut(s) 676
BstDEI CTNAG 3 cut(s) 277, 306, 1011
BstDSI CCRYGG 1 cut(s) 153
BstFNI CGCG 1 cut(s) 839
BstH2I RGCGCY 1 cut(s) 726
BstHHI GCGC 2 cut(s) 725, 829
BstKTI GATC 4 cut(s) 925, 1234, 1261, 1265
BstMAI GTCTC 8 cut(s) 119, 295, 309, 315, 555, 611, 640, 803
BstMBI GATC 4 cut(s) 922, 1231, 1258, 1262
BstSCI CCNGG 1 cut(s) 463
BstSFI CTRYAG 3 cut(s) 867, 885, 1086
BstSLI GKGCMC 1 cut(s) 822
BstUI CGCG 1 cut(s) 839
BstV2I GAAGAC 1 cut(s) 941
BstXI CCANNNNNNTGG 2 cut(s) 154, 766
Bsu15I ATCGAT 1 cut(s) 1261
BsuRI GGCC 1 cut(s) 875
BsuTUI ATCGAT 1 cut(s) 1261
BtgI CCRYGG 1 cut(s) 153
BtsI GCAGTG 4 cut(s) 876, 954, 1083, 1170
BtsIMutI CAGTG 6 cut(s) 876, 954, 1038, 1083, 1170, 1179
BveI ACCTGC 1 cut(s) 820
CaiI CAGNNNCTG 2 cut(s) 854, 1094
CfoI GCGC 2 cut(s) 725, 829
Cfr13I GGNCC 2 cut(s) 587, 832
ClaI ATCGAT 1 cut(s) 1261
CseI GACGC 1 cut(s) 942
CspCI CAANNNNNGTGG 2 cut(s) 1207, 1242
CviAII CATG 8 cut(s) 154, 267, 345, 349, 535, 943, 1016, 1064
DdeI CTNAG 3 cut(s) 277, 306, 1011
DpnI GATC 4 cut(s) 924, 1233, 1260, 1264
DpnII GATC 4 cut(s) 922, 1231, 1258, 1262
EaeI YGGCCR 1 cut(s) 873
Eam1104I CTCTTC 1 cut(s) 382
EarI CTCTTC 1 cut(s) 382
EciI GGCGGA 2 cut(s) 768, 1033
Eco130I CCWWGG 1 cut(s) 153
Eco32I GATATC 1 cut(s) 430
Eco47I GGWCC 2 cut(s) 587, 832
Eco47III AGCGCT 1 cut(s) 724
Eco57I CTGAAG 2 cut(s) 444, 1014
EcoRV GATATC 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 153
EcoT22I ATGCAT 1 cut(s) 350
ErhI CCWWGG 1 cut(s) 153
Esp3I CGTCTC 1 cut(s) 803
FaeI CATG 8 cut(s) 157, 270, 348, 352, 538, 946, 1019, 1067
FaqI GGGAC 2 cut(s) 442, 1254
FatI CATG 8 cut(s) 153, 266, 344, 348, 534, 942, 1015, 1063
FspBI CTAG 1 cut(s) 747
FspI TGCGCA 1 cut(s) 828
GlaI GCGC 2 cut(s) 724, 828
GsuI CTGGAG 1 cut(s) 615
HaeII RGCGCY 1 cut(s) 726
HaeIII GGCC 1 cut(s) 875
HapII CCGG 3 cut(s) 187, 465, 715
HgaI GACGC 1 cut(s) 942
HhaI GCGC 2 cut(s) 725, 829
Hin1I GRCGYC 2 cut(s) 660, 953
Hin1II CATG 8 cut(s) 157, 270, 348, 352, 538, 946, 1019, 1067
Hin6I GCGC 2 cut(s) 723, 827
HinP1I GCGC 2 cut(s) 723, 827
HincII GTYRAC 2 cut(s) 177, 208
HindII GTYRAC 2 cut(s) 177, 208
HinfI GANTC 4 cut(s) 53, 1168, 1191, 1197
HpaI GTTAAC 1 cut(s) 177
HpaII CCGG 3 cut(s) 187, 465, 715
HphI GGTGA 3 cut(s) 305, 630, 1241
Hpy166II GTNNAC 4 cut(s) 177, 208, 820, 835
Hpy188I TCNGA 3 cut(s) 594, 646, 1196
Hpy188III TCNNGA 3 cut(s) 284, 422, 912
Hpy8I GTNNAC 4 cut(s) 177, 208, 820, 835
Hpy99I CGWCG 3 cut(s) 659, 662, 665
HpyAV CCTTC 3 cut(s) 351, 423, 1135
HpyCH4III ACNGT 4 cut(s) 215, 531, 766, 1226
HpyCH4IV ACGT 2 cut(s) 660, 965
HpyCH4V TGCA 6 cut(s) 348, 387, 820, 869, 887, 1088
HpyF3I CTNAG 3 cut(s) 277, 306, 1011
HpySE526I ACGT 2 cut(s) 660, 965
Hsp92I GRCGYC 2 cut(s) 660, 953
Hsp92II CATG 8 cut(s) 157, 270, 348, 352, 538, 946, 1019, 1067
HspAI GCGC 2 cut(s) 723, 827
KspAI GTTAAC 1 cut(s) 177
Kzo9I GATC 4 cut(s) 922, 1231, 1258, 1262
LguI GCTCTTC 1 cut(s) 382
LmnI GCTCC 5 cut(s) 448, 717, 755, 1007, 1046
MaeI CTAG 1 cut(s) 747
MaeII ACGT 2 cut(s) 660, 965
MaeIII GTNAC 3 cut(s) 215, 493, 525
MalI GATC 4 cut(s) 924, 1233, 1260, 1264
MboI GATC 4 cut(s) 922, 1231, 1258, 1262
MboII GAAGA 6 cut(s) 170, 369, 437, 808, 894, 946
MhlI GDGCHC 1 cut(s) 822
MluCI AATT 3 cut(s) 94, 355, 473
MlyI GAGTC 1 cut(s) 1206
MmeI TCCRAC 1 cut(s) 40
Mph1103I ATGCAT 1 cut(s) 350
MseI TTAA 4 cut(s) 89, 176, 666, 1050
MslI CAYNNNNRTG 3 cut(s) 152, 947, 1062
MspA1I CMGCKG 5 cut(s) 325, 585, 896, 1091, 1242
MspI CCGG 3 cut(s) 187, 465, 715
MspR9I CCNGG 1 cut(s) 465
MvnI CGCG 1 cut(s) 839
NciI CCSGG 1 cut(s) 465
NcoI CCATGG 1 cut(s) 153
NdeII GATC 4 cut(s) 922, 1231, 1258, 1262
NlaIII CATG 8 cut(s) 157, 270, 348, 352, 538, 946, 1019, 1067
NlaIV GGNNCC 1 cut(s) 757
NmeAIII GCCGAG 1 cut(s) 1012
NmuCI GTSAC 3 cut(s) 215, 493, 525
NsbI TGCGCA 1 cut(s) 828
NsiI ATGCAT 1 cut(s) 350
NspV TTCGAA 1 cut(s) 676
PciSI GCTCTTC 1 cut(s) 382
PcsI WCGNNNNNNNCGW 1 cut(s) 660
PfeI GAWTC 3 cut(s) 53, 1168, 1191
PflFI GACNNNGTC 1 cut(s) 659
PleI GAGTC 1 cut(s) 1205
PpsI GAGTC 1 cut(s) 1205
PsiI TTATAA 1 cut(s) 44
PspN4I GGNNCC 1 cut(s) 757
PspPI GGNCC 2 cut(s) 587, 832
PstI CTGCAG 3 cut(s) 871, 889, 1090
PstNI CAGNNNCTG 2 cut(s) 854, 1094
PsyI GACNNNGTC 1 cut(s) 659
PvuII CAGCTG 1 cut(s) 1091
RseI CAYNNNNRTG 3 cut(s) 152, 947, 1062
SapI GCTCTTC 1 cut(s) 382
SaqAI TTAA 4 cut(s) 89, 176, 666, 1050
Sau3AI GATC 4 cut(s) 922, 1231, 1258, 1262
Sau96I GGNCC 2 cut(s) 587, 832
SchI GAGTC 1 cut(s) 1206
ScrFI CCNGG 1 cut(s) 465
SduI GDGCHC 1 cut(s) 822
SfcI CTRYAG 3 cut(s) 867, 885, 1086
SfuI TTCGAA 1 cut(s) 676
SinI GGWCC 2 cut(s) 587, 832
SmiMI CAYNNNNRTG 3 cut(s) 152, 947, 1062
SmlI CTYRAG 1 cut(s) 259
SmoI CTYRAG 1 cut(s) 259
Sse9I AATT 3 cut(s) 94, 355, 473
SspI AATATT 1 cut(s) 40
SspMI CTAG 1 cut(s) 747
StyD4I CCNGG 1 cut(s) 463
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 4 cut(s) 215, 531, 766, 1226
TaiI ACGT 2 cut(s) 663, 968
TaqI TCGA 4 cut(s) 654, 676, 911, 1261
TasI AATT 3 cut(s) 94, 355, 473
TauI GCSGC 6 cut(s) 753, 842, 878, 899, 962, 1245
TfiI GAWTC 3 cut(s) 53, 1168, 1191
Tru1I TTAA 4 cut(s) 89, 176, 666, 1050
Tru9I TTAA 4 cut(s) 89, 176, 666, 1050
TscAI CASTG 6 cut(s) 876, 954, 1038, 1090, 1170, 1179
TseFI GTSAC 3 cut(s) 215, 493, 525
Tsp45I GTSAC 3 cut(s) 215, 493, 525
TspDTI ATGAA 5 cut(s) 42, 66, 333, 1046, 1154
TspRI CASTG 6 cut(s) 876, 954, 1038, 1090, 1170, 1179
Tth111I GACNNNGTC 1 cut(s) 659
VneI GTGCAC 1 cut(s) 818
VpaK11BI GGWCC 2 cut(s) 587, 832
XapI RAATTY 1 cut(s) 94
XspI CTAG 1 cut(s) 747
ZraI GACGTC 1 cut(s) 661
Zsp2I ATGCAT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.