pycom11g26390

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0005712
Physical Location & Seq
Reverse (-)
28957872 .. 28959845
1974 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 810 bp
ATGGGGGTCGTCACCATTGGTGAACTGAAGCCTAGCATTTCCGGGAAGCGGTCTATTCGTCCGAGTTCAAGCATAAGGCATGCCACTGAATGGGAAAATACAGAGGTTCCTTGCAGGGTTTTTGATATGTTGGCACACATGAGAATGCCAATCTCCGATGTTTCTAGCGATCTTACCGTGGAGATAGGAGCATCAAGCTTTGCACTTCACAAGTTCCCTCTAGTTTCTCGGAGTGGAAAGATTCGGAAGCTGTTGCTTGAAGCAAAAGATTCAAAAGTTTCGCGCATAATTCTTGCATCTGTTCCGGGTGGACCAGAGGGATTTGAGCTAGCTGCGAAATTCTGTTACGGAGTTAATGTTGAGATTACGCAATCAAATGTTGCCACACTTCGTTGCGTAGCGCACTTTCTGGAAATGACGGAAGAGTATGCTGAGAAAAACATGGAAACCAGAACTGAAGCTTATCTGAAGGAGACGGTTTTCCCAAACATATCAAAGTCGATATCAGTTCTTCATCGCTGCGAAACCCTCTTGCCGGCAGCAGAAGAAACCAACTTGGTAAGCAGGCTTATCAATGCAATCGCAAACAATGTGTGCAAGGAGCAGCTCACCTCTGGCTTATTGAAACTTGACCACAACTTTCCTGCGAAAGCGGCTTTAAATATGCAACCTGAAACGCCTGCAGACTGGTGGGGAAAAGCGCTTACAGTGCTTAGTCTCGACTTCTTCAAACGTGTTATATCCACGGTGAAAACCAAGGGTCTTAAACAGGATATGATCGATCAAAATTTTGATCAACTATGCTCATAA

Protein Analysis

270

Amino Acids

29.84

Weight (kDa)

7.63

Isoelectric Point (pI)

40.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 56 - 141 1.9e-06 BTB/POZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 90
AccII CGCG 1 cut(s) 283
AciI CCGC 2 cut(s) 49, 653
AcsI RAATTY 2 cut(s) 338, 787
AcuI CTGAAG 3 cut(s) 47, 477, 488
AfeI AGCGCT 1 cut(s) 702
AfiI CCNNNNNNNGG 3 cut(s) 48, 90, 535
AflIII ACRYGT 1 cut(s) 733
AgsI TTSAA 5 cut(s) 69, 260, 273, 625, 730
AluBI AGCT 6 cut(s) 198, 250, 328, 332, 461, 607
AluI AGCT 6 cut(s) 198, 250, 328, 332, 461, 607
Alw26I GTCTC 2 cut(s) 467, 722
Aor51HI AGCGCT 1 cut(s) 702
ApeKI GCWGC 4 cut(s) 332, 519, 539, 604
ApoI RAATTY 2 cut(s) 338, 787
AspLEI GCGC 3 cut(s) 285, 403, 703
AspS9I GGNCC 1 cut(s) 311
AsuC2I CCSGG 2 cut(s) 43, 306
AsuHPI GGTGA 4 cut(s) 4, 32, 601, 760
AsuNHI GCTAGC 1 cut(s) 328
AvaII GGWCC 1 cut(s) 311
BbvI GCAGC 4 cut(s) 319, 506, 551, 616
BclI TGATCA 1 cut(s) 793
BcnI CCSGG 2 cut(s) 43, 306
BcoDI GTCTC 2 cut(s) 467, 722
BfaI CTAG 4 cut(s) 33, 165, 221, 329
BfmI CTRYAG 1 cut(s) 681
BfoI RGCGCY 1 cut(s) 704
BisI GCNGC 5 cut(s) 333, 520, 540, 605, 654
BlsI GCNGC 5 cut(s) 334, 521, 541, 606, 655
Bme1390I CCNGG 2 cut(s) 43, 306
Bme18I GGWCC 1 cut(s) 311
BmgT120I GGNCC 1 cut(s) 311
BmiI GGNNCC 1 cut(s) 108
BmrFI CCNGG 2 cut(s) 43, 306
BmsI GCATC 2 cut(s) 200, 305
BmtI GCTAGC 1 cut(s) 332
BpuMI CCSGG 2 cut(s) 43, 306
Bsa29I ATCGAT 1 cut(s) 780
BsaJI CCNNGG 3 cut(s) 177, 744, 756
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 90, 535
Bse118I RCCGGY 1 cut(s) 535
Bse1I ACTGG 1 cut(s) 692
BseCI ATCGAT 1 cut(s) 780
BseDI CCNNGG 3 cut(s) 177, 744, 756
BseLI CCNNNNNNNGG 3 cut(s) 48, 90, 535
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 1 cut(s) 692
BseXI GCAGC 4 cut(s) 319, 506, 551, 616
Bsh1236I CGCG 1 cut(s) 283
BshVI ATCGAT 1 cut(s) 780
BsiSI CCGG 3 cut(s) 42, 305, 536
BslI CCNNNNNNNGG 3 cut(s) 48, 90, 535
BsmAI GTCTC 2 cut(s) 467, 722
BsmBI CGTCTC 1 cut(s) 467
BsmI GAATGC 1 cut(s) 150
Bsp143I GATC 4 cut(s) 169, 777, 781, 793
BspACI CCGC 2 cut(s) 49, 653
BspCNI CTCAG 1 cut(s) 424
BspDI ATCGAT 1 cut(s) 780
BspFNI CGCG 1 cut(s) 283
BspLI GGNNCC 1 cut(s) 108
BspMAI CTGCAG 1 cut(s) 685
BspOI GCTAGC 1 cut(s) 332
BsrFI RCCGGY 1 cut(s) 535
BsrI ACTGG 1 cut(s) 692
BssAI RCCGGY 1 cut(s) 535
BssECI CCNNGG 3 cut(s) 177, 744, 756
BssMI GATC 4 cut(s) 169, 777, 781, 793
BssT1I CCWWGG 1 cut(s) 756
Bst4CI ACNGT 4 cut(s) 178, 478, 709, 748
Bst6I CTCTTC 1 cut(s) 417
BstC8I GCNNGC 5 cut(s) 81, 330, 537, 566, 681
BstDEI CTNAG 2 cut(s) 432, 713
BstDSI CCRYGG 2 cut(s) 177, 744
BstFNI CGCG 1 cut(s) 283
BstH2I RGCGCY 1 cut(s) 704
BstHHI GCGC 3 cut(s) 285, 403, 703
BstKTI GATC 4 cut(s) 172, 780, 784, 796
BstMAI GTCTC 2 cut(s) 467, 722
BstMBI GATC 4 cut(s) 169, 777, 781, 793
BstMWI GCNNNNNNNGC 2 cut(s) 653, 709
BstNSI RCATGY 1 cut(s) 83
BstSCI CCNGG 2 cut(s) 41, 304
BstSFI CTRYAG 1 cut(s) 681
BstUI CGCG 1 cut(s) 283
BstV1I GCAGC 4 cut(s) 319, 506, 551, 616
Bsu15I ATCGAT 1 cut(s) 780
BsuTUI ATCGAT 1 cut(s) 780
BtgI CCRYGG 2 cut(s) 177, 744
BtgZI GCGATG 1 cut(s) 500
BtsIMutI CAGTG 2 cut(s) 84, 714
Cac8I GCNNGC 5 cut(s) 81, 330, 537, 566, 681
CfoI GCGC 3 cut(s) 285, 403, 703
Cfr10I RCCGGY 1 cut(s) 535
Cfr13I GGNCC 1 cut(s) 311
ClaI ATCGAT 1 cut(s) 780
CviAII CATG 3 cut(s) 80, 139, 442
DdeI CTNAG 2 cut(s) 432, 713
DpnI GATC 4 cut(s) 171, 779, 783, 795
DpnII GATC 4 cut(s) 169, 777, 781, 793
DraI TTTAAA 1 cut(s) 660
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
Eco130I CCWWGG 1 cut(s) 756
Eco32I GATATC 1 cut(s) 504
Eco47I GGWCC 1 cut(s) 311
Eco47III AGCGCT 1 cut(s) 702
Eco57I CTGAAG 3 cut(s) 47, 477, 488
EcoRV GATATC 1 cut(s) 504
EcoT14I CCWWGG 1 cut(s) 756
ErhI CCWWGG 1 cut(s) 756
Esp3I CGTCTC 1 cut(s) 467
FaeI CATG 3 cut(s) 83, 142, 445
FatI CATG 3 cut(s) 79, 138, 441
FbaI TGATCA 1 cut(s) 793
Fnu4HI GCNGC 5 cut(s) 333, 520, 540, 605, 654
Fsp4HI GCNGC 5 cut(s) 333, 520, 540, 605, 654
FspBI CTAG 4 cut(s) 33, 165, 221, 329
GlaI GCGC 3 cut(s) 284, 402, 702
GluI GCNGC 5 cut(s) 333, 520, 540, 605, 654
HaeII RGCGCY 1 cut(s) 704
HapII CCGG 3 cut(s) 42, 305, 536
HhaI GCGC 3 cut(s) 285, 403, 703
Hin1II CATG 3 cut(s) 83, 142, 445
Hin6I GCGC 3 cut(s) 283, 401, 701
HinP1I GCGC 3 cut(s) 283, 401, 701
HindIII AAGCTT 2 cut(s) 196, 459
HinfI GANTC 2 cut(s) 241, 269
HpaII CCGG 3 cut(s) 42, 305, 536
HphI GGTGA 4 cut(s) 4, 32, 601, 760
Hpy166II GTNNAC 2 cut(s) 23, 311
Hpy188I TCNGA 5 cut(s) 63, 157, 231, 246, 468
Hpy188III TCNNGA 2 cut(s) 410, 719
Hpy8I GTNNAC 2 cut(s) 23, 311
HpyAV CCTTC 1 cut(s) 463
HpyCH4III ACNGT 4 cut(s) 178, 478, 709, 748
HpyCH4IV ACGT 1 cut(s) 733
HpyCH4V TGCA 7 cut(s) 114, 203, 296, 578, 597, 667, 683
HpyF10VI GCNNNNNNNGC 2 cut(s) 653, 709
HpyF3I CTNAG 2 cut(s) 432, 713
HpySE526I ACGT 1 cut(s) 733
Hsp92II CATG 3 cut(s) 83, 142, 445
HspAI GCGC 3 cut(s) 283, 401, 701
KroI GCCGGC 1 cut(s) 535
KroNI GCCGGC 1 cut(s) 537
Ksp22I TGATCA 1 cut(s) 793
Kzo9I GATC 4 cut(s) 169, 777, 781, 793
LmnI GCTCC 2 cut(s) 188, 601
Lsp1109I GCAGC 4 cut(s) 319, 506, 551, 616
LweI GCATC 2 cut(s) 200, 305
MaeI CTAG 4 cut(s) 33, 165, 221, 329
MaeII ACGT 1 cut(s) 733
MaeIII GTNAC 2 cut(s) 10, 344
MalI GATC 4 cut(s) 171, 779, 783, 795
MboI GATC 4 cut(s) 169, 777, 781, 793
MboII GAAGA 4 cut(s) 434, 503, 557, 718
MluCI AATT 3 cut(s) 288, 338, 787
MnlI CCTC 5 cut(s) 97, 228, 310, 539, 622
MroNI GCCGGC 1 cut(s) 535
MseI TTAA 3 cut(s) 354, 659, 765
MslI CAYNNNNRTG 1 cut(s) 143
MspI CCGG 3 cut(s) 42, 305, 536
MspR9I CCNGG 2 cut(s) 43, 306
Mva1269I GAATGC 1 cut(s) 150
MvnI CGCG 1 cut(s) 283
MwoI GCNNNNNNNGC 2 cut(s) 653, 709
NaeI GCCGGC 1 cut(s) 537
NciI CCSGG 2 cut(s) 43, 306
NdeII GATC 4 cut(s) 169, 777, 781, 793
NgoMIV GCCGGC 1 cut(s) 535
NheI GCTAGC 1 cut(s) 328
NlaIII CATG 3 cut(s) 83, 142, 445
NlaIV GGNNCC 1 cut(s) 108
NmuCI GTSAC 1 cut(s) 10
NspI RCATGY 1 cut(s) 83
PaeI GCATGC 1 cut(s) 83
PctI GAATGC 1 cut(s) 150
PdiI GCCGGC 1 cut(s) 537
PfeI GAWTC 2 cut(s) 241, 269
PflMI CCANNNNNTGG 1 cut(s) 90
PfoI TCCNGGA 1 cut(s) 41
PkrI GCNGC 5 cut(s) 334, 521, 541, 606, 655
PspN4I GGNNCC 1 cut(s) 108
PspPI GGNCC 1 cut(s) 311
PstI CTGCAG 1 cut(s) 685
RseI CAYNNNNRTG 1 cut(s) 143
SaqAI TTAA 3 cut(s) 354, 659, 765
SatI GCNGC 5 cut(s) 333, 520, 540, 605, 654
Sau3AI GATC 4 cut(s) 169, 777, 781, 793
Sau96I GGNCC 1 cut(s) 311
ScrFI CCNGG 2 cut(s) 43, 306
SfaNI GCATC 2 cut(s) 200, 305
SfcI CTRYAG 1 cut(s) 681
SinI GGWCC 1 cut(s) 311
SmiMI CAYNNNNRTG 1 cut(s) 143
SphI GCATGC 1 cut(s) 83
Sse9I AATT 3 cut(s) 288, 338, 787
SsiI CCGC 2 cut(s) 49, 653
SspMI CTAG 4 cut(s) 33, 165, 221, 329
StyD4I CCNGG 2 cut(s) 41, 304
StyI CCWWGG 1 cut(s) 756
TaaI ACNGT 4 cut(s) 178, 478, 709, 748
TaiI ACGT 1 cut(s) 736
TaqI TCGA 3 cut(s) 500, 720, 780
TasI AATT 3 cut(s) 288, 338, 787
TauI GCSGC 1 cut(s) 656
TfiI GAWTC 2 cut(s) 241, 269
Tru1I TTAA 3 cut(s) 354, 659, 765
Tru9I TTAA 3 cut(s) 354, 659, 765
TscAI CASTG 2 cut(s) 91, 714
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 4 cut(s) 332, 519, 539, 604
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 1 cut(s) 503
TspGWI ACGGA 2 cut(s) 363, 434
TspRI CASTG 2 cut(s) 91, 714
Van91I CCANNNNNTGG 1 cut(s) 90
VpaK11BI GGWCC 1 cut(s) 311
XapI RAATTY 2 cut(s) 338, 787
XceI RCATGY 1 cut(s) 83
XspI CTAG 4 cut(s) 33, 165, 221, 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.