Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0009369

Physical Location & Seq

Genomic Coordinates Forward (+)

112652 .. 114430

Gene Structure 1779 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g00060.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1779 bp

ATGAGAAAAATGGCGAGAATCACATCACTGTACTCGAATTTGCTACGTAAAGACACGCATAGCTATGTCTCTGTCGTGCCCATTGCTGCTGCCTTTGCCCTGAAGCAATTCCCTGCTGCTGCTGTATCAGCACCTCCAACGCATTTCTGTACTCCCGTCCTCCCTTGGTCTTCTTCTTCGACTAGCTCACACTCCAAGTCTCCAAACTCAGCTGATGACGATTCCACAGCAGAAGAATCACATGTCAATGTTGCTGTTGCTGACGACGACGAAGATGATGATGATGATACTGAGTTGGGAGTCTCGGATTCGGGTCTTTCTCGAGATGTGAAAAGCATTGTCGACATCTTAAGTTTACGGAGCGAGTCGCTGGGCTCTGAGATGAAGAGGAAGCTCGATCAATGCGGCATAGCAGCCTCGTCAGAGCTGGTGGCAGCGGTTCTTTCACGCGTTCGCAATGATTGGGAATCGGCATTCACCTTCTTCCTCTGGGCCGGAAAGCAGCAGCCGGGTTACTCTCACTCGGTGCGGGAATACCATTCCATGATCTCCATTCTAGGGAAAATGAGAAAGTTTGACACTGCCTGGGCCTTGATTGATGAGATGAAAGGTCATTCTCTCGTCACTCCTCACACTCTCTTGATCATGATCAGGAGGTACTCTTGCGTTCATGATGTTGGCAAGGCTATCAATACATTCTATGCTTATAAACGCTTTCACTTTGTGGCTGCCATGGATGACTTCCATGGCCTTCTCTCTGCCCTTTGCCGCTACAAAAATGTCCAAGAAGCTGAGCACTTGCTCTTCTGCAACAGAAACGCCTTCCCCTTTAACACCAAGAGCTTTAACATTATCCTCAATGGTTGGTGCAATATCATCGTCAGTCCCCGTGAGGCCGAGAGAATTTGGATGGAGATGACCAACAGAGGTGTTGTGCACGATGCTGTCTCCTACTCATGTCTCATGTCTTGCTATTCAAAAGCCTCTAACCTTAACAGGGTGCTCAAGCTCTTCGATCGGATGAAGGCAATGAAGATTGAACCCGATAGGAAAGTTTACAATGCGCTCATTTATGCTCTTGGAAAAGGCAGACTTCTGAATGAGGCTATCAATTTAGTGAAAACAATGCAAGATAAAGGCCTTACTCCCAATGTTGTCACTTTTAACTCCATCATCAAGCCTCTCTGCAAGGCCAGGAAAATTGATGAGGCTAGACGCATACTTGATGACATGTTGCACCAAGGCCTTCTTCCCACAATTCAGACTTACCATGCTTTCTTCCGTATTTTAAGGACTGGTGAGCAAGTGTTTGAGCTCTTGGAGAAGATAAAAAGTTTGGGTTGCCACCCAAATACTGATACCTACATAATGTTGATTCGAAAATTTTGTCGATGGCGCCAGCTTGATCATGTGTTCAAGTTGTGGAGTGAAATGAGTGAGAATGGAGTCAATCCCGATCGTAGCTCATATATTGTGCTGATACATGGGCTATTTTTGAATGGAAAGCTCGAGGAAGCCTACAAATATTATATAGAGATGAAAAAGAAACAGTATTTGCCAGAACCAAGGACAGAAGAGATGCTTCAGGCATGGTTGTCCGGTAAGCAGATTTCCACATTCCATACAACAGATAAAGAAGATGATCAATTGGACTGGAGTCACCTGGACATGAACGCTAGAGTAACCCCCAAGAAATCTCATCAGGAAAAAGATTTCCTTCGGCAGCTGGAAGCTAGGAAAGTTGTAAGAGAGCGGGGCTTTTCTTTGTGGGAGCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

593

Amino Acids

67.88

Weight (kDa)

8.56

Isoelectric Point (pI)

39.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	301 - 362	2e-09	Pentatricopeptide repeat domain
PPR_long	PF17177	301 - 444	8.8e-12	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	306 - 396	1.2e-06	Fungal tetratrico peptide repeats
PPR_2	PF13041	314 - 361	1.5e-09	PPR repeat family
PPR_2	PF13041	352 - 397	1.1e-10	PPR repeat family
PPR_3	PF13812	372 - 425	2.2e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	379 - 411	1.1e-10	PPR repeat
PPR_2	PF13041	383 - 428	4.3e-11	PPR repeat family
PPR_long	PF17177	405 - 536	4.2e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	458 - 497	5.4e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009369)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15010
fragaria_vesca	FvH4_1g14610 FvH4_6g48380 FvH4_6g48380 FvH4_6g48380 FvH4_6g48380 FvH4_6g48380
malus_domestica	MD12G1000700.v1.1
prunus_persica	Prupe.7G001100_v2.0.a1 Prupe.7G001100_v2.0.a1 Prupe.7G001100_v2.0.a1
pyrus_communis	pycom12g00060
rosa_chinensis	RchiOBHm_Chr3g0497531
rosa_laevigata	RLG00000022455
rosa_multiflora	Rmu_sc0000093.1_g000015
rosa_roxburghii	Rroxscaffold_6G00387950
rosa_rugosa	Rorug03G0293400
rosa_samantha	Rh3AG337300 Rh3BG375700 Rh3CG371700 Rh3DG372200
rosa_wichuraiana	Rw0G018510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	708
AccB1I	GGYRCC	1 cut(s)	1395
AccBSI	CCGCTC	1 cut(s)	1753
AccI	GTMKAC	1 cut(s)	342
AccII	CGCG	1 cut(s)	450
AciI	CCGC	5 cut(s)	405, 437, 529, 769, 1753
AcsI	RAATTY	3 cut(s)	37, 903, 1382
AcuI	CTGAAG	2 cut(s)	122, 1568
AcyI	GRCGYC	1 cut(s)	1396
AdeI	CACNNNGTG	2 cut(s)	526, 724
AfaI	GTAC	3 cut(s)	32, 151, 659
AfiI	CCNNNNNNNGG	2 cut(s)	558, 997
AflII	CTTAAG	1 cut(s)	349
AflIII	ACRYGT	3 cut(s)	241, 448, 1230
AgsI	TTSAA	4 cut(s)	978, 1040, 1417, 1498
AjnI	CCWGG	3 cut(s)	584, 1193, 1662
AjuI	GAANNNNNNNTTGG	2 cut(s)	1233, 1265
Alw21I	GWGCWC	4 cut(s)	798, 939, 1005, 1317
Alw26I	GTCTC	5 cut(s)	73, 204, 307, 952, 965
Alw44I	GTGCAC	1 cut(s)	935
Ama87I	CYCGRG	2 cut(s)	321, 1508
AoxI	GGCC	7 cut(s)	492, 588, 748, 894, 1138, 1191, 1243
ApaLI	GTGCAC	1 cut(s)	935
ApoI	RAATTY	3 cut(s)	37, 903, 1382
ArsI	GACNNNNNNTTYG	2 cut(s)	1372, 1404
Asp700I	GAANNNNTTC	1 cut(s)	107
AspLEI	GCGC	2 cut(s)	1066, 1398
AspS9I	GGNCC	2 cut(s)	492, 588
AsuC2I	CCSGG	1 cut(s)	510
AsuHPI	GGTGA	3 cut(s)	469, 1310, 1652
AsuII	TTCGAA	1 cut(s)	1378
AvaI	CYCGRG	2 cut(s)	321, 1508
BaeGI	GKGCMC	2 cut(s)	81, 939
BanI	GGYRCC	1 cut(s)	1395
BanII	GRGCYC	2 cut(s)	377, 1317
BbsI	GAAGAC	1 cut(s)	162
Bbv12I	GWGCWC	4 cut(s)	798, 939, 1005, 1317
BccI	CCATC	3 cut(s)	904, 1178, 1386
BcgI	CGANNNNNNTGC	2 cut(s)	859, 893
BciT130I	CCWGG	3 cut(s)	586, 1195, 1664
BclI	TGATCA	4 cut(s)	642, 648, 1405, 1642
BcnI	CCSGG	1 cut(s)	510
BcoDI	GTCTC	5 cut(s)	73, 204, 307, 952, 965
BfaI	CTAG	5 cut(s)	183, 557, 1212, 1677, 1734
BfoI	RGCGCY	1 cut(s)	1399
BfrI	CTTAAG	1 cut(s)	349
BlpI	GCTNAGC	1 cut(s)	792
Bme1390I	CCNGG	4 cut(s)	510, 586, 1195, 1664
BmeT110I	CYCGRG	2 cut(s)	321, 1508
BmgT120I	GGNCC	2 cut(s)	492, 588
BmiI	GGNNCC	2 cut(s)	1397, 1773
BmrFI	CCNGG	4 cut(s)	510, 586, 1195, 1664
BmsI	GCATC	2 cut(s)	931, 1569
BoxI	GACNNNNGTC	1 cut(s)	1656
BpiI	GAAGAC	1 cut(s)	162
BpmI	CTGGAG	1 cut(s)	1675
Bpu1102I	GCTNAGC	1 cut(s)	792
Bpu14I	TTCGAA	1 cut(s)	1378
BpuEI	CTTGAG	1 cut(s)	989
BpuMI	CCSGG	1 cut(s)	510
BsaAI	YACGTR	1 cut(s)	47
BsaBI	GATNNNNATC	1 cut(s)	647
BsaHI	GRCGYC	1 cut(s)	1396
BsaJI	CCNNGG	6 cut(s)	164, 585, 732, 745, 1240, 1565
BsaWI	WCCGGW	1 cut(s)	1598
Bsc4I	CCNNNNNNNGG	2 cut(s)	558, 997
Bse1I	ACTGG	2 cut(s)	1300, 1658
Bse3DI	GCAATG	3 cut(s)	81, 463, 1035
Bse8I	GATNNNNATC	1 cut(s)	647
BseBI	CCWGG	3 cut(s)	586, 1195, 1664
BseDI	CCNNGG	6 cut(s)	164, 585, 732, 745, 1240, 1565
BseGI	GGATG	3 cut(s)	742, 915, 1026
BseJI	GATNNNNATC	1 cut(s)	647
BseLI	CCNNNNNNNGG	2 cut(s)	558, 997
BseMI	GCAATG	3 cut(s)	81, 463, 1035
BseMII	CTCAG	4 cut(s)	222, 282, 369, 783
BseNI	ACTGG	2 cut(s)	1300, 1658
BseRI	GAGGAG	1 cut(s)	618
BseSI	GKGCMC	2 cut(s)	81, 939
BseYI	CCCAGC	1 cut(s)	370
Bsh1236I	CGCG	1 cut(s)	450
Bsh1285I	CGRYCG	2 cut(s)	1018, 1459
BshFI	GGCC	7 cut(s)	494, 590, 750, 896, 1140, 1193, 1245
BshNI	GGYRCC	1 cut(s)	1395
BsiEI	CGRYCG	2 cut(s)	1018, 1459
BsiHKAI	GWGCWC	4 cut(s)	798, 939, 1005, 1317
BsiHKCI	CYCGRG	2 cut(s)	321, 1508
BsiSI	CCGG	3 cut(s)	495, 509, 1599
BslFI	GGGAC	1 cut(s)	870
BslI	CCNNNNNNNGG	2 cut(s)	558, 997
BsmAI	GTCTC	5 cut(s)	73, 204, 307, 952, 965
BsmFI	GGGAC	1 cut(s)	870
BsmI	GAATGC	1 cut(s)	473
BsnI	GGCC	7 cut(s)	494, 590, 750, 896, 1140, 1193, 1245
BsoBI	CYCGRG	2 cut(s)	321, 1508
Bsp119I	TTCGAA	1 cut(s)	1378
Bsp1286I	GDGCHC	6 cut(s)	81, 377, 798, 939, 1005, 1317
Bsp143I	GATC	8 cut(s)	397, 546, 642, 648, 1015, 1405, 1456, 1642
Bsp1720I	GCTNAGC	1 cut(s)	792
Bsp19I	CCATGG	2 cut(s)	732, 745
BspACI	CCGC	5 cut(s)	405, 437, 529, 769, 1753
BspANI	GGCC	7 cut(s)	494, 590, 750, 896, 1140, 1193, 1245
BspCNI	CTCAG	4 cut(s)	221, 283, 370, 784
BspFNI	CGCG	1 cut(s)	450
BspHI	TCATGA	2 cut(s)	645, 670
BspLI	GGNNCC	2 cut(s)	1397, 1773
BspQI	GCTCTTC	2 cut(s)	809, 1016
BspT104I	TTCGAA	1 cut(s)	1378
BspT107I	GGYRCC	1 cut(s)	1395
BspTI	CTTAAG	1 cut(s)	349
BsrBI	CCGCTC	1 cut(s)	1753
BsrDI	GCAATG	3 cut(s)	81, 463, 1035
BsrI	ACTGG	2 cut(s)	1300, 1658
BssECI	CCNNGG	6 cut(s)	164, 585, 732, 745, 1240, 1565
BssMI	GATC	8 cut(s)	397, 546, 642, 648, 1015, 1405, 1456, 1642
BssNI	GRCGYC	1 cut(s)	1396
BssT1I	CCWWGG	5 cut(s)	164, 732, 745, 1240, 1565
Bst2UI	CCWGG	3 cut(s)	586, 1195, 1664
Bst4CI	ACNGT	2 cut(s)	30, 1551
Bst6I	CTCTTC	4 cut(s)	380, 809, 1016, 1569
BstACI	GRCGYC	1 cut(s)	1396
BstAFI	CTTAAG	1 cut(s)	349
BstBAI	YACGTR	1 cut(s)	47
BstBI	TTCGAA	1 cut(s)	1378
BstC8I	GCNNGC	1 cut(s)	1400
BstDEI	CTNAG	4 cut(s)	208, 291, 378, 792
BstDSI	CCRYGG	2 cut(s)	732, 745
BstF5I	GGATG	3 cut(s)	742, 915, 1026
BstFNI	CGCG	1 cut(s)	450
BstH2I	RGCGCY	1 cut(s)	1399
BstHHI	GCGC	2 cut(s)	1066, 1398
BstKTI	GATC	8 cut(s)	400, 549, 645, 651, 1018, 1408, 1459, 1645
BstMAI	GTCTC	5 cut(s)	73, 204, 307, 952, 965
BstMBI	GATC	8 cut(s)	397, 546, 642, 648, 1015, 1405, 1456, 1642
BstMCI	CGRYCG	2 cut(s)	1018, 1459
BstMWI	GCNNNNNNNGC	2 cut(s)	95, 128
BstNI	CCWGG	3 cut(s)	586, 1195, 1664
BstNSI	RCATGY	2 cut(s)	245, 1234
BstPAI	GACNNNNGTC	1 cut(s)	1656
BstSCI	CCNGG	4 cut(s)	508, 584, 1193, 1662
BstSLI	GKGCMC	2 cut(s)	81, 939
BstSNI	TACGTA	1 cut(s)	47
BstUI	CGCG	1 cut(s)	450
BstV2I	GAAGAC	1 cut(s)	162
BsuRI	GGCC	7 cut(s)	494, 590, 750, 896, 1140, 1193, 1245
BtgI	CCRYGG	2 cut(s)	732, 745
BtsCI	GGATG	3 cut(s)	742, 915, 1026
BtsI	GCAGTG	1 cut(s)	579
BtsIMutI	CAGTG	2 cut(s)	26, 579
Cac8I	GCNNGC	1 cut(s)	1400
CciI	TCATGA	2 cut(s)	645, 670
CfoI	GCGC	2 cut(s)	1066, 1398
Cfr13I	GGNCC	2 cut(s)	492, 588
CseI	GACGC	1 cut(s)	1224
Csp6I	GTAC	3 cut(s)	31, 150, 658
CviQI	GTAC	3 cut(s)	31, 150, 658
DdeI	CTNAG	4 cut(s)	208, 291, 378, 792
DinI	GGCGCC	1 cut(s)	1397
DpnI	GATC	8 cut(s)	399, 548, 644, 650, 1017, 1407, 1458, 1644
DpnII	GATC	8 cut(s)	397, 546, 642, 648, 1015, 1405, 1456, 1642
DraIII	CACNNNGTG	2 cut(s)	526, 724
Eam1104I	CTCTTC	4 cut(s)	380, 809, 1016, 1569
EarI	CTCTTC	4 cut(s)	380, 809, 1016, 1569
Ecl136II	GAGCTC	1 cut(s)	1315
Eco105I	TACGTA	1 cut(s)	47
Eco130I	CCWWGG	5 cut(s)	164, 732, 745, 1240, 1565
Eco147I	AGGCCT	2 cut(s)	1140, 1245
Eco24I	GRGCYC	2 cut(s)	377, 1317
Eco53kI	GAGCTC	1 cut(s)	1315
Eco57I	CTGAAG	2 cut(s)	122, 1568
Eco88I	CYCGRG	2 cut(s)	321, 1508
EcoICRI	GAGCTC	1 cut(s)	1315
EcoRII	CCWGG	3 cut(s)	584, 1193, 1662
EcoT14I	CCWWGG	5 cut(s)	164, 732, 745, 1240, 1565
EcoT38I	GRGCYC	2 cut(s)	377, 1317
EgeI	GGCGCC	1 cut(s)	1397
EheI	GGCGCC	1 cut(s)	1397
ErhI	CCWWGG	5 cut(s)	164, 732, 745, 1240, 1565
FalI	AAGNNNNNCTT	8 cut(s)	1077, 1109, 1233, 1265, 1566, 1598, 1701, 1733
FaqI	GGGAC	1 cut(s)	870
FauI	CCCGC	2 cut(s)	522, 1746
FbaI	TGATCA	4 cut(s)	642, 648, 1405, 1642
FblI	GTMKAC	1 cut(s)	342
FokI	GGATG	3 cut(s)	749, 922, 1033
FriOI	GRGCYC	2 cut(s)	377, 1317
FspBI	CTAG	5 cut(s)	183, 557, 1212, 1677, 1734
GlaI	GCGC	2 cut(s)	1065, 1397
GsaI	CCCAGC	1 cut(s)	374
GsuI	CTGGAG	1 cut(s)	1675
HaeII	RGCGCY	1 cut(s)	1399
HaeIII	GGCC	7 cut(s)	494, 590, 750, 896, 1140, 1193, 1245
HapII	CCGG	3 cut(s)	495, 509, 1599
HgaI	GACGC	1 cut(s)	1224
HhaI	GCGC	2 cut(s)	1066, 1398
Hin1I	GRCGYC	1 cut(s)	1396
Hin6I	GCGC	2 cut(s)	1064, 1396
HinP1I	GCGC	2 cut(s)	1064, 1396
HincII	GTYRAC	1 cut(s)	343
HindII	GTYRAC	1 cut(s)	343
HpaII	CCGG	3 cut(s)	495, 509, 1599
HphI	GGTGA	3 cut(s)	469, 1310, 1652
Hpy166II	GTNNAC	4 cut(s)	343, 356, 937, 1057
Hpy188I	TCNGA	6 cut(s)	307, 379, 424, 1020, 1098, 1263
Hpy188III	TCNNGA	8 cut(s)	321, 323, 640, 646, 652, 671, 1454, 1703
Hpy8I	GTNNAC	4 cut(s)	343, 356, 937, 1057
Hpy99I	CGWCG	2 cut(s)	269, 272
HpyAV	CCTTC	6 cut(s)	490, 761, 832, 1018, 1256, 1727
HpyCH4III	ACNGT	2 cut(s)	30, 1551
HpyCH4IV	ACGT	1 cut(s)	46
HpyCH4V	TGCA	6 cut(s)	810, 870, 937, 1129, 1188, 1237
HpyF10VI	GCNNNNNNNGC	2 cut(s)	95, 128
HpyF3I	CTNAG	4 cut(s)	208, 291, 378, 792
HpySE526I	ACGT	1 cut(s)	46
Hsp92I	GRCGYC	1 cut(s)	1396
HspAI	GCGC	2 cut(s)	1064, 1396
KasI	GGCGCC	1 cut(s)	1395
Ksp22I	TGATCA	4 cut(s)	642, 648, 1405, 1642
Kzo9I	GATC	8 cut(s)	397, 546, 642, 648, 1015, 1405, 1456, 1642
LguI	GCTCTTC	2 cut(s)	809, 1016
LmnI	GCTCC	2 cut(s)	360, 1771
LweI	GCATC	2 cut(s)	931, 1569
MaeI	CTAG	5 cut(s)	183, 557, 1212, 1677, 1734
MaeII	ACGT	1 cut(s)	46
MaeIII	GTNAC	5 cut(s)	512, 622, 1156, 1658, 1681
MalI	GATC	8 cut(s)	399, 548, 644, 650, 1017, 1407, 1458, 1644
MbiI	CCGCTC	1 cut(s)	1753
MboI	GATC	8 cut(s)	397, 546, 642, 648, 1015, 1405, 1456, 1642
MfeI	CAATTG	1 cut(s)	1646
MhlI	GDGCHC	6 cut(s)	81, 377, 798, 939, 1005, 1317
MluCI	AATT	8 cut(s)	37, 107, 903, 1111, 1200, 1257, 1382, 1646
MluI	ACGCGT	1 cut(s)	448
Mly113I	GGCGCC	1 cut(s)	1396
MlyI	GAGTC	4 cut(s)	309, 374, 1455, 1666
MmeI	TCCRAC	1 cut(s)	161
MroXI	GAANNNNTTC	1 cut(s)	107
MseI	TTAA	6 cut(s)	350, 831, 846, 993, 1164, 1289
MslI	CAYNNNNRTG	2 cut(s)	63, 246
MspA1I	CMGCKG	3 cut(s)	212, 437, 1726
MspCI	CTTAAG	1 cut(s)	349
MspI	CCGG	3 cut(s)	495, 509, 1599
MspR9I	CCNGG	4 cut(s)	510, 586, 1195, 1664
MunI	CAATTG	1 cut(s)	1646
Mva1269I	GAATGC	1 cut(s)	473
MvaI	CCWGG	3 cut(s)	586, 1195, 1664
MvnI	CGCG	1 cut(s)	450
MwoI	GCNNNNNNNGC	2 cut(s)	95, 128
NarI	GGCGCC	1 cut(s)	1396
NciI	CCSGG	1 cut(s)	510
NcoI	CCATGG	2 cut(s)	732, 745
NdeII	GATC	8 cut(s)	397, 546, 642, 648, 1015, 1405, 1456, 1642
NlaIV	GGNNCC	2 cut(s)	1397, 1773
NmeAIII	GCCGAG	1 cut(s)	922
NmuCI	GTSAC	3 cut(s)	622, 1156, 1658
NspI	RCATGY	2 cut(s)	245, 1234
NspV	TTCGAA	1 cut(s)	1378
PaeR7I	CTCGAG	2 cut(s)	321, 1508
PagI	TCATGA	2 cut(s)	645, 670
PceI	AGGCCT	2 cut(s)	1140, 1245
PciI	ACATGT	2 cut(s)	241, 1230
PciSI	GCTCTTC	2 cut(s)	809, 1016
PctI	GAATGC	1 cut(s)	473
PdmI	GAANNNNTTC	1 cut(s)	107
PfeI	GAWTC	6 cut(s)	18, 221, 236, 308, 467, 1375
Ple19I	CGATCG	2 cut(s)	1018, 1459
PleI	GAGTC	4 cut(s)	308, 373, 1454, 1665
PluTI	GGCGCC	1 cut(s)	1399
PpsI	GAGTC	4 cut(s)	308, 373, 1454, 1665
Ppu21I	YACGTR	1 cut(s)	47
PscI	ACATGT	2 cut(s)	241, 1230
PshAI	GACNNNNGTC	1 cut(s)	1656
PsiI	TTATAA	1 cut(s)	708
Psp124BI	GAGCTC	1 cut(s)	1317
Psp6I	CCWGG	3 cut(s)	584, 1193, 1662
PspFI	CCCAGC	1 cut(s)	370
PspGI	CCWGG	3 cut(s)	584, 1193, 1662
PspN4I	GGNNCC	2 cut(s)	1397, 1773
PspPI	GGNCC	2 cut(s)	492, 588
PspXI	VCTCGAGB	1 cut(s)	1508
PvuI	CGATCG	2 cut(s)	1018, 1459
PvuII	CAGCTG	2 cut(s)	212, 1726
RsaI	GTAC	3 cut(s)	32, 151, 659
RsaNI	GTAC	3 cut(s)	31, 150, 658
RseI	CAYNNNNRTG	2 cut(s)	63, 246
SacI	GAGCTC	1 cut(s)	1317
SalI	GTCGAC	1 cut(s)	341
SapI	GCTCTTC	2 cut(s)	809, 1016
SaqAI	TTAA	6 cut(s)	350, 831, 846, 993, 1164, 1289
Sau3AI	GATC	8 cut(s)	397, 546, 642, 648, 1015, 1405, 1456, 1642
Sau96I	GGNCC	2 cut(s)	492, 588
SchI	GAGTC	4 cut(s)	309, 374, 1455, 1666
ScrFI	CCNGG	4 cut(s)	510, 586, 1195, 1664
SduI	GDGCHC	6 cut(s)	81, 377, 798, 939, 1005, 1317
SfaNI	GCATC	2 cut(s)	931, 1569
SfoI	GGCGCC	1 cut(s)	1397
Sfr274I	CTCGAG	2 cut(s)	321, 1508
SfuI	TTCGAA	1 cut(s)	1378
SlaI	CTCGAG	2 cut(s)	321, 1508
SmiMI	CAYNNNNRTG	2 cut(s)	63, 246
SmlI	CTYRAG	4 cut(s)	321, 349, 1004, 1508
SmoI	CTYRAG	4 cut(s)	321, 349, 1004, 1508
SnaBI	TACGTA	1 cut(s)	47
Sse9I	AATT	8 cut(s)	37, 107, 903, 1111, 1200, 1257, 1382, 1646
SseBI	AGGCCT	2 cut(s)	1140, 1245
SsiI	CCGC	5 cut(s)	405, 437, 529, 769, 1753
SspDI	GGCGCC	1 cut(s)	1395
SspI	AATATT	1 cut(s)	1526
SspMI	CTAG	5 cut(s)	183, 557, 1212, 1677, 1734
SstI	GAGCTC	1 cut(s)	1317
StuI	AGGCCT	2 cut(s)	1140, 1245
StyD4I	CCNGG	4 cut(s)	508, 584, 1193, 1662
StyI	CCWWGG	5 cut(s)	164, 732, 745, 1240, 1565
TaaI	ACNGT	2 cut(s)	30, 1551
TaiI	ACGT	1 cut(s)	49
TaqI	TCGA	9 cut(s)	35, 179, 322, 342, 396, 1014, 1378, 1390, 1509
TasI	AATT	8 cut(s)	37, 107, 903, 1111, 1200, 1257, 1382, 1646
TatI	WGTACW	2 cut(s)	30, 149
TauI	GCSGC	2 cut(s)	408, 771
TfiI	GAWTC	6 cut(s)	18, 221, 236, 308, 467, 1375
Tru1I	TTAA	6 cut(s)	350, 831, 846, 993, 1164, 1289
Tru9I	TTAA	6 cut(s)	350, 831, 846, 993, 1164, 1289
TscAI	CASTG	2 cut(s)	33, 586
TseFI	GTSAC	3 cut(s)	622, 1156, 1658
Tsp45I	GTSAC	3 cut(s)	622, 1156, 1658
TspDTI	ATGAA	7 cut(s)	398, 620, 659, 1037, 1046, 1553, 1685
TspGWI	ACGGA	2 cut(s)	373, 1271
TspRI	CASTG	2 cut(s)	33, 586
Vha464I	CTTAAG	1 cut(s)	349
VneI	GTGCAC	1 cut(s)	935
XapI	RAATTY	3 cut(s)	37, 903, 1382
XceI	RCATGY	2 cut(s)	245, 1234
XhoI	CTCGAG	2 cut(s)	321, 1508
XmiI	GTMKAC	1 cut(s)	342
XmnI	GAANNNNTTC	1 cut(s)	107
XspI	CTAG	5 cut(s)	183, 557, 1212, 1677, 1734

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.