Rroxscaffold_6G00387950

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0009369
Physical Location & Seq
Forward (+)
315946 .. 320271
4326 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00387950.1

Sequence Viewer

Length: 1737 bp
ATGGGATCACCGAAGCCCTCCGCCTTTGTGGCGCCGGTTACTTGTCTCGTGACGGTAATTCGAAGCCCGCATCCTATCGCTACACATAGTGGCGTAAGGGTATATTACGGGAGTAATGGGAGTACTTTAGCTCGGAAGGCTCACTCGCCCTTCGGTTTCAAATGCCCGGTATACGTTGTTGCTGCTCGGCGTTCGGGAGTGAGCAATAGTGACCGCATCCGCTTCTGTCATCACATTTCGGTGGCTCCGGAATCTTGTCATGCGGACATCGGGGAGATATCTACATGGCTGCACACGGATTGGGTTGGATCGGTTTTGAATCTAAACCGTTTCTATACTAAAATTGAGATATCCTCAGCCTCAGCCTCACCGGAGCTGGTGGTGGAGATTCTTTCGCGTTTTCGCAACGATTGGGAATCAGCCTTCACCTTCTTCCTCTGGGCAGGAAAGCAGCGACCCCATTCCGTGCGTGAATACCACTCGATGATCTCCATTCTGGGGAAGATGAGGAAATTCGACACTGCCTGGGCCCTGATCGATGAAATGCGGAGCGGTTCGGGTTTGGGTTCTTCCCTTGTCACTCCTCAAACTCTGTTACTCATGATTCGAAAATACTCTTGTGCTCATGATGTTGGGAGGGCCATTCACACTTTCTATGCCTATAAGCGGTTTGCGTTTGAGGCCGGCATGGATGACTTCCACGGCCTTCTCTCCGCCCTCTGCCGCTACAAAAATGTCCCTGAAGCTGAGCACTTGCTTTTCTGCAACTCACATGTCTTCCCATTTAACACCAAGAGCTTCAACATTATTCTCAACGGCTGGTCTAATATCATCGTTAGTCCCCGCCACGCTGACCGAATTTGGACTGAGATGACCAACCGGGGCGTCCCCCACGATGCCATTTCCTATTCATGTCTCATTTCTTGCCATTCCAAATCCTCCAATCTCACCAAGGTGCTCAACATCTTCCGCCGCATGAACAAAATGAATGTTGAGCCTGACACTAAGGTTTACAATGCCCTCATTTTTGCTCTTGGAAAAGCCGGTCTCTTCAAACAAGCTACCAATCTCATGAAAACCATGCAAGAAAAGGGTCTTGCGCCCAACATTGTCACTTATAACTCCCTCATCAAGACTCTCTGCAAGGCCAGGGAGATTGATCAAGCTACACACATCCTCCATGACATGTTGCACCATGGCCTTGTTCCAACAATTCGGACTTACCATGCCTTCTTCCGTATTTTATCAACCGGGGACCAAGTGTTTGAGCTCTTGGAAAAGATGAAAAACACCGGTTGCCCTCCAAATACTGATACATACATAATGTTGATTCGGAAATTCTGTCGATGGCGCCAGCTTGATAATGTCTTCAAGATTTGGAGTCAGATGAGCGAGAATGGAGTCGGTCCTGATCGCAGCTCATATATTGTGCTCATACATGGGCTCTTTCTAAATGCCAAGTTGGAGGAAGCCCACAAATATTATATGGAGATGAAAGATAGACACTATTTGCCAGAACCAAGGACAGAAGAGATGCTTCAGGCATGGTTGTCTGGTAAGCAGACTTCCAAATTCCAAACAACAGATGGAGAAGTTGATCAAGTGGATTGTACTCAGCCAGCTACGAATACTAAACTAGTAATCTCTAAGAAATCTCCTCAGGAAAAAGATTTCCGTGGGCAACCTGAAAGTAGGAAAGTTGTAAGAGAGCGGGGTTTTTCGTTTTGGGAGCAGTAG

Protein Analysis

578

Amino Acids

65.89

Weight (kDa)

9.32

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 286 - 347 4.8e-07 Pentatricopeptide repeat domain
PPR_long PF17177 287 - 414 5.9e-12 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 299 - 344 1.9e-07 PPR repeat family
PPR_2 PF13041 333 - 382 3.7e-14 PPR repeat family
PPR_3 PF13812 357 - 411 1.4e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 364 - 396 1.4e-09 PPR repeat
PPR_2 PF13041 368 - 413 4.5e-11 PPR repeat family
PPR_2 PF13041 419 - 449 1.1e-06 PPR repeat family
PPR_3 PF13812 424 - 479 9.8e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 443 - 482 3.3e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1119
AccB1I GGYRCC 2 cut(s) 31, 1350
AccBSI CCGCTC 2 cut(s) 552, 1711
AccI GTMKAC 1 cut(s) 171
AccII CGCG 1 cut(s) 397
AccIII TCCGGA 1 cut(s) 247
AclWI GGATC 2 cut(s) 13, 316
AcsI RAATTY 4 cut(s) 512, 858, 1337, 1571
AcuI CTGAAG 2 cut(s) 762, 1523
AcyI GRCGYC 3 cut(s) 32, 885, 1351
AdeI CACNNNGTG 1 cut(s) 89
AfaI GTAC 2 cut(s) 124, 1612
AfiI CCNNNNNNNGG 2 cut(s) 498, 666
AflIII ACRYGT 2 cut(s) 772, 1185
AgeI ACCGGT 1 cut(s) 1292
AgsI TTSAA 5 cut(s) 160, 319, 802, 1054, 1372
AhlI ACTAGT 1 cut(s) 1636
AjnI CCWGG 2 cut(s) 524, 1148
AleI CACNNNNGTG 1 cut(s) 953
Alw21I GWGCWC 5 cut(s) 625, 753, 960, 1272, 1434
Alw26I GTCTC 3 cut(s) 50, 920, 1052
AlwI GGATC 2 cut(s) 13, 316
Aor13HI TCCGGA 1 cut(s) 247
AoxI GGCC 6 cut(s) 528, 639, 681, 703, 1146, 1198
ApaI GGGCCC 1 cut(s) 532
ApeKI GCWGC 4 cut(s) 182, 289, 451, 1416
ApoI RAATTY 4 cut(s) 512, 858, 1337, 1571
ArsI GACNNNNNNTTYG 2 cut(s) 1247, 1279
AsiGI ACCGGT 1 cut(s) 1292
AspLEI GCGC 3 cut(s) 34, 1102, 1353
AspS9I GGNCC 5 cut(s) 528, 529, 639, 1255, 1406
AsuC2I CCSGG 3 cut(s) 167, 881, 1252
AsuHPI GGTGA 3 cut(s) 360, 418, 940
AsuII TTCGAA 2 cut(s) 61, 607
AvaII GGWCC 2 cut(s) 1255, 1406
AxyI CCTNAGG 1 cut(s) 1659
BaeGI GKGCMC 1 cut(s) 532
BanI GGYRCC 2 cut(s) 31, 1350
BanII GRGCYC 3 cut(s) 532, 1272, 1446
BarI GAAGNNNNNNTAC 2 cut(s) 1549, 1581
BauI CACGAG 1 cut(s) 47
BbsI GAAGAC 2 cut(s) 769, 1360
Bbv12I GWGCWC 5 cut(s) 625, 753, 960, 1272, 1434
BbvCI CCTCAGC 2 cut(s) 355, 361
BbvI GCAGC 4 cut(s) 169, 276, 463, 1428
BccI CCATC 2 cut(s) 1341, 1580
BceAI ACGGC 2 cut(s) 718, 832
BciT130I CCWGG 2 cut(s) 526, 1150
BclI TGATCA 2 cut(s) 1159, 1597
BcnI CCSGG 3 cut(s) 167, 881, 1252
BcoDI GTCTC 3 cut(s) 50, 920, 1052
BcuI ACTAGT 1 cut(s) 1636
BfaI CTAG 1 cut(s) 1637
BfoI RGCGCY 2 cut(s) 35, 1354
BglI GCCNNNNNGGC 1 cut(s) 29
BisI GCNGC 6 cut(s) 183, 290, 452, 724, 973, 1417
BlpI GCTNAGC 1 cut(s) 747
BlsI GCNGC 6 cut(s) 184, 291, 453, 725, 974, 1418
BmcAI AGTACT 1 cut(s) 124
Bme1390I CCNGG 5 cut(s) 167, 526, 881, 1150, 1252
Bme18I GGWCC 2 cut(s) 1255, 1406
BmgT120I GGNCC 5 cut(s) 528, 529, 639, 1255, 1406
BmiI GGNNCC 5 cut(s) 33, 246, 530, 1256, 1352
BmrFI CCNGG 5 cut(s) 167, 526, 881, 1150, 1252
BmsI GCATC 4 cut(s) 79, 225, 886, 1524
BpiI GAAGAC 2 cut(s) 769, 1360
BplI GAGNNNNNCTC 2 cut(s) 338, 370
Bpu10I CCTNAGC 2 cut(s) 355, 361
Bpu1102I GCTNAGC 1 cut(s) 747
Bpu14I TTCGAA 2 cut(s) 61, 607
BpuMI CCSGG 3 cut(s) 167, 881, 1252
Bsa29I ATCGAT 1 cut(s) 537
BsaHI GRCGYC 3 cut(s) 32, 885, 1351
BsaI GGTCTC 1 cut(s) 1052
BsaJI CCNNGG 9 cut(s) 525, 700, 880, 951, 1149, 1195, 1251, 1520, 1675
BsaWI WCCGGW 3 cut(s) 247, 370, 1292
BsaXI ACNNNNNCTCC 2 cut(s) 1161, 1191
Bsc4I CCNNNNNNNGG 2 cut(s) 498, 666
Bse118I RCCGGY 4 cut(s) 34, 683, 1043, 1292
Bse21I CCTNAGG 1 cut(s) 1659
BseAI TCCGGA 1 cut(s) 247
BseBI CCWGG 2 cut(s) 526, 1150
BseCI ATCGAT 1 cut(s) 537
BseDI CCNNGG 9 cut(s) 525, 700, 880, 951, 1149, 1195, 1251, 1520, 1675
BseGI GGATG 4 cut(s) 70, 216, 697, 1173
BseLI CCNNNNNNNGG 2 cut(s) 498, 666
BseMII CTCAG 6 cut(s) 369, 375, 738, 858, 1628, 1673
BseRI GAGGAG 2 cut(s) 573, 1647
BseSI GKGCMC 1 cut(s) 532
BseXI GCAGC 4 cut(s) 169, 276, 463, 1428
BsgI GTGCAG 1 cut(s) 275
Bsh1236I CGCG 1 cut(s) 397
BshFI GGCC 6 cut(s) 530, 641, 683, 705, 1148, 1200
BshNI GGYRCC 2 cut(s) 31, 1350
BshTI ACCGGT 1 cut(s) 1292
BshVI ATCGAT 1 cut(s) 537
BsiHKAI GWGCWC 5 cut(s) 625, 753, 960, 1272, 1434
BsiSI CCGG 9 cut(s) 35, 167, 248, 371, 684, 880, 1044, 1251, 1293
BslFI GGGAC 4 cut(s) 722, 825, 872, 1268
BslI CCNNNNNNNGG 2 cut(s) 498, 666
BsmAI GTCTC 3 cut(s) 50, 920, 1052
BsmFI GGGAC 4 cut(s) 722, 825, 872, 1268
BsnI GGCC 6 cut(s) 530, 641, 683, 705, 1148, 1200
Bso31I GGTCTC 1 cut(s) 1052
Bsp119I TTCGAA 2 cut(s) 61, 607
Bsp120I GGGCCC 1 cut(s) 528
Bsp1286I GDGCHC 7 cut(s) 532, 625, 753, 960, 1272, 1434, 1446
Bsp13I TCCGGA 1 cut(s) 247
Bsp143I GATC 7 cut(s) 5, 308, 486, 534, 1159, 1411, 1597
Bsp1720I GCTNAGC 1 cut(s) 747
Bsp19I CCATGG 1 cut(s) 1195
BspANI GGCC 6 cut(s) 530, 641, 683, 705, 1148, 1200
BspCNI CTCAG 6 cut(s) 368, 374, 739, 859, 1627, 1672
BspDI ATCGAT 1 cut(s) 537
BspEI TCCGGA 1 cut(s) 247
BspFNI CGCG 1 cut(s) 397
BspHI TCATGA 3 cut(s) 600, 625, 1071
BspLI GGNNCC 5 cut(s) 33, 246, 530, 1256, 1352
BspPI GGATC 2 cut(s) 13, 316
BspT104I TTCGAA 2 cut(s) 61, 607
BspT107I GGYRCC 2 cut(s) 31, 1350
BspTNI GGTCTC 1 cut(s) 1052
BsrBI CCGCTC 2 cut(s) 552, 1711
BsrFI RCCGGY 4 cut(s) 34, 683, 1043, 1292
BssAI RCCGGY 4 cut(s) 34, 683, 1043, 1292
BssECI CCNNGG 9 cut(s) 525, 700, 880, 951, 1149, 1195, 1251, 1520, 1675
BssMI GATC 7 cut(s) 5, 308, 486, 534, 1159, 1411, 1597
BssNAI GTATAC 1 cut(s) 172
BssNI GRCGYC 3 cut(s) 32, 885, 1351
BssSI CACGAG 1 cut(s) 47
BssT1I CCWWGG 3 cut(s) 951, 1195, 1520
Bst1107I GTATAC 1 cut(s) 172
Bst2BI CACGAG 1 cut(s) 47
Bst2UI CCWGG 2 cut(s) 526, 1150
Bst4CI ACNGT 2 cut(s) 55, 329
Bst6I CTCTTC 2 cut(s) 1055, 1524
BstACI GRCGYC 3 cut(s) 32, 885, 1351
BstBI TTCGAA 2 cut(s) 61, 607
BstC8I GCNNGC 4 cut(s) 68, 685, 1355, 1620
BstDEI CTNAG 8 cut(s) 355, 361, 747, 867, 1005, 1614, 1647, 1659
BstDSI CCRYGG 3 cut(s) 700, 1195, 1675
BstF5I GGATG 4 cut(s) 70, 216, 697, 1173
BstFNI CGCG 1 cut(s) 397
BstH2I RGCGCY 2 cut(s) 35, 1354
BstHHI GCGC 3 cut(s) 34, 1102, 1353
BstKTI GATC 7 cut(s) 8, 311, 489, 537, 1162, 1414, 1600
BstMAI GTCTC 3 cut(s) 50, 920, 1052
BstMBI GATC 7 cut(s) 5, 308, 486, 534, 1159, 1411, 1597
BstMWI GCNNNNNNNGC 3 cut(s) 29, 137, 680
BstNI CCWGG 2 cut(s) 526, 1150
BstNSI RCATGY 2 cut(s) 776, 1189
BstSCI CCNGG 5 cut(s) 165, 524, 879, 1148, 1250
BstSLI GKGCMC 1 cut(s) 532
BstUI CGCG 1 cut(s) 397
BstV1I GCAGC 4 cut(s) 169, 276, 463, 1428
BstV2I GAAGAC 2 cut(s) 769, 1360
BstZ17I GTATAC 1 cut(s) 172
Bsu15I ATCGAT 1 cut(s) 537
Bsu36I CCTNAGG 1 cut(s) 1659
BsuRI GGCC 6 cut(s) 530, 641, 683, 705, 1148, 1200
BsuTUI ATCGAT 1 cut(s) 537
BtgI CCRYGG 3 cut(s) 700, 1195, 1675
BtsCI GGATG 4 cut(s) 70, 216, 697, 1173
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 1 cut(s) 519
Cac8I GCNNGC 4 cut(s) 68, 685, 1355, 1620
CciI TCATGA 3 cut(s) 600, 625, 1071
CfoI GCGC 3 cut(s) 34, 1102, 1353
Cfr10I RCCGGY 4 cut(s) 34, 683, 1043, 1292
Cfr13I GGNCC 5 cut(s) 528, 529, 639, 1255, 1406
ClaI ATCGAT 1 cut(s) 537
CseI GACGC 1 cut(s) 874
Csp6I GTAC 2 cut(s) 123, 1611
CspAI ACCGGT 1 cut(s) 1292
CviQI GTAC 2 cut(s) 123, 1611
DdeI CTNAG 8 cut(s) 355, 361, 747, 867, 1005, 1614, 1647, 1659
DinI GGCGCC 2 cut(s) 33, 1352
DpnI GATC 7 cut(s) 7, 310, 488, 536, 1161, 1413, 1599
DpnII GATC 7 cut(s) 5, 308, 486, 534, 1159, 1411, 1597
DraIII CACNNNGTG 1 cut(s) 89
Eam1104I CTCTTC 2 cut(s) 1055, 1524
EarI CTCTTC 2 cut(s) 1055, 1524
EciI GGCGGA 3 cut(s) 10, 703, 959
Ecl136II GAGCTC 1 cut(s) 1270
Eco130I CCWWGG 3 cut(s) 951, 1195, 1520
Eco24I GRGCYC 3 cut(s) 532, 1272, 1446
Eco31I GGTCTC 1 cut(s) 1052
Eco32I GATATC 2 cut(s) 279, 351
Eco47I GGWCC 2 cut(s) 1255, 1406
Eco53kI GAGCTC 1 cut(s) 1270
Eco57I CTGAAG 2 cut(s) 762, 1523
Eco81I CCTNAGG 1 cut(s) 1659
EcoICRI GAGCTC 1 cut(s) 1270
EcoO109I RGGNCCY 1 cut(s) 529
EcoRII CCWGG 2 cut(s) 524, 1148
EcoRV GATATC 2 cut(s) 279, 351
EcoT14I CCWWGG 3 cut(s) 951, 1195, 1520
EcoT38I GRGCYC 3 cut(s) 532, 1272, 1446
EgeI GGCGCC 2 cut(s) 33, 1352
EheI GGCGCC 2 cut(s) 33, 1352
ErhI CCWWGG 3 cut(s) 951, 1195, 1520
FalI AAGNNNNNCTT 2 cut(s) 1521, 1553
FaqI GGGAC 4 cut(s) 722, 825, 872, 1268
FauI CCCGC 3 cut(s) 75, 851, 1704
FbaI TGATCA 2 cut(s) 1159, 1597
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 6 cut(s) 183, 290, 452, 724, 973, 1417
FokI GGATG 4 cut(s) 57, 203, 704, 1160
FriOI GRGCYC 3 cut(s) 532, 1272, 1446
Fsp4HI GCNGC 6 cut(s) 183, 290, 452, 724, 973, 1417
FspBI CTAG 1 cut(s) 1637
GlaI GCGC 3 cut(s) 33, 1101, 1352
GluI GCNGC 6 cut(s) 183, 290, 452, 724, 973, 1417
HaeII RGCGCY 2 cut(s) 35, 1354
HaeIII GGCC 6 cut(s) 530, 641, 683, 705, 1148, 1200
HapII CCGG 9 cut(s) 35, 167, 248, 371, 684, 880, 1044, 1251, 1293
HgaI GACGC 1 cut(s) 874
HhaI GCGC 3 cut(s) 34, 1102, 1353
Hin1I GRCGYC 3 cut(s) 32, 885, 1351
Hin6I GCGC 3 cut(s) 32, 1100, 1351
HinP1I GCGC 3 cut(s) 32, 1100, 1351
HinfI GANTC 9 cut(s) 251, 319, 388, 416, 604, 1135, 1330, 1381, 1401
HpaII CCGG 9 cut(s) 35, 167, 248, 371, 684, 880, 1044, 1251, 1293
HphI GGTGA 3 cut(s) 360, 418, 940
Hpy166II GTNNAC 2 cut(s) 172, 1012
Hpy188I TCNGA 4 cut(s) 135, 1218, 1335, 1386
Hpy8I GTNNAC 2 cut(s) 172, 1012
HpyAV CCTTC 6 cut(s) 130, 160, 433, 439, 716, 1240
HpyCH4III ACNGT 2 cut(s) 55, 329
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 5 cut(s) 292, 765, 1084, 1143, 1192
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 137, 680
HpyF3I CTNAG 8 cut(s) 355, 361, 747, 867, 1005, 1614, 1647, 1659
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 3 cut(s) 32, 885, 1351
HspAI GCGC 3 cut(s) 32, 1100, 1351
KasI GGCGCC 2 cut(s) 31, 1350
Kpn2I TCCGGA 1 cut(s) 247
KroI GCCGGC 1 cut(s) 683
KroNI GCCGGC 1 cut(s) 685
Ksp22I TGATCA 2 cut(s) 1159, 1597
Kzo9I GATC 7 cut(s) 5, 308, 486, 534, 1159, 1411, 1597
LmnI GCTCC 4 cut(s) 250, 373, 549, 1729
Lsp1109I GCAGC 4 cut(s) 169, 276, 463, 1428
LweI GCATC 4 cut(s) 79, 225, 886, 1524
MaeI CTAG 1 cut(s) 1637
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 6 cut(s) 37, 49, 209, 577, 594, 1111
MalI GATC 7 cut(s) 7, 310, 488, 536, 1161, 1413, 1599
MbiI CCGCTC 2 cut(s) 552, 1711
MboI GATC 7 cut(s) 5, 308, 486, 534, 1159, 1411, 1597
MboII GAAGA 9 cut(s) 424, 514, 561, 769, 958, 1042, 1225, 1360, 1541
MhlI GDGCHC 7 cut(s) 532, 625, 753, 960, 1272, 1434, 1446
MluCI AATT 7 cut(s) 57, 342, 512, 858, 1212, 1337, 1571
Mly113I GGCGCC 2 cut(s) 32, 1351
MlyI GAGTC 3 cut(s) 1129, 1390, 1410
MmeI TCCRAC 3 cut(s) 286, 1232, 1443
MroI TCCGGA 1 cut(s) 247
MroNI GCCGGC 1 cut(s) 683
MseI TTAA 1 cut(s) 786
MslI CAYNNNNRTG 2 cut(s) 239, 953
MspI CCGG 9 cut(s) 35, 167, 248, 371, 684, 880, 1044, 1251, 1293
MspR9I CCNGG 5 cut(s) 167, 526, 881, 1150, 1252
MvaI CCWGG 2 cut(s) 526, 1150
MvnI CGCG 1 cut(s) 397
MwoI GCNNNNNNNGC 3 cut(s) 29, 137, 680
NaeI GCCGGC 1 cut(s) 685
NarI GGCGCC 2 cut(s) 32, 1351
NciI CCSGG 3 cut(s) 167, 881, 1252
NcoI CCATGG 1 cut(s) 1195
NdeII GATC 7 cut(s) 5, 308, 486, 534, 1159, 1411, 1597
NgoMIV GCCGGC 1 cut(s) 683
NlaIV GGNNCC 5 cut(s) 33, 246, 530, 1256, 1352
NmeAIII GCCGAG 1 cut(s) 166
NmuCI GTSAC 4 cut(s) 49, 209, 577, 1111
NspI RCATGY 2 cut(s) 776, 1189
NspV TTCGAA 2 cut(s) 61, 607
OliI CACNNNNGTG 1 cut(s) 953
PagI TCATGA 3 cut(s) 600, 625, 1071
PciI ACATGT 2 cut(s) 772, 1185
PdiI GCCGGC 1 cut(s) 685
PfeI GAWTC 6 cut(s) 251, 319, 388, 416, 604, 1330
PinAI ACCGGT 1 cut(s) 1292
PkrI GCNGC 6 cut(s) 184, 291, 453, 725, 974, 1418
PleI GAGTC 3 cut(s) 1129, 1389, 1409
PluTI GGCGCC 2 cut(s) 35, 1354
PpsI GAGTC 3 cut(s) 1129, 1389, 1409
PscI ACATGT 2 cut(s) 772, 1185
PsiI TTATAA 1 cut(s) 1119
Psp124BI GAGCTC 1 cut(s) 1272
Psp6I CCWGG 2 cut(s) 524, 1148
PspGI CCWGG 2 cut(s) 524, 1148
PspN4I GGNNCC 5 cut(s) 33, 246, 530, 1256, 1352
PspOMI GGGCCC 1 cut(s) 528
PspPI GGNCC 5 cut(s) 528, 529, 639, 1255, 1406
RsaI GTAC 2 cut(s) 124, 1612
RsaNI GTAC 2 cut(s) 123, 1611
RseI CAYNNNNRTG 2 cut(s) 239, 953
SacI GAGCTC 1 cut(s) 1272
SaqAI TTAA 1 cut(s) 786
SatI GCNGC 6 cut(s) 183, 290, 452, 724, 973, 1417
Sau3AI GATC 7 cut(s) 5, 308, 486, 534, 1159, 1411, 1597
Sau96I GGNCC 5 cut(s) 528, 529, 639, 1255, 1406
ScaI AGTACT 1 cut(s) 124
SchI GAGTC 3 cut(s) 1129, 1390, 1410
ScrFI CCNGG 5 cut(s) 167, 526, 881, 1150, 1252
SduI GDGCHC 7 cut(s) 532, 625, 753, 960, 1272, 1434, 1446
SfaNI GCATC 4 cut(s) 79, 225, 886, 1524
SfoI GGCGCC 2 cut(s) 33, 1352
SfuI TTCGAA 2 cut(s) 61, 607
SinI GGWCC 2 cut(s) 1255, 1406
SmiMI CAYNNNNRTG 2 cut(s) 239, 953
SpeI ACTAGT 1 cut(s) 1636
Sse9I AATT 7 cut(s) 57, 342, 512, 858, 1212, 1337, 1571
SspDI GGCGCC 2 cut(s) 31, 1350
SspI AATATT 1 cut(s) 1481
SspMI CTAG 1 cut(s) 1637
SstI GAGCTC 1 cut(s) 1272
StyD4I CCNGG 5 cut(s) 165, 524, 879, 1148, 1250
StyI CCWWGG 3 cut(s) 951, 1195, 1520
TaaI ACNGT 2 cut(s) 55, 329
TaiI ACGT 1 cut(s) 177
TaqI TCGA 6 cut(s) 61, 482, 516, 537, 607, 1345
TaqII GACCGA 2 cut(s) 870, 1394
TasI AATT 7 cut(s) 57, 342, 512, 858, 1212, 1337, 1571
TatI WGTACW 2 cut(s) 122, 1610
TauI GCSGC 2 cut(s) 726, 975
TfiI GAWTC 6 cut(s) 251, 319, 388, 416, 604, 1330
Tru1I TTAA 1 cut(s) 786
Tru9I TTAA 1 cut(s) 786
TscAI CASTG 1 cut(s) 526
TseFI GTSAC 4 cut(s) 49, 209, 577, 1111
TseI GCWGC 4 cut(s) 182, 289, 451, 1416
Tsp45I GTSAC 4 cut(s) 49, 209, 577, 1111
TspDTI ATGAA 7 cut(s) 555, 900, 992, 1001, 1088, 1298, 1508
TspGWI ACGGA 4 cut(s) 311, 454, 1226, 1664
TspRI CASTG 1 cut(s) 526
VpaK11BI GGWCC 2 cut(s) 1255, 1406
XapI RAATTY 4 cut(s) 512, 858, 1337, 1571
XceI RCATGY 2 cut(s) 776, 1189
XcmI CCANNNNNNNNNTGG 1 cut(s) 1583
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 1637
ZrmI AGTACT 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.