pycom12g04490

calmodulin-binding family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011034
Physical Location & Seq
Forward (+)
4360179 .. 4362769
2591 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g04490.1

Sequence Viewer

Length: 1845 bp
ATGGGGTTTCCAGTATCCTGCCCACTTGCAGATTTTGATGATTTGGATAGTCATTGTAGAGCTGTTCATGTAAGTCCAGTTAGTCTTACGAAAGAAGAGAGGAAGCCATTGCAGTCATTGAGCTCCAACACCTGCTGTGATTCAGAACCAACGACAACCATGAAATCACCTGGTTCAGTAAAGATGAACTTTGAAGAACCACTTAGCTTGAAAGAGATGGAATTAGACGCCATTTCAGCAAACTCACCTTCCAGGGATATGCAAAACAATGTGTCTGCTAGAGCAGAAAGCGGTATTGGAGAGAAACAGCAGACATCCGAGAACTCATTAAAGGAGCCCAAATACCAGGCTGCATTGAAGCTGCAGAAAGTTTATAAGAGTTTTCGCACCAGAAGACAGCTTGCGGATTGTGCAATTCTTGTGGAGCAGAGATGGTGGAAGGTGCTCGATTTTGCTGAACTAAGGCAGAGCTCTATATCATTTTTCGACATTCAGAAACCTGAGACAGCTGTTTCTCGCTGGTCTCGGGCAAGAACTAAAGCTGCCAAGGTGGGAAAAGGTTTGTCAAAGGATAAAAGGGCGCGTAAACTTGCGTTGCAGCATTGGCTTGAGGCAATTGATCCAAGACATCGCTATGGCCATAATCTTCACTTTTACTACGCACGATGGCTCCATTGCGAAAGTAAACAGCCATTCTTCTATTGGCTTGATATAGGAGATGGAAGAGAATTAAACCATGAAACATGCCCTAGGTCGAAGCTTCAAGCGCAATGTATCAAGTATCTTGGCCCGACCGAAAGGGAGGCTTATGAAGTTGTAGTGCAGGATGGGAAGTTCATGTACAAGCAGATTGGAAACTTTCTTGATACATCAGAACCTAAAGACACAAAGTGGATATTTGTGCTTAGCACATCCAAGACCTTGTATGTTGGACAGAAGAGCAAGGGGACGTTTCAACATTCGAGTTTCTTGGCAGGCGGAGCCACTCTTTCTGCCGGAAGATTAGTAGTGGAACAAGGAATTCTGAAGTCAGTTTGGCCTCACAGTGGGCATTATCTCCCCACGGAAGAAAACTTCCAGGAATTTATAATGTTCCTTATGGAACACAACGTCGATCTCACAAATGTTAATAGAAGTCCAGCGGATGACGAAGAAGGAGGCTGTACAAAGAAGAGCAGCAACATCCGAAACAGCCCACGAAAACCAAAAGAAACCACAACAACAAAAGACGAAGGCACAGAGCAAGATAACACTGACTCAAGAAATGAGGAGTCTAACTCAGCAGAAAATCCCAATCCTATTCTGTCAAGATTACCTGAAAGATTCTGCTCAAAGATAGCCAAACTCGAAATACCACAAAGGAACGATGCCTTTGACATTTTGAAGGACGGAATACCAATAACCTGTCGCGAATACTTTCCAGACTCTGCCTCGGATGATGGTTACGAAACAGCTGAAGAATCAGTCCTGACAGAGGAAGACTTCATGGTCCCTAAACTGAACTTGTTCGAAGAAGATCGATTCGAAGAAGATGAAAAGCCAGTACCAAAGGAAACGATCGATCACATGCAGAGGATTGATTCACACAAGGGAATGAAGTCATACCAGTTGGCGCAGCATTTAACCAGCAGGTGGACAACGGGAGCTGGACCACGAATAGGTTGCATGAGGGATTATCCTTCAGAGCTTCAGTTCCGGGTTTTAGAGCAAGCCAACTTGTCTCCGAGAAGGATAGGAAGAAGCCCCCGACTCTTGATTCCGTCTCCTTTGAGTAAAGAGGAAACCTCGGTGAAAAGTCTCCTTGGCCCCCGCTCGAGGAGCAAGCGACACAACACCATAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

615

Amino Acids

70.02

Weight (kDa)

7.24

Isoelectric Point (pI)

57.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58480
fragaria_vesca FvH4_6g21330 FvH4_6g21330 FvH4_6g21330
malus_domestica MD12G1050400.v1.1 MD14G1048900.v1.1
prunus_persica Prupe.7G086400_v2.0.a1
pyrus_communis pycom12g04490 pycom14g04000
rosa_chinensis RchiOBHm_Chr3g0477941
rosa_laevigata RLG00000023678
rosa_multiflora Rmu_sc0005991.1_g000009 Rmu_sc0028100.1_g000001
rosa_roxburghii Rroxscaffold_6G00403580
rosa_rugosa Rorug03G0164000
rosa_samantha Rh3AG214800 Rh3BG248400 Rh3CG242400 Rh3DG241800
rosa_wichuraiana Rw3G019360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 375, 1088
AarI CACCTGC 2 cut(s) 140, 1620
AasI GACNNNNNNGTC 1 cut(s) 1487
Acc36I ACCTGC 2 cut(s) 140, 1620
AccB7I CCANNNNNTGG 1 cut(s) 1632
AccBSI CCGCTC 1 cut(s) 1812
AccII CGCG 2 cut(s) 583, 1410
AciI CCGC 5 cut(s) 291, 404, 978, 1142, 1810
AclWI GGATC 1 cut(s) 614
AcoI YGGCCR 1 cut(s) 637
AcsI RAATTY 2 cut(s) 1020, 1082
AcuI CTGAAG 4 cut(s) 1046, 1476, 1665, 1673
AcyI GRCGYC 1 cut(s) 228
AdeI CACNNNGTG 1 cut(s) 891
AfaI GTAC 3 cut(s) 842, 1165, 1545
AfiI CCNNNNNNNGG 5 cut(s) 1046, 1474, 1632, 1658, 1815
AgsI TTSAA 6 cut(s) 194, 211, 358, 764, 956, 1384
AjnI CCWGG 4 cut(s) 169, 251, 345, 1077
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
AloI GAACNNNNNNTCC 2 cut(s) 818, 850
Alw21I GWGCWC 3 cut(s) 125, 447, 473
Alw26I GTCTC 5 cut(s) 497, 528, 1725, 1767, 1802
AlwI GGATC 1 cut(s) 614
AlwNI CAGNNNCTG 1 cut(s) 1427
Ama87I CYCGRG 2 cut(s) 525, 1813
AoxI GGCC 4 cut(s) 637, 787, 1037, 1804
ApeKI GCWGC 6 cut(s) 350, 361, 542, 598, 1176, 1615
ApoI RAATTY 2 cut(s) 1020, 1082
Asp700I GAANNNNTTC 2 cut(s) 1416, 1505
AspA2I CCTAGG 1 cut(s) 749
AspLEI GCGC 3 cut(s) 583, 769, 1615
AspS9I GGNCC 4 cut(s) 788, 1489, 1649, 1805
AsuC2I CCSGG 1 cut(s) 1697
AsuHPI GGTGA 3 cut(s) 159, 237, 1801
AsuII TTCGAA 2 cut(s) 1509, 1524
AvaI CYCGRG 2 cut(s) 525, 1813
AvaII GGWCC 2 cut(s) 1489, 1649
AvrII CCTAGG 1 cut(s) 749
BalI TGGCCA 1 cut(s) 639
BanII GRGCYC 3 cut(s) 125, 339, 473
BarI GAAGNNNNNNTAC 2 cut(s) 824, 856
BbsI GAAGAC 2 cut(s) 400, 1485
Bbv12I GWGCWC 3 cut(s) 125, 447, 473
BbvI GCAGC 6 cut(s) 337, 348, 529, 610, 1188, 1627
BccI CCATC 6 cut(s) 211, 426, 660, 713, 821, 1433
BcgI CGANNNNNNTGC 2 cut(s) 1644, 1678
BciT130I CCWGG 4 cut(s) 171, 253, 347, 1079
BciVI GTATCC 1 cut(s) 25
BcnI CCSGG 1 cut(s) 1697
BcoDI GTCTC 5 cut(s) 497, 528, 1725, 1767, 1802
BfaI CTAG 2 cut(s) 279, 750
BfmI CTRYAG 1 cut(s) 362
BfuAI ACCTGC 2 cut(s) 140, 1620
BfuI GTATCC 1 cut(s) 25
BisI GCNGC 6 cut(s) 351, 362, 543, 599, 1177, 1616
BlnI CCTAGG 1 cut(s) 749
BlpI GCTNAGC 1 cut(s) 905
BlsI GCNGC 6 cut(s) 352, 363, 544, 600, 1178, 1617
Bme1390I CCNGG 5 cut(s) 171, 253, 347, 1079, 1697
Bme18I GGWCC 2 cut(s) 1489, 1649
BmeT110I CYCGRG 2 cut(s) 525, 1813
BmgT120I GGNCC 4 cut(s) 788, 1489, 1649, 1805
BmiI GGNNCC 5 cut(s) 336, 671, 982, 1491, 1807
BmrFI CCNGG 5 cut(s) 171, 253, 347, 1079, 1697
BmsI GCATC 1 cut(s) 1357
BpiI GAAGAC 2 cut(s) 400, 1485
BplI GAGNNNNNCTC 4 cut(s) 1262, 1294, 1769, 1801
Bpu1102I GCTNAGC 1 cut(s) 905
Bpu14I TTCGAA 2 cut(s) 1509, 1524
BpuEI CTTGAG 2 cut(s) 629, 1243
BpuMI CCSGG 1 cut(s) 1697
Bsa29I ATCGAT 2 cut(s) 1519, 1560
BsaHI GRCGYC 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 528
BsaJI CCNNGG 7 cut(s) 252, 546, 749, 1062, 1431, 1785, 1801
BsaXI ACNNNNNCTCC 2 cut(s) 654, 684
Bsc4I CCNNNNNNNGG 5 cut(s) 1046, 1474, 1632, 1658, 1815
Bse1I ACTGG 4 cut(s) 11, 77, 1541, 1606
Bse3DI GCAATG 3 cut(s) 107, 673, 776
BseBI CCWGG 4 cut(s) 171, 253, 347, 1079
BseCI ATCGAT 2 cut(s) 1519, 1560
BseDI CCNNGG 7 cut(s) 252, 546, 749, 1062, 1431, 1785, 1801
BseGI GGATG 6 cut(s) 314, 832, 911, 1150, 1182, 1441
BseLI CCNNNNNNNGG 5 cut(s) 1046, 1474, 1632, 1658, 1815
BseMI GCAATG 3 cut(s) 107, 673, 776
BseMII CTCAG 2 cut(s) 492, 1293
BseNI ACTGG 4 cut(s) 11, 77, 1541, 1606
BseRI GAGGAG 2 cut(s) 1283, 1831
BseXI GCAGC 6 cut(s) 337, 348, 529, 610, 1188, 1627
BsgI GTGCAG 1 cut(s) 842
Bsh1236I CGCG 2 cut(s) 583, 1410
Bsh1285I CGRYCG 2 cut(s) 795, 1560
BshFI GGCC 4 cut(s) 639, 789, 1039, 1806
BshVI ATCGAT 2 cut(s) 1519, 1560
BsiEI CGRYCG 2 cut(s) 795, 1560
BsiHKAI GWGCWC 3 cut(s) 125, 447, 473
BsiHKCI CYCGRG 2 cut(s) 525, 1813
BsiSI CCGG 2 cut(s) 996, 1696
BslFI GGGAC 2 cut(s) 961, 1475
BslI CCNNNNNNNGG 5 cut(s) 1046, 1474, 1632, 1658, 1815
BsmAI GTCTC 5 cut(s) 497, 528, 1725, 1767, 1802
BsmBI CGTCTC 1 cut(s) 1767
BsmFI GGGAC 2 cut(s) 961, 1475
BsnI GGCC 4 cut(s) 639, 789, 1039, 1806
Bso31I GGTCTC 1 cut(s) 528
BsoBI CYCGRG 2 cut(s) 525, 1813
Bsp119I TTCGAA 2 cut(s) 1509, 1524
Bsp1286I GDGCHC 4 cut(s) 125, 339, 447, 473
Bsp1407I TGTACA 2 cut(s) 840, 1163
Bsp143I GATC 5 cut(s) 619, 1114, 1516, 1557, 1561
Bsp1720I GCTNAGC 1 cut(s) 905
Bsp68I TCGCGA 1 cut(s) 1410
BspACI CCGC 5 cut(s) 291, 404, 978, 1142, 1810
BspANI GGCC 4 cut(s) 639, 789, 1039, 1806
BspCNI CTCAG 2 cut(s) 493, 1292
BspDI ATCGAT 2 cut(s) 1519, 1560
BspFNI CGCG 2 cut(s) 583, 1410
BspLI GGNNCC 5 cut(s) 336, 671, 982, 1491, 1807
BspMAI CTGCAG 1 cut(s) 366
BspMI ACCTGC 2 cut(s) 140, 1620
BspPI GGATC 1 cut(s) 614
BspQI GCTCTTC 2 cut(s) 932, 1166
BspT104I TTCGAA 2 cut(s) 1509, 1524
BspTNI GGTCTC 1 cut(s) 528
BsrBI CCGCTC 1 cut(s) 1812
BsrDI GCAATG 3 cut(s) 107, 673, 776
BsrGI TGTACA 2 cut(s) 840, 1163
BsrI ACTGG 4 cut(s) 11, 77, 1541, 1606
BssECI CCNNGG 7 cut(s) 252, 546, 749, 1062, 1431, 1785, 1801
BssMI GATC 5 cut(s) 619, 1114, 1516, 1557, 1561
BssNI GRCGYC 1 cut(s) 228
BssT1I CCWWGG 3 cut(s) 546, 749, 1801
Bst2UI CCWGG 4 cut(s) 171, 253, 347, 1079
Bst4CI ACNGT 1 cut(s) 1046
Bst6I CTCTTC 4 cut(s) 90, 718, 932, 1166
BstACI GRCGYC 1 cut(s) 228
BstAUI TGTACA 2 cut(s) 840, 1163
BstBI TTCGAA 2 cut(s) 1509, 1524
BstC8I GCNNGC 4 cut(s) 402, 976, 1710, 1823
BstDEI CTNAG 5 cut(s) 203, 461, 501, 905, 1279
BstDSI CCRYGG 1 cut(s) 1062
BstENI CCTNNNNNAGG 1 cut(s) 1472
BstF5I GGATG 6 cut(s) 314, 832, 911, 1150, 1182, 1441
BstFNI CGCG 2 cut(s) 583, 1410
BstHHI GCGC 3 cut(s) 583, 769, 1615
BstKTI GATC 5 cut(s) 622, 1117, 1519, 1560, 1564
BstMAI GTCTC 5 cut(s) 497, 528, 1725, 1767, 1802
BstMBI GATC 5 cut(s) 619, 1114, 1516, 1557, 1561
BstMCI CGRYCG 2 cut(s) 795, 1560
BstMWI GCNNNNNNNGC 6 cut(s) 236, 410, 604, 766, 980, 1818
BstNI CCWGG 4 cut(s) 171, 253, 347, 1079
BstNSI RCATGY 2 cut(s) 747, 1570
BstSCI CCNGG 5 cut(s) 169, 251, 345, 1077, 1695
BstSFI CTRYAG 1 cut(s) 362
BstUI CGCG 2 cut(s) 583, 1410
BstV1I GCAGC 6 cut(s) 337, 348, 529, 610, 1188, 1627
BstV2I GAAGAC 2 cut(s) 400, 1485
Bsu15I ATCGAT 2 cut(s) 1519, 1560
BsuI GTATCC 1 cut(s) 25
BsuRI GGCC 4 cut(s) 639, 789, 1039, 1806
BsuTUI ATCGAT 2 cut(s) 1519, 1560
BtgI CCRYGG 1 cut(s) 1062
BtgZI GCGATG 1 cut(s) 614
BtsCI GGATG 6 cut(s) 314, 832, 911, 1150, 1182, 1441
BtsIMutI CAGTG 2 cut(s) 1051, 1251
BtuMI TCGCGA 1 cut(s) 1410
BveI ACCTGC 2 cut(s) 140, 1620
Cac8I GCNNGC 4 cut(s) 402, 976, 1710, 1823
CaiI CAGNNNCTG 1 cut(s) 1427
CfoI GCGC 3 cut(s) 583, 769, 1615
Cfr13I GGNCC 4 cut(s) 788, 1489, 1649, 1805
ClaI ATCGAT 2 cut(s) 1519, 1560
CseI GACGC 1 cut(s) 236
CsiI ACCWGGT 1 cut(s) 169
Csp6I GTAC 3 cut(s) 841, 1164, 1544
CspCI CAANNNNNGTGG 4 cut(s) 189, 224, 402, 437
CviAII CATG 8 cut(s) 68, 160, 737, 744, 838, 1486, 1567, 1666
CviQI GTAC 3 cut(s) 841, 1164, 1544
DdeI CTNAG 5 cut(s) 203, 461, 501, 905, 1279
DpnI GATC 5 cut(s) 621, 1116, 1518, 1559, 1563
DpnII GATC 5 cut(s) 619, 1114, 1516, 1557, 1561
DraIII CACNNNGTG 1 cut(s) 891
DrdI GACNNNNNNGTC 1 cut(s) 1487
DseDI GACNNNNNNGTC 1 cut(s) 1487
EaeI YGGCCR 1 cut(s) 637
Eam1104I CTCTTC 4 cut(s) 90, 718, 932, 1166
EarI CTCTTC 4 cut(s) 90, 718, 932, 1166
EciI GGCGGA 1 cut(s) 993
Ecl136II GAGCTC 2 cut(s) 123, 471
Eco130I CCWWGG 3 cut(s) 546, 749, 1801
Eco24I GRGCYC 3 cut(s) 125, 339, 473
Eco31I GGTCTC 1 cut(s) 528
Eco47I GGWCC 2 cut(s) 1489, 1649
Eco53kI GAGCTC 2 cut(s) 123, 471
Eco57I CTGAAG 4 cut(s) 1046, 1476, 1665, 1673
Eco88I CYCGRG 2 cut(s) 525, 1813
EcoICRI GAGCTC 2 cut(s) 123, 471
EcoNI CCTNNNNNAGG 1 cut(s) 1472
EcoRI GAATTC 1 cut(s) 1020
EcoRII CCWGG 4 cut(s) 169, 251, 345, 1077
EcoT14I CCWWGG 3 cut(s) 546, 749, 1801
EcoT38I GRGCYC 3 cut(s) 125, 339, 473
ErhI CCWWGG 3 cut(s) 546, 749, 1801
Esp3I CGTCTC 1 cut(s) 1767
FaeI CATG 8 cut(s) 71, 163, 740, 747, 841, 1489, 1570, 1669
FalI AAGNNNNNCTT 6 cut(s) 173, 205, 186, 218, 790, 822
FaqI GGGAC 2 cut(s) 961, 1475
FatI CATG 8 cut(s) 67, 159, 736, 743, 837, 1485, 1566, 1665
FauI CCCGC 1 cut(s) 1817
Fnu4HI GCNGC 6 cut(s) 351, 362, 543, 599, 1177, 1616
FokI GGATG 6 cut(s) 301, 839, 898, 1157, 1169, 1448
FriOI GRGCYC 3 cut(s) 125, 339, 473
Fsp4HI GCNGC 6 cut(s) 351, 362, 543, 599, 1177, 1616
FspBI CTAG 2 cut(s) 279, 750
GlaI GCGC 3 cut(s) 582, 768, 1614
GluI GCNGC 6 cut(s) 351, 362, 543, 599, 1177, 1616
HaeIII GGCC 4 cut(s) 639, 789, 1039, 1806
HapII CCGG 2 cut(s) 996, 1696
HgaI GACGC 1 cut(s) 236
HhaI GCGC 3 cut(s) 583, 769, 1615
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 8 cut(s) 71, 163, 740, 747, 841, 1489, 1570, 1669
Hin6I GCGC 3 cut(s) 581, 767, 1613
HinP1I GCGC 3 cut(s) 581, 767, 1613
HindIII AAGCTT 1 cut(s) 758
HpaII CCGG 2 cut(s) 996, 1696
HphI GGTGA 3 cut(s) 159, 237, 1801
Hpy166II GTNNAC 3 cut(s) 587, 686, 1635
Hpy188I TCNGA 9 cut(s) 145, 319, 495, 874, 1026, 1187, 1435, 1684, 1725
Hpy188III TCNNGA 7 cut(s) 863, 1260, 1308, 1409, 1421, 1468, 1753
Hpy8I GTNNAC 3 cut(s) 587, 686, 1635
Hpy99I CGWCG 1 cut(s) 1115
HpyAV CCTTC 7 cut(s) 258, 433, 1148, 1226, 1378, 1689, 1722
HpyCH4III ACNGT 1 cut(s) 1046
HpyCH4IV ACGT 2 cut(s) 950, 1110
HpyF10VI GCNNNNNNNGC 6 cut(s) 236, 410, 604, 766, 980, 1818
HpyF3I CTNAG 5 cut(s) 203, 461, 501, 905, 1279
HpySE526I ACGT 2 cut(s) 950, 1110
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 8 cut(s) 71, 163, 740, 747, 841, 1489, 1570, 1669
HspAI GCGC 3 cut(s) 581, 767, 1613
Kzo9I GATC 5 cut(s) 619, 1114, 1516, 1557, 1561
LguI GCTCTTC 2 cut(s) 932, 1166
LmnI GCTCC 7 cut(s) 128, 334, 424, 675, 980, 1643, 1818
Lsp1109I GCAGC 6 cut(s) 337, 348, 529, 610, 1188, 1627
LweI GCATC 1 cut(s) 1357
MabI ACCWGGT 1 cut(s) 169
MaeI CTAG 2 cut(s) 279, 750
MaeII ACGT 2 cut(s) 950, 1110
MaeIII GTNAC 1 cut(s) 1442
MalI GATC 5 cut(s) 621, 1116, 1518, 1559, 1563
MbiI CCGCTC 1 cut(s) 1812
MboI GATC 5 cut(s) 619, 1114, 1516, 1557, 1561
MfeI CAATTG 1 cut(s) 615
MhlI GDGCHC 4 cut(s) 125, 339, 447, 473
MlsI TGGCCA 1 cut(s) 639
MluCI AATT 6 cut(s) 221, 414, 615, 728, 1020, 1082
MluNI TGGCCA 1 cut(s) 639
MlyI GAGTC 4 cut(s) 1250, 1280, 1418, 1743
MmeI TCCRAC 2 cut(s) 150, 910
Mox20I TGGCCA 1 cut(s) 639
MroXI GAANNNNTTC 2 cut(s) 1416, 1505
MscI TGGCCA 1 cut(s) 639
MseI TTAA 4 cut(s) 329, 731, 1128, 1622
MslI CAYNNNNRTG 1 cut(s) 633
Msp20I TGGCCA 1 cut(s) 639
MspA1I CMGCKG 3 cut(s) 509, 1142, 1454
MspI CCGG 2 cut(s) 996, 1696
MspR9I CCNGG 5 cut(s) 171, 253, 347, 1079, 1697
MunI CAATTG 1 cut(s) 615
MvaI CCWGG 4 cut(s) 171, 253, 347, 1079
MvnI CGCG 2 cut(s) 583, 1410
MwoI GCNNNNNNNGC 6 cut(s) 236, 410, 604, 766, 980, 1818
NciI CCSGG 1 cut(s) 1697
NdeII GATC 5 cut(s) 619, 1114, 1516, 1557, 1561
NlaIII CATG 8 cut(s) 71, 163, 740, 747, 841, 1489, 1570, 1669
NlaIV GGNNCC 5 cut(s) 336, 671, 982, 1491, 1807
NruI TCGCGA 1 cut(s) 1410
NspI RCATGY 2 cut(s) 747, 1570
NspV TTCGAA 2 cut(s) 1509, 1524
PaeR7I CTCGAG 1 cut(s) 1813
PaqCI CACCTGC 2 cut(s) 140, 1620
PciSI GCTCTTC 2 cut(s) 932, 1166
PdmI GAANNNNTTC 2 cut(s) 1416, 1505
PfeI GAWTC 6 cut(s) 140, 1323, 1460, 1521, 1580, 1756
PflMI CCANNNNNTGG 1 cut(s) 1632
PfoI TCCNGGA 1 cut(s) 1077
PkrI GCNGC 6 cut(s) 352, 363, 544, 600, 1178, 1617
Ple19I CGATCG 1 cut(s) 1560
PleI GAGTC 4 cut(s) 1250, 1279, 1418, 1743
PpsI GAGTC 4 cut(s) 1250, 1279, 1418, 1743
PsiI TTATAA 2 cut(s) 375, 1088
Psp124BI GAGCTC 2 cut(s) 125, 473
Psp6I CCWGG 4 cut(s) 169, 251, 345, 1077
PspGI CCWGG 4 cut(s) 169, 251, 345, 1077
PspN4I GGNNCC 5 cut(s) 336, 671, 982, 1491, 1807
PspPI GGNCC 4 cut(s) 788, 1489, 1649, 1805
PspXI VCTCGAGB 1 cut(s) 1813
PstI CTGCAG 1 cut(s) 366
PstNI CAGNNNCTG 1 cut(s) 1427
PvuI CGATCG 1 cut(s) 1560
PvuII CAGCTG 2 cut(s) 509, 1454
RruI TCGCGA 1 cut(s) 1410
RsaI GTAC 3 cut(s) 842, 1165, 1545
RsaNI GTAC 3 cut(s) 841, 1164, 1544
RseI CAYNNNNRTG 1 cut(s) 633
SacI GAGCTC 2 cut(s) 125, 473
SapI GCTCTTC 2 cut(s) 932, 1166
SaqAI TTAA 4 cut(s) 329, 731, 1128, 1622
SatI GCNGC 6 cut(s) 351, 362, 543, 599, 1177, 1616
Sau3AI GATC 5 cut(s) 619, 1114, 1516, 1557, 1561
Sau96I GGNCC 4 cut(s) 788, 1489, 1649, 1805
SchI GAGTC 4 cut(s) 1250, 1280, 1418, 1743
ScrFI CCNGG 5 cut(s) 171, 253, 347, 1079, 1697
SduI GDGCHC 4 cut(s) 125, 339, 447, 473
SexAI ACCWGGT 1 cut(s) 169
SfaNI GCATC 1 cut(s) 1357
SfcI CTRYAG 1 cut(s) 362
Sfr274I CTCGAG 1 cut(s) 1813
SfuI TTCGAA 2 cut(s) 1509, 1524
SinI GGWCC 2 cut(s) 1489, 1649
SlaI CTCGAG 1 cut(s) 1813
SmiMI CAYNNNNRTG 1 cut(s) 633
SmlI CTYRAG 3 cut(s) 608, 1258, 1813
SmoI CTYRAG 3 cut(s) 608, 1258, 1813
Sse9I AATT 6 cut(s) 221, 414, 615, 728, 1020, 1082
SsiI CCGC 5 cut(s) 291, 404, 978, 1142, 1810
SspMI CTAG 2 cut(s) 279, 750
SstI GAGCTC 2 cut(s) 125, 473
StyD4I CCNGG 5 cut(s) 169, 251, 345, 1077, 1695
StyI CCWWGG 3 cut(s) 546, 749, 1801
TaaI ACNGT 1 cut(s) 1046
TaiI ACGT 2 cut(s) 953, 1113
TaqII GACCGA 1 cut(s) 809
TasI AATT 6 cut(s) 221, 414, 615, 728, 1020, 1082
TatI WGTACW 2 cut(s) 840, 1163
TfiI GAWTC 6 cut(s) 140, 1323, 1460, 1521, 1580, 1756
Tru1I TTAA 4 cut(s) 329, 731, 1128, 1622
Tru9I TTAA 4 cut(s) 329, 731, 1128, 1622
TscAI CASTG 2 cut(s) 1051, 1258
TseI GCWGC 6 cut(s) 350, 361, 542, 598, 1176, 1615
TspDTI ATGAA 9 cut(s) 56, 176, 200, 753, 825, 826, 1474, 1548, 1610
TspGWI ACGGA 3 cut(s) 1079, 1404, 1749
TspRI CASTG 2 cut(s) 1051, 1258
Van91I CCANNNNNTGG 1 cut(s) 1632
VpaK11BI GGWCC 2 cut(s) 1489, 1649
XagI CCTNNNNNAGG 1 cut(s) 1472
XapI RAATTY 2 cut(s) 1020, 1082
XceI RCATGY 2 cut(s) 747, 1570
XcmI CCANNNNNNNNNTGG 1 cut(s) 699
XhoI CTCGAG 1 cut(s) 1813
XmaJI CCTAGG 1 cut(s) 749
XmnI GAANNNNTTC 2 cut(s) 1416, 1505
XspI CTAG 2 cut(s) 279, 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.