pycom14g04000

calmodulin-binding family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011034
Physical Location & Seq
Forward (+)
3470699 .. 3472246
1548 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g04000.2

Sequence Viewer

Length: 1068 bp
ATGGGGTTTCCAGTATCCTGCCCACTTGCAGATTTTGATGATTTGGATAGTCATTGTGGAGCTGTTATTGTAAGTCCAGTTAGTCTCAGGAAAGAAGAAGAGAGGAAGCCATTGCAGTCATTGAGCTCCAACACCTGCTGTGATTCGGAACCAACCGCTACAATGAAGTCACCTGGTTCAGGAAATATGAACTTTGAAGAACCAATTAGCTTTAAAGAGATGGAATTAGATGCCGTTTCAGCAAACTCACCTTCCAGGGATGTGGAAAACAATGTGTCTGCTAGAGCAGAAAGCGGTATTGGAGAGAAACAGCAGACATCCGAGAACTCGTTCAAGGAGCCCAGAGACCAGGCTGCATTGAGGCTGCAGAAAGTTTATAAGAGTTTTCGCACGAGAAGACAGCTTGCGGATTGTGCAATTCTTGTGGAGCAGAGATGGTGGAAGGTGCTCGATTTTGCTGAACTAAAGCGGAGCTCTATATCATTTTTCGACATTCAGAAACCTGAGACAGCTGTTTCTCGCTGGTCTCGGGCAAGAACTAAAGCTGCAAAGGTGGGAAAAGGTTTGTCGAAGGATAAAAAAGCACGTAAACTTGCTTTGCAGCATTGGCTTGAGGCTATTGATCCTCGACATCGCTATGGCCACAATCTTCACTTTTACTACGCCAGATGGCTCCATTGCGAAAGTAAAGAACCCTTCTTCTACTGGCTCGATATAGGAGACGGAAGAGAAGTCAGCCATGAAGCATGCCCTAGGTCGAAGCTTCAAACACAATGCATCAAGTATCTTGGCCCGATCGAACGAGAGACTTACGAAATTCTAGTGCAGGATGGGAAGTTCGTGTACAAGCAGAGTGGAAACTTTCTTGACACATCAGAACCTAAGGGCACAAAGTGGATATTTGTGCTGAGTACAACCAAGACATTGTATGTTGGACAGAAGAGCAAGGGGACGTTTCAACATTCGAGTTTCTTGGCAGGCGGAGCAACACTTTCTGCTGGAAGATTAGTAGTGGAACAAGGAATTCTTAAGGTATATAAAAGATCGATCCTTTATGCACTGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

356

Amino Acids

40.27

Weight (kDa)

9.1

Isoelectric Point (pI)

54.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58480
fragaria_vesca FvH4_6g21330 FvH4_6g21330 FvH4_6g21330
malus_domestica MD12G1050400.v1.1 MD14G1048900.v1.1
prunus_persica Prupe.7G086400_v2.0.a1
pyrus_communis pycom12g04490 pycom14g04000
rosa_chinensis RchiOBHm_Chr3g0477941
rosa_laevigata RLG00000023678
rosa_multiflora Rmu_sc0005991.1_g000009 Rmu_sc0028100.1_g000001
rosa_roxburghii Rroxscaffold_6G00403580
rosa_rugosa Rorug03G0164000
rosa_samantha Rh3AG214800 Rh3BG248400 Rh3CG242400 Rh3DG241800
rosa_wichuraiana Rw3G019360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 378
AarI CACCTGC 1 cut(s) 143
Acc36I ACCTGC 1 cut(s) 143
AciI CCGC 5 cut(s) 156, 294, 407, 469, 981
AclWI GGATC 2 cut(s) 617, 1042
AcoI YGGCCR 1 cut(s) 640
AcsI RAATTY 2 cut(s) 816, 1023
AdeI CACNNNGTG 1 cut(s) 894
AfaI GTAC 2 cut(s) 845, 913
AfiI CCNNNNNNNGG 1 cut(s) 179
AflII CTTAAG 1 cut(s) 1028
AgsI TTSAA 4 cut(s) 197, 334, 767, 959
AjnI CCWGG 3 cut(s) 172, 254, 348
AjuI GAANNNNNNNTTGG 2 cut(s) 282, 314
AloI GAACNNNNNNTCC 2 cut(s) 821, 853
AluBI AGCT 8 cut(s) 62, 126, 210, 403, 474, 512, 545, 763
AluI AGCT 8 cut(s) 62, 126, 210, 403, 474, 512, 545, 763
Alw21I GWGCWC 3 cut(s) 128, 450, 476
Alw26I GTCTC 6 cut(s) 89, 339, 500, 531, 714, 800
AlwI GGATC 2 cut(s) 617, 1042
Ama87I CYCGRG 1 cut(s) 528
AoxI GGCC 2 cut(s) 640, 790
ApeKI GCWGC 4 cut(s) 353, 364, 545, 601
ApoI RAATTY 2 cut(s) 816, 1023
Asp700I GAANNNNTTC 1 cut(s) 329
AspA2I CCTAGG 1 cut(s) 752
AspS9I GGNCC 1 cut(s) 791
AsuHPI GGTGA 2 cut(s) 162, 240
AvaI CYCGRG 1 cut(s) 528
AvrII CCTAGG 1 cut(s) 752
AxyI CCTNAGG 1 cut(s) 882
BaeGI GKGCMC 1 cut(s) 890
BalI TGGCCA 1 cut(s) 642
BanII GRGCYC 3 cut(s) 128, 342, 476
BarI GAAGNNNNNNTAC 2 cut(s) 827, 859
BauI CACGAG 1 cut(s) 391
BbsI GAAGAC 1 cut(s) 403
Bbv12I GWGCWC 3 cut(s) 128, 450, 476
BbvI GCAGC 4 cut(s) 340, 351, 532, 613
BccI CCATC 4 cut(s) 214, 429, 663, 824
BceAI ACGGC 1 cut(s) 218
BciT130I CCWGG 3 cut(s) 174, 256, 350
BciVI GTATCC 1 cut(s) 25
BcoDI GTCTC 6 cut(s) 89, 339, 500, 531, 714, 800
BfaI CTAG 3 cut(s) 282, 753, 821
BfmI CTRYAG 1 cut(s) 365
BfrI CTTAAG 1 cut(s) 1028
BfuAI ACCTGC 1 cut(s) 143
BfuI GTATCC 1 cut(s) 25
BisI GCNGC 4 cut(s) 354, 365, 546, 602
BlnI CCTAGG 1 cut(s) 752
BlsI GCNGC 4 cut(s) 355, 366, 547, 603
Bme1390I CCNGG 3 cut(s) 174, 256, 350
BmeT110I CYCGRG 1 cut(s) 528
BmgT120I GGNCC 1 cut(s) 791
BmiI GGNNCC 3 cut(s) 150, 339, 674
BmrFI CCNGG 3 cut(s) 174, 256, 350
BmsI GCATC 2 cut(s) 220, 786
BpiI GAAGAC 1 cut(s) 403
BpuEI CTTGAG 1 cut(s) 632
Bsa29I ATCGAT 1 cut(s) 1046
BsaAI YACGTR 1 cut(s) 587
BsaI GGTCTC 2 cut(s) 339, 531
BsaJI CCNNGG 2 cut(s) 255, 752
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse1I ACTGG 3 cut(s) 11, 77, 710
Bse21I CCTNAGG 1 cut(s) 882
Bse3DI GCAATG 2 cut(s) 110, 676
BseBI CCWGG 3 cut(s) 174, 256, 350
BseCI ATCGAT 1 cut(s) 1046
BseDI CCNNGG 2 cut(s) 255, 752
BseGI GGATG 3 cut(s) 265, 317, 835
BseLI CCNNNNNNNGG 1 cut(s) 179
BseMI GCAATG 2 cut(s) 110, 676
BseMII CTCAG 3 cut(s) 100, 495, 899
BseNI ACTGG 3 cut(s) 11, 77, 710
BseSI GKGCMC 1 cut(s) 890
BseXI GCAGC 4 cut(s) 340, 351, 532, 613
BsgI GTGCAG 1 cut(s) 845
Bsh1285I CGRYCG 1 cut(s) 798
BshFI GGCC 2 cut(s) 642, 792
BshVI ATCGAT 1 cut(s) 1046
BsiEI CGRYCG 1 cut(s) 798
BsiHKAI GWGCWC 3 cut(s) 128, 450, 476
BsiHKCI CYCGRG 1 cut(s) 528
BslFI GGGAC 1 cut(s) 964
BslI CCNNNNNNNGG 1 cut(s) 179
BsmAI GTCTC 6 cut(s) 89, 339, 500, 531, 714, 800
BsmBI CGTCTC 1 cut(s) 714
BsmFI GGGAC 1 cut(s) 964
BsnI GGCC 2 cut(s) 642, 792
Bso31I GGTCTC 2 cut(s) 339, 531
BsoBI CYCGRG 1 cut(s) 528
Bsp1286I GDGCHC 5 cut(s) 128, 342, 450, 476, 890
Bsp1407I TGTACA 1 cut(s) 843
Bsp143I GATC 4 cut(s) 622, 795, 1043, 1047
BspACI CCGC 5 cut(s) 156, 294, 407, 469, 981
BspANI GGCC 2 cut(s) 642, 792
BspCNI CTCAG 3 cut(s) 99, 496, 900
BspDI ATCGAT 1 cut(s) 1046
BspLI GGNNCC 3 cut(s) 150, 339, 674
BspMAI CTGCAG 1 cut(s) 369
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 2 cut(s) 617, 1042
BspQI GCTCTTC 1 cut(s) 935
BspTI CTTAAG 1 cut(s) 1028
BspTNI GGTCTC 2 cut(s) 339, 531
BsrDI GCAATG 2 cut(s) 110, 676
BsrGI TGTACA 1 cut(s) 843
BsrI ACTGG 3 cut(s) 11, 77, 710
BssECI CCNNGG 2 cut(s) 255, 752
BssMI GATC 4 cut(s) 622, 795, 1043, 1047
BssSI CACGAG 1 cut(s) 391
BssT1I CCWWGG 1 cut(s) 752
Bst2BI CACGAG 1 cut(s) 391
Bst2UI CCWGG 3 cut(s) 174, 256, 350
Bst6I CTCTTC 3 cut(s) 93, 721, 935
BstAFI CTTAAG 1 cut(s) 1028
BstAUI TGTACA 1 cut(s) 843
BstBAI YACGTR 1 cut(s) 587
BstC8I GCNNGC 3 cut(s) 405, 748, 979
BstDEI CTNAG 4 cut(s) 86, 504, 882, 908
BstENI CCTNNNNNAGG 1 cut(s) 177
BstF5I GGATG 3 cut(s) 265, 317, 835
BstKTI GATC 4 cut(s) 625, 798, 1046, 1050
BstMAI GTCTC 6 cut(s) 89, 339, 500, 531, 714, 800
BstMBI GATC 4 cut(s) 622, 795, 1043, 1047
BstMCI CGRYCG 1 cut(s) 798
BstMWI GCNNNNNNNGC 4 cut(s) 239, 413, 607, 983
BstNI CCWGG 3 cut(s) 174, 256, 350
BstNSI RCATGY 1 cut(s) 750
BstSCI CCNGG 3 cut(s) 172, 254, 348
BstSFI CTRYAG 1 cut(s) 365
BstSLI GKGCMC 1 cut(s) 890
BstV1I GCAGC 4 cut(s) 340, 351, 532, 613
BstV2I GAAGAC 1 cut(s) 403
BstXI CCANNNNNNTGG 1 cut(s) 262
Bsu15I ATCGAT 1 cut(s) 1046
Bsu36I CCTNAGG 1 cut(s) 882
BsuI GTATCC 1 cut(s) 25
BsuRI GGCC 2 cut(s) 642, 792
BsuTUI ATCGAT 1 cut(s) 1046
BtgZI GCGATG 1 cut(s) 617
BtsCI GGATG 3 cut(s) 265, 317, 835
BtsI GCAGTG 1 cut(s) 1058
BtsIMutI CAGTG 1 cut(s) 1058
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 3 cut(s) 405, 748, 979
Cfr13I GGNCC 1 cut(s) 791
ClaI ATCGAT 1 cut(s) 1046
CsiI ACCWGGT 1 cut(s) 172
Csp6I GTAC 2 cut(s) 844, 912
CspCI CAANNNNNGTGG 4 cut(s) 405, 440, 835, 870
CviAII CATG 2 cut(s) 740, 747
CviQI GTAC 2 cut(s) 844, 912
DdeI CTNAG 4 cut(s) 86, 504, 882, 908
DpnI GATC 4 cut(s) 624, 797, 1045, 1049
DpnII GATC 4 cut(s) 622, 795, 1043, 1047
DraI TTTAAA 1 cut(s) 214
DraIII CACNNNGTG 1 cut(s) 894
EaeI YGGCCR 1 cut(s) 640
Eam1104I CTCTTC 3 cut(s) 93, 721, 935
EarI CTCTTC 3 cut(s) 93, 721, 935
EciI GGCGGA 1 cut(s) 996
Ecl136II GAGCTC 2 cut(s) 126, 474
Eco130I CCWWGG 1 cut(s) 752
Eco24I GRGCYC 3 cut(s) 128, 342, 476
Eco31I GGTCTC 2 cut(s) 339, 531
Eco53kI GAGCTC 2 cut(s) 126, 474
Eco81I CCTNAGG 1 cut(s) 882
Eco88I CYCGRG 1 cut(s) 528
EcoICRI GAGCTC 2 cut(s) 126, 474
EcoNI CCTNNNNNAGG 1 cut(s) 177
EcoRI GAATTC 1 cut(s) 1023
EcoRII CCWGG 3 cut(s) 172, 254, 348
EcoT14I CCWWGG 1 cut(s) 752
EcoT22I ATGCAT 1 cut(s) 779
EcoT38I GRGCYC 3 cut(s) 128, 342, 476
ErhI CCWWGG 1 cut(s) 752
Esp3I CGTCTC 1 cut(s) 714
FaeI CATG 2 cut(s) 743, 750
FalI AAGNNNNNCTT 2 cut(s) 1011, 1043
FaqI GGGAC 1 cut(s) 964
FatI CATG 2 cut(s) 739, 746
Fnu4HI GCNGC 4 cut(s) 354, 365, 546, 602
FokI GGATG 3 cut(s) 272, 304, 842
FriOI GRGCYC 3 cut(s) 128, 342, 476
Fsp4HI GCNGC 4 cut(s) 354, 365, 546, 602
FspBI CTAG 3 cut(s) 282, 753, 821
GluI GCNGC 4 cut(s) 354, 365, 546, 602
HaeIII GGCC 2 cut(s) 642, 792
Hin1II CATG 2 cut(s) 743, 750
HindIII AAGCTT 1 cut(s) 761
HinfI GANTC 1 cut(s) 143
HphI GGTGA 2 cut(s) 162, 240
Hpy166II GTNNAC 2 cut(s) 590, 844
Hpy188I TCNGA 4 cut(s) 148, 322, 498, 877
Hpy188III TCNNGA 3 cut(s) 88, 180, 866
Hpy8I GTNNAC 2 cut(s) 590, 844
HpyAV CCTTC 4 cut(s) 261, 436, 565, 706
HpyCH4IV ACGT 2 cut(s) 586, 953
HpyF10VI GCNNNNNNNGC 4 cut(s) 239, 413, 607, 983
HpyF3I CTNAG 4 cut(s) 86, 504, 882, 908
HpySE526I ACGT 2 cut(s) 586, 953
Hsp92II CATG 2 cut(s) 743, 750
Kzo9I GATC 4 cut(s) 622, 795, 1043, 1047
LguI GCTCTTC 1 cut(s) 935
LmnI GCTCC 7 cut(s) 59, 131, 337, 427, 471, 678, 983
Lsp1109I GCAGC 4 cut(s) 340, 351, 532, 613
LweI GCATC 2 cut(s) 220, 786
MabI ACCWGGT 1 cut(s) 172
MaeI CTAG 3 cut(s) 282, 753, 821
MaeII ACGT 2 cut(s) 586, 953
MaeIII GTNAC 1 cut(s) 168
MalI GATC 4 cut(s) 624, 797, 1045, 1049
MboI GATC 4 cut(s) 622, 795, 1043, 1047
MboII GAAGA 9 cut(s) 107, 110, 209, 408, 641, 691, 738, 952, 1014
MhlI GDGCHC 5 cut(s) 128, 342, 450, 476, 890
MlsI TGGCCA 1 cut(s) 642
MluCI AATT 5 cut(s) 204, 224, 417, 816, 1023
MluNI TGGCCA 1 cut(s) 642
MmeI TCCRAC 2 cut(s) 153, 913
MnlI CCTC 4 cut(s) 96, 354, 607, 636
Mox20I TGGCCA 1 cut(s) 642
Mph1103I ATGCAT 1 cut(s) 779
MroXI GAANNNNTTC 1 cut(s) 329
MscI TGGCCA 1 cut(s) 642
MseI TTAA 2 cut(s) 213, 1029
MslI CAYNNNNRTG 2 cut(s) 636, 1063
Msp20I TGGCCA 1 cut(s) 642
MspA1I CMGCKG 1 cut(s) 512
MspCI CTTAAG 1 cut(s) 1028
MspR9I CCNGG 3 cut(s) 174, 256, 350
MvaI CCWGG 3 cut(s) 174, 256, 350
MwoI GCNNNNNNNGC 4 cut(s) 239, 413, 607, 983
NdeII GATC 4 cut(s) 622, 795, 1043, 1047
NlaIII CATG 2 cut(s) 743, 750
NlaIV GGNNCC 3 cut(s) 150, 339, 674
NmuCI GTSAC 1 cut(s) 168
NsiI ATGCAT 1 cut(s) 779
NspI RCATGY 1 cut(s) 750
PaeI GCATGC 1 cut(s) 750
PaqCI CACCTGC 1 cut(s) 143
PciSI GCTCTTC 1 cut(s) 935
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 1 cut(s) 143
PkrI GCNGC 4 cut(s) 355, 366, 547, 603
Ple19I CGATCG 1 cut(s) 798
Ppu21I YACGTR 1 cut(s) 587
PsiI TTATAA 1 cut(s) 378
Psp124BI GAGCTC 2 cut(s) 128, 476
Psp6I CCWGG 3 cut(s) 172, 254, 348
PspGI CCWGG 3 cut(s) 172, 254, 348
PspN4I GGNNCC 3 cut(s) 150, 339, 674
PspPI GGNCC 1 cut(s) 791
PstI CTGCAG 1 cut(s) 369
PvuI CGATCG 1 cut(s) 798
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 2 cut(s) 845, 913
RsaNI GTAC 2 cut(s) 844, 912
RseI CAYNNNNRTG 2 cut(s) 636, 1063
SacI GAGCTC 2 cut(s) 128, 476
SapI GCTCTTC 1 cut(s) 935
SaqAI TTAA 2 cut(s) 213, 1029
SatI GCNGC 4 cut(s) 354, 365, 546, 602
Sau3AI GATC 4 cut(s) 622, 795, 1043, 1047
Sau96I GGNCC 1 cut(s) 791
ScrFI CCNGG 3 cut(s) 174, 256, 350
SduI GDGCHC 5 cut(s) 128, 342, 450, 476, 890
SexAI ACCWGGT 1 cut(s) 172
SfaNI GCATC 2 cut(s) 220, 786
SfcI CTRYAG 1 cut(s) 365
SmiMI CAYNNNNRTG 2 cut(s) 636, 1063
SmlI CTYRAG 2 cut(s) 611, 1028
SmoI CTYRAG 2 cut(s) 611, 1028
SphI GCATGC 1 cut(s) 750
Sse9I AATT 5 cut(s) 204, 224, 417, 816, 1023
SsiI CCGC 5 cut(s) 156, 294, 407, 469, 981
SspMI CTAG 3 cut(s) 282, 753, 821
SstI GAGCTC 2 cut(s) 128, 476
StyD4I CCNGG 3 cut(s) 172, 254, 348
StyI CCWWGG 1 cut(s) 752
TaiI ACGT 2 cut(s) 589, 956
TaqI TCGA 9 cut(s) 450, 489, 569, 628, 711, 758, 798, 965, 1046
TasI AATT 5 cut(s) 204, 224, 417, 816, 1023
TatI WGTACW 2 cut(s) 843, 911
TfiI GAWTC 1 cut(s) 143
Tru1I TTAA 2 cut(s) 213, 1029
Tru9I TTAA 2 cut(s) 213, 1029
TscAI CASTG 1 cut(s) 1065
TseFI GTSAC 1 cut(s) 168
TseI GCWGC 4 cut(s) 353, 364, 545, 601
Tsp45I GTSAC 1 cut(s) 168
TspDTI ATGAA 3 cut(s) 179, 203, 756
TspGWI ACGGA 1 cut(s) 738
TspRI CASTG 1 cut(s) 1065
Vha464I CTTAAG 1 cut(s) 1028
XagI CCTNNNNNAGG 1 cut(s) 177
XapI RAATTY 2 cut(s) 816, 1023
XceI RCATGY 1 cut(s) 750
XmaJI CCTAGG 1 cut(s) 752
XmnI GAANNNNTTC 1 cut(s) 329
XspI CTAG 3 cut(s) 282, 753, 821
Zsp2I ATGCAT 1 cut(s) 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.