pycom12g05400

Rhodanese-like domain-containing protein 9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012197
Physical Location & Seq
Forward (+)
5256868 .. 5258666
1799 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 717 bp
ATGGCAGGGATTGGCTGTTGCTGTACAACTCTCTCGTCCTCAAGCAATCTCTACACATCTTGGTTGAAATTTGAGACTCGTACTCATGGTGGAGGAACCATCTTAGGGCAACCAACCCGTCGTCGTCGGAATTTCTCAATCAGAGCAGAAGTGAATTTTGTAAGTGCAGAAGAAGCAAAAGAACTTATTGCAGTCGAGGGATATACTGTCATTGATGTCCGGGACAAATCTCAGTACGAACGAGCTCATATCAAATCTTGCCATCATATGCCTCTCTTCATTGAAAACCAAGACAATGATTTTGGAACAATCATAAAGAGGACTCTCCACAACAATTTTTCTGGTCTCTTCTATGGGTTGCCTTTTACCAAACCGAACCCTGAATTTGTAGAGTCAGTAAAAAACCGGTTCTCACCTGAAAGCAAACTGTTACTGGTGTGTCAGGAAGGATTGAGGTCTACTGCTGCTGCCGATAAGTTGGAGCAAGCGGGTTTCGAAAACATAGCGTGTGTAACTTCGGGTCTTCAAAGCGTAAAACCAGGAGCATTTGATTCTGTTGGTTCGACGGAGTTGCAAAATGCCGGCAAGGCCGGATTAGTCACTATTCAGGGAAAGATCTCAGCTGTACTTGGAACTGTGCTAATCTGTGCATATTTGTTCATCACCTTCTTCCCCGATCAAGCAGAGAAGCTACTCGCAACTTTCCCGACCACCTAG

Protein Analysis

239

Amino Acids

26.08

Weight (kDa)

7.59

Isoelectric Point (pI)

49.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 55 - 177 2.8e-11 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012197)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42220
fragaria_vesca FvH4_6g22700 FvH4_6g22700 FvH4_6g22700
malus_domestica MD12G1061100.v1.1 MD14G1060800.v1.1
prunus_persica Prupe.7G073600_v2.0.a1 Prupe.7G073600_v2.0.a1
pyrus_communis pycom12g05400
rosa_chinensis RchiOBHm_Chr3g0480311
rosa_laevigata RLG00000023493
rosa_multiflora Rmu_ssc0000353.1_g000001
rosa_roxburghii Rroxscaffold_6G00401570
rosa_rugosa Rorug03G0182400
rosa_samantha Rh3AG234300 Rh3BG266600 Rh3CG264100 Rh3DG259600
rosa_wichuraiana Rw3G021030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 458
AciI CCGC 1 cut(s) 488
AcsI RAATTY 4 cut(s) 68, 130, 154, 383
AfaI GTAC 4 cut(s) 25, 82, 236, 627
AfiI CCNNNNNNNGG 1 cut(s) 105
AgeI ACCGGT 1 cut(s) 405
AgsI TTSAA 3 cut(s) 67, 284, 527
AjnI CCWGG 1 cut(s) 538
AluBI AGCT 3 cut(s) 245, 623, 691
AluI AGCT 3 cut(s) 245, 623, 691
Alw21I GWGCWC 1 cut(s) 247
Alw26I GTCTC 2 cut(s) 68, 350
AoxI GGCC 1 cut(s) 588
ApeKI GCWGC 2 cut(s) 464, 467
ApoI RAATTY 4 cut(s) 68, 130, 154, 383
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
AsiGI ACCGGT 1 cut(s) 405
AsuC2I CCSGG 1 cut(s) 221
AsuHPI GGTGA 2 cut(s) 405, 655
AsuII TTCGAA 1 cut(s) 495
BanII GRGCYC 1 cut(s) 247
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 1 cut(s) 247
BbvI GCAGC 2 cut(s) 451, 454
BccI CCATC 2 cut(s) 107, 270
BcgI CGANNNNNNTGC 2 cut(s) 553, 587
BciT130I CCWGG 1 cut(s) 540
BcnI CCSGG 1 cut(s) 221
BcoDI GTCTC 2 cut(s) 68, 350
BfaI CTAG 1 cut(s) 715
BglI GCCNNNNNGGC 1 cut(s) 587
BglII AGATCT 1 cut(s) 615
BisI GCNGC 2 cut(s) 465, 468
BlsI GCNGC 2 cut(s) 466, 469
Bme1390I CCNGG 2 cut(s) 221, 540
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 2 cut(s) 221, 540
BpiI GAAGAC 1 cut(s) 515
Bpu14I TTCGAA 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 25
BpuMI CCSGG 1 cut(s) 221
BsaI GGTCTC 1 cut(s) 350
BsaWI WCCGGW 1 cut(s) 405
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse118I RCCGGY 2 cut(s) 405, 581
Bse1I ACTGG 1 cut(s) 438
BseBI CCWGG 1 cut(s) 540
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMII CTCAG 2 cut(s) 245, 633
BseNI ACTGG 1 cut(s) 438
BseXI GCAGC 2 cut(s) 451, 454
BsgI GTGCAG 1 cut(s) 186
BshFI GGCC 1 cut(s) 590
BshTI ACCGGT 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 247
BsiSI CCGG 4 cut(s) 220, 406, 582, 591
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 1 cut(s) 105
BsmAI GTCTC 2 cut(s) 68, 350
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 1 cut(s) 590
Bso31I GGTCTC 1 cut(s) 350
Bsp119I TTCGAA 1 cut(s) 495
Bsp1286I GDGCHC 1 cut(s) 247
Bsp1407I TGTACA 1 cut(s) 23
Bsp143I GATC 2 cut(s) 615, 676
BspACI CCGC 1 cut(s) 488
BspANI GGCC 1 cut(s) 590
BspCNI CTCAG 2 cut(s) 244, 632
BspLI GGNNCC 1 cut(s) 97
BspT104I TTCGAA 1 cut(s) 495
BspTNI GGTCTC 1 cut(s) 350
BsrFI RCCGGY 2 cut(s) 405, 581
BsrGI TGTACA 1 cut(s) 23
BsrI ACTGG 1 cut(s) 438
BssAI RCCGGY 2 cut(s) 405, 581
BssMI GATC 2 cut(s) 615, 676
Bst2UI CCWGG 1 cut(s) 540
Bst4CI ACNGT 3 cut(s) 208, 429, 637
Bst6I CTCTTC 2 cut(s) 281, 353
BstAUI TGTACA 1 cut(s) 23
BstBI TTCGAA 1 cut(s) 495
BstC8I GCNNGC 2 cut(s) 486, 583
BstDEI CTNAG 3 cut(s) 103, 231, 619
BstKTI GATC 2 cut(s) 618, 679
BstMAI GTCTC 2 cut(s) 68, 350
BstMBI GATC 2 cut(s) 615, 676
BstMWI GCNNNNNNNGC 2 cut(s) 173, 587
BstNI CCWGG 1 cut(s) 540
BstSCI CCNGG 2 cut(s) 219, 538
BstV1I GCAGC 2 cut(s) 451, 454
BstV2I GAAGAC 1 cut(s) 515
BstX2I RGATCY 1 cut(s) 615
BstYI RGATCY 1 cut(s) 615
BsuRI GGCC 1 cut(s) 590
Cac8I GCNNGC 2 cut(s) 486, 583
Cfr10I RCCGGY 2 cut(s) 405, 581
Csp6I GTAC 4 cut(s) 24, 81, 235, 626
CspAI ACCGGT 1 cut(s) 405
CviAII CATG 1 cut(s) 86
CviJI RGCY 5 cut(s) 15, 245, 590, 623, 691
CviKI_1 RGCY 5 cut(s) 15, 245, 590, 623, 691
CviQI GTAC 4 cut(s) 24, 81, 235, 626
DdeI CTNAG 3 cut(s) 103, 231, 619
DpnI GATC 2 cut(s) 617, 678
DpnII GATC 2 cut(s) 615, 676
Eam1104I CTCTTC 2 cut(s) 281, 353
EarI CTCTTC 2 cut(s) 281, 353
Ecl136II GAGCTC 1 cut(s) 245
Eco24I GRGCYC 1 cut(s) 247
Eco31I GGTCTC 1 cut(s) 350
Eco53kI GAGCTC 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 538
EcoT38I GRGCYC 1 cut(s) 247
FaeI CATG 1 cut(s) 89
FaiI YATR 9 cut(s) 87, 204, 249, 267, 269, 314, 354, 503, 652
FaqI GGGAC 1 cut(s) 236
FatI CATG 1 cut(s) 85
FauI CCCGC 1 cut(s) 481
FauNDI CATATG 1 cut(s) 267
FblI GTMKAC 1 cut(s) 458
Fnu4HI GCNGC 2 cut(s) 465, 468
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 2 cut(s) 465, 468
FspBI CTAG 1 cut(s) 715
GluI GCNGC 2 cut(s) 465, 468
HaeIII GGCC 1 cut(s) 590
HapII CCGG 4 cut(s) 220, 406, 582, 591
Hin1II CATG 1 cut(s) 89
HinfI GANTC 4 cut(s) 76, 322, 392, 551
HpaII CCGG 4 cut(s) 220, 406, 582, 591
HphI GGTGA 2 cut(s) 405, 655
Hpy166II GTNNAC 1 cut(s) 459
Hpy188I TCNGA 2 cut(s) 129, 143
Hpy188III TCNNGA 2 cut(s) 443, 706
Hpy8I GTNNAC 1 cut(s) 459
Hpy99I CGWCG 4 cut(s) 123, 126, 129, 568
HpyAV CCTTC 2 cut(s) 440, 676
HpyCH4III ACNGT 3 cut(s) 208, 429, 637
HpyCH4V TGCA 4 cut(s) 167, 191, 574, 650
HpyF10VI GCNNNNNNNGC 2 cut(s) 173, 587
HpyF3I CTNAG 3 cut(s) 103, 231, 619
Hsp92II CATG 1 cut(s) 89
KroI GCCGGC 1 cut(s) 581
KroNI GCCGGC 1 cut(s) 583
Kzo9I GATC 2 cut(s) 615, 676
LmnI GCTCC 2 cut(s) 481, 542
Lsp1109I GCAGC 2 cut(s) 451, 454
MaeI CTAG 1 cut(s) 715
MaeIII GTNAC 3 cut(s) 429, 511, 598
MalI GATC 2 cut(s) 617, 678
MboI GATC 2 cut(s) 615, 676
MboII GAAGA 5 cut(s) 182, 268, 340, 515, 661
MflI RGATCY 1 cut(s) 615
MhlI GDGCHC 1 cut(s) 247
MluCI AATT 5 cut(s) 68, 130, 154, 334, 383
MlyI GAGTC 3 cut(s) 70, 316, 401
MmeI TCCRAC 2 cut(s) 107, 459
MnlI CCTC 6 cut(s) 49, 86, 190, 282, 312, 447
MroNI GCCGGC 1 cut(s) 581
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 4 cut(s) 220, 406, 582, 591
MspR9I CCNGG 2 cut(s) 221, 540
MvaI CCWGG 1 cut(s) 540
MwoI GCNNNNNNNGC 2 cut(s) 173, 587
NaeI GCCGGC 1 cut(s) 583
NciI CCSGG 1 cut(s) 221
NdeI CATATG 1 cut(s) 267
NdeII GATC 2 cut(s) 615, 676
NgoMIV GCCGGC 1 cut(s) 581
NlaIII CATG 1 cut(s) 89
NlaIV GGNNCC 1 cut(s) 97
NmuCI GTSAC 1 cut(s) 598
NspV TTCGAA 1 cut(s) 495
PdiI GCCGGC 1 cut(s) 583
PfeI GAWTC 1 cut(s) 551
PfoI TCCNGGA 1 cut(s) 219
PinAI ACCGGT 1 cut(s) 405
PkrI GCNGC 2 cut(s) 466, 469
PleI GAGTC 3 cut(s) 70, 316, 400
PpsI GAGTC 3 cut(s) 70, 316, 400
Psp124BI GAGCTC 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 538
PspGI CCWGG 1 cut(s) 538
PspN4I GGNNCC 1 cut(s) 97
PsuI RGATCY 1 cut(s) 615
PvuII CAGCTG 1 cut(s) 623
RsaI GTAC 4 cut(s) 25, 82, 236, 627
RsaNI GTAC 4 cut(s) 24, 81, 235, 626
SacI GAGCTC 1 cut(s) 247
SatI GCNGC 2 cut(s) 465, 468
Sau3AI GATC 2 cut(s) 615, 676
SchI GAGTC 3 cut(s) 70, 316, 401
ScrFI CCNGG 2 cut(s) 221, 540
SduI GDGCHC 1 cut(s) 247
SetI ASST 7 cut(s) 247, 418, 458, 625, 668, 693, 716
SfuI TTCGAA 1 cut(s) 495
SmlI CTYRAG 1 cut(s) 40
SmoI CTYRAG 1 cut(s) 40
Sse9I AATT 5 cut(s) 68, 130, 154, 334, 383
SsiI CCGC 1 cut(s) 488
SspMI CTAG 1 cut(s) 715
SstI GAGCTC 1 cut(s) 247
StyD4I CCNGG 2 cut(s) 219, 538
TaaI ACNGT 3 cut(s) 208, 429, 637
TaqI TCGA 3 cut(s) 195, 495, 563
TasI AATT 5 cut(s) 68, 130, 154, 334, 383
TatI WGTACW 2 cut(s) 23, 625
TfiI GAWTC 1 cut(s) 551
TseFI GTSAC 1 cut(s) 598
TseI GCWGC 2 cut(s) 464, 467
Tsp45I GTSAC 1 cut(s) 598
TspDTI ATGAA 2 cut(s) 268, 649
TspGWI ACGGA 1 cut(s) 581
XapI RAATTY 4 cut(s) 68, 130, 154, 383
XmiI GTMKAC 1 cut(s) 458
XspI CTAG 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.