pycom12g06390

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013576
Physical Location & Seq
Forward (+)
6517650 .. 6521171
3522 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1392 bp
ATGGCGTGCAGTGCTTCAGGTTGCGAATCAAATTGCTACAAAACCCAACAAGAAGAAGATGAAGATGTCAAAGACAACAACAATTACAAATCAATTTCTACAAACACTAGAAACAACAACAGCAACAGTAATTTGTGCCTCAAGTGCAAGGCCAATCAACCCATCTCTTCCAACACCGACGGCGGCGGCGACGATGCGCGCTTCTGTGCCGATTGCTTCCGCGGCAATCTCTTCGGGAAGTTCAGGTTCGCTGTCACCTCCAACGCCATGATTTCTCCTACTGACAATGTCCTCGTTGCCTTCTCCGGTGGCCCTTCTTCCAGGGTTGCTTTGCAGTTTGTACATGAGATGCATAATAAAGCACAGAAGAATTTCGATGCAAGTAGGGATAGATCGTTGCCAGTTTTTGGGGTTGGAGTTGCATTTGTTGATGAGAGTTCTGTTTATTCTGTTCCGTCTGGTGAAGTAGAAAACGCAATTGAAGATATTAGATTGATCGTGGAAGACCTAGCTCCACCAACAAAAGAACTGCATGTTGTACCAATTGAAAGTGTATACTCTTCAGATTCTGGTGATGGAAGGGGTAGATTAAATCAGTTGTTAGAAGCTGTTCCAGATGCTACTGGGAAAGAAGATCTTTTGCTTCACATGCGGATGTTGGCATTGCAAAAGGTTGCCTCTGAAAAGGGATACAACAGACTAGTGCTCGGATCATGCATATCGAGGATCGCTTGCCATGTTATTACAGCCACTGTCAAGGGCCAAGGATATTCTTTACCAGCTGATATACAATATGTTGATGCAAGGTGGGAGATCCCAGTGTTACTTCCTCTTCGTGATTGTCTTGCACGAGAGCTGACCATGCTTTGCTGCCTTGATGGTCTAAAAACTGTGGAGTTGATGAGAAGTCCTAGTACTGGAATAAACGGCTTGGTCTCATCATTCGTGACACTTTTGCAGGAAGAAAATCCTTCTCGAGAGTGCACAATTGTGAGAACTGCTGGAAAGCTTATCCCATTTCATTTTAACGGAATTCAAGAGATAAATGACTCTAAGGTTTCTTTGGAAACTCGAAGGCGTCTGAAGAGATATAACGTTAAACCTAATGAATCTTTTTCCTCAGAGTCATTCTGCTTTATCTGCAATAGCCCACTCAGCCGATCTGACTTGCTAAGTTTGAAGAATTTTGAGGACCACAAAACAAGTTGTGTCTGCTGTTTAAGCTGCCAGTTTCAGATACTTCCTGAAGACCCCTCATCAATAAATCAATTTTTTACTCTTTTTCCTCAGCAGCTGGCTGCTCGCGCAAAGCATGACAATCTTGACCATAACAGTGTCCTCAGGGAACAAATACAAGATTGCTTGCTCTCAGAAGGTGAAGATGAGACTTGA

Protein Analysis

464

Amino Acids

51.2

Weight (kDa)

5.29

Isoelectric Point (pI)

53.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013576)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35910
fragaria_vesca FvH4_6g23820
malus_domestica MD12G1074000.v1.1
prunus_persica Prupe.7G060900_v2.0.a1
pyrus_communis pycom12g06390
rosa_chinensis RchiOBHm_Chr3g0482381
rosa_laevigata RLG00000023353 RLG00000023355 RLG00000023356
rosa_multiflora Rmu_sc0008293.1_g000002 Rmu_sc0013521.1_g000004
rosa_roxburghii Rroxscaffold_6G00399370
rosa_rugosa Rorug03G0195900
rosa_samantha Rh3AG247900 Rh3BG282400 Rh3CG279400
rosa_wichuraiana Rw3G022220 Rw3G022300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 407
AccI GTMKAC 1 cut(s) 555
AccII CGCG 3 cut(s) 199, 222, 1305
AciI CCGC 5 cut(s) 183, 186, 220, 222, 652
AclI AACGTT 1 cut(s) 1095
AclWI GGATC 3 cut(s) 718, 734, 808
AcsI RAATTY 3 cut(s) 370, 1032, 1183
AcuI CTGAAG 3 cut(s) 546, 1103, 1266
AcyI GRCGYC 1 cut(s) 1078
AfaI GTAC 3 cut(s) 342, 540, 916
AfiI CCNNNNNNNGG 2 cut(s) 407, 917
AgsI TTSAA 4 cut(s) 482, 548, 1037, 1180
AhlI ACTAGT 1 cut(s) 700
AjnI CCWGG 1 cut(s) 320
AleI CACNNNNGTG 1 cut(s) 989
AluBI AGCT 7 cut(s) 512, 608, 782, 856, 1009, 1224, 1294
AluI AGCT 7 cut(s) 512, 608, 782, 856, 1009, 1224, 1294
Alw21I GWGCWC 2 cut(s) 708, 986
Alw26I GTCTC 2 cut(s) 940, 1379
Alw44I GTGCAC 1 cut(s) 982
AlwI GGATC 3 cut(s) 718, 734, 808
AlwNI CAGNNNCTG 3 cut(s) 569, 752, 1294
Ama87I CYCGRG 1 cut(s) 975
AoxI GGCC 3 cut(s) 150, 310, 760
ApaLI GTGCAC 1 cut(s) 982
ApeKI GCWGC 4 cut(s) 870, 1224, 1291, 1298
ApoI RAATTY 3 cut(s) 370, 1032, 1183
Asp700I GAANNNNTTC 2 cut(s) 371, 609
AspLEI GCGC 3 cut(s) 199, 201, 1307
AspS9I GGNCC 3 cut(s) 311, 760, 1192
AsuHPI GGTGA 4 cut(s) 247, 473, 584, 1388
AvaI CYCGRG 1 cut(s) 975
AvaII GGWCC 1 cut(s) 1192
AxyI CCTNAGG 1 cut(s) 1340
BaeGI GKGCMC 1 cut(s) 986
BarI GAAGNNNNNNTAC 2 cut(s) 898, 930
BauI CACGAG 1 cut(s) 849
BbsI GAAGAC 2 cut(s) 510, 1254
Bbv12I GWGCWC 2 cut(s) 708, 986
BbvCI CCTCAGC 1 cut(s) 1287
BbvI GCAGC 4 cut(s) 857, 1211, 1285, 1303
BccI CCATC 3 cut(s) 170, 569, 872
BceAI ACGGC 2 cut(s) 196, 943
BcgI CGANNNNNNTGC 2 cut(s) 214, 248
BciT130I CCWGG 1 cut(s) 322
BciVI GTATCC 1 cut(s) 683
BcoDI GTCTC 2 cut(s) 940, 1379
BcuI ACTAGT 1 cut(s) 700
BfaI CTAG 4 cut(s) 108, 509, 701, 912
BfuI GTATCC 1 cut(s) 683
BglII AGATCT 1 cut(s) 634
BisI GCNGC 7 cut(s) 184, 187, 223, 871, 1225, 1292, 1299
BlsI GCNGC 7 cut(s) 185, 188, 224, 872, 1226, 1293, 1300
BmcAI AGTACT 1 cut(s) 916
Bme1390I CCNGG 1 cut(s) 322
Bme18I GGWCC 1 cut(s) 1192
BmeT110I CYCGRG 1 cut(s) 975
BmgT120I GGNCC 3 cut(s) 311, 760, 1192
BmrFI CCNGG 1 cut(s) 322
BmrI ACTGGG 2 cut(s) 633, 812
BmsI GCATC 5 cut(s) 184, 339, 367, 607, 790
BmuI ACTGGG 2 cut(s) 633, 812
BpiI GAAGAC 2 cut(s) 510, 1254
Bpu10I CCTNAGC 1 cut(s) 1287
BpuEI CTTGAG 1 cut(s) 125
BsaHI GRCGYC 1 cut(s) 1078
BsaI GGTCTC 1 cut(s) 940
BsaJI CCNNGG 3 cut(s) 220, 321, 763
BsaWI WCCGGW 1 cut(s) 305
Bsc4I CCNNNNNNNGG 2 cut(s) 407, 917
Bse1I ACTGG 5 cut(s) 401, 628, 818, 922, 1228
Bse21I CCTNAGG 1 cut(s) 1340
Bse3DI GCAATG 1 cut(s) 662
BseBI CCWGG 1 cut(s) 322
BseDI CCNNGG 3 cut(s) 220, 321, 763
BseGI GGATG 1 cut(s) 660
BseLI CCNNNNNNNGG 2 cut(s) 407, 917
BseMI GCAATG 1 cut(s) 662
BseMII CTCAG 5 cut(s) 1134, 1168, 1301, 1354, 1383
BseNI ACTGG 5 cut(s) 401, 628, 818, 922, 1228
BsePI GCGCGC 1 cut(s) 197
BseSI GKGCMC 1 cut(s) 986
BseXI GCAGC 4 cut(s) 857, 1211, 1285, 1303
BsgI GTGCAG 1 cut(s) 28
Bsh1236I CGCG 3 cut(s) 199, 222, 1305
BshFI GGCC 3 cut(s) 152, 312, 762
BsiHKAI GWGCWC 2 cut(s) 708, 986
BsiHKCI CYCGRG 1 cut(s) 975
BsiSI CCGG 1 cut(s) 306
BslI CCNNNNNNNGG 2 cut(s) 407, 917
BsmAI GTCTC 2 cut(s) 940, 1379
BsnI GGCC 3 cut(s) 152, 312, 762
Bso31I GGTCTC 1 cut(s) 940
BsoBI CYCGRG 1 cut(s) 975
Bsp1286I GDGCHC 2 cut(s) 708, 986
Bsp1407I TGTACA 1 cut(s) 340
Bsp143I GATC 7 cut(s) 392, 495, 634, 710, 726, 813, 1160
BspACI CCGC 5 cut(s) 183, 186, 220, 222, 652
BspANI GGCC 3 cut(s) 152, 312, 762
BspCNI CTCAG 5 cut(s) 1133, 1167, 1300, 1353, 1382
BspFNI CGCG 3 cut(s) 199, 222, 1305
BspPI GGATC 3 cut(s) 718, 734, 808
BspTNI GGTCTC 1 cut(s) 940
BsrDI GCAATG 1 cut(s) 662
BsrGI TGTACA 1 cut(s) 340
BsrI ACTGG 5 cut(s) 401, 628, 818, 922, 1228
BssECI CCNNGG 3 cut(s) 220, 321, 763
BssHII GCGCGC 1 cut(s) 197
BssMI GATC 7 cut(s) 392, 495, 634, 710, 726, 813, 1160
BssNAI GTATAC 1 cut(s) 556
BssNI GRCGYC 1 cut(s) 1078
BssSI CACGAG 1 cut(s) 849
BssT1I CCWWGG 1 cut(s) 763
Bst1107I GTATAC 1 cut(s) 556
Bst2BI CACGAG 1 cut(s) 849
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 4 cut(s) 128, 754, 892, 1334
Bst6I CTCTTC 5 cut(s) 172, 236, 565, 837, 1079
BstACI GRCGYC 1 cut(s) 1078
BstAUI TGTACA 1 cut(s) 340
BstC8I GCNNGC 6 cut(s) 7, 199, 733, 1296, 1303, 1364
BstDEI CTNAG 7 cut(s) 1053, 1120, 1154, 1172, 1287, 1340, 1369
BstDSI CCRYGG 1 cut(s) 220
BstF5I GGATG 1 cut(s) 660
BstFNI CGCG 3 cut(s) 199, 222, 1305
BstHHI GCGC 3 cut(s) 199, 201, 1307
BstKTI GATC 7 cut(s) 395, 498, 637, 713, 729, 816, 1163
BstMAI GTCTC 2 cut(s) 940, 1379
BstMBI GATC 7 cut(s) 392, 495, 634, 710, 726, 813, 1160
BstMWI GCNNNNNNNGC 9 cut(s) 11, 144, 222, 649, 862, 1140, 1155, 1221, 1304
BstNI CCWGG 1 cut(s) 322
BstNSI RCATGY 2 cut(s) 536, 652
BstSCI CCNGG 1 cut(s) 320
BstSLI GKGCMC 1 cut(s) 986
BstUI CGCG 3 cut(s) 199, 222, 1305
BstV1I GCAGC 4 cut(s) 857, 1211, 1285, 1303
BstV2I GAAGAC 2 cut(s) 510, 1254
BstX2I RGATCY 2 cut(s) 634, 813
BstYI RGATCY 2 cut(s) 634, 813
BstZ17I GTATAC 1 cut(s) 556
Bsu36I CCTNAGG 1 cut(s) 1340
BsuI GTATCC 1 cut(s) 683
BsuRI GGCC 3 cut(s) 152, 312, 762
BtgI CCRYGG 1 cut(s) 220
BtsCI GGATG 1 cut(s) 660
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 4 cut(s) 16, 750, 825, 1339
Cac8I GCNNGC 6 cut(s) 7, 199, 733, 1296, 1303, 1364
CaiI CAGNNNCTG 3 cut(s) 569, 752, 1294
CfoI GCGC 3 cut(s) 199, 201, 1307
Cfr13I GGNCC 3 cut(s) 311, 760, 1192
Cfr42I CCGCGG 1 cut(s) 223
CseI GACGC 1 cut(s) 1067
Csp6I GTAC 3 cut(s) 341, 539, 915
CviAII CATG 8 cut(s) 268, 344, 533, 649, 714, 737, 862, 1313
CviQI GTAC 3 cut(s) 341, 539, 915
DdeI CTNAG 7 cut(s) 1053, 1120, 1154, 1172, 1287, 1340, 1369
DpnI GATC 7 cut(s) 394, 497, 636, 712, 728, 815, 1162
DpnII GATC 7 cut(s) 392, 495, 634, 710, 726, 813, 1160
Eam1104I CTCTTC 5 cut(s) 172, 236, 565, 837, 1079
EarI CTCTTC 5 cut(s) 172, 236, 565, 837, 1079
Eco130I CCWWGG 1 cut(s) 763
Eco31I GGTCTC 1 cut(s) 940
Eco47I GGWCC 1 cut(s) 1192
Eco57I CTGAAG 3 cut(s) 546, 1103, 1266
Eco81I CCTNAGG 1 cut(s) 1340
Eco88I CYCGRG 1 cut(s) 975
EcoRI GAATTC 1 cut(s) 1032
EcoRII CCWGG 1 cut(s) 320
EcoT14I CCWWGG 1 cut(s) 763
EcoT22I ATGCAT 2 cut(s) 354, 719
ErhI CCWWGG 1 cut(s) 763
FaeI CATG 8 cut(s) 271, 347, 536, 652, 717, 740, 865, 1316
FalI AAGNNNNNCTT 2 cut(s) 621, 653
FatI CATG 8 cut(s) 267, 343, 532, 648, 713, 736, 861, 1312
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 7 cut(s) 184, 187, 223, 871, 1225, 1292, 1299
FokI GGATG 1 cut(s) 667
Fsp4HI GCNGC 7 cut(s) 184, 187, 223, 871, 1225, 1292, 1299
FspBI CTAG 4 cut(s) 108, 509, 701, 912
GlaI GCGC 3 cut(s) 198, 200, 1306
GluI GCNGC 7 cut(s) 184, 187, 223, 871, 1225, 1292, 1299
HaeIII GGCC 3 cut(s) 152, 312, 762
HapII CCGG 1 cut(s) 306
HgaI GACGC 1 cut(s) 1067
HhaI GCGC 3 cut(s) 199, 201, 1307
Hin1I GRCGYC 1 cut(s) 1078
Hin1II CATG 8 cut(s) 271, 347, 536, 652, 717, 740, 865, 1316
Hin6I GCGC 3 cut(s) 197, 199, 1305
HinP1I GCGC 3 cut(s) 197, 199, 1305
HindIII AAGCTT 1 cut(s) 1007
HinfI GANTC 5 cut(s) 26, 566, 1049, 1109, 1124
HpaII CCGG 1 cut(s) 306
HphI GGTGA 4 cut(s) 247, 473, 584, 1388
Hpy166II GTNNAC 2 cut(s) 556, 984
Hpy188I TCNGA 8 cut(s) 565, 682, 710, 1083, 1123, 1165, 1236, 1372
Hpy188III TCNNGA 9 cut(s) 235, 614, 836, 946, 975, 977, 1037, 1244, 1322
Hpy8I GTNNAC 2 cut(s) 556, 984
Hpy99I CGWCG 2 cut(s) 182, 194
HpyAV CCTTC 6 cut(s) 310, 324, 573, 981, 1068, 1367
HpyCH4III ACNGT 4 cut(s) 128, 754, 892, 1334
HpyCH4IV ACGT 1 cut(s) 1095
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 144, 222, 649, 862, 1140, 1155, 1221, 1304
HpyF3I CTNAG 7 cut(s) 1053, 1120, 1154, 1172, 1287, 1340, 1369
HpySE526I ACGT 1 cut(s) 1095
Hsp92I GRCGYC 1 cut(s) 1078
Hsp92II CATG 8 cut(s) 271, 347, 536, 652, 717, 740, 865, 1316
HspAI GCGC 3 cut(s) 197, 199, 1305
KspI CCGCGG 1 cut(s) 223
Kzo9I GATC 7 cut(s) 392, 495, 634, 710, 726, 813, 1160
LmnI GCTCC 1 cut(s) 517
Lsp1109I GCAGC 4 cut(s) 857, 1211, 1285, 1303
LweI GCATC 5 cut(s) 184, 339, 367, 607, 790
MaeI CTAG 4 cut(s) 108, 509, 701, 912
MaeII ACGT 1 cut(s) 1095
MaeIII GTNAC 3 cut(s) 253, 822, 946
MalI GATC 7 cut(s) 394, 497, 636, 712, 728, 815, 1162
MboI GATC 7 cut(s) 392, 495, 634, 710, 726, 813, 1160
MfeI CAATTG 3 cut(s) 477, 543, 987
MflI RGATCY 2 cut(s) 634, 813
MhlI GDGCHC 2 cut(s) 708, 986
MlyI GAGTC 2 cut(s) 1043, 1133
MmeI TCCRAC 3 cut(s) 195, 285, 394
Mph1103I ATGCAT 2 cut(s) 354, 719
MroXI GAANNNNTTC 2 cut(s) 371, 609
MseI TTAA 4 cut(s) 590, 1026, 1098, 1220
MslI CAYNNNNRTG 3 cut(s) 653, 989, 1332
MspA1I CMGCKG 3 cut(s) 222, 782, 1294
MspI CCGG 1 cut(s) 306
MspR9I CCNGG 1 cut(s) 322
MunI CAATTG 3 cut(s) 477, 543, 987
MvaI CCWGG 1 cut(s) 322
MvnI CGCG 3 cut(s) 199, 222, 1305
MwoI GCNNNNNNNGC 9 cut(s) 11, 144, 222, 649, 862, 1140, 1155, 1221, 1304
NdeII GATC 7 cut(s) 392, 495, 634, 710, 726, 813, 1160
NlaIII CATG 8 cut(s) 271, 347, 536, 652, 717, 740, 865, 1316
NmuCI GTSAC 2 cut(s) 253, 946
NsiI ATGCAT 2 cut(s) 354, 719
NspI RCATGY 2 cut(s) 536, 652
OliI CACNNNNGTG 1 cut(s) 989
PaeR7I CTCGAG 1 cut(s) 975
PauI GCGCGC 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PdmI GAANNNNTTC 2 cut(s) 371, 609
PfeI GAWTC 3 cut(s) 26, 566, 1109
PflFI GACNNNGTC 1 cut(s) 287
PflMI CCANNNNNTGG 1 cut(s) 407
PkrI GCNGC 7 cut(s) 185, 188, 224, 872, 1226, 1293, 1300
PleI GAGTC 2 cut(s) 1043, 1132
PpsI GAGTC 2 cut(s) 1043, 1132
Psp1406I AACGTT 1 cut(s) 1095
Psp6I CCWGG 1 cut(s) 320
PspGI CCWGG 1 cut(s) 320
PspPI GGNCC 3 cut(s) 311, 760, 1192
PstNI CAGNNNCTG 3 cut(s) 569, 752, 1294
PsuI RGATCY 2 cut(s) 634, 813
PsyI GACNNNGTC 1 cut(s) 287
PteI GCGCGC 1 cut(s) 197
PvuII CAGCTG 2 cut(s) 782, 1294
RsaI GTAC 3 cut(s) 342, 540, 916
RsaNI GTAC 3 cut(s) 341, 539, 915
RseI CAYNNNNRTG 3 cut(s) 653, 989, 1332
SacII CCGCGG 1 cut(s) 223
SaqAI TTAA 4 cut(s) 590, 1026, 1098, 1220
SatI GCNGC 7 cut(s) 184, 187, 223, 871, 1225, 1292, 1299
Sau3AI GATC 7 cut(s) 392, 495, 634, 710, 726, 813, 1160
Sau96I GGNCC 3 cut(s) 311, 760, 1192
ScaI AGTACT 1 cut(s) 916
SchI GAGTC 2 cut(s) 1043, 1133
ScrFI CCNGG 1 cut(s) 322
SduI GDGCHC 2 cut(s) 708, 986
SfaNI GCATC 5 cut(s) 184, 339, 367, 607, 790
Sfr274I CTCGAG 1 cut(s) 975
Sfr303I CCGCGG 1 cut(s) 223
SgrBI CCGCGG 1 cut(s) 223
SinI GGWCC 1 cut(s) 1192
SlaI CTCGAG 1 cut(s) 975
SmiMI CAYNNNNRTG 3 cut(s) 653, 989, 1332
SmlI CTYRAG 2 cut(s) 140, 975
SmoI CTYRAG 2 cut(s) 140, 975
SpeI ACTAGT 1 cut(s) 700
SsiI CCGC 5 cut(s) 183, 186, 220, 222, 652
SspMI CTAG 4 cut(s) 108, 509, 701, 912
StyD4I CCNGG 1 cut(s) 320
StyI CCWWGG 1 cut(s) 763
TaaI ACNGT 4 cut(s) 128, 754, 892, 1334
TaiI ACGT 1 cut(s) 1098
TaqI TCGA 4 cut(s) 375, 722, 976, 1072
TatI WGTACW 2 cut(s) 340, 914
TauI GCSGC 3 cut(s) 186, 189, 225
TfiI GAWTC 3 cut(s) 26, 566, 1109
Tru1I TTAA 4 cut(s) 590, 1026, 1098, 1220
Tru9I TTAA 4 cut(s) 590, 1026, 1098, 1220
TscAI CASTG 4 cut(s) 16, 757, 825, 1339
TseFI GTSAC 2 cut(s) 253, 946
TseI GCWGC 4 cut(s) 870, 1224, 1291, 1298
Tsp45I GTSAC 2 cut(s) 253, 946
TspDTI ATGAA 3 cut(s) 75, 1010, 1122
TspGWI ACGGA 2 cut(s) 444, 1044
TspRI CASTG 4 cut(s) 16, 757, 825, 1339
Tth111I GACNNNGTC 1 cut(s) 287
Van91I CCANNNNNTGG 1 cut(s) 407
VneI GTGCAC 1 cut(s) 982
VpaK11BI GGWCC 1 cut(s) 1192
XapI RAATTY 3 cut(s) 370, 1032, 1183
XceI RCATGY 2 cut(s) 536, 652
XhoI CTCGAG 1 cut(s) 975
XmiI GTMKAC 1 cut(s) 555
XmnI GAANNNNTTC 2 cut(s) 371, 609
XspI CTAG 4 cut(s) 108, 509, 701, 912
ZrmI AGTACT 1 cut(s) 916
Zsp2I ATGCAT 2 cut(s) 354, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.