pycom12g09080

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009494
Physical Location & Seq
Reverse (-)
10859068 .. 10867715
8648 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4950 bp
ATGGGCAAAGCTGTTGTCTCTGACGAAGAAGATGAGGTCGAGCTCGACGAGGACGACAGGGAACCTCTTCACGGCGATGCCGTCGATGACCAGGATGGTGGCGACGAGGAGGAGGACGAGGAAGAAGAAGGGCCAGATGAATATGAGAAGGATGATTTTATAGTAGATGATGTAGACGAAGACGATGAGCAAGAGGAGGAAGAAGAGAGAGCAGACAGTGATGAAGAACGGCAGAGGAAGAAAAAAAGAAAGAAAAAAGAGGAGTATGTCCTTGATGAAGATGACTATGAGCTCCTTGAGGACAATAATGTAATTGCACCCCGAAGGAAGGCTGGGAAATTTAAGCGTCTGAAAAAAGCTCAGAGGCATGGTGTGGGGGAGCCTGGTGAATTTTCTGACGAGGAGGAGTTTGATGGTAGTGGTAAAGGTGGAAGAACTGCAGAGGAGAAACTTGAGCGCAGCTTATTTGGTCATGATGAAGGTACTCCATTTGAGGATATCGTTGAAGAGGAGGAACCAGAAGAAGCAGAAGATGATGGAGAAGTTGGTGAAGAGGATGAAATGGCAGACTTTATAGTCGATGAAGAGTTTGATGAGACTGGGACTCTTGTGAGACAGAGGAAGCTTAAGAGAAAAAAGTCTAGGCAGGCACCAGGAGTATCATCAGATGCTTTGCAGGAAGCTCACGATATATTTGGTGATGTTGATGAATTACTACAGCTCCGTAAGCAAGGTTTGGATTCCAGTGAATGGAGGGAAAGGAAGCTCGAGGATGAATTTGAACCAATTATTCTTTCTGAAAAGTATATGACAGAGAAGGATGACCAGATTCGGGAGCTTGATGTTCCAGAAAGAGTGCAGGTATACGAGGAAAGCACTGGTTCTTTTCCACTGGATGGAATTAGTATAGACGATGAGAGTACCTGGATATATGATCAAATTGCAAGTGGTACAATACCTTTGTTTAGCAAGCCAGGCTTGGCGAATTCTATAAGCAGGGAAGATATCAACAGATTTTTGGACTTGCATCATGTGCAGAAGCTGGACATTCCTTATATTGCTATGTATCGGAAAGAGGATTGCCTTAGTTTATTGAAAGATCCTGAACATCTTGAATTGGAAGATGTAAATCAGAATGAAAATGAGAAATCGTCTGGGCTAAAGTGGCACAAGGTACTTTGGAATATTCAGGACTTGGACAGAAAATGGCTTCTGCTTCAGAAACGAAAAAGTGCTCTGCAATCTTACTACAATAAGCGATTTGAGGAAGAATCCCGACGTATATATGATGAGAGCAGACTTACATTGAATCAACAACTTTTTGAGTCAATCATGAAGTCGCTCAAGGCTGCAGAATCAGAGAGAGAGGTTGATGATGTTGACACCAAGTTTAACTTGCATTTCCCTCCTGGTGAGGCTGGAGTGGATGAGGGACAATATAAGAGGCCCAAGAGGAAATCTCTTTACAGTGTCTGCAGTAAGGCTGGCCTGTGGGAAGTTGCAAGCAAATTTGGTTACAGCTCTGAGCAGTTTGGACTGCAATTATCTCTAGAAAAAATGAGAATGGATGAGTTGGAGGATGCGAAGGAGACACCGGAGGAGATGGCGTCGAACTTCACATGTGCAATGTTTGAGACACCTCAAGCTGTGCTCAAAGGGGCTAGGCATATGGCAGCTGTTGAGATAAGTTGTGAGCCATGTGTCAGGAAGTATGTTCGATGCAACTACTTGGATACCGTTGAGTTATCGACAAGCCCCACACCCGCCGGAAATGCGGCAATAGATGCTTTTCATCAGTTCTCTGGCGTGAAGTGGTTGCAAAGAAAGCCATTAAATAAATTTGAGGATGCACAATGGCTGCTTATCCAGAAGGCTGAGGAGGAGAAGCTTCTTCAGGTTACTATTAAGTTGCCAGAAGACAGACTAAATAAGTTGATAAGCGACTTTAATGAGTACTATCTTAGTGATGGTGTTAGTAAGTCTGCACAACTGTGGAATGAGCAACGGAAGTTGATATTACAGGATGCTCTTTTTAGTTTTCTTCTTCCTTCAATGGAGAAAGAAGCAAGATCGATGTTGACCAGTAGAGCAAAAAACTGGTTACTGATGGAATATGGAAAGGTTTTGTGGAATAAAGTCTCAGTGAGACCATACCAAAGGAAGGAAAATGATAATAGCTCAGACGATGAACCTGCACCTAGGGTCATGGCTTGCTGCTGGGGCCCTGGGAAGCCAGCAACCACTTTTGTGATGTTAGATTCATCTGGGGAGATTCTGGATGTACTTTACACGGGGTCCCTCACTTTACGCTCTCATAATGTTAACGATCAGCAACGAAAGAAAAATGATCAAGAACGTGTTCTGAAGTTCATGACTGATCATCAACCACAAGTCGCTGTCCTAGGAGCTGTTAACTTGTCTTGTGTTCGCCTAAAAGATGATATTTATGAGATAATTTTTAAGATGGTGGAGGAAAATCCTAGAGATGTTGGCCATGATATGGACGGTTTAAGCATTGTATATGGGGATGAATCTTTGGCCCGCCTGTATGAAAATTCTCGGATTTCATCTGATCAGCTACCTGCACAACAAGGAATTGTCAAGAGGGCTGTGGCTCTTGGGCGTAATCTTCAAAATCCATTGGCAATGGTTGCCACACTCTGTGGACCAGGAAGAGAAATCTTATCTTGGAAACTCAATCCTTTTGAGAACTTTCTCACCCCAGATGAGAAGTATGTGATGGTTGAACAGGTCATGGTAGATGTCACGAACCAGGTAGGCCTTGATATTAATTTGGCTATAAGCCATGAATGGTTATTTGCACCTTTGCAATTCATTTCTGGGCTTGGACCTAGAAAGGCGGCATCTTTGCAGAGATCGTTGGTAAGATCTGGTGCAATATTTACTCGAAAGGATTTGGTGAACCCACACGGGCTTGGTAGAAAGGTATTTGTTAATGCATCCGGTTTCTTACGTGTTCGGCGGAGTGGATTGGCTGCTAGCAGCAGCCAATATATTGATCTGTTGGATGATACGAGAATTCATCCAGAATACTATATAATTGCGCAAGACTTGGCCAAAGATGTTTATGATGTGGATGGTAACAACGATGATGAGGATTTGGAGATGGCTATAGAACACGTCCGGGATCGACCTAGTTATCTAAAAAACCTTGATGTTGAAGCTTATGCTAAAAGTAAAAAGCTTGAGAATAAGATACAAACATTTTATGATATAAGGAGAGAATTAATACAGGGTTTTCAGGATTGGCGTAAACAATATGAAGAACCAAGCCAGGATGAAGAATTCTATATGATTTCAGGTGAAACCGAGGATACCCTTGCTGAAGGAAGAATTGTACAGGCTACAGTTCGCAGGGTGCAAGGTCAGAGGGCAATTTGTGCTCTTGAATCTGGATTAACTGGCATGCTTATGAAAGAAGATTATTCAGATGATTCAAGAGATATGGAATTGTCTGACAGACTGAATGAGGGCGACATTCTCACTTGCAAGATCAAATCCATTCAAAAGAATAGGTACCAAGTTTTCCTAAGTTGTAGAGAGAGTGAGATGAGAAATAACCGTTATCAGAACACTCAGAATCTTGATACCTATTACCATGAAGACCGAAGAAGTCTACAGAGTGAGCAAGATAAAGCTCATAAGGAGAAAGAGCTTGCAAAGAAGCATTTCAAGCCAAGGATGATTGTTCATCCTCGCTTCCAAAATATAACTGCAGATGAAGCAATGAAGTTCTTGTCTGATAAGGATCCTGGGGAAAGTATCATTCGTCCCAGTTCCCGTGGGCCTTCGTATTTAACCTTGACTCTCAAGGTTTATGATGGAGTATATGCTCACAAAGACATTGTTGAAGGTGGAAAGGATCATAAGGATATAACGAGCTTACTCCGCATTGGGAAGACTTTGAAAATTGGGGAGGACACTTTTGAGGATTTGGATGAGGTAATGGATCGTTATGTTGATCCCTTAGTAGCTCACTTGAAGGCAATTCTAAATTATCGCAAGTTCAGGAAGGGAACAAAACAAGAGGTCGATGAACTTTTGAAGATTGAGAAATTGGAGTATCCTATGAGGATAGTTTATTCTTTTGGGATATCGCATGAACATCCAGGCACTTTCATCCTGACCTATATACGGAGTACAAATCCACACCATGAGTATGTTGGTCTATATCCGAAGGGATTCAAATTCAGAAAAAGGATGTTTGAAGACATTGATCGGCTTTTAGCATATTTTCAGCGCCATATTGATGACCCTCAGCATGAGTCAGGACCATCTATTAGATCAGTCGCTGCTATGGTGCCGATGCGAAGCCCTGCAGCTGGGGGTTCTTCAGGGGCATCTGTGGGCAGTGGCTGGGGTGGATCAACTAATGAGGGTGGTTGGAGAGGCCAGTCTTTTGATGGGGATCGGTCATCTACACCGAGTTCACGAACAGGTCGAAGTGATCATAGAAATGGTGGTAGTCGGGATGGGCATCCAAGCGGCCTACCTAGACCATATGGTGGGCGTGGTCGTGGTCGAGGAGCATATAACAACAGAGGAAATAGCACTGGCAATGAGAGGCAGGATTCTGGTTATGATGCTCCTGCGTGGGGTTCAGATTCCAAGGATGGGGATGATGGTTTGGGCAAATTTCCAGGTGCAAAGGTCCAGAACTCACCTGGAAGGGAAGCTTTCCCTGGTGGTTGGGGTGCTGGTGGAAGTGGGAGTGGAGGTGGTGGCAGTAGCTGGGGCGGTGGAGCTGGCTCCAGTGGAAATGATGCATGGGGCGGTGCTGCTGATAATGAGACTTCAGGATGGGGTTCTGACACCAAGAAAAAAGGTTCCGGTGATGGAGATTTGGGATGGGGTTCTGAACCGAAAAGAAGTACCCAGTCTCAGTCTGGAAACAGATGGTCTGGAACTGACGGAGGTGGCGGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

1650

Amino Acids

186.89

Weight (kDa)

4.95

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT6_acidic PF14632 35 - 122 6.7e-15 Acidic N-terminal SPT6
HTH_44 PF14641 303 - 419 7.9e-06 Helix-turn-helix DNA-binding domain of SPT6
Tex_central_region PF22706 528 - 697 7.7e-06 Tex central region-like
YqgF PF14639 733 - 888 4.8e-15 Holliday-junction resolvase-like of SPT6
HHH_7 PF14635 892 - 993 2.8e-21 Helix-hairpin-helix motif
Spt6_S1 PF21710 1103 - 1190 5e-11 Spt6, S1/OB domain
SH2_2 PF14633 1206 - 1422 7.8e-69 SH2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 575
Acc16I TGCGCA 1 cut(s) 3066
Acc36I ACCTGC 3 cut(s) 850, 2200, 2590
Acc65I GGTACC 1 cut(s) 3537
AccB1I GGYRCC 3 cut(s) 649, 3537, 4321
AccB7I CCANNNNNTGG 4 cut(s) 750, 896, 2500, 4526
AccI GTMKAC 3 cut(s) 174, 864, 3637
AclWI GGATC 9 cut(s) 1094, 3156, 3764, 3777, 3891, 3977, 3978, 4393, 4437
AcoI YGGCCR 2 cut(s) 2491, 3075
AcuI CTGAAG 6 cut(s) 1202, 1877, 2384, 3366, 4338, 4800
AcyI GRCGYC 1 cut(s) 1607
AdeI CACNNNGTG 1 cut(s) 2663
AflII CTTAAG 1 cut(s) 626
AflIII ACRYGT 4 cut(s) 1619, 2356, 2974, 3139
AjiI CACGTC 1 cut(s) 3142
AjuI GAANNNNNNNTTGG 2 cut(s) 778, 810
AleI CACNNNNGTG 2 cut(s) 1990, 2246
Alw21I GWGCWC 5 cut(s) 45, 294, 1237, 1653, 3406
Alw26I GTCTC 9 cut(s) 22, 590, 607, 1583, 1628, 2140, 2143, 4805, 4905
AlwI GGATC 9 cut(s) 1094, 3156, 3764, 3777, 3891, 3977, 3978, 4393, 4437
AlwNI CAGNNNCTG 5 cut(s) 1042, 1473, 4313, 4377, 4752
Ama87I CYCGRG 1 cut(s) 767
ApaI GGGCCC 1 cut(s) 2225
ArsI GACNNNNNNTTYG 1 cut(s) 32
AseI ATTAAT 2 cut(s) 2790, 3248
Asp700I GAANNNNTTC 4 cut(s) 66, 2358, 3689, 4202
Asp718I GGTACC 1 cut(s) 3537
AspA2I CCTAGG 2 cut(s) 2198, 2401
AspLEI GCGC 3 cut(s) 459, 3067, 4263
AsuC2I CCSGG 1 cut(s) 3146
AsuHPI GGTGA 9 cut(s) 398, 560, 710, 1424, 2710, 2932, 3335, 4674, 4865
AsuNHI GCTAGC 1 cut(s) 2999
AvaI CYCGRG 1 cut(s) 767
AvaII GGWCC 5 cut(s) 2295, 2666, 2849, 4292, 4672
AvrII CCTAGG 2 cut(s) 2198, 2401
BaeGI GKGCMC 1 cut(s) 2225
BalI TGGCCA 2 cut(s) 2493, 3077
BamHI GGATCC 1 cut(s) 3769
BanI GGYRCC 3 cut(s) 649, 3537, 4321
BanII GRGCYC 3 cut(s) 45, 294, 2225
BbsI GAAGAC 5 cut(s) 186, 1923, 3630, 3926, 4236
Bbv12I GWGCWC 5 cut(s) 45, 294, 1237, 1653, 3406
BbvCI CCTCAGC 2 cut(s) 1875, 4278
BceAI ACGGC 3 cut(s) 65, 88, 245
BcgI CGANNNNNNTGC 2 cut(s) 2174, 2208
BciVI GTATCC 3 cut(s) 1726, 3328, 4095
BclI TGATCA 5 cut(s) 934, 2347, 2377, 2572, 4468
BcnI CCSGG 1 cut(s) 3146
BcoDI GTCTC 9 cut(s) 22, 590, 607, 1583, 1628, 2140, 2143, 4805, 4905
BfmI CTRYAG 9 cut(s) 438, 716, 1350, 1474, 3132, 3366, 3638, 3735, 4338
BfoI RGCGCY 1 cut(s) 4264
BfrI CTTAAG 1 cut(s) 626
BfuAI ACCTGC 3 cut(s) 850, 2200, 2590
BfuI GTATCC 3 cut(s) 1726, 3328, 4095
BglII AGATCT 1 cut(s) 2888
BlnI CCTAGG 2 cut(s) 2198, 2401
BmcAI AGTACT 1 cut(s) 1955
Bme18I GGWCC 5 cut(s) 2295, 2666, 2849, 4292, 4672
BmeT110I CYCGRG 1 cut(s) 767
BmgBI CACGTC 1 cut(s) 3142
BmrI ACTGGG 3 cut(s) 609, 3789, 4891
BmtI GCTAGC 1 cut(s) 3003
BmuI ACTGGG 3 cut(s) 609, 3789, 4891
BpiI GAAGAC 5 cut(s) 186, 1923, 3630, 3926, 4236
BplI GAGNNNNNCTC 2 cut(s) 1444, 1476
BpmI CTGGAG 2 cut(s) 1440, 4756
Bpu10I CCTNAGC 2 cut(s) 1875, 4278
BpuEI CTTGAG 6 cut(s) 317, 473, 1328, 1626, 3227, 3815
BpuMI CCSGG 1 cut(s) 3146
Bsa29I ATCGAT 1 cut(s) 2072
BsaAI YACGTR 1 cut(s) 2975
BsaBI GATNNNNATC 4 cut(s) 1128, 3768, 4428, 4497
BsaHI GRCGYC 1 cut(s) 1607
BsaI GGTCTC 1 cut(s) 2140
BsaWI WCCGGW 3 cut(s) 1594, 2963, 4850
BsaXI ACNNNNNCTCC 6 cut(s) 705, 735, 1592, 1622, 4723, 4753
Bse3DI GCAATG 4 cut(s) 1632, 2652, 3753, 4585
Bse8I GATNNNNATC 4 cut(s) 1128, 3768, 4428, 4497
BseCI ATCGAT 1 cut(s) 2072
BseJI GATNNNNATC 4 cut(s) 1128, 3768, 4428, 4497
BseMI GCAATG 4 cut(s) 1632, 2652, 3753, 4585
BseMII CTCAG 8 cut(s) 374, 1515, 1866, 2154, 2193, 3611, 4292, 4916
BseSI GKGCMC 1 cut(s) 2225
BseYI CCCAGC 5 cut(s) 332, 2217, 4343, 4377, 4752
BsgI GTGCAG 5 cut(s) 878, 1055, 1968, 2178, 2568
BshNI GGYRCC 3 cut(s) 649, 3537, 4321
BshVI ATCGAT 1 cut(s) 2072
BsiHKAI GWGCWC 5 cut(s) 45, 294, 1237, 1653, 3406
BsiHKCI CYCGRG 1 cut(s) 767
BsiSI CCGG 5 cut(s) 1595, 1767, 2964, 3145, 4851
BslFI GGGAC 4 cut(s) 616, 1446, 2281, 3777
BsmAI GTCTC 9 cut(s) 22, 590, 607, 1583, 1628, 2140, 2143, 4805, 4905
BsmFI GGGAC 4 cut(s) 616, 1446, 2281, 3777
Bso31I GGTCTC 1 cut(s) 2140
BsoBI CYCGRG 1 cut(s) 767
Bsp120I GGGCCC 1 cut(s) 2221
Bsp1286I GDGCHC 6 cut(s) 45, 294, 1237, 1653, 2225, 3406
Bsp1407I TGTACA 1 cut(s) 3358
BspCNI CTCAG 8 cut(s) 373, 1516, 1867, 2153, 2192, 3610, 4291, 4915
BspDI ATCGAT 1 cut(s) 2072
BspHI TCATGA 3 cut(s) 472, 1332, 2370
BspMAI CTGCAG 5 cut(s) 442, 1354, 1478, 3739, 4342
BspMI ACCTGC 3 cut(s) 850, 2200, 2590
BspOI GCTAGC 1 cut(s) 3003
BspPI GGATC 9 cut(s) 1094, 3156, 3764, 3777, 3891, 3977, 3978, 4393, 4437
BspT107I GGYRCC 3 cut(s) 649, 3537, 4321
BspTI CTTAAG 1 cut(s) 626
BspTNI GGTCTC 1 cut(s) 2140
BsrDI GCAATG 4 cut(s) 1632, 2652, 3753, 4585
BsrGI TGTACA 1 cut(s) 3358
BssNAI GTATAC 1 cut(s) 865
BssNI GRCGYC 1 cut(s) 1607
BssT1I CCWWGG 4 cut(s) 2198, 2401, 3698, 4629
Bst1107I GTATAC 1 cut(s) 865
Bst4CI ACNGT 7 cut(s) 218, 1469, 1738, 1992, 2507, 3370, 3584
Bst6I CTCTTC 6 cut(s) 72, 198, 501, 546, 579, 2668
BstACI GRCGYC 1 cut(s) 1607
BstAFI CTTAAG 1 cut(s) 626
BstAPI GCANNNNNTGC 4 cut(s) 1033, 1784, 2651, 3401
BstAUI TGTACA 1 cut(s) 3358
BstBAI YACGTR 1 cut(s) 2975
BstDSI CCRYGG 1 cut(s) 3802
BstH2I RGCGCY 1 cut(s) 4264
BstHHI GCGC 3 cut(s) 459, 3067, 4263
BstMAI GTCTC 9 cut(s) 22, 590, 607, 1583, 1628, 2140, 2143, 4805, 4905
BstNSI RCATGY 2 cut(s) 1623, 3430
BstSFI CTRYAG 9 cut(s) 438, 716, 1350, 1474, 3132, 3366, 3638, 3735, 4338
BstSLI GKGCMC 1 cut(s) 2225
BstV2I GAAGAC 5 cut(s) 186, 1923, 3630, 3926, 4236
BstX2I RGATCY 3 cut(s) 1099, 2888, 3769
BstXI CCANNNNNNTGG 1 cut(s) 98
BstYI RGATCY 3 cut(s) 1099, 2888, 3769
BstZ17I GTATAC 1 cut(s) 865
Bsu15I ATCGAT 1 cut(s) 2072
BsuI GTATCC 3 cut(s) 1726, 3328, 4095
BsuTUI ATCGAT 1 cut(s) 2072
BtgI CCRYGG 1 cut(s) 3802
BtgZI GCGATG 1 cut(s) 90
BtrI CACGTC 1 cut(s) 3142
BtsI GCAGTG 1 cut(s) 4378
BtsIMutI CAGTG 9 cut(s) 223, 751, 876, 890, 1474, 2148, 4378, 4572, 4780
BveI ACCTGC 3 cut(s) 850, 2200, 2590
CaiI CAGNNNCTG 5 cut(s) 1042, 1473, 4313, 4377, 4752
CciI TCATGA 3 cut(s) 472, 1332, 2370
CfoI GCGC 3 cut(s) 459, 3067, 4263
ClaI ATCGAT 1 cut(s) 2072
CseI GACGC 2 cut(s) 335, 1596
CsiI ACCWGGT 1 cut(s) 2772
DraIII CACNNNGTG 1 cut(s) 2663
DrdI GACNNNNNNGTC 1 cut(s) 575
DseDI GACNNNNNNGTC 1 cut(s) 575
EaeI YGGCCR 2 cut(s) 2491, 3075
Eam1104I CTCTTC 6 cut(s) 72, 198, 501, 546, 579, 2668
EarI CTCTTC 6 cut(s) 72, 198, 501, 546, 579, 2668
EciI GGCGGA 1 cut(s) 2998
Ecl136II GAGCTC 2 cut(s) 43, 292
Eco130I CCWWGG 4 cut(s) 2198, 2401, 3698, 4629
Eco147I AGGCCT 1 cut(s) 2781
Eco24I GRGCYC 3 cut(s) 45, 294, 2225
Eco31I GGTCTC 1 cut(s) 2140
Eco32I GATATC 3 cut(s) 499, 1006, 4116
Eco47I GGWCC 5 cut(s) 2295, 2666, 2849, 4292, 4672
Eco53kI GAGCTC 2 cut(s) 43, 292
Eco57I CTGAAG 6 cut(s) 1202, 1877, 2384, 3366, 4338, 4800
Eco88I CYCGRG 1 cut(s) 767
EcoICRI GAGCTC 2 cut(s) 43, 292
EcoO109I RGGNCCY 3 cut(s) 2221, 2222, 2295
EcoRI GAATTC 3 cut(s) 985, 3039, 3305
EcoRV GATATC 3 cut(s) 499, 1006, 4116
EcoT14I CCWWGG 4 cut(s) 2198, 2401, 3698, 4629
EcoT22I ATGCAT 2 cut(s) 2962, 4789
EcoT38I GRGCYC 3 cut(s) 45, 294, 2225
ErhI CCWWGG 4 cut(s) 2198, 2401, 3698, 4629
FalI AAGNNNNNCTT 6 cut(s) 962, 994, 1379, 1411, 4681, 4713
FaqI GGGAC 4 cut(s) 616, 1446, 2281, 3777
FauI CCCGC 2 cut(s) 1771, 2549
FauNDI CATATG 2 cut(s) 1668, 4522
FbaI TGATCA 5 cut(s) 934, 2347, 2377, 2572, 4468
FblI GTMKAC 3 cut(s) 174, 864, 3637
FriOI GRGCYC 3 cut(s) 45, 294, 2225
FspI TGCGCA 1 cut(s) 3066
GlaI GCGC 3 cut(s) 458, 3066, 4262
GsaI CCCAGC 5 cut(s) 336, 2221, 4347, 4381, 4756
GsuI CTGGAG 2 cut(s) 1440, 4756
HaeII RGCGCY 1 cut(s) 4264
HapII CCGG 5 cut(s) 1595, 1767, 2964, 3145, 4851
HgaI GACGC 2 cut(s) 335, 1596
HhaI GCGC 3 cut(s) 459, 3067, 4263
Hin1I GRCGYC 1 cut(s) 1607
Hin6I GCGC 3 cut(s) 457, 3065, 4261
HinP1I GCGC 3 cut(s) 457, 3065, 4261
HincII GTYRAC 4 cut(s) 1381, 2079, 2323, 2413
HindII GTYRAC 4 cut(s) 1381, 2079, 2323, 2413
HindIII AAGCTT 5 cut(s) 623, 1886, 3183, 3203, 4695
HpaI GTTAAC 2 cut(s) 2323, 2413
HpaII CCGG 5 cut(s) 1595, 1767, 2964, 3145, 4851
HphI GGTGA 9 cut(s) 398, 560, 710, 1424, 2710, 2932, 3335, 4674, 4865
Hpy99I CGWCG 5 cut(s) 50, 86, 107, 1281, 1612
HpyCH4III ACNGT 7 cut(s) 218, 1469, 1738, 1992, 2507, 3370, 3584
HpyCH4IV ACGT 4 cut(s) 1279, 2356, 2974, 3141
HpySE526I ACGT 4 cut(s) 1279, 2356, 2974, 3141
Hsp92I GRCGYC 1 cut(s) 1607
HspAI GCGC 3 cut(s) 457, 3065, 4261
KflI GGGWCCC 1 cut(s) 2295
KpnI GGTACC 1 cut(s) 3541
Ksp22I TGATCA 5 cut(s) 934, 2347, 2377, 2572, 4468
KspAI GTTAAC 2 cut(s) 2323, 2413
LmnI GCTCC 9 cut(s) 297, 379, 726, 835, 2405, 4547, 4612, 4763, 4775
MabI ACCWGGT 1 cut(s) 2772
MaeII ACGT 4 cut(s) 1279, 2356, 2974, 3141
MaeIII GTNAC 5 cut(s) 1514, 1897, 2100, 2764, 3101
MflI RGATCY 3 cut(s) 1099, 2888, 3769
MhlI GDGCHC 6 cut(s) 45, 294, 1237, 1653, 2225, 3406
MlsI TGGCCA 2 cut(s) 2493, 3077
MluNI TGGCCA 2 cut(s) 2493, 3077
MlyI GAGTC 4 cut(s) 598, 1334, 3820, 4295
MmeI TCCRAC 3 cut(s) 1554, 3006, 4385
Mox20I TGGCCA 2 cut(s) 2493, 3077
Mph1103I ATGCAT 2 cut(s) 2962, 4789
MroXI GAANNNNTTC 4 cut(s) 66, 2358, 3689, 4202
MscI TGGCCA 2 cut(s) 2493, 3077
MslI CAYNNNNRTG 7 cut(s) 75, 1990, 2246, 3431, 4179, 4269, 4476
Msp20I TGGCCA 2 cut(s) 2493, 3077
MspA1I CMGCKG 2 cut(s) 1676, 4343
MspCI CTTAAG 1 cut(s) 626
MspI CCGG 5 cut(s) 1595, 1767, 2964, 3145, 4851
NciI CCSGG 1 cut(s) 3146
NdeI CATATG 2 cut(s) 1668, 4522
NheI GCTAGC 1 cut(s) 2999
NmuCI GTSAC 1 cut(s) 2764
NsbI TGCGCA 1 cut(s) 3066
NsiI ATGCAT 2 cut(s) 2962, 4789
NspI RCATGY 2 cut(s) 1623, 3430
OliI CACNNNNGTG 2 cut(s) 1990, 2246
PaeI GCATGC 1 cut(s) 3430
PaeR7I CTCGAG 1 cut(s) 767
PagI TCATGA 3 cut(s) 472, 1332, 2370
PasI CCCWGGG 1 cut(s) 2225
PceI AGGCCT 1 cut(s) 2781
PciI ACATGT 1 cut(s) 1619
PcsI WCGNNNNNNNCGW 4 cut(s) 45, 51, 78, 4459
PdmI GAANNNNTTC 4 cut(s) 66, 2358, 3689, 4202
PflMI CCANNNNNTGG 4 cut(s) 750, 896, 2500, 4526
PfoI TCCNGGA 1 cut(s) 3144
PleI GAGTC 4 cut(s) 598, 1333, 3820, 4294
PpsI GAGTC 4 cut(s) 598, 1333, 3820, 4294
Ppu21I YACGTR 1 cut(s) 2975
PpuMI RGGWCCY 1 cut(s) 2295
PscI ACATGT 1 cut(s) 1619
PshBI ATTAAT 2 cut(s) 2790, 3248
Psp124BI GAGCTC 2 cut(s) 45, 294
Psp5II RGGWCCY 1 cut(s) 2295
PspFI CCCAGC 5 cut(s) 332, 2217, 4343, 4377, 4752
PspOMI GGGCCC 1 cut(s) 2221
PspPPI RGGWCCY 1 cut(s) 2295
PspXI VCTCGAGB 1 cut(s) 767
PstI CTGCAG 5 cut(s) 442, 1354, 1478, 3739, 4342
PstNI CAGNNNCTG 5 cut(s) 1042, 1473, 4313, 4377, 4752
PsuI RGATCY 3 cut(s) 1099, 2888, 3769
PvuII CAGCTG 2 cut(s) 1676, 4343
RseI CAYNNNNRTG 7 cut(s) 75, 1990, 2246, 3431, 4179, 4269, 4476
SacI GAGCTC 2 cut(s) 45, 294
ScaI AGTACT 1 cut(s) 1955
SchI GAGTC 4 cut(s) 598, 1334, 3820, 4295
SduI GDGCHC 6 cut(s) 45, 294, 1237, 1653, 2225, 3406
SexAI ACCWGGT 1 cut(s) 2772
SfcI CTRYAG 9 cut(s) 438, 716, 1350, 1474, 3132, 3366, 3638, 3735, 4338
Sfr274I CTCGAG 1 cut(s) 767
SinI GGWCC 5 cut(s) 2295, 2666, 2849, 4292, 4672
SlaI CTCGAG 1 cut(s) 767
SmiMI CAYNNNNRTG 7 cut(s) 75, 1990, 2246, 3431, 4179, 4269, 4476
SmlI CTYRAG 8 cut(s) 296, 452, 626, 767, 1343, 1641, 3206, 3830
SmoI CTYRAG 8 cut(s) 296, 452, 626, 767, 1343, 1641, 3206, 3830
SphI GCATGC 1 cut(s) 3430
SseBI AGGCCT 1 cut(s) 2781
SspI AATATT 2 cut(s) 1186, 2901
SstI GAGCTC 2 cut(s) 45, 294
StuI AGGCCT 1 cut(s) 2781
StyI CCWWGG 4 cut(s) 2198, 2401, 3698, 4629
TaaI ACNGT 7 cut(s) 218, 1469, 1738, 1992, 2507, 3370, 3584
TaiI ACGT 4 cut(s) 1282, 2359, 2977, 3144
TaqII GACCGA 2 cut(s) 3642, 4422
TatI WGTACW 4 cut(s) 1953, 2281, 3358, 4160
TauI GCSGC 3 cut(s) 1778, 2864, 4509
TscAI CASTG 9 cut(s) 223, 751, 883, 897, 1474, 2148, 4378, 4579, 4780
TseFI GTSAC 1 cut(s) 2764
Tsp45I GTSAC 1 cut(s) 2764
TspGWI ACGGA 4 cut(s) 713, 2020, 4171, 4947
TspRI CASTG 9 cut(s) 223, 751, 883, 897, 1474, 2148, 4378, 4579, 4780
Van91I CCANNNNNTGG 4 cut(s) 750, 896, 2500, 4526
Vha464I CTTAAG 1 cut(s) 626
VpaK11BI GGWCC 5 cut(s) 2295, 2666, 2849, 4292, 4672
VspI ATTAAT 2 cut(s) 2790, 3248
XbaI TCTAGA 1 cut(s) 1549
XceI RCATGY 2 cut(s) 1623, 3430
XcmI CCANNNNNNNNNTGG 4 cut(s) 4181, 4421, 4682, 4904
XhoI CTCGAG 1 cut(s) 767
XmaJI CCTAGG 2 cut(s) 2198, 2401
XmiI GTMKAC 3 cut(s) 174, 864, 3637
XmnI GAANNNNTTC 4 cut(s) 66, 2358, 3689, 4202
ZrmI AGTACT 1 cut(s) 1955
Zsp2I ATGCAT 2 cut(s) 2962, 4789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.