pycom12g15020

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012720
Physical Location & Seq
Reverse (-)
17443704 .. 17445414
1711 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1065 bp
ATGCGTGAGATTCTTCATATCCAAGGTGGCCAGTGCGGCAACCAGATCGGCGCCAAGTTCTGGGAGGTGGTCTGCGCCGAGCACGGCATCGACGGCACCGGAAGGTACGAGGGAGACGCGGAGCTCCAGCTTGAGAGGATCAATGTTTACTACAACGAAGCCAGTTGCGGGAGGTACGTGCCGAGGGCTGTGCTCATGGACCTTGAGCCTGGGACCATGGACAGCGTCAGATCTGGACCCTACGGTCAGATCTTCAGGCCTGATAACTTCGTTTTCGGCCAGTCTGGTGCCGGAAACAACTGGGCGAAGGGTCACTACACCGAGGGCGCGGAGTTGATCGATTCTGTTCTCGATGTGGTTCGCAAGGAGGCTGAGAATTGTGATTGCCTGCAAGGGTTTCAGGTCTGTCATTCTCTAGGAGGAGGTACTGGGTCTGGCATGGGTACTCTGTTGATTTCAAAGATCAGAGAGGAATACCCAGATCGGATGATGCTCACTTTCTCGGTCTTCCCCTCCCCGAAGGTCTCAGACACAGTTGTTGAGCCCTATAATGCAACCCTGTCTGTGCACCAGCTTGTTGAGAACGCAGATGAGTGTATGGTCCTTGATAATGAAGCTCTTTACGACATTTGCTTCCGCACACTCAAGCTCAGCACCCCAAGCTTTGGAGATTTGAACCATTTGATTTCTGCAACTATGTCTGGTGTAACATGCTGTTTGAGGTTCCCTGGTCAGTTGAACTCTGACCTTCGAAAGCTTGCTGTCAATCTCATCCCTTTCCCTCGTCTCCACTTCTTCATGGTCGGTTTTGCTCCTTTGACATCACGTGGTTCACAGCAGTATAGATCTCTCACAGTCCCTGAGCTCACACAGCAAATGTGGGATTCGAAGAACATGATGTGCGCAGCCGACCCTCGTCATGGTCGGTATCTGACCGCCTCTGCCATGTTCAGGGGTAAAATGAGCACTAAGGAGGTTGATGAACAGATCGATCAATGTCCAGAACAAGAACTCCTCTTACTTTGTGGAATGGATTCCCCACAATGTCAAGTCAACTGTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

355

Amino Acids

39.3

Weight (kDa)

5.02

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 3 - 211 6.4e-65 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 261 - 337 4e-22 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012720)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62690 AT5G62700
fragaria_vesca FvH4_6g12290
malus_domestica MD04G1143700.v1.1 MD12G1158100.v1.1
prunus_persica Prupe.6G268300_v2.0.a1
pyrus_communis pycom04g13060 pycom05g25220 pycom06g07900 pycom07g24790 pycom11g07310 pycom12g15020
rosa_chinensis RchiOBHm_Chr3g0464121
rosa_laevigata RLG00000024721
rosa_multiflora Rmu_sc0010401.1_g000003
rosa_roxburghii Rroxscaffold_6G00415980
rosa_rugosa Rorug03G0070700
rosa_wichuraiana Rw3G010800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 243
Acc16I TGCGCA 1 cut(s) 904
AccB1I GGYRCC 3 cut(s) 50, 95, 287
AccB7I CCANNNNNTGG 2 cut(s) 60, 665
AccII CGCG 2 cut(s) 119, 329
AciI CCGC 6 cut(s) 36, 119, 168, 329, 637, 936
AclWI GGATC 1 cut(s) 146
AcoI YGGCCR 2 cut(s) 28, 277
AcuI CTGAAG 1 cut(s) 238
AcvI CACGTG 1 cut(s) 827
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 4 cut(s) 107, 176, 427, 445
AfiI CCNNNNNNNGG 5 cut(s) 60, 168, 665, 920, 951
AgsI TTSAA 3 cut(s) 459, 676, 739
AjnI CCWGG 2 cut(s) 208, 727
AloI GAACNNNNNNTCC 2 cut(s) 996, 1028
AluBI AGCT 8 cut(s) 124, 130, 574, 617, 649, 663, 757, 865
AluI AGCT 8 cut(s) 124, 130, 574, 617, 649, 663, 757, 865
Alw21I GWGCWC 6 cut(s) 84, 126, 195, 570, 867, 968
Alw26I GTCTC 3 cut(s) 108, 529, 791
Alw44I GTGCAC 1 cut(s) 566
AlwI GGATC 1 cut(s) 146
AlwNI CAGNNNCTG 1 cut(s) 860
AoxI GGCC 3 cut(s) 28, 257, 277
ApaLI GTGCAC 1 cut(s) 566
ApeKI GCWGC 1 cut(s) 905
Asp700I GAANNNNTTC 1 cut(s) 1033
AspLEI GCGC 4 cut(s) 53, 77, 329, 905
AspS9I GGNCC 4 cut(s) 199, 213, 236, 601
AsuII TTCGAA 2 cut(s) 751, 887
AvaII GGWCC 4 cut(s) 199, 213, 236, 601
BaeGI GKGCMC 1 cut(s) 570
BalI TGGCCA 1 cut(s) 30
BanI GGYRCC 3 cut(s) 50, 95, 287
BanII GRGCYC 3 cut(s) 126, 546, 867
BarI GAAGNNNNNNTAC 2 cut(s) 299, 331
BbrPI CACGTG 1 cut(s) 827
BbsI GAAGAC 1 cut(s) 499
Bbv12I GWGCWC 6 cut(s) 84, 126, 195, 570, 867, 968
BbvI GCAGC 1 cut(s) 917
BceAI ACGGC 2 cut(s) 100, 109
BcgI CGANNNNNNTGC 4 cut(s) 28, 62, 172, 206
BciT130I CCWGG 2 cut(s) 210, 729
BcoDI GTCTC 3 cut(s) 108, 529, 791
BfaI CTAG 1 cut(s) 416
BfoI RGCGCY 1 cut(s) 54
BglI GCCNNNNNGGC 1 cut(s) 36
BglII AGATCT 3 cut(s) 230, 249, 845
BisI GCNGC 2 cut(s) 37, 906
BlpI GCTNAGC 1 cut(s) 650
BlsI GCNGC 2 cut(s) 38, 907
Bme1390I CCNGG 2 cut(s) 210, 729
Bme18I GGWCC 4 cut(s) 199, 213, 236, 601
BmgT120I GGNCC 4 cut(s) 199, 213, 236, 601
BmiI GGNNCC 6 cut(s) 52, 97, 214, 238, 289, 725
BmrFI CCNGG 2 cut(s) 210, 729
BmrI ACTGGG 2 cut(s) 310, 438
BmsI GCATC 2 cut(s) 96, 480
BmuI ACTGGG 2 cut(s) 310, 438
BoxI GACNNNNGTC 1 cut(s) 915
BpiI GAAGAC 1 cut(s) 499
BpmI CTGGAG 1 cut(s) 110
Bpu10I CCTNAGC 1 cut(s) 861
Bpu1102I GCTNAGC 1 cut(s) 650
Bpu14I TTCGAA 2 cut(s) 751, 887
BpuEI CTTGAG 3 cut(s) 152, 224, 629
Bsa29I ATCGAT 2 cut(s) 339, 990
BsaAI YACGTR 2 cut(s) 178, 827
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 529
BsaJI CCNNGG 6 cut(s) 22, 182, 209, 216, 321, 727
BsaWI WCCGGW 1 cut(s) 98
BsaXI ACNNNNNCTCC 2 cut(s) 996, 1026
Bsc4I CCNNNNNNNGG 5 cut(s) 60, 168, 665, 920, 951
Bse1I ACTGG 5 cut(s) 31, 162, 280, 305, 433
BseBI CCWGG 2 cut(s) 210, 729
BseCI ATCGAT 2 cut(s) 339, 990
BseDI CCNNGG 6 cut(s) 22, 182, 209, 216, 321, 727
BseGI GGATG 2 cut(s) 492, 771
BseLI CCNNNNNNNGG 5 cut(s) 60, 168, 665, 920, 951
BseMII CTCAG 4 cut(s) 363, 540, 664, 852
BseNI ACTGG 5 cut(s) 31, 162, 280, 305, 433
BseRI GAGGAG 2 cut(s) 435, 1004
BseSI GKGCMC 1 cut(s) 570
BseXI GCAGC 1 cut(s) 917
Bsh1236I CGCG 2 cut(s) 119, 329
BshFI GGCC 3 cut(s) 30, 259, 279
BshNI GGYRCC 3 cut(s) 50, 95, 287
BshVI ATCGAT 2 cut(s) 339, 990
BsiHKAI GWGCWC 6 cut(s) 84, 126, 195, 570, 867, 968
BsiSI CCGG 2 cut(s) 99, 291
BslFI GGGAC 2 cut(s) 226, 842
BslI CCNNNNNNNGG 5 cut(s) 60, 168, 665, 920, 951
BsmAI GTCTC 3 cut(s) 108, 529, 791
BsmBI CGTCTC 2 cut(s) 108, 791
BsmFI GGGAC 2 cut(s) 226, 842
BsnI GGCC 3 cut(s) 30, 259, 279
Bso31I GGTCTC 1 cut(s) 529
Bsp119I TTCGAA 2 cut(s) 751, 887
Bsp1286I GDGCHC 7 cut(s) 84, 126, 195, 546, 570, 867, 968
Bsp1720I GCTNAGC 1 cut(s) 650
Bsp19I CCATGG 1 cut(s) 216
BspACI CCGC 6 cut(s) 36, 119, 168, 329, 637, 936
BspANI GGCC 3 cut(s) 30, 259, 279
BspCNI CTCAG 4 cut(s) 364, 539, 663, 853
BspDI ATCGAT 2 cut(s) 339, 990
BspFNI CGCG 2 cut(s) 119, 329
BspLI GGNNCC 6 cut(s) 52, 97, 214, 238, 289, 725
BspPI GGATC 1 cut(s) 146
BspT104I TTCGAA 2 cut(s) 751, 887
BspT107I GGYRCC 3 cut(s) 50, 95, 287
BspTNI GGTCTC 1 cut(s) 529
BsrI ACTGG 5 cut(s) 31, 162, 280, 305, 433
BssECI CCNNGG 6 cut(s) 22, 182, 209, 216, 321, 727
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 2 cut(s) 22, 216
Bst2UI CCWGG 2 cut(s) 210, 729
Bst4CI ACNGT 4 cut(s) 245, 535, 856, 1058
BstACI GRCGYC 1 cut(s) 51
BstBAI YACGTR 2 cut(s) 178, 827
BstBI TTCGAA 2 cut(s) 751, 887
BstC8I GCNNGC 2 cut(s) 389, 759
BstDEI CTNAG 5 cut(s) 372, 526, 650, 861, 969
BstDSI CCRYGG 1 cut(s) 216
BstF5I GGATG 2 cut(s) 492, 771
BstFNI CGCG 2 cut(s) 119, 329
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 4 cut(s) 53, 77, 329, 905
BstMAI GTCTC 3 cut(s) 108, 529, 791
BstMWI GCNNNNNNNGC 4 cut(s) 36, 93, 660, 871
BstNI CCWGG 2 cut(s) 210, 729
BstNSI RCATGY 1 cut(s) 714
BstPAI GACNNNNGTC 1 cut(s) 915
BstSCI CCNGG 2 cut(s) 208, 727
BstSLI GKGCMC 1 cut(s) 570
BstUI CGCG 2 cut(s) 119, 329
BstV1I GCAGC 1 cut(s) 917
BstV2I GAAGAC 1 cut(s) 499
BstX2I RGATCY 3 cut(s) 230, 249, 845
BstYI RGATCY 3 cut(s) 230, 249, 845
Bsu15I ATCGAT 2 cut(s) 339, 990
BsuRI GGCC 3 cut(s) 30, 259, 279
BsuTUI ATCGAT 2 cut(s) 339, 990
BtgI CCRYGG 1 cut(s) 216
BtsCI GGATG 2 cut(s) 492, 771
BtsIMutI CAGTG 1 cut(s) 38
Cac8I GCNNGC 2 cut(s) 389, 759
CaiI CAGNNNCTG 1 cut(s) 860
CfoI GCGC 4 cut(s) 53, 77, 329, 905
Cfr13I GGNCC 4 cut(s) 199, 213, 236, 601
ClaI ATCGAT 2 cut(s) 339, 990
CseI GACGC 2 cut(s) 125, 214
Csp6I GTAC 4 cut(s) 106, 175, 426, 444
CviAII CATG 8 cut(s) 196, 217, 439, 711, 799, 895, 920, 946
CviQI GTAC 4 cut(s) 106, 175, 426, 444
DdeI CTNAG 5 cut(s) 372, 526, 650, 861, 969
DinI GGCGCC 1 cut(s) 52
DrdI GACNNNNNNGTC 1 cut(s) 243
DseDI GACNNNNNNGTC 1 cut(s) 243
EaeI YGGCCR 2 cut(s) 28, 277
Ecl136II GAGCTC 2 cut(s) 124, 865
Eco130I CCWWGG 2 cut(s) 22, 216
Eco147I AGGCCT 1 cut(s) 259
Eco24I GRGCYC 3 cut(s) 126, 546, 867
Eco31I GGTCTC 1 cut(s) 529
Eco47I GGWCC 4 cut(s) 199, 213, 236, 601
Eco53kI GAGCTC 2 cut(s) 124, 865
Eco57I CTGAAG 1 cut(s) 238
Eco72I CACGTG 1 cut(s) 827
EcoICRI GAGCTC 2 cut(s) 124, 865
EcoRII CCWGG 2 cut(s) 208, 727
EcoT14I CCWWGG 2 cut(s) 22, 216
EcoT38I GRGCYC 3 cut(s) 126, 546, 867
EgeI GGCGCC 1 cut(s) 52
EheI GGCGCC 1 cut(s) 52
ErhI CCWWGG 2 cut(s) 22, 216
Esp3I CGTCTC 2 cut(s) 108, 791
FaeI CATG 8 cut(s) 199, 220, 442, 714, 802, 898, 923, 949
FaqI GGGAC 2 cut(s) 226, 842
FatI CATG 8 cut(s) 195, 216, 438, 710, 798, 894, 919, 945
FauI CCCGC 1 cut(s) 161
Fnu4HI GCNGC 2 cut(s) 37, 906
FokI GGATG 2 cut(s) 499, 758
FriOI GRGCYC 3 cut(s) 126, 546, 867
Fsp4HI GCNGC 2 cut(s) 37, 906
FspBI CTAG 1 cut(s) 416
FspI TGCGCA 1 cut(s) 904
GlaI GCGC 4 cut(s) 52, 76, 328, 904
GluI GCNGC 2 cut(s) 37, 906
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 3 cut(s) 30, 259, 279
HapII CCGG 2 cut(s) 99, 291
HgaI GACGC 2 cut(s) 125, 214
HhaI GCGC 4 cut(s) 53, 77, 329, 905
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 8 cut(s) 199, 220, 442, 714, 802, 898, 923, 949
Hin6I GCGC 4 cut(s) 51, 75, 327, 903
HinP1I GCGC 4 cut(s) 51, 75, 327, 903
HincII GTYRAC 1 cut(s) 1054
HindII GTYRAC 1 cut(s) 1054
HindIII AAGCTT 2 cut(s) 661, 755
HinfI GANTC 4 cut(s) 10, 341, 884, 1034
HpaII CCGG 2 cut(s) 99, 291
Hpy166II GTNNAC 4 cut(s) 148, 568, 833, 1054
Hpy188I TCNGA 7 cut(s) 230, 249, 467, 486, 529, 745, 933
Hpy188III TCNNGA 3 cut(s) 234, 350, 1001
Hpy8I GTNNAC 4 cut(s) 148, 568, 833, 1054
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 4 cut(s) 96, 301, 514, 758
HpyCH4III ACNGT 4 cut(s) 245, 535, 856, 1058
HpyCH4IV ACGT 2 cut(s) 177, 826
HpyCH4V TGCA 4 cut(s) 391, 554, 568, 692
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 93, 660, 871
HpyF3I CTNAG 5 cut(s) 372, 526, 650, 861, 969
HpySE526I ACGT 2 cut(s) 177, 826
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 8 cut(s) 199, 220, 442, 714, 802, 898, 923, 949
HspAI GCGC 4 cut(s) 51, 75, 327, 903
KasI GGCGCC 1 cut(s) 50
LmnI GCTCC 3 cut(s) 121, 129, 817
Lsp1109I GCAGC 1 cut(s) 917
LweI GCATC 2 cut(s) 96, 480
MaeI CTAG 1 cut(s) 416
MaeII ACGT 2 cut(s) 177, 826
MaeIII GTNAC 2 cut(s) 311, 706
MboII GAAGA 5 cut(s) 5, 244, 499, 787, 901
MflI RGATCY 3 cut(s) 230, 249, 845
MhlI GDGCHC 7 cut(s) 84, 126, 195, 546, 570, 867, 968
MlsI TGGCCA 1 cut(s) 30
MluCI AATT 1 cut(s) 376
MluNI TGGCCA 1 cut(s) 30
Mly113I GGCGCC 1 cut(s) 51
Mox20I TGGCCA 1 cut(s) 30
MroXI GAANNNNTTC 1 cut(s) 1033
MscI TGGCCA 1 cut(s) 30
Msp20I TGGCCA 1 cut(s) 30
MspI CCGG 2 cut(s) 99, 291
MspR9I CCNGG 2 cut(s) 210, 729
MvaI CCWGG 2 cut(s) 210, 729
MvnI CGCG 2 cut(s) 119, 329
MwoI GCNNNNNNNGC 4 cut(s) 36, 93, 660, 871
NarI GGCGCC 1 cut(s) 51
NcoI CCATGG 1 cut(s) 216
NlaIII CATG 8 cut(s) 199, 220, 442, 714, 802, 898, 923, 949
NlaIV GGNNCC 6 cut(s) 52, 97, 214, 238, 289, 725
NmeAIII GCCGAG 2 cut(s) 103, 207
NmuCI GTSAC 1 cut(s) 311
NsbI TGCGCA 1 cut(s) 904
NspI RCATGY 1 cut(s) 714
NspV TTCGAA 2 cut(s) 751, 887
PceI AGGCCT 1 cut(s) 259
PdmI GAANNNNTTC 1 cut(s) 1033
PfeI GAWTC 4 cut(s) 10, 341, 884, 1034
PflFI GACNNNGTC 1 cut(s) 224
PflMI CCANNNNNTGG 2 cut(s) 60, 665
PkrI GCNGC 2 cut(s) 38, 907
PluTI GGCGCC 1 cut(s) 54
PmaCI CACGTG 1 cut(s) 827
PmlI CACGTG 1 cut(s) 827
Ppu21I YACGTR 2 cut(s) 178, 827
PshAI GACNNNNGTC 1 cut(s) 915
Psp124BI GAGCTC 2 cut(s) 126, 867
Psp6I CCWGG 2 cut(s) 208, 727
PspCI CACGTG 1 cut(s) 827
PspGI CCWGG 2 cut(s) 208, 727
PspN4I GGNNCC 6 cut(s) 52, 97, 214, 238, 289, 725
PspPI GGNCC 4 cut(s) 199, 213, 236, 601
PsrI GAACNNNNNNTAC 2 cut(s) 1002, 1034
PstNI CAGNNNCTG 1 cut(s) 860
PsuI RGATCY 3 cut(s) 230, 249, 845
PsyI GACNNNGTC 1 cut(s) 224
RsaI GTAC 4 cut(s) 107, 176, 427, 445
RsaNI GTAC 4 cut(s) 106, 175, 426, 444
SacI GAGCTC 2 cut(s) 126, 867
SatI GCNGC 2 cut(s) 37, 906
Sau96I GGNCC 4 cut(s) 199, 213, 236, 601
ScrFI CCNGG 2 cut(s) 210, 729
SduI GDGCHC 7 cut(s) 84, 126, 195, 546, 570, 867, 968
SfaNI GCATC 2 cut(s) 96, 480
SfoI GGCGCC 1 cut(s) 52
SfuI TTCGAA 2 cut(s) 751, 887
SinI GGWCC 4 cut(s) 199, 213, 236, 601
SmlI CTYRAG 3 cut(s) 131, 203, 644
SmoI CTYRAG 3 cut(s) 131, 203, 644
Sse9I AATT 1 cut(s) 376
SseBI AGGCCT 1 cut(s) 259
SsiI CCGC 6 cut(s) 36, 119, 168, 329, 637, 936
SspDI GGCGCC 1 cut(s) 50
SspMI CTAG 1 cut(s) 416
SstI GAGCTC 2 cut(s) 126, 867
StuI AGGCCT 1 cut(s) 259
StyD4I CCNGG 2 cut(s) 208, 727
StyI CCWWGG 2 cut(s) 22, 216
TaaI ACNGT 4 cut(s) 245, 535, 856, 1058
TaiI ACGT 2 cut(s) 180, 829
TaqI TCGA 6 cut(s) 90, 339, 351, 751, 887, 990
TaqII GACCGA 1 cut(s) 493
TasI AATT 1 cut(s) 376
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 4 cut(s) 10, 341, 884, 1034
TscAI CASTG 1 cut(s) 38
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 1 cut(s) 905
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 4 cut(s) 5, 627, 787, 996
TspRI CASTG 1 cut(s) 38
Tth111I GACNNNGTC 1 cut(s) 224
Van91I CCANNNNNTGG 2 cut(s) 60, 665
VneI GTGCAC 1 cut(s) 566
VpaK11BI GGWCC 4 cut(s) 199, 213, 236, 601
XceI RCATGY 1 cut(s) 714
XmnI GAANNNNTTC 1 cut(s) 1033
XspI CTAG 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.