pycom12g19080

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0008214
Physical Location & Seq
Forward (+)
21038723 .. 21042705
3983 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 798 bp
ATGGATATGGAAAAAAAGGCTGTTGAATTATTTGATGAGAGACCTGATCATATCGTGTTTATGGCAGTTCCGAGTGCTTGCGGTCACACCGGCCTTGTTGACGACGGCTTGAGATATTTCGAATCAGCAGTTATCCCAAACTCGGCGTTATATATGAAGCTGAAAGTGAGAAGGCAACAGAAAAAGCAAAAAGTGAAAGGCTTAACACTGCCCTTTTCCTACTCTCTGAAATTAAGCATCAACCCCCAAAGGGCACCCAGAATGCCTCGGAAGCGCAGAGACAATCCCGACCCGAATTTCGACACCCGATCCCCCGGCAAGAAACGGGCGGCTCCTCTGGAGGAAAATGGAGGAGAGAAAGTGCCAATTGTAACTCACAAGAAATTTACCAAGGGAATGCCGGTTGAGGTTTGCAGTGATGAAGATGGTTTCCATGGTGCTTGGTTTGCTGCAACCATAGTGGAAGCGGTGGGGAATGACAAGTTCCTCATTGAGTACCAGAGCCTAAGGACCGAAGATAATTCAGCGTTTCTGAGAGAAGAGATTGATACCCTGCACATACGACCTAATCCGCCGGAAATTGCTGTTGAGCATTTCAGTTTGCTTCAAGAGGATGATGCTTTGTACAATGATGGCTGGTGGGTTGGTGTGATCTCGAAAGTTCTTAGCGGCTCAAGGTACATAGTCTACTTCAGGAGCACCTATGAAGAAATCGAGTTTCAGCAATCTGAGTTGAGGCTTCATCAGGACTGGATTGGTGGAAAATGGGTTATGTCTTCTGGGGCTTTAAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

266

Amino Acids

30.3

Weight (kDa)

6.67

Isoelectric Point (pI)

57.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 132 - 192 3.1e-20 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 253
AccI GTMKAC 1 cut(s) 687
AciI CCGC 5 cut(s) 81, 329, 467, 572, 669
AclWI GGATC 1 cut(s) 303
AcsI RAATTY 2 cut(s) 295, 383
AcuI CTGAAG 1 cut(s) 676
AfaI GTAC 3 cut(s) 497, 626, 680
AfiI CCNNNNNNNGG 2 cut(s) 142, 250
AgsI TTSAA 2 cut(s) 26, 608
AluBI AGCT 1 cut(s) 160
AluI AGCT 1 cut(s) 160
Alw21I GWGCWC 1 cut(s) 701
Alw26I GTCTC 2 cut(s) 34, 273
AlwI GGATC 1 cut(s) 303
AoxI GGCC 1 cut(s) 91
ApeKI GCWGC 1 cut(s) 449
ApoI RAATTY 2 cut(s) 295, 383
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 510
AsuC2I CCSGG 1 cut(s) 315
AsuII TTCGAA 1 cut(s) 120
AvaII GGWCC 1 cut(s) 510
AxyI CCTNAGG 1 cut(s) 506
BaeGI GKGCMC 1 cut(s) 256
BanI GGYRCC 1 cut(s) 253
BbsI GAAGAC 1 cut(s) 768
Bbv12I GWGCWC 1 cut(s) 701
BbvI GCAGC 1 cut(s) 436
BccI CCATC 2 cut(s) 419, 626
BceAI ACGGC 1 cut(s) 121
BclI TGATCA 1 cut(s) 46
BcnI CCSGG 1 cut(s) 315
BcoDI GTCTC 2 cut(s) 34, 273
BisI GCNGC 3 cut(s) 330, 450, 670
BlsI GCNGC 3 cut(s) 331, 451, 671
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 510
BmgT120I GGNCC 1 cut(s) 510
BmiI GGNNCC 2 cut(s) 255, 333
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 2 cut(s) 246, 607
BpiI GAAGAC 1 cut(s) 768
BpmI CTGGAG 1 cut(s) 359
Bpu14I TTCGAA 1 cut(s) 120
BpuEI CTTGAG 2 cut(s) 130, 658
BpuMI CCSGG 1 cut(s) 315
BsaI GGTCTC 1 cut(s) 34
BsaJI CCNNGG 4 cut(s) 266, 313, 390, 433
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 250
Bse118I RCCGGY 2 cut(s) 89, 400
Bse1I ACTGG 1 cut(s) 755
Bse21I CCTNAGG 1 cut(s) 506
BseDI CCNNGG 4 cut(s) 266, 313, 390, 433
BseGI GGATG 1 cut(s) 619
BseLI CCNNNNNNNGG 2 cut(s) 142, 250
BseMII CTCAG 2 cut(s) 524, 720
BseNI ACTGG 1 cut(s) 755
BseRI GAGGAG 2 cut(s) 324, 366
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 1 cut(s) 436
BsgI GTGCAG 1 cut(s) 539
BshFI GGCC 1 cut(s) 93
BshNI GGYRCC 1 cut(s) 253
BsiHKAI GWGCWC 1 cut(s) 701
BsiSI CCGG 4 cut(s) 90, 315, 401, 575
BslI CCNNNNNNNGG 2 cut(s) 142, 250
BsmAI GTCTC 2 cut(s) 34, 273
BsmI GAATGC 2 cut(s) 267, 402
BsnI GGCC 1 cut(s) 93
Bso31I GGTCTC 1 cut(s) 34
Bsp119I TTCGAA 1 cut(s) 120
Bsp1286I GDGCHC 2 cut(s) 256, 701
Bsp1407I TGTACA 1 cut(s) 624
Bsp143I GATC 3 cut(s) 46, 308, 651
Bsp19I CCATGG 1 cut(s) 433
BspACI CCGC 5 cut(s) 81, 329, 467, 572, 669
BspANI GGCC 1 cut(s) 93
BspCNI CTCAG 2 cut(s) 525, 721
BspLI GGNNCC 2 cut(s) 255, 333
BspPI GGATC 1 cut(s) 303
BspT104I TTCGAA 1 cut(s) 120
BspT107I GGYRCC 1 cut(s) 253
BspTNI GGTCTC 1 cut(s) 34
BsrFI RCCGGY 2 cut(s) 89, 400
BsrGI TGTACA 1 cut(s) 624
BsrI ACTGG 1 cut(s) 755
BssAI RCCGGY 2 cut(s) 89, 400
BssECI CCNNGG 4 cut(s) 266, 313, 390, 433
BssMI GATC 3 cut(s) 46, 308, 651
BssT1I CCWWGG 2 cut(s) 390, 433
Bst6I CTCTTC 1 cut(s) 534
BstAUI TGTACA 1 cut(s) 624
BstBI TTCGAA 1 cut(s) 120
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 4 cut(s) 506, 533, 665, 729
BstDSI CCRYGG 1 cut(s) 433
BstF5I GGATG 1 cut(s) 619
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 3 cut(s) 49, 311, 654
BstMAI GTCTC 2 cut(s) 34, 273
BstMBI GATC 3 cut(s) 46, 308, 651
BstMWI GCNNNNNNNGC 2 cut(s) 271, 446
BstSCI CCNGG 1 cut(s) 313
BstSLI GKGCMC 1 cut(s) 256
BstV1I GCAGC 1 cut(s) 436
BstV2I GAAGAC 1 cut(s) 768
Bsu36I CCTNAGG 1 cut(s) 506
BsuRI GGCC 1 cut(s) 93
BtgI CCRYGG 1 cut(s) 433
BtsCI GGATG 1 cut(s) 619
BtsI GCAGTG 2 cut(s) 206, 421
BtsIMutI CAGTG 2 cut(s) 206, 421
Cac8I GCNNGC 1 cut(s) 79
CfoI GCGC 1 cut(s) 276
Cfr10I RCCGGY 2 cut(s) 89, 400
Cfr13I GGNCC 1 cut(s) 510
Csp6I GTAC 3 cut(s) 496, 625, 679
CspCI CAANNNNNGTGG 2 cut(s) 441, 476
CviAII CATG 1 cut(s) 434
CviQI GTAC 3 cut(s) 496, 625, 679
DdeI CTNAG 4 cut(s) 506, 533, 665, 729
DpnI GATC 3 cut(s) 48, 310, 653
DpnII GATC 3 cut(s) 46, 308, 651
DraI TTTAAA 1 cut(s) 789
Eam1104I CTCTTC 1 cut(s) 534
EarI CTCTTC 1 cut(s) 534
EciI GGCGGA 1 cut(s) 561
Eco130I CCWWGG 2 cut(s) 390, 433
Eco31I GGTCTC 1 cut(s) 34
Eco47I GGWCC 1 cut(s) 510
Eco57I CTGAAG 1 cut(s) 676
Eco81I CCTNAGG 1 cut(s) 506
EcoT14I CCWWGG 2 cut(s) 390, 433
ErhI CCWWGG 2 cut(s) 390, 433
FaeI CATG 1 cut(s) 437
FatI CATG 1 cut(s) 433
FbaI TGATCA 1 cut(s) 46
FblI GTMKAC 1 cut(s) 687
Fnu4HI GCNGC 3 cut(s) 330, 450, 670
FokI GGATG 1 cut(s) 626
Fsp4HI GCNGC 3 cut(s) 330, 450, 670
GlaI GCGC 1 cut(s) 275
GluI GCNGC 3 cut(s) 330, 450, 670
GsuI CTGGAG 1 cut(s) 359
HaeIII GGCC 1 cut(s) 93
HapII CCGG 4 cut(s) 90, 315, 401, 575
HhaI GCGC 1 cut(s) 276
Hin1II CATG 1 cut(s) 437
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 1 cut(s) 122
HpaII CCGG 4 cut(s) 90, 315, 401, 575
Hpy166II GTNNAC 2 cut(s) 100, 688
Hpy188I TCNGA 5 cut(s) 72, 228, 270, 534, 730
Hpy188III TCNNGA 6 cut(s) 287, 338, 608, 655, 694, 746
Hpy8I GTNNAC 2 cut(s) 100, 688
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 1 cut(s) 165
HpyCH4V TGCA 3 cut(s) 414, 452, 556
HpyF10VI GCNNNNNNNGC 2 cut(s) 271, 446
HpyF3I CTNAG 4 cut(s) 506, 533, 665, 729
Hsp92II CATG 1 cut(s) 437
HspAI GCGC 1 cut(s) 274
Ksp22I TGATCA 1 cut(s) 46
Kzo9I GATC 3 cut(s) 46, 308, 651
LmnI GCTCC 2 cut(s) 337, 696
Lsp1109I GCAGC 1 cut(s) 436
LweI GCATC 2 cut(s) 246, 607
MaeIII GTNAC 2 cut(s) 83, 370
MalI GATC 3 cut(s) 48, 310, 653
MboI GATC 3 cut(s) 46, 308, 651
MboII GAAGA 5 cut(s) 434, 527, 551, 719, 768
MfeI CAATTG 1 cut(s) 366
MhlI GDGCHC 2 cut(s) 256, 701
MluCI AATT 7 cut(s) 26, 230, 295, 366, 383, 520, 579
MnlI CCTC 8 cut(s) 276, 334, 344, 345, 400, 497, 604, 729
MseI TTAA 3 cut(s) 203, 233, 788
MspI CCGG 4 cut(s) 90, 315, 401, 575
MspR9I CCNGG 1 cut(s) 315
MunI CAATTG 1 cut(s) 366
Mva1269I GAATGC 2 cut(s) 267, 402
MwoI GCNNNNNNNGC 2 cut(s) 271, 446
NciI CCSGG 1 cut(s) 315
NcoI CCATGG 1 cut(s) 433
NdeII GATC 3 cut(s) 46, 308, 651
NlaIII CATG 1 cut(s) 437
NlaIV GGNNCC 2 cut(s) 255, 333
NmeAIII GCCGAG 1 cut(s) 122
NmuCI GTSAC 1 cut(s) 83
NspV TTCGAA 1 cut(s) 120
PctI GAATGC 2 cut(s) 267, 402
PfeI GAWTC 1 cut(s) 122
PkrI GCNGC 3 cut(s) 331, 451, 671
PspN4I GGNNCC 2 cut(s) 255, 333
PspPI GGNCC 1 cut(s) 510
RsaI GTAC 3 cut(s) 497, 626, 680
RsaNI GTAC 3 cut(s) 496, 625, 679
SaqAI TTAA 3 cut(s) 203, 233, 788
SatI GCNGC 3 cut(s) 330, 450, 670
Sau3AI GATC 3 cut(s) 46, 308, 651
Sau96I GGNCC 1 cut(s) 510
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 2 cut(s) 256, 701
SetI ASST 6 cut(s) 46, 162, 411, 568, 680, 704
SfaNI GCATC 2 cut(s) 246, 607
SfuI TTCGAA 1 cut(s) 120
SinI GGWCC 1 cut(s) 510
SmlI CTYRAG 2 cut(s) 109, 673
SmoI CTYRAG 2 cut(s) 109, 673
Sse9I AATT 7 cut(s) 26, 230, 295, 366, 383, 520, 579
SsiI CCGC 5 cut(s) 81, 329, 467, 572, 669
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 2 cut(s) 390, 433
TaqI TCGA 4 cut(s) 120, 300, 656, 714
TaqII GACCGA 1 cut(s) 527
TasI AATT 7 cut(s) 26, 230, 295, 366, 383, 520, 579
TatI WGTACW 1 cut(s) 624
TauI GCSGC 2 cut(s) 332, 672
TfiI GAWTC 1 cut(s) 122
Tru1I TTAA 3 cut(s) 203, 233, 788
Tru9I TTAA 3 cut(s) 203, 233, 788
TscAI CASTG 2 cut(s) 213, 421
TseFI GTSAC 1 cut(s) 83
TseI GCWGC 1 cut(s) 449
Tsp45I GTSAC 1 cut(s) 83
TspDTI ATGAA 4 cut(s) 170, 435, 720, 731
TspRI CASTG 2 cut(s) 213, 421
VpaK11BI GGWCC 1 cut(s) 510
XapI RAATTY 2 cut(s) 295, 383
XmiI GTMKAC 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.