Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0006224

Physical Location & Seq

Genomic Coordinates Reverse (-)

21065125 .. 21070615

Gene Structure 5491 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1452 bp

ATGAGGATTGTTTTAGGAAGACTTCTGCAGAGGATGCCACTGAAACAGGCGATTGGACGCCGCGCATGTTCAACGAAGAAGATTGTTGATGTTGGACAGCCAACTGCTGCATCTCATCCACAGTTGTTGGTGGAAGGAGAGATTACACCCGGGATAACTTCGGAGGAATACGAATTGAGAAGAAAAAAGTTGTTGGATGCGCTCCCAGAGAAGGGTTTGGCCATTTTTGCAGCTGCCCCTGTAAAGATGATGACCGATGTTGTGCCTTATACCTATCGTCAAGATGCTGATTACTTGTATATTACTGGCTGCCAACAGCCTGGTGGCGTGGCAGTTTTGGGTCGTGAATGCGGTTTTTGCATGTTCATGCCAGAAGCAACGACTCATGACGTTATTTGGCAAGGGGAAGTTGCGGGAGTTCGTGCAGCACTGGAAGTATTCAAGGCTGATAGAGCATACCCAATGAACAGATTACGGCAGATCCTGCCAGATATTATGAAGGGATCGTCCGAAATGTTCCACAATGTGCAGACGGCTGTACCAACGTATATGGAATTGGATCCATTTCAGAAAGCAGCTTACTCTGGAAAAGTGAAAGATTCTTCGGTTTTCACCCATGAGTTACGGTTAATAAAGTCACCGGCAGAGCTTAAGTTAATGAGAGAGTCAGCATCAATTGCTTGCCAAGCTCTACTGCAGACAATGTTTCACTCAAAGACACACCCTTATGAGGGTAGGCTAGCAGCTAAGATTGAATATGAATGCAAAATGAGGGGTGCCCAGAGAATGGCATTCAATCCTGTGGTTGGTGGGGGGCCCAATGCTAGTGTCATACATTATGCTCGGAATGATCAGAAAATAAAAGATGGGGAACTTGTCTTGATGGATGTTGGTTGCGAGCTTCATGGTTATGTCAGTGATCTTACTCGCACCTGGCCACCTTATGGCAGTTTCTCTTCAGCTCAGGAAGAGCTCTATGATCTTATTCTGCAAACAAACAAGACATGTATGGAGCTTTGCAAACCCGGTGCTAGCATTCGAGAAATACACAACTTTTCAGTTGAAATGCTTCACAAAGGACTCAAGGAGATTGGAATTCTGAAAGATTCTAGAAGCAGTTCCTACCATCAGCTGAATCCAACTTCCATAGGCCACTATCTGGGAATGGATGTCCATGATTGTTCTACGGTTGGTTATGACCGTCCGTTGAAGCCAGGCGTTGTCATAACAATTGAACCAGGAATCTACATCCCATTGTCTTCTAACTGTCCTGAAAGGTACCGAGGCATCGGGATAAGGATTGAGGATGAGGTCCTCATTACAGACACGGGTTATGAGGTTCTGACTGGGTCAATGCCGAAAGAAGTTAAACATATTGAATCCTTGCTAAACAACTTTCCCCATGGAAGTGTAATGGAGAACCACATTGCCGAGGGAGCTTCATCCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003008 GO:0003014 GO:0003094 GO:0003674 GO:0003824 GO:0004177 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008235 GO:0008237 GO:0008238 GO:0010467 GO:0016485 GO:0016787 GO:0019538 GO:0030145 GO:0031647 GO:0032501 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0046983 GO:0050821 GO:0051604 GO:0065007 GO:0065008 GO:0070006 GO:0070011 GO:0071704 GO:0097205 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AMP_N Peptidase_M24

Protein Analysis

484

Amino Acids

53.66

Weight (kDa)

6.61

Isoelectric Point (pI)

46.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP_N	PF05195	57 - 171	4.6e-32	Aminopeptidase P, N-terminal domain
Peptidase_M24	PF00557	219 - 442	1.9e-54	Metallopeptidase family M24

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006224)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09300 AT1G09300
fragaria_vesca	FvH4_6g07800 FvH4_6g07800 FvH4_6g07800 FvH4_6g07800 FvH4_6g07800
malus_domestica	MD04G1191800.v1.1
prunus_persica	Prupe.6G310400_v2.0.a1 Prupe.6G310400_v2.0.a1 Prupe.6G310400_v2.0.a1 Prupe.6G310400_v2.0.a1 Prupe.6G310400_v2.0.a1
pyrus_communis	pycom12g19110 pycom12g19120
rosa_chinensis	RchiOBHm_Chr3g0457511
rosa_laevigata	RLG00000025217
rosa_multiflora	Rmu_sc0007404.1_g000015 Rmu_sc0038466.1_g000001
rosa_roxburghii	Rroxscaffold_6G00421810
rosa_rugosa	Rorug03G0020600 Rorug03G0020900
rosa_samantha	Rh3AG081400 Rh3BG083500 Rh3CG083700 Rh3DG085100
rosa_wichuraiana	Rw3G006980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1278
AccB1I	GGYRCC	2 cut(s)	776, 1278
AccB7I	CCANNNNNTGG	3 cut(s)	787, 944, 1159
AccII	CGCG	1 cut(s)	63
AciI	CCGC	3 cut(s)	61, 351, 413
AclWI	GGATC	4 cut(s)	475, 511, 554, 567
AcoI	YGGCCR	2 cut(s)	219, 935
AcsI	RAATTY	1 cut(s)	1095
AcuI	CTGAAG	1 cut(s)	942
AcyI	GRCGYC	1 cut(s)	58
AdeI	CACNNNGTG	1 cut(s)	526
AfaI	GTAC	2 cut(s)	540, 1280
AfiI	CCNNNNNNNGG	8 cut(s)	211, 212, 730, 731, 787, 806, 944, 1159
AflII	CTTAAG	1 cut(s)	650
AflIII	ACRYGT	1 cut(s)	1004
AgsI	TTSAA	8 cut(s)	72, 442, 755, 796, 1064, 1210, 1235, 1379
AjnI	CCWGG	4 cut(s)	319, 932, 1213, 1237
Alw21I	GWGCWC	1 cut(s)	975
AlwI	GGATC	4 cut(s)	475, 511, 554, 567
AlwNI	CAGNNNCTG	1 cut(s)	484
Ama87I	CYCGRG	1 cut(s)	149
AoxI	GGCC	4 cut(s)	219, 815, 935, 1150
ApaI	GGGCCC	1 cut(s)	819
ApeKI	GCWGC	7 cut(s)	107, 230, 233, 309, 425, 575, 743
ApoI	RAATTY	1 cut(s)	1095
Asp700I	GAANNNNTTC	2 cut(s)	1068, 1117
Asp718I	GGTACC	1 cut(s)	1278
AspLEI	GCGC	2 cut(s)	65, 202
AspS9I	GGNCC	3 cut(s)	815, 816, 1312
AsuC2I	CCSGG	3 cut(s)	150, 151, 1026
AsuHPI	GGTGA	2 cut(s)	604, 630
AsuNHI	GCTAGC	2 cut(s)	739, 1031
AvaI	CYCGRG	1 cut(s)	149
AvaII	GGWCC	1 cut(s)	1312
BaeGI	GKGCMC	2 cut(s)	781, 819
BaeI	ACNNNNGTAYC	2 cut(s)	522, 555
BalI	TGGCCA	2 cut(s)	221, 937
BamHI	GGATCC	1 cut(s)	559
BanI	GGYRCC	2 cut(s)	776, 1278
BanII	GRGCYC	2 cut(s)	819, 975
BbsI	GAAGAC	2 cut(s)	25, 1251
Bbv12I	GWGCWC	1 cut(s)	975
BbvI	GCAGC	7 cut(s)	94, 220, 242, 296, 437, 587, 755
BccI	CCATC	3 cut(s)	860, 877, 1134
BceAI	ACGGC	2 cut(s)	491, 549
BciT130I	CCWGG	4 cut(s)	321, 934, 1215, 1239
BclI	TGATCA	1 cut(s)	850
BcnI	CCSGG	3 cut(s)	150, 151, 1026
BfaI	CTAG	4 cut(s)	740, 825, 1032, 1110
BfmI	CTRYAG	2 cut(s)	26, 695
BfrI	CTTAAG	1 cut(s)	650
BisI	GCNGC	8 cut(s)	61, 108, 231, 234, 310, 426, 576, 744
BlsI	GCNGC	8 cut(s)	62, 109, 232, 235, 311, 427, 577, 745
Bme1390I	CCNGG	7 cut(s)	150, 151, 321, 934, 1026, 1215, 1239
Bme18I	GGWCC	1 cut(s)	1312
BmeT110I	CYCGRG	1 cut(s)	149
BmgT120I	GGNCC	3 cut(s)	815, 816, 1312
BmiI	GGNNCC	5 cut(s)	561, 778, 816, 817, 1280
BmrFI	CCNGG	7 cut(s)	150, 151, 321, 934, 1026, 1215, 1239
BmrI	ACTGGG	1 cut(s)	1356
BmsI	GCATC	6 cut(s)	24, 119, 187, 274, 680, 1296
BmtI	GCTAGC	2 cut(s)	743, 1035
BmuI	ACTGGG	1 cut(s)	1356
BpiI	GAAGAC	2 cut(s)	25, 1251
Bpu10I	CCTNAGC	1 cut(s)	963
BpuEI	CTTGAG	1 cut(s)	1067
BpuMI	CCSGG	3 cut(s)	150, 151, 1026
BsaHI	GRCGYC	1 cut(s)	58
BsaJI	CCNNGG	4 cut(s)	149, 1282, 1402, 1431
Bsc4I	CCNNNNNNNGG	8 cut(s)	211, 212, 730, 731, 787, 806, 944, 1159
Bse118I	RCCGGY	1 cut(s)	640
Bse1I	ACTGG	4 cut(s)	310, 435, 1351, 1446
Bse3DI	GCAATG	1 cut(s)	1425
BseBI	CCWGG	4 cut(s)	321, 934, 1215, 1239
BseDI	CCNNGG	4 cut(s)	149, 1282, 1402, 1431
BseGI	GGATG	8 cut(s)	39, 115, 202, 892, 1174, 1248, 1312, 1442
BseLI	CCNNNNNNNGG	8 cut(s)	211, 212, 730, 731, 787, 806, 944, 1159
BseMI	GCAATG	1 cut(s)	1425
BseMII	CTCAG	1 cut(s)	977
BseNI	ACTGG	4 cut(s)	310, 435, 1351, 1446
BseSI	GKGCMC	2 cut(s)	781, 819
BseXI	GCAGC	7 cut(s)	94, 220, 242, 296, 437, 587, 755
BsgI	GTGCAG	2 cut(s)	444, 548
Bsh1236I	CGCG	1 cut(s)	63
BshFI	GGCC	4 cut(s)	221, 817, 937, 1152
BshNI	GGYRCC	2 cut(s)	776, 1278
BsiHKAI	GWGCWC	1 cut(s)	975
BsiHKCI	CYCGRG	1 cut(s)	149
BsiSI	CCGG	3 cut(s)	150, 641, 1026
BslI	CCNNNNNNNGG	8 cut(s)	211, 212, 730, 731, 787, 806, 944, 1159
BsmI	GAATGC	4 cut(s)	353, 767, 791, 1035
BsnI	GGCC	4 cut(s)	221, 817, 937, 1152
BsoBI	CYCGRG	1 cut(s)	149
Bsp120I	GGGCCC	1 cut(s)	815
Bsp1286I	GDGCHC	3 cut(s)	781, 819, 975
Bsp143I	GATC	6 cut(s)	480, 503, 559, 850, 919, 979
Bsp19I	CCATGG	1 cut(s)	1402
BspACI	CCGC	3 cut(s)	61, 351, 413
BspANI	GGCC	4 cut(s)	221, 817, 937, 1152
BspCNI	CTCAG	1 cut(s)	976
BspFNI	CGCG	1 cut(s)	63
BspHI	TCATGA	1 cut(s)	385
BspLI	GGNNCC	5 cut(s)	561, 778, 816, 817, 1280
BspMAI	CTGCAG	2 cut(s)	30, 699
BspOI	GCTAGC	2 cut(s)	743, 1035
BspPI	GGATC	4 cut(s)	475, 511, 554, 567
BspQI	GCTCTTC	1 cut(s)	963
BspT107I	GGYRCC	2 cut(s)	776, 1278
BspTI	CTTAAG	1 cut(s)	650
BsrDI	GCAATG	1 cut(s)	1425
BsrFI	RCCGGY	1 cut(s)	640
BsrI	ACTGG	4 cut(s)	310, 435, 1351, 1446
BssAI	RCCGGY	1 cut(s)	640
BssECI	CCNNGG	4 cut(s)	149, 1282, 1402, 1431
BssMI	GATC	6 cut(s)	480, 503, 559, 850, 919, 979
BssNI	GRCGYC	1 cut(s)	58
BssT1I	CCWWGG	1 cut(s)	1402
Bst2UI	CCWGG	4 cut(s)	321, 934, 1215, 1239
Bst4CI	ACNGT	5 cut(s)	123, 627, 1189, 1202, 1268
Bst6I	CTCTTC	2 cut(s)	961, 963
BstACI	GRCGYC	1 cut(s)	58
BstAFI	CTTAAG	1 cut(s)	650
BstAPI	GCANNNNNTGC	3 cut(s)	34, 484, 677
BstC8I	GCNNGC	4 cut(s)	682, 741, 899, 1033
BstDEI	CTNAG	2 cut(s)	747, 963
BstDSI	CCRYGG	1 cut(s)	1402
BstF5I	GGATG	8 cut(s)	39, 115, 202, 892, 1174, 1248, 1312, 1442
BstFNI	CGCG	1 cut(s)	63
BstHHI	GCGC	2 cut(s)	65, 202
BstKTI	GATC	6 cut(s)	483, 506, 562, 853, 922, 982
BstMBI	GATC	6 cut(s)	480, 503, 559, 850, 919, 979
BstMWI	GCNNNNNNNGC	8 cut(s)	34, 227, 357, 452, 484, 677, 686, 1436
BstNI	CCWGG	4 cut(s)	321, 934, 1215, 1239
BstNSI	RCATGY	3 cut(s)	69, 364, 1008
BstSCI	CCNGG	7 cut(s)	148, 149, 319, 932, 1024, 1213, 1237
BstSFI	CTRYAG	2 cut(s)	26, 695
BstSLI	GKGCMC	2 cut(s)	781, 819
BstUI	CGCG	1 cut(s)	63
BstV1I	GCAGC	7 cut(s)	94, 220, 242, 296, 437, 587, 755
BstV2I	GAAGAC	2 cut(s)	25, 1251
BstX2I	RGATCY	2 cut(s)	480, 559
BstXI	CCANNNNNNTGG	1 cut(s)	320
BstYI	RGATCY	2 cut(s)	480, 559
BsuRI	GGCC	4 cut(s)	221, 817, 937, 1152
BtgI	CCRYGG	1 cut(s)	1402
BtsCI	GGATG	8 cut(s)	39, 115, 202, 892, 1174, 1248, 1312, 1442
BtsIMutI	CAGTG	3 cut(s)	38, 428, 922
Cac8I	GCNNGC	4 cut(s)	682, 741, 899, 1033
CaiI	CAGNNNCTG	1 cut(s)	484
CciI	TCATGA	1 cut(s)	385
CfoI	GCGC	2 cut(s)	65, 202
Cfr10I	RCCGGY	1 cut(s)	640
Cfr13I	GGNCC	3 cut(s)	815, 816, 1312
Cfr9I	CCCGGG	1 cut(s)	149
CseI	GACGC	1 cut(s)	66
Csp6I	GTAC	2 cut(s)	539, 1279
CspCI	CAANNNNNGTGG	2 cut(s)	108, 143
CviAII	CATG	9 cut(s)	66, 361, 367, 386, 617, 905, 1005, 1175, 1403
CviQI	GTAC	2 cut(s)	539, 1279
DdeI	CTNAG	2 cut(s)	747, 963
DpnI	GATC	6 cut(s)	482, 505, 561, 852, 921, 981
DpnII	GATC	6 cut(s)	480, 503, 559, 850, 919, 979
DraIII	CACNNNGTG	1 cut(s)	526
EaeI	YGGCCR	2 cut(s)	219, 935
Eam1104I	CTCTTC	2 cut(s)	961, 963
EarI	CTCTTC	2 cut(s)	961, 963
Ecl136II	GAGCTC	1 cut(s)	973
Eco130I	CCWWGG	1 cut(s)	1402
Eco24I	GRGCYC	2 cut(s)	819, 975
Eco47I	GGWCC	1 cut(s)	1312
Eco53kI	GAGCTC	1 cut(s)	973
Eco57I	CTGAAG	1 cut(s)	942
Eco88I	CYCGRG	1 cut(s)	149
EcoICRI	GAGCTC	1 cut(s)	973
EcoO109I	RGGNCCY	2 cut(s)	815, 1312
EcoRI	GAATTC	1 cut(s)	1095
EcoRII	CCWGG	4 cut(s)	319, 932, 1213, 1237
EcoT14I	CCWWGG	1 cut(s)	1402
EcoT38I	GRGCYC	2 cut(s)	819, 975
ErhI	CCWWGG	1 cut(s)	1402
FaeI	CATG	9 cut(s)	69, 364, 370, 389, 620, 908, 1008, 1178, 1406
FatI	CATG	9 cut(s)	65, 360, 366, 385, 616, 904, 1004, 1174, 1402
FauI	CCCGC	1 cut(s)	406
FbaI	TGATCA	1 cut(s)	850
Fnu4HI	GCNGC	8 cut(s)	61, 108, 231, 234, 310, 426, 576, 744
FokI	GGATG	8 cut(s)	46, 102, 209, 899, 1181, 1235, 1319, 1429
FriOI	GRGCYC	2 cut(s)	819, 975
Fsp4HI	GCNGC	8 cut(s)	61, 108, 231, 234, 310, 426, 576, 744
FspBI	CTAG	4 cut(s)	740, 825, 1032, 1110
GlaI	GCGC	2 cut(s)	64, 201
GluI	GCNGC	8 cut(s)	61, 108, 231, 234, 310, 426, 576, 744
HaeIII	GGCC	4 cut(s)	221, 817, 937, 1152
HapII	CCGG	3 cut(s)	150, 641, 1026
HgaI	GACGC	1 cut(s)	66
HhaI	GCGC	2 cut(s)	65, 202
Hin1I	GRCGYC	1 cut(s)	58
Hin1II	CATG	9 cut(s)	69, 364, 370, 389, 620, 908, 1008, 1178, 1406
Hin6I	GCGC	2 cut(s)	63, 200
HinP1I	GCGC	2 cut(s)	63, 200
HinfI	GANTC	8 cut(s)	382, 599, 665, 1080, 1106, 1135, 1242, 1379
HpaII	CCGG	3 cut(s)	150, 641, 1026
HphI	GGTGA	2 cut(s)	604, 630
Hpy188I	TCNGA	7 cut(s)	163, 511, 570, 846, 855, 1101, 1344
HpyAV	CCTTC	3 cut(s)	128, 205, 493
HpyCH4III	ACNGT	5 cut(s)	123, 627, 1189, 1202, 1268
HpyCH4IV	ACGT	2 cut(s)	390, 545
HpyF10VI	GCNNNNNNNGC	8 cut(s)	34, 227, 357, 452, 484, 677, 686, 1436
HpyF3I	CTNAG	2 cut(s)	747, 963
HpySE526I	ACGT	2 cut(s)	390, 545
Hsp92I	GRCGYC	1 cut(s)	58
Hsp92II	CATG	9 cut(s)	69, 364, 370, 389, 620, 908, 1008, 1178, 1406
HspAI	GCGC	2 cut(s)	63, 200
KpnI	GGTACC	1 cut(s)	1282
Ksp22I	TGATCA	1 cut(s)	850
Kzo9I	GATC	6 cut(s)	480, 503, 559, 850, 919, 979
LguI	GCTCTTC	1 cut(s)	963
LmnI	GCTCC	3 cut(s)	207, 1012, 1436
Lsp1109I	GCAGC	7 cut(s)	94, 220, 242, 296, 437, 587, 755
LweI	GCATC	6 cut(s)	24, 119, 187, 274, 680, 1296
MaeI	CTAG	4 cut(s)	740, 825, 1032, 1110
MaeII	ACGT	2 cut(s)	390, 545
MaeIII	GTNAC	2 cut(s)	621, 636
MalI	GATC	6 cut(s)	482, 505, 561, 852, 921, 981
MboI	GATC	6 cut(s)	480, 503, 559, 850, 919, 979
MboII	GAAGA	8 cut(s)	30, 88, 91, 192, 594, 948, 980, 1251
MfeI	CAATTG	2 cut(s)	675, 1230
MflI	RGATCY	2 cut(s)	480, 559
MhlI	GDGCHC	3 cut(s)	781, 819, 975
MlsI	TGGCCA	2 cut(s)	221, 937
MluCI	AATT	5 cut(s)	173, 554, 675, 1095, 1230
MluNI	TGGCCA	2 cut(s)	221, 937
MlyI	GAGTC	3 cut(s)	376, 674, 1074
MmeI	TCCRAC	3 cut(s)	73, 174, 1163
Mox20I	TGGCCA	2 cut(s)	221, 937
MroXI	GAANNNNTTC	2 cut(s)	1068, 1117
MscI	TGGCCA	2 cut(s)	221, 937
MseI	TTAA	4 cut(s)	629, 651, 656, 1368
MslI	CAYNNNNRTG	4 cut(s)	365, 726, 909, 1407
Msp20I	TGGCCA	2 cut(s)	221, 937
MspA1I	CMGCKG	2 cut(s)	233, 1132
MspCI	CTTAAG	1 cut(s)	650
MspI	CCGG	3 cut(s)	150, 641, 1026
MspR9I	CCNGG	7 cut(s)	150, 151, 321, 934, 1026, 1215, 1239
MunI	CAATTG	2 cut(s)	675, 1230
Mva1269I	GAATGC	4 cut(s)	353, 767, 791, 1035
MvaI	CCWGG	4 cut(s)	321, 934, 1215, 1239
MvnI	CGCG	1 cut(s)	63
MwoI	GCNNNNNNNGC	8 cut(s)	34, 227, 357, 452, 484, 677, 686, 1436
NciI	CCSGG	3 cut(s)	150, 151, 1026
NcoI	CCATGG	1 cut(s)	1402
NdeII	GATC	6 cut(s)	480, 503, 559, 850, 919, 979
NheI	GCTAGC	2 cut(s)	739, 1031
NlaIII	CATG	9 cut(s)	69, 364, 370, 389, 620, 908, 1008, 1178, 1406
NlaIV	GGNNCC	5 cut(s)	561, 778, 816, 817, 1280
NmuCI	GTSAC	1 cut(s)	636
NspI	RCATGY	3 cut(s)	69, 364, 1008
PagI	TCATGA	1 cut(s)	385
PciI	ACATGT	1 cut(s)	1004
PciSI	GCTCTTC	1 cut(s)	963
PctI	GAATGC	4 cut(s)	353, 767, 791, 1035
PdmI	GAANNNNTTC	2 cut(s)	1068, 1117
PfeI	GAWTC	5 cut(s)	599, 1106, 1135, 1242, 1379
PflFI	GACNNNGTC	1 cut(s)	1348
PflMI	CCANNNNNTGG	3 cut(s)	787, 944, 1159
PkrI	GCNGC	8 cut(s)	62, 109, 232, 235, 311, 427, 577, 745
PleI	GAGTC	3 cut(s)	376, 673, 1074
PpsI	GAGTC	3 cut(s)	376, 673, 1074
PpuMI	RGGWCCY	1 cut(s)	1312
PscI	ACATGT	1 cut(s)	1004
Psp124BI	GAGCTC	1 cut(s)	975
Psp5II	RGGWCCY	1 cut(s)	1312
Psp6I	CCWGG	4 cut(s)	319, 932, 1213, 1237
PspGI	CCWGG	4 cut(s)	319, 932, 1213, 1237
PspN4I	GGNNCC	5 cut(s)	561, 778, 816, 817, 1280
PspOMI	GGGCCC	1 cut(s)	815
PspPI	GGNCC	3 cut(s)	815, 816, 1312
PspPPI	RGGWCCY	1 cut(s)	1312
PstI	CTGCAG	2 cut(s)	30, 699
PstNI	CAGNNNCTG	1 cut(s)	484
PsuI	RGATCY	2 cut(s)	480, 559
PsyI	GACNNNGTC	1 cut(s)	1348
PvuII	CAGCTG	2 cut(s)	233, 1132
RsaI	GTAC	2 cut(s)	540, 1280
RsaNI	GTAC	2 cut(s)	539, 1279
RseI	CAYNNNNRTG	4 cut(s)	365, 726, 909, 1407
SacI	GAGCTC	1 cut(s)	975
SapI	GCTCTTC	1 cut(s)	963
SaqAI	TTAA	4 cut(s)	629, 651, 656, 1368
SatI	GCNGC	8 cut(s)	61, 108, 231, 234, 310, 426, 576, 744
Sau3AI	GATC	6 cut(s)	480, 503, 559, 850, 919, 979
Sau96I	GGNCC	3 cut(s)	815, 816, 1312
SchI	GAGTC	3 cut(s)	376, 674, 1074
ScrFI	CCNGG	7 cut(s)	150, 151, 321, 934, 1026, 1215, 1239
SduI	GDGCHC	3 cut(s)	781, 819, 975
SfaNI	GCATC	6 cut(s)	24, 119, 187, 274, 680, 1296
SfcI	CTRYAG	2 cut(s)	26, 695
SinI	GGWCC	1 cut(s)	1312
SmaI	CCCGGG	1 cut(s)	151
SmiMI	CAYNNNNRTG	4 cut(s)	365, 726, 909, 1407
SmlI	CTYRAG	2 cut(s)	650, 1082
SmoI	CTYRAG	2 cut(s)	650, 1082
Sse9I	AATT	5 cut(s)	173, 554, 675, 1095, 1230
SsiI	CCGC	3 cut(s)	61, 351, 413
SspMI	CTAG	4 cut(s)	740, 825, 1032, 1110
SstI	GAGCTC	1 cut(s)	975
StyD4I	CCNGG	7 cut(s)	148, 149, 319, 932, 1024, 1213, 1237
StyI	CCWWGG	1 cut(s)	1402
TaaI	ACNGT	5 cut(s)	123, 627, 1189, 1202, 1268
TaiI	ACGT	2 cut(s)	393, 548
TaqI	TCGA	1 cut(s)	1039
TaqII	GACCGA	1 cut(s)	269
TasI	AATT	5 cut(s)	173, 554, 675, 1095, 1230
TauI	GCSGC	1 cut(s)	63
TfiI	GAWTC	5 cut(s)	599, 1106, 1135, 1242, 1379
Tru1I	TTAA	4 cut(s)	629, 651, 656, 1368
Tru9I	TTAA	4 cut(s)	629, 651, 656, 1368
TscAI	CASTG	3 cut(s)	45, 435, 922
TseFI	GTSAC	1 cut(s)	636
TseI	GCWGC	7 cut(s)	107, 230, 233, 309, 425, 575, 743
Tsp45I	GTSAC	1 cut(s)	636
TspDTI	ATGAA	6 cut(s)	355, 479, 512, 774, 893, 1431
TspGWI	ACGGA	1 cut(s)	1194
TspMI	CCCGGG	1 cut(s)	149
TspRI	CASTG	3 cut(s)	45, 435, 922
Tth111I	GACNNNGTC	1 cut(s)	1348
Van91I	CCANNNNNTGG	3 cut(s)	787, 944, 1159
Vha464I	CTTAAG	1 cut(s)	650
VpaK11BI	GGWCC	1 cut(s)	1312
XapI	RAATTY	1 cut(s)	1095
XbaI	TCTAGA	1 cut(s)	1109
XceI	RCATGY	3 cut(s)	69, 364, 1008
XcmI	CCANNNNNNNNNTGG	1 cut(s)	320
XmaI	CCCGGG	1 cut(s)	149
XmnI	GAANNNNTTC	2 cut(s)	1068, 1117
XspI	CTAG	4 cut(s)	740, 825, 1032, 1110

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.