Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0006224

Physical Location & Seq

Genomic Coordinates Reverse (-)

43037324 .. 43044871

Gene Structure 7548 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025217

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1992 bp

ATGCGGATTCTTATTGGAAAACTTCTGCAGAGAATTTCATACAAGCAGACTATTGGACGCCGTGCATTTTGCACCAAGAACATTGTTGACATTGGGCAGCCAACTCCTTCATCTCATCCACAGTTGATGAAAGAAGGGGAGATTACGCCCGGCATATCTTTGGAGGAATACATATTGAGAAGAAAAAGGTTGTTGGATGTGCTCCCAGAGAATGGCTTGGCCATCTTTGCAGCTGCGCCGGTGAAGATGATGAATGATGTTGTTCCTTATACGTATCGGCAAGATGCTGATTACTCGTACATTACTGGCTGCCAGCAACCTGGTGGCGTGGCGGTCTTGGGTCATGAATGCGGTTTTTGCATGTTCATGCCTGAACCAACAACTCAAAAACATTCTGAAGTTCTTGGCTGGTGTGGTTTAACAGCTATCCTCCCAGATTTTATGAGGAGATCGTCCAAGCTGTTCCACAATGTGCAGACGGCTGTACCAACATATATGGAATTGGATGCCTTTCAGAAAGCAGCTACCTCTGGAGAAGTGAAAGATGTTTCGGTTCTCACACATCAGTTACGGTTGATAAAGTCATCGGCAGAGCTCAAGTTAATGAGAGAGTCTGCAGCAATTGCTTGCCAGGCTCTGTTGCAGACAATGTTACATTCAAAGATACATCCTTATGAGGGTATGCTCTCAGCTAAGATGGAATATGAATGCAAAATGAGGGGTGCCCAGAGAATGGCGTTCAACCCTGTGGTTGGTGGAGGGCCTAATGCTAGTGTAATACATTATTCTCGAAATGATCAGAAAATAAAGGAAGGAGATCTTGTTTTGATGGATGTTGGTTGCGAATTTCATGGTTATGTCAGTGATCTAACCCGCACCTGGCCACCCTGTGGTAGCTTTTCTTCAGCTCAAGAAGAGCTATATGATCTTATTCTGCAAACAAACAAGGAATGCCTGAAGCTTTGCAAGCCTGGTGCTACCATTCGGCAAATACACAACTTTTCTGTTGACATGCTTCAAAAAGGACTCAAGGAGATTGGGATTCTGAAGGAGGACTCTAGACGTAGTTCCCACTATCAGTTGAGCCGTTCCTACCATCAGCTAAACCCTACTTCCATAGGTCACTATCTAGGAATGGATGTCCATGATTGTTCTAAGGTCAGCTACGACCATCCTTTGACGTCCGGTGTTGTGATAACAATTGAACCAGGAATCTACATCCCATTGTTCTCTAATATTCCTGAGAGGTATCAAGGCATCGGGATAAGGATTGAGGATGAGGTCCTTATTACAGACACGGGTTGTGAGGTCCTAACTGGGTCAATTCCAAAAGAAGTTAAACACATTGAATCCTTGCTAAACAACTTTCATCATGGAACTGTAATGGAGAGCCACACCCACCCAGATCAGTTTGTGCAGATTCAGAGACGATTAGTTGGTGCCAATGGGAACAGCTGCAGTGGAAAGGGTACCCAATGTGCAAACATTGTTAAGCATTTTGGGTACACCCATCTCAGTGCTGGTGATCTTCTACGAGCTGAAAGGAATTCTGGTTCCGAAAATGGAACCATGATTTCAAACATGATCAAAGAAGGACAAATTGTTCCTGCAGTGGTGACGATTAAGCTTCTGGAGCGAGCAATGCTGGAGAGCGGTAATGACAAGTTTCTCATTGATGGGTTTCCTCGTAATGAAGAAAACCGTGCCGCATTTGAGGATGTGACTAAAATCGTGCCCTCATTCGTCCTGTATTTTAATTGTTCTGAAGAAGAGATGGAGAGGCGCCTTTTGAGTAGGAACCAGGGAAGAGAGGATGATAATGTAGAAACAATACGGAAGAGGTTTAAGGTTTTTCTGGAGTCAACTAAAAAAGTTTTAGAGTACTATAACGCCCAGGGGAAGGTCCGAGAGATTGATGCTGGGAGGCCTGTTGAAGAGGTTTTCGAGTCAGTGAAAGGCATTTTTGGCCCCAAAAATGAGAAGGCCGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003008 GO:0003014 GO:0003094 GO:0003674 GO:0003824 GO:0004177 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008235 GO:0008237 GO:0008238 GO:0010467 GO:0016485 GO:0016787 GO:0019538 GO:0030145 GO:0031647 GO:0032501 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0046983 GO:0050821 GO:0051604 GO:0065007 GO:0065008 GO:0070006 GO:0070011 GO:0071704 GO:0097205 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AMP_N Peptidase_M24

Protein Analysis

664

Amino Acids

74.38

Weight (kDa)

6.75

Isoelectric Point (pI)

45.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP_N	PF05195	57 - 129	7.1e-18	Aminopeptidase P, N-terminal domain
Peptidase_M24	PF00557	201 - 432	8.7e-53	Metallopeptidase family M24
AAA_33	PF13671	481 - 615	4.5e-06	AAA domain
ADK	PF00406	483 - 633	8.4e-41	Adenylate kinase
AAA_17	PF13207	485 - 612	4e-31	AAA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006224)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09300 AT1G09300
fragaria_vesca	FvH4_6g07800 FvH4_6g07800 FvH4_6g07800 FvH4_6g07800 FvH4_6g07800
malus_domestica	MD04G1191800.v1.1
prunus_persica	Prupe.6G310400_v2.0.a1 Prupe.6G310400_v2.0.a1 Prupe.6G310400_v2.0.a1 Prupe.6G310400_v2.0.a1 Prupe.6G310400_v2.0.a1
pyrus_communis	pycom12g19110 pycom12g19120
rosa_chinensis	RchiOBHm_Chr3g0457511
rosa_laevigata	RLG00000025217
rosa_multiflora	Rmu_sc0007404.1_g000015 Rmu_sc0038466.1_g000001
rosa_roxburghii	Rroxscaffold_6G00421810
rosa_rugosa	Rorug03G0020600 Rorug03G0020900
rosa_samantha	Rh3AG081400 Rh3BG083500 Rh3CG083700 Rh3DG085100
rosa_wichuraiana	Rw3G006980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1184
Acc65I	GGTACC	1 cut(s)	1469
AccB1I	GGYRCC	4 cut(s)	722, 1439, 1469, 1782
AccB7I	CCANNNNNTGG	3 cut(s)	212, 733, 890
AccBSI	CCGCTC	1 cut(s)	1653
AciI	CCGC	6 cut(s)	4, 332, 351, 874, 1653, 1707
AcoI	YGGCCR	2 cut(s)	219, 881
AcsI	RAATTY	3 cut(s)	33, 845, 1546
AcuI	CTGAAG	5 cut(s)	417, 888, 977, 1067, 1785
AcyI	GRCGYC	3 cut(s)	58, 1181, 1783
AdeI	CACNNNGTG	2 cut(s)	472, 890
AfaI	GTAC	5 cut(s)	299, 486, 1471, 1505, 1883
AfiI	CCNNNNNNNGG	7 cut(s)	212, 677, 733, 752, 879, 890, 1900
AgsI	TTSAA	7 cut(s)	660, 742, 1019, 1205, 1349, 1578, 1934
AjnI	CCWGG	7 cut(s)	319, 630, 878, 970, 1207, 1800, 1893
AloI	GAACNNNNNNTCC	4 cut(s)	1537, 1569, 1587, 1619
Alw21I	GWGCWC	2 cut(s)	204, 597
Alw26I	GTCTC	1 cut(s)	1420
AlwNI	CAGNNNCTG	1 cut(s)	637
AoxI	GGCC	6 cut(s)	219, 761, 881, 1925, 1966, 1983
ApeKI	GCWGC	7 cut(s)	97, 230, 233, 309, 521, 617, 1455
ApoI	RAATTY	3 cut(s)	33, 845, 1546
Asp718I	GGTACC	1 cut(s)	1469
AspLEI	GCGC	2 cut(s)	238, 1785
AspS9I	GGNCC	5 cut(s)	761, 1282, 1309, 1903, 1967
AsuC2I	CCSGG	1 cut(s)	150
AsuHPI	GGTGA	3 cut(s)	253, 1535, 1627
AvaII	GGWCC	3 cut(s)	1282, 1309, 1903
BaeGI	GKGCMC	2 cut(s)	727, 1737
BaeI	ACNNNNGTAYC	2 cut(s)	468, 501
BalI	TGGCCA	2 cut(s)	221, 883
BanI	GGYRCC	4 cut(s)	722, 1439, 1469, 1782
BanII	GRGCYC	1 cut(s)	597
BarI	GAAGNNNNNNTAC	2 cut(s)	886, 918
Bbv12I	GWGCWC	2 cut(s)	204, 597
BbvI	GCAGC	7 cut(s)	109, 220, 242, 296, 533, 629, 1442
BccI	CCATC	8 cut(s)	230, 691, 823, 1104, 1179, 1518, 1670, 1768
BceAI	ACGGC	3 cut(s)	45, 495, 1071
BciT130I	CCWGG	7 cut(s)	321, 632, 880, 972, 1209, 1802, 1895
BclI	TGATCA	2 cut(s)	796, 1584
BcnI	CCSGG	1 cut(s)	150
BcoDI	GTCTC	1 cut(s)	1420
BfaI	CTAG	3 cut(s)	771, 1059, 1130
BfmI	CTRYAG	4 cut(s)	26, 615, 1456, 1608
BfoI	RGCGCY	1 cut(s)	1786
BglII	AGATCT	1 cut(s)	817
BisI	GCNGC	8 cut(s)	98, 231, 234, 310, 522, 618, 1456, 1707
BlsI	GCNGC	8 cut(s)	99, 232, 235, 311, 523, 619, 1457, 1708
BmcAI	AGTACT	1 cut(s)	1883
Bme1390I	CCNGG	8 cut(s)	150, 321, 632, 880, 972, 1209, 1802, 1895
Bme18I	GGWCC	3 cut(s)	1282, 1309, 1903
BmgT120I	GGNCC	5 cut(s)	761, 1282, 1309, 1903, 1967
BmiI	GGNNCC	8 cut(s)	724, 1441, 1471, 1555, 1567, 1784, 1799, 1969
BmrFI	CCNGG	8 cut(s)	150, 321, 632, 880, 972, 1209, 1802, 1895
BmrI	ACTGGG	1 cut(s)	1326
BmsI	GCATC	4 cut(s)	274, 496, 1266, 1906
BmuI	ACTGGG	1 cut(s)	1326
BpmI	CTGGAG	4 cut(s)	552, 1652, 1667, 1877
BpuEI	CTTGAG	3 cut(s)	581, 894, 1013
BpuMI	CCSGG	1 cut(s)	150
BsaAI	YACGTR	1 cut(s)	273
BsaHI	GRCGYC	3 cut(s)	58, 1181, 1783
BsaJI	CCNNGG	3 cut(s)	1801, 1893, 1894
BsaWI	WCCGGW	1 cut(s)	1184
BsaXI	ACNNNNNCTCC	2 cut(s)	807, 837
Bsc4I	CCNNNNNNNGG	7 cut(s)	212, 677, 733, 752, 879, 890, 1900
Bse118I	RCCGGY	1 cut(s)	238
Bse1I	ACTGG	2 cut(s)	310, 1321
Bse3DI	GCAATG	1 cut(s)	1647
BseBI	CCWGG	7 cut(s)	321, 632, 880, 972, 1209, 1802, 1895
BseDI	CCNNGG	3 cut(s)	1801, 1893, 1894
BseLI	CCNNNNNNNGG	7 cut(s)	212, 677, 733, 752, 879, 890, 1900
BseMI	GCAATG	1 cut(s)	1647
BseMII	CTCAG	3 cut(s)	702, 1233, 1528
BseNI	ACTGG	2 cut(s)	310, 1321
BseRI	GAGGAG	1 cut(s)	460
BseSI	GKGCMC	2 cut(s)	727, 1737
BseXI	GCAGC	7 cut(s)	109, 220, 242, 296, 533, 629, 1442
BseYI	CCCAGC	1 cut(s)	1919
BsgI	GTGCAG	2 cut(s)	494, 1436
BshFI	GGCC	6 cut(s)	221, 763, 883, 1927, 1968, 1985
BshNI	GGYRCC	4 cut(s)	722, 1439, 1469, 1782
BsiHKAI	GWGCWC	2 cut(s)	204, 597
BsiSI	CCGG	3 cut(s)	150, 239, 1185
BslI	CCNNNNNNNGG	7 cut(s)	212, 677, 733, 752, 879, 890, 1900
BsmAI	GTCTC	1 cut(s)	1420
BsmBI	CGTCTC	1 cut(s)	1420
BsmI	GAATGC	3 cut(s)	353, 713, 956
BsnI	GGCC	6 cut(s)	221, 763, 883, 1927, 1968, 1985
Bsp1286I	GDGCHC	4 cut(s)	204, 597, 727, 1737
Bsp143I	GATC	8 cut(s)	449, 796, 817, 865, 925, 1405, 1525, 1584
BspACI	CCGC	6 cut(s)	4, 332, 351, 874, 1653, 1707
BspANI	GGCC	6 cut(s)	221, 763, 883, 1927, 1968, 1985
BspCNI	CTCAG	3 cut(s)	701, 1234, 1527
BspHI	TCATGA	1 cut(s)	343
BspLI	GGNNCC	8 cut(s)	724, 1441, 1471, 1555, 1567, 1784, 1799, 1969
BspMAI	CTGCAG	4 cut(s)	30, 619, 1460, 1612
BspQI	GCTCTTC	1 cut(s)	909
BspT107I	GGYRCC	4 cut(s)	722, 1439, 1469, 1782
BsrBI	CCGCTC	1 cut(s)	1653
BsrDI	GCAATG	1 cut(s)	1647
BsrFI	RCCGGY	1 cut(s)	238
BsrI	ACTGG	2 cut(s)	310, 1321
BssAI	RCCGGY	1 cut(s)	238
BssECI	CCNNGG	3 cut(s)	1801, 1893, 1894
BssMI	GATC	8 cut(s)	449, 796, 817, 865, 925, 1405, 1525, 1584
BssNI	GRCGYC	3 cut(s)	58, 1181, 1783
Bst2UI	CCWGG	7 cut(s)	321, 632, 880, 972, 1209, 1802, 1895
Bst4CI	ACNGT	4 cut(s)	123, 573, 1381, 1703
Bst6I	CTCTTC	5 cut(s)	909, 1764, 1801, 1832, 1929
BstACI	GRCGYC	3 cut(s)	58, 1181, 1783
BstAPI	GCANNNNNTGC	1 cut(s)	623
BstBAI	YACGTR	1 cut(s)	273
BstC8I	GCNNGC	4 cut(s)	314, 628, 968, 1638
BstDEI	CTNAG	5 cut(s)	688, 693, 1155, 1242, 1514
BstH2I	RGCGCY	1 cut(s)	1786
BstHHI	GCGC	2 cut(s)	238, 1785
BstKTI	GATC	8 cut(s)	452, 799, 820, 868, 928, 1408, 1528, 1587
BstMAI	GTCTC	1 cut(s)	1420
BstMBI	GATC	8 cut(s)	449, 796, 817, 865, 925, 1405, 1525, 1584
BstMWI	GCNNNNNNNGC	8 cut(s)	227, 357, 623, 632, 967, 1633, 1642, 1965
BstNI	CCWGG	7 cut(s)	321, 632, 880, 972, 1209, 1802, 1895
BstNSI	RCATGY	2 cut(s)	364, 1015
BstSCI	CCNGG	8 cut(s)	148, 319, 630, 878, 970, 1207, 1800, 1893
BstSFI	CTRYAG	4 cut(s)	26, 615, 1456, 1608
BstSLI	GKGCMC	2 cut(s)	727, 1737
BstSNI	TACGTA	1 cut(s)	273
BstV1I	GCAGC	7 cut(s)	109, 220, 242, 296, 533, 629, 1442
BstX2I	RGATCY	1 cut(s)	817
BstXI	CCANNNNNNTGG	1 cut(s)	320
BstYI	RGATCY	1 cut(s)	817
BsuRI	GGCC	6 cut(s)	221, 763, 883, 1927, 1968, 1985
BtsI	GCAGTG	2 cut(s)	1465, 1617
BtsIMutI	CAGTG	5 cut(s)	868, 1465, 1522, 1617, 1956
Cac8I	GCNNGC	4 cut(s)	314, 628, 968, 1638
CaiI	CAGNNNCTG	1 cut(s)	637
CciI	TCATGA	1 cut(s)	343
CfoI	GCGC	2 cut(s)	238, 1785
Cfr10I	RCCGGY	1 cut(s)	238
Cfr13I	GGNCC	5 cut(s)	761, 1282, 1309, 1903, 1967
CseI	GACGC	1 cut(s)	66
CsiI	ACCWGGT	1 cut(s)	319
Csp6I	GTAC	5 cut(s)	298, 485, 1470, 1504, 1882
CviAII	CATG	9 cut(s)	344, 361, 367, 851, 1012, 1145, 1373, 1570, 1582
CviQI	GTAC	5 cut(s)	298, 485, 1470, 1504, 1882
DdeI	CTNAG	5 cut(s)	688, 693, 1155, 1242, 1514
DinI	GGCGCC	1 cut(s)	1784
DpnI	GATC	8 cut(s)	451, 798, 819, 867, 927, 1407, 1527, 1586
DpnII	GATC	8 cut(s)	449, 796, 817, 865, 925, 1405, 1525, 1584
DraIII	CACNNNGTG	2 cut(s)	472, 890
EaeI	YGGCCR	2 cut(s)	219, 881
Eam1104I	CTCTTC	5 cut(s)	909, 1764, 1801, 1832, 1929
EarI	CTCTTC	5 cut(s)	909, 1764, 1801, 1832, 1929
Ecl136II	GAGCTC	1 cut(s)	595
Eco105I	TACGTA	1 cut(s)	273
Eco147I	AGGCCT	1 cut(s)	1927
Eco24I	GRGCYC	1 cut(s)	597
Eco47I	GGWCC	3 cut(s)	1282, 1309, 1903
Eco53kI	GAGCTC	1 cut(s)	595
Eco57I	CTGAAG	5 cut(s)	417, 888, 977, 1067, 1785
EcoICRI	GAGCTC	1 cut(s)	595
EcoO109I	RGGNCCY	3 cut(s)	761, 1282, 1309
EcoRI	GAATTC	1 cut(s)	1546
EcoRII	CCWGG	7 cut(s)	319, 630, 878, 970, 1207, 1800, 1893
EcoT38I	GRGCYC	1 cut(s)	597
EgeI	GGCGCC	1 cut(s)	1784
EheI	GGCGCC	1 cut(s)	1784
Esp3I	CGTCTC	1 cut(s)	1420
FaeI	CATG	9 cut(s)	347, 364, 370, 854, 1015, 1148, 1376, 1573, 1585
FalI	AAGNNNNNCTT	2 cut(s)	804, 836
FatI	CATG	9 cut(s)	343, 360, 366, 850, 1011, 1144, 1372, 1569, 1581
FauI	CCCGC	1 cut(s)	881
FbaI	TGATCA	2 cut(s)	796, 1584
Fnu4HI	GCNGC	8 cut(s)	98, 231, 234, 310, 522, 618, 1456, 1707
FriOI	GRGCYC	1 cut(s)	597
Fsp4HI	GCNGC	8 cut(s)	98, 231, 234, 310, 522, 618, 1456, 1707
FspBI	CTAG	3 cut(s)	771, 1059, 1130
GlaI	GCGC	2 cut(s)	237, 1784
GluI	GCNGC	8 cut(s)	98, 231, 234, 310, 522, 618, 1456, 1707
GsaI	CCCAGC	1 cut(s)	1923
GsuI	CTGGAG	4 cut(s)	552, 1652, 1667, 1877
HaeII	RGCGCY	1 cut(s)	1786
HaeIII	GGCC	6 cut(s)	221, 763, 883, 1927, 1968, 1985
HapII	CCGG	3 cut(s)	150, 239, 1185
HgaI	GACGC	1 cut(s)	66
HhaI	GCGC	2 cut(s)	238, 1785
Hin1I	GRCGYC	3 cut(s)	58, 1181, 1783
Hin1II	CATG	9 cut(s)	347, 364, 370, 854, 1015, 1148, 1376, 1573, 1585
Hin6I	GCGC	2 cut(s)	236, 1783
HinP1I	GCGC	2 cut(s)	236, 1783
HincII	GTYRAC	3 cut(s)	88, 1009, 1863
HindII	GTYRAC	3 cut(s)	88, 1009, 1863
HindIII	AAGCTT	2 cut(s)	959, 1625
HpaII	CCGG	3 cut(s)	150, 239, 1185
HphI	GGTGA	3 cut(s)	253, 1535, 1627
Hpy166II	GTNNAC	4 cut(s)	88, 1009, 1506, 1863
Hpy188I	TCNGA	8 cut(s)	397, 516, 801, 1047, 1425, 1558, 1765, 1907
Hpy188III	TCNNGA	9 cut(s)	344, 531, 789, 911, 1059, 1241, 1261, 1631, 1856
Hpy8I	GTNNAC	4 cut(s)	88, 1009, 1506, 1863
HpyAV	CCTTC	7 cut(s)	117, 128, 806, 1042, 1586, 1894, 1975
HpyCH4III	ACNGT	4 cut(s)	123, 573, 1381, 1703
HpyCH4IV	ACGT	3 cut(s)	272, 1063, 1181
HpyF10VI	GCNNNNNNNGC	8 cut(s)	227, 357, 623, 632, 967, 1633, 1642, 1965
HpyF3I	CTNAG	5 cut(s)	688, 693, 1155, 1242, 1514
HpySE526I	ACGT	3 cut(s)	272, 1063, 1181
Hsp92I	GRCGYC	3 cut(s)	58, 1181, 1783
Hsp92II	CATG	9 cut(s)	347, 364, 370, 854, 1015, 1148, 1376, 1573, 1585
HspAI	GCGC	2 cut(s)	236, 1783
KasI	GGCGCC	1 cut(s)	1782
KpnI	GGTACC	1 cut(s)	1473
Ksp22I	TGATCA	2 cut(s)	796, 1584
Kzo9I	GATC	8 cut(s)	449, 796, 817, 865, 925, 1405, 1525, 1584
LguI	GCTCTTC	1 cut(s)	909
LmnI	GCTCC	2 cut(s)	207, 1633
Lsp1109I	GCAGC	7 cut(s)	109, 220, 242, 296, 533, 629, 1442
LweI	GCATC	4 cut(s)	274, 496, 1266, 1906
MabI	ACCWGGT	1 cut(s)	319
MaeI	CTAG	3 cut(s)	771, 1059, 1130
MaeII	ACGT	3 cut(s)	272, 1063, 1181
MaeIII	GTNAC	5 cut(s)	567, 651, 1121, 1615, 1720
MalI	GATC	8 cut(s)	451, 798, 819, 867, 927, 1407, 1527, 1586
MbiI	CCGCTC	1 cut(s)	1653
MboI	GATC	8 cut(s)	449, 796, 817, 865, 925, 1405, 1525, 1584
MfeI	CAATTG	2 cut(s)	621, 1200
MflI	RGATCY	1 cut(s)	817
MhlI	GDGCHC	4 cut(s)	204, 597, 727, 1737
MlsI	TGGCCA	2 cut(s)	221, 883
MluCI	AATT	9 cut(s)	33, 500, 621, 845, 1200, 1323, 1546, 1599, 1756
MluNI	TGGCCA	2 cut(s)	221, 883
Mly113I	GGCGCC	1 cut(s)	1783
MlyI	GAGTC	5 cut(s)	620, 1020, 1049, 1868, 1955
MmeI	TCCRAC	1 cut(s)	174
Mox20I	TGGCCA	2 cut(s)	221, 883
MscI	TGGCCA	2 cut(s)	221, 883
MseI	TTAA	7 cut(s)	419, 602, 1338, 1491, 1623, 1755, 1845
MslI	CAYNNNNRTG	4 cut(s)	365, 672, 855, 1515
Msp20I	TGGCCA	2 cut(s)	221, 883
MspA1I	CMGCKG	2 cut(s)	233, 1455
MspI	CCGG	3 cut(s)	150, 239, 1185
MspR9I	CCNGG	8 cut(s)	150, 321, 632, 880, 972, 1209, 1802, 1895
MunI	CAATTG	2 cut(s)	621, 1200
Mva1269I	GAATGC	3 cut(s)	353, 713, 956
MvaI	CCWGG	7 cut(s)	321, 632, 880, 972, 1209, 1802, 1895
MwoI	GCNNNNNNNGC	8 cut(s)	227, 357, 623, 632, 967, 1633, 1642, 1965
NarI	GGCGCC	1 cut(s)	1783
NciI	CCSGG	1 cut(s)	150
NdeII	GATC	8 cut(s)	449, 796, 817, 865, 925, 1405, 1525, 1584
NlaIII	CATG	9 cut(s)	347, 364, 370, 854, 1015, 1148, 1376, 1573, 1585
NlaIV	GGNNCC	8 cut(s)	724, 1441, 1471, 1555, 1567, 1784, 1799, 1969
NmuCI	GTSAC	3 cut(s)	1121, 1615, 1720
NspI	RCATGY	2 cut(s)	364, 1015
PagI	TCATGA	1 cut(s)	343
PasI	CCCWGGG	1 cut(s)	1894
PceI	AGGCCT	1 cut(s)	1927
PciSI	GCTCTTC	1 cut(s)	909
PctI	GAATGC	3 cut(s)	353, 713, 956
PfeI	GAWTC	5 cut(s)	7, 1042, 1212, 1349, 1420
PflMI	CCANNNNNTGG	3 cut(s)	212, 733, 890
PkrI	GCNGC	8 cut(s)	99, 232, 235, 311, 523, 619, 1457, 1708
PleI	GAGTC	5 cut(s)	619, 1020, 1049, 1867, 1954
PluTI	GGCGCC	1 cut(s)	1786
PpsI	GAGTC	5 cut(s)	619, 1020, 1049, 1867, 1954
Ppu21I	YACGTR	1 cut(s)	273
PpuMI	RGGWCCY	2 cut(s)	1282, 1309
Psp124BI	GAGCTC	1 cut(s)	597
Psp5II	RGGWCCY	2 cut(s)	1282, 1309
Psp6I	CCWGG	7 cut(s)	319, 630, 878, 970, 1207, 1800, 1893
PspFI	CCCAGC	1 cut(s)	1919
PspGI	CCWGG	7 cut(s)	319, 630, 878, 970, 1207, 1800, 1893
PspN4I	GGNNCC	8 cut(s)	724, 1441, 1471, 1555, 1567, 1784, 1799, 1969
PspPI	GGNCC	5 cut(s)	761, 1282, 1309, 1903, 1967
PspPPI	RGGWCCY	2 cut(s)	1282, 1309
PstI	CTGCAG	4 cut(s)	30, 619, 1460, 1612
PstNI	CAGNNNCTG	1 cut(s)	637
PsuI	RGATCY	1 cut(s)	817
PvuII	CAGCTG	2 cut(s)	233, 1455
RsaI	GTAC	5 cut(s)	299, 486, 1471, 1505, 1883
RsaNI	GTAC	5 cut(s)	298, 485, 1470, 1504, 1882
RseI	CAYNNNNRTG	4 cut(s)	365, 672, 855, 1515
SacI	GAGCTC	1 cut(s)	597
SapI	GCTCTTC	1 cut(s)	909
SaqAI	TTAA	7 cut(s)	419, 602, 1338, 1491, 1623, 1755, 1845
SatI	GCNGC	8 cut(s)	98, 231, 234, 310, 522, 618, 1456, 1707
Sau3AI	GATC	8 cut(s)	449, 796, 817, 865, 925, 1405, 1525, 1584
Sau96I	GGNCC	5 cut(s)	761, 1282, 1309, 1903, 1967
ScaI	AGTACT	1 cut(s)	1883
SchI	GAGTC	5 cut(s)	620, 1020, 1049, 1868, 1955
ScrFI	CCNGG	8 cut(s)	150, 321, 632, 880, 972, 1209, 1802, 1895
SduI	GDGCHC	4 cut(s)	204, 597, 727, 1737
SexAI	ACCWGGT	1 cut(s)	319
SfaNI	GCATC	4 cut(s)	274, 496, 1266, 1906
SfcI	CTRYAG	4 cut(s)	26, 615, 1456, 1608
SfoI	GGCGCC	1 cut(s)	1784
SgrAI	CRCCGGYG	1 cut(s)	238
SinI	GGWCC	3 cut(s)	1282, 1309, 1903
SmiMI	CAYNNNNRTG	4 cut(s)	365, 672, 855, 1515
SmlI	CTYRAG	3 cut(s)	596, 909, 1028
SmoI	CTYRAG	3 cut(s)	596, 909, 1028
SnaBI	TACGTA	1 cut(s)	273
Sse9I	AATT	9 cut(s)	33, 500, 621, 845, 1200, 1323, 1546, 1599, 1756
SseBI	AGGCCT	1 cut(s)	1927
SsiI	CCGC	6 cut(s)	4, 332, 351, 874, 1653, 1707
SspDI	GGCGCC	1 cut(s)	1782
SspI	AATATT	1 cut(s)	1237
SspMI	CTAG	3 cut(s)	771, 1059, 1130
SstI	GAGCTC	1 cut(s)	597
StuI	AGGCCT	1 cut(s)	1927
StyD4I	CCNGG	8 cut(s)	148, 319, 630, 878, 970, 1207, 1800, 1893
TaaI	ACNGT	4 cut(s)	123, 573, 1381, 1703
TaiI	ACGT	3 cut(s)	275, 1066, 1184
TaqI	TCGA	2 cut(s)	790, 1944
TasI	AATT	9 cut(s)	33, 500, 621, 845, 1200, 1323, 1546, 1599, 1756
TatI	WGTACW	1 cut(s)	1881
TauI	GCSGC	1 cut(s)	1709
TfiI	GAWTC	5 cut(s)	7, 1042, 1212, 1349, 1420
Tru1I	TTAA	7 cut(s)	419, 602, 1338, 1491, 1623, 1755, 1845
Tru9I	TTAA	7 cut(s)	419, 602, 1338, 1491, 1623, 1755, 1845
TscAI	CASTG	5 cut(s)	868, 1465, 1522, 1617, 1956
TseFI	GTSAC	3 cut(s)	1121, 1615, 1720
TseI	GCWGC	7 cut(s)	97, 230, 233, 309, 521, 617, 1455
Tsp45I	GTSAC	3 cut(s)	1121, 1615, 1720
TspGWI	ACGGA	1 cut(s)	1849
TspRI	CASTG	5 cut(s)	868, 1465, 1522, 1617, 1956
Van91I	CCANNNNNTGG	3 cut(s)	212, 733, 890
VpaK11BI	GGWCC	3 cut(s)	1282, 1309, 1903
XapI	RAATTY	3 cut(s)	33, 845, 1546
XbaI	TCTAGA	1 cut(s)	1058
XceI	RCATGY	2 cut(s)	364, 1015
XcmI	CCANNNNNNNNNTGG	2 cut(s)	320, 1517
XspI	CTAG	3 cut(s)	771, 1059, 1130
ZraI	GACGTC	1 cut(s)	1182
ZrmI	AGTACT	1 cut(s)	1883

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.