pycom12g19410

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007517
Physical Location & Seq
Forward (+)
21281835 .. 21282392
558 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 558 bp
ATGCCGGTTTACTGGGATCAAGGAATAACCCGAGAAATGATAAGTTTTGCGGATTTCTTAGGAAGAGAACAACTTCCTAGACTTGTTTCTGTTGCTGGAGCAGCACAAGCAAATCCAACTATTTCTTGTCAAAGCTTCAATGTATACAATCAAGGATTAGGAAGCCCTAATCTTGACTCCCAATGGCTGAGTAATATCGAAAACCAGCTAATGATCGACTTACAAATCAAATTTAGCGATAAACAAGTCATGCCCCAGGCCAACTCACTCATTTCATTCCCAGAGCAAGTGAATTATGCAGTAGCAGCTGATATAAAGCACCCAGATTATGTACAAGAAATGGCTGATAAACGAAACTTGGTTTCTGGCGCAAATGACCTACTTCAGCCTCAACCGAACAGCTGGGTGATGCCACCACCTCAGCCAGGCAGCTGGGTGGCTGCCCGGAACTCAGGGATACAAATAACCGAGAACTACTCAACGTCTTCATCGTTCACTCAAAATTTGACTGTGGAAGAATTTGCAACTTACAACAACTTGGCCAAGCGCTTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

20.66

Weight (kDa)

4.77

Isoelectric Point (pI)

51.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 144
AciI CCGC 1 cut(s) 50
AclWI GGATC 1 cut(s) 24
AcoI YGGCCR 1 cut(s) 540
AcsI RAATTY 3 cut(s) 230, 502, 518
AcuI CTGAAG 1 cut(s) 368
AfaI GTAC 1 cut(s) 333
AfeI AGCGCT 1 cut(s) 548
AfiI CCNNNNNNNGG 1 cut(s) 425
AgsI TTSAA 2 cut(s) 139, 553
AjnI CCWGG 2 cut(s) 255, 424
AluBI AGCT 5 cut(s) 135, 208, 308, 402, 432
AluI AGCT 5 cut(s) 135, 208, 308, 402, 432
AlwI GGATC 1 cut(s) 24
Ama87I CYCGRG 1 cut(s) 30
Aor51HI AGCGCT 1 cut(s) 548
AoxI GGCC 2 cut(s) 258, 540
ApeKI GCWGC 4 cut(s) 101, 305, 429, 440
ApoI RAATTY 3 cut(s) 230, 502, 518
Asp700I GAANNNNTTC 1 cut(s) 72
AspLEI GCGC 2 cut(s) 371, 549
AsuC2I CCSGG 1 cut(s) 445
AsuHPI GGTGA 1 cut(s) 418
AvaI CYCGRG 1 cut(s) 30
BalI TGGCCA 1 cut(s) 542
BbsI GAAGAC 1 cut(s) 477
BbvCI CCTCAGC 1 cut(s) 420
BbvI GCAGC 4 cut(s) 113, 317, 427, 441
BciT130I CCWGG 2 cut(s) 257, 426
BciVI GTATCC 1 cut(s) 450
BcnI CCSGG 1 cut(s) 445
BfaI CTAG 1 cut(s) 78
BfoI RGCGCY 1 cut(s) 550
BfuI GTATCC 1 cut(s) 450
BisI GCNGC 4 cut(s) 102, 306, 430, 441
BlsI GCNGC 4 cut(s) 103, 307, 431, 442
Bme1390I CCNGG 3 cut(s) 257, 426, 445
BmeT110I CYCGRG 1 cut(s) 30
BmrFI CCNGG 3 cut(s) 257, 426, 445
BmrI ACTGGG 1 cut(s) 22
BmsI GCATC 1 cut(s) 399
BmuI ACTGGG 1 cut(s) 22
BpiI GAAGAC 1 cut(s) 477
BplI GAGNNNNNCTC 2 cut(s) 461, 493
BpmI CTGGAG 1 cut(s) 117
Bpu10I CCTNAGC 1 cut(s) 420
BpuMI CCSGG 1 cut(s) 445
BsaJI CCNNGG 1 cut(s) 255
Bsc4I CCNNNNNNNGG 1 cut(s) 425
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 1 cut(s) 17
BseBI CCWGG 2 cut(s) 257, 426
BseDI CCNNGG 1 cut(s) 255
BseLI CCNNNNNNNGG 1 cut(s) 425
BseMII CTCAG 3 cut(s) 179, 434, 465
BseNI ACTGG 1 cut(s) 17
BseXI GCAGC 4 cut(s) 113, 317, 427, 441
BseYI CCCAGC 2 cut(s) 402, 432
BshFI GGCC 2 cut(s) 260, 542
BsiHKCI CYCGRG 1 cut(s) 30
BsiSI CCGG 2 cut(s) 5, 445
BslI CCNNNNNNNGG 1 cut(s) 425
BsnI GGCC 2 cut(s) 260, 542
BsoBI CYCGRG 1 cut(s) 30
Bsp1407I TGTACA 1 cut(s) 331
Bsp143I GATC 2 cut(s) 16, 213
BspACI CCGC 1 cut(s) 50
BspANI GGCC 2 cut(s) 260, 542
BspCNI CTCAG 3 cut(s) 180, 433, 464
BspPI GGATC 1 cut(s) 24
BsrFI RCCGGY 1 cut(s) 4
BsrGI TGTACA 1 cut(s) 331
BsrI ACTGG 1 cut(s) 17
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 2 cut(s) 16, 213
BssNAI GTATAC 1 cut(s) 145
Bst1107I GTATAC 1 cut(s) 145
Bst2UI CCWGG 2 cut(s) 257, 426
Bst4CI ACNGT 1 cut(s) 511
Bst6I CTCTTC 1 cut(s) 58
BstAUI TGTACA 1 cut(s) 331
BstDEI CTNAG 4 cut(s) 58, 188, 420, 451
BstENI CCTNNNNNAGG 1 cut(s) 423
BstH2I RGCGCY 1 cut(s) 550
BstHHI GCGC 2 cut(s) 371, 549
BstKTI GATC 2 cut(s) 19, 216
BstMBI GATC 2 cut(s) 16, 213
BstMWI GCNNNNNNNGC 3 cut(s) 101, 107, 305
BstNI CCWGG 2 cut(s) 257, 426
BstSCI CCNGG 3 cut(s) 255, 424, 443
BstV1I GCAGC 4 cut(s) 113, 317, 427, 441
BstV2I GAAGAC 1 cut(s) 477
BstXI CCANNNNNNTGG 1 cut(s) 432
BstZ17I GTATAC 1 cut(s) 145
BsuI GTATCC 1 cut(s) 450
BsuRI GGCC 2 cut(s) 260, 542
CfoI GCGC 2 cut(s) 371, 549
Cfr10I RCCGGY 1 cut(s) 4
Csp6I GTAC 1 cut(s) 332
CviAII CATG 1 cut(s) 250
CviQI GTAC 1 cut(s) 332
DdeI CTNAG 4 cut(s) 58, 188, 420, 451
DpnI GATC 2 cut(s) 18, 215
DpnII GATC 2 cut(s) 16, 213
EaeI YGGCCR 1 cut(s) 540
Eam1104I CTCTTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 58
Eco47III AGCGCT 1 cut(s) 548
Eco57I CTGAAG 1 cut(s) 368
Eco88I CYCGRG 1 cut(s) 30
EcoNI CCTNNNNNAGG 1 cut(s) 423
EcoRII CCWGG 2 cut(s) 255, 424
FaeI CATG 1 cut(s) 253
FaiI YATR 5 cut(s) 145, 251, 297, 314, 330
FatI CATG 1 cut(s) 249
FblI GTMKAC 1 cut(s) 144
Fnu4HI GCNGC 4 cut(s) 102, 306, 430, 441
Fsp4HI GCNGC 4 cut(s) 102, 306, 430, 441
FspBI CTAG 1 cut(s) 78
GlaI GCGC 2 cut(s) 370, 548
GluI GCNGC 4 cut(s) 102, 306, 430, 441
GsaI CCCAGC 2 cut(s) 406, 436
GsuI CTGGAG 1 cut(s) 117
HaeII RGCGCY 1 cut(s) 550
HaeIII GGCC 2 cut(s) 260, 542
HapII CCGG 2 cut(s) 5, 445
HhaI GCGC 2 cut(s) 371, 549
Hin1II CATG 1 cut(s) 253
Hin6I GCGC 2 cut(s) 369, 547
HinP1I GCGC 2 cut(s) 369, 547
HindIII AAGCTT 1 cut(s) 133
HinfI GANTC 1 cut(s) 176
HpaII CCGG 2 cut(s) 5, 445
HphI GGTGA 1 cut(s) 418
Hpy166II GTNNAC 3 cut(s) 10, 145, 495
Hpy188III TCNNGA 1 cut(s) 173
Hpy8I GTNNAC 3 cut(s) 10, 145, 495
HpyCH4III ACNGT 1 cut(s) 511
HpyCH4IV ACGT 1 cut(s) 482
HpyCH4V TGCA 2 cut(s) 299, 524
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 107, 305
HpyF3I CTNAG 4 cut(s) 58, 188, 420, 451
HpySE526I ACGT 1 cut(s) 482
Hsp92II CATG 1 cut(s) 253
HspAI GCGC 2 cut(s) 369, 547
Kzo9I GATC 2 cut(s) 16, 213
LmnI GCTCC 1 cut(s) 98
Lsp1109I GCAGC 4 cut(s) 113, 317, 427, 441
LweI GCATC 1 cut(s) 399
MaeI CTAG 1 cut(s) 78
MaeII ACGT 1 cut(s) 482
MalI GATC 2 cut(s) 18, 215
MboI GATC 2 cut(s) 16, 213
MboII GAAGA 3 cut(s) 75, 477, 527
MlsI TGGCCA 1 cut(s) 542
MluCI AATT 4 cut(s) 230, 292, 502, 518
MluNI TGGCCA 1 cut(s) 542
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 1 cut(s) 140
MnlI CCTC 2 cut(s) 399, 429
Mox20I TGGCCA 1 cut(s) 542
MroXI GAANNNNTTC 1 cut(s) 72
MscI TGGCCA 1 cut(s) 542
Msp20I TGGCCA 1 cut(s) 542
MspA1I CMGCKG 3 cut(s) 308, 402, 432
MspI CCGG 2 cut(s) 5, 445
MspR9I CCNGG 3 cut(s) 257, 426, 445
MvaI CCWGG 2 cut(s) 257, 426
MwoI GCNNNNNNNGC 3 cut(s) 101, 107, 305
NciI CCSGG 1 cut(s) 445
NdeII GATC 2 cut(s) 16, 213
NlaIII CATG 1 cut(s) 253
PcsI WCGNNNNNNNCGW 1 cut(s) 488
PdmI GAANNNNTTC 1 cut(s) 72
PkrI GCNGC 4 cut(s) 103, 307, 431, 442
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
Psp6I CCWGG 2 cut(s) 255, 424
PspFI CCCAGC 2 cut(s) 402, 432
PspGI CCWGG 2 cut(s) 255, 424
PvuII CAGCTG 3 cut(s) 308, 402, 432
RsaI GTAC 1 cut(s) 333
RsaNI GTAC 1 cut(s) 332
SatI GCNGC 4 cut(s) 102, 306, 430, 441
Sau3AI GATC 2 cut(s) 16, 213
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 3 cut(s) 257, 426, 445
SetI ASST 8 cut(s) 137, 210, 310, 381, 404, 421, 434, 485
SfaNI GCATC 1 cut(s) 399
Sse9I AATT 4 cut(s) 230, 292, 502, 518
SsiI CCGC 1 cut(s) 50
SspMI CTAG 1 cut(s) 78
StyD4I CCNGG 3 cut(s) 255, 424, 443
TaaI ACNGT 1 cut(s) 511
TaiI ACGT 1 cut(s) 485
TaqI TCGA 2 cut(s) 198, 216
TasI AATT 4 cut(s) 230, 292, 502, 518
TatI WGTACW 1 cut(s) 331
TseI GCWGC 4 cut(s) 101, 305, 429, 440
TspDTI ATGAA 2 cut(s) 264, 477
XagI CCTNNNNNAGG 1 cut(s) 423
XapI RAATTY 3 cut(s) 230, 502, 518
XmiI GTMKAC 1 cut(s) 144
XmnI GAANNNNTTC 1 cut(s) 72
XspI CTAG 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.