pycom12g20550

Protein YIPF1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010775
Physical Location & Seq
Forward (+)
22023489 .. 22026398
2910 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g20550.2

Sequence Viewer

Length: 816 bp
ATGATGTCCGGAAATTATACCAGCATTGACAACCAGAACGTCTCGGGATCTGTTCCTGCTGCGGTTCCAGATCCAGGCCACCTCACCGTCAAATTCCAAGATTCAAGCCTTCAGACATTTCCTCCATCTGGGACACAAGGGAAGATCGCTGGTGGTGCCCTCCCTCCTCGTGATGCCGATGACACATTTTCTAAACCTGTATCTGGTCCTGATGAACCCCAGCAAGGTGGTTGGTTACGGGTATTCTCTGTAGCTTCTTACAAGCAATACTTTGATGTTGACACTTCAGATGTCCTAGAGAGGATTAAGGATTCACTTCTTCCATTCAGTGGAGCCTTCAATGAGAAAACATCTAACAGCCCAGATTTGTATGGACCTTTCTGGATATGCACTACCCTAATATTTGTAGCAGCTGCAATTGGCACTTTCGTGACGTATGTAGCACACAAGGTCAAGAGTAAAGATTGGGAATATGACATTAATGTGGTGCAATGGTCAGCGGGATTGTTCTATGGTTATGTCACTATTGTCCCTCTTGTACTGTATGTAATTCTCAAGTACTTCTCGGCACCATCAGGTCTCGTTCAGCTATTTTGTCTCTATGGTTACTCTCTGTTTGTCTTCATTCCAGCATTGTGCCTCTCAATTGTGCCTCTGGAAATCTTCAGATGGGTAATTGCAGGCATAGCTGGGGTTATGTCTGCCACCTTTGTCGCAGTTAATCTTCGCGCCCACATCGTATCAGCAGGTGAAAGGTGGTTCTTGATTGTAGCTGATCGATCTTTCTGTTGCAGTTGGCTCTGTCCCTCGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Yip1

Protein Analysis

272

Amino Acids

29.65

Weight (kDa)

5.3

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 737
Acc36I ACCTGC 1 cut(s) 737
AccB1I GGYRCC 2 cut(s) 155, 568
AccB7I CCANNNNNTGG 1 cut(s) 329
AccII CGCG 1 cut(s) 729
AccIII TCCGGA 1 cut(s) 8
AciI CCGC 2 cut(s) 62, 500
AclWI GGATC 2 cut(s) 55, 65
AcsI RAATTY 1 cut(s) 92
AcuI CTGAAG 3 cut(s) 95, 270, 649
AfaI GTAC 2 cut(s) 540, 560
AfiI CCNNNNNNNGG 5 cut(s) 74, 128, 203, 224, 329
AgsI TTSAA 2 cut(s) 105, 340
AjnI CCWGG 1 cut(s) 73
AleI CACNNNNGTG 1 cut(s) 428
AluBI AGCT 5 cut(s) 254, 413, 589, 689, 773
AluI AGCT 5 cut(s) 254, 413, 589, 689, 773
Alw26I GTCTC 3 cut(s) 46, 584, 602
AlwI GGATC 2 cut(s) 55, 65
Ama87I CYCGRG 1 cut(s) 43
Aor13HI TCCGGA 1 cut(s) 8
AoxI GGCC 1 cut(s) 76
ApeKI GCWGC 3 cut(s) 59, 410, 413
ApoI RAATTY 1 cut(s) 92
AseI ATTAAT 1 cut(s) 480
AspLEI GCGC 1 cut(s) 731
AspS9I GGNCC 2 cut(s) 206, 374
AsuHPI GGTGA 2 cut(s) 76, 761
AvaI CYCGRG 1 cut(s) 43
AvaII GGWCC 2 cut(s) 206, 374
BaeGI GKGCMC 1 cut(s) 160
BanI GGYRCC 2 cut(s) 155, 568
BauI CACGAG 1 cut(s) 168
BbsI GAAGAC 1 cut(s) 613
BbvI GCAGC 3 cut(s) 46, 400, 422
BccI CCATC 3 cut(s) 133, 580, 663
BciT130I CCWGG 1 cut(s) 75
BcoDI GTCTC 3 cut(s) 46, 584, 602
BfaI CTAG 1 cut(s) 296
BfmI CTRYAG 1 cut(s) 249
BfuAI ACCTGC 1 cut(s) 737
BisI GCNGC 3 cut(s) 60, 411, 414
BlsI GCNGC 3 cut(s) 61, 412, 415
BmcAI AGTACT 1 cut(s) 560
Bme1390I CCNGG 1 cut(s) 75
Bme18I GGWCC 2 cut(s) 206, 374
BmeT110I CYCGRG 1 cut(s) 43
BmgT120I GGNCC 2 cut(s) 206, 374
BmiI GGNNCC 4 cut(s) 66, 157, 334, 570
BmrFI CCNGG 1 cut(s) 75
BmsI GCATC 1 cut(s) 163
BpiI GAAGAC 1 cut(s) 613
BpuEI CTTGAG 1 cut(s) 539
Bsa29I ATCGAT 1 cut(s) 778
BsaI GGTCTC 1 cut(s) 584
BsaWI WCCGGW 1 cut(s) 8
BsaXI ACNNNNNCTCC 2 cut(s) 106, 136
Bsc4I CCNNNNNNNGG 5 cut(s) 74, 128, 203, 224, 329
Bse3DI GCAATG 1 cut(s) 497
BseAI TCCGGA 1 cut(s) 8
BseBI CCWGG 1 cut(s) 75
BseCI ATCGAT 1 cut(s) 778
BseLI CCNNNNNNNGG 5 cut(s) 74, 128, 203, 224, 329
BseMI GCAATG 1 cut(s) 497
BseRI GAGGAG 1 cut(s) 156
BseSI GKGCMC 1 cut(s) 160
BseXI GCAGC 3 cut(s) 46, 400, 422
BseYI CCCAGC 2 cut(s) 219, 689
Bsh1236I CGCG 1 cut(s) 729
BshFI GGCC 1 cut(s) 78
BshNI GGYRCC 2 cut(s) 155, 568
BshVI ATCGAT 1 cut(s) 778
BsiHKCI CYCGRG 1 cut(s) 43
BsiSI CCGG 1 cut(s) 9
BslFI GGGAC 3 cut(s) 145, 515, 789
BslI CCNNNNNNNGG 5 cut(s) 74, 128, 203, 224, 329
BsmAI GTCTC 3 cut(s) 46, 584, 602
BsmBI CGTCTC 1 cut(s) 46
BsmFI GGGAC 3 cut(s) 145, 515, 789
BsnI GGCC 1 cut(s) 78
Bso31I GGTCTC 1 cut(s) 584
BsoBI CYCGRG 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 160
Bsp13I TCCGGA 1 cut(s) 8
Bsp143I GATC 5 cut(s) 47, 70, 144, 775, 779
BspACI CCGC 2 cut(s) 62, 500
BspANI GGCC 1 cut(s) 78
BspDI ATCGAT 1 cut(s) 778
BspEI TCCGGA 1 cut(s) 8
BspFNI CGCG 1 cut(s) 729
BspLI GGNNCC 4 cut(s) 66, 157, 334, 570
BspMI ACCTGC 1 cut(s) 737
BspPI GGATC 2 cut(s) 55, 65
BspT107I GGYRCC 2 cut(s) 155, 568
BspTNI GGTCTC 1 cut(s) 584
BsrDI GCAATG 1 cut(s) 497
BssMI GATC 5 cut(s) 47, 70, 144, 775, 779
BssSI CACGAG 1 cut(s) 168
Bst2BI CACGAG 1 cut(s) 168
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 2 cut(s) 88, 543
BstC8I GCNNGC 1 cut(s) 682
BstFNI CGCG 1 cut(s) 729
BstHHI GCGC 1 cut(s) 731
BstKTI GATC 5 cut(s) 50, 73, 147, 778, 782
BstMAI GTCTC 3 cut(s) 46, 584, 602
BstMBI GATC 5 cut(s) 47, 70, 144, 775, 779
BstMWI GCNNNNNNNGC 2 cut(s) 155, 686
BstNI CCWGG 1 cut(s) 75
BstSCI CCNGG 1 cut(s) 73
BstSFI CTRYAG 1 cut(s) 249
BstSLI GKGCMC 1 cut(s) 160
BstUI CGCG 1 cut(s) 729
BstV1I GCAGC 3 cut(s) 46, 400, 422
BstV2I GAAGAC 1 cut(s) 613
BstX2I RGATCY 2 cut(s) 47, 70
BstXI CCANNNNNNTGG 1 cut(s) 227
BstYI RGATCY 2 cut(s) 47, 70
Bsu15I ATCGAT 1 cut(s) 778
BsuRI GGCC 1 cut(s) 78
BsuTUI ATCGAT 1 cut(s) 778
BtsIMutI CAGTG 1 cut(s) 334
BveI ACCTGC 1 cut(s) 737
Cac8I GCNNGC 1 cut(s) 682
CfoI GCGC 1 cut(s) 731
Cfr13I GGNCC 2 cut(s) 206, 374
ClaI ATCGAT 1 cut(s) 778
Csp6I GTAC 2 cut(s) 539, 559
CviQI GTAC 2 cut(s) 539, 559
DpnI GATC 5 cut(s) 49, 72, 146, 777, 781
DpnII GATC 5 cut(s) 47, 70, 144, 775, 779
Eco31I GGTCTC 1 cut(s) 584
Eco47I GGWCC 2 cut(s) 206, 374
Eco57I CTGAAG 3 cut(s) 95, 270, 649
Eco88I CYCGRG 1 cut(s) 43
EcoRII CCWGG 1 cut(s) 73
Esp3I CGTCTC 1 cut(s) 46
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FaqI GGGAC 3 cut(s) 145, 515, 789
FauI CCCGC 1 cut(s) 493
Fnu4HI GCNGC 3 cut(s) 60, 411, 414
Fsp4HI GCNGC 3 cut(s) 60, 411, 414
FspBI CTAG 1 cut(s) 296
GlaI GCGC 1 cut(s) 730
GluI GCNGC 3 cut(s) 60, 411, 414
GsaI CCCAGC 2 cut(s) 223, 693
HaeIII GGCC 1 cut(s) 78
HapII CCGG 1 cut(s) 9
HhaI GCGC 1 cut(s) 731
Hin6I GCGC 1 cut(s) 729
HinP1I GCGC 1 cut(s) 729
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 2 cut(s) 101, 311
HpaII CCGG 1 cut(s) 9
HphI GGTGA 2 cut(s) 76, 761
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 3 cut(s) 114, 289, 668
Hpy8I GTNNAC 1 cut(s) 280
HpyAV CCTTC 2 cut(s) 119, 346
HpyCH4III ACNGT 2 cut(s) 88, 543
HpyCH4IV ACGT 2 cut(s) 39, 434
HpyCH4V TGCA 5 cut(s) 390, 416, 490, 680, 792
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 686
HpySE526I ACGT 2 cut(s) 39, 434
HspAI GCGC 1 cut(s) 729
Kpn2I TCCGGA 1 cut(s) 8
Kzo9I GATC 5 cut(s) 47, 70, 144, 775, 779
LmnI GCTCC 1 cut(s) 332
Lsp1109I GCAGC 3 cut(s) 46, 400, 422
LweI GCATC 1 cut(s) 163
MaeI CTAG 1 cut(s) 296
MaeII ACGT 2 cut(s) 39, 434
MaeIII GTNAC 4 cut(s) 234, 430, 520, 605
MalI GATC 5 cut(s) 49, 72, 146, 777, 781
MboI GATC 5 cut(s) 47, 70, 144, 775, 779
MboII GAAGA 5 cut(s) 154, 311, 613, 655, 716
MfeI CAATTG 2 cut(s) 417, 645
MflI RGATCY 2 cut(s) 47, 70
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 6 cut(s) 13, 92, 417, 549, 645, 675
MnlI CCTC 9 cut(s) 92, 132, 170, 174, 177, 294, 543, 650, 663
MroI TCCGGA 1 cut(s) 8
MseI TTAA 3 cut(s) 306, 480, 720
MslI CAYNNNNRTG 2 cut(s) 428, 482
MspA1I CMGCKG 2 cut(s) 413, 500
MspI CCGG 1 cut(s) 9
MspR9I CCNGG 1 cut(s) 75
MunI CAATTG 2 cut(s) 417, 645
MvaI CCWGG 1 cut(s) 75
MvnI CGCG 1 cut(s) 729
MwoI GCNNNNNNNGC 2 cut(s) 155, 686
NdeII GATC 5 cut(s) 47, 70, 144, 775, 779
NlaIV GGNNCC 4 cut(s) 66, 157, 334, 570
NmeAIII GCCGAG 1 cut(s) 545
NmuCI GTSAC 2 cut(s) 430, 520
OliI CACNNNNGTG 1 cut(s) 428
PaqCI CACCTGC 1 cut(s) 737
PfeI GAWTC 2 cut(s) 101, 311
PflMI CCANNNNNTGG 1 cut(s) 329
PkrI GCNGC 3 cut(s) 61, 412, 415
PshBI ATTAAT 1 cut(s) 480
Psp6I CCWGG 1 cut(s) 73
PspFI CCCAGC 2 cut(s) 219, 689
PspGI CCWGG 1 cut(s) 73
PspN4I GGNNCC 4 cut(s) 66, 157, 334, 570
PspPI GGNCC 2 cut(s) 206, 374
PsuI RGATCY 2 cut(s) 47, 70
PvuII CAGCTG 1 cut(s) 413
RsaI GTAC 2 cut(s) 540, 560
RsaNI GTAC 2 cut(s) 539, 559
RseI CAYNNNNRTG 2 cut(s) 428, 482
SaqAI TTAA 3 cut(s) 306, 480, 720
SatI GCNGC 3 cut(s) 60, 411, 414
Sau3AI GATC 5 cut(s) 47, 70, 144, 775, 779
Sau96I GGNCC 2 cut(s) 206, 374
ScaI AGTACT 1 cut(s) 560
ScrFI CCNGG 1 cut(s) 75
SduI GDGCHC 1 cut(s) 160
SfaNI GCATC 1 cut(s) 163
SfcI CTRYAG 1 cut(s) 249
SinI GGWCC 2 cut(s) 206, 374
SmiMI CAYNNNNRTG 2 cut(s) 428, 482
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
Sse9I AATT 6 cut(s) 13, 92, 417, 549, 645, 675
SsiI CCGC 2 cut(s) 62, 500
SspI AATATT 1 cut(s) 402
SspMI CTAG 1 cut(s) 296
StyD4I CCNGG 1 cut(s) 73
TaaI ACNGT 2 cut(s) 88, 543
TaiI ACGT 2 cut(s) 42, 437
TaqI TCGA 1 cut(s) 778
TasI AATT 6 cut(s) 13, 92, 417, 549, 645, 675
TatI WGTACW 2 cut(s) 538, 558
TfiI GAWTC 2 cut(s) 101, 311
Tru1I TTAA 3 cut(s) 306, 480, 720
Tru9I TTAA 3 cut(s) 306, 480, 720
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 2 cut(s) 430, 520
TseI GCWGC 3 cut(s) 59, 410, 413
Tsp45I GTSAC 2 cut(s) 430, 520
TspDTI ATGAA 2 cut(s) 228, 613
TspRI CASTG 1 cut(s) 334
Van91I CCANNNNNTGG 1 cut(s) 329
VpaK11BI GGWCC 2 cut(s) 206, 374
VspI ATTAAT 1 cut(s) 480
XapI RAATTY 1 cut(s) 92
XspI CTAG 1 cut(s) 296
ZrmI AGTACT 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.