pycom12g21300

Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0006833
Physical Location & Seq
Reverse (-)
22500937 .. 22501804
868 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 495 bp
ATGCCTTGCTTGTATGTGCCTACGAGTGATGTTCACAATGGACAGATTCATTGTGCACCGAGAAGTTTTTTTGGGGTAGAGGATTTCCTCGATGATGACAATAGCCGCCCATACACTTACCAGAAGGAGAAGTCCAAAAATCCAACAAAACATATATCATTTAAACAGCGCACCGTAGCCTACATTGAACCGTTTACACTGGATGTGTTCATCTCAAAGCGGTTTGTGTCAGCCTCGATCACCCACAGGGTCACAACCAAGCAGGTTGCAACTGCGGGTACCAACTCCAAAGACATTAAAGCTGTACTCAAGTCACGTTGCGACATACCTGCCTGTTTGGCGGTCGGGCAAATTTTAGCCTATAGGGCAAGAGAAGCCGATGTTTATACTGCTGCTTGTACTCCCCGGGACAGGGACAAGTTTGAAGGGAAGATTAGAGCAGTTGTTCAATCCCTCATTGATAATGGGATTGATGTTAAAGTTTATCTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

165

Amino Acids

18.44

Weight (kDa)

8.95

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 46 - 158 2.3e-07 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 253, 337
Acc65I GGTACC 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 278
AciI CCGC 4 cut(s) 106, 220, 275, 341
AcsI RAATTY 1 cut(s) 351
AfaI GTAC 3 cut(s) 280, 306, 400
AfiI CCNNNNNNNGG 2 cut(s) 411, 412
AgsI TTSAA 3 cut(s) 188, 425, 449
AluBI AGCT 1 cut(s) 302
AluI AGCT 1 cut(s) 302
Alw21I GWGCWC 1 cut(s) 58
Alw44I GTGCAC 1 cut(s) 54
Ama87I CYCGRG 1 cut(s) 405
ApaLI GTGCAC 1 cut(s) 54
ApeKI GCWGC 1 cut(s) 392
ApoI RAATTY 1 cut(s) 351
Asp718I GGTACC 1 cut(s) 278
AspLEI GCGC 1 cut(s) 171
AsuC2I CCSGG 2 cut(s) 406, 407
AsuHPI GGTGA 1 cut(s) 232
AvaI CYCGRG 1 cut(s) 405
BaeGI GKGCMC 1 cut(s) 58
BanI GGYRCC 1 cut(s) 278
Bbv12I GWGCWC 1 cut(s) 58
BbvI GCAGC 1 cut(s) 379
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BcnI CCSGG 2 cut(s) 406, 407
BfmI CTRYAG 1 cut(s) 361
BfuAI ACCTGC 2 cut(s) 253, 337
BglI GCCNNNNNGGC 2 cut(s) 338, 365
BisI GCNGC 2 cut(s) 106, 393
BlsI GCNGC 2 cut(s) 107, 394
Bme1390I CCNGG 2 cut(s) 406, 407
BmeT110I CYCGRG 1 cut(s) 405
BmiI GGNNCC 1 cut(s) 280
BmrFI CCNGG 2 cut(s) 406, 407
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 2 cut(s) 406, 407
BsaJI CCNNGG 2 cut(s) 404, 405
Bsc4I CCNNNNNNNGG 2 cut(s) 411, 412
Bse1I ACTGG 1 cut(s) 204
BseDI CCNNGG 2 cut(s) 404, 405
BseGI GGATG 1 cut(s) 208
BseLI CCNNNNNNNGG 2 cut(s) 411, 412
BseNI ACTGG 1 cut(s) 204
BseSI GKGCMC 1 cut(s) 58
BseXI GCAGC 1 cut(s) 379
Bsh1285I CGRYCG 1 cut(s) 345
BshNI GGYRCC 1 cut(s) 278
BsiEI CGRYCG 1 cut(s) 345
BsiHKAI GWGCWC 1 cut(s) 58
BsiHKCI CYCGRG 1 cut(s) 405
BsiSI CCGG 1 cut(s) 406
BslFI GGGAC 2 cut(s) 422, 428
BslI CCNNNNNNNGG 2 cut(s) 411, 412
BsmFI GGGAC 2 cut(s) 422, 428
BsoBI CYCGRG 1 cut(s) 405
Bsp1286I GDGCHC 1 cut(s) 58
Bsp143I GATC 1 cut(s) 237
BspACI CCGC 4 cut(s) 106, 220, 275, 341
BspLI GGNNCC 1 cut(s) 280
BspMI ACCTGC 2 cut(s) 253, 337
BspT107I GGYRCC 1 cut(s) 278
BsrI ACTGG 1 cut(s) 204
BssECI CCNNGG 2 cut(s) 404, 405
BssMI GATC 1 cut(s) 237
Bst4CI ACNGT 2 cut(s) 175, 192
BstF5I GGATG 1 cut(s) 208
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 1 cut(s) 240
BstMBI GATC 1 cut(s) 237
BstMCI CGRYCG 1 cut(s) 345
BstMWI GCNNNNNNNGC 3 cut(s) 338, 365, 374
BstSCI CCNGG 2 cut(s) 404, 405
BstSFI CTRYAG 1 cut(s) 361
BstSLI GKGCMC 1 cut(s) 58
BstV1I GCAGC 1 cut(s) 379
BtsCI GGATG 1 cut(s) 208
BtsIMutI CAGTG 1 cut(s) 197
BveI ACCTGC 2 cut(s) 253, 337
CfoI GCGC 1 cut(s) 171
Cfr9I CCCGGG 1 cut(s) 405
Csp6I GTAC 3 cut(s) 279, 305, 399
CviJI RGCY 6 cut(s) 105, 179, 233, 302, 359, 377
CviKI_1 RGCY 6 cut(s) 105, 179, 233, 302, 359, 377
CviQI GTAC 3 cut(s) 279, 305, 399
DpnI GATC 1 cut(s) 239
DpnII GATC 1 cut(s) 237
DraI TTTAAA 1 cut(s) 163
Eco88I CYCGRG 1 cut(s) 405
FaiI YATR 7 cut(s) 15, 112, 153, 155, 326, 363, 387
FalI AAGNNNNNCTT 1 cut(s) 471
FaqI GGGAC 2 cut(s) 422, 428
FauI CCCGC 1 cut(s) 268
Fnu4HI GCNGC 2 cut(s) 106, 393
FokI GGATG 1 cut(s) 215
Fsp4HI GCNGC 2 cut(s) 106, 393
GlaI GCGC 1 cut(s) 170
GluI GCNGC 2 cut(s) 106, 393
HapII CCGG 1 cut(s) 406
HhaI GCGC 1 cut(s) 171
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HinfI GANTC 1 cut(s) 46
HpaII CCGG 1 cut(s) 406
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 3 cut(s) 34, 56, 195
Hpy188III TCNNGA 1 cut(s) 488
Hpy8I GTNNAC 3 cut(s) 34, 56, 195
HpyAV CCTTC 2 cut(s) 118, 419
HpyCH4III ACNGT 2 cut(s) 175, 192
HpyCH4IV ACGT 1 cut(s) 316
HpyCH4V TGCA 2 cut(s) 56, 269
HpyF10VI GCNNNNNNNGC 3 cut(s) 338, 365, 374
HpySE526I ACGT 1 cut(s) 316
HspAI GCGC 1 cut(s) 169
KpnI GGTACC 1 cut(s) 282
Kzo9I GATC 1 cut(s) 237
LpnPI CCDG 8 cut(s) 134, 185, 232, 248, 342, 346, 397, 419
Lsp1109I GCAGC 1 cut(s) 379
MaeII ACGT 1 cut(s) 316
MaeIII GTNAC 2 cut(s) 250, 312
MalI GATC 1 cut(s) 239
MboI GATC 1 cut(s) 237
MboII GAAGA 1 cut(s) 442
MhlI GDGCHC 1 cut(s) 58
MluCI AATT 1 cut(s) 351
MmeI TCCRAC 1 cut(s) 167
MnlI CCTC 4 cut(s) 73, 98, 244, 464
MseI TTAA 3 cut(s) 162, 297, 477
MspI CCGG 1 cut(s) 406
MspR9I CCNGG 2 cut(s) 406, 407
MwoI GCNNNNNNNGC 3 cut(s) 338, 365, 374
NciI CCSGG 2 cut(s) 406, 407
NdeII GATC 1 cut(s) 237
NlaIV GGNNCC 1 cut(s) 280
NmuCI GTSAC 2 cut(s) 250, 312
PfeI GAWTC 1 cut(s) 46
PkrI GCNGC 2 cut(s) 107, 394
PspN4I GGNNCC 1 cut(s) 280
RsaI GTAC 3 cut(s) 280, 306, 400
RsaNI GTAC 3 cut(s) 279, 305, 399
SaqAI TTAA 3 cut(s) 162, 297, 477
SatI GCNGC 2 cut(s) 106, 393
Sau3AI GATC 1 cut(s) 237
ScrFI CCNGG 2 cut(s) 406, 407
SduI GDGCHC 1 cut(s) 58
SetI ASST 4 cut(s) 267, 304, 319, 331
SfcI CTRYAG 1 cut(s) 361
SmaI CCCGGG 1 cut(s) 407
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 1 cut(s) 351
SsiI CCGC 4 cut(s) 106, 220, 275, 341
StyD4I CCNGG 2 cut(s) 404, 405
TaaI ACNGT 2 cut(s) 175, 192
TaiI ACGT 1 cut(s) 319
TaqI TCGA 2 cut(s) 90, 236
TasI AATT 1 cut(s) 351
TatI WGTACW 2 cut(s) 304, 398
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 1 cut(s) 46
Tru1I TTAA 3 cut(s) 162, 297, 477
Tru9I TTAA 3 cut(s) 162, 297, 477
TscAI CASTG 1 cut(s) 204
TseFI GTSAC 2 cut(s) 250, 312
TseI GCWGC 1 cut(s) 392
Tsp45I GTSAC 2 cut(s) 250, 312
TspDTI ATGAA 2 cut(s) 38, 199
TspMI CCCGGG 1 cut(s) 405
TspRI CASTG 1 cut(s) 204
VneI GTGCAC 1 cut(s) 54
XapI RAATTY 1 cut(s) 351
XmaI CCCGGG 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.