pycom13g01840

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009426
Physical Location & Seq
Reverse (-)
1236491 .. 1239543
3053 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2007 bp
ATGACCTATATCAGTCACAGTTTTCCTCAACACCGACAATATCTATGTGCTGGTGCATGGATCCTGATGCAAGGGCACCCAGAAAACATTAACAGCGTAAACTTAGCACGTGTCTTGAGAGAGTTTTCTCCTGAGGAAGTAACATCTAATATTTATACAATGGTGGATGTTCTGCTTCATCAAATTCAGTTGGAACTTCAGCATGGCCATTCTTTTCAGGATCTTTTATTGAAAGCTTGTGCAAACCTAGCTTTTTATATTTGGACCCATGAGTTGCTCCCTTTGGATATTTTGCTCCTGGCACTTATTGATCGTGACGATGACCCCCATGCTTTGCATATCGTGATTAGTCTACTCGATAGGCAAGAACTTCAACAGAGGGTGAAACTATATTGTGCGAATCGAGGTCCCCCTGAACACTGGCTTTTCTCTGGACCCTTTAAGCGAGTTGAATTGCAAAAGGCCCTTGGGAATCATCTATCCTGGAAGGACAGGTATCCTACTTTCTTCGATGATATTGCAGGGCGTTTGCTCCCAGTCATTCCGTTGATAATTTATAGACTAATTGAAAATGATGCCATAGATTCAGCTGAAAGAATTCTGGTCATGTATTCTACATTTCTTAAATACCACCCTTTAAGATTTACATTTGTTCGTGACATACTTGCATATTTTTATGGCCATCTTCCCGGAAAGCTCATTGTCCGAATATTGAATGGACTCGAAGTCAACAAGATCCCATTTTCTGAGTCATTCCCGGTGCATATGAATTTATCAAATTCCACCATGTGCCCACCTGCCGATTACTTTGCAACTCTCTTACTGGGACTAGTGAATAATGTCATTCCTCCATTGCATAACAACTCAAAATCTGGAGCGAATGATGCTTTAAATAATTCAATGCGTGCCCCACCTAACAAGACTCCTCCAACGTCGCAGTCTGGGCAAGCAAATGTTTCTGATGGCCAAAAAGCATTCTTTCAAATTCAGGATCGAGGCACATATACTCAGCTGGTTCTTGAAACAGCAGTCATTGAACTGCTCTCTCTTCCTGTATCTTCATCCCAGATTGTATCATCATTGGTTCAGATTGTCATCAATATACAACCAACATTAATTCAATCCAGCAATGGCCTGCACGGAGCTCCAAATGGTGTTGGGCAAGGCTCTGTTTTACCAACATCCCCTTCGGGCGGAAGCACAGATTCCTTGGGCACGAGCAGGTCAGCTGCTTCAGGGTCAGGAATAAATGCATCTAACTTTGTTTCTCGAAGTGGTTATACATGCCAACAGTTGTCTTGCTTCAACAAGGGTAGATTGATCCAAGCATGTGGTCTTCTACTGGCTCAACTGCCTCCTGATTTTCATATGCAACTCTATCTAGAGGCATCACGTATAATAAAAGAAACATGGTGGCTCACTGATGGCAAAGGGTCAATGGGAGAAGTAGACTCTGCTGTCGGTTATGCTTTGTTGGATCCAACATGGGCTGCTCAAGACAATACCTCGACCGCCATTGGTAATATTGTTGCCCTGCTTCATTCGTTTTTCAGTAACCTCCCACATGAGTGGCTAGAAGGGACCCATCTTATCATCAAACATCTCAGGCCAGTGACTTCAGTTGCAATGTTGAGAATAGCATTCCGGATAATGGGTCCCTTGCTCCCTAAACTAGCCACTGCGCACGCCCTCTTCAGTAAGGCTCTGTCGTTGATTTTAAGTATGATGGTAGATGTCTTCGGGAAGAATGTACAGCCTCCAATTCCAGTCAAGCCATTAGAAATAGCAGATATAATTGACTTCTTCCATCACATCGTCCACTATGAAGGGCAGGGAGGTCCTGTGCAGGCTAACAGCAAGCCCAGACCAGAGGTTTTAGCTCTCTGTGGAAGAGCCGCGGAAAGTCTGCGTCCGGACATACAACACCTTCTTTCCCACTTAAAACCCGACACGAATTCTTCCATTTACGCTGCTACCCATCCCAAGTTGAACCAGAATTCGTCGTAA

Protein Analysis

669

Amino Acids

73.89

Weight (kDa)

6.73

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med23 PF11573 28 - 278 5e-14 Mediator complex subunit 23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 805
AasI GACNNNNNNGTC 1 cut(s) 1457
Acc16I TGCGCA 1 cut(s) 1683
Acc36I ACCTGC 2 cut(s) 805, 1212
AccB1I GGYRCC 1 cut(s) 75
AccI GTMKAC 2 cut(s) 352, 1449
AccII CGCG 1 cut(s) 1898
AccIII TCCGGA 2 cut(s) 1644, 1912
AciI CCGC 4 cut(s) 1194, 1512, 1896, 1898
AclWI GGATC 8 cut(s) 55, 68, 228, 730, 999, 1315, 1472, 1485
AcoI YGGCCR 3 cut(s) 205, 679, 964
AcsI RAATTY 7 cut(s) 183, 597, 769, 778, 984, 1954, 1996
AcuI CTGAAG 4 cut(s) 182, 1218, 1602, 1678
AcvI CACGTG 1 cut(s) 110
AdeI CACNNNGTG 1 cut(s) 789
AfaI GTAC 1 cut(s) 1752
AfiI CCNNNNNNNGG 2 cut(s) 1193, 1651
AflIII ACRYGT 1 cut(s) 109
AhdI GACNNNNNGTC 1 cut(s) 725
AhlI ACTAGT 1 cut(s) 829
AjnI CCWGG 2 cut(s) 297, 482
AjuI GAANNNNNNNTTGG 2 cut(s) 1171, 1203
AleI CACNNNNGTG 1 cut(s) 1567
AluBI AGCT 8 cut(s) 236, 251, 590, 697, 1012, 1145, 1229, 1880
AluI AGCT 8 cut(s) 236, 251, 590, 697, 1012, 1145, 1229, 1880
Alw21I GWGCWC 1 cut(s) 1147
AlwI GGATC 8 cut(s) 55, 68, 228, 730, 999, 1315, 1472, 1485
Aor13HI TCCGGA 2 cut(s) 1644, 1912
AoxI GGCC 6 cut(s) 205, 462, 679, 964, 1132, 1607
ApeKI GCWGC 3 cut(s) 1229, 1490, 1970
ApoI RAATTY 7 cut(s) 183, 597, 769, 778, 984, 1954, 1996
AseI ATTAAT 1 cut(s) 1115
Asp700I GAANNNNTTC 1 cut(s) 597
AspLEI GCGC 1 cut(s) 1684
AspS9I GGNCC 7 cut(s) 264, 407, 434, 463, 1581, 1655, 1838
AsuC2I CCSGG 2 cut(s) 690, 758
AsuHPI GGTGA 1 cut(s) 394
AvaII GGWCC 6 cut(s) 264, 407, 434, 1581, 1655, 1838
AxyI CCTNAGG 1 cut(s) 132
BaeGI GKGCMC 4 cut(s) 78, 794, 910, 1217
BalI TGGCCA 3 cut(s) 207, 681, 966
BamHI GGATCC 2 cut(s) 60, 1477
BanI GGYRCC 1 cut(s) 75
BanII GRGCYC 1 cut(s) 1147
BarI GAAGNNNNNNTAC 2 cut(s) 1264, 1296
BauI CACGAG 1 cut(s) 1216
BbrPI CACGTG 1 cut(s) 110
BbsI GAAGAC 2 cut(s) 1328, 1729
Bbv12I GWGCWC 1 cut(s) 1147
BbvI GCAGC 3 cut(s) 1216, 1477, 1957
BccI CCATC 7 cut(s) 690, 956, 1418, 1593, 1720, 1815, 1986
BcgI CGANNNNNNTGC 4 cut(s) 500, 534, 791, 825
BciT130I CCWGG 2 cut(s) 299, 484
BciVI GTATCC 1 cut(s) 507
BcnI CCSGG 2 cut(s) 690, 758
BcuI ACTAGT 1 cut(s) 829
BfaI CTAG 5 cut(s) 248, 830, 1382, 1574, 1673
BfuAI ACCTGC 2 cut(s) 805, 1212
BfuI GTATCC 1 cut(s) 507
BisI GCNGC 4 cut(s) 1230, 1491, 1896, 1971
BlsI GCNGC 4 cut(s) 1231, 1492, 1897, 1972
Bme1390I CCNGG 4 cut(s) 299, 484, 690, 758
Bme18I GGWCC 6 cut(s) 264, 407, 434, 1581, 1655, 1838
BmeRI GACNNNNNGTC 1 cut(s) 725
BmgT120I GGNCC 7 cut(s) 264, 407, 434, 463, 1581, 1655, 1838
BmrFI CCNGG 4 cut(s) 299, 484, 690, 758
BmrI ACTGGG 2 cut(s) 530, 833
BmsI GCATC 5 cut(s) 57, 565, 874, 1262, 1397
BmuI ACTGGG 2 cut(s) 530, 833
BpiI GAAGAC 2 cut(s) 1328, 1729
BpmI CTGGAG 1 cut(s) 894
BpuEI CTTGAG 2 cut(s) 136, 1479
BpuMI CCSGG 2 cut(s) 690, 758
BsaAI YACGTR 2 cut(s) 110, 1394
BsaBI GATNNNNATC 1 cut(s) 1094
BsaJI CCNNGG 3 cut(s) 466, 1209, 1896
BsaWI WCCGGW 2 cut(s) 1644, 1912
BsaXI ACNNNNNCTCC 2 cut(s) 1827, 1857
Bsc4I CCNNNNNNNGG 2 cut(s) 1193, 1651
Bse1I ACTGG 6 cut(s) 425, 536, 828, 1347, 1610, 1766
Bse21I CCTNAGG 1 cut(s) 132
Bse3DI GCAATG 3 cut(s) 851, 1135, 1632
Bse8I GATNNNNATC 1 cut(s) 1094
BseAI TCCGGA 2 cut(s) 1644, 1912
BseBI CCWGG 2 cut(s) 299, 484
BseDI CCNNGG 3 cut(s) 466, 1209, 1896
BseGI GGATG 4 cut(s) 172, 1061, 1181, 1978
BseJI GATNNNNATC 1 cut(s) 1094
BseLI CCNNNNNNNGG 2 cut(s) 1193, 1651
BseMI GCAATG 3 cut(s) 851, 1135, 1632
BseMII CTCAG 4 cut(s) 123, 738, 1022, 1618
BseNI ACTGG 6 cut(s) 425, 536, 828, 1347, 1610, 1766
BseRI GAGGAG 1 cut(s) 915
BseSI GKGCMC 4 cut(s) 78, 794, 910, 1217
BseXI GCAGC 3 cut(s) 1216, 1477, 1957
BsgI GTGCAG 2 cut(s) 1121, 1865
Bsh1236I CGCG 1 cut(s) 1898
Bsh1285I CGRYCG 1 cut(s) 1512
BshFI GGCC 6 cut(s) 207, 464, 681, 966, 1134, 1609
BshNI GGYRCC 1 cut(s) 75
BsiEI CGRYCG 1 cut(s) 1512
BsiHKAI GWGCWC 1 cut(s) 1147
BsiSI CCGG 4 cut(s) 690, 758, 1645, 1913
BslFI GGGAC 4 cut(s) 393, 840, 1594, 1641
BslI CCNNNNNNNGG 2 cut(s) 1193, 1651
BsmFI GGGAC 4 cut(s) 393, 840, 1594, 1641
BsmI GAATGC 2 cut(s) 974, 1640
BsnI GGCC 6 cut(s) 207, 464, 681, 966, 1134, 1609
Bsp1286I GDGCHC 5 cut(s) 78, 794, 910, 1147, 1217
Bsp13I TCCGGA 2 cut(s) 1644, 1912
Bsp1407I TGTACA 1 cut(s) 1750
Bsp143I GATC 7 cut(s) 60, 220, 310, 735, 991, 1320, 1477
BspACI CCGC 4 cut(s) 1194, 1512, 1896, 1898
BspANI GGCC 6 cut(s) 207, 464, 681, 966, 1134, 1609
BspCNI CTCAG 4 cut(s) 124, 739, 1021, 1617
BspEI TCCGGA 2 cut(s) 1644, 1912
BspFNI CGCG 1 cut(s) 1898
BspMI ACCTGC 2 cut(s) 805, 1212
BspPI GGATC 8 cut(s) 55, 68, 228, 730, 999, 1315, 1472, 1485
BspQI GCTCTTC 1 cut(s) 1885
BspT107I GGYRCC 1 cut(s) 75
BsrDI GCAATG 3 cut(s) 851, 1135, 1632
BsrGI TGTACA 1 cut(s) 1750
BsrI ACTGG 6 cut(s) 425, 536, 828, 1347, 1610, 1766
BssECI CCNNGG 3 cut(s) 466, 1209, 1896
BssMI GATC 7 cut(s) 60, 220, 310, 735, 991, 1320, 1477
BssSI CACGAG 1 cut(s) 1216
BssT1I CCWWGG 2 cut(s) 466, 1209
Bst2BI CACGAG 1 cut(s) 1216
Bst2UI CCWGG 2 cut(s) 299, 484
Bst4CI ACNGT 2 cut(s) 20, 1293
Bst6I CTCTTC 3 cut(s) 1053, 1697, 1885
BstAUI TGTACA 1 cut(s) 1750
BstBAI YACGTR 2 cut(s) 110, 1394
BstC8I GCNNGC 6 cut(s) 906, 948, 1136, 1686, 1848, 1859
BstDEI CTNAG 5 cut(s) 103, 132, 747, 1008, 1604
BstDSI CCRYGG 1 cut(s) 1896
BstF5I GGATG 4 cut(s) 172, 1061, 1181, 1978
BstFNI CGCG 1 cut(s) 1898
BstHHI GCGC 1 cut(s) 1684
BstKTI GATC 7 cut(s) 63, 223, 313, 738, 994, 1323, 1480
BstMBI GATC 7 cut(s) 60, 220, 310, 735, 991, 1320, 1477
BstMCI CGRYCG 1 cut(s) 1512
BstMWI GCNNNNNNNGC 3 cut(s) 248, 884, 943
BstNI CCWGG 2 cut(s) 299, 484
BstNSI RCATGY 2 cut(s) 1287, 1332
BstSCI CCNGG 4 cut(s) 297, 482, 688, 756
BstSLI GKGCMC 4 cut(s) 78, 794, 910, 1217
BstUI CGCG 1 cut(s) 1898
BstV1I GCAGC 3 cut(s) 1216, 1477, 1957
BstV2I GAAGAC 2 cut(s) 1328, 1729
BstX2I RGATCY 4 cut(s) 60, 220, 735, 1477
BstXI CCANNNNNNTGG 2 cut(s) 1331, 1569
BstYI RGATCY 4 cut(s) 60, 220, 735, 1477
Bsu36I CCTNAGG 1 cut(s) 132
BsuI GTATCC 1 cut(s) 507
BsuRI GGCC 6 cut(s) 207, 464, 681, 966, 1134, 1609
BtgI CCRYGG 1 cut(s) 1896
BtsCI GGATG 4 cut(s) 172, 1061, 1181, 1978
BtsI GCAGTG 1 cut(s) 1677
BtsIMutI CAGTG 4 cut(s) 418, 1419, 1617, 1677
BveI ACCTGC 2 cut(s) 805, 1212
Cac8I GCNNGC 6 cut(s) 906, 948, 1136, 1686, 1848, 1859
CfoI GCGC 1 cut(s) 1684
Cfr13I GGNCC 7 cut(s) 264, 407, 434, 463, 1581, 1655, 1838
Cfr42I CCGCGG 1 cut(s) 1899
CseI GACGC 1 cut(s) 1898
Csp6I GTAC 1 cut(s) 1751
CviQI GTAC 1 cut(s) 1751
DdeI CTNAG 5 cut(s) 103, 132, 747, 1008, 1604
DpnI GATC 7 cut(s) 62, 222, 312, 737, 993, 1322, 1479
DpnII GATC 7 cut(s) 60, 220, 310, 735, 991, 1320, 1477
DraI TTTAAA 1 cut(s) 891
DraIII CACNNNGTG 1 cut(s) 789
DrdI GACNNNNNNGTC 1 cut(s) 1457
DriI GACNNNNNGTC 1 cut(s) 725
DseDI GACNNNNNNGTC 1 cut(s) 1457
EaeI YGGCCR 3 cut(s) 205, 679, 964
Eam1104I CTCTTC 3 cut(s) 1053, 1697, 1885
Eam1105I GACNNNNNGTC 1 cut(s) 725
EarI CTCTTC 3 cut(s) 1053, 1697, 1885
EciI GGCGGA 1 cut(s) 1209
Ecl136II GAGCTC 1 cut(s) 1145
Eco130I CCWWGG 2 cut(s) 466, 1209
Eco24I GRGCYC 1 cut(s) 1147
Eco47I GGWCC 6 cut(s) 264, 407, 434, 1581, 1655, 1838
Eco53kI GAGCTC 1 cut(s) 1145
Eco57I CTGAAG 4 cut(s) 182, 1218, 1602, 1678
Eco72I CACGTG 1 cut(s) 110
Eco81I CCTNAGG 1 cut(s) 132
EcoICRI GAGCTC 1 cut(s) 1145
EcoO109I RGGNCCY 5 cut(s) 407, 463, 1581, 1655, 1838
EcoRI GAATTC 3 cut(s) 597, 1954, 1996
EcoRII CCWGG 2 cut(s) 297, 482
EcoT14I CCWWGG 2 cut(s) 466, 1209
EcoT22I ATGCAT 1 cut(s) 1255
EcoT38I GRGCYC 1 cut(s) 1147
ErhI CCWWGG 2 cut(s) 466, 1209
FaqI GGGAC 4 cut(s) 393, 840, 1594, 1641
FauNDI CATATG 2 cut(s) 765, 1368
FblI GTMKAC 2 cut(s) 352, 1449
Fnu4HI GCNGC 4 cut(s) 1230, 1491, 1896, 1971
FokI GGATG 4 cut(s) 179, 1048, 1168, 1965
FriOI GRGCYC 1 cut(s) 1147
Fsp4HI GCNGC 4 cut(s) 1230, 1491, 1896, 1971
FspBI CTAG 5 cut(s) 248, 830, 1382, 1574, 1673
FspI TGCGCA 1 cut(s) 1683
GlaI GCGC 1 cut(s) 1683
GluI GCNGC 4 cut(s) 1230, 1491, 1896, 1971
GsuI CTGGAG 1 cut(s) 894
HaeIII GGCC 6 cut(s) 207, 464, 681, 966, 1134, 1609
HapII CCGG 4 cut(s) 690, 758, 1645, 1913
HgaI GACGC 1 cut(s) 1898
HhaI GCGC 1 cut(s) 1684
Hin6I GCGC 1 cut(s) 1682
HinP1I GCGC 1 cut(s) 1682
HincII GTYRAC 1 cut(s) 730
HindII GTYRAC 1 cut(s) 730
HindIII AAGCTT 1 cut(s) 234
HinfI GANTC 8 cut(s) 400, 472, 584, 720, 749, 922, 1205, 1451
HpaII CCGG 4 cut(s) 690, 758, 1645, 1913
HphI GGTGA 1 cut(s) 394
Hpy166II GTNNAC 5 cut(s) 100, 353, 730, 1450, 1819
Hpy188I TCNGA 4 cut(s) 707, 748, 961, 1089
Hpy8I GTNNAC 5 cut(s) 100, 353, 730, 1450, 1819
Hpy99I CGWCG 2 cut(s) 937, 2005
HpyAV CCTTC 5 cut(s) 481, 1197, 1571, 1820, 1937
HpyCH4III ACNGT 2 cut(s) 20, 1293
HpyCH4IV ACGT 3 cut(s) 109, 932, 1393
HpyF10VI GCNNNNNNNGC 3 cut(s) 248, 884, 943
HpyF3I CTNAG 5 cut(s) 103, 132, 747, 1008, 1604
HpySE526I ACGT 3 cut(s) 109, 932, 1393
HspAI GCGC 1 cut(s) 1682
KflI GGGWCCC 2 cut(s) 1581, 1655
Kpn2I TCCGGA 2 cut(s) 1644, 1912
KspI CCGCGG 1 cut(s) 1899
Kzo9I GATC 7 cut(s) 60, 220, 310, 735, 991, 1320, 1477
LguI GCTCTTC 1 cut(s) 1885
LmnI GCTCC 7 cut(s) 282, 300, 537, 875, 1142, 1150, 1668
Lsp1109I GCAGC 3 cut(s) 1216, 1477, 1957
LweI GCATC 5 cut(s) 57, 565, 874, 1262, 1397
MaeI CTAG 5 cut(s) 248, 830, 1382, 1574, 1673
MaeII ACGT 3 cut(s) 109, 932, 1393
MaeIII GTNAC 6 cut(s) 14, 139, 314, 656, 1553, 1612
MalI GATC 7 cut(s) 62, 222, 312, 737, 993, 1322, 1479
MboI GATC 7 cut(s) 60, 220, 310, 735, 991, 1320, 1477
MflI RGATCY 4 cut(s) 60, 220, 735, 1477
MhlI GDGCHC 5 cut(s) 78, 794, 910, 1147, 1217
MlsI TGGCCA 3 cut(s) 207, 681, 966
MluNI TGGCCA 3 cut(s) 207, 681, 966
MlyI GAGTC 4 cut(s) 714, 758, 916, 1445
MmeI TCCRAC 4 cut(s) 171, 953, 1455, 1505
Mox20I TGGCCA 3 cut(s) 207, 681, 966
Mph1103I ATGCAT 1 cut(s) 1255
MroI TCCGGA 2 cut(s) 1644, 1912
MroXI GAANNNNTTC 1 cut(s) 597
MscI TGGCCA 3 cut(s) 207, 681, 966
MseI TTAA 8 cut(s) 90, 441, 624, 638, 890, 1115, 1718, 1940
MslI CAYNNNNRTG 1 cut(s) 1567
Msp20I TGGCCA 3 cut(s) 207, 681, 966
MspA1I CMGCKG 4 cut(s) 590, 1012, 1229, 1898
MspI CCGG 4 cut(s) 690, 758, 1645, 1913
MspR9I CCNGG 4 cut(s) 299, 484, 690, 758
Mva1269I GAATGC 2 cut(s) 974, 1640
MvaI CCWGG 2 cut(s) 299, 484
MvnI CGCG 1 cut(s) 1898
MwoI GCNNNNNNNGC 3 cut(s) 248, 884, 943
NciI CCSGG 2 cut(s) 690, 758
NdeI CATATG 2 cut(s) 765, 1368
NdeII GATC 7 cut(s) 60, 220, 310, 735, 991, 1320, 1477
NmuCI GTSAC 4 cut(s) 14, 314, 656, 1612
NsbI TGCGCA 1 cut(s) 1683
NsiI ATGCAT 1 cut(s) 1255
NspI RCATGY 2 cut(s) 1287, 1332
OliI CACNNNNGTG 1 cut(s) 1567
PaqCI CACCTGC 1 cut(s) 805
PciSI GCTCTTC 1 cut(s) 1885
PctI GAATGC 2 cut(s) 974, 1640
PdmI GAANNNNTTC 1 cut(s) 597
PfeI GAWTC 4 cut(s) 400, 472, 584, 1205
PfoI TCCNGGA 2 cut(s) 482, 688
PkrI GCNGC 4 cut(s) 1231, 1492, 1897, 1972
PleI GAGTC 4 cut(s) 714, 757, 916, 1445
PmaCI CACGTG 1 cut(s) 110
PmlI CACGTG 1 cut(s) 110
PpsI GAGTC 4 cut(s) 714, 757, 916, 1445
Ppu21I YACGTR 2 cut(s) 110, 1394
PpuMI RGGWCCY 4 cut(s) 407, 1581, 1655, 1838
PshBI ATTAAT 1 cut(s) 1115
Psp124BI GAGCTC 1 cut(s) 1147
Psp5II RGGWCCY 4 cut(s) 407, 1581, 1655, 1838
Psp6I CCWGG 2 cut(s) 297, 482
PspCI CACGTG 1 cut(s) 110
PspGI CCWGG 2 cut(s) 297, 482
PspPI GGNCC 7 cut(s) 264, 407, 434, 463, 1581, 1655, 1838
PspPPI RGGWCCY 4 cut(s) 407, 1581, 1655, 1838
PsuI RGATCY 4 cut(s) 60, 220, 735, 1477
PvuII CAGCTG 3 cut(s) 590, 1012, 1229
RsaI GTAC 1 cut(s) 1752
RsaNI GTAC 1 cut(s) 1751
RseI CAYNNNNRTG 1 cut(s) 1567
SacI GAGCTC 1 cut(s) 1147
SacII CCGCGG 1 cut(s) 1899
SapI GCTCTTC 1 cut(s) 1885
SaqAI TTAA 8 cut(s) 90, 441, 624, 638, 890, 1115, 1718, 1940
SatI GCNGC 4 cut(s) 1230, 1491, 1896, 1971
Sau3AI GATC 7 cut(s) 60, 220, 310, 735, 991, 1320, 1477
Sau96I GGNCC 7 cut(s) 264, 407, 434, 463, 1581, 1655, 1838
SchI GAGTC 4 cut(s) 714, 758, 916, 1445
ScrFI CCNGG 4 cut(s) 299, 484, 690, 758
SduI GDGCHC 5 cut(s) 78, 794, 910, 1147, 1217
SfaNI GCATC 5 cut(s) 57, 565, 874, 1262, 1397
Sfr303I CCGCGG 1 cut(s) 1899
SgrBI CCGCGG 1 cut(s) 1899
SinI GGWCC 6 cut(s) 264, 407, 434, 1581, 1655, 1838
SmiMI CAYNNNNRTG 1 cut(s) 1567
SmlI CTYRAG 2 cut(s) 115, 1494
SmoI CTYRAG 2 cut(s) 115, 1494
SpeI ACTAGT 1 cut(s) 829
SsiI CCGC 4 cut(s) 1194, 1512, 1896, 1898
SspI AATATT 3 cut(s) 151, 711, 1525
SspMI CTAG 5 cut(s) 248, 830, 1382, 1574, 1673
SstI GAGCTC 1 cut(s) 1147
StyD4I CCNGG 4 cut(s) 297, 482, 688, 756
StyI CCWWGG 2 cut(s) 466, 1209
TaaI ACNGT 2 cut(s) 20, 1293
TaiI ACGT 3 cut(s) 112, 935, 1396
TaqI TCGA 7 cut(s) 357, 403, 510, 723, 994, 1270, 1508
TatI WGTACW 1 cut(s) 1750
TauI GCSGC 1 cut(s) 1898
TfiI GAWTC 4 cut(s) 400, 472, 584, 1205
Tru1I TTAA 8 cut(s) 90, 441, 624, 638, 890, 1115, 1718, 1940
Tru9I TTAA 8 cut(s) 90, 441, 624, 638, 890, 1115, 1718, 1940
TscAI CASTG 4 cut(s) 425, 1426, 1617, 1684
TseFI GTSAC 4 cut(s) 14, 314, 656, 1612
TseI GCWGC 3 cut(s) 1229, 1490, 1970
Tsp45I GTSAC 4 cut(s) 14, 314, 656, 1612
TspDTI ATGAA 6 cut(s) 167, 782, 1050, 1355, 1529, 1839
TspGWI ACGGA 2 cut(s) 534, 1155
TspRI CASTG 4 cut(s) 425, 1426, 1617, 1684
VpaK11BI GGWCC 6 cut(s) 264, 407, 434, 1581, 1655, 1838
VspI ATTAAT 1 cut(s) 1115
XapI RAATTY 7 cut(s) 183, 597, 769, 778, 984, 1954, 1996
XbaI TCTAGA 1 cut(s) 1381
XceI RCATGY 2 cut(s) 1287, 1332
XmiI GTMKAC 2 cut(s) 352, 1449
XmnI GAANNNNTTC 1 cut(s) 597
XspI CTAG 5 cut(s) 248, 830, 1382, 1574, 1673
Zsp2I ATGCAT 1 cut(s) 1255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.