pycom13g02340

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005436
Physical Location & Seq
Forward (+)
1608817 .. 1616137
7321 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2889 bp
ATGGCACCCACAGTCTCAGCTGTGCAACCTTGCTTCAGCGGGAATGGATTGAGAAGTGCCATAGCTTTCAAAGCTGAGAAGGGATATAGGAATGCTTTGGGTAGAAAAATGAGGTTCAGTAATTTTCTCTTTTCAACGGTATCAAGATTATGTTTTCGTCCAAAGCATAGTCTTGTCAAAAATAATTTGGAGGAAGTAGATATTTTTGGCATAGCAAGCATCTCAGATCGGTCAAAGTTGCTCAATAAGGTATCTGCTTTGGTTGGTTATGATAGTCTTGATGATTTGATTGAGAATGAAAGAGCTGAAAAGCAGTCCGGTATGCATGTTAGAGATGTCATAGATGAATTTGATGTCTCCTTGGTGTGTGCACGATTTCCATCAATCATTTTAGGTAGTTCCCCTCGGGTGGAATTGTATGATGGAACCACCAGTTTCAGTGAAAGGATGCTTTTGTCAACTCAAAGTGGTGAAGGATTTCTGTCTGATACTATGTGTGAAGAGCAGTCCGCAACTGTGGTTATTGAGGAAAACTCACATCAAACAGTTCCTGTGGAGGAATCTTCTAACAAGGTTTTGCTTAAATCACAAAATGATACTGGGTCTGTTCAGTTTTCCCTGGACAGTTCTATCAGTTGCATACCTGGAATAACTAAGAAGCGCTGTCGGCAGCTAGAAGACTGTGGCTTTCATACATTGCGGCAATTGCTTCACCACTTTCCTCGGACCTATGCGGATTTACAGAATGCTCAGATGAAAATTGATGATGGACAATACTTGATTTTCATTGGGAAGGTATTAAGTTCGAGGGGAATCAAAGCTAGTAGTTCTTTCTCATTTATTGAGGTAGTTGTTGGTTGCGAAATTATGGACGATGAATCAACTGAGCATTTGAATGATTCTGCTGATAGTAGAAGAAAGAAGACAATTTCTTTGCATCTGAAGAAATTTTTTCGTGGTGCTCGTTTTACTTCTCTACCCTTTCTTAGACTTGTTGAGGGAAAGCATAAAGAGGGGGACTTTGTATGTGTTAGTGGCAAGGTAAGGACAATGCCTGCCAAAGGTCACTATGAAATGAGAGAATACAATATTGATGTGCTTAAAGATGAAAACGAAGTGTCTTTTCATGCAAAAGGGAGGCCTTATCCTATATACCCTTCAAAAGGAGGTATCGATCCAAATGTTTTGAGAGACATCATTGCAAGAGTTGCACAAGTTTTGGCTGTCGATGTTGATCCTATTCCTAAAAATATCACTCAGGACTTTGGGCTGTTAGGCCTTCATGATGCGTATAAGGGGATTCACGAACCAAAGAGTATGGAAGAAGCTGATTTGGCTCGGAAAAGGCTAATATTTGATGAGTTCTTTTACCTGCAGTTAGCGCGCCTTTACCAAATGCTTGAAGGTCTTGGTACACAAATAGAGAAAGATGGATTGCTGGATAAGTACAGGAAACCTGAATCAAGTGCTGCTTATATGAAGGAATGGTCCATTCTTACCAAGAAGTTTTCAAAAGCTCTTCCATATACCCTTACTCCTAGTCAACTCACTGCCGTTTCAGAAATCATCTGGGATCTAAGGCAACCAGTTCCTATGAATCGGCTTTTGCAGGGTGATGTTGGTTGTGGGAAAACCGTGGTAGCTTTTCTAGCATGCATGGAGGTTATTGGCTCTGGATATCAGGCAGCTTTCATGGTTCCAACTGAGTTGCTTGCAGTCCAGCATTACGAGCACTTAAATAAATTGCTTGAAAATATGGAGGATGTTGAACGAAAACCTTCAATTGCTTTACTAACAGGCTCAACCCCATCAAAACAATCACGGATAATTCTTGAGGGTCTCAGGACTGGAGAAATATCAATGGTCGTTGGAACCACCAGTTTAATATCTGACAAAATCGAATTCTTTGCATTACGCATTGCAGTGGTGGATGAACAACACCGTTTTGGTGTGATCCAGAGAGGACGGTTTAACAGTAAGCTATACTATACCTCCATAAATTCTAGAATGGTAGCCGCCAGTTCAGATGTGACCTCAAAAGGTGACAACCATATGGCTCCTCATGTTCTTGCCATGTCAGCTACTCCTATCCCGAGAACACTTGCTCTTGCTTTATATGGGGATATGTCATTGACACAGATAACGGACTTACCTCCAGGAAGAATACCAGTTGAGACATTCATCATTGAAGGAAATGACAATGGATTTGAGGATGTTTATGAGATGATGCTGGACGAGTTAAAAGAAGGAGGGAGAGTCTATCTTGTGTATCCAGTTATTGAACAATCTGAACAGCTGCCACAACTTCGTGCTGCTGCGGCAGATTTTGAAGCTATATCTGATAGGTTTCAGGGCTACACTTGTGGATTGTTGCATGGAAGGATGAAGAGTGATGAGAAAGATGAAGCGTTGAGAAAGTTCAGATCTGGAGAAACAGATATACTGCTTTCCACACAAGTAATTGAGATTGGTGTTGATGTTCCGGATGCATCTATGATGGTTGTTATGAATGCTGAGAGATTTGGGATAGCTCAGTTACACCAACTTAGAGGACGAGTTGGACGAGGAGTGAGAAAGTCTAAATGTATACTTTTAGCATCTTCTTCCAGTAGTCTAACTCGGCTGATGGTCCTGGGGAAATCGTCAGATGGTTTTTATTTGGCAAACATGGACCTTCTTTTACGTGGACCTGGTAACTTGCTTGGCAAGAAACAGTCAGGACATCTTCCAGAGTTTCCTATTGCAAGATTGGAAGTAGATGGCAATATCTTACAAGAAGCGCATCTTGCTGCTCTGAAAGTGTTAGGTGCTTCTAATGATTTGGAGCAATTCCCGCTGCTTAAGGCAGAGCTTAGCATGAGACAGCCGCTGTCTATTCTGGGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

963

Amino Acids

107.05

Weight (kDa)

6.24

Isoelectric Point (pI)

41.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 510 - 696 4.1e-08 Type III restriction enzyme, res subunit
DEAD PF00270 514 - 700 6.1e-18 DEAD/DEAH box helicase
Helicase_C PF00271 741 - 855 1.7e-19 Helicase conserved C-terminal domain
RecG_dom3_C PF19833 882 - 939 4e-08 ATP-dependent DNA helicase RecG, domain 3, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1380
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 2587
AccII CGCG 1 cut(s) 1384
AccIII TCCGGA 1 cut(s) 2482
AciI CCGC 8 cut(s) 39, 510, 700, 734, 2016, 2318, 2834, 2867
AclWI GGATC 4 cut(s) 1169, 1229, 1581, 1948
AcsI RAATTY 4 cut(s) 347, 947, 1901, 1999
AcuI CTGAAG 2 cut(s) 19, 962
AfaI GTAC 2 cut(s) 1414, 1448
AfeI AGCGCT 1 cut(s) 662
AfiI CCNNNNNNNGG 3 cut(s) 409, 1061, 1163
AflII CTTAAG 1 cut(s) 2840
AjnI CCWGG 5 cut(s) 618, 643, 2155, 2631, 2689
Alw21I GWGCWC 3 cut(s) 373, 964, 1734
Alw26I GTCTC 6 cut(s) 19, 361, 1185, 1844, 2168, 2854
Alw44I GTGCAC 1 cut(s) 369
AlwI GGATC 4 cut(s) 1169, 1229, 1581, 1948
AlwNI CAGNNNCTG 2 cut(s) 551, 2869
Ama87I CYCGRG 2 cut(s) 405, 2092
Aor13HI TCCGGA 1 cut(s) 2482
Aor51HI AGCGCT 1 cut(s) 662
AoxI GGCC 2 cut(s) 1139, 1276
ApaLI GTGCAC 1 cut(s) 369
ApeKI GCWGC 8 cut(s) 670, 1469, 1685, 2296, 2312, 2315, 2789, 2836
ApoI RAATTY 4 cut(s) 347, 947, 1901, 1999
ArsI GACNNNNNNTTYG 4 cut(s) 916, 948, 2189, 2221
Asp700I GAANNNNTTC 2 cut(s) 477, 1777
AspLEI GCGC 4 cut(s) 663, 1384, 1386, 2782
AspS9I GGNCC 5 cut(s) 726, 1488, 2629, 2671, 2687
AsuHPI GGTGA 4 cut(s) 482, 704, 1625, 2054
AvaI CYCGRG 2 cut(s) 405, 2092
AvaII GGWCC 5 cut(s) 726, 1488, 2629, 2671, 2687
BaeGI GKGCMC 1 cut(s) 373
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 2 cut(s) 684, 929
Bbv12I GWGCWC 3 cut(s) 373, 964, 1734
BbvI GCAGC 8 cut(s) 682, 1456, 1697, 2283, 2299, 2302, 2776, 2823
BccI CCATC 9 cut(s) 388, 416, 761, 1424, 1816, 2491, 2620, 2642, 2753
BceAI ACGGC 1 cut(s) 1538
BcgI CGANNNNNNTGC 2 cut(s) 1889, 1923
BciT130I CCWGG 5 cut(s) 620, 645, 2157, 2633, 2691
BciVI GTATCC 1 cut(s) 2280
BcoDI GTCTC 6 cut(s) 19, 361, 1185, 1844, 2168, 2854
BfaI CTAG 5 cut(s) 674, 822, 1539, 1649, 2004
BfmI CTRYAG 1 cut(s) 1373
BfoI RGCGCY 1 cut(s) 664
BfrI CTTAAG 1 cut(s) 2840
BfuAI ACCTGC 1 cut(s) 1380
BfuI GTATCC 1 cut(s) 2280
BglII AGATCT 1 cut(s) 2423
BlpI GCTNAGC 1 cut(s) 2852
Bme1390I CCNGG 5 cut(s) 620, 645, 2157, 2633, 2691
Bme18I GGWCC 5 cut(s) 726, 1488, 2629, 2671, 2687
BmeT110I CYCGRG 2 cut(s) 405, 2092
BmgT120I GGNCC 5 cut(s) 726, 1488, 2629, 2671, 2687
BmiI GGNNCC 5 cut(s) 6, 427, 1698, 1873, 2058
BmrFI CCNGG 5 cut(s) 620, 645, 2157, 2633, 2691
BmrI ACTGGG 1 cut(s) 609
BmsI GCATC 9 cut(s) 228, 438, 946, 1276, 2217, 2476, 2498, 2606, 2791
BmuI ACTGGG 1 cut(s) 609
BpiI GAAGAC 2 cut(s) 684, 929
BplI GAGNNNNNCTC 2 cut(s) 518, 550
BpmI CTGGAG 3 cut(s) 1869, 2139, 2448
Bpu1102I GCTNAGC 1 cut(s) 2852
BpuEI CTTGAG 1 cut(s) 1853
Bsa29I ATCGAT 1 cut(s) 1173
BsaAI YACGTR 1 cut(s) 2684
BsaBI GATNNNNATC 2 cut(s) 379, 1233
BsaI GGTCTC 1 cut(s) 1844
BsaJI CCNNGG 6 cut(s) 360, 404, 618, 722, 1635, 2632
BsaWI WCCGGW 2 cut(s) 317, 2482
BsaXI ACNNNNNCTCC 6 cut(s) 1519, 1549, 1976, 2006, 2241, 2271
Bsc4I CCNNNNNNNGG 3 cut(s) 409, 1061, 1163
Bse1I ACTGG 9 cut(s) 432, 604, 1586, 1852, 1878, 2019, 2168, 2273, 2607
Bse3DI GCAATG 3 cut(s) 695, 1197, 1917
Bse8I GATNNNNATC 2 cut(s) 379, 1233
BseAI TCCGGA 1 cut(s) 2482
BseBI CCWGG 5 cut(s) 620, 645, 2157, 2633, 2691
BseCI ATCGAT 1 cut(s) 1173
BseDI CCNNGG 6 cut(s) 360, 404, 618, 722, 1635, 2632
BseGI GGATG 6 cut(s) 453, 1768, 1936, 2218, 2388, 2491
BseJI GATNNNNATC 2 cut(s) 379, 1233
BseLI CCNNNNNNNGG 3 cut(s) 409, 1061, 1163
BseMI GCAATG 3 cut(s) 695, 1197, 1917
BseNI ACTGG 9 cut(s) 432, 604, 1586, 1852, 1878, 2019, 2168, 2273, 2607
BsePI GCGCGC 1 cut(s) 1382
BseRI GAGGAG 2 cut(s) 2049, 2580
BseSI GKGCMC 1 cut(s) 373
BseXI GCAGC 8 cut(s) 682, 1456, 1697, 2283, 2299, 2302, 2776, 2823
Bsh1236I CGCG 1 cut(s) 1384
BshFI GGCC 2 cut(s) 1141, 1278
BshNI GGYRCC 1 cut(s) 4
BshVI ATCGAT 1 cut(s) 1173
BsiHKAI GWGCWC 3 cut(s) 373, 964, 1734
BsiHKCI CYCGRG 2 cut(s) 405, 2092
BsiSI CCGG 2 cut(s) 318, 2483
BslFI GGGAC 1 cut(s) 1031
BslI CCNNNNNNNGG 3 cut(s) 409, 1061, 1163
BsmAI GTCTC 6 cut(s) 19, 361, 1185, 1844, 2168, 2854
BsmFI GGGAC 1 cut(s) 1031
BsmI GAATGC 3 cut(s) 97, 751, 2515
BsnI GGCC 2 cut(s) 1141, 1278
Bso31I GGTCTC 1 cut(s) 1844
BsoBI CYCGRG 2 cut(s) 405, 2092
Bsp1286I GDGCHC 3 cut(s) 373, 964, 1734
Bsp13I TCCGGA 1 cut(s) 2482
Bsp143I GATC 6 cut(s) 226, 1174, 1234, 1573, 1953, 2423
Bsp1720I GCTNAGC 1 cut(s) 2852
BspACI CCGC 8 cut(s) 39, 510, 700, 734, 2016, 2318, 2834, 2867
BspANI GGCC 2 cut(s) 1141, 1278
BspDI ATCGAT 1 cut(s) 1173
BspEI TCCGGA 1 cut(s) 2482
BspFNI CGCG 1 cut(s) 1384
BspHI TCATGA 1 cut(s) 1282
BspLI GGNNCC 5 cut(s) 6, 427, 1698, 1873, 2058
BspMAI CTGCAG 1 cut(s) 1377
BspMI ACCTGC 1 cut(s) 1380
BspPI GGATC 4 cut(s) 1169, 1229, 1581, 1948
BspQI GCTCTTC 2 cut(s) 495, 1524
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 2840
BspTNI GGTCTC 1 cut(s) 1844
BsrDI GCAATG 3 cut(s) 695, 1197, 1917
BsrI ACTGG 9 cut(s) 432, 604, 1586, 1852, 1878, 2019, 2168, 2273, 2607
BssECI CCNNGG 6 cut(s) 360, 404, 618, 722, 1635, 2632
BssHII GCGCGC 1 cut(s) 1382
BssMI GATC 6 cut(s) 226, 1174, 1234, 1573, 1953, 2423
BssNAI GTATAC 1 cut(s) 2588
BssT1I CCWWGG 1 cut(s) 360
Bst1107I GTATAC 1 cut(s) 2588
Bst2UI CCWGG 5 cut(s) 620, 645, 2157, 2633, 2691
Bst6I CTCTTC 3 cut(s) 495, 1524, 2381
BstAFI CTTAAG 1 cut(s) 2840
BstAPI GCANNNNNTGC 1 cut(s) 1208
BstBAI YACGTR 1 cut(s) 2684
BstC8I GCNNGC 5 cut(s) 217, 1056, 1384, 1654, 1713
BstDSI CCRYGG 1 cut(s) 1635
BstENI CCTNNNNNAGG 2 cut(s) 1059, 1161
BstF5I GGATG 6 cut(s) 453, 1768, 1936, 2218, 2388, 2491
BstFNI CGCG 1 cut(s) 1384
BstH2I RGCGCY 1 cut(s) 664
BstHHI GCGC 4 cut(s) 663, 1384, 1386, 2782
BstKTI GATC 6 cut(s) 229, 1177, 1237, 1576, 1956, 2426
BstMAI GTCTC 6 cut(s) 19, 361, 1185, 1844, 2168, 2854
BstMBI GATC 6 cut(s) 226, 1174, 1234, 1573, 1953, 2423
BstNI CCWGG 5 cut(s) 620, 645, 2157, 2633, 2691
BstNSI RCATGY 2 cut(s) 329, 1656
BstSCI CCNGG 5 cut(s) 618, 643, 2155, 2631, 2689
BstSFI CTRYAG 1 cut(s) 1373
BstSLI GKGCMC 1 cut(s) 373
BstUI CGCG 1 cut(s) 1384
BstV1I GCAGC 8 cut(s) 682, 1456, 1697, 2283, 2299, 2302, 2776, 2823
BstV2I GAAGAC 2 cut(s) 684, 929
BstX2I RGATCY 2 cut(s) 1573, 2423
BstYI RGATCY 2 cut(s) 1573, 2423
BstZ17I GTATAC 1 cut(s) 2588
Bsu15I ATCGAT 1 cut(s) 1173
BsuI GTATCC 1 cut(s) 2280
BsuRI GGCC 2 cut(s) 1141, 1278
BsuTUI ATCGAT 1 cut(s) 1173
BtgI CCRYGG 1 cut(s) 1635
BtsCI GGATG 6 cut(s) 453, 1768, 1936, 2218, 2388, 2491
BtsI GCAGTG 2 cut(s) 1548, 1929
BtsIMutI CAGTG 3 cut(s) 445, 1548, 1929
BveI ACCTGC 1 cut(s) 1380
Cac8I GCNNGC 5 cut(s) 217, 1056, 1384, 1654, 1713
CaiI CAGNNNCTG 2 cut(s) 551, 2869
CciI TCATGA 1 cut(s) 1282
CfoI GCGC 4 cut(s) 663, 1384, 1386, 2782
Cfr13I GGNCC 5 cut(s) 726, 1488, 2629, 2671, 2687
ClaI ATCGAT 1 cut(s) 1173
CsiI ACCWGGT 1 cut(s) 2689
Csp6I GTAC 2 cut(s) 1413, 1447
CviQI GTAC 2 cut(s) 1413, 1447
DpnI GATC 6 cut(s) 228, 1176, 1236, 1575, 1955, 2425
DpnII GATC 6 cut(s) 226, 1174, 1234, 1573, 1953, 2423
Eam1104I CTCTTC 3 cut(s) 495, 1524, 2381
EarI CTCTTC 3 cut(s) 495, 1524, 2381
Eco130I CCWWGG 1 cut(s) 360
Eco147I AGGCCT 2 cut(s) 1141, 1278
Eco31I GGTCTC 1 cut(s) 1844
Eco32I GATATC 1 cut(s) 1679
Eco47I GGWCC 5 cut(s) 726, 1488, 2629, 2671, 2687
Eco47III AGCGCT 1 cut(s) 662
Eco57I CTGAAG 2 cut(s) 19, 962
Eco88I CYCGRG 2 cut(s) 405, 2092
EcoNI CCTNNNNNAGG 2 cut(s) 1059, 1161
EcoRI GAATTC 1 cut(s) 1901
EcoRII CCWGG 5 cut(s) 618, 643, 2155, 2631, 2689
EcoRV GATATC 1 cut(s) 1679
EcoT14I CCWWGG 1 cut(s) 360
EcoT22I ATGCAT 3 cut(s) 327, 1658, 2491
ErhI CCWWGG 1 cut(s) 360
FalI AAGNNNNNCTT 4 cut(s) 1456, 1488, 2769, 2801
FaqI GGGAC 1 cut(s) 1031
FauI CCCGC 2 cut(s) 32, 2841
FauNDI CATATG 1 cut(s) 2052
FblI GTMKAC 1 cut(s) 2587
FokI GGATG 6 cut(s) 460, 1775, 1943, 2225, 2395, 2498
FspBI CTAG 5 cut(s) 674, 822, 1539, 1649, 2004
GlaI GCGC 4 cut(s) 662, 1383, 1385, 2781
GsuI CTGGAG 3 cut(s) 1869, 2139, 2448
HaeII RGCGCY 1 cut(s) 664
HaeIII GGCC 2 cut(s) 1141, 1278
HapII CCGG 2 cut(s) 318, 2483
HhaI GCGC 4 cut(s) 663, 1384, 1386, 2782
Hin6I GCGC 4 cut(s) 661, 1382, 1384, 2780
HinP1I GCGC 4 cut(s) 661, 1382, 1384, 2780
HincII GTYRAC 2 cut(s) 459, 1544
HindII GTYRAC 2 cut(s) 459, 1544
HinfI GANTC 8 cut(s) 560, 813, 878, 899, 1300, 1460, 1597, 2256
HpaII CCGG 2 cut(s) 318, 2483
HphI GGTGA 4 cut(s) 482, 704, 1625, 2054
Hpy166II GTNNAC 6 cut(s) 371, 459, 1415, 1544, 2588, 2687
Hpy8I GTNNAC 6 cut(s) 371, 459, 1415, 1544, 2588, 2687
HpyCH4IV ACGT 1 cut(s) 2683
HpySE526I ACGT 1 cut(s) 2683
HspAI GCGC 4 cut(s) 661, 1382, 1384, 2780
Kpn2I TCCGGA 1 cut(s) 2482
Kzo9I GATC 6 cut(s) 226, 1174, 1234, 1573, 1953, 2423
LguI GCTCTTC 2 cut(s) 495, 1524
LmnI GCTCC 2 cut(s) 2062, 2824
Lsp1109I GCAGC 8 cut(s) 682, 1456, 1697, 2283, 2299, 2302, 2776, 2823
LweI GCATC 9 cut(s) 228, 438, 946, 1276, 2217, 2476, 2498, 2606, 2791
MabI ACCWGGT 1 cut(s) 2689
MaeI CTAG 5 cut(s) 674, 822, 1539, 1649, 2004
MaeII ACGT 1 cut(s) 2683
MaeIII GTNAC 6 cut(s) 1064, 2029, 2042, 2535, 2693, 2882
MalI GATC 6 cut(s) 228, 1176, 1236, 1575, 1955, 2425
MboI GATC 6 cut(s) 226, 1174, 1234, 1573, 1953, 2423
MfeI CAATTG 2 cut(s) 704, 1782
MflI RGATCY 2 cut(s) 1573, 2423
MhlI GDGCHC 3 cut(s) 373, 964, 1734
MlyI GAGTC 1 cut(s) 2265
MmeI TCCRAC 3 cut(s) 1724, 1849, 2539
Mph1103I ATGCAT 3 cut(s) 327, 1658, 2491
MroI TCCGGA 1 cut(s) 2482
MroXI GAANNNNTTC 2 cut(s) 477, 1777
MseI TTAA 8 cut(s) 582, 800, 1101, 1736, 1883, 1971, 2240, 2841
MslI CAYNNNNRTG 2 cut(s) 894, 1922
MspA1I CMGCKG 5 cut(s) 20, 39, 2296, 2836, 2869
MspCI CTTAAG 1 cut(s) 2840
MspI CCGG 2 cut(s) 318, 2483
MspR9I CCNGG 5 cut(s) 620, 645, 2157, 2633, 2691
MunI CAATTG 2 cut(s) 704, 1782
Mva1269I GAATGC 3 cut(s) 97, 751, 2515
MvaI CCWGG 5 cut(s) 620, 645, 2157, 2633, 2691
MvnI CGCG 1 cut(s) 1384
NdeI CATATG 1 cut(s) 2052
NdeII GATC 6 cut(s) 226, 1174, 1234, 1573, 1953, 2423
NlaIV GGNNCC 5 cut(s) 6, 427, 1698, 1873, 2058
NmeAIII GCCGAG 1 cut(s) 2599
NmuCI GTSAC 4 cut(s) 1064, 2029, 2042, 2882
NsiI ATGCAT 3 cut(s) 327, 1658, 2491
NspI RCATGY 2 cut(s) 329, 1656
PaeI GCATGC 1 cut(s) 1656
PagI TCATGA 1 cut(s) 1282
PauI GCGCGC 1 cut(s) 1382
PceI AGGCCT 2 cut(s) 1141, 1278
PciSI GCTCTTC 2 cut(s) 495, 1524
PcsI WCGNNNNNNNCGW 2 cut(s) 961, 2560
PctI GAATGC 3 cut(s) 97, 751, 2515
PdmI GAANNNNTTC 2 cut(s) 477, 1777
PfeI GAWTC 7 cut(s) 560, 813, 878, 899, 1300, 1460, 1597
PfoI TCCNGGA 1 cut(s) 2155
PleI GAGTC 1 cut(s) 2264
PpsI GAGTC 1 cut(s) 2264
Ppu21I YACGTR 1 cut(s) 2684
Psp6I CCWGG 5 cut(s) 618, 643, 2155, 2631, 2689
PspGI CCWGG 5 cut(s) 618, 643, 2155, 2631, 2689
PspN4I GGNNCC 5 cut(s) 6, 427, 1698, 1873, 2058
PspPI GGNCC 5 cut(s) 726, 1488, 2629, 2671, 2687
PstI CTGCAG 1 cut(s) 1377
PstNI CAGNNNCTG 2 cut(s) 551, 2869
PsuI RGATCY 2 cut(s) 1573, 2423
PteI GCGCGC 1 cut(s) 1382
PvuII CAGCTG 2 cut(s) 20, 2296
RsaI GTAC 2 cut(s) 1414, 1448
RsaNI GTAC 2 cut(s) 1413, 1447
RseI CAYNNNNRTG 2 cut(s) 894, 1922
SapI GCTCTTC 2 cut(s) 495, 1524
SaqAI TTAA 8 cut(s) 582, 800, 1101, 1736, 1883, 1971, 2240, 2841
Sau3AI GATC 6 cut(s) 226, 1174, 1234, 1573, 1953, 2423
Sau96I GGNCC 5 cut(s) 726, 1488, 2629, 2671, 2687
SchI GAGTC 1 cut(s) 2265
ScrFI CCNGG 5 cut(s) 620, 645, 2157, 2633, 2691
SduI GDGCHC 3 cut(s) 373, 964, 1734
SexAI ACCWGGT 1 cut(s) 2689
SfaNI GCATC 9 cut(s) 228, 438, 946, 1276, 2217, 2476, 2498, 2606, 2791
SfcI CTRYAG 1 cut(s) 1373
SinI GGWCC 5 cut(s) 726, 1488, 2629, 2671, 2687
SmiMI CAYNNNNRTG 2 cut(s) 894, 1922
SmlI CTYRAG 2 cut(s) 1832, 2840
SmoI CTYRAG 2 cut(s) 1832, 2840
SphI GCATGC 1 cut(s) 1656
SseBI AGGCCT 2 cut(s) 1141, 1278
SsiI CCGC 8 cut(s) 39, 510, 700, 734, 2016, 2318, 2834, 2867
SspI AATATT 2 cut(s) 1090, 1353
SspMI CTAG 5 cut(s) 674, 822, 1539, 1649, 2004
StuI AGGCCT 2 cut(s) 1141, 1278
StyD4I CCNGG 5 cut(s) 618, 643, 2155, 2631, 2689
StyI CCWWGG 1 cut(s) 360
TaiI ACGT 1 cut(s) 2686
TaqI TCGA 4 cut(s) 806, 1173, 1227, 1899
TaqII GACCGA 1 cut(s) 219
TatI WGTACW 1 cut(s) 1446
TauI GCSGC 4 cut(s) 703, 2018, 2321, 2869
TfiI GAWTC 7 cut(s) 560, 813, 878, 899, 1300, 1460, 1597
Tru1I TTAA 8 cut(s) 582, 800, 1101, 1736, 1883, 1971, 2240, 2841
Tru9I TTAA 8 cut(s) 582, 800, 1101, 1736, 1883, 1971, 2240, 2841
TscAI CASTG 3 cut(s) 445, 1555, 1929
TseFI GTSAC 4 cut(s) 1064, 2029, 2042, 2882
TseI GCWGC 8 cut(s) 670, 1469, 1685, 2296, 2312, 2315, 2789, 2836
Tsp45I GTSAC 4 cut(s) 1064, 2029, 2042, 2882
TspGWI ACGGA 2 cut(s) 1837, 2159
TspRI CASTG 3 cut(s) 445, 1555, 1929
Vha464I CTTAAG 1 cut(s) 2840
VneI GTGCAC 1 cut(s) 369
VpaK11BI GGWCC 5 cut(s) 726, 1488, 2629, 2671, 2687
XagI CCTNNNNNAGG 2 cut(s) 1059, 1161
XapI RAATTY 4 cut(s) 347, 947, 1901, 1999
XbaI TCTAGA 1 cut(s) 2003
XceI RCATGY 2 cut(s) 329, 1656
XmiI GTMKAC 1 cut(s) 2587
XmnI GAANNNNTTC 2 cut(s) 477, 1777
XspI CTAG 5 cut(s) 674, 822, 1539, 1649, 2004
Zsp2I ATGCAT 3 cut(s) 327, 1658, 2491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.