pycom13g06210

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011009
Physical Location & Seq
Forward (+)
4046722 .. 4047820
1099 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 999 bp
ATGGAAGATCAAGAAAGTAACATTTTATTAGCTTTTTCTTCCCAAGATGGAGTTTTCATGGTACTCTGCATGGTTTTGTCGTGGATATTCATCCACAGATGGAGCCAGAGGAACAAAAAAGGCCCCAAAACATGGCCTCTCGTCGGAGCAGCGATCGAACAGTTCACCAACTACGACAGAATGCACGATTGGCTCGTCAAGTACCTCTCGGAGTCAAACTCCGTCGTCGTCCCAATGCCATTCACAACTTACACTTACATTGCAGATCCTGCGAATGTAGAACATGTCCTCAAGACCAACTTTGCAAACTACCCTAAGGGTGAAGTGTATCGTTCGTACATGGAAGTACTGCTCGGAGATGGGATCTTCAACGTGGATGGAGAGCTTTGGAGGAAACAGAGAAAGACTGCAAGCTTTGAATTTGCGTCCAAGAATTTGAGGGATTTCAGCACTGTGGTTTTCAGAGATTACAGTCTCAAGCTCCATTCGATTCTCAGCCGAGCGTCTTTTCAAGGCCAACAAGTTGATATGCAGGTGGAAACACCTTCGTTACAGCGTCCGCATTGTTGTCGTCTTGATAGAGATGGTTTTACTTCGTTGGACGGTATCAGAAATGAATATATGTTCTTTGTGCAGGAACTGCTGATGAGGATGACTTTGGACTCCATATGCAAGGTGGGATTTGGGGTGGAAATTGGAACTCTGGCTCCCAATCTGCCGGACAATCACTTTGCCAAGGCCTTTGACACTGCAAACATCATCGTGACGCTTCGATTTATCGATCCTCTGTGGAGAATAAAGAGATACTTGAATGTGGGATCAGAAGCTTTACTTGACAAGAGCATTAAAACCATTGATGATTTTACATACTCTGTTATTAGAAGAAGGAAAGCAGAGATCAAAGAAACTCAGCAGGGTTCTGAAAACAACAAGGTAACACAGAACGTTACTTTTTGTTTGCAGGATGAAGAAAACAGATTCATAATCCGATTAACCTAA

Protein Analysis

333

Amino Acids

38.56

Weight (kDa)

7.01

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 41 - 163 2.5e-06 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69500
fragaria_vesca FvH4_4g31080 FvH4_4g31080
malus_domestica MD13G1069300.v1.1 MD16G1069600.v1.1
prunus_persica Prupe.1G277400_v2.0.a1
pyrus_communis pycom13g06210 pycom13g06220
rosa_chinensis RchiOBHm_Chr4g0439461
rosa_laevigata RLG00000006269
rosa_multiflora Rmu_sc0007276.1_g000005
rosa_roxburghii Rroxscaffold_5G00380380
rosa_rugosa Rorug04G0318500
rosa_samantha Rh4AG369800 Rh4BG381600 Rh4BG381700 Rh4CG396300 Rh4DG376200 Rh4DG376300
rosa_wichuraiana Rw4G031530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 523
Acc36I ACCTGC 1 cut(s) 523
AccB7I CCANNNNNTGG 1 cut(s) 132
AciI CCGC 1 cut(s) 560
AclI AACGTT 1 cut(s) 945
AclWI GGATC 4 cut(s) 260, 371, 776, 826
AcsI RAATTY 2 cut(s) 419, 433
AfaI GTAC 4 cut(s) 63, 203, 338, 348
AfiI CCNNNNNNNGG 2 cut(s) 132, 143
AflIII ACRYGT 1 cut(s) 283
AgsI TTSAA 4 cut(s) 370, 419, 512, 811
AjuI GAANNNNNNNTTGG 2 cut(s) 172, 204
AloI GAACNNNNNNTCC 2 cut(s) 691, 723
AluBI AGCT 5 cut(s) 32, 385, 414, 481, 827
AluI AGCT 5 cut(s) 32, 385, 414, 481, 827
Alw26I GTCTC 1 cut(s) 479
AlwI GGATC 4 cut(s) 260, 371, 776, 826
AlwNI CAGNNNCTG 2 cut(s) 269, 640
AoxI GGCC 4 cut(s) 121, 134, 514, 738
ApeKI GCWGC 1 cut(s) 149
ApoI RAATTY 2 cut(s) 419, 433
ArsI GACNNNNNNTTYG 2 cut(s) 713, 745
AspS9I GGNCC 1 cut(s) 122
AsuHPI GGTGA 2 cut(s) 157, 332
AxyI CCTNAGG 1 cut(s) 315
BbvI GCAGC 1 cut(s) 161
BccI CCATC 5 cut(s) 41, 93, 353, 371, 578
BcgI CGANNNNNNTGC 2 cut(s) 551, 585
BcoDI GTCTC 1 cut(s) 479
BfuAI ACCTGC 1 cut(s) 523
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
BmcAI AGTACT 1 cut(s) 348
BmgT120I GGNCC 1 cut(s) 122
BmiI GGNNCC 3 cut(s) 104, 124, 708
BplI GAGNNNNNCTC 2 cut(s) 203, 235
BpuEI CTTGAG 2 cut(s) 275, 461
Bsa29I ATCGAT 1 cut(s) 780
BsaBI GATNNNNATC 1 cut(s) 89
BsaJI CCNNGG 1 cut(s) 735
BsaXI ACNNNNNCTCC 2 cut(s) 691, 721
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 143
Bse21I CCTNAGG 1 cut(s) 315
Bse3DI GCAATG 1 cut(s) 258
Bse8I GATNNNNATC 1 cut(s) 89
BseCI ATCGAT 1 cut(s) 780
BseDI CCNNGG 1 cut(s) 735
BseGI GGATG 4 cut(s) 90, 382, 657, 970
BseJI GATNNNNATC 1 cut(s) 89
BseLI CCNNNNNNNGG 2 cut(s) 132, 143
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 2 cut(s) 508, 923
BseXI GCAGC 1 cut(s) 161
BsgI GTGCAG 1 cut(s) 653
Bsh1285I CGRYCG 1 cut(s) 156
BshFI GGCC 4 cut(s) 123, 136, 516, 740
BshVI ATCGAT 1 cut(s) 780
BsiEI CGRYCG 1 cut(s) 156
BsiSI CCGG 1 cut(s) 719
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 2 cut(s) 132, 143
BsmAI GTCTC 1 cut(s) 479
BsmFI GGGAC 1 cut(s) 215
BsmI GAATGC 1 cut(s) 186
BsnI GGCC 4 cut(s) 123, 136, 516, 740
Bsp143I GATC 7 cut(s) 7, 153, 265, 363, 781, 818, 897
BspACI CCGC 1 cut(s) 560
BspANI GGCC 4 cut(s) 123, 136, 516, 740
BspCNI CTCAG 2 cut(s) 507, 922
BspDI ATCGAT 1 cut(s) 780
BspLI GGNNCC 3 cut(s) 104, 124, 708
BspMI ACCTGC 1 cut(s) 523
BspPI GGATC 4 cut(s) 260, 371, 776, 826
BsrDI GCAATG 1 cut(s) 258
BssECI CCNNGG 1 cut(s) 735
BssMI GATC 7 cut(s) 7, 153, 265, 363, 781, 818, 897
BssT1I CCWWGG 1 cut(s) 735
Bst4CI ACNGT 4 cut(s) 162, 454, 473, 605
BstAPI GCANNNNNTGC 2 cut(s) 269, 640
BstC8I GCNNGC 1 cut(s) 412
BstDEI CTNAG 3 cut(s) 315, 494, 909
BstF5I GGATG 4 cut(s) 90, 382, 657, 970
BstKTI GATC 7 cut(s) 10, 156, 268, 366, 784, 821, 900
BstMAI GTCTC 1 cut(s) 479
BstMBI GATC 7 cut(s) 7, 153, 265, 363, 781, 818, 897
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 3 cut(s) 190, 269, 640
BstNSI RCATGY 1 cut(s) 287
BstV1I GCAGC 1 cut(s) 161
BstX2I RGATCY 2 cut(s) 265, 363
BstYI RGATCY 2 cut(s) 265, 363
Bsu15I ATCGAT 1 cut(s) 780
Bsu36I CCTNAGG 1 cut(s) 315
BsuRI GGCC 4 cut(s) 123, 136, 516, 740
BsuTUI ATCGAT 1 cut(s) 780
BtsCI GGATG 4 cut(s) 90, 382, 657, 970
BtsI GCAGTG 1 cut(s) 747
BtsIMutI CAGTG 2 cut(s) 450, 747
BveI ACCTGC 1 cut(s) 523
Cac8I GCNNGC 1 cut(s) 412
CaiI CAGNNNCTG 2 cut(s) 269, 640
Cfr13I GGNCC 1 cut(s) 122
ClaI ATCGAT 1 cut(s) 780
CseI GACGC 4 cut(s) 414, 492, 545, 775
Csp6I GTAC 4 cut(s) 62, 202, 337, 347
CviAII CATG 5 cut(s) 58, 70, 132, 284, 340
CviQI GTAC 4 cut(s) 62, 202, 337, 347
DdeI CTNAG 3 cut(s) 315, 494, 909
DpnI GATC 7 cut(s) 9, 155, 267, 365, 783, 820, 899
DpnII GATC 7 cut(s) 7, 153, 265, 363, 781, 818, 897
Eco130I CCWWGG 1 cut(s) 735
Eco147I AGGCCT 1 cut(s) 740
Eco81I CCTNAGG 1 cut(s) 315
EcoO109I RGGNCCY 1 cut(s) 122
EcoT14I CCWWGG 1 cut(s) 735
ErhI CCWWGG 1 cut(s) 735
FaeI CATG 5 cut(s) 61, 73, 135, 287, 343
FalI AAGNNNNNCTT 6 cut(s) 284, 316, 791, 823, 816, 848
FaqI GGGAC 1 cut(s) 215
FatI CATG 5 cut(s) 57, 69, 131, 283, 339
FauNDI CATATG 1 cut(s) 668
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 4 cut(s) 77, 389, 664, 977
Fsp4HI GCNGC 1 cut(s) 150
GluI GCNGC 1 cut(s) 150
HaeIII GGCC 4 cut(s) 123, 136, 516, 740
HapII CCGG 1 cut(s) 719
HgaI GACGC 4 cut(s) 414, 492, 545, 775
Hin1II CATG 5 cut(s) 61, 73, 135, 287, 343
HindIII AAGCTT 2 cut(s) 412, 825
HinfI GANTC 4 cut(s) 212, 490, 662, 978
HpaII CCGG 1 cut(s) 719
HphI GGTGA 2 cut(s) 157, 332
Hpy166II GTNNAC 1 cut(s) 165
Hpy188I TCNGA 8 cut(s) 146, 211, 356, 464, 611, 823, 922, 989
Hpy188III TCNNGA 4 cut(s) 11, 292, 575, 763
Hpy8I GTNNAC 1 cut(s) 165
Hpy99I CGWCG 3 cut(s) 146, 227, 230
HpyAV CCTTC 2 cut(s) 555, 879
HpyCH4III ACNGT 4 cut(s) 162, 454, 473, 605
HpyCH4IV ACGT 2 cut(s) 372, 945
HpyF10VI GCNNNNNNNGC 3 cut(s) 190, 269, 640
HpyF3I CTNAG 3 cut(s) 315, 494, 909
HpySE526I ACGT 2 cut(s) 372, 945
Hsp92II CATG 5 cut(s) 61, 73, 135, 287, 343
Kzo9I GATC 7 cut(s) 7, 153, 265, 363, 781, 818, 897
LmnI GCTCC 4 cut(s) 102, 146, 486, 712
LpnPI CCDG 8 cut(s) 119, 282, 518, 620, 689, 732, 899, 947
Lsp1109I GCAGC 1 cut(s) 161
MaeII ACGT 2 cut(s) 372, 945
MaeIII GTNAC 5 cut(s) 17, 549, 763, 934, 946
MalI GATC 7 cut(s) 9, 155, 267, 365, 783, 820, 899
MboI GATC 7 cut(s) 7, 153, 265, 363, 781, 818, 897
MboII GAAGA 5 cut(s) 17, 30, 358, 894, 980
MflI RGATCY 2 cut(s) 265, 363
MluCI AATT 3 cut(s) 419, 433, 693
MlyI GAGTC 2 cut(s) 221, 656
MmeI TCCRAC 2 cut(s) 124, 579
MnlI CCTC 8 cut(s) 102, 147, 215, 299, 384, 432, 642, 795
MseI TTAA 2 cut(s) 846, 992
MslI CAYNNNNRTG 1 cut(s) 761
MspI CCGG 1 cut(s) 719
Mva1269I GAATGC 1 cut(s) 186
MwoI GCNNNNNNNGC 3 cut(s) 190, 269, 640
NdeI CATATG 1 cut(s) 668
NdeII GATC 7 cut(s) 7, 153, 265, 363, 781, 818, 897
NlaIII CATG 5 cut(s) 61, 73, 135, 287, 343
NlaIV GGNNCC 3 cut(s) 104, 124, 708
NmeAIII GCCGAG 1 cut(s) 524
NmuCI GTSAC 1 cut(s) 763
NspI RCATGY 1 cut(s) 287
PaqCI CACCTGC 1 cut(s) 523
PceI AGGCCT 1 cut(s) 740
PciI ACATGT 1 cut(s) 283
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PctI GAATGC 1 cut(s) 186
PfeI GAWTC 2 cut(s) 490, 978
PflMI CCANNNNNTGG 1 cut(s) 132
PkrI GCNGC 1 cut(s) 151
Ple19I CGATCG 1 cut(s) 156
PleI GAGTC 2 cut(s) 220, 656
PpsI GAGTC 2 cut(s) 220, 656
PscI ACATGT 1 cut(s) 283
Psp1406I AACGTT 1 cut(s) 945
PspN4I GGNNCC 3 cut(s) 104, 124, 708
PspPI GGNCC 1 cut(s) 122
PstNI CAGNNNCTG 2 cut(s) 269, 640
PsuI RGATCY 2 cut(s) 265, 363
PvuI CGATCG 1 cut(s) 156
RsaI GTAC 4 cut(s) 63, 203, 338, 348
RsaNI GTAC 4 cut(s) 62, 202, 337, 347
RseI CAYNNNNRTG 1 cut(s) 761
SaqAI TTAA 2 cut(s) 846, 992
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 7 cut(s) 7, 153, 265, 363, 781, 818, 897
Sau96I GGNCC 1 cut(s) 122
ScaI AGTACT 1 cut(s) 348
SchI GAGTC 2 cut(s) 221, 656
SmiMI CAYNNNNRTG 1 cut(s) 761
SmlI CTYRAG 2 cut(s) 290, 476
SmoI CTYRAG 2 cut(s) 290, 476
Sse9I AATT 3 cut(s) 419, 433, 693
SseBI AGGCCT 1 cut(s) 740
SsiI CCGC 1 cut(s) 560
StuI AGGCCT 1 cut(s) 740
StyI CCWWGG 1 cut(s) 735
TaaI ACNGT 4 cut(s) 162, 454, 473, 605
TaiI ACGT 2 cut(s) 375, 948
TaqI TCGA 4 cut(s) 156, 488, 772, 780
TasI AATT 3 cut(s) 419, 433, 693
TatI WGTACW 1 cut(s) 346
TfiI GAWTC 2 cut(s) 490, 978
Tru1I TTAA 2 cut(s) 846, 992
Tru9I TTAA 2 cut(s) 846, 992
TscAI CASTG 2 cut(s) 457, 754
TseFI GTSAC 1 cut(s) 763
TseI GCWGC 1 cut(s) 149
Tsp45I GTSAC 1 cut(s) 763
TspDTI ATGAA 5 cut(s) 46, 79, 630, 970, 981
TspGWI ACGGA 1 cut(s) 211
TspRI CASTG 2 cut(s) 457, 754
Van91I CCANNNNNTGG 1 cut(s) 132
XapI RAATTY 2 cut(s) 419, 433
XceI RCATGY 1 cut(s) 287
XcmI CCANNNNNNNNNTGG 1 cut(s) 673
ZrmI AGTACT 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.