pycom13g11760

Heparan-alpha-glucosaminide N-acetyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003628
Physical Location & Seq
Reverse (-)
8141548 .. 8145804
4257 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1311 bp
ATGAACGACGACGTTGAGATGGCCATCCAGCCGTATCAGTCTCCCGCCGGCGGTGGCGCCGCTTATATGAATAGTAGTAACGAAGAAGCGGCGGTGGCGTCATTGCCTTCGAAGTCGCGGCAACAGCAGCAACGCCTTGGTTCCCTCGACGTCTTTCGCGGACTCACGGTTGTGCTGATGATAATAGTGGACGACATGGGTGGAATCCTGCCCGCAATTAACCATTCGCCCTGGAATGGTTTAACCCTTGCAGATTTAGTCATGCCATTTTTCCTGTTTATGGTTGGTGTTTCCCTTGCACTTACTTACAAGAAACCATCGTGCGGAGTTGCTTCCACGAGAAAAGCAGTTTTACGTACGCTGAAGCTTCTATCATTGGGCCTTTTTCTTCAAGGCGGATTTTTCCATCGTGTCAAGGATCTTACTTTTGGGGTTGATATCACAAAGATGAGATGGATGGGCATTCTACAGAGAATTGCAATTGGATATTTTGTAGCAGCATTGTGCGAGATTTGGCTAAAGCGTGATAGTAATGTCAACTCGGGAAGATCGCTGCTCAGGAAATATCGGTCTCAATTGACGGTGGCTTTGATAATCACCACGCTATATCTATCATTGTTATACGGATTACATGTTCCTGATTGGGAGTACCAGATTCCTGTCGATTCCTCCTCTGCACCAAAAACATTTTCAGTAAAATGTGGAGTACGGGGTGACACAGGACCGGCTTGCAATGCCGTTGGGATGATTGATCGCAAGATATTGGGGATTGAGCATTTATATAAAAGACCAATCTACGCACGAACGGAGGAATGCAGTCTTAACTCCCCCGATTACGGCCCGTTGCCTGCCGATGCTCCTTCTTGGTGCCAAGCCCCTTTTGATCCTGAAGGACTTCTGAGCTCAATGATGGCTATTGTTACCTGCTTGGTTGGTTTGCACTACGGGCACATCATTGTCCATTTTAAGGATCACCGGGAAAGAATCCTACACTGGTCTATCTCATCCTCCTCTCTTGTAGTCTTAGGCCTCGTCCTCGACTTATTTGGCCTGCATATAAACAAGGCACTTTACACATTCAGTTACATGTGCGTCACAGCTGGTGCTGCTGGCATTCTATTTGCTTCCATTTACTTGATGGTTGATGTGTGTGGCTACAGGCGCCCGACTATTGTAATGGAATGGATGGGAATGCATGCACTGATGATTTTCGTGCTCATAGCCTGCAATATCTTGCCAGTTATCCTCTTTGGATTATATTGGGGGAAGCCTGAAAACAACATTCTTAGCTTCGTTGGAATCGGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
DUF1624

Protein Analysis

437

Amino Acids

48.13

Weight (kDa)

8.71

Isoelectric Point (pI)

53.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HGSNAT_cat PF07786 45 - 168 2.3e-06 Heparan-alpha-glucosaminide N-acetyltransferase, catalytic
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 153
Acc36I ACCTGC 1 cut(s) 932
AccB1I GGYRCC 3 cut(s) 56, 867, 1161
AccII CGCG 2 cut(s) 118, 159
AclWI GGATC 3 cut(s) 426, 878, 978
AcoI YGGCCR 1 cut(s) 21
AcuI CTGAAG 2 cut(s) 383, 909
AcyI GRCGYC 4 cut(s) 57, 98, 150, 1162
AfaI GTAC 3 cut(s) 358, 650, 708
AfiI CCNNNNNNNGG 6 cut(s) 50, 236, 280, 323, 836, 967
AflIII ACRYGT 2 cut(s) 631, 1086
AgsI TTSAA 1 cut(s) 392
AjnI CCWGG 1 cut(s) 230
AjuI GAANNNNNNNTTGG 2 cut(s) 673, 705
AleI CACNNNNGTG 1 cut(s) 170
AloI GAACNNNNNNTCC 2 cut(s) 618, 650
AluBI AGCT 4 cut(s) 367, 903, 1100, 1290
AluI AGCT 4 cut(s) 367, 903, 1100, 1290
Alw21I GWGCWC 2 cut(s) 905, 1218
Alw26I GTCTC 2 cut(s) 45, 576
AlwI GGATC 3 cut(s) 426, 878, 978
Ama87I CYCGRG 1 cut(s) 541
AoxI GGCC 5 cut(s) 21, 379, 838, 1027, 1048
ApeKI GCWGC 4 cut(s) 127, 497, 553, 1106
ArsI GACNNNNNNTTYG 2 cut(s) 571, 603
Asp700I GAANNNNTTC 1 cut(s) 894
AspLEI GCGC 2 cut(s) 59, 1164
AspS9I GGNCC 3 cut(s) 379, 722, 839
AsuC2I CCSGG 1 cut(s) 977
AsuHPI GGTGA 3 cut(s) 589, 725, 965
AsuII TTCGAA 1 cut(s) 110
AvaI CYCGRG 1 cut(s) 541
AvaII GGWCC 1 cut(s) 722
BaeGI GKGCMC 1 cut(s) 951
BalI TGGCCA 1 cut(s) 23
BanI GGYRCC 3 cut(s) 56, 867, 1161
BanII GRGCYC 1 cut(s) 905
BauI CACGAG 1 cut(s) 337
Bbv12I GWGCWC 2 cut(s) 905, 1218
BbvI GCAGC 4 cut(s) 139, 509, 540, 1093
BccI CCATC 9 cut(s) 13, 32, 325, 414, 447, 451, 904, 1132, 1180
BceAI ACGGC 3 cut(s) 16, 722, 853
BciT130I CCWGG 1 cut(s) 232
BcnI CCSGG 1 cut(s) 977
BcoDI GTCTC 2 cut(s) 45, 576
BfmI CTRYAG 2 cut(s) 467, 1156
BfoI RGCGCY 2 cut(s) 60, 1165
BfuAI ACCTGC 1 cut(s) 932
BisI GCNGC 7 cut(s) 60, 90, 119, 128, 498, 554, 1107
BlsI GCNGC 7 cut(s) 61, 91, 120, 129, 499, 555, 1108
Bme1390I CCNGG 2 cut(s) 232, 977
Bme18I GGWCC 1 cut(s) 722
BmeT110I CYCGRG 1 cut(s) 541
BmgT120I GGNCC 3 cut(s) 379, 722, 839
BmiI GGNNCC 4 cut(s) 58, 142, 869, 1163
BmrFI CCNGG 2 cut(s) 232, 977
BmsI GCATC 1 cut(s) 844
Bpu10I CCTNAGC 1 cut(s) 557
Bpu14I TTCGAA 1 cut(s) 110
BpuMI CCSGG 1 cut(s) 977
BsaAI YACGTR 1 cut(s) 356
BsaBI GATNNNNATC 1 cut(s) 23
BsaHI GRCGYC 4 cut(s) 57, 98, 150, 1162
BsaI GGTCTC 1 cut(s) 576
BsaJI CCNNGG 2 cut(s) 136, 230
Bsc4I CCNNNNNNNGG 6 cut(s) 50, 236, 280, 323, 836, 967
Bse118I RCCGGY 2 cut(s) 47, 724
Bse1I ACTGG 2 cut(s) 998, 1238
Bse3DI GCAATG 2 cut(s) 101, 739
Bse8I GATNNNNATC 1 cut(s) 23
BseBI CCWGG 1 cut(s) 232
BseDI CCNNGG 2 cut(s) 136, 230
BseGI GGATG 5 cut(s) 24, 462, 750, 1004, 1191
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 6 cut(s) 50, 236, 280, 323, 836, 967
BseMI GCAATG 2 cut(s) 101, 739
BseMII CTCAG 2 cut(s) 571, 890
BseNI ACTGG 2 cut(s) 998, 1238
BseRI GAGGAG 2 cut(s) 661, 1000
BseSI GKGCMC 1 cut(s) 951
BseXI GCAGC 4 cut(s) 139, 509, 540, 1093
BsgI GTGCAG 1 cut(s) 660
Bsh1236I CGCG 2 cut(s) 118, 159
BshFI GGCC 5 cut(s) 23, 381, 840, 1029, 1050
BshNI GGYRCC 3 cut(s) 56, 867, 1161
BsiHKAI GWGCWC 2 cut(s) 905, 1218
BsiHKCI CYCGRG 1 cut(s) 541
BsiSI CCGG 3 cut(s) 48, 725, 976
BsiWI CGTACG 1 cut(s) 356
BslI CCNNNNNNNGG 6 cut(s) 50, 236, 280, 323, 836, 967
BsmAI GTCTC 2 cut(s) 45, 576
BsmI GAATGC 4 cut(s) 462, 818, 1113, 1197
BsnI GGCC 5 cut(s) 23, 381, 840, 1029, 1050
Bso31I GGTCTC 1 cut(s) 576
BsoBI CYCGRG 1 cut(s) 541
Bsp119I TTCGAA 1 cut(s) 110
Bsp1286I GDGCHC 3 cut(s) 905, 951, 1218
Bsp143I GATC 5 cut(s) 418, 548, 751, 883, 970
BspANI GGCC 5 cut(s) 23, 381, 840, 1029, 1050
BspCNI CTCAG 2 cut(s) 570, 891
BspFNI CGCG 2 cut(s) 118, 159
BspLI GGNNCC 4 cut(s) 58, 142, 869, 1163
BspMI ACCTGC 1 cut(s) 932
BspPI GGATC 3 cut(s) 426, 878, 978
BspT104I TTCGAA 1 cut(s) 110
BspT107I GGYRCC 3 cut(s) 56, 867, 1161
BspTNI GGTCTC 1 cut(s) 576
BsrDI GCAATG 2 cut(s) 101, 739
BsrFI RCCGGY 2 cut(s) 47, 724
BsrI ACTGG 2 cut(s) 998, 1238
BssAI RCCGGY 2 cut(s) 47, 724
BssECI CCNNGG 2 cut(s) 136, 230
BssMI GATC 5 cut(s) 418, 548, 751, 883, 970
BssNI GRCGYC 4 cut(s) 57, 98, 150, 1162
BssSI CACGAG 1 cut(s) 337
BssT1I CCWWGG 1 cut(s) 136
Bst2BI CACGAG 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 232
Bst4CI ACNGT 2 cut(s) 169, 583
BstACI GRCGYC 4 cut(s) 57, 98, 150, 1162
BstBAI YACGTR 1 cut(s) 356
BstBI TTCGAA 1 cut(s) 110
BstC8I GCNNGC 8 cut(s) 49, 213, 730, 849, 1052, 1111, 1197, 1225
BstDEI CTNAG 4 cut(s) 557, 899, 1024, 1286
BstF5I GGATG 5 cut(s) 24, 462, 750, 1004, 1191
BstFNI CGCG 2 cut(s) 118, 159
BstH2I RGCGCY 2 cut(s) 60, 1165
BstHHI GCGC 2 cut(s) 59, 1164
BstKTI GATC 5 cut(s) 421, 551, 754, 886, 973
BstMAI GTCTC 2 cut(s) 45, 576
BstMBI GATC 5 cut(s) 418, 548, 751, 883, 970
BstMWI GCNNNNNNNGC 7 cut(s) 95, 124, 127, 734, 946, 1106, 1161
BstNI CCWGG 1 cut(s) 232
BstNSI RCATGY 3 cut(s) 635, 1090, 1199
BstSCI CCNGG 2 cut(s) 230, 975
BstSFI CTRYAG 2 cut(s) 467, 1156
BstSLI GKGCMC 1 cut(s) 951
BstSNI TACGTA 1 cut(s) 356
BstUI CGCG 2 cut(s) 118, 159
BstV1I GCAGC 4 cut(s) 139, 509, 540, 1093
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BsuRI GGCC 5 cut(s) 23, 381, 840, 1029, 1050
BtsCI GGATG 5 cut(s) 24, 462, 750, 1004, 1191
BtsIMutI CAGTG 2 cut(s) 991, 1199
BveI ACCTGC 1 cut(s) 932
Cac8I GCNNGC 8 cut(s) 49, 213, 730, 849, 1052, 1111, 1197, 1225
CfoI GCGC 2 cut(s) 59, 1164
Cfr10I RCCGGY 2 cut(s) 47, 724
Cfr13I GGNCC 3 cut(s) 379, 722, 839
CseI GACGC 2 cut(s) 87, 1081
Csp6I GTAC 3 cut(s) 357, 649, 707
CviAII CATG 5 cut(s) 196, 262, 632, 1087, 1196
CviQI GTAC 3 cut(s) 357, 649, 707
DdeI CTNAG 4 cut(s) 557, 899, 1024, 1286
DinI GGCGCC 2 cut(s) 58, 1163
DpnI GATC 5 cut(s) 420, 550, 753, 885, 972
DpnII GATC 5 cut(s) 418, 548, 751, 883, 970
EaeI YGGCCR 1 cut(s) 21
EciI GGCGGA 1 cut(s) 411
Ecl136II GAGCTC 1 cut(s) 903
Eco105I TACGTA 1 cut(s) 356
Eco130I CCWWGG 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 1029
Eco24I GRGCYC 1 cut(s) 905
Eco31I GGTCTC 1 cut(s) 576
Eco32I GATATC 1 cut(s) 439
Eco47I GGWCC 1 cut(s) 722
Eco53kI GAGCTC 1 cut(s) 903
Eco57I CTGAAG 2 cut(s) 383, 909
Eco88I CYCGRG 1 cut(s) 541
EcoICRI GAGCTC 1 cut(s) 903
EcoRII CCWGG 1 cut(s) 230
EcoRV GATATC 1 cut(s) 439
EcoT14I CCWWGG 1 cut(s) 136
EcoT22I ATGCAT 1 cut(s) 1197
EcoT38I GRGCYC 1 cut(s) 905
EgeI GGCGCC 2 cut(s) 58, 1163
EheI GGCGCC 2 cut(s) 58, 1163
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 5 cut(s) 199, 265, 635, 1090, 1199
FatI CATG 5 cut(s) 195, 261, 631, 1086, 1195
FauI CCCGC 2 cut(s) 52, 220
Fnu4HI GCNGC 7 cut(s) 60, 90, 119, 128, 498, 554, 1107
FokI GGATG 5 cut(s) 11, 469, 757, 991, 1198
FriOI GRGCYC 1 cut(s) 905
Fsp4HI GCNGC 7 cut(s) 60, 90, 119, 128, 498, 554, 1107
GlaI GCGC 2 cut(s) 58, 1163
GluI GCNGC 7 cut(s) 60, 90, 119, 128, 498, 554, 1107
HaeII RGCGCY 2 cut(s) 60, 1165
HaeIII GGCC 5 cut(s) 23, 381, 840, 1029, 1050
HapII CCGG 3 cut(s) 48, 725, 976
HgaI GACGC 2 cut(s) 87, 1081
HhaI GCGC 2 cut(s) 59, 1164
Hin1I GRCGYC 4 cut(s) 57, 98, 150, 1162
Hin1II CATG 5 cut(s) 199, 265, 635, 1090, 1199
Hin6I GCGC 2 cut(s) 57, 1162
HinP1I GCGC 2 cut(s) 57, 1162
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 6 cut(s) 162, 204, 655, 665, 984, 1299
HpaII CCGG 3 cut(s) 48, 725, 976
HphI GGTGA 3 cut(s) 589, 725, 965
Hpy166II GTNNAC 2 cut(s) 190, 538
Hpy188I TCNGA 2 cut(s) 900, 1304
Hpy188III TCNNGA 4 cut(s) 543, 559, 638, 887
Hpy8I GTNNAC 2 cut(s) 190, 538
Hpy99I CGWCG 3 cut(s) 11, 14, 152
HpyAV CCTTC 3 cut(s) 117, 870, 884
HpyCH4III ACNGT 2 cut(s) 169, 583
HpyCH4IV ACGT 3 cut(s) 12, 150, 355
HpyF10VI GCNNNNNNNGC 7 cut(s) 95, 124, 127, 734, 946, 1106, 1161
HpyF3I CTNAG 4 cut(s) 557, 899, 1024, 1286
HpySE526I ACGT 3 cut(s) 12, 150, 355
Hsp92I GRCGYC 4 cut(s) 57, 98, 150, 1162
Hsp92II CATG 5 cut(s) 199, 265, 635, 1090, 1199
HspAI GCGC 2 cut(s) 57, 1162
KasI GGCGCC 2 cut(s) 56, 1161
KroI GCCGGC 1 cut(s) 47
KroNI GCCGGC 1 cut(s) 49
Kzo9I GATC 5 cut(s) 418, 548, 751, 883, 970
LmnI GCTCC 1 cut(s) 862
Lsp1109I GCAGC 4 cut(s) 139, 509, 540, 1093
LweI GCATC 1 cut(s) 844
MaeII ACGT 3 cut(s) 12, 150, 355
MaeIII GTNAC 5 cut(s) 77, 713, 919, 1082, 1093
MalI GATC 5 cut(s) 420, 550, 753, 885, 972
MboI GATC 5 cut(s) 418, 548, 751, 883, 970
MboII GAAGA 3 cut(s) 95, 380, 558
MfeI CAATTG 2 cut(s) 480, 575
MflI RGATCY 1 cut(s) 418
MhlI GDGCHC 3 cut(s) 905, 951, 1218
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 4 cut(s) 216, 474, 480, 575
MluNI TGGCCA 1 cut(s) 23
Mly113I GGCGCC 2 cut(s) 57, 1162
MlyI GAGTC 1 cut(s) 156
MmeI TCCRAC 1 cut(s) 1276
MnlI CCTC 9 cut(s) 155, 679, 682, 802, 1018, 1021, 1040, 1046, 1256
Mox20I TGGCCA 1 cut(s) 23
Mph1103I ATGCAT 1 cut(s) 1197
MreI CGCCGGCG 1 cut(s) 47
MroNI GCCGGC 1 cut(s) 47
MroXI GAANNNNTTC 1 cut(s) 894
MscI TGGCCA 1 cut(s) 23
MseI TTAA 4 cut(s) 219, 242, 822, 966
MslI CAYNNNNRTG 2 cut(s) 170, 446
Msp20I TGGCCA 1 cut(s) 23
MspA1I CMGCKG 1 cut(s) 1100
MspI CCGG 3 cut(s) 48, 725, 976
MspR9I CCNGG 2 cut(s) 232, 977
MunI CAATTG 2 cut(s) 480, 575
Mva1269I GAATGC 4 cut(s) 462, 818, 1113, 1197
MvaI CCWGG 1 cut(s) 232
MvnI CGCG 2 cut(s) 118, 159
MwoI GCNNNNNNNGC 7 cut(s) 95, 124, 127, 734, 946, 1106, 1161
NaeI GCCGGC 1 cut(s) 49
NarI GGCGCC 2 cut(s) 57, 1162
NciI CCSGG 1 cut(s) 977
NdeII GATC 5 cut(s) 418, 548, 751, 883, 970
NgoMIV GCCGGC 1 cut(s) 47
NlaIII CATG 5 cut(s) 199, 265, 635, 1090, 1199
NlaIV GGNNCC 4 cut(s) 58, 142, 869, 1163
NmuCI GTSAC 2 cut(s) 713, 1093
NsiI ATGCAT 1 cut(s) 1197
NspI RCATGY 3 cut(s) 635, 1090, 1199
NspV TTCGAA 1 cut(s) 110
OliI CACNNNNGTG 1 cut(s) 170
PaeI GCATGC 1 cut(s) 1199
PceI AGGCCT 1 cut(s) 1029
PciI ACATGT 2 cut(s) 631, 1086
PctI GAATGC 4 cut(s) 462, 818, 1113, 1197
PdiI GCCGGC 1 cut(s) 49
PdmI GAANNNNTTC 1 cut(s) 894
PfeI GAWTC 5 cut(s) 204, 655, 665, 984, 1299
Pfl23II CGTACG 1 cut(s) 356
PkrI GCNGC 7 cut(s) 61, 91, 120, 129, 499, 555, 1108
PleI GAGTC 1 cut(s) 156
PluTI GGCGCC 2 cut(s) 60, 1165
PpsI GAGTC 1 cut(s) 156
Ppu21I YACGTR 1 cut(s) 356
PscI ACATGT 2 cut(s) 631, 1086
Psp124BI GAGCTC 1 cut(s) 905
Psp6I CCWGG 1 cut(s) 230
PspGI CCWGG 1 cut(s) 230
PspLI CGTACG 1 cut(s) 356
PspN4I GGNNCC 4 cut(s) 58, 142, 869, 1163
PspPI GGNCC 3 cut(s) 379, 722, 839
PsuI RGATCY 1 cut(s) 418
PvuII CAGCTG 1 cut(s) 1100
RsaI GTAC 3 cut(s) 358, 650, 708
RsaNI GTAC 3 cut(s) 357, 649, 707
RseI CAYNNNNRTG 2 cut(s) 170, 446
SacI GAGCTC 1 cut(s) 905
SaqAI TTAA 4 cut(s) 219, 242, 822, 966
SatI GCNGC 7 cut(s) 60, 90, 119, 128, 498, 554, 1107
Sau3AI GATC 5 cut(s) 418, 548, 751, 883, 970
Sau96I GGNCC 3 cut(s) 379, 722, 839
SchI GAGTC 1 cut(s) 156
ScrFI CCNGG 2 cut(s) 232, 977
SduI GDGCHC 3 cut(s) 905, 951, 1218
SetI ASST 8 cut(s) 15, 153, 358, 369, 905, 926, 1102, 1292
SfaNI GCATC 1 cut(s) 844
SfcI CTRYAG 2 cut(s) 467, 1156
SfoI GGCGCC 2 cut(s) 58, 1163
SfuI TTCGAA 1 cut(s) 110
SgrAI CRCCGGYG 1 cut(s) 47
SinI GGWCC 1 cut(s) 722
SmiMI CAYNNNNRTG 2 cut(s) 170, 446
SnaBI TACGTA 1 cut(s) 356
SphI GCATGC 1 cut(s) 1199
Sse9I AATT 4 cut(s) 216, 474, 480, 575
SseBI AGGCCT 1 cut(s) 1029
SspDI GGCGCC 2 cut(s) 56, 1161
SstI GAGCTC 1 cut(s) 905
StuI AGGCCT 1 cut(s) 1029
StyD4I CCNGG 2 cut(s) 230, 975
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 2 cut(s) 169, 583
TaiI ACGT 3 cut(s) 15, 153, 358
TaqI TCGA 4 cut(s) 110, 147, 663, 1038
TaqII GACCGA 1 cut(s) 558
TasI AATT 4 cut(s) 216, 474, 480, 575
TauI GCSGC 3 cut(s) 62, 92, 121
TfiI GAWTC 5 cut(s) 204, 655, 665, 984, 1299
Tru1I TTAA 4 cut(s) 219, 242, 822, 966
Tru9I TTAA 4 cut(s) 219, 242, 822, 966
TscAI CASTG 2 cut(s) 998, 1206
TseFI GTSAC 2 cut(s) 713, 1093
TseI GCWGC 4 cut(s) 127, 497, 553, 1106
Tsp45I GTSAC 2 cut(s) 713, 1093
TspDTI ATGAA 2 cut(s) 17, 83
TspGWI ACGGA 2 cut(s) 639, 821
TspRI CASTG 2 cut(s) 998, 1206
VpaK11BI GGWCC 1 cut(s) 722
XceI RCATGY 3 cut(s) 635, 1090, 1199
XcmI CCANNNNNNNNNTGG 1 cut(s) 1135
XmnI GAANNNNTTC 1 cut(s) 894
ZraI GACGTC 1 cut(s) 151
Zsp2I ATGCAT 1 cut(s) 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.