pycom13g12810

Cleavage inducing molecular chaperone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003374
Physical Location & Seq
Forward (+)
9024605 .. 9026902
2298 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1497 bp
ATGGCTCGGAAGGGTAATCAACAGAAGAATGCTGTAGATCGGCAGACATCAAAGCACAGAAAGAAGGGTTCAGATTCAGGGTGTACACTGGGAGAAACGAAAGGGAATGGGAAGGCAAGTGAGGTGAAAGTTTTTCCTGGAGAGGAACTGCCAAATGGTGACCAGCCAAGCAGTCCTCTTTCAGCCAGTGCAAGTAAAACCAATCATCCTGAAGATGAGAATAAAAGCAAACAAAAGTCTGGAAAGTCTTTGAGGAAAGAGAAGCAAGGTATGGATGCAGTGCAGGGTCCAGAGGAGTCAAGTTCTTTAGGGAGTGATTTAGGAGATCACAATGGGAATAATGAAGCTCCAAGCAGGAGAGAAGAAAATGGGACATTACCTCCGAGCCACTTACGCCGAAAACACACAAAAAGAAAATCTGGTTCCTCCCTGAATAGATGGCATATGAGAACTTTGATAGCTCGATTAGAGTTTTCAGATACCCTATTGTTTAGAAACTTGAAGACATCAACATTGCCGCTCTTGAAGATGGCTAGTGAATGGCTACAGAGGCACAAGCCATTGTTTCTTACCATAAAAAGTAAGGCACTGTATGCCCATGATTATGTCAAAACAAAGTTTGAGCAGGCATATCCATTCGTTTTGAAGTGGCTTATGCAGTTTGGGAGTATATTGTTTCTTTTATCAATGGCCTGGTTGGATTGCACCTTAAGGGGCTTTGATAGCTTAGTTCGGATGGGGACAACTTCCTTCTTTTCAGTTATATGGTGCAGTATTCTCTCAGTAGTTGCTATGGCCGGGATGTTCAAGTTTCTGATTGTGTTGGTCTTTGCTGCTTTGATTGGACTTTTCATTGGGTTCATGCTCTCGATTTTGGTGGTTTCTTTTTGTGGAGCTCTTTTCTTGTGGTTCTATGGAAGCATTTGGACAACAGGATTTATAATCTTCTTAGGAGGTTTGGCATTCACACTGAGCCATGAGCGTATTGCCCTGTCAATTGCCACTGTGTATTCCATTTATTGTGCTTGGACATCTGTTGGATGGCTGGGTTTGCTTTTGGGGCTGAATTTATCTTTTATCTCAAGTGATTATCTGATATATTTCCTAAAGAACAACGTAAGTCAGCAGAGAAGACCCAACGGATCATCTGAGCAAACATCAGGAATGCCAGGTCAACCAGACTTCTTTAACAGTGAGCAGGCGCATACTTCATCCTCCGGAAATGGCCCATGGTTTTCTACTGATCGTAGCCCTGGAGTACCTTCAACCAGTGGCACTGATTCTGAGATAACCTCTGAAGATGAAGTAGTCCGGCTGTTAAACTGTACTGATCACTATTCTGCGTTGGGATTTTCTCGGTACGAGAATGTAGATGTTTCTTTACTGAAGCGGGAGTACAGGAAAAAGGCAATGTTGGTCCATCCTGATAAAAATATGGGAAATGAAAAAGCTGCTGAAGCTTTCAAGAAACTTCAAAATGCATATGAGTTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ Jiv90

Protein Analysis

499

Amino Acids

55.54

Weight (kDa)

9.36

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 444 - 496 3e-12 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 941
AccBSI CCGCTC 1 cut(s) 520
AccIII TCCGGA 1 cut(s) 1217
AciI CCGC 2 cut(s) 518, 1390
AclWI GGATC 1 cut(s) 1150
AcoI YGGCCR 1 cut(s) 795
AcsI RAATTY 1 cut(s) 1066
AcuI CTGAAG 4 cut(s) 231, 1317, 1406, 1476
AfaI GTAC 5 cut(s) 85, 1260, 1327, 1361, 1397
AflII CTTAAG 1 cut(s) 709
AgsI TTSAA 7 cut(s) 502, 526, 646, 808, 1266, 1465, 1475
AjnI CCWGG 4 cut(s) 136, 692, 1168, 1252
AluBI AGCT 6 cut(s) 347, 461, 726, 896, 1451, 1460
AluI AGCT 6 cut(s) 347, 461, 726, 896, 1451, 1460
Alw21I GWGCWC 1 cut(s) 898
AlwI GGATC 1 cut(s) 1150
Aor13HI TCCGGA 1 cut(s) 1217
AoxI GGCC 3 cut(s) 690, 795, 1225
ApeKI GCWGC 2 cut(s) 833, 1451
ApoI RAATTY 1 cut(s) 1066
AspLEI GCGC 1 cut(s) 1204
AspS9I GGNCC 3 cut(s) 287, 1226, 1417
AsuC2I CCSGG 1 cut(s) 799
AsuHPI GGTGA 2 cut(s) 136, 170
AvaII GGWCC 2 cut(s) 287, 1417
BanII GRGCYC 1 cut(s) 898
BarI GAAGNNNNNNTAC 2 cut(s) 1379, 1411
BbsI GAAGAC 2 cut(s) 509, 1138
Bbv12I GWGCWC 1 cut(s) 898
BbvI GCAGC 2 cut(s) 820, 1438
BccI CCATC 5 cut(s) 432, 523, 730, 1035, 1428
BciT130I CCWGG 4 cut(s) 138, 694, 1170, 1254
BclI TGATCA 1 cut(s) 1330
BcnI CCSGG 1 cut(s) 799
BfaI CTAG 1 cut(s) 534
BfmI CTRYAG 2 cut(s) 33, 545
BfrI CTTAAG 1 cut(s) 709
BisI GCNGC 3 cut(s) 518, 834, 1452
BlsI GCNGC 3 cut(s) 519, 835, 1453
Bme1390I CCNGG 5 cut(s) 138, 694, 799, 1170, 1254
Bme18I GGWCC 2 cut(s) 287, 1417
BmgT120I GGNCC 3 cut(s) 287, 1226, 1417
BmiI GGNNCC 2 cut(s) 288, 424
BmrFI CCNGG 5 cut(s) 138, 694, 799, 1170, 1254
BmrI ACTGGG 1 cut(s) 98
BmsI GCATC 1 cut(s) 265
BmuI ACTGGG 1 cut(s) 98
BpiI GAAGAC 2 cut(s) 509, 1138
BplI GAGNNNNNCTC 2 cut(s) 1277, 1309
BpmI CTGGAG 2 cut(s) 159, 1275
BpuEI CTTGAG 1 cut(s) 1066
BpuMI CCSGG 1 cut(s) 799
BsaJI CCNNGG 2 cut(s) 1229, 1252
BsaWI WCCGGW 1 cut(s) 1217
BsaXI ACNNNNNCTCC 2 cut(s) 364, 394
Bse1I ACTGG 3 cut(s) 93, 186, 1269
Bse3DI GCAATG 2 cut(s) 512, 1416
BseAI TCCGGA 1 cut(s) 1217
BseBI CCWGG 4 cut(s) 138, 694, 1170, 1254
BseDI CCNNGG 2 cut(s) 1229, 1252
BseGI GGATG 7 cut(s) 205, 280, 741, 807, 1046, 1211, 1420
BseMI GCAATG 2 cut(s) 512, 1416
BseMII CTCAG 4 cut(s) 795, 962, 1140, 1275
BseNI ACTGG 3 cut(s) 93, 186, 1269
BseRI GAGGAG 1 cut(s) 308
BseXI GCAGC 2 cut(s) 820, 1438
BseYI CCCAGC 1 cut(s) 1045
BsgI GTGCAG 2 cut(s) 302, 790
BshFI GGCC 3 cut(s) 692, 797, 1227
BsiHKAI GWGCWC 1 cut(s) 898
BsiSI CCGG 3 cut(s) 798, 1218, 1312
BslFI GGGAC 2 cut(s) 385, 754
BsmFI GGGAC 2 cut(s) 385, 754
BsmI GAATGC 3 cut(s) 34, 962, 1170
BsnI GGCC 3 cut(s) 692, 797, 1227
Bsp1286I GDGCHC 1 cut(s) 898
Bsp13I TCCGGA 1 cut(s) 1217
Bsp1407I TGTACA 1 cut(s) 83
Bsp143I GATC 5 cut(s) 37, 325, 1142, 1243, 1330
Bsp19I CCATGG 1 cut(s) 1229
BspACI CCGC 2 cut(s) 518, 1390
BspANI GGCC 3 cut(s) 692, 797, 1227
BspCNI CTCAG 4 cut(s) 794, 963, 1141, 1276
BspEI TCCGGA 1 cut(s) 1217
BspLI GGNNCC 2 cut(s) 288, 424
BspPI GGATC 1 cut(s) 1150
BspTI CTTAAG 1 cut(s) 709
BsrBI CCGCTC 1 cut(s) 520
BsrDI GCAATG 2 cut(s) 512, 1416
BsrGI TGTACA 1 cut(s) 83
BsrI ACTGG 3 cut(s) 93, 186, 1269
BssECI CCNNGG 2 cut(s) 1229, 1252
BssMI GATC 5 cut(s) 37, 325, 1142, 1243, 1330
BssT1I CCWWGG 1 cut(s) 1229
Bst2UI CCWGG 4 cut(s) 138, 694, 1170, 1254
Bst4CI ACNGT 4 cut(s) 591, 1006, 1193, 1325
BstAFI CTTAAG 1 cut(s) 709
BstAPI GCANNNNNTGC 1 cut(s) 593
BstAUI TGTACA 1 cut(s) 83
BstC8I GCNNGC 2 cut(s) 627, 1200
BstDEI CTNAG 6 cut(s) 727, 781, 949, 971, 1149, 1284
BstDSI CCRYGG 1 cut(s) 1229
BstEII GGTNACC 1 cut(s) 158
BstF5I GGATG 7 cut(s) 205, 280, 741, 807, 1046, 1211, 1420
BstHHI GCGC 1 cut(s) 1204
BstKTI GATC 5 cut(s) 40, 328, 1145, 1246, 1333
BstMBI GATC 5 cut(s) 37, 325, 1142, 1243, 1330
BstMWI GCNNNNNNNGC 7 cut(s) 393, 550, 593, 723, 1051, 1060, 1457
BstNI CCWGG 4 cut(s) 138, 694, 1170, 1254
BstPI GGTNACC 1 cut(s) 158
BstSCI CCNGG 5 cut(s) 136, 692, 797, 1168, 1252
BstSFI CTRYAG 2 cut(s) 33, 545
BstV1I GCAGC 2 cut(s) 820, 1438
BstV2I GAAGAC 2 cut(s) 509, 1138
BsuRI GGCC 3 cut(s) 692, 797, 1227
BtgI CCRYGG 1 cut(s) 1229
BtsCI GGATG 7 cut(s) 205, 280, 741, 807, 1046, 1211, 1420
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 9 cut(s) 86, 193, 285, 587, 968, 1002, 1198, 1275, 1276
Cac8I GCNNGC 2 cut(s) 627, 1200
CfoI GCGC 1 cut(s) 1204
Cfr13I GGNCC 3 cut(s) 287, 1226, 1417
Csp6I GTAC 5 cut(s) 84, 1259, 1326, 1360, 1396
CviAII CATG 4 cut(s) 599, 862, 977, 1230
CviQI GTAC 5 cut(s) 84, 1259, 1326, 1360, 1396
DdeI CTNAG 6 cut(s) 727, 781, 949, 971, 1149, 1284
DpnI GATC 5 cut(s) 39, 327, 1144, 1245, 1332
DpnII GATC 5 cut(s) 37, 325, 1142, 1243, 1330
EaeI YGGCCR 1 cut(s) 795
Ecl136II GAGCTC 1 cut(s) 896
Eco130I CCWWGG 1 cut(s) 1229
Eco24I GRGCYC 1 cut(s) 898
Eco47I GGWCC 2 cut(s) 287, 1417
Eco53kI GAGCTC 1 cut(s) 896
Eco57I CTGAAG 4 cut(s) 231, 1317, 1406, 1476
Eco91I GGTNACC 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 896
EcoO65I GGTNACC 1 cut(s) 158
EcoRII CCWGG 4 cut(s) 136, 692, 1168, 1252
EcoT14I CCWWGG 1 cut(s) 1229
EcoT22I ATGCAT 1 cut(s) 1483
EcoT38I GRGCYC 1 cut(s) 898
ErhI CCWWGG 1 cut(s) 1229
FaeI CATG 4 cut(s) 602, 865, 980, 1233
FaqI GGGAC 2 cut(s) 385, 754
FatI CATG 4 cut(s) 598, 861, 976, 1229
FauI CCCGC 1 cut(s) 1383
FauNDI CATATG 2 cut(s) 444, 1483
FbaI TGATCA 1 cut(s) 1330
Fnu4HI GCNGC 3 cut(s) 518, 834, 1452
FokI GGATG 7 cut(s) 192, 287, 748, 814, 1053, 1198, 1407
FriOI GRGCYC 1 cut(s) 898
Fsp4HI GCNGC 3 cut(s) 518, 834, 1452
FspBI CTAG 1 cut(s) 534
GlaI GCGC 1 cut(s) 1203
GluI GCNGC 3 cut(s) 518, 834, 1452
GsaI CCCAGC 1 cut(s) 1049
GsuI CTGGAG 2 cut(s) 159, 1275
HaeIII GGCC 3 cut(s) 692, 797, 1227
HapII CCGG 3 cut(s) 798, 1218, 1312
HhaI GCGC 1 cut(s) 1204
Hin1II CATG 4 cut(s) 602, 865, 980, 1233
Hin6I GCGC 1 cut(s) 1202
HinP1I GCGC 1 cut(s) 1202
HincII GTYRAC 1 cut(s) 1175
HindII GTYRAC 1 cut(s) 1175
HindIII AAGCTT 1 cut(s) 1458
HinfI GANTC 3 cut(s) 74, 296, 1280
HpaII CCGG 3 cut(s) 798, 1218, 1312
HphI GGTGA 2 cut(s) 136, 170
Hpy166II GTNNAC 4 cut(s) 84, 86, 1175, 1491
Hpy188III TCNNGA 9 cut(s) 209, 240, 290, 523, 868, 1161, 1218, 1424, 1465
Hpy8I GTNNAC 4 cut(s) 84, 86, 1175, 1491
HpyAV CCTTC 5 cut(s) 4, 58, 106, 760, 1272
HpyCH4III ACNGT 4 cut(s) 591, 1006, 1193, 1325
HpyCH4IV ACGT 1 cut(s) 1116
HpyCH4V TGCA 7 cut(s) 191, 278, 283, 658, 705, 771, 1481
HpyF10VI GCNNNNNNNGC 7 cut(s) 393, 550, 593, 723, 1051, 1060, 1457
HpyF3I CTNAG 6 cut(s) 727, 781, 949, 971, 1149, 1284
HpySE526I ACGT 1 cut(s) 1116
Hsp92II CATG 4 cut(s) 602, 865, 980, 1233
HspAI GCGC 1 cut(s) 1202
Kpn2I TCCGGA 1 cut(s) 1217
Ksp22I TGATCA 1 cut(s) 1330
Kzo9I GATC 5 cut(s) 37, 325, 1142, 1243, 1330
LmnI GCTCC 2 cut(s) 352, 893
Lsp1109I GCAGC 2 cut(s) 820, 1438
LweI GCATC 1 cut(s) 265
MaeI CTAG 1 cut(s) 534
MaeII ACGT 1 cut(s) 1116
MaeIII GTNAC 1 cut(s) 158
MalI GATC 5 cut(s) 39, 327, 1144, 1245, 1332
MbiI CCGCTC 1 cut(s) 520
MboI GATC 5 cut(s) 37, 325, 1142, 1243, 1330
MboII GAAGA 8 cut(s) 37, 224, 374, 514, 538, 937, 1143, 1310
MfeI CAATTG 1 cut(s) 996
MhlI GDGCHC 1 cut(s) 898
MluCI AATT 2 cut(s) 996, 1066
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 2 cut(s) 678, 1018
Mph1103I ATGCAT 1 cut(s) 1483
MroI TCCGGA 1 cut(s) 1217
MseI TTAA 3 cut(s) 710, 1188, 1319
MslI CAYNNNNRTG 1 cut(s) 603
MspCI CTTAAG 1 cut(s) 709
MspI CCGG 3 cut(s) 798, 1218, 1312
MspR9I CCNGG 5 cut(s) 138, 694, 799, 1170, 1254
MunI CAATTG 1 cut(s) 996
Mva1269I GAATGC 3 cut(s) 34, 962, 1170
MvaI CCWGG 4 cut(s) 138, 694, 1170, 1254
MwoI GCNNNNNNNGC 7 cut(s) 393, 550, 593, 723, 1051, 1060, 1457
NciI CCSGG 1 cut(s) 799
NcoI CCATGG 1 cut(s) 1229
NdeI CATATG 2 cut(s) 444, 1483
NdeII GATC 5 cut(s) 37, 325, 1142, 1243, 1330
NlaIII CATG 4 cut(s) 602, 865, 980, 1233
NlaIV GGNNCC 2 cut(s) 288, 424
NmuCI GTSAC 1 cut(s) 158
NsiI ATGCAT 1 cut(s) 1483
PctI GAATGC 3 cut(s) 34, 962, 1170
PfeI GAWTC 2 cut(s) 74, 1280
PfoI TCCNGGA 1 cut(s) 136
PkrI GCNGC 3 cut(s) 519, 835, 1453
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
PsiI TTATAA 1 cut(s) 941
Psp124BI GAGCTC 1 cut(s) 898
Psp6I CCWGG 4 cut(s) 136, 692, 1168, 1252
PspEI GGTNACC 1 cut(s) 158
PspFI CCCAGC 1 cut(s) 1045
PspGI CCWGG 4 cut(s) 136, 692, 1168, 1252
PspN4I GGNNCC 2 cut(s) 288, 424
PspPI GGNCC 3 cut(s) 287, 1226, 1417
RsaI GTAC 5 cut(s) 85, 1260, 1327, 1361, 1397
RsaNI GTAC 5 cut(s) 84, 1259, 1326, 1360, 1396
RseI CAYNNNNRTG 1 cut(s) 603
SacI GAGCTC 1 cut(s) 898
SaqAI TTAA 3 cut(s) 710, 1188, 1319
SatI GCNGC 3 cut(s) 518, 834, 1452
Sau3AI GATC 5 cut(s) 37, 325, 1142, 1243, 1330
Sau96I GGNCC 3 cut(s) 287, 1226, 1417
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 5 cut(s) 138, 694, 799, 1170, 1254
SduI GDGCHC 1 cut(s) 898
SfaNI GCATC 1 cut(s) 265
SfcI CTRYAG 2 cut(s) 33, 545
SinI GGWCC 2 cut(s) 287, 1417
SmiMI CAYNNNNRTG 1 cut(s) 603
SmlI CTYRAG 2 cut(s) 709, 1081
SmoI CTYRAG 2 cut(s) 709, 1081
Sse9I AATT 2 cut(s) 996, 1066
SsiI CCGC 2 cut(s) 518, 1390
SspMI CTAG 1 cut(s) 534
SstI GAGCTC 1 cut(s) 898
StyD4I CCNGG 5 cut(s) 136, 692, 797, 1168, 1252
StyI CCWWGG 1 cut(s) 1229
TaaI ACNGT 4 cut(s) 591, 1006, 1193, 1325
TaiI ACGT 1 cut(s) 1119
TaqI TCGA 2 cut(s) 463, 869
TasI AATT 2 cut(s) 996, 1066
TatI WGTACW 3 cut(s) 83, 1325, 1395
TauI GCSGC 1 cut(s) 520
TfiI GAWTC 2 cut(s) 74, 1280
Tru1I TTAA 3 cut(s) 710, 1188, 1319
Tru9I TTAA 3 cut(s) 710, 1188, 1319
TscAI CASTG 9 cut(s) 93, 193, 285, 594, 975, 1009, 1198, 1276, 1282
TseFI GTSAC 1 cut(s) 158
TseI GCWGC 2 cut(s) 833, 1451
Tsp45I GTSAC 1 cut(s) 158
TspDTI ATGAA 6 cut(s) 357, 841, 850, 1200, 1317, 1458
TspGWI ACGGA 1 cut(s) 1155
TspRI CASTG 9 cut(s) 93, 193, 285, 594, 975, 1009, 1198, 1276, 1282
Vha464I CTTAAG 1 cut(s) 709
VpaK11BI GGWCC 2 cut(s) 287, 1417
XapI RAATTY 1 cut(s) 1066
XspI CTAG 1 cut(s) 534
Zsp2I ATGCAT 1 cut(s) 1483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.