Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0003374

Physical Location & Seq

Genomic Coordinates Reverse (-)

48727969 .. 48733437

Gene Structure 5469 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG267700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1914 bp

ATGGCTCGGAAGGCTAATCAACAGAAGAATGGGGTTGATCGGCAGACGTCAAAGCAAAAGAAGAAGGGTTCGGAGTCGGGGAGTACACCCGCAGAAACGAAAGTGAATGGGAAAGCAAGTGAGGTGAAAGTTTTCCCTGGAGAAGAAATTTCAAATGGTGACCAGCCAAGCAGTCCTCTTTCAGCAGGTGTGAATAAAACCAATCATGCTGGAGATGAGAATAAAAGCAAGCAAAAACCTGGGAAGGTTTTCAGGAAAGAGAATCAAGGGACGGATCCAATGCCGGGTCAAGGGCAGTCGACGTCTTCAGGAAGTGATTCAGGAAATTGCAATGGAAATAATGAAGCTCCAAGCATAAGAGAGCATAATGGGCCGTTACCCCATAGTGAAATACGTCGCAAACATGCAAAAAGAAAATCAGGTTACTCACTGAATCGGCATGTAAAAAATCTGATGGGGAAGATAGAGTCTTCAGATAGCATATTGGTTAGAAATTTGAGGGCATCAACATTGTCCTTCTTGAAAGTGGCTAATGAATGGCTGCAGAGGCACAAACCAATGATTCTGACTGTAACAAGTAAAGTACAGAATGGTCGTGATTATGTCCAAACAAAGGTTGATCAGGCATATCCTATTGTTAAGAAGTGGCTTACGCAGCTTTGGAGTGTGCTGTTTATTATATCAATGCTTTGGTTCAATTTTACCGTAAGGGGTATCGATTCCTTCGTACGCATGGGGACAACATCCTTGTTTTCAGTGATATGGTTCAGCATTCTCTCAGTAGTTTCTATGGTTGGGATGTTTAAATTCCTGATTGTCTTGGTCTCAGCAGCTTTAATTGGAGTTTTCATTGGGTTCATGCTAGCAATTTTATTGGTTGCTCTTTCTGGAGCTCTTTTCTTGTGGCTTTATGGAAGCTTTTGGACAACAGTACTTGTAATCTTCTTTGGAGGTTTGGCATTCACTCTGAGCCATGAGCGGGTTGCACTGTTAATTGCAACTGTGTATTCCATGTATTGTGCTTGGACGTATGTTGGATGGCTGGGTGTGCTCTTGGGGTTAAATTTATCTTTTTTCTCAAGTGAGTGTCTAATATATTTCTTAAAGAACAACGTAAATCATAATACAAGACCTACCGGATCCCCTGAACAAACATCTGGAATGCACGATCAACAAGGCTTCTTCAATGGTGAGCATGTGCATTCCTCATCCTCAGAAAATGGCCCATGGTTTTCTGCTGATCGTAGCCCTGGAGTGCCTTCAACCAGTGGGGCTGATTCTGAGTTAACTTCTGAAGATGAAATTGTTCGGCTGCTAAATTGTACTGACCACTATTCAGTGCTGGGATTCTCTCTCTATGAAGACGTAGATATTTCTTTACTGAAGCGAGAGTATAAGAAAAAGGCAATGTTGGTCCATCCCGATAAGAATATGGGAAATGAAAAGGCTGCTGAAGCTTTCAAGAAACTACAGAATGCATATGAGGTTTTACTTGATTCCTTGAAGCGAAAAGCATATGATGACGAATTAAGGAGCCAGGAACTGCTGAACATTTTCCGTAGGTTTAAAAGTGCTTCACAAAAGAATGGAGGAAACGGTTTCTTCCCCTCTGGATTTGGAAGCCCAGAGGCTGATGGTGAGGACCCATTTGGAGAGTCAAGGCGAATCGCCTGCAATAAGTGCAACAACTTTCATACCTGGTCACTCACTAGAAAATCAAAATCTAGAGCAAGATGGTGCCAGGATTGCAAAGATTTTCATCAAGCGAAAGATGGTGATGGATGGGTTGAACAATCTTCACAACCTTTCTTCTTTGGATTATTGCAGAAGGTACAAGCCTTTATGTTGGAAGCTTGTTTTTGGAATTTGATGTTTCATGTGTCTGCTCATAACTTGATTTCCCTCTTTAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DnaJ Jiv90

Protein Analysis

637

Amino Acids

71.22

Weight (kDa)

9.26

Isoelectric Point (pI)

42.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	443 - 507	3.8e-17	DnaJ domain
Jiv90	PF14901	552 - 614	3.1e-20	Cleavage inducing molecular chaperone

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003374)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49580 AT5G49580 AT5G49580
fragaria_vesca	FvH4_4g20670 FvH4_4g20670 FvH4_4g20670 FvH4_4g20670 FvH4_4g20670 FvH4_4g20670 FvH4_4g20670 FvH4_4g20670 FvH4_4g20670 FvH4_4g20670 FvH4_4g20670
malus_domestica	MD13G1148800.v1.1 MD13G1148900.v1.1 MD16G1149100.v1.1
prunus_persica	Prupe.1G112900_v2.0.a1 Prupe.1G112900_v2.0.a1 Prupe.1G112900_v2.0.a1 Prupe.1G112900_v2.0.a1
pyrus_communis	pycom13g12810 pycom16g12740 pycom16g12770
rosa_chinensis	RchiOBHm_Chr4g0426071
rosa_laevigata	RLG00000007319
rosa_multiflora	Rmu_co8183128.1_g000001 Rmu_ssc0000281.1_g000006
rosa_roxburghii	Rroxscaffold_5G00368080
rosa_rugosa	Rorug04G0208800 Rorug04G0208900
rosa_samantha	Rh4AG265400 Rh4BG271500 Rh4CG284400 Rh4DG267700
rosa_wichuraiana	Rw4G022940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	176
AatII	GACGTC	2 cut(s)	50, 305
Acc36I	ACCTGC	1 cut(s)	176
AccB1I	GGYRCC	1 cut(s)	1737
AccBSI	CCGCTC	1 cut(s)	979
AccI	GTMKAC	1 cut(s)	299
AciI	CCGC	2 cut(s)	90, 979
AclWI	GGATC	4 cut(s)	269, 282, 1134, 1147
AcsI	RAATTY	5 cut(s)	147, 493, 806, 1063, 1864
AcuI	CTGAAG	5 cut(s)	291, 456, 1314, 1403, 1473
AcyI	GRCGYC	2 cut(s)	47, 302
AfaI	GTAC	6 cut(s)	85, 585, 729, 933, 1324, 1833
AfiI	CCNNNNNNNGG	4 cut(s)	284, 290, 613, 979
AgsI	TTSAA	8 cut(s)	153, 523, 697, 1186, 1263, 1462, 1504, 1790
AjnI	CCWGG	6 cut(s)	136, 238, 1249, 1536, 1697, 1740
AluBI	AGCT	7 cut(s)	347, 658, 833, 893, 918, 1457, 1853
AluI	AGCT	7 cut(s)	347, 658, 833, 893, 918, 1457, 1853
Alw21I	GWGCWC	2 cut(s)	895, 1053
Alw26I	GTCTC	1 cut(s)	829
AlwI	GGATC	4 cut(s)	269, 282, 1134, 1147
AlwNI	CAGNNNCTG	2 cut(s)	1543, 1631
AoxI	GGCC	2 cut(s)	371, 1222
ApeKI	GCWGC	5 cut(s)	541, 655, 830, 1312, 1448
ApoI	RAATTY	5 cut(s)	147, 493, 806, 1063, 1864
Asp700I	GAANNNNTTC	5 cut(s)	131, 248, 316, 1305, 1553
AspS9I	GGNCC	4 cut(s)	371, 1223, 1414, 1642
AsuC2I	CCSGG	1 cut(s)	285
AsuHPI	GGTGA	5 cut(s)	136, 170, 1202, 1649, 1787
AsuNHI	GCTAGC	1 cut(s)	862
AvaII	GGWCC	2 cut(s)	1414, 1642
BamHI	GGATCC	2 cut(s)	274, 1139
BanI	GGYRCC	1 cut(s)	1737
BanII	GRGCYC	1 cut(s)	895
BbsI	GAAGAC	3 cut(s)	297, 462, 1368
Bbv12I	GWGCWC	2 cut(s)	895, 1053
BbvI	GCAGC	5 cut(s)	528, 667, 842, 1299, 1435
BccI	CCATC	8 cut(s)	448, 1032, 1425, 1628, 1728, 1766, 1772, 1776
BceAI	ACGGC	1 cut(s)	358
BcgI	CGANNNNNNTGC	2 cut(s)	1653, 1687
BciT130I	CCWGG	6 cut(s)	138, 240, 1251, 1538, 1699, 1742
BclI	TGATCA	1 cut(s)	619
BcnI	CCSGG	1 cut(s)	285
BcoDI	GTCTC	1 cut(s)	829
BfaI	CTAG	3 cut(s)	863, 1710, 1725
BfmI	CTRYAG	2 cut(s)	542, 1469
BfuAI	ACCTGC	1 cut(s)	176
BisI	GCNGC	5 cut(s)	542, 656, 831, 1313, 1449
BlsI	GCNGC	5 cut(s)	543, 657, 832, 1314, 1450
BmcAI	AGTACT	1 cut(s)	933
Bme1390I	CCNGG	7 cut(s)	138, 240, 285, 1251, 1538, 1699, 1742
Bme18I	GGWCC	2 cut(s)	1414, 1642
BmgT120I	GGNCC	4 cut(s)	371, 1223, 1414, 1642
BmiI	GGNNCC	5 cut(s)	276, 1141, 1535, 1644, 1739
BmrFI	CCNGG	7 cut(s)	138, 240, 285, 1251, 1538, 1699, 1742
BmsI	GCATC	1 cut(s)	512
BmtI	GCTAGC	1 cut(s)	866
BpiI	GAAGAC	3 cut(s)	297, 462, 1368
BpmI	CTGGAG	4 cut(s)	159, 231, 909, 1272
BpuEI	CTTGAG	1 cut(s)	1063
BpuMI	CCSGG	1 cut(s)	285
Bsa29I	ATCGAT	1 cut(s)	717
BsaBI	GATNNNNATC	1 cut(s)	1758
BsaHI	GRCGYC	2 cut(s)	47, 302
BsaI	GGTCTC	1 cut(s)	829
BsaJI	CCNNGG	4 cut(s)	136, 239, 1226, 1249
BsaWI	WCCGGW	1 cut(s)	1136
Bsc4I	CCNNNNNNNGG	4 cut(s)	284, 290, 613, 979
Bse1I	ACTGG	1 cut(s)	1266
Bse3DI	GCAATG	2 cut(s)	337, 1413
Bse8I	GATNNNNATC	1 cut(s)	1758
BseBI	CCWGG	6 cut(s)	138, 240, 1251, 1538, 1699, 1742
BseCI	ATCGAT	1 cut(s)	717
BseDI	CCNNGG	4 cut(s)	136, 239, 1226, 1249
BseGI	GGATG	6 cut(s)	743, 804, 1043, 1208, 1417, 1787
BseJI	GATNNNNATC	1 cut(s)	1758
BseLI	CCNNNNNNNGG	4 cut(s)	284, 290, 613, 979
BseMI	GCAATG	2 cut(s)	337, 1413
BseMII	CTCAG	5 cut(s)	792, 840, 959, 1227, 1272
BseNI	ACTGG	1 cut(s)	1266
BseXI	GCAGC	5 cut(s)	528, 667, 842, 1299, 1435
BseYI	CCCAGC	2 cut(s)	1042, 1342
BshFI	GGCC	2 cut(s)	373, 1224
BshNI	GGYRCC	1 cut(s)	1737
BshVI	ATCGAT	1 cut(s)	717
BsiHKAI	GWGCWC	2 cut(s)	895, 1053
BsiSI	CCGG	2 cut(s)	284, 1137
BsiWI	CGTACG	1 cut(s)	727
BslFI	GGGAC	2 cut(s)	283, 751
BslI	CCNNNNNNNGG	4 cut(s)	284, 290, 613, 979
BsmAI	GTCTC	1 cut(s)	829
BsmFI	GGGAC	2 cut(s)	283, 751
BsmI	GAATGC	5 cut(s)	771, 959, 1167, 1201, 1480
BsnI	GGCC	2 cut(s)	373, 1224
Bso31I	GGTCTC	1 cut(s)	829
Bsp1286I	GDGCHC	2 cut(s)	895, 1053
Bsp143I	GATC	6 cut(s)	37, 274, 619, 1139, 1168, 1240
Bsp19I	CCATGG	1 cut(s)	1226
BspACI	CCGC	2 cut(s)	90, 979
BspANI	GGCC	2 cut(s)	373, 1224
BspCNI	CTCAG	5 cut(s)	791, 839, 960, 1226, 1273
BspDI	ATCGAT	1 cut(s)	717
BspLI	GGNNCC	5 cut(s)	276, 1141, 1535, 1644, 1739
BspMAI	CTGCAG	1 cut(s)	546
BspMI	ACCTGC	1 cut(s)	176
BspOI	GCTAGC	1 cut(s)	866
BspPI	GGATC	4 cut(s)	269, 282, 1134, 1147
BspT107I	GGYRCC	1 cut(s)	1737
BspTNI	GGTCTC	1 cut(s)	829
BsrBI	CCGCTC	1 cut(s)	979
BsrDI	GCAATG	2 cut(s)	337, 1413
BsrI	ACTGG	1 cut(s)	1266
BssECI	CCNNGG	4 cut(s)	136, 239, 1226, 1249
BssMI	GATC	6 cut(s)	37, 274, 619, 1139, 1168, 1240
BssNI	GRCGYC	2 cut(s)	47, 302
BssT1I	CCWWGG	1 cut(s)	1226
Bst2UI	CCWGG	6 cut(s)	138, 240, 1251, 1538, 1699, 1742
Bst4CI	ACNGT	6 cut(s)	571, 706, 931, 990, 1003, 1598
BstACI	GRCGYC	2 cut(s)	47, 302
BstAPI	GCANNNNNTGC	1 cut(s)	1680
BstC8I	GCNNGC	3 cut(s)	230, 864, 1672
BstDEI	CTNAG	5 cut(s)	778, 826, 968, 1213, 1281
BstDSI	CCRYGG	1 cut(s)	1226
BstEII	GGTNACC	1 cut(s)	158
BstF5I	GGATG	6 cut(s)	743, 804, 1043, 1208, 1417, 1787
BstKTI	GATC	6 cut(s)	40, 277, 622, 1142, 1171, 1243
BstMAI	GTCTC	1 cut(s)	829
BstMBI	GATC	6 cut(s)	37, 274, 619, 1139, 1168, 1240
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 370, 547, 655, 1048, 1454, 1680, 1746
BstNI	CCWGG	6 cut(s)	138, 240, 1251, 1538, 1699, 1742
BstNSI	RCATGY	3 cut(s)	407, 443, 1199
BstPI	GGTNACC	1 cut(s)	158
BstSCI	CCNGG	7 cut(s)	136, 238, 283, 1249, 1536, 1697, 1740
BstSFI	CTRYAG	2 cut(s)	542, 1469
BstV1I	GCAGC	5 cut(s)	528, 667, 842, 1299, 1435
BstV2I	GAAGAC	3 cut(s)	297, 462, 1368
BstX2I	RGATCY	2 cut(s)	274, 1139
BstYI	RGATCY	2 cut(s)	274, 1139
Bsu15I	ATCGAT	1 cut(s)	717
BsuRI	GGCC	2 cut(s)	373, 1224
BsuTUI	ATCGAT	1 cut(s)	717
BtgI	CCRYGG	1 cut(s)	1226
BtsCI	GGATG	6 cut(s)	743, 804, 1043, 1208, 1417, 1787
BtsIMutI	CAGTG	5 cut(s)	428, 762, 986, 1273, 1344
BveI	ACCTGC	1 cut(s)	176
Cac8I	GCNNGC	3 cut(s)	230, 864, 1672
CaiI	CAGNNNCTG	2 cut(s)	1543, 1631
Cfr13I	GGNCC	4 cut(s)	371, 1223, 1414, 1642
ClaI	ATCGAT	1 cut(s)	717
CsiI	ACCWGGT	1 cut(s)	1697
Csp6I	GTAC	6 cut(s)	84, 584, 728, 932, 1323, 1832
CviQI	GTAC	6 cut(s)	84, 584, 728, 932, 1323, 1832
DdeI	CTNAG	5 cut(s)	778, 826, 968, 1213, 1281
DpnI	GATC	6 cut(s)	39, 276, 621, 1141, 1170, 1242
DpnII	GATC	6 cut(s)	37, 274, 619, 1139, 1168, 1240
DraI	TTTAAA	2 cut(s)	805, 1567
Ecl136II	GAGCTC	1 cut(s)	893
Eco130I	CCWWGG	1 cut(s)	1226
Eco24I	GRGCYC	1 cut(s)	895
Eco31I	GGTCTC	1 cut(s)	829
Eco47I	GGWCC	2 cut(s)	1414, 1642
Eco53kI	GAGCTC	1 cut(s)	893
Eco57I	CTGAAG	5 cut(s)	291, 456, 1314, 1403, 1473
Eco91I	GGTNACC	1 cut(s)	158
EcoICRI	GAGCTC	1 cut(s)	893
EcoO109I	RGGNCCY	1 cut(s)	1642
EcoO65I	GGTNACC	1 cut(s)	158
EcoRII	CCWGG	6 cut(s)	136, 238, 1249, 1536, 1697, 1740
EcoT14I	CCWWGG	1 cut(s)	1226
EcoT22I	ATGCAT	1 cut(s)	1480
EcoT38I	GRGCYC	1 cut(s)	895
ErhI	CCWWGG	1 cut(s)	1226
FaqI	GGGAC	2 cut(s)	283, 751
FauI	CCCGC	2 cut(s)	97, 972
FauNDI	CATATG	2 cut(s)	1480, 1516
FbaI	TGATCA	1 cut(s)	619
FblI	GTMKAC	1 cut(s)	299
Fnu4HI	GCNGC	5 cut(s)	542, 656, 831, 1313, 1449
FokI	GGATG	6 cut(s)	730, 811, 1050, 1195, 1404, 1794
FriOI	GRGCYC	1 cut(s)	895
Fsp4HI	GCNGC	5 cut(s)	542, 656, 831, 1313, 1449
FspBI	CTAG	3 cut(s)	863, 1710, 1725
GluI	GCNGC	5 cut(s)	542, 656, 831, 1313, 1449
GsaI	CCCAGC	2 cut(s)	1046, 1346
GsuI	CTGGAG	4 cut(s)	159, 231, 909, 1272
HaeIII	GGCC	2 cut(s)	373, 1224
HapII	CCGG	2 cut(s)	284, 1137
Hin1I	GRCGYC	2 cut(s)	47, 302
HincII	GTYRAC	2 cut(s)	300, 1287
HindII	GTYRAC	2 cut(s)	300, 1287
HindIII	AAGCTT	3 cut(s)	916, 1455, 1851
HpaI	GTTAAC	1 cut(s)	1287
HpaII	CCGG	2 cut(s)	284, 1137
HphI	GGTGA	5 cut(s)	136, 170, 1202, 1649, 1787
Hpy166II	GTNNAC	3 cut(s)	86, 300, 1287
Hpy188I	TCNGA	9 cut(s)	9, 73, 453, 475, 567, 969, 1216, 1282, 1294
Hpy8I	GTNNAC	3 cut(s)	86, 300, 1287
Hpy99I	CGWCG	2 cut(s)	304, 399
HpyAV	CCTTC	7 cut(s)	4, 58, 238, 526, 733, 1269, 1822
HpyCH4III	ACNGT	6 cut(s)	571, 706, 931, 990, 1003, 1598
HpyCH4IV	ACGT	6 cut(s)	47, 302, 394, 1028, 1113, 1365
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 370, 547, 655, 1048, 1454, 1680, 1746
HpyF3I	CTNAG	5 cut(s)	778, 826, 968, 1213, 1281
HpySE526I	ACGT	6 cut(s)	47, 302, 394, 1028, 1113, 1365
Hsp92I	GRCGYC	2 cut(s)	47, 302
Ksp22I	TGATCA	1 cut(s)	619
KspAI	GTTAAC	1 cut(s)	1287
Kzo9I	GATC	6 cut(s)	37, 274, 619, 1139, 1168, 1240
LmnI	GCTCC	3 cut(s)	352, 890, 1533
Lsp1109I	GCAGC	5 cut(s)	528, 667, 842, 1299, 1435
LweI	GCATC	1 cut(s)	512
MabI	ACCWGGT	1 cut(s)	1697
MaeI	CTAG	3 cut(s)	863, 1710, 1725
MaeII	ACGT	6 cut(s)	47, 302, 394, 1028, 1113, 1365
MaeIII	GTNAC	5 cut(s)	158, 375, 422, 571, 1701
MalI	GATC	6 cut(s)	39, 276, 621, 1141, 1170, 1242
MbiI	CCGCTC	1 cut(s)	979
MboI	GATC	6 cut(s)	37, 274, 619, 1139, 1168, 1240
MflI	RGATCY	2 cut(s)	274, 1139
MhlI	GDGCHC	2 cut(s)	895, 1053
MlyI	GAGTC	3 cut(s)	83, 476, 1664
MmeI	TCCRAC	2 cut(s)	1015, 1827
Mph1103I	ATGCAT	1 cut(s)	1480
MroXI	GAANNNNTTC	5 cut(s)	131, 248, 316, 1305, 1553
MspI	CCGG	2 cut(s)	284, 1137
MspR9I	CCNGG	7 cut(s)	138, 240, 285, 1251, 1538, 1699, 1742
Mva1269I	GAATGC	5 cut(s)	771, 959, 1167, 1201, 1480
MvaI	CCWGG	6 cut(s)	138, 240, 1251, 1538, 1699, 1742
MwoI	GCNNNNNNNGC	8 cut(s)	11, 370, 547, 655, 1048, 1454, 1680, 1746
NciI	CCSGG	1 cut(s)	285
NcoI	CCATGG	1 cut(s)	1226
NdeI	CATATG	2 cut(s)	1480, 1516
NdeII	GATC	6 cut(s)	37, 274, 619, 1139, 1168, 1240
NheI	GCTAGC	1 cut(s)	862
NlaIV	GGNNCC	5 cut(s)	276, 1141, 1535, 1644, 1739
NmuCI	GTSAC	2 cut(s)	158, 1701
NsiI	ATGCAT	1 cut(s)	1480
NspI	RCATGY	3 cut(s)	407, 443, 1199
PaqCI	CACCTGC	1 cut(s)	176
PcsI	WCGNNNNNNNCGW	1 cut(s)	723
PctI	GAATGC	5 cut(s)	771, 959, 1167, 1201, 1480
PdmI	GAANNNNTTC	5 cut(s)	131, 248, 316, 1305, 1553
PfeI	GAWTC	9 cut(s)	262, 317, 433, 562, 719, 1277, 1347, 1496, 1665
Pfl23II	CGTACG	1 cut(s)	727
PkrI	GCNGC	5 cut(s)	543, 657, 832, 1314, 1450
PleI	GAGTC	3 cut(s)	82, 475, 1663
PpsI	GAGTC	3 cut(s)	82, 475, 1663
PpuMI	RGGWCCY	1 cut(s)	1642
Psp124BI	GAGCTC	1 cut(s)	895
Psp5II	RGGWCCY	1 cut(s)	1642
Psp6I	CCWGG	6 cut(s)	136, 238, 1249, 1536, 1697, 1740
PspEI	GGTNACC	1 cut(s)	158
PspFI	CCCAGC	2 cut(s)	1042, 1342
PspGI	CCWGG	6 cut(s)	136, 238, 1249, 1536, 1697, 1740
PspLI	CGTACG	1 cut(s)	727
PspN4I	GGNNCC	5 cut(s)	276, 1141, 1535, 1644, 1739
PspPI	GGNCC	4 cut(s)	371, 1223, 1414, 1642
PspPPI	RGGWCCY	1 cut(s)	1642
PstI	CTGCAG	1 cut(s)	546
PstNI	CAGNNNCTG	2 cut(s)	1543, 1631
PsuI	RGATCY	2 cut(s)	274, 1139
RsaI	GTAC	6 cut(s)	85, 585, 729, 933, 1324, 1833
RsaNI	GTAC	6 cut(s)	84, 584, 728, 932, 1323, 1832
SacI	GAGCTC	1 cut(s)	895
SalI	GTCGAC	1 cut(s)	298
SatI	GCNGC	5 cut(s)	542, 656, 831, 1313, 1449
Sau3AI	GATC	6 cut(s)	37, 274, 619, 1139, 1168, 1240
Sau96I	GGNCC	4 cut(s)	371, 1223, 1414, 1642
ScaI	AGTACT	1 cut(s)	933
SchI	GAGTC	3 cut(s)	83, 476, 1664
ScrFI	CCNGG	7 cut(s)	138, 240, 285, 1251, 1538, 1699, 1742
SduI	GDGCHC	2 cut(s)	895, 1053
SexAI	ACCWGGT	1 cut(s)	1697
SfaNI	GCATC	1 cut(s)	512
SfcI	CTRYAG	2 cut(s)	542, 1469
SinI	GGWCC	2 cut(s)	1414, 1642
SmlI	CTYRAG	1 cut(s)	1078
SmoI	CTYRAG	1 cut(s)	1078
SsiI	CCGC	2 cut(s)	90, 979
SspMI	CTAG	3 cut(s)	863, 1710, 1725
SstI	GAGCTC	1 cut(s)	895
StyD4I	CCNGG	7 cut(s)	136, 238, 283, 1249, 1536, 1697, 1740
StyI	CCWWGG	1 cut(s)	1226
TaaI	ACNGT	6 cut(s)	571, 706, 931, 990, 1003, 1598
TaiI	ACGT	6 cut(s)	50, 305, 397, 1031, 1116, 1368
TaqI	TCGA	2 cut(s)	299, 717
TatI	WGTACW	4 cut(s)	83, 583, 931, 1322
TfiI	GAWTC	9 cut(s)	262, 317, 433, 562, 719, 1277, 1347, 1496, 1665
TscAI	CASTG	5 cut(s)	435, 762, 993, 1273, 1344
TseFI	GTSAC	2 cut(s)	158, 1701
TseI	GCWGC	5 cut(s)	541, 655, 830, 1312, 1448
Tsp45I	GTSAC	2 cut(s)	158, 1701
TspGWI	ACGGA	2 cut(s)	287, 1547
TspRI	CASTG	5 cut(s)	435, 762, 993, 1273, 1344
VpaK11BI	GGWCC	2 cut(s)	1414, 1642
XapI	RAATTY	5 cut(s)	147, 493, 806, 1063, 1864
XbaI	TCTAGA	1 cut(s)	1724
XceI	RCATGY	3 cut(s)	407, 443, 1199
XmiI	GTMKAC	1 cut(s)	299
XmnI	GAANNNNTTC	5 cut(s)	131, 248, 316, 1305, 1553
XspI	CTAG	3 cut(s)	863, 1710, 1725
ZraI	GACGTC	2 cut(s)	48, 303
ZrmI	AGTACT	1 cut(s)	933
Zsp2I	ATGCAT	1 cut(s)	1480

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.