Rorug04G0208900

Cleavage inducing molecular chaperone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003374
Physical Location & Seq
Forward (+)
36450459 .. 36453510
3052 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0208900.1

Sequence Viewer

Length: 1341 bp
ATGGGTAAGGAGAAGTTTCACATCAACATTGTGGTCATTGGCCATGTCGACTCTGGGAAATCGACCACCACTGGTCATCTTATCTACAAGCTTGGAGGTATTGACAAGCGTGTGATTGAGAGGTTTGAGAAGGAAGCTGCTGAGATGAACAAAAGGTCATTCAAGTATGCCTGGGTGCTCGACAAGCTTAAGGCTGAGCGTGAGCGTGGTATTACCATTGATATTGCTTTGTGGAAGTTTGAGACCACAAAGTACTACTGCACTGTCATTGATGCTCCAGGGCATCGTGACTTTATTAAGAACATGATTACTGGAACTTCACAGGCTGACTGTGCTGTTCTCATTATTGATTCCACCACTGGTGGTTTTGAGGCTGGTATTTCCAAGGATGGGCAGACCCGTGAGCATGCTCTTCTTGCTTTCACCCTTGGTGTGAGGCAAATGATTTGCTGCTGTAACAAGATGGATGCTACTACTCCCAAGTACTCAAAGGCAAGGTACGATGAAATTGTGAAGGAAGTCTCATCCTATCTGAAGAAGGTTGGTTACAACCCTGACAAAATTGCCTTCGTTCCCATCTCTGGATTTGAGGGTGACAACATGATTGAGAGGTCCACCAACCTTGACTGGTACAAGGGTCCTACCCTTCTTGAGGCACTCGACCAGATCAACGAGCCAAAGAGGCCCTCAGACAAGCCCCTCCGTCTCCCACTGCAGGATGTCTACAAGATTGGTGGTATTGGAACTGTGCCAGTGGGTCGTGTTGAGACTGGACTTATCAAGCCTGGTATGGTTGTCACTTTTGGTCCAACTGGTCTGACCACTGAAGTTAAGTCTGTTGAGATGCATCACGAGGCTCTTCAGGAGGCTCTCCCTGGTGACAATGTCGGGTTCAATGTTAAGAATGTTGCTGTGAAGGATCTTAAGCGTGGTTTTGTTGCATCCAACTCCAAGGATGATCCTGCAAAGGAGGCTGCCAACTTCACATCCCAGGTCATCATCATGAACCACCCTGGACAGATTGGTAATGGTTATGCCCCAGTTCTTGATTGCCACACTTCTCACATTGCTGTGAAGTTCGGTGAAATCCTCACCAAGATTGACAGGAGGTCTGGTAAGGAACTTGAGAAGGAGCCCAAGTTTTTGAAGAATGGAGATTCTGGTATGGTTAAGATGCTTCCCACCAAGCCTATGGTTGTTGAGACCTTCTCTGAGTACCCACCCTTGGGACGTTTTGCTGTCCGTGACATGAGACAGACTGTTGCTGTTGGTGTTATCAAGAGCGTGGAGAAGAAGGACCCAAGTGGTGCTAAGGTCACCAAATCTGCTGCCAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ Jiv90

Protein Analysis

446

Amino Acids

49.24

Weight (kDa)

9.1

Isoelectric Point (pI)

32.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 7 - 223 1.7e-57 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 248 - 313 7.7e-17 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 322 - 429 1.7e-26 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 327 - 427 9.6e-37 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 48, 723
AclWI GGATC 2 cut(s) 927, 953
AcoI YGGCCR 1 cut(s) 40
AcuI CTGAAG 3 cut(s) 554, 845, 846
AfaI GTAC 5 cut(s) 254, 485, 500, 632, 1217
AfiI CCNNNNNNNGG 6 cut(s) 390, 581, 652, 715, 1225, 1226
AflII CTTAAG 2 cut(s) 188, 923
AgsI TTSAA 3 cut(s) 163, 895, 1147
AhdI GACNNNNNGTC 1 cut(s) 1108
AjnI CCWGG 6 cut(s) 170, 277, 784, 874, 990, 1012
AluBI AGCT 3 cut(s) 91, 137, 187
AluI AGCT 3 cut(s) 91, 137, 187
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 6 cut(s) 236, 526, 710, 761, 1196, 1246
AlwI GGATC 2 cut(s) 927, 953
AoxI GGCC 2 cut(s) 40, 683
ApeKI GCWGC 4 cut(s) 137, 450, 974, 1328
AspS9I GGNCC 5 cut(s) 612, 638, 684, 806, 1297
AsuHPI GGTGA 6 cut(s) 415, 605, 890, 1084, 1094, 1309
AvaII GGWCC 4 cut(s) 612, 638, 806, 1297
BaeI ACNNNNGTAYC 2 cut(s) 622, 655
BalI TGGCCA 1 cut(s) 42
BanII GRGCYC 1 cut(s) 1137
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
BauI CACGAG 1 cut(s) 851
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 4 cut(s) 124, 437, 961, 1315
BccI CCATC 3 cut(s) 383, 457, 584
BciT130I CCWGG 6 cut(s) 172, 279, 786, 876, 992, 1014
BcoDI GTCTC 6 cut(s) 236, 526, 710, 761, 1196, 1246
BfmI CTRYAG 1 cut(s) 713
BfrI CTTAAG 2 cut(s) 188, 923
BglI GCCNNNNNGGC 1 cut(s) 682
BisI GCNGC 4 cut(s) 138, 451, 975, 1329
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 4 cut(s) 139, 452, 976, 1330
BmcAI AGTACT 2 cut(s) 254, 485
Bme1390I CCNGG 6 cut(s) 172, 279, 786, 876, 992, 1014
Bme18I GGWCC 4 cut(s) 612, 638, 806, 1297
BmeRI GACNNNNNGTC 1 cut(s) 1108
BmgT120I GGNCC 5 cut(s) 612, 638, 684, 806, 1297
BmiI GGNNCC 3 cut(s) 639, 1134, 1299
BmrFI CCNGG 6 cut(s) 172, 279, 786, 876, 992, 1014
BmrI ACTGGG 1 cut(s) 1034
BmsI GCATC 7 cut(s) 262, 292, 457, 834, 856, 950, 1164
BmuI ACTGGG 1 cut(s) 1034
BplI GAGNNNNNCTC 2 cut(s) 1193, 1225
BpmI CTGGAG 1 cut(s) 261
Bpu10I CCTNAGC 1 cut(s) 1311
Bpu1102I GCTNAGC 1 cut(s) 195
BpuEI CTTGAG 2 cut(s) 671, 1145
BsaI GGTCTC 2 cut(s) 236, 1196
BsaJI CCNNGG 9 cut(s) 171, 278, 384, 427, 874, 951, 990, 1012, 1224
BsaXI ACNNNNNCTCC 4 cut(s) 1099, 1124, 1129, 1154
Bsc4I CCNNNNNNNGG 6 cut(s) 390, 581, 652, 715, 1225, 1226
Bse1I ACTGG 8 cut(s) 76, 316, 364, 632, 752, 775, 817, 1040
Bse3DI GCAATG 1 cut(s) 1065
BseBI CCWGG 6 cut(s) 172, 279, 786, 876, 992, 1014
BseDI CCNNGG 9 cut(s) 171, 278, 384, 427, 874, 951, 990, 1012, 1224
BseGI GGATG 7 cut(s) 394, 472, 524, 724, 941, 961, 986
BseLI CCNNNNNNNGG 6 cut(s) 390, 581, 652, 715, 1225, 1226
BseMI GCAATG 1 cut(s) 1065
BseMII CTCAG 4 cut(s) 132, 186, 702, 1203
BseNI ACTGG 8 cut(s) 76, 316, 364, 632, 752, 775, 817, 1040
BseXI GCAGC 4 cut(s) 124, 437, 961, 1315
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 2 cut(s) 42, 685
BsiHKAI GWGCWC 1 cut(s) 180
BslFI GGGAC 1 cut(s) 1242
BslI CCNNNNNNNGG 6 cut(s) 390, 581, 652, 715, 1225, 1226
BsmAI GTCTC 6 cut(s) 236, 526, 710, 761, 1196, 1246
BsmBI CGTCTC 1 cut(s) 710
BsmFI GGGAC 1 cut(s) 1242
BsnI GGCC 2 cut(s) 42, 685
Bso31I GGTCTC 2 cut(s) 236, 1196
Bsp1286I GDGCHC 2 cut(s) 180, 1137
Bsp143I GATC 3 cut(s) 666, 919, 958
Bsp1720I GCTNAGC 1 cut(s) 195
BspANI GGCC 2 cut(s) 42, 685
BspCNI CTCAG 4 cut(s) 133, 187, 701, 1204
BspHI TCATGA 1 cut(s) 1002
BspLI GGNNCC 3 cut(s) 639, 1134, 1299
BspMAI CTGCAG 1 cut(s) 717
BspPI GGATC 2 cut(s) 927, 953
BspQI GCTCTTC 2 cut(s) 417, 864
BspTI CTTAAG 2 cut(s) 188, 923
BspTNI GGTCTC 2 cut(s) 236, 1196
BsrDI GCAATG 1 cut(s) 1065
BsrI ACTGG 8 cut(s) 76, 316, 364, 632, 752, 775, 817, 1040
BssECI CCNNGG 9 cut(s) 171, 278, 384, 427, 874, 951, 990, 1012, 1224
BssMI GATC 3 cut(s) 666, 919, 958
BssSI CACGAG 1 cut(s) 851
BssT1I CCWWGG 4 cut(s) 384, 427, 951, 1224
Bst2BI CACGAG 1 cut(s) 851
Bst2UI CCWGG 6 cut(s) 172, 279, 786, 876, 992, 1014
Bst4CI ACNGT 4 cut(s) 265, 332, 748, 1261
Bst6I CTCTTC 2 cut(s) 417, 864
BstAFI CTTAAG 2 cut(s) 188, 923
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 5 cut(s) 141, 195, 688, 1212, 1311
BstEII GGTNACC 1 cut(s) 1315
BstENI CCTNNNNNAGG 1 cut(s) 650
BstF5I GGATG 7 cut(s) 394, 472, 524, 724, 941, 961, 986
BstKTI GATC 3 cut(s) 669, 922, 961
BstMAI GTCTC 6 cut(s) 236, 526, 710, 761, 1196, 1246
BstMBI GATC 3 cut(s) 666, 919, 958
BstMWI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 971
BstNI CCWGG 6 cut(s) 172, 279, 786, 876, 992, 1014
BstNSI RCATGY 1 cut(s) 410
BstPI GGTNACC 1 cut(s) 1315
BstSCI CCNGG 6 cut(s) 170, 277, 784, 874, 990, 1012
BstSFI CTRYAG 1 cut(s) 713
BstV1I GCAGC 4 cut(s) 124, 437, 961, 1315
BstX2I RGATCY 1 cut(s) 919
BstXI CCANNNNNNTGG 1 cut(s) 1192
BstYI RGATCY 1 cut(s) 919
BsuRI GGCC 2 cut(s) 42, 685
BtsCI GGATG 7 cut(s) 394, 472, 524, 724, 941, 961, 986
BtsI GCAGTG 1 cut(s) 710
BtsIMutI CAGTG 6 cut(s) 69, 261, 357, 710, 759, 822
Cac8I GCNNGC 1 cut(s) 408
CciI TCATGA 1 cut(s) 1002
Cfr13I GGNCC 5 cut(s) 612, 638, 684, 806, 1297
Csp6I GTAC 5 cut(s) 253, 484, 499, 631, 1216
CspCI CAANNNNNGTGG 4 cut(s) 604, 639, 715, 750
CviAII CATG 6 cut(s) 44, 304, 407, 601, 1003, 1249
CviQI GTAC 5 cut(s) 253, 484, 499, 631, 1216
DdeI CTNAG 5 cut(s) 141, 195, 688, 1212, 1311
DpnI GATC 3 cut(s) 668, 921, 960
DpnII GATC 3 cut(s) 666, 919, 958
DriI GACNNNNNGTC 1 cut(s) 1108
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 2 cut(s) 417, 864
Eam1105I GACNNNNNGTC 1 cut(s) 1108
EarI CTCTTC 2 cut(s) 417, 864
Eco130I CCWWGG 4 cut(s) 384, 427, 951, 1224
Eco24I GRGCYC 1 cut(s) 1137
Eco31I GGTCTC 2 cut(s) 236, 1196
Eco47I GGWCC 4 cut(s) 612, 638, 806, 1297
Eco57I CTGAAG 3 cut(s) 554, 845, 846
Eco91I GGTNACC 1 cut(s) 1315
EcoNI CCTNNNNNAGG 1 cut(s) 650
EcoO109I RGGNCCY 3 cut(s) 638, 684, 1297
EcoO65I GGTNACC 1 cut(s) 1315
EcoRII CCWGG 6 cut(s) 170, 277, 784, 874, 990, 1012
EcoT14I CCWWGG 4 cut(s) 384, 427, 951, 1224
EcoT22I ATGCAT 1 cut(s) 849
EcoT38I GRGCYC 1 cut(s) 1137
ErhI CCWWGG 4 cut(s) 384, 427, 951, 1224
Esp3I CGTCTC 1 cut(s) 710
FaeI CATG 6 cut(s) 47, 307, 410, 604, 1006, 1252
FaqI GGGAC 1 cut(s) 1242
FatI CATG 6 cut(s) 43, 303, 406, 600, 1002, 1248
FblI GTMKAC 2 cut(s) 48, 723
Fnu4HI GCNGC 4 cut(s) 138, 451, 975, 1329
FokI GGATG 7 cut(s) 401, 479, 511, 731, 928, 968, 973
FriOI GRGCYC 1 cut(s) 1137
Fsp4HI GCNGC 4 cut(s) 138, 451, 975, 1329
GluI GCNGC 4 cut(s) 138, 451, 975, 1329
GsuI CTGGAG 1 cut(s) 261
HaeIII GGCC 2 cut(s) 42, 685
Hin1II CATG 6 cut(s) 47, 307, 410, 604, 1006, 1252
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HindIII AAGCTT 2 cut(s) 89, 185
HinfI GANTC 3 cut(s) 50, 350, 1157
HphI GGTGA 6 cut(s) 415, 605, 890, 1084, 1094, 1309
Hpy166II GTNNAC 3 cut(s) 49, 615, 724
Hpy188I TCNGA 4 cut(s) 534, 691, 819, 1213
Hpy188III TCNNGA 8 cut(s) 287, 582, 650, 851, 863, 1003, 1046, 1279
Hpy8I GTNNAC 3 cut(s) 49, 615, 724
HpyAV CCTTC 9 cut(s) 124, 508, 532, 577, 656, 910, 1123, 1216, 1288
HpyCH4III ACNGT 4 cut(s) 265, 332, 748, 1261
HpyCH4IV ACGT 1 cut(s) 1231
HpyCH4V TGCA 5 cut(s) 261, 715, 847, 941, 965
HpyF10VI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 971
HpyF3I CTNAG 5 cut(s) 141, 195, 688, 1212, 1311
HpySE526I ACGT 1 cut(s) 1231
Hsp92II CATG 6 cut(s) 47, 307, 410, 604, 1006, 1252
Kzo9I GATC 3 cut(s) 666, 919, 958
LguI GCTCTTC 2 cut(s) 417, 864
LmnI GCTCC 2 cut(s) 280, 1132
Lsp1109I GCAGC 4 cut(s) 124, 437, 961, 1315
LweI GCATC 7 cut(s) 262, 292, 457, 834, 856, 950, 1164
MaeII ACGT 1 cut(s) 1231
MaeIII GTNAC 8 cut(s) 287, 455, 545, 593, 796, 878, 1244, 1315
MalI GATC 3 cut(s) 668, 921, 960
MboI GATC 3 cut(s) 666, 919, 958
MboII GAAGA 5 cut(s) 404, 547, 851, 1159, 1303
MflI RGATCY 1 cut(s) 919
MhlI GDGCHC 2 cut(s) 180, 1137
MlsI TGGCCA 1 cut(s) 42
MluCI AATT 2 cut(s) 507, 561
MluNI TGGCCA 1 cut(s) 42
MlyI GAGTC 1 cut(s) 44
MmeI TCCRAC 2 cut(s) 833, 969
Mox20I TGGCCA 1 cut(s) 42
Mph1103I ATGCAT 1 cut(s) 849
MscI TGGCCA 1 cut(s) 42
MseI TTAA 6 cut(s) 189, 297, 831, 900, 924, 1170
MslI CAYNNNNRTG 2 cut(s) 1001, 1070
Msp20I TGGCCA 1 cut(s) 42
MspCI CTTAAG 2 cut(s) 188, 923
MspR9I CCNGG 6 cut(s) 172, 279, 786, 876, 992, 1014
MvaI CCWGG 6 cut(s) 172, 279, 786, 876, 992, 1014
MwoI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 971
NdeII GATC 3 cut(s) 666, 919, 958
NlaIII CATG 6 cut(s) 47, 307, 410, 604, 1006, 1252
NlaIV GGNNCC 3 cut(s) 639, 1134, 1299
NmuCI GTSAC 6 cut(s) 287, 593, 796, 878, 1244, 1315
NsiI ATGCAT 1 cut(s) 849
NspI RCATGY 1 cut(s) 410
PaeI GCATGC 1 cut(s) 410
PagI TCATGA 1 cut(s) 1002
PciSI GCTCTTC 2 cut(s) 417, 864
PfeI GAWTC 2 cut(s) 350, 1157
PflFI GACNNNGTC 1 cut(s) 884
PkrI GCNGC 4 cut(s) 139, 452, 976, 1330
PleI GAGTC 1 cut(s) 44
PpsI GAGTC 1 cut(s) 44
PpuMI RGGWCCY 2 cut(s) 638, 1297
Psp5II RGGWCCY 2 cut(s) 638, 1297
Psp6I CCWGG 6 cut(s) 170, 277, 784, 874, 990, 1012
PspEI GGTNACC 1 cut(s) 1315
PspGI CCWGG 6 cut(s) 170, 277, 784, 874, 990, 1012
PspN4I GGNNCC 3 cut(s) 639, 1134, 1299
PspPI GGNCC 5 cut(s) 612, 638, 684, 806, 1297
PspPPI RGGWCCY 2 cut(s) 638, 1297
PstI CTGCAG 1 cut(s) 717
PsuI RGATCY 1 cut(s) 919
PsyI GACNNNGTC 1 cut(s) 884
RsaI GTAC 5 cut(s) 254, 485, 500, 632, 1217
RsaNI GTAC 5 cut(s) 253, 484, 499, 631, 1216
RseI CAYNNNNRTG 2 cut(s) 1001, 1070
SalI GTCGAC 1 cut(s) 47
SapI GCTCTTC 2 cut(s) 417, 864
SaqAI TTAA 6 cut(s) 189, 297, 831, 900, 924, 1170
SatI GCNGC 4 cut(s) 138, 451, 975, 1329
Sau3AI GATC 3 cut(s) 666, 919, 958
Sau96I GGNCC 5 cut(s) 612, 638, 684, 806, 1297
ScaI AGTACT 2 cut(s) 254, 485
SchI GAGTC 1 cut(s) 44
ScrFI CCNGG 6 cut(s) 172, 279, 786, 876, 992, 1014
SduI GDGCHC 2 cut(s) 180, 1137
SfaNI GCATC 7 cut(s) 262, 292, 457, 834, 856, 950, 1164
SfcI CTRYAG 1 cut(s) 713
SinI GGWCC 4 cut(s) 612, 638, 806, 1297
SmiMI CAYNNNNRTG 2 cut(s) 1001, 1070
SmlI CTYRAG 4 cut(s) 188, 650, 923, 1124
SmoI CTYRAG 4 cut(s) 188, 650, 923, 1124
SphI GCATGC 1 cut(s) 410
Sse9I AATT 2 cut(s) 507, 561
StyD4I CCNGG 6 cut(s) 170, 277, 784, 874, 990, 1012
StyI CCWWGG 4 cut(s) 384, 427, 951, 1224
TaaI ACNGT 4 cut(s) 265, 332, 748, 1261
TaiI ACGT 1 cut(s) 1234
TaqI TCGA 4 cut(s) 48, 62, 180, 660
TasI AATT 2 cut(s) 507, 561
TatI WGTACW 2 cut(s) 252, 483
TfiI GAWTC 2 cut(s) 350, 1157
Tru1I TTAA 6 cut(s) 189, 297, 831, 900, 924, 1170
Tru9I TTAA 6 cut(s) 189, 297, 831, 900, 924, 1170
TscAI CASTG 6 cut(s) 76, 268, 364, 717, 759, 829
TseFI GTSAC 6 cut(s) 287, 593, 796, 878, 1244, 1315
TseI GCWGC 4 cut(s) 137, 450, 974, 1328
Tsp45I GTSAC 6 cut(s) 287, 593, 796, 878, 1244, 1315
TspDTI ATGAA 3 cut(s) 161, 519, 1019
TspGWI ACGGA 2 cut(s) 692, 1232
TspRI CASTG 6 cut(s) 76, 268, 364, 717, 759, 829
Tth111I GACNNNGTC 1 cut(s) 884
Vha464I CTTAAG 2 cut(s) 188, 923
VpaK11BI GGWCC 4 cut(s) 612, 638, 806, 1297
XagI CCTNNNNNAGG 1 cut(s) 650
XceI RCATGY 1 cut(s) 410
XcmI CCANNNNNNNNNTGG 2 cut(s) 50, 1189
XmiI GTMKAC 2 cut(s) 48, 723
ZrmI AGTACT 2 cut(s) 254, 485
Zsp2I ATGCAT 1 cut(s) 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.