pycom13g14290

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010154
Physical Location & Seq
Reverse (-)
10320015 .. 10322039
2025 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1563 bp
ATGGAAGCCAGCTCTTCTTCTGCTTCAATGGTTTTGGGGTCGGTTTCATCCCACGATCAAGAGAGACTGAACTTGAAATCCATATGGGGGGTTGTCGTTTCGGTCAACAACTTCATCCACAAACTCATATCAAATGCCAAAACCAGAAACTCTATCCGGATGAGATGCGCCACCAAGTTTACCACTGCTAGAAAACACAAATTCTTTGAGTTCTCTGAGCAGTCTGTGTTATCGAATTTTTACTGGGGCATTGACAGCATCGAAGCCGCAATTCAAGAAAAGTGTTTGGATGAGAAAGCAGCTCTGCTGAAGAAGGCAGAGCAAATGCTGCAGGTTCCCGCGCTGCTCGACGAGCACGGTGTCACAGCTGGAATTCACAACCGGTATTTGGTCTGCTGCTCTTACTTTTACCTCTCATTGGTCAGGAAGCTCCAGGAGGACGAGTGGCAGGTTGCGCTGCATTTCCTGCAGGCAGTGCTGGTGTCCCCGGGGCTTGTTCAAACAGAATTCGCACCCGAACTCTGCGAAAGGCTCTTCCCATCACGCACCGCCAAGTGTGGGAAGCAAGCAACAAGGGGGAGGGGGAACGCGGGGTCAAATTTTCCCTACGTGGAACATGAGGAAGATGAAGCAATAGGGCGGATGGCGAGGGTGTGGAGAGATTGGCTGATGTATTATCAGGTCATGTTGTACGGAGAGACTCCTCGGTGGGAATGTGACACTGAGCCTCAATATTCTATGCATGAAAAATCTACCAGCTCGTTTTCTTCGAGTACAATTGAGCACTGGGAGGTGCACCCTTTTGATCCTCGAGAGGATATTGGCGGTGGCATGGGAGATGAAATGAAGACTTTTTCCATCTACATCCGGGAATTTGAAGAATATGAGAAAACTGCAAAGGATTTAGATCAAGTGACAAGTTTTAATGTCGAGAAGCTTCAAAGGGGCTCCAGTATCAATAGACTCCACGATATGTTGAATGACTCGCAATCTGATATGCCAACTTCAGTGGGTTCATGCTCTGATTTTTCTGCTTATGACATTGAGTCAGAGGTCATGGATTACGGCGAATGTTCACTGACAACCACAAGAAGTAGTGCAGATTTTCCCAGGTCAGAAATTTGTGACTGGAAGCTGCAGGAGTCAGTGCGTAAAGAAGTCCTGGAAGTAAATAACTCTGCGTTACTTGCCAGTGGATTCGCCAGTTCAATCAAAAGTTTAAACTTAACCCTGTTCGAACACAGAGATAAAAAATCAAAGACCTTTCGGCAGCATTGTATGATCAACCAGATGGATCACCAACAGAGTGGTGCACGAAGAAAGCATAATTTTCGTGGTCACGAAGTAAGTTCACACCCTGCAAAAGACTCCAAAAGTGAACTATTTCATATGACCGAAAAGGCAATATCAAAATTATTGTACTCGGAAGGATTAGGAAAATGGGAGGAAGATCGATGTTGTAGAAGTGACAACAATATACGAAATATTGGGCAAAAAGAACGGAGAAAACTGCGCTGTCTTGAAAGACATGATCTTGGATCAATTGCTAGCAGGCATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

521

Amino Acids

59.66

Weight (kDa)

6.31

Isoelectric Point (pI)

51.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 31 - 181 1.3e-25 E3 ubiquitin-protein ligase LIN-like, ARM repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 322, 439
AccII CGCG 2 cut(s) 341, 590
AccIII TCCGGA 1 cut(s) 156
AciI CCGC 6 cut(s) 267, 339, 549, 590, 640, 825
AclWI GGATC 3 cut(s) 800, 1302, 1546
AcsI RAATTY 7 cut(s) 200, 235, 372, 506, 598, 872, 1119
AcuI CTGAAG 2 cut(s) 329, 990
AfaI GTAC 3 cut(s) 692, 775, 1421
AfiI CCNNNNNNNGG 4 cut(s) 87, 388, 418, 558
AgeI ACCGGT 1 cut(s) 381
AgsI TTSAA 9 cut(s) 27, 76, 275, 500, 878, 941, 979, 1209, 1523
AhdI GACNNNNNGTC 1 cut(s) 1045
AjnI CCWGG 3 cut(s) 432, 1109, 1161
AloI GAACNNNNNNTCC 2 cut(s) 62, 94
AluBI AGCT 7 cut(s) 12, 302, 368, 430, 759, 937, 1135
AluI AGCT 7 cut(s) 12, 302, 368, 430, 759, 937, 1135
Alw21I GWGCWC 4 cut(s) 357, 786, 798, 1315
Alw26I GTCTC 2 cut(s) 58, 692
Alw44I GTGCAC 2 cut(s) 794, 1311
AlwI GGATC 3 cut(s) 800, 1302, 1546
Ama87I CYCGRG 2 cut(s) 487, 810
Aor13HI TCCGGA 1 cut(s) 156
ApaLI GTGCAC 2 cut(s) 794, 1311
ApeKI GCWGC 7 cut(s) 299, 328, 343, 396, 457, 1135, 1270
ApoI RAATTY 7 cut(s) 200, 235, 372, 506, 598, 872, 1119
AsiGI ACCGGT 1 cut(s) 381
Asp700I GAANNNNTTC 1 cut(s) 1383
AspLEI GCGC 4 cut(s) 170, 343, 457, 1515
AsuC2I CCSGG 3 cut(s) 488, 489, 869
AsuHPI GGTGA 1 cut(s) 1289
AsuII TTCGAA 1 cut(s) 1236
AsuNHI GCTAGC 1 cut(s) 1547
AvaI CYCGRG 2 cut(s) 487, 810
BaeGI GKGCMC 2 cut(s) 798, 1315
BanII GRGCYC 1 cut(s) 950
BbsI GAAGAC 1 cut(s) 854
Bbv12I GWGCWC 4 cut(s) 357, 786, 798, 1315
BbvI GCAGC 7 cut(s) 311, 315, 330, 383, 444, 1122, 1282
BccI CCATC 4 cut(s) 547, 637, 866, 1285
BceAI ACGGC 1 cut(s) 1081
BcgI CGANNNNNNTGC 2 cut(s) 1313, 1347
BciT130I CCWGG 3 cut(s) 434, 1111, 1163
BclI TGATCA 1 cut(s) 1281
BcnI CCSGG 3 cut(s) 488, 489, 869
BcoDI GTCTC 2 cut(s) 58, 692
BfaI CTAG 3 cut(s) 189, 1548, 1561
BfmI CTRYAG 3 cut(s) 329, 467, 1136
BfuAI ACCTGC 2 cut(s) 322, 439
BisI GCNGC 8 cut(s) 267, 300, 329, 344, 397, 458, 1136, 1271
BlsI GCNGC 8 cut(s) 268, 301, 330, 345, 398, 459, 1137, 1272
Bme1390I CCNGG 6 cut(s) 434, 488, 489, 869, 1111, 1163
BmeRI GACNNNNNGTC 1 cut(s) 1045
BmeT110I CYCGRG 2 cut(s) 487, 810
BmiI GGNNCC 2 cut(s) 336, 949
BmrFI CCNGG 6 cut(s) 434, 488, 489, 869, 1111, 1163
BmrI ACTGGG 2 cut(s) 253, 796
BmsI GCATC 2 cut(s) 155, 267
BmtI GCTAGC 1 cut(s) 1551
BmuI ACTGGG 2 cut(s) 253, 796
BpiI GAAGAC 1 cut(s) 854
BpmI CTGGAG 2 cut(s) 416, 934
Bpu14I TTCGAA 1 cut(s) 1236
BpuMI CCSGG 3 cut(s) 488, 489, 869
Bsa29I ATCGAT 1 cut(s) 1453
BsaAI YACGTR 1 cut(s) 610
BsaBI GATNNNNATC 1 cut(s) 906
BsaJI CCNNGG 5 cut(s) 486, 487, 488, 704, 1109
BsaWI WCCGGW 2 cut(s) 156, 381
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 388, 418, 558
Bse118I RCCGGY 1 cut(s) 381
Bse1I ACTGG 6 cut(s) 248, 791, 951, 1133, 1191, 1203
Bse8I GATNNNNATC 1 cut(s) 906
BseAI TCCGGA 1 cut(s) 156
BseBI CCWGG 3 cut(s) 434, 1111, 1163
BseCI ATCGAT 1 cut(s) 1453
BseDI CCNNGG 5 cut(s) 486, 487, 488, 704, 1109
BseGI GGATG 6 cut(s) 47, 114, 165, 295, 648, 864
BseJI GATNNNNATC 1 cut(s) 906
BseLI CCNNNNNNNGG 4 cut(s) 87, 388, 418, 558
BseMII CTCAG 2 cut(s) 207, 714
BseNI ACTGG 6 cut(s) 248, 791, 951, 1133, 1191, 1203
BseRI GAGGAG 1 cut(s) 693
BseSI GKGCMC 2 cut(s) 798, 1315
BseXI GCAGC 7 cut(s) 311, 315, 330, 383, 444, 1122, 1282
BsgI GTGCAG 1 cut(s) 1119
Bsh1236I CGCG 2 cut(s) 341, 590
BshTI ACCGGT 1 cut(s) 381
BshVI ATCGAT 1 cut(s) 1453
BsiHKAI GWGCWC 4 cut(s) 357, 786, 798, 1315
BsiHKCI CYCGRG 2 cut(s) 487, 810
BsiSI CCGG 4 cut(s) 157, 382, 488, 868
BslFI GGGAC 1 cut(s) 469
BslI CCNNNNNNNGG 4 cut(s) 87, 388, 418, 558
BsmAI GTCTC 2 cut(s) 58, 692
BsmFI GGGAC 1 cut(s) 469
BsoBI CYCGRG 2 cut(s) 487, 810
Bsp119I TTCGAA 1 cut(s) 1236
Bsp1286I GDGCHC 5 cut(s) 357, 786, 798, 950, 1315
Bsp13I TCCGGA 1 cut(s) 156
Bsp143I GATC 8 cut(s) 55, 805, 907, 1281, 1294, 1450, 1531, 1538
BspACI CCGC 6 cut(s) 267, 339, 549, 590, 640, 825
BspCNI CTCAG 2 cut(s) 208, 715
BspDI ATCGAT 1 cut(s) 1453
BspEI TCCGGA 1 cut(s) 156
BspFNI CGCG 2 cut(s) 341, 590
BspLI GGNNCC 2 cut(s) 336, 949
BspMAI CTGCAG 3 cut(s) 333, 471, 1140
BspMI ACCTGC 2 cut(s) 322, 439
BspOI GCTAGC 1 cut(s) 1551
BspPI GGATC 3 cut(s) 800, 1302, 1546
BspQI GCTCTTC 2 cut(s) 19, 539
BspT104I TTCGAA 1 cut(s) 1236
BsrFI RCCGGY 1 cut(s) 381
BsrI ACTGG 6 cut(s) 248, 791, 951, 1133, 1191, 1203
BssAI RCCGGY 1 cut(s) 381
BssECI CCNNGG 5 cut(s) 486, 487, 488, 704, 1109
BssMI GATC 8 cut(s) 55, 805, 907, 1281, 1294, 1450, 1531, 1538
Bst2UI CCWGG 3 cut(s) 434, 1111, 1163
Bst4CI ACNGT 1 cut(s) 359
Bst6I CTCTTC 2 cut(s) 19, 539
BstAPI GCANNNNNTGC 3 cut(s) 328, 466, 475
BstBAI YACGTR 1 cut(s) 610
BstBI TTCGAA 1 cut(s) 1236
BstC8I GCNNGC 5 cut(s) 10, 471, 567, 1549, 1553
BstDEI CTNAG 2 cut(s) 216, 723
BstF5I GGATG 6 cut(s) 47, 114, 165, 295, 648, 864
BstFNI CGCG 2 cut(s) 341, 590
BstHHI GCGC 4 cut(s) 170, 343, 457, 1515
BstKTI GATC 8 cut(s) 58, 808, 910, 1284, 1297, 1453, 1534, 1541
BstMAI GTCTC 2 cut(s) 58, 692
BstMBI GATC 8 cut(s) 55, 805, 907, 1281, 1294, 1450, 1531, 1538
BstMWI GCNNNNNNNGC 7 cut(s) 255, 328, 352, 454, 466, 475, 1187
BstNI CCWGG 3 cut(s) 434, 1111, 1163
BstSCI CCNGG 6 cut(s) 432, 486, 487, 867, 1109, 1161
BstSFI CTRYAG 3 cut(s) 329, 467, 1136
BstSLI GKGCMC 2 cut(s) 798, 1315
BstUI CGCG 2 cut(s) 341, 590
BstV1I GCAGC 7 cut(s) 311, 315, 330, 383, 444, 1122, 1282
BstV2I GAAGAC 1 cut(s) 854
BstXI CCANNNNNNTGG 1 cut(s) 1307
Bsu15I ATCGAT 1 cut(s) 1453
BsuTUI ATCGAT 1 cut(s) 1453
BtsCI GGATG 6 cut(s) 47, 114, 165, 295, 648, 864
BtsI GCAGTG 2 cut(s) 183, 480
BtsIMutI CAGTG 8 cut(s) 183, 480, 720, 784, 1014, 1076, 1152, 1198
BveI ACCTGC 2 cut(s) 322, 439
Cac8I GCNNGC 5 cut(s) 10, 471, 567, 1549, 1553
CfoI GCGC 4 cut(s) 170, 343, 457, 1515
Cfr10I RCCGGY 1 cut(s) 381
Cfr9I CCCGGG 1 cut(s) 487
ClaI ATCGAT 1 cut(s) 1453
Csp6I GTAC 3 cut(s) 691, 774, 1420
CspAI ACCGGT 1 cut(s) 381
CspCI CAANNNNNGTGG 2 cut(s) 990, 1025
CviAII CATG 7 cut(s) 617, 685, 743, 832, 1017, 1057, 1529
CviQI GTAC 3 cut(s) 691, 774, 1420
DdeI CTNAG 2 cut(s) 216, 723
DpnI GATC 8 cut(s) 57, 807, 909, 1283, 1296, 1452, 1533, 1540
DpnII GATC 8 cut(s) 55, 805, 907, 1281, 1294, 1450, 1531, 1538
DraI TTTAAA 1 cut(s) 1221
DriI GACNNNNNGTC 1 cut(s) 1045
Eam1104I CTCTTC 2 cut(s) 19, 539
Eam1105I GACNNNNNGTC 1 cut(s) 1045
EarI CTCTTC 2 cut(s) 19, 539
EciI GGCGGA 1 cut(s) 655
Eco24I GRGCYC 1 cut(s) 950
Eco57I CTGAAG 2 cut(s) 329, 990
Eco88I CYCGRG 2 cut(s) 487, 810
EcoRI GAATTC 2 cut(s) 372, 506
EcoRII CCWGG 3 cut(s) 432, 1109, 1161
EcoT22I ATGCAT 1 cut(s) 744
EcoT38I GRGCYC 1 cut(s) 950
FaeI CATG 7 cut(s) 620, 688, 746, 835, 1020, 1060, 1532
FaqI GGGAC 1 cut(s) 469
FatI CATG 7 cut(s) 616, 684, 742, 831, 1016, 1056, 1528
FauI CCCGC 2 cut(s) 346, 583
FauNDI CATATG 2 cut(s) 83, 1389
FbaI TGATCA 1 cut(s) 1281
Fnu4HI GCNGC 8 cut(s) 267, 300, 329, 344, 397, 458, 1136, 1271
FokI GGATG 6 cut(s) 34, 101, 172, 302, 655, 851
FriOI GRGCYC 1 cut(s) 950
Fsp4HI GCNGC 8 cut(s) 267, 300, 329, 344, 397, 458, 1136, 1271
FspBI CTAG 3 cut(s) 189, 1548, 1561
GlaI GCGC 4 cut(s) 169, 342, 456, 1514
GluI GCNGC 8 cut(s) 267, 300, 329, 344, 397, 458, 1136, 1271
GsuI CTGGAG 2 cut(s) 416, 934
HapII CCGG 4 cut(s) 157, 382, 488, 868
HhaI GCGC 4 cut(s) 170, 343, 457, 1515
Hin1II CATG 7 cut(s) 620, 688, 746, 835, 1020, 1060, 1532
Hin6I GCGC 4 cut(s) 168, 341, 455, 1513
HinP1I GCGC 4 cut(s) 168, 341, 455, 1513
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HindIII AAGCTT 1 cut(s) 935
HinfI GANTC 7 cut(s) 700, 963, 983, 1046, 1142, 1197, 1367
HpaII CCGG 4 cut(s) 157, 382, 488, 868
HphI GGTGA 1 cut(s) 1289
Hpy166II GTNNAC 7 cut(s) 106, 180, 796, 1076, 1313, 1352, 1379
Hpy188I TCNGA 6 cut(s) 217, 994, 1024, 1051, 1117, 1426
Hpy188III TCNNGA 8 cut(s) 59, 157, 275, 424, 812, 931, 1340, 1520
Hpy8I GTNNAC 7 cut(s) 106, 180, 796, 1076, 1313, 1352, 1379
Hpy99I CGWCG 1 cut(s) 353
HpyAV CCTTC 2 cut(s) 307, 1421
HpyCH4III ACNGT 1 cut(s) 359
HpyCH4IV ACGT 1 cut(s) 609
HpyF10VI GCNNNNNNNGC 7 cut(s) 255, 328, 352, 454, 466, 475, 1187
HpyF3I CTNAG 2 cut(s) 216, 723
HpySE526I ACGT 1 cut(s) 609
Hsp92II CATG 7 cut(s) 620, 688, 746, 835, 1020, 1060, 1532
HspAI GCGC 4 cut(s) 168, 341, 455, 1513
Kpn2I TCCGGA 1 cut(s) 156
Ksp22I TGATCA 1 cut(s) 1281
Kzo9I GATC 8 cut(s) 55, 805, 907, 1281, 1294, 1450, 1531, 1538
LguI GCTCTTC 2 cut(s) 19, 539
LmnI GCTCC 2 cut(s) 435, 953
Lsp1109I GCAGC 7 cut(s) 311, 315, 330, 383, 444, 1122, 1282
LweI GCATC 2 cut(s) 155, 267
MaeI CTAG 3 cut(s) 189, 1548, 1561
MaeII ACGT 1 cut(s) 609
MaeIII GTNAC 7 cut(s) 361, 716, 913, 1124, 1182, 1337, 1466
MalI GATC 8 cut(s) 57, 807, 909, 1283, 1296, 1452, 1533, 1540
MboI GATC 8 cut(s) 55, 805, 907, 1281, 1294, 1450, 1531, 1538
MfeI CAATTG 2 cut(s) 777, 1542
MhlI GDGCHC 5 cut(s) 357, 786, 798, 950, 1315
MlyI GAGTC 6 cut(s) 694, 957, 977, 1055, 1151, 1361
Mph1103I ATGCAT 1 cut(s) 744
MroI TCCGGA 1 cut(s) 156
MroXI GAANNNNTTC 1 cut(s) 1383
MseI TTAA 3 cut(s) 924, 1220, 1226
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 4 cut(s) 157, 382, 488, 868
MspR9I CCNGG 6 cut(s) 434, 488, 489, 869, 1111, 1163
MssI GTTTAAAC 1 cut(s) 1221
MunI CAATTG 2 cut(s) 777, 1542
MvaI CCWGG 3 cut(s) 434, 1111, 1163
MvnI CGCG 2 cut(s) 341, 590
MwoI GCNNNNNNNGC 7 cut(s) 255, 328, 352, 454, 466, 475, 1187
NciI CCSGG 3 cut(s) 488, 489, 869
NdeI CATATG 2 cut(s) 83, 1389
NdeII GATC 8 cut(s) 55, 805, 907, 1281, 1294, 1450, 1531, 1538
NheI GCTAGC 1 cut(s) 1547
NlaIII CATG 7 cut(s) 620, 688, 746, 835, 1020, 1060, 1532
NlaIV GGNNCC 2 cut(s) 336, 949
NmuCI GTSAC 6 cut(s) 361, 716, 913, 1124, 1337, 1466
NsiI ATGCAT 1 cut(s) 744
NspV TTCGAA 1 cut(s) 1236
PaeR7I CTCGAG 1 cut(s) 810
PciSI GCTCTTC 2 cut(s) 19, 539
PcsI WCGNNNNNNNCGW 1 cut(s) 767
PdmI GAANNNNTTC 1 cut(s) 1383
PfeI GAWTC 1 cut(s) 1197
PfoI TCCNGGA 3 cut(s) 432, 867, 1161
PinAI ACCGGT 1 cut(s) 381
PkrI GCNGC 8 cut(s) 268, 301, 330, 345, 398, 459, 1137, 1272
PleI GAGTC 6 cut(s) 694, 957, 977, 1054, 1150, 1361
PmeI GTTTAAAC 1 cut(s) 1221
PpsI GAGTC 6 cut(s) 694, 957, 977, 1054, 1150, 1361
Ppu21I YACGTR 1 cut(s) 610
Psp6I CCWGG 3 cut(s) 432, 1109, 1161
PspGI CCWGG 3 cut(s) 432, 1109, 1161
PspN4I GGNNCC 2 cut(s) 336, 949
PstI CTGCAG 3 cut(s) 333, 471, 1140
PvuII CAGCTG 1 cut(s) 368
RsaI GTAC 3 cut(s) 692, 775, 1421
RsaNI GTAC 3 cut(s) 691, 774, 1420
SapI GCTCTTC 2 cut(s) 19, 539
SaqAI TTAA 3 cut(s) 924, 1220, 1226
SatI GCNGC 8 cut(s) 267, 300, 329, 344, 397, 458, 1136, 1271
Sau3AI GATC 8 cut(s) 55, 805, 907, 1281, 1294, 1450, 1531, 1538
SbfI CCTGCAGG 1 cut(s) 471
SchI GAGTC 6 cut(s) 694, 957, 977, 1055, 1151, 1361
ScrFI CCNGG 6 cut(s) 434, 488, 489, 869, 1111, 1163
SdaI CCTGCAGG 1 cut(s) 471
SduI GDGCHC 5 cut(s) 357, 786, 798, 950, 1315
SfaNI GCATC 2 cut(s) 155, 267
SfcI CTRYAG 3 cut(s) 329, 467, 1136
Sfr274I CTCGAG 1 cut(s) 810
SfuI TTCGAA 1 cut(s) 1236
SlaI CTCGAG 1 cut(s) 810
SmaI CCCGGG 1 cut(s) 489
SmlI CTYRAG 1 cut(s) 810
SmoI CTYRAG 1 cut(s) 810
Sse8387I CCTGCAGG 1 cut(s) 471
SsiI CCGC 6 cut(s) 267, 339, 549, 590, 640, 825
SspI AATATT 2 cut(s) 734, 1486
SspMI CTAG 3 cut(s) 189, 1548, 1561
StyD4I CCNGG 6 cut(s) 432, 486, 487, 867, 1109, 1161
TaaI ACNGT 1 cut(s) 359
TaiI ACGT 1 cut(s) 612
TaqI TCGA 8 cut(s) 233, 261, 348, 770, 811, 930, 1236, 1453
TaqII GACCGA 2 cut(s) 91, 1409
TatI WGTACW 2 cut(s) 773, 1419
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 1 cut(s) 1197
Tru1I TTAA 3 cut(s) 924, 1220, 1226
Tru9I TTAA 3 cut(s) 924, 1220, 1226
TscAI CASTG 8 cut(s) 190, 480, 727, 791, 1014, 1083, 1152, 1198
TseFI GTSAC 6 cut(s) 361, 716, 913, 1124, 1337, 1466
TseI GCWGC 7 cut(s) 299, 328, 343, 396, 457, 1135, 1270
Tsp45I GTSAC 6 cut(s) 361, 716, 913, 1124, 1337, 1466
TspDTI ATGAA 8 cut(s) 36, 103, 642, 759, 855, 860, 1005, 1376
TspGWI ACGGA 2 cut(s) 708, 1516
TspMI CCCGGG 1 cut(s) 487
TspRI CASTG 8 cut(s) 190, 480, 727, 791, 1014, 1083, 1152, 1198
VneI GTGCAC 2 cut(s) 794, 1311
XapI RAATTY 7 cut(s) 200, 235, 372, 506, 598, 872, 1119
XhoI CTCGAG 1 cut(s) 810
XmaI CCCGGG 1 cut(s) 487
XmnI GAANNNNTTC 1 cut(s) 1383
XspI CTAG 3 cut(s) 189, 1548, 1561
Zsp2I ATGCAT 1 cut(s) 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.