pycom13g17290

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011794
Physical Location & Seq
Reverse (-)
13071687 .. 13072161
475 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 354 bp
ATGGGGGTACTGCGCCACCGCCACCGCCACAATCACCATTATATAAGGCTATCAACTGTCATCACGGCCTTCACTTTTCTCTTGTTCACCTCAGCCTCATCGGCCGTAACCAGTCAAACCCAACTCGGTGGTGGGCTTCATCATCAAAGTGAAGGTGGGCCAGCACTGGAGCGAGTCGTGACCCACCAGAAGAGTAGACTGGGTTCATCACCACCGAGTTGCAGATCCAAGTGCGGGAGGTGCTCGCCGTGTAAGGCAGTGCACGTCCCCATCCAACCAGGTGTCTCTATACCTCTCGAGTACTACCCCGAAGCTTGGCGCTGCAAGTGTGGCAACCATCTCTTCATGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

118

Amino Acids

12.93

Weight (kDa)

9.81

Isoelectric Point (pI)

64.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 66 - 117 8e-21 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22820 AT4G14723
fragaria_vesca FvH4_4g01110
malus_domestica MD13G1199400.v1.1 MD16G1199100.v1.1
prunus_persica Prupe.1G020800_v2.0.a1
pyrus_communis pycom13g17290 pycom16g16770
rosa_chinensis RchiOBHm_Chr4g0387661
rosa_laevigata RLG00000010102
rosa_multiflora Rmu_sc0003953.1_g000012
rosa_roxburghii Rroxscaffold_5G00334330
rosa_rugosa Rorug03G0312300 Rorug03G0312400
rosa_samantha Rh4AG017600 Rh4BG012400 Rh4CG018800 Rh4DG013400
rosa_wichuraiana Rw4G001140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 196
AciI CCGC 3 cut(s) 19, 25, 234
AclWI GGATC 1 cut(s) 219
AcoI YGGCCR 1 cut(s) 102
AfaI GTAC 2 cut(s) 9, 302
AfiI CCNNNNNNNGG 2 cut(s) 234, 315
AjiI CACGTC 1 cut(s) 265
AjnI CCWGG 1 cut(s) 277
AluBI AGCT 1 cut(s) 314
AluI AGCT 1 cut(s) 314
Alw21I GWGCWC 2 cut(s) 245, 264
Alw26I GTCTC 1 cut(s) 289
Alw44I GTGCAC 1 cut(s) 260
AlwI GGATC 1 cut(s) 219
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 3 cut(s) 66, 102, 158
ApaLI GTGCAC 1 cut(s) 260
ApeKI GCWGC 1 cut(s) 321
AspLEI GCGC 2 cut(s) 15, 321
AspS9I GGNCC 1 cut(s) 158
AsuHPI GGTGA 3 cut(s) 26, 79, 201
AvaI CYCGRG 1 cut(s) 296
BaeGI GKGCMC 1 cut(s) 264
Bbv12I GWGCWC 2 cut(s) 245, 264
BbvCI CCTCAGC 1 cut(s) 91
BbvI GCAGC 1 cut(s) 308
BccI CCATC 2 cut(s) 278, 345
BceAI ACGGC 3 cut(s) 81, 89, 232
BciT130I CCWGG 1 cut(s) 279
BcoDI GTCTC 1 cut(s) 289
BfaI CTAG 1 cut(s) 352
BfoI RGCGCY 1 cut(s) 322
BglI GCCNNNNNGGC 1 cut(s) 101
BisI GCNGC 1 cut(s) 322
BlsI GCNGC 1 cut(s) 323
BmcAI AGTACT 1 cut(s) 302
Bme1390I CCNGG 1 cut(s) 279
BmeT110I CYCGRG 1 cut(s) 296
BmgBI CACGTC 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 158
BmrFI CCNGG 1 cut(s) 279
BmrI ACTGGG 1 cut(s) 209
BmuI ACTGGG 1 cut(s) 209
BpmI CTGGAG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 91
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 315
Bse1I ACTGG 3 cut(s) 111, 171, 204
BseBI CCWGG 1 cut(s) 279
BseGI GGATG 1 cut(s) 270
BseLI CCNNNNNNNGG 2 cut(s) 234, 315
BseMII CTCAG 1 cut(s) 105
BseNI ACTGG 3 cut(s) 111, 171, 204
BseSI GKGCMC 1 cut(s) 264
BseX3I CGGCCG 1 cut(s) 102
BseXI GCAGC 1 cut(s) 308
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 3 cut(s) 68, 104, 160
BsiEI CGRYCG 1 cut(s) 105
BsiHKAI GWGCWC 2 cut(s) 245, 264
BsiHKCI CYCGRG 1 cut(s) 296
BslFI GGGAC 1 cut(s) 251
BslI CCNNNNNNNGG 2 cut(s) 234, 315
BsmAI GTCTC 1 cut(s) 289
BsmFI GGGAC 1 cut(s) 251
BsnI GGCC 3 cut(s) 68, 104, 160
BsoBI CYCGRG 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 245, 264
Bsp143I GATC 1 cut(s) 224
BspACI CCGC 3 cut(s) 19, 25, 234
BspANI GGCC 3 cut(s) 68, 104, 160
BspCNI CTCAG 1 cut(s) 104
BspPI GGATC 1 cut(s) 219
BsrI ACTGG 3 cut(s) 111, 171, 204
BssMI GATC 1 cut(s) 224
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 1 cut(s) 58
Bst6I CTCTTC 2 cut(s) 185, 347
BstC8I GCNNGC 2 cut(s) 162, 245
BstDEI CTNAG 1 cut(s) 91
BstF5I GGATG 1 cut(s) 270
BstH2I RGCGCY 1 cut(s) 322
BstHHI GCGC 2 cut(s) 15, 321
BstKTI GATC 1 cut(s) 227
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 1 cut(s) 224
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 3 cut(s) 101, 240, 330
BstNI CCWGG 1 cut(s) 279
BstSCI CCNGG 1 cut(s) 277
BstSLI GKGCMC 1 cut(s) 264
BstV1I GCAGC 1 cut(s) 308
BstX2I RGATCY 1 cut(s) 224
BstXI CCANNNNNNTGG 1 cut(s) 128
BstYI RGATCY 1 cut(s) 224
BstZI CGGCCG 1 cut(s) 102
BsuRI GGCC 3 cut(s) 68, 104, 160
BtrI CACGTC 1 cut(s) 265
BtsCI GGATG 1 cut(s) 270
BtsI GCAGTG 1 cut(s) 264
BtsIMutI CAGTG 2 cut(s) 164, 264
Cac8I GCNNGC 2 cut(s) 162, 245
CfoI GCGC 2 cut(s) 15, 321
Cfr13I GGNCC 1 cut(s) 158
CsiI ACCWGGT 1 cut(s) 277
Csp6I GTAC 2 cut(s) 8, 301
CviAII CATG 1 cut(s) 346
CviJI RGCY 7 cut(s) 49, 68, 95, 104, 136, 160, 314
CviKI_1 RGCY 7 cut(s) 49, 68, 95, 104, 136, 160, 314
CviQI GTAC 2 cut(s) 8, 301
DdeI CTNAG 1 cut(s) 91
DpnI GATC 1 cut(s) 226
DpnII GATC 1 cut(s) 224
EaeI YGGCCR 1 cut(s) 102
EagI CGGCCG 1 cut(s) 102
Eam1104I CTCTTC 2 cut(s) 185, 347
EarI CTCTTC 2 cut(s) 185, 347
EclXI CGGCCG 1 cut(s) 102
Eco52I CGGCCG 1 cut(s) 102
Eco88I CYCGRG 1 cut(s) 296
EcoRII CCWGG 1 cut(s) 277
FaeI CATG 1 cut(s) 349
FaiI YATR 4 cut(s) 42, 44, 290, 347
FaqI GGGAC 1 cut(s) 251
FatI CATG 1 cut(s) 345
FauI CCCGC 1 cut(s) 227
FblI GTMKAC 1 cut(s) 196
Fnu4HI GCNGC 1 cut(s) 322
FokI GGATG 1 cut(s) 257
Fsp4HI GCNGC 1 cut(s) 322
FspBI CTAG 1 cut(s) 352
GlaI GCGC 2 cut(s) 14, 320
GluI GCNGC 1 cut(s) 322
GsuI CTGGAG 1 cut(s) 188
HaeII RGCGCY 1 cut(s) 322
HaeIII GGCC 3 cut(s) 68, 104, 160
HhaI GCGC 2 cut(s) 15, 321
Hin1II CATG 1 cut(s) 349
Hin6I GCGC 2 cut(s) 13, 319
HinP1I GCGC 2 cut(s) 13, 319
HindIII AAGCTT 1 cut(s) 312
HinfI GANTC 1 cut(s) 174
HphI GGTGA 3 cut(s) 26, 79, 201
Hpy166II GTNNAC 3 cut(s) 87, 197, 262
Hpy188III TCNNGA 2 cut(s) 178, 296
Hpy8I GTNNAC 3 cut(s) 87, 197, 262
HpyAV CCTTC 2 cut(s) 79, 146
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 3 cut(s) 222, 262, 324
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 240, 330
HpyF3I CTNAG 1 cut(s) 91
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 1 cut(s) 349
HspAI GCGC 2 cut(s) 13, 319
Kzo9I GATC 1 cut(s) 224
LmnI GCTCC 1 cut(s) 169
LpnPI CCDG 7 cut(s) 124, 152, 174, 185, 200, 264, 291
Lsp1109I GCAGC 1 cut(s) 308
MabI ACCWGGT 1 cut(s) 277
MaeI CTAG 1 cut(s) 352
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 2 cut(s) 106, 178
MalI GATC 1 cut(s) 226
MboI GATC 1 cut(s) 224
MboII GAAGA 2 cut(s) 202, 334
MflI RGATCY 1 cut(s) 224
MhlI GDGCHC 2 cut(s) 245, 264
MlyI GAGTC 1 cut(s) 183
MmeI TCCRAC 1 cut(s) 298
MnlI CCTC 4 cut(s) 100, 106, 231, 303
MslI CAYNNNNRTG 1 cut(s) 147
MspR9I CCNGG 1 cut(s) 279
MvaI CCWGG 1 cut(s) 279
MwoI GCNNNNNNNGC 3 cut(s) 101, 240, 330
NdeII GATC 1 cut(s) 224
NlaIII CATG 1 cut(s) 349
NmuCI GTSAC 1 cut(s) 178
PaeR7I CTCGAG 1 cut(s) 296
PkrI GCNGC 1 cut(s) 323
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 277
PspGI CCWGG 1 cut(s) 277
PspPI GGNCC 1 cut(s) 158
PsrI GAACNNNNNNTAC 2 cut(s) 187, 219
PsuI RGATCY 1 cut(s) 224
RsaI GTAC 2 cut(s) 9, 302
RsaNI GTAC 2 cut(s) 8, 301
RseI CAYNNNNRTG 1 cut(s) 147
SatI GCNGC 1 cut(s) 322
Sau3AI GATC 1 cut(s) 224
Sau96I GGNCC 1 cut(s) 158
ScaI AGTACT 1 cut(s) 302
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 1 cut(s) 279
SduI GDGCHC 2 cut(s) 245, 264
SetI ASST 7 cut(s) 92, 157, 242, 267, 283, 295, 316
SexAI ACCWGGT 1 cut(s) 277
Sfr274I CTCGAG 1 cut(s) 296
SlaI CTCGAG 1 cut(s) 296
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SsiI CCGC 3 cut(s) 19, 25, 234
SspMI CTAG 1 cut(s) 352
StyD4I CCNGG 1 cut(s) 277
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 1 cut(s) 267
TaqI TCGA 1 cut(s) 297
TatI WGTACW 1 cut(s) 300
TscAI CASTG 2 cut(s) 171, 264
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 1 cut(s) 321
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 3 cut(s) 128, 195, 334
TspRI CASTG 2 cut(s) 171, 264
VneI GTGCAC 1 cut(s) 260
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XhoI CTCGAG 1 cut(s) 296
XmiI GTMKAC 1 cut(s) 196
XspI CTAG 1 cut(s) 352
ZrmI AGTACT 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.